研究者詳細 - 小原　恭子

論文 - 小原　恭子

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Kayesh M.E.H. . Toll-like receptor response to hepatitis Bb virus infection and potential of TLR agonists as immunomodulators for treating chronic hepatitis b: An overview . International Journal of Molecular Sciences22 ( 19 ) 2021年9月査読国際誌

Abbadi I. . Non-primate hepacivirus transmission and prevalence: Novel findings of virus circulation in horses and dogs in Morocco . Infection, Genetics and Evolution93 104975 - 104975 2021年9月査読国際誌

ケアシュ・モハメド, 尼子豊, ハシェム・モハメド, 山本直樹, 杉山真也, 溝上雅史, 小原道法, 小原恭子 . B型肝炎ウイルスcccDNAを標的としたCRISPR/Cas9システムの生体内送達法の開発 . 日本癌学会総会記事80回 [J3 - 5] 2021年9月査読国際誌

Honda T, Gomi S, Yamane D, Yasui F, Yamamoto T, Munakata T, Itoh Y, Ogasawara K, Sanada T, Yamaji K, Yasutomi Y, Tsukiyama-Kohara K, Kohara M. . Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA . mSphere6 ( 3 ) e1342-20 2021年5月査読

Honda T. . Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies . mSphere6 ( 3 ) 1 - 14 2021年5月査読国際誌

Saito M. . Macrocyclic peptides exhibit antiviral effects against influenza virus HA and prevent pneumonia in animal models . Nature Communications12 ( 1 ) 2654 - 2654 2021年5月査読国際誌

Toll-Like Receptor and Cytokine Responses to Infection with Endogenous and Exogenous Koala Retrovirus, and Vaccination as a Control Strategy . Curr Issues Mol Biol. 43 ( 1 ) 52 - 64 2021年4月査読国際共著

Md Abul Hashem1, 2, 3, Mohammad Enamul Hoque Kayesh1, 2, 4, Fumie Maetani5, Taiki Eiei5, Kyoya Mochizuki5, Shinsaku Ochiai5, Ayaka Ito5, Nanao Ito5, Hiroko Sakurai5, Takayuki Asai5 and Kyoko Tsukiyama-Kohara . Koala retrovirus (KoRV) subtypes and their impact on captive koala (Phascolarctos cinereus) health . Archives of Virologyaccepted 2021年4月査読

Mohammad Enamul Hoque Kayesh 1,2 , Md Abul Hashem 1,3,4 and Kyoko Tsukiyama-Kohara . Toll-Like Receptor Expression Profiles in Koala (Phascolarctos cinereus) Peripheral Blood Mononuclear Cells Infected with Multiple KoRV Subtypes . 11 983 2021年4月査読

Kayesh M.E.H., Hashem M.A., Tsukiyama-Kohara K. . Toll-like receptor expression profiles in koala (Phascolarctos cinereus) peripheral blood mononuclear cells infected with multiple korv subtypes . Animals11 ( 4 ) 2021年4月査読国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Animals

Toll-like receptors (TLRs), evolutionarily conserved pattern recognition receptors, play an important role in innate immunity by recognizing microbial pathogen-associated molecular patterns. Koala retrovirus (KoRV), a major koala pathogen, exists in both endogenous (KoRV-A) and exogenous forms (KoRV-B to J). However, the expression profile of TLRs in koalas infected with KoRV-A and other subtypes is yet to characterize. Here, we investigated TLR expression profiles in koalas with a range of subtype infection profiles (KoRV-A only vs. KoRV-A with KoRV-B and/or-C). To this end, we cloned partial sequences for TLRs (TLR2–10 and TLR13), developed real-time PCR assays, and determined TLRs mRNA expression patterns in koala PBMCs and/or tissues. All the reported TLRs for koala were expressed in PBMCs, and variations in TLR expression were observed in koalas infected with exogenous subtypes (KoRV-B and KoRV-C) compared to the endogenous subtype (KoRV-A) only, which indicates the implications of TLRs in KoRV infection. TLRs were also found to be differentially expressed in koala tissues. This is the first report of TLR expression profiles in koala, which provides insights into koala’s immune response to KoRV infection that could be utilized for the future exploitation of TLR modulators in the maintenance of koala health.

DOI： 10.3390/ani11040983

Scopus

PubMed

Nagai M, Okabayashi T, Akagami M, Matsuu A, Fujimoto Y, Hashem MA, Mekata H, Nakao R, Matsuno K, Katayama Y, Oba M, Omatsu T, Asai T, Nakagawa K, Ito H, Madarame H, Kawai K, Ito T, Nonaka N, Tsukiyama-Kohara K, Inoshima Y, Mizutani T, Misawa N. . Metagenomic identification, sequencing, and genome analysis of porcine hepe-astroviruses (bastroviruses) in porcine feces in Japan . Infect Genet Evol.88 104664 2021年3月査読

Nagai M., Okabayashi T., Akagami M., Matsuu A., Fujimoto Y., Hashem M.A., Mekata H., Nakao R., Matsuno K., Katayama Y., Oba M., Omatsu T., Asai T., Nakagawa K., Ito H., Madarame H., Kawai K., Ito T., Nonaka N., Tsukiyama-Kohara K., Inoshima Y., Mizutani T., Misawa N. . Metagenomic identification, sequencing, and genome analysis of porcine hepe-astroviruses (bastroviruses) in porcine feces in Japan . Infection, Genetics and Evolution88 104664 - 104664 2021年3月査読国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Infection, Genetics and Evolution

Recently, hepe-astrovirus-like RNA viruses named bastroviruses (BastVs), have been found in human, pig, bat, and rat fecal samples. In this study, we determined nearly complete genome sequences of four BastVs in the feces of healthy pigs. Genetic characterization revealed that these porcine BastVs (PBastVs) and BastVs from other animals including humans, had the same genome organization, that is, they contained three predicted conserved domains of viral methyltransferase, RNA helicase, and RdRp in the nonstructural ORF1 and the astrovirus capsid domain in the structural ORF2. Phylogenetic analyses using RNA-dependent RNA polymerase and the capsid region revealed that PBastVs branched with bat and rat BastVs; however, the groups formed by each host were distantly related to human BastVs. Pairwise amino acid sequence comparison demonstrated that PBastVs shared 95.2–98.6% and 76.1–95.5% sequence identity among each other in the ORF1 and ORF2 regions, respectively; the sequence identities between PBastVs and BastVs from other animals were 21.4–42.5% and 9.1–20.6% in the ORF1 and ORF2 regions, respectively. This suggested that BastVs were derived from a common ancestor but evolved independently in each host population during a prolonged period. Putative recombination events were identified in the PBastV genome, suggesting that PBastVs gain sequence diversity and flexibility through recombination events. In an analysis of previously obtained metagenomic data, PBastV sequence reads were detected in 7.3% (23/315) of fecal samples from pigs indicating that PBastVs are distributed among pig populations in Japan.

DOI： 10.1016/j.meegid.2020.104664

Scopus

PubMed

Suzuki Y, Onuma H, Sato R, Sato Y, Hashiba A, Maeki M, Tokeshi M, Kayesh MEH, Kohara M, Tsukiyama-Kohara K, Harashima H. . Lipid nanoparticles loaded with ribonucleoprotein-oligonucleotide complexes synthesized using a microfluidic device exhibit robust genome editing and hepatitis B virus inhibition. . 330 67 - 71 2021年2月査読

Suzuki Y. . Lipid nanoparticles loaded with ribonucleoprotein–oligonucleotide complexes synthesized using a microfluidic device exhibit robust genome editing and hepatitis B virus inhibition . Journal of Controlled Release330 61 - 71 2021年2月査読国際誌

Kayesh M.E.H. . Recent Insights Into the Molecular Mechanism of Toll-Like Receptor Response to Dengue Virus Infection . Frontiers in Microbiology12 744233 - 744233 2021年査読国際誌

CD4, CD8b, and Cytokines Expression Profiles in Peripheral Blood Mononuclear Cells Infected with Different Subtypes of KoRV from Koalas (Phascolarctos cinereus) in a Japanese Zoo. . Viruses12 ( 12 ) 1415 2020年12月

Kayesh M.E.H. . Cd4, cd8b, and cytokines expression profiles in peripheral blood mononuclear cells infected with different subtypes of korv from koalas (Phascolarctos cinereus) in a japanese zoo . Viruses12 ( 12 ) 2020年12月査読国際誌

Ezzikouri S, Nourlil J, Benjelloun S, Kohara M, Tsukiyama-Kohara K. . Coronavirus disease 2019-Historical context, virology, pathogenesis, immunotherapy, and vaccine development. . Hum Vaccin Immunother. 16 ( 12 ) 2992 - 3000 2020年12月招待査読

Kayesh MEH, Amako Y, Hashem MA, Murakami S, Ogawa S, Yamamoto N, Hifumi T, Miyoshi N, Sugiyama M, Tanaka Y, Mizokami M, Kohara M, Tsukiyama-Kohara K. . Development of an in vivo delivery system for CRISPR/Cas9-mediated targeting of hepatitis B virus cccDNA . Virus Research290 198191 2020年12月査読

Ezzikouri S. . Coronavirus disease 2019—Historical context, virology, pathogenesis, immunotherapy, and vaccine development . Human Vaccines and Immunotherapeutics16 ( 12 ) 2992 - 3000 2020年12月査読国際誌

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