Faculty Profiles - akihide Tanimoto

Papers - akihide Tanimoto

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山田壮亮, 谷本昭英, 島尻正平, 笹栗靖之 . 腎のmucinous tubular and spindle cell carcinomaの1症例 . 診断病理25 ( 2 ) 121 - 124 2008.4

Publisher：(一社)日本病理学会

65歳女性の左腎上極に発生したmucinous tubular and spindle cell carcinoma(MTSC)の1例を経験したので報告する。生来健康であったが、健診時に偶然左腎腫瘍を指摘され、悪性を完全に否定できないため、左腎摘出術を施行された。腫瘍は径3.5×3×3cmの大きさで腎皮質に限局する境界明瞭な白色調の病変であり、内部に出血・壊死を認めなかった。組織学的に、腫瘍は主に管状に増殖し、間質には泡状変化の目立つムチンの沈着を伴っていた。一部では、紡錘形細胞の錯綜性増殖も見られたが、いずれにおいても、腫瘍細胞は異型の弱い小型細胞であった。鑑別としてpapillary renal cell carcinoma(papillary RCC)が挙げられたが、細胞異型が弱いこと、ムチンの沈着が目立つことなどより、MTSCと診断した。(著者抄録)

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2008&ichushi_jid=J03623&link_issn=&doc_id=20080515230014&doc_link_id=%2Fcd9jjodp%2F2008%2F002502%2F014%2F0121-0124%26dl%3D0&url=http%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcd9jjodp%2F2008%2F002502%2F014%2F0121-0124%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

山田壮亮, 谷本昭英, 島尻正平, 笹栗靖之 . Neurolymphomatosisを合併したdiffuse large B cell lymphomaの1症例 . 福岡大学医学紀要35 ( 1 ) 71 - 72 2008.3

Akihide Tanimoto, Yoshitaka Murata, Ke-Yong Wang, Masato Tsutsui, Kimitoshi Kohno, Yasuyuki Sasaguri . Monocyte chemoattractant protein-1 expression is enhanced by granulocyte-macrophage colony-stimulating factor via jak2-stat5 signaling and inhibited by atorvastatin in human monocytic U937 cells . JOURNAL OF BIOLOGICAL CHEMISTRY283 ( 8 ) 4643 - 4651 2008.2Reviewed International journal

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

The proinflammatory cytokine granulocyte-macrophage colonystimulating factor (GM-CSF) is expressed in inflammatory and atherosclerotic lesions. GM-CSF is known to enhance monocytic expression of monocyte chemoattractant protein-1 (MCP-1). However, the molecular mechanism(s) by which GMCSF up-regulates the MCP-1 expression remains to be clarified. Thus, in this study, we examined our hypothesis that GM-CSF up-regulates the MCP-1 expression via Jak2-Stat5 signaling pathway. In human monocytic cell line U937, GM-CSF increased MCP-1 expression in protein and mRNA levels. Furthermore, analysis of the GM-CSF promoter element revealed that the STAT5 (signal transducer and activator of transcription5) transcription factor binding site, located between-152 and -144 upstream of the transcription start site, as well as Janus kinase-2-mediated Stat5 activation were necessary for the GM-CSF-induced transcriptional up-regulation of the MCP-1 gene. This GM-CSF-induced MCP-1 expression, measured as both protein and mRNA levels, was down-regulated by atorvastatin, a 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor. However, this decrease in MCP-1 expression was not at the transcriptional level of MCP-1 gene but rather at the level of the stability of MCP-1 mRNA. These results indicate that GM-CSF regulates MCP-1 expression via Janus kinase-2-Stat5 pathway and by a novel regulatory mechanism of statins to reduce inflammatory reactions by down-regulating the expression of monocytic MCP-1, which promotes atherogenesis.

DOI： 10.1074/jbc.M708853200

Fumihiko Kamezaki, Hiromi Tasaki, Kazuhito Yamashita, Masato Tsutsui, Shinichiro Koide, Sei Nakata, Akihide Tanimoto, Masahiro Okazaki, Yasuyuki Sasaguri, Tetsuo Adachi, Yutaka Otsuji . Gene transfer of extracellular superoxide dismutase ameliorates pulmonary hypertension in rats . AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE177 ( 2 ) 219 - 226 2008.1Reviewed International journal

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER THORACIC SOC

Rationale: Pulmonary hypertension (PH) is a life-threatening disease, characterized by vascular remodeling and vasoconstriction. Evidence suggests that oxidative stress may contribute to the pathogenesis and/or development of PH.
Objectives: In the present study, we examined whether intratracheal gene transfer of human extracellular superoxide dismutase (EC-SOD) could ameliorate monocrotaline (MCT)-induced PH in rats.
Methods: MCT-injected rats were intratracheally administered vehicle (MCT group) or an adenovirus encoding beta-galactosidase (Ad beta gal group) or human EC-SOD (AdEC-SOD group).
Measurements and Main Results: After intratracheal gene transfer, EC-SOD was successfully expressed in lung tissue, bronchoalveolar lavage fluid, and plasma. Twenty-eight days after MCT injection, right ventricular systolic pressure and the weight ratio of the right ventricle to the left ventricle plus septum were significantly lower in the AdEC-SOD group (42.50 +/- 1.46 mm Hg and 0.453 +/- 0.029, respectively) than in the MCT group (59.89 +/- 1.61 mm Hg and 0.636 +/- 0.022, respectively) or the Ad beta gal group (61.50 +/- 2.61 mm Hg and 0.653 +/- 0.038, respectively). Moreover, vascular remodeling and proliferation of vascular smooth muscle cells in pulmonary arteries were markedly suppressed in the AdEC-SOD group. Importantly, 8-isoprostane in lung tissue was also significantly reduced in the AdEC-SOD group.
Conclusions: EC-SOD overexpression to the lung ameliorated MCT-induced PH in rats. We suggest that EC-SOD may act as an antioxidant in PH and that increased oxidative stress may be important in the pathogenesis of MCT-induced PH.

DOI： 10.1164/rccm.200702-264OC

Wu L, Tanimoto A, Murata Y, Sasaguri T, Fan J, Sasaguri Y, Watanabe T . Matrix metalloproteinase-12 gene expression in human vascular smooth muscle cells. . Genes to Cells.8 ( 3 ) 225 - 234 2008Reviewed

Masaji Hashimoto, Akihide Tanimoto, Tomoharu Yoshiya, Syuichiro Matoba, Toshihito Sawada, Goro Watanabe, Kunihiko Seki, Hiroshi Hashimoto . Mesenchymal tumor of the pancreas: Report of a case . SURGERY TODAY37 ( 12 ) 1117 - 1119 2007.12Reviewed

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Mesenchymal tumors of the pancreas are rare and difficult to classify. We report a case of a solitary mesenchymal tumor in the body of the pancreas. The tumor showed immunocytochemical reactivity for anti-vimentin, anti-alpha-smooth muscle actin, and anti-CD34; however, it was negative immunohistochemically for antibodies to cytokeratins, as well as for the following antibodies: anti-desmin, anti-S-100, anti-chromogranin A, anti-CD117 (c-Kit), anti-CD99, and anti-bcl-2. This tumor could not be classified as a specific type of mesenchymal tumor immunocytochemically.

DOI： 10.1007/s00595-007-3531-7

渡辺次郎, 佐藤圭, 松本聡美, 中島章, 大蔵尚文, 谷本昭英 . Placental site trophoblastic tumorとの鑑別が困難であった子宮動静脈瘻を伴った胎盤遺残と思われる1例 . 診断病理24 ( 4 ) 445 - 447 2007.10

Publisher：(一社)日本病理学会

今回、Placental site trophoblastic tumor(以下PSTT)との鑑別に苦慮した、動静脈瘻を伴う胎盤遺残と思われる1例を経験した。本症例は当初、高度の筋層浸潤、静脈侵襲を伴う中間型トロホブラスト主体の病変であることからPSTTを疑った。しかしトロホブラストの局在が散在性であり、腫瘍としては腫瘤形成性に乏しい点から最終的には胎盤遺残と判断した。(著者抄録)

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Akihide Tanimoto, Ke-Yong Wang, Yoshitaka Murata, Satoshi Kimura, Masako Nomaguchi, Sei Nakata, Masato Tsutsui, Yasuyuki Sasaguri . Histamine upregulates the expression of inducible nitric oxide synthase in human intimal smooth muscle cells via histamine H1 receptor and NF-kappa B signaling pathway . ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY27 ( 7 ) 1556 - 1561 2007.7Reviewed International journal

Language：English Publishing type：Research paper (scientific journal) Publisher：LIPPINCOTT WILLIAMS & WILKINS

Objective - Histamine increases endothelial nitric oxide ( NO) production as an endothelium- dependent vasodilator, which acts as a vasoconstrictor in atherosclerotic coronary arteries. To investigate the relation between histamine and NO production in intimal smooth muscle cells ( SMCs), we studied the effect of histamine on inducible NO synthase ( iNOS) expression in the SMCs.
Methods and Results - In cultured human intimal SMCs, histamine increased NO production, iNOS expression, and NF-kappa B nuclear translocation, which were inhibited by histamine H1 blocker and NF-kappa B inhibitor. Luciferase assay using -8.3 kb upstream of human iNOS promoter region and electrophoretic mobility shift assay suggested that a NF-kappa B motif located at -3922 to -3914 would be necessary for histamine- inducible promoter activity. In addition, H1 blocker, NF-kappa B inhibitor, and dominant negative I kappa B alpha or I kappa B kinase beta downregulated the histamine- induced iNOS promoter activity. In the human aorta, histamine content was estimated to be 310 +/- 66 pmol/ mg protein in the atherosclerotic intima, while that was to be 43 +/- 22 pmol/ mg protein in the media ( P < 0.001).
Conclusions - Histamine stimulates intimal SMCs to increase iNOS expression via H1 receptors and NF-kappa B signaling pathway. Histamine could be one of NO- regulating factors, by inducing iNOS expression in intimal SMCs, and may be related to atherogenesis.

DOI： 10.1161/ATVBAHA.106.139089

山田壮亮, 谷本昭英, 松木康真, 島尻正平, 笹栗靖之 . Pancreatic hamartomaの1症例 . 診断病理24 ( 3 ) 335 - 338 2007.7

Publisher：(一社)日本病理学会

71歳女性の膵体尾部に発生した過誤腫の1例を経験したので報告する。2年前、上行結腸癌にて切除術を施行され、経過観察中の腹部CTにて膵尾部嚢胞性腫瘍が認められた。悪性を疑われ、膵体尾部脾合併切除術を施行された。腫瘍は径5×4×3cmの大きさで膵体尾部に限局する境界明瞭な白色調の病変であり、内部に出血と嚢胞性変化を伴っていた。組織学的に、充実性成分から嚢胞性成分への細胞密度の移行は緩やかであり、後者では上皮の裏打ちを認めなかった。充実性部分では、紡錘形から類円形の腫瘍細胞が無秩序に増殖しており、一部では硝子化した厚みのある線維性間質を伴っていた。鑑別として孤在性線維性腫瘍と胃腸間質性腫瘍が考えられたが、ラ氏島形成がなく、腺房細胞と介在導管上皮細胞の配置および構成割合に異常の認められる腺房組織を内在していたことより過誤腫と診断した。(著者抄録)

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Shinichiro Koide, Masahiro Okazaki, Masahito Tamura, Kiyoshi Ozumi, Hiroyuki Takatsu, Fumihiko Kamezaki, Akihide Tanimoto, Hiromi Tasaki, Yasuyuki Sasaguri, Yasuhide Nakashima, Yutaka Otsuji . PTEN reduces cuff-induced neointima formation and proinflammatory cytokines . AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY292 ( 6 ) H2824 - H2831 2007.6Reviewed International journal

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER PHYSIOLOGICAL SOC

An inflammatory response followed by vascular injury plays an important role in neointima formation and development of atherosclerotic lesions, which are in part mediated by proinflammatory cytokines. Using a Cuff injury model, we examined the effects of adenovirus-mediated overexpression of phosphatase and tensin homology deleted on chromosome 10 (PTEN) on neointima formation and the proinflammatory response. A cuff was placed around the femoral artery. and adenovirus expressing human PTEN type 1 (AdPTEN) or Escherichia coli P-galactosidase (AdLacZ) was injected between the cuff and the adventitia. After 14 days, the arteries were examined histopathologically and by Western blotting. The significant reduction of neointima formation by AdPTEN compared with AdLacZ was accompanied by reduced cell proliferation and increased adventitial cell apoptosis. AdPTEN also reduced expression of phosphorylated I kappa B-alpha., but not nonphosphorylated I kappa B-alpha. Western blotting revealed that AdPTEN reduced the cuff injury-induced expression levels of monocyte chemoattractant protein-1, TNF-alpha, and IL-1 beta and their expression in all layers of the arterial wall. In contrast, cuff-induced macrophage invasion, which was also inhibited by AdPTEN, was detected only at the intimal Surface and in the adventitia. In cultured vascular smooth muscle cells, PTEN directly inhibited ANG II-induced monocyte chemoattractant protein-1 expression as quantified by real-time PCR and Western blotting. Our results suggest that overexpression of PTEN reduces neointima formation, possibly in part through inhibition of the inflammatory response by macrophage invasion and proinflammatory cytokine expression.

DOI： 10.1152/ajpheart.01221.2006

堤謙二, 宇田川晴司, 木ノ下義宏, 上野正紀, 峯真司, 江原一尚, 谷本昭英, 鶴丸昌彦 . リンパ節微小転移からみた早期胃癌における色素法によるセンチネルリンパ節/リンパ流域の検討 . 日本臨床外科学会雑誌68 ( 3 ) 540 - 546 2007.3

Publisher：日本臨床外科学会

色素法によるセンチネルリンパ節(sentinel node;SN)を指標としたリンパ節転移診断の問題点を微小転移の観点から検討した.早期胃癌57例を対象に,色素法によるSNおよびセンチネルリンパ流(Sentinel lymphatic basin,SB)同定率,SNおよびSBを指標とした微小転移を含む転移検出感度,正診率を検討した.微小転移は癌細胞1個をtumor cell microinvolvement,cluster形成をtumor clusterと定義した.M癌では2.9%(1/34),SM癌では60.9%(14/23)に微小転移を認めた.微小転移を指標としたSN同定率は100%,感度60.0%,正診率89.5%と感度が低く,一方SB単位で検討すると感度は86.7%,正診率96.5%と良好であった.早期胃癌におけるリンパ節微小転移頻度は高く,SNを指標とした場合の偽陰性症例の頻度を考慮すると,現状ではSNを指標とするよりもSBを指標とした方がより安全性の高い縮小手術が可能であると考えられた.(著者抄録)

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2007&ichushi_jid=J03156&link_issn=&doc_id=20070330020003&doc_link_id=10.3919%2Fjjsa.68.540&url=https%3A%2F%2Fdoi.org%2F10.3919%2Fjjsa.68.540&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

Sei Nakata, Masato Tsutsui, Hiroaki Shimokawa, Takahiro Yamashita, Akihide Tanimoto, Hiromi Tasaki, Kiyoshi Ozumi, Ken Sabanai, Tsuyoshi Morishita, Osamu Suda, Hideyasu Hirano, Yasuyuki Sasaguri, Yasuhide Nakashima, Nobuyuki Yanagihara . Statin treatment upregulates vascular neuronal nitric oxide synthase through Akt/NF-kappa B pathway . ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY27 ( 1 ) 92 - 98 2007.1Reviewed International journal

Language：English Publishing type：Research paper (scientific journal) Publisher：LIPPINCOTT WILLIAMS & WILKINS

Objective - Three-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) are known to enhance vascular expression of endothelial (eNOS) and inducible nitric oxide synthase (iNOS). In this study, we examined whether statins also upregulate vascular expression of neuronal NOS (nNOS).
Methods and Results - In cultured rat aortic smooth muscle cells, treatment with atorvastatin significantly increased nNOS expression, associated with activation of Akt and NF-kappa B. Inhibition of Akt by dominant-negative Akt suppressed atorvastatin-induced nNOS expression as well as Akt and NF-kappa B activation. Inhibition of NF-kappa B by dominant-negative I kappa B also attenuated atorvastatin-induced nNOS expression and NF-kappa B activation, but not Akt activation. We further examined whether atorvastatin also enhances nNOS expression in isolated mouse aorta, and if so, how much nNOS-derived NO accounts for atorvastatin-induced NOx production. In isolated aortas of wild-type mice, atorvastatin significantly increased all three NOS isoform expression and NOx production. In isolated aortas of doubly i/eNOS(-/-), n/eNOS(-/-), and n/iNOS(-/-) mice, which express only nNOS, iNOS, and eNOS, respectively, atorvastatin- induced NOx production was approximately 25%, 25%, and 50% to that of wild-type mice, respectively, suggesting that nNOS accounts for 25% of the atorvastatin-mediated NOx production.
Conclusions - These results indicate that atorvastatin upregulates vascular nNOS through Akt/NF-kappa B pathway, demonstrating a novel nNOS-mediated vascular effect of the statin.

DOI： 10.1161/01.ATV.0000251615.61858.33

山田壮亮, 谷本昭英, 笹栗毅和, 松木康真, 島尻正平, 笹栗靖之 . 子宮頸部に見られたendocervicosisの1症例 . 診断病理24 ( 1 ) 102 - 105 2007.1

Publisher：(一社)日本病理学会

47歳女性の子宮頸部に発生し、腟腔を占拠するendocervicosisの1例を経験したので報告する。約2年前より徐々に増大する下腹部膨隆が出現、卵巣嚢腫と診断され経過観察されていた。1ヵ月前より悪臭の強い帯下を認めたが精査出来ず、単純子宮全摘出術が施行された。病変は子宮腟部・後唇から突出する12×9×7cmの柔らかい腫瘤であり、腟腔を占拠していた。割面では粘液の貯留した多房状嚢胞性病変であった。腫瘤は大小の嚢胞性腺管により構成されており、異型のない内頸部型の粘液性円柱上皮が一層性に並んでいた。鑑別としてminimal deviation adenocarcinoma(adenoma malignum)とdeep nabothian cystが考えられたが、正常頸管腺との連続性がないこと、腺管の嚢胞性変化が見られること、および細胞異型が乏しいことなどよりendocervicosisと診断した。(著者抄録)

Akihide Tanimoto, Yasuyuki Sasaguri, Hiroshi Ohtsu . Histamine network in atherosclerosis . TRENDS IN CARDIOVASCULAR MEDICINE16 ( 8 ) 280 - 284 2006.11Reviewed International journal

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE LONDON

Histamine is a low-molecular-weight amine, synthesized from L-histidine by histidine decarboxylase. It has been suggested that the histamine is produced in the atherosclerotic lesion although the activity of histamine has not been clarified completely. To avoid the pharmacologic problems, genetically engineered mice are useful. We recently observed the histidine decarboxylase-gene knockout mice ameliorates' atherosclerotic region, compared with that of the wild-type control mice. The source of histamine in atherosclerotic lesion should be clarified in details; however, it could be macrophage, endothelial cells, and mast cells. All four types of histamine receptors (H1-H4) have the possibilities to be involved in the atherosclerotic regions. Because H1 and H2 receptors are discovered previously, the activities through those receptors are investigated relatively well, but as the other two types of receptors have been cloned recently, their involvement in atherosclerotic lesion should be investigated further.

DOI： 10.1016/j.tcm.2006.06.001

山田壮亮, 谷本昭英, 松木康真, 笹栗靖之 . マンソン孤虫症Sparganosisの1症例 . 診断病理23 ( 4 ) 296 - 298 2006.10

Publisher：(一社)日本病理学会

70歳女性の右乳房皮下に認められたマンソン孤虫症の1例を経験したので報告する。偶然右乳房B領域に硬結を指摘され、局麻下に腫瘤切除術を施行された。腫瘤性病変の主体は蛇行する虫体であり、周囲には肉芽腫の形成と好酸球の浸潤が認められた。虫体には、外側を覆う好酸性の外被、その内層に縦列する紡錘形の外被細胞、および疎な内部組織が認められた。内部組織には腸管などの分化した器官は見られなかったが、同心円状の石灰小体が分布していた。以上より、マンソン裂頭条虫の幼虫であるPlerocercoid(擬充尾虫)がヒトを第二中間宿主として寄生し迷入・迷走した皮膚爬行症creeping diaseseと考え、マンソン孤虫症Sparganosisと診断した。(著者抄録)

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2006&ichushi_jid=J03623&link_issn=&doc_id=20061107260012&doc_link_id=%2Fcd9jjodp%2F2006%2F002304%2F012%2F0296-0298%26dl%3D0&url=http%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcd9jjodp%2F2006%2F002304%2F012%2F0296-0298%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

山田壮亮, 松木康真, 谷本昭英, 笹栗毅和, 中野盛夫, 笹栗靖之 . Aggressive digital papillary adenocarcinomaの1症例 . 診断病理23 ( 3 ) 193 - 195 2006.7

Publisher：(一社)日本病理学会

79歳男性の右拇指腹側に発生したaggressive digital papillary adenocarcinoma(以下ADPAca)の1例を経験したので報告する.右拇指腹側に,約3ヵ月の間に徐々に増大する径20〜30mm大の皮下腫瘤を認め,切開掻爬された.腫瘍は境界明瞭で,分葉状に充実性発育しており,内部に管状・低乳頭状構造を伴っていた.腫瘍細胞は円形から紡錘形で,核の大小不同,核分裂像の認められる異型上皮細胞であり,免疫組織化学的にAE1/AE3,EMAおよびS-100に陽性を呈した.Chondroid syringomaとの鑑別が考えられたが,細胞異型と特徴的な増殖様式よりADPAcaと診断した(著者抄録)

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Y Kohno, Y Matsuki, A Tanimoto, H Izumi, T Uchiumi, K Kohno, S Shimajiri, Y Sasaguri . Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen . BRITISH JOURNAL OF CANCER94 ( 5 ) 710 - 716 2006.3Reviewed

Language：English Publishing type：Research paper (scientific journal) Publisher：NATURE PUBLISHING GROUP

Y- box- binding proteins are members of the human cold- shock domain protein superfamily, which includes dbpA, dbpB/ YB- 1, and dbpC/ contrin. dbpC/ contrin is a germ cell- specific Y- box- binding protein and is suggested to function as a nuclear transcription factor and RNA- binding protein in the cytoplasm. Whereas ubiquitous dbpB/ YB- 1 expression has been well studied in various types of human carcinomas as a prognostic or predictive marker, the dbpC/ contrin expression in human tumour cells has not been reported. In this report, we provide the first evidence showing that dbpC was highly expressed in human testicular seminoma and ovarian dysgerminomas, and in carcinomas in other tissues and that its expression in normal tissues is nearly restricted to germ cells and placental trophoblasts. These results indicate that dbpC/ contrin would be a potentially novel cancer/ testis antigen.

DOI： 10.1038/sj.bjc.6602987

堤謙二, 宇田川晴司, 木ノ下義宏, 上野正紀, 峯真司, 江原一尚, 谷本昭英, 鶴丸昌彦 . 早期胃癌に対する幽門輪温存手術の適応からみた幽門上リンパ節微小転移の臨床的意義 . 日本臨床外科学会雑誌67 ( 2 ) 281 - 287 2006.2

Publisher：日本臨床外科学会

早期胃癌における幽門上リンパ節(以下No. 5)への癌微小転移状況から,迷走神経幽門枝/右胃動脈を温存した幽門保存胃切除術(PPG)の適応拡大の可能性について検討した.D2郭清を行った中下部早期胃癌267例を対象にCytokeratin(CAM5.2)染色,HE染色によるNo. 5転移状況を検討.癌細胞が1個のtumor cell micro involvement(MI)が6例,cluster形成したmicro metastasis(MM)が2例,HE染色での転移は1例.M癌ではMIが1例.SM1癌では転移を認めず,SM2癌ではMIが5例,MMが2例,HE染色で1例,SM2癌は全体の9.3%(8/86)に転移を認めた.さらにSM2の小彎病変では16.7%(5/30),非小彎病変5.4%(3/56),組織型は全例,中・低分化型で,中低分化型・小彎病変の19.2%(5/26)に転移を認めた.微小転移の臨床的意義はいまだ解明されていないが,SM2癌でのPPG適応の際には慎重を期す必要がある

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2006&ichushi_jid=J03156&link_issn=&doc_id=20060228340003&doc_link_id=10.3919%2Fjjsa.67.281&url=https%3A%2F%2Fdoi.org%2F10.3919%2Fjjsa.67.281&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

Yoshida T, Izumi H, Uchiumi T, Sasaguri Y, Tanimoto A, Matsumoto T, Naito S, Kohno K . Expression and cellular localization of dbpC/Contrin in germ cell tumor cell lines. . Biochimica et biophysica acta1759 ( 1-2 ) 80 - 88 2006.1

T Yoshida, H Izumi, T Uchiumi, Y Sasaguri, A TaniMoto, T Matsumoto, S Naito, K Kohno . Expression and cellular localization. of dbpC/Contrin in germ cell tumor cell lines . BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION1759 ( 1-2 ) 80 - 88 2006.1

Publisher：ELSEVIER SCIENCE BV

The transcriptional regulation of the germ cell-specific cold-shock domain protein dbpC/Contrin was investigated, and the promoter region between -272 and -253 relative to the transcription start site was shown to be critical for the manifestation of cell-type specific transcription. In vivo footprint analysis demonstrated that the E-box located between -272 and -253 is protected in the dbpC/Contrin-positive germ cell tumor cell lines NEC8 and TERA1, but not in the dbpC/Contrin-negative bladder cancer cell line T24 or ovarian cancer cell line A2780. The promoter activity of the dbpC/Contrin gene was transactivated by co-transfection with c-Myc and the N-Myc expression plasmid. Western blotting analysis clearly showed that N-Myc is highly expressed in both NEC8 and TERA1 cells, and that c-Myc is expressed in both T24 and A2780 cells. These data demonstrate that cell-type specific dbpC/Contrin expression in germ cells is regulated by N-Myc. In addition, dbpC/Contrin is localized mainly in the cytoplasm of NEC8 and TERA1 cells, but is translocated to the nucleus when its C-terminal region is partially deleted. Our findings also suggest that dbpC/Contrin can be used as a molecular tool for the detection of germ cell tumors. (c) 2006 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.bbaexp.2006.02.005

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