研究者詳細 - 小原　恭子

論文 - 小原　恭子

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Soliman AM, Mahmoud HYAH, Amer MM, Mohamed S, Hifumi T, Tsukiyama-Kohara K, Tanaka T . First detection of Rickettsia japonica in Hyalomma dromedarii ticks infesting camels from Egypt: A call for enhanced surveillance of tick-borne pathogens. . Medical and veterinary entomology 2025年9月査読国際誌

Akter L, Hashem MA, Kayesh MEH, Rakib TM, Rashid MHO, Maetani F, Tsukiyama-Kohara K . Correction: Elevated oncogene expressions in koala infected with multiple koala retrovirus subtypes: a preliminary study. . Virus genes 2025年8月査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Toll-like Receptor (TLR) Response in Chikungunya Virus Infection: Mechanism of Activation, Immune Evasion, and Use of TLR Agonists in Vaccine Development. . Vaccines13 ( 8 ) 2025年8月査読国際誌

Kayesh M.E.H., Kohara M., Tsukiyama-Kohara K. . Innate Immune Response to Powassan Virus Infection: Progress Toward Infection Control . Vaccines13 ( 7 ) 2025年7月査読国際誌

担当区分：筆頭著者,　最終著者,　責任著者記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Vaccines

Powassan virus is an emerging tick-borne flavivirus that poses a significant threat to human health. The outcome of Powassan virus infection is shaped by both viral factors and the host immune response. While this review aimed to examine the innate immune response, particularly toll-like receptor-mediated immune responses to Powassan virus, data specific to the immune response to Powassan virus remain scarce. Therefore, we focused on toll-like receptor responses to related flaviviruses to infer possible mechanisms of host response. Insights from both in vivo and in vitro studies are critical for guiding the development of effective therapeutic and preventive strategies. Currently, there are no clinically approved treatments or vaccines for Powassan virus, highlighting the urgent need for their development. We also highlight recent progress in POWV vaccine development, with an emphasis on the potential use of toll-like receptor agonists as adjuvants to enhance immunogenicity and improve vaccine efficacy.

DOI： 10.3390/vaccines13070754

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Akter L, Hashem MA, Kayesh MEH, Rakib TM, Rashid MHO, Maetani F, Kohara KT . Elevated oncogene expressions in koala infected with multiple koala retrovirus subtypes: a preliminary study. . Virus genes 2025年6月査読国際誌

Sanada T, Tsukiyama-Kohara K, Kohara M . Genomic characterization of hepaciviruses and pegivirus in the northern tree shrew (Tupaia belangeri). . Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 105778 2025年6月査読国際誌

Rashid MHO, Ezzikouri S, Soliman AM, Akter L, Momohara K, Hifumi T, Miyoshi N, Hishiki T, Abdel-Moneim AS, Kohara M, Tsukiyama-Kohara K . Drug repositioning: Identification of potent inhibitors of NS3 protease and NS5 RdRp for control of DENV infection. . Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie187 118104 2025年4月査読国際誌

Rashid MHO, Yasui F, Sanada T, Kono R, Honda T, Kitab B, Akter L, Utsunomiya M, Sato R, Yoshida O, Hiasa Y, Oda Y, Goh Y, Miyazaki T, Kohara M, Tsukiyama-Kohara K . Immunogenicity of an Intranasal Dual (Core and Surface)-Antigen Vaccine Against Hepatitis B Virus Enhanced by Carboxyl-Vinyl Polymer Excipients. . Vaccines13 ( 5 ) 2025年4月査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Effects of oxidative stress on viral infections: an overview. . Npj viruses3 ( 1 ) 27 2025年4月査読国際誌

Akhter R, Hossain KA, Kitab B, Sakoda Y, Tsukiyama-Kohara K . Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses. . Virus research 199570 2025年4月査読国際誌

Uno Y, Kawabata I, Ushirozako G, Tsukiyama-Kohara K, Ishizuka M, Mizukawa H, Murayama N, Yamazaki H . Exploration of functional cytochrome P450 4F enzymes in liver, intestine, and kidney from dogs, cats, pigs, and tree shrews and comparison of their metabolic capacities with human P450 4F2 and 4F12. . Biochemical pharmacology 116894 2025年3月査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Toll-like receptor response to Zika virus infection: progress toward infection control. . Npj viruses3 ( 1 ) 20 2025年3月査読国際誌

Rashid MHO, Kayesh MEH, Hashem MA, Hifumi T, Ogawa S, Miyoshi N, Tanaka Y, Kohara M, Tsukiyama-Kohara K . Adeno-associated virus 2 CRISPR/Cas9-mediated targeting of hepatitis B virus in tree shrews. . Virus research 199550 2025年2月査読国際誌

Hifumi T., Tanaka Y., Tsukiyama-Kohara K., Miyoshi N. . Phyllodes tumor in the mammary gland of a northern tree shrew (Tupaia belangeri) . Journal of Veterinary Medical Science87 ( 4 ) 384 - 388 2025年査読国際誌

担当区分：筆頭著者,　最終著者,　責任著者記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Veterinary Medical Science

A rapidly growing mass was observed in the left third mammary gland of a 5-and-a-half-year-old intact female northern tree shrew (Tupaia belangeri). Histopathologically, the mass was composed of numerous irregularly dilated mammary ducts with a pericanalicular or intracanalicular pattern. These mammary ducts showed a leaf-like structure due to the proliferations of surrounding spindle-shaped neoplastic cells with moderate to severe nuclear atypia. We diagnosed this case as a phyllodes tumor in a mammary gland of a northern tree shrew (Tupaia belangeri), which closely resembled those occurring in the human breast histopathologically. To the best of our knowledge, this is the first report of a phyllodes tumor in the mammary gland of a northern tree shrew (Tupaia belangeri).

DOI： 10.1292/jvms.24-0444

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Soliman AM, Mahmoud HYAH, Amer MM, Mohamed S, Hifumi T, Tsukiyama-Kohara K, Tanaka T . First detection of Colpodella spp. in Rhipicephalus annulatus and molecular characterization of piroplasmids in southern Egypt. . Frontiers in veterinary science12 1617204 2025年査読国際誌

Akter L, Hashem MA, Kayesh MEH, Hossain MA, Maetani F, Akhter R, Hossain KA, Rashid MHO, Sakurai H, Asai T, Hoque MN, Tsukiyama-Kohara K . A preliminary study of gene expression changes in Koalas Infected with Koala Retrovirus (KoRV) and identification of potential biomarkers for KoRV pathogenesis. . BMC veterinary research20 ( 1 ) 496 2024年10月査読国際誌

Kayesh MEH, Hashem MA, Tsukiyama-Kohara K . Epidemiology, Transmission Mode, and Pathogenesis of Chlamydia pecorum Infection in Koalas (Phascolarctos cinereus): An Overview. . Animals : an open access journal from MDPI14 ( 18 ) 2024年9月査読国際誌

Yao YG, Lu L, Ni RJ, Bi R, Chen C, Chen JQ, Fuchs E, Gorbatyuk M, Lei H, Li H, Liu C, Lv LB, Tsukiyama-Kohara K, Kohara M, Perez-Cruz C, Rainer G, Shan BC, Shen F, Tang AZ, Wang J, Xia W, Xia X, Xu L, Yu D, Zhang F, Zheng P, Zheng YT, Zhou J, Zhou JN . Study of tree shrew biology and models: A booming and prosperous field for biomedical research. . Zoological research45 ( 4 ) 877 - 909 2024年7月査読国際誌

Uno Y, Tsukiyama-Kohara K, Ishizuka M, Mizukawa H, Murayama N, Yamazaki H . Investigation of functional cytochrome P450 4A enzymes in liver and kidney of pigs, cats, tree shrews, and dogs in comparison with the metabolic capacity of human P450 4A11. . Drug metabolism and disposition: the biological fate of chemicals 2024年7月査読国際誌

Kayesh M.E.H., Kohara M., Tsukiyama-Kohara K. . Recent Insights into the Molecular Mechanisms of the Toll-like Receptor Response to Influenza Virus Infection . International Journal of Molecular Sciences25 ( 11 ) 2024年6月査読国際誌

担当区分：筆頭著者,　最終著者,　責任著者記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：International Journal of Molecular Sciences

Influenza A viruses (IAVs) pose a significant global threat to human health. A tightly controlled host immune response is critical to avoid any detrimental effects of IAV infection. It is critical to investigate the association between the response of Toll-like receptors (TLRs) and influenza virus. Because TLRs may act as a double-edged sword, a balanced TLR response is critical for the overall benefit of the host. Consequently, a thorough understanding of the TLR response is essential for targeting TLRs as a novel therapeutic and prophylactic intervention. To date, a limited number of studies have assessed TLR and IAV interactions. Therefore, further research on TLR interactions in IAV infection should be conducted to determine their role in host–virus interactions in disease causation or clearance of the virus. Although influenza virus vaccines are available, they have limited efficacy, which should be enhanced to improve their efficacy. In this study, we discuss the current status of our understanding of the TLR response in IAV infection and the strategies adopted by IAVs to avoid TLR-mediated immune surveillance, which may help in devising new therapeutic or preventive strategies. Furthermore, recent advances in the use of TLR agonists as vaccine adjuvants to enhance influenza vaccine efficacy are discussed.

DOI： 10.3390/ijms25115909

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Ushirozako G, Murayama N, Tsukiyama-Kohara K, Yamazaki H, Uno Y . Novel tree shrew cytochrome P450 2Ds ( CYP2D8a and CYP2D8b ) are functional drug-metabolizing enzymes that metabolize bufuralol and dextromethorphan. . Drug metabolism and disposition: the biological fate of chemicals 2024年1月査読国際誌

Uno Y, Makiguchi M, Ushirozako G, Tsukiyama-Kohara K, Shimizu M, Yamazaki H . Molecular and functional characterization of flavin-containing monooxygenases (FMO1-6) in tree shrews. . Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 109835 2024年1月査読国際誌

Kayesh MEH, Nazneen H, Kohara M, Tsukiyama-Kohara K . An effective pan-serotype dengue vaccine and enhanced control strategies could help in reducing the severe dengue burden in Bangladesh-A perspective. . Frontiers in microbiology15 1423044 2024年査読国際誌

Hasan MN, Rahman MM, Husna AA, Arif M, Iwanaga T, Tsukiyama-Kohara K, Jasineviciute I, Kato D, Nakagawa T, Miura N . Elevated expression of miR-301a and its functional roles in canine oral melanoma. . Veterinary and comparative oncology 2023年12月査読国際誌

Uno Y, Minami Y, Tsukiyama-Kohara K, Murayama N, Yamazaki H . Identification of cytochrome P450 2C18 and 2C76 in tree shrews: P450 2C18 effectively oxidizes typical human P450 2C9/2C19 chiral substrates warfarin and omeprazole with less stereoselectivity. . Biochemical pharmacology 115990 2023年12月査読国際誌

Ngwe Tun MM, Nwe KM, Balingit JC, Takamatsu Y, Inoue S, Pandey BD, Urano T, Kohara M, Tsukiyama-Kohara K, Morita K . A Novel, Comprehensive A129 Mouse Model for Investigating Dengue Vaccines and Evaluating Pathogenesis. . Vaccines11 ( 12 ) 2023年12月査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Effects of neddylation on viral infection: an overview. . Archives of virology169 ( 1 ) 6 2023年12月査読国際誌

Akter L, Hashem MA, Rakib TM, Rashid MHO, Hossain KA, Akhter R, Utsunomiya M, Kitab B, Hifumi T, Miyoshi N, Maetani F, Tsukiyama-Kohara K . Investigation of koala retrovirus in captive koalas with pneumonia and comparative analysis of subtype distribution. . Archives of virology168 ( 12 ) 298 2023年11月査読国際誌

Ushirozako G, Murayama N, Tsukiyama-Kohara K, Yamazaki H, Uno Y . Tree shrew cytochrome P450 2E1 is a functional enzyme that metabolises chlorzoxazone and p-nitrophenol. . Xenobiotica; the fate of foreign compounds in biological systems 1 - 11 2023年11月査読国際誌

Hossain KA, Akhter R, Rashid MHO, Akter L, Utsunomiya M, Kitab B, Mm N, Hishiki T, Kohara M, Morita K, Tsukiyama-Kohara K . Suppression of dengue virus replication by the French maritime pine extract Pycnogenol®. . Virus research 199244 2023年10月査読国際誌

Okamoto R, Ito N, Ide Y, Kitab B, Sakoda Y, Tsukiyama-Kohara K . Development of short hairpin RNA expression vectors targeting the internal ribosomal entry site of the classical swine fever virus genomic RNA. . BMC biotechnology23 ( 1 ) 37 2023年9月査読国際誌

Kenzaburo Yamaji, Sadahiro Iwabuchi, Yuko Tokunaga, Shinichi Hashimoto, Daisuke Yamane, Sakiko Toyama, Risa Kono, Bouchra Kitab, Kyoko Tsukiyama-Kohara*, Yosuke Osawa, Yukiko Hayashi, Tsunekazu Hishima, Chise Tateno, Kiminori Kimura, Takeshi Okanoue, Michinori Kohara . Molecular insights of a CBP/β-catenin-signaling inhibitor on nonalcoholic steatohepatitis-induced liver fibrosis and disorder . Biomedicine & Pharmacotherapy166 115379 2023年8月査読国際共著

Ushirozako G, Noda Y, Murayama N, Kawaguchi H, Tsukiyama-Kohara K, Yamazaki H, Uno Y . Newly Identified Tree Shrew Cytochrome P450 2A13 (CYP2A13) is Expressed in Liver and Lung and Encodes a Functional Drug-Metabolizing Enzyme Similar to Dog CYP2A13 and Pig CYP2A19. . Drug metabolism and disposition: the biological fate of chemicals 2023年5月査読国際誌

Uno Y, Jikuya S, Noda Y, Oguchi A, Murayama N, Kawaguchi H, Tsukiyama-Kohara K, Yamazaki H . Newly identified cytochrome P450 3A genes of tree shrews and pigs are expressed and encode functional enzymes. . Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 109579 2023年5月査読国際誌

Yoshida O, Akbar SMF, Imai Y, Sanada T, Tsukiyama-Kohara K, Miyazaki T, Kamishita T, Miyake T, Tokumoto Y, Hikita H, Tsuge M, Shimizu M, Al Mahtab M, Aguilar JC, Guillen G, Kohara M, Hiasa Y . Intranasal therapeutic vaccine containing HBsAg and HBcAg for patients with chronic hepatitis B; 18 months follow-up results of phase IIa clinical study. . Hepatology research : the official journal of the Japan Society of Hepatology 2023年3月査読国際誌

Uno Y, Noda Y, Murayama N, Tsukiyama-Kohara K, Yamazaki H . Novel cytochrome P450 1 (CYP1) genes in tree shrews are expressed and encode functional drug-metabolizing enzymes. . Comparative biochemistry and physiology. Toxicology & pharmacology : CBP265 109534 2023年3月査読国際誌

Kitab B, Tsukiyama-Kohara K . Regulatory Role of Ribonucleotide Reductase Subunit M2 in Hepatocyte Growth and Pathogenesis of Hepatitis C Virus. . International journal of molecular sciences24 ( 3 ) 2023年1月査読国際誌

Kayesh MEH, Khalil I, Kohara M, Tsukiyama-Kohara K . Increasing Dengue Burden and Severe Dengue Risk in Bangladesh: An Overview. . Tropical medicine and infectious disease8 ( 1 ) 2023年1月査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . TLR agonists as vaccine adjuvants in the prevention of viral infections: an overview. . Frontiers in microbiology14 1249718 2023年査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Epidemiology and Risk Factors for Acute Viral Hepatitis in Bangladesh: An Overview. . Microorganisms10 ( 11 ) 2022年11月査読国際誌

Uno Y, Ushirozako G, Uehara S, Murayama N, Fujiki Y, Kawaguchi H, Tsukiyama-Kohara K, Yamazaki H . Newly identified tree shrew cytochrome P450 2B6 (CYP2B6) and pig CYP2B6b are functional drug-metabolising enzymes. . Xenobiotica; the fate of foreign compounds in biological systems 1 - 10 2022年11月査読国際誌

Kayesh MEH, Hashem MA, Maetani F, Goto A, Nagata N, Kasori A, Imanishi T, Tsukiyama-Kohara K . Molecular Insights into Innate Immune Response in Captive Koala Peripheral Blood Mononuclear Cells Co-Infected with Multiple Koala Retrovirus Subtypes. . Pathogens (Basel, Switzerland)11 ( 8 ) 2022年8月査読国際誌

Characterization of host factors associated with the internal ribosomal entry sites of foot-and-mouth disease and classical swine fever viruses. . 12 ( 1 ) 6709 2022年4月査読国際共著

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . An Overview of Recent Insights into the Response of TLR to SARS-CoV-2 Infection and the Potential of TLR Agonists as SARS-CoV-2 Vaccine Adjuvants. . Viruses13 ( 11 ) 2021年11月査読国際誌

Kayesh MEH, Sanada T, Kohara M, Tsukiyama-Kohara K . Tree Shrew as an Emerging Small Animal Model for Human Viral Infection: A Recent Overview. . Viruses13 ( 8 ) 2021年8月査読国際誌

Hashem MA, Kayesh MEH, Maetani F, Eiei T, Mochizuki K, Ochiai S, Ito A, Ito N, Sakurai H, Asai T, Tsukiyama-Kohara K . Koala retrovirus (KoRV) subtypes and their impact on captive koala (Phascolarctos cinereus) health. . Archives of virology166 ( 7 ) 1893 - 1901 2021年7月査読国際誌

Makoto Saito1,*, Yasushi Itoh2,*, Fumihiko Yasui1,*, Tsubasa Munakata1, Daisuke Yamane1, Makoto Ozawa3, Risa Ito4, Takayuki Katoh4, Hirohito Ishigaki2, Misako Nakayama2, Shintaro Shichinohe2, Kenzaburo Yamaji1, Naoki Yamamoto1, Ai Ikejiri1, Tomoko Honda1, Takahiro Sanada1, Yoshihiro Sakoda5, Hiroshi Kida6, Le Thi Quynh Mai7, Yoshihiro Kawaoka8, Kazumasa Ogasawara2, Kyoko Tsukiyama-Kohara3,†, Hiroaki Suga4,†, and Michinori Kohara . Targeted macrocycles hamper hemagglutinin adsorption and fusion, and have antiviral effects in murine and macaque models of influenza . Nature Communications12 ( 1 ) 2654 2021年5月査読国際共著

Abounouh K. . Blocking neddylation elicits antiviral effect against hepatitis B virus replication . Molecular Biology Reports 2021年査読国際誌

Takagi A, Amako Y, Yamane D, Kitab B, Tokunaga Y, El-Gohary A, Kohara M, Tsukiyama-Kohara K . Longer Poly(U) Stretches in the 3'UTR Are Essential for Replication of the Hepatitis C Virus Genotype 4a Clone in <i>in vitro</i> and <i>in vivo</i>. . Frontiers in microbiology12 764816 2021年査読国際誌

Kayesh M.E.H. . Mammalian animal models for dengue virus infection: a recent overview . Archives of Virology167 ( 1 ) 31 - 44 2021年査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Toll-like Receptor Response to Human Immunodeficiency Virus Type 1 or Co-Infection with Hepatitis B or C Virus: An Overview. . International journal of molecular sciences24 ( 11 ) 2023年6月査読国際誌

Mohammad Enamul Hoque Kayesh, Md Abul Hashem, Takahiro Sanada, Bouchra Kitab, Md Haroon Or Rashid, Lipi Akter, Sayeh Ezzikouri, Shuko Murakami, Shintaro Ogawa, Yasuhito Tanaka, Michinori Kohara and Kyoko Tsukiyama-Kohara . Characterization of innate immune response to hepatitis B virus genotype F acute infection in tree shrew (Tupaia belangeri) model . Frontiers in Virology2 926831 2022年8月査読国際共著国際誌

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Frontiers Media SA

Hepatitis B virus (HBV) infection is a global public health problem. The clinical outcomes of HBV infections are influenced by host as well as viral factors, including viral genotypes and subgenotypes. The interplay between HBV and host innate immunity remains unclear because of the lack of a suitable small animal model. Tree shrews (Tupaia belangeri) have been utilized as a useful animal model for hepatitis viruses such as hepatitis B and C viruses. In this study, we characterized acute infections by HBV genotype F (HBV-F) wild type (Wt) and mutant type (Mt) viruses in adult tree shrews. Serum alanine aminotransferase levels were measured before and post- infection 7 and 14 dpi. Both HBV-F-Wt and Mt were detected in the HBV-F-infected tree shrew serum and liver tissue at 7 and 14 dpi. We examined the intrahepatic expression patterns of Toll-like receptors (TLRs) (TLR1–9 mRNAs), cGAS, several transcription factors such as STAT1, STAT2, IRF7, HNF4, PD-L1, and cytokines, including IFN-β, IFN-γ, IL-6, and TNF-α in HBV-F Wt/Mt-infected tree shrews. When compared with uninfected animal group, significant suppression of TLR8 in HBV-F-Wt infected animals and significant suppression of PD-L1 in both HBV-F-Wt and Mt infected animals were observed. Thus, tree shrew can be a useful animal model to characterize HBV-F pathogenesis.

DOI： 10.3389/fviro.2022.926831

Hashem M.A., Kayesh M.E.H., Maetani F., Goto A., Nagata N., Kasori A., Imanishi T., Tsukiyama-Kohara K. . Subtype distribution and expression of the koala retrovirus in the Japanese zoo koala population . Infection, Genetics and Evolution102 105297 - 105297 2022年8月査読国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Infection, Genetics and Evolution

We investigated the proviral copies and RNA expression in koala retrovirus (KoRV)-infected koalas. To ascertain any variation in viral load by institution, age, sex, or body condition score, we quantified KoRV proviral DNA and RNA loads in captive koalas (n = 37) reared in Japanese zoos. All koalas were positive for KoRV genes (pol, LTRs, and env of KoRV-A) in genomic DNA (gDNA), and 91.89% were positive for the pol gene in RNA. In contrast, the distribution rates of KoRV-B, KoRV-C, KoRV-D, and KoRV-F env genes in gDNA were 94.59%, 27.03%, 67.57%, and 54.05%, respectively. A wide inter-individual variation and/or a significant inter-institutional difference in proviral DNA (p < 0.0001) and RNA (p < 0.001) amounts (copies/103 koala β-actin copies) were observed in Awaji Farm England Hill Zoo koalas, which were obtained from southern koala populations, suggesting exogenous incorporation of KoRV in these koalas. Significant (p < 0.05) age differences were noted in KoRV RNA load (p < 0.05) and median total RNA load (p < 0.001), with loads higher in younger koalas (joeys and juveniles). Thus, the current study provides the distribution of KoRV subtypes in Japanese zoo koala populations and identifies several additional risk factors (sex, age, and body condition) associated with KoRV expression.

DOI： 10.1016/j.meegid.2022.105297

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Reverse vaccinology-based prediction of a multi-epitope SARS-CoV-2 vaccine and its tailoring to new coronavirus variants. . 2022年5月査読国際共著

Kayesh MEH, Kohara M, Tsukiyama-Kohara K. . Toll-like Receptor Response to Hepatitis C Virus Infection: A Recent Overview. . Int J Mol Sci.23 ( 10 ) 5475 2022年5月査読国際共著

Kayesh M.E.H., Kohara M., Tsukiyama-Kohara K. . Toll-like Receptor Response to Hepatitis C Virus Infection: A Recent Overview . International Journal of Molecular Sciences23 ( 10 ) 2022年5月査読国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：International Journal of Molecular Sciences

Hepatitis C virus (HCV) infection remains a major global health burden, causing chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Toll-like receptors (TLRs) are evolutionarily con-served pattern recognition receptors that detect pathogen-associated molecular patterns and acti-vate downstream signaling to induce proinflammatory cytokine and chemokine production. An in-creasing number of studies have suggested the importance of TLR responses in the outcome of HCV infection. However, the exact role of innate immune responses, including TLR response, in controlling chronic HCV infection remains to be established. A proper understanding of the TLR response in HCV infection is essential for devising new therapeutic approaches against HCV infection. In this review, we discuss the progress made in our understanding of the host innate immune response to HCV infection, with a particular focus on the TLR response. In addition, we discuss the mechanisms adopted by HCV to avoid immune surveillance mediated by TLRs.

DOI： 10.3390/ijms23105475

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Ezzemani W., Kettani A., Sappati S., Kondaka K., El Ossmani H., Tsukiyama-Kohara K., Altawalah H., Saile R., Kohara M., Benjelloun S., Ezzikouri S. . Reverse vaccinology-based prediction of a multi-epitope SARS-CoV-2 vaccine and its tailoring to new coronavirus variants . Journal of Biomolecular Structure and Dynamics 1 - 22 2022年5月査読国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biomolecular Structure and Dynamics

The genome feature of SARS-CoV-2 leads the virus to mutate and creates new variants of concern. Tackling viral mutations is also an important challenge for the development of a new vaccine. Accordingly, in the present study, we undertook to identify B- and T-cell epitopes with immunogenic potential for eliciting responses to SARS-CoV-2, using computational approaches and its tailoring to coronavirus variants. A total of 47 novel epitopes were identified as immunogenic triggering immune responses and no toxic after investigation with in silico tools. Furthermore, we found these peptide vaccine candidates showed a significant binding affinity for MHC I and MHC II alleles in molecular docking investigations. We consider them to be promising targets for developing peptide-based vaccines against SARS-CoV-2. Subsequently, we designed two efficient multi-epitopes vaccines against the SARS-CoV-2, the first one based on potent MHC class I and class II T-cell epitopes of S (FPNITNLCPF–NYNYLYRLFR–MFVFLVLLPLVSSQC), M (MWLSYFIASF–GLMWLSYFIASFRLF), E (LTALRLCAY–LLFLAFVVFLLVTLA), and N (SPRWYFYYL–AQFAPSASAFFGMSR). The second candidate is the result of the tailoring of the first designed vaccine according to three classes of SARS-CoV-2 variants. Molecular docking showed that the protein-protein binding interactions between the vaccines construct and TLR2–TLR4 immune receptors are stable complexes. These findings confirmed that the final multi-epitope vaccine could be easily adapted to new viral variants. Our study offers a shortlist of promising epitopes that can accelerate the development of an effective and safe vaccine against the virus and its adaptation to new variants. Communicated by Ramaswamy H. Sarma.

DOI： 10.1080/07391102.2022.2075468

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Hashem MA, Kayesh MEH, Maetani F, Goto A, Nagata N, Kasori A, Imanishi T, Tsukiyama-Kohara K . Subtype distribution and expression of the koala retrovirus in the Japanese zoo koala population. . Infect Genet Evol. 105297 2022年5月査読国際共著

Ide Y., Kitab B., Ito N., Okamoto R., Tamura Y., Matsui T., Sakoda Y., Tsukiyama-Kohara K. . Characterization of host factors associated with the internal ribosomal entry sites of foot-and-mouth disease and classical swine fever viruses . Scientific Reports12 ( 1 ) 6709 - 6709 2022年4月査読国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Scientific Reports

Foot-and-mouth disease virus (FMDV) and classical swine fever virus (CSFV) possess positive-sense single-stranded RNA genomes and an internal ribosomal entry site (IRES) element within their 5′-untranslated regions. To investigate the common host factors associated with these IRESs, we established cell lines expressing a bicistronic luciferase reporter plasmid containing an FMDV-IRES or CSFV-IRES element between the Renilla and firefly luciferase genes. First, we treated FMDV-IRES cells with the French maritime pine extract, Pycnogenol (PYC), and examined its suppressive effect on FMDV-IRES activity, as PYC has been reported to have antiviral properties. Next, we performed microarray analysis to identify the host factors that modified their expression upon treatment with PYC, and confirmed their function using specific siRNAs. We found that polycystic kidney disease 1-like 3 (PKD1L3) and ubiquitin-specific peptidase 31 (USP31) were associated with FMDV-IRES activity. Moreover, silencing of these factors significantly suppressed CSFV-IRES activity. Thus, PKD1L3 and USP31 are host factors associated with the functions of FMDV- and CSFV-IRES elements.

DOI： 10.1038/s41598-022-10437-z

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Kayesh M.E.H., Hashem M.A., Kohara M., Tsukiyama-Kohara K. . In vivo Delivery Tools for Clustered Regularly Interspaced Short Palindromic Repeat/Associated Protein 9-Mediated Inhibition of Hepatitis B Virus Infection: An Update . Frontiers in Microbiology13 953218 - 953218 2022年査読国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Frontiers in Microbiology

Chronic hepatitis B virus (HBV) infection remains a major global health problem despite the availability of an effective prophylactic HBV vaccine. Current antiviral therapies are unable to fully cure chronic hepatitis B (CHB) because of the persistent nature of covalently closed circular DNA (cccDNA), a replicative template for HBV, which necessitates the development of alternative therapeutic approaches. The CRISPR/Cas system, a newly emerging genome editing tool, holds great promise for genome editing and gene therapy. Several in vitro and/or in vivo studies have demonstrated the effectiveness of HBV-specific clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (CRISPR/Cas9) systems in cleaving HBV DNA and cccDNA. Although recent advances in CRISPR/Cas technology enhance its prospects for clinical application against HBV infection, in vivo delivery of the CRISPR/Cas9 system at targets sites remains a major challenge that needs to be resolved before its clinical application in gene therapy for CHB. In the present review, we discuss CRISPR/Cas9 delivery tools for targeting HBV infection, with a focus on the development of adeno-associated virus vectors and lipid nanoparticle (LNP)-based CRISPR/Cas ribonucleoprotein (RNP) delivery to treat CHB. In addition, we discuss the importance of delivery tools in the enhancement of the antiviral efficacy of CRISPR/Cas9 against HBV infection.

DOI： 10.3389/fmicb.2022.953218

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Kayesh M.E.H. . Toll-like receptor response to hepatitis Bb virus infection and potential of TLR agonists as immunomodulators for treating chronic hepatitis b: An overview . International Journal of Molecular Sciences22 ( 19 ) 2021年9月査読国際誌

Abbadi I. . Non-primate hepacivirus transmission and prevalence: Novel findings of virus circulation in horses and dogs in Morocco . Infection, Genetics and Evolution93 104975 - 104975 2021年9月査読国際誌

ケアシュ・モハメド, 尼子豊, ハシェム・モハメド, 山本直樹, 杉山真也, 溝上雅史, 小原道法, 小原恭子 . B型肝炎ウイルスcccDNAを標的としたCRISPR/Cas9システムの生体内送達法の開発 . 日本癌学会総会記事80回 [J3 - 5] 2021年9月査読国際誌

Honda T, Gomi S, Yamane D, Yasui F, Yamamoto T, Munakata T, Itoh Y, Ogasawara K, Sanada T, Yamaji K, Yasutomi Y, Tsukiyama-Kohara K, Kohara M. . Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA . mSphere6 ( 3 ) e1342-20 2021年5月査読

Honda T. . Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies . mSphere6 ( 3 ) 1 - 14 2021年5月査読国際誌

Saito M. . Macrocyclic peptides exhibit antiviral effects against influenza virus HA and prevent pneumonia in animal models . Nature Communications12 ( 1 ) 2654 - 2654 2021年5月査読国際誌

Toll-Like Receptor and Cytokine Responses to Infection with Endogenous and Exogenous Koala Retrovirus, and Vaccination as a Control Strategy . Curr Issues Mol Biol. 43 ( 1 ) 52 - 64 2021年4月査読国際共著

Md Abul Hashem1, 2, 3, Mohammad Enamul Hoque Kayesh1, 2, 4, Fumie Maetani5, Taiki Eiei5, Kyoya Mochizuki5, Shinsaku Ochiai5, Ayaka Ito5, Nanao Ito5, Hiroko Sakurai5, Takayuki Asai5 and Kyoko Tsukiyama-Kohara . Koala retrovirus (KoRV) subtypes and their impact on captive koala (Phascolarctos cinereus) health . Archives of Virologyaccepted 2021年4月査読

Mohammad Enamul Hoque Kayesh 1,2 , Md Abul Hashem 1,3,4 and Kyoko Tsukiyama-Kohara . Toll-Like Receptor Expression Profiles in Koala (Phascolarctos cinereus) Peripheral Blood Mononuclear Cells Infected with Multiple KoRV Subtypes . 11 983 2021年4月査読

Kayesh M.E.H., Hashem M.A., Tsukiyama-Kohara K. . Toll-like receptor expression profiles in koala (Phascolarctos cinereus) peripheral blood mononuclear cells infected with multiple korv subtypes . Animals11 ( 4 ) 2021年4月査読国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Animals

Toll-like receptors (TLRs), evolutionarily conserved pattern recognition receptors, play an important role in innate immunity by recognizing microbial pathogen-associated molecular patterns. Koala retrovirus (KoRV), a major koala pathogen, exists in both endogenous (KoRV-A) and exogenous forms (KoRV-B to J). However, the expression profile of TLRs in koalas infected with KoRV-A and other subtypes is yet to characterize. Here, we investigated TLR expression profiles in koalas with a range of subtype infection profiles (KoRV-A only vs. KoRV-A with KoRV-B and/or-C). To this end, we cloned partial sequences for TLRs (TLR2–10 and TLR13), developed real-time PCR assays, and determined TLRs mRNA expression patterns in koala PBMCs and/or tissues. All the reported TLRs for koala were expressed in PBMCs, and variations in TLR expression were observed in koalas infected with exogenous subtypes (KoRV-B and KoRV-C) compared to the endogenous subtype (KoRV-A) only, which indicates the implications of TLRs in KoRV infection. TLRs were also found to be differentially expressed in koala tissues. This is the first report of TLR expression profiles in koala, which provides insights into koala’s immune response to KoRV infection that could be utilized for the future exploitation of TLR modulators in the maintenance of koala health.

DOI： 10.3390/ani11040983

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Nagai M, Okabayashi T, Akagami M, Matsuu A, Fujimoto Y, Hashem MA, Mekata H, Nakao R, Matsuno K, Katayama Y, Oba M, Omatsu T, Asai T, Nakagawa K, Ito H, Madarame H, Kawai K, Ito T, Nonaka N, Tsukiyama-Kohara K, Inoshima Y, Mizutani T, Misawa N. . Metagenomic identification, sequencing, and genome analysis of porcine hepe-astroviruses (bastroviruses) in porcine feces in Japan . Infect Genet Evol.88 104664 2021年3月査読

Nagai M., Okabayashi T., Akagami M., Matsuu A., Fujimoto Y., Hashem M.A., Mekata H., Nakao R., Matsuno K., Katayama Y., Oba M., Omatsu T., Asai T., Nakagawa K., Ito H., Madarame H., Kawai K., Ito T., Nonaka N., Tsukiyama-Kohara K., Inoshima Y., Mizutani T., Misawa N. . Metagenomic identification, sequencing, and genome analysis of porcine hepe-astroviruses (bastroviruses) in porcine feces in Japan . Infection, Genetics and Evolution88 104664 - 104664 2021年3月査読国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Infection, Genetics and Evolution

Recently, hepe-astrovirus-like RNA viruses named bastroviruses (BastVs), have been found in human, pig, bat, and rat fecal samples. In this study, we determined nearly complete genome sequences of four BastVs in the feces of healthy pigs. Genetic characterization revealed that these porcine BastVs (PBastVs) and BastVs from other animals including humans, had the same genome organization, that is, they contained three predicted conserved domains of viral methyltransferase, RNA helicase, and RdRp in the nonstructural ORF1 and the astrovirus capsid domain in the structural ORF2. Phylogenetic analyses using RNA-dependent RNA polymerase and the capsid region revealed that PBastVs branched with bat and rat BastVs; however, the groups formed by each host were distantly related to human BastVs. Pairwise amino acid sequence comparison demonstrated that PBastVs shared 95.2–98.6% and 76.1–95.5% sequence identity among each other in the ORF1 and ORF2 regions, respectively; the sequence identities between PBastVs and BastVs from other animals were 21.4–42.5% and 9.1–20.6% in the ORF1 and ORF2 regions, respectively. This suggested that BastVs were derived from a common ancestor but evolved independently in each host population during a prolonged period. Putative recombination events were identified in the PBastV genome, suggesting that PBastVs gain sequence diversity and flexibility through recombination events. In an analysis of previously obtained metagenomic data, PBastV sequence reads were detected in 7.3% (23/315) of fecal samples from pigs indicating that PBastVs are distributed among pig populations in Japan.

DOI： 10.1016/j.meegid.2020.104664

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Suzuki Y, Onuma H, Sato R, Sato Y, Hashiba A, Maeki M, Tokeshi M, Kayesh MEH, Kohara M, Tsukiyama-Kohara K, Harashima H. . Lipid nanoparticles loaded with ribonucleoprotein-oligonucleotide complexes synthesized using a microfluidic device exhibit robust genome editing and hepatitis B virus inhibition. . 330 67 - 71 2021年2月査読

Suzuki Y. . Lipid nanoparticles loaded with ribonucleoprotein–oligonucleotide complexes synthesized using a microfluidic device exhibit robust genome editing and hepatitis B virus inhibition . Journal of Controlled Release330 61 - 71 2021年2月査読国際誌

Kayesh M.E.H. . Recent Insights Into the Molecular Mechanism of Toll-Like Receptor Response to Dengue Virus Infection . Frontiers in Microbiology12 744233 - 744233 2021年査読国際誌

CD4, CD8b, and Cytokines Expression Profiles in Peripheral Blood Mononuclear Cells Infected with Different Subtypes of KoRV from Koalas (Phascolarctos cinereus) in a Japanese Zoo. . Viruses12 ( 12 ) 1415 2020年12月

Kayesh M.E.H. . Cd4, cd8b, and cytokines expression profiles in peripheral blood mononuclear cells infected with different subtypes of korv from koalas (Phascolarctos cinereus) in a japanese zoo . Viruses12 ( 12 ) 2020年12月査読国際誌

Ezzikouri S, Nourlil J, Benjelloun S, Kohara M, Tsukiyama-Kohara K. . Coronavirus disease 2019-Historical context, virology, pathogenesis, immunotherapy, and vaccine development. . Hum Vaccin Immunother. 16 ( 12 ) 2992 - 3000 2020年12月招待査読

Kayesh MEH, Amako Y, Hashem MA, Murakami S, Ogawa S, Yamamoto N, Hifumi T, Miyoshi N, Sugiyama M, Tanaka Y, Mizokami M, Kohara M, Tsukiyama-Kohara K. . Development of an in vivo delivery system for CRISPR/Cas9-mediated targeting of hepatitis B virus cccDNA . Virus Research290 198191 2020年12月査読

Ezzikouri S. . Coronavirus disease 2019—Historical context, virology, pathogenesis, immunotherapy, and vaccine development . Human Vaccines and Immunotherapeutics16 ( 12 ) 2992 - 3000 2020年12月査読国際誌

Elmessaoudi-Idrissi M, Tsukiyama-Kohara K, Nourlil J, Kettani A, Windisch MP, Kohara M, Malik YS, Dhama K, Benjelloun S, Ezzikouri S. . Structure-guided discovery approach identifies potential lead compounds targeting M pro of SARS-CoV-2 . Virusdisease 2020年11月査読

Ezzikouri S. . Nanobodies: an unexplored opportunity to combat COVID-19 . Journal of Biomolecular Structure and Dynamics 1 - 3 2020年11月査読国際誌

Kayesh MEH, Hashem MA, Tsukiyama-Kohara K. . Koala retrovirus epidemiology, transmission mode, pathogenesis, and host immune response in koalas (Phascolarctos cinereus): a review. . Arch Virol. 165 ( 11 ) 2409 - 2417 2020年11月招待査読

Ezzikouri S, Nourlil J, Tsukiyama-Kohara K, Kohara M, El Ossmani H, Windisch MP, Benjelloun S. . Nanobodies: an unexplored opportunity to combat COVID-19 . J Biomol Struct Dyn.10 1 - 3 2020年11月査読

Kayesh M.E.H. . Koala retrovirus epidemiology, transmission mode, pathogenesis, and host immune response in koalas (Phascolarctos cinereus): a review . Archives of Virology165 ( 11 ) 2409 - 2417 2020年11月査読国際誌

Hashem MA, Maetani F, Kayesh MEH, Eiei T, Mochizuki K, Ito A, Sakurai H, Asai T, Tsukiyama-Kohara K. . Transmission of koala retrovirus from parent koalas to a joey in a Japanese zoo. . Journal of Virolgy94 ( 11 ) e0019-20 2020年5月査読

Chi Hai-Ying, Yuuki Tanaka, Tatsuro Hifumi, Ko-ichiro Shoji, Mohammad Enamul Hoque Kayesh, Md Abul Hashem, Bouchra Kitab, Takahiro Sanada, Tomoko Fujiyuki, Misako Yoneda, Hitoshi Hatai, Akira Yabuki, Noriaki Miyoshi, Chieko Kai, Michinori Kohara, Kyoko Tsukiyama-Kohara . Pathological and genetic aspects of spontaneous mammary gland tumor in Tupaia belangeri (Tree shrew) . PLoS One15 ( 5 ) e0233232 2020年5月査読

Sayeh Ezzikouri, Mohammad Enamul Hoque Kayesh, Soumaya Benjelloun, Michinori Kohara and Kyoko Tsukiyama-Kohara . Targeting host innate and adaptive immunity to achieve the functional cure of chronic hepatitis B . Vaccines8 ( 2 ) E216 2020年5月査読

Mohammad Enamul Hoque Kayesh 1,2 , Md Abul Hashem 1, Bouchra Kitab 1 and Kyoko Tsukiyama-Kohara . Pathogenesis and Immune Response Caused by Vector-Borne and Other Viral Infections in a Tupaia Model . microorganism 2019年12月招待査読

Sanada T, Yamamoto N, Kayesh MEH, Tsukiyama-Kohara K, Hasegawa H, Miyazaki T, Takano JI, Shiogama Y, Yasutomi Y, Goh Y, Yoshida O, Hiasa Y, Kohara M. . Intranasal vaccination with HBs and HBc protein combined with carboxyl vinyl polymer induces strong neutralizing antibody, anti-HBs IgA, and IFNG response. . Biochem Biophys Res Commun.520 ( 1 ) 86 - 92 2019年11月査読

Sanada T. . Intranasal vaccination with HBs and HBc protein combined with carboxyl vinyl polymer induces strong neutralizing antibody, anti-HBs IgA, and IFNG response . Biochemical and Biophysical Research Communications520 ( 1 ) 86 - 92 2019年11月査読国際誌

Hashem MA, Kayesh MEH, Yamato O, Maetani F, Eiei T, Mochizuki K, Sakurai H, Ito A, Kannno H, Kasahara T, Amano Y, Tsukiyama-Kohara K. . Coinfection with koala retrovirus subtypes A and B and its impact on captive koalas in Japanese zoos. . Arch Virol. 164 ( 11 ) 2735 - 2745 2019年11月査読

Hashem M. . Coinfection with koala retrovirus subtypes A and B and its impact on captive koalas in Japanese zoos . Archives of Virology164 ( 11 ) 2735 - 2745 2019年11月査読国際誌

Sanada T, Tsukiyama-Kohara K, Shin-I T, Yamamoto N, Kayesh MEH, Yamane D, Takano JI, Shiogama Y, Yasutomi Y, Ikeo K, Gojobori T, Mizokami M, Kohara M. . Construction of complete Tupaia belangeri transcriptome database by whole-genome and comprehensive RNA sequencing. . Sci Rep. 9 ( 1 ) 12372 2019年8月査読

Sanada T. . Construction of complete Tupaia belangeri transcriptome database by whole-genome and comprehensive RNA sequencing . Scientific Reports9 ( 1 ) 12372 - 12372 2019年8月査読国際誌

Matsui T, Handa Y, Kanda T, Tsukiyama-Kohara K. . Silencing of the foot-and-mouth disease virus internal ribosomal entry site by targeting relatively conserved region among serotypes. . Virus Genes. 2019年7月査読

Kitab B, Satoh M, Ohmori Y, Munakata T, Sudoh M, Kohara M, Tsukiyama-Kohara K . Ribonucleotide reductase M2 promotes RNA replication of hepatitis C virus by protecting NS5B protein from hPLIC1-dependent proteasomal degradation. . J. Biol. Chem.294 ( 15 ) 5759 - 5773 2019年4月査読

Sanada T, Yasui F, Honda T, Kayesh MEH, Takano JI, Shiogama Y, Yasutomi Y, Tsukiyama-Kohara K, Kohara M. . Avian H5N1 influenza virus infection causes severe pneumonia in the Northern tree shrew (Tupaia belangeri). . Virology529 101 - 110 2019年3月査読

Kayesh MEH, Yamato O, Rahman MM, Hashem MA, Maetani F, Eiei T, Mochizuki K, Sakurai H, Tsukiyama-Kohara K. . Molecular dynamics of koala retrovirus infection in captive koalas in Japan. . Arch. Virol.164 ( 3 ) 757 - 765 2019年3月査読

Sanada T. . Avian H5N1 influenza virus infection causes severe pneumonia in the Northern tree shrew (Tupaia belangeri) . Virology529 101 - 110 2019年3月査読国際誌

Kitab, B., Kohara, M., Tsukiyama-Kohara, K. . Experimental in vitro and in vivo systems for studying the innate immune response during dengue virus infections. . Arch Virol.163 ( 7 ) 1717 - 1726 2018年7月査読

Kyoko Tsukiyama-Kohara, Michinori Kohara . Hepatitis C Virus: Viral Quasispecies and Genotype . International Journal of Molecular Sciences19 ( 23 ) 1 - 8 2017年12月招待査読

Tsukiyama-Kohara K. . Hepatitis C virus: Viral quasispecies and genotypes . International Journal of Molecular Sciences19 ( 1 ) 2017年12月査読国際誌

Nze-Nkogue C, Horie M, Fujita S, Inoue E, Akomo-Okoue EF, Ozawa M, Ngomanda A, Yamagiwa J, Tsukiyama-Kohara K. . Identification and molecular characterization of novel primate bocaparvoviruses from wild western lowland gorillas of Moukalaba-Doudou National Park, Gabon. . Infect Genet Evol.53 30 - 37 2017年9月査読

Chimene NZE NKOGUE . Epidemiological study of viruses infecting western lowland gorillas in Moukalaba-Doudou National Park (Gabon) . 2017年9月査読

Kayesh MEH, Ezzikouri S, Chi H, Sanada T, Hayashi, Y., Rebbani, K., Kitab, B., Matuu, A., Miyoshi, N., Hishima, T., Kohara, M., Tsukiyama-Kohara, K. . Oxidative Stress and Immune Responses During Hepatitis C Virus Infection in Tupaia belangeri. . Scientific Reports7 ( 1 ) 9848 - 9861 2017年8月査読

Kayesh ME*, Kitab B*, Sanada T, Hayasaka D, Morita K, Kohara M, Tsukiyama-Kohara K . Susceptibility of tupaia cells to dengue virus infection and their initial immune response. . Infection Genetics and Evolution51 203 - 210 2017年7月査読

KAYESH MEH, EZZIKOURI S, CHI H, SANADA T, YAMAMOTO N, KITAB B, HARAGUCHI T, MATUYAMA R, NKOGUE CN, HATAI H, MIYOSHI N, MURAKAMI S, TANAKA Y, TAKANO J, SHIOGAMA Y, YASUTOMI Y, KOHARA M, TSUKIYAMA-KOHARA K. . Interferon-β response is impaired by hepatitis B virus infection in Tupaia belangeri. . VIRUS RESEARCH237 47 - 57 2017年6月査読

Tokunaga Y, Osawa Y, Ohtsuki T, Hayashi Y, Yamaji K, Yamane D, Hara M, Munekata K, Tsukiyama-Kohara K, Hishima T, Kojima S, Kimura K, Kohara M. . Selective inhibitor of Wnt/β-catenin/CBP signaling ameliorates hepatitis C virus-induced liver fibrosis in mouse model. . Sci Rep.7 ( 1 ) 325 2017年3月査読

Chime`ne Nze Nkogue, Masayuki Horie, Shiho Fujita, Michiko Ogino, Yuki Kobayashi, Keijiro Mizukami, Tatsunori Masatani, Sayeh Ezzikouri, Aya Matsuu, Tetsuya Mizutani, Makoto Ozawa, Osamu Yamato, Alfred Ngomanda, Juichi Yamagiwa, Kyoko Tsukiyama-Kohara . Molecular epidemiological study of adenovirus infecting western lowland gorillas and humans in and around Moukalaba-Doudou National Park (Gabon) . Virus Genes52 ( 5 ) 671 - 678 2016年10月査読

Sayeh Ezzikouri, Fatima Zahra Jadid, Salsabil Hamdi, Kyoko Tsukiyama-Kohara, Soumaya Benjelloun . Supplementation of Pycnogenol to conventional treatment may improve type 2 diabetes-associated with Hepatitis C Therapy . Journal of Clinical and Translational Hepatology4 ( 3 ) 228 - 233 2016年9月査読

Takahisa Kouwaki, Yoshimi Fukushima, Takuji Daito, Naoki Yamamoto, Edin J Mifsud, Chean Ring Leong, Kyoko Tsukiyama-Kohara, Michinori Kohara, Misako Matsumoto, Tsukasa Seya, Hiroyuki Oshiumi . Exosome RNA Released by Hepatocytes Regulates Innate Immune Responses to Hepatitis B Virus Infection . Frontiers in Immunology7 335 2016年8月査読

Hai-Ying C, Nagano K, Ezzikouri S, Yamaguchi C, Kayesh ME, Rebbani K, Kitab B, Nakano H, Kouji H, Kohara M, Tsukiyama-Kohara K. . Establishment of an intermittent cold stress model using Tupaia belangeri and evaluation of compound C737 targeting neuron-restrictive silencer factor. . Exp. Anim.65 ( 3 ) 285 - 292 2016年7月査読

Rebban, K & iTsukiyama-Kohara, K. . HCV-Induced Oxidative Stress: Battlefield-Winning Strategy . Oxidative Medicine and Cellular Longevity2016 ( 2016 ) 7425628 2016年5月招待査読

Kanda T, Ozawa M, Tsukiyama-Kohara K . IRES-mediated translation of foot-andmouth disease virus (FMDV) in cultured cells derived from FMDV-susceptible and -insusceptible animals . BMC Veterinary Research12 66 2016年3月査読

Sanada, T., Tsukiyama-Kohara, K., Yamamoto, N., Ezzikouri, S., Benjelloun, S., Murakami, S., Tanaka, Y., Tateno, C., Kohara, M. . Property of hepatitis B virus replication in tupaia belangeri hepatocytes . BBRC8 ( 469 ) 229 - 235 2016年1月査読

Khadija Rhebbani, Kyoko Tsukiyama-Kohara . DHCR24:A Novel HCC Biomaker at a Glance . Clinical Microbiology4 ( 6 ) 1000228 2015年12月招待査読

Masatani T, Ozawa M, Yamada K, Ito N, Horie M, Matsuu A, Okuya K, Tsukiyama-Kohara K, Sugiyama M, Nishizono A. . Contribution of the interaction between rabies virus P protein and IKKε to the inhibition of type I interferon induction signaling. . J Gen. Virol. 2015年12月査読

Ohtsuki T, Kimura K, Tokunaga Y, Tsukiyama-Kohara K, Tateno C, Hayashi Y, Hishima T, Kohara M. . M2 macrophages play critical roles in progression of inflammatory liver disease in hepatitis C virus transgenic mice. . J Virol.14 ( 90 ) 307 - 309 2015年10月査読

Okuya K, Kawabata T, Nagano K, Tsukiyama-Kohara K, Kusumoto I, Takase K, Ozawa M. . Isolation and characterization of influenza A viruses from environmental water at an overwintering site of migratory birds in Japan. . Arch Virol. 2015年9月査読

Ozawa M, Kawabata T, Okuya K, Nagano K, Kanda T, Kanazawa N, Tsukiyama-Kohara K, Taneno A, Deguchi E. . Full genome sequences of torque teno sus virus strains that coinfected a pig with postweaning multisystemic wasting syndrome in Japan: implications for genetic diversity. . Arch Virol. 2015年9月査読

Miura R, Kooriyama T, Yoneda M, Takenaka A, Doki M, Goto Y, Sanjoba C, Endo Y, Fujiyuki T, Sugai A, Tsukiyama-Kohara K, Matsumoto Y, Sato H, Kai C. . Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs. . PLoS Negl Trop Dis.10 ( 9 ) e0003914 2015年7月査読

Matsuu A, Hobo S, Ando K, Sanekata T, Sato F, Endo Y, Amaya T, Osaki T, Horie M, Masatani T, Ozawa M, Tsukiyama-Kohara K . Genetic and serological surveillance for non-primate hepacivirus inhorses in Japan . Veterinary Microbiologyin press 2015年6月査読

Sayeh Ezzikouri, Kiminori Kimura, Hajime Sunagozaka, Shuichi Kaneko, Kazuaki Inoue, Tomohiro Nishimura, Tsunekazu Hishima, Michinori Kohara, and Kyoko Tsukiyama-Kohara . Serum DHCR24 auto-antibody as a new biomarker for progression of hepatitis C . EBioMedicine2 604 - 612 2015年4月査読

Makoto Saito, Takashi Takano, Tomohiro Nishimura, Michinori Kohara, Kyoko Tsukiyama-Kohara . 3β-Hydroxysterol Δ24-reductase on the Surface of Hepatitis C Virus-related Hepatocellular Carcinoma Cells can be a Target for Molecular Targeting Therapy . PLoS One e0124197 2015年4月査読

Ozawa M, Matsuu A, Yonezawa K, Igarashi M, Okuya K, Kawabata T, Ito K, Tsukiyama-Kohara K, Taneno A, Deguchi E. . Efficient isolation of Swine influenza viruses by age-targeted specimen collection. . J Clin Microbiol. 201553 ( 4 ) 1331 - 1338 2015年4月査読

新井　正明 . Establishment of evaluation systems of antiviral agents for hepatitis C virus and analysis of cyclophilin inhibitors as anti-HCV drugs . 2015年3月査読

Ezzikouri S, Nishimura T, Kohara M, Benjelloun S, Kino Y, Inoue K, Matsumori A, Tsukiyama-Kohara K . Inhibitory Effects of PycnogenolR on Hepatitis C Virus Replication . Antiviral Research113 93 - 102 2015年1月査読

Takenaka A, Yoneda M, Seki T, Uema M, Kooriyama T, Nishi T, Fujita K, Miura R, Tsukiyama-Kohara K, Sato H, Kai C. . Characterization of two recent Japanese field isolates of canine distemper virus and examination of the avirulent strain utility as an attenuated vaccine. . Vet Microbiol. 174 ( 42067 ) 372 - 381 2014年12月査読

Kyoko Tsukiyama-Kohara and Michinori Kohara . Tupaia belangeri as an experimental animal model for viral infection . Experimental Animal63 ( 4 ) 367 - 374 2014年10月査読

Mohammad Zare-Bidaki, Kyoko Tsukiyama-Kohara, Mohammad Kazemi Arababadi . Toll like receptor 4 and hepatitis B infection; molecular mechanisms and pathogenesis . Viral Immunology27 ( 7 ) 321 - 326 2014年9月査読

Ezzikouri S, Ozawa M, Kohara M, Elmdaghri N, Benjelloun S, Tsukiyama-Kohara K. . Recent insights into hepatitis B virus-host interactions. . J Med Virol.86 ( 6 ) 925 - 932 2014年6月査読

Arai M, Tsukiyama-Kohara K, Takagi A, Tobita Y, Inoue K, Kohara M. . Resistance to cyclosporin A derives from mutations in hepatitis C virus nonstructural proteins. . Biochem Biophys Res Commun.448 ( 1 ) 56 - 62 2014年5月査読

Kasama Y, Mizukami T, Kusunoki H, Peveling-Oberhag J, Nishito Y, Ozawa M, Kohara M, Mizuochi T, Tsukiyama-Kohara K. . B-Cell-Intrinsic Hepatitis C Virus Expression Leads to B-Cell-Lymphomagenesis and Induction of NF-κB Signalling. . PLos One9 ( 3 ) e91373 2014年3月査読

勝目朝夫 . Preclinical evaluation of an anti-interleukin 6 receptor antibody for Castleman's disease and a serene palmitoyltransferase inhibitor as an antiviral agent for hepatitis C virus . 2014年3月査読

Katsume A, Tokunaga Y, Hirata Y, Munakata T, Saito M, Hayashi H, Okamoto Y, Kusanagi I, Fujiwara S, Klumpp K, Tsukiyama-Kohara K, El-Gohary A, Sudoh M, Kohara M . A serine palmitoyltransferase inhibitor blocks hepatitis C virus replication in human hepatocytes . Gastroenterology145 865 - 873 2013年10月査読

Kyoko Tsukiyama-Kohara, Asao Katsume,Kazuhiro Kimura, Masayuki Saito, and MichinoriKohara . 4E-BP1 regulates the differentiation of white adipose tissue . Genes to Cells18 ( 7 ) 602 - 607 2013年7月査読

Nakagawa S, Hirata Y, Kameyama T, Tokunaga Y,Nishito Y, Yano J, Ochiya T, Tateno C, MizokamiM, Tsukiyama-Kohara K, Inoue K, Yoshiba M,Takaoka A, Kohara M. . Targeted induction of interferon-λ in humanized chimeric mouse liver abrogates hepatotropic virusinfection. . PLoS Onee59611 2013年3月査読

Salem NE, Saito M, Kasama Y, Ozawa M,Kawabata T, Harada S, Suda H, Asonuma K, El-Gohary A, Tsukiyama-Kohara, K. . Genomic polymorphisms in 3β-hydroxysterol Δ24-reductase promoter sequences. . Microbiol. Immunol.57 179 - 184 2013年3月査読

S. Sekiguchi, K. Kimura, T. Chiyo, T. Ohtsuki, Y. Tobita, Y. Tokunaga, F. Yasui, K. Tsukiyama-Kohara, T. Wakita, T. Tanaka, M. Miyasaka, K. Mizuno, Y. Hayashi, T. Hishima, K. Matsushima and M. Kohara . Immunization with a recombinant vaccinia virus that encodes nonstructural proteins of the hepatitis C virus suppresses viral protein levels in mouse liver. . Pros Onee51656 2012年11月査読

K. Inoue, K. Tsukiyama-Kohara, C. Matsuda, M. Yoneyama, T. Fujita, S. Kuge, M. Yoshiba, and *M. Kohara. . Impairment of interferon regulatory factor-3 activation by hepatitis C virus core protein basic amino acid region 1. . Biochem. Biophys. Res. Com. 428 494 - 499 2012年10月査読

Saito M, Kohara M, *Tsukiyama-Kohara K. . Hepatitis C virus promotes expression of the 3β-hydroxysterol δ24-reductase through Sp1. . Journal of Medical Virology84 ( 5 ) 733 - 746 2012年5月査読

Y. Kasama, M. Saito, T. Takano, T. Nishimura, M. Satoh, Z. Wang, S.N. E. S. Ali, S. Harada, M. Kohara, and *K. Tsukiyama-Kohara. . Translocase of outer mitochondrial membrane 70 induces interferon response and is impaired by hepatitis C virus NS3. . Virus Research 163 ( 1 ) 405 - 409 2012年1月査読

M. Satoh, M. Saito, T.Takano, Y. Kasama, T. Nishimura, Y. Nishito, Y. Hirata, M. Arai, M. Sudoh, C. Kai, M. Kohara and *K. Tsukiyama-Kohara . Monoclonal antibody 2-152a suppresses hepatitis C virus infection thorough betaine/GABA transporter-1. . Journal of Infectious Diseses204 1172 - 1180 2011年10月査読

T. Takano, *K. Tsukiyama-Kohara, M. Hayashi, Y. Hirata, M. Satoh, C. Tateno, Y. Hayashi, T. Hishima, N. Funata, M. Sudo, and M. Kohara. . Augmentation of DHCR24 expression by hepatitis C virus infection facilitates viral replication in hepatocytes. . Journal of Hepatology55 512 - 521 2011年8月査読

Y. Kasama, M. Satoh, M. Saito, S. Okada, C. Kai and *K. Tsukiyama-Kohara. . Evaluation of a recombinant measles virus as the expression vector of hepatitis C virs envelope proteins. . World Journal of Vaccine1 98 - 103 2011年8月査読

K. Tsukiyama-Kohara, S. Sekiguchi, Y. Kasama, N. E. S. Ali, K. Machida and M. Kohara . Hepatitis C virus-related lymphomagenesis in a mouse model. . ISRN Hematology2011 2011年6月査読

T. Takano, M. Kohara, M. Kasama, T. Nishimura, M. Saito, C. Kai and *K. Tsukiyama-Kohara . Translocase of outer mitochondrial membrane 70 expression is induced by hepatitis C virus and is related to the apoptotic response. . Journal of Medical Virology83 801 - 809 2011年3月査読

Selective translation of the measles virus nucleocapsid mRNA by la protein. Y. Inoue, H. Sato, K. Fujita, K. Tsukiyama-Kohara, M.Yoneda, and *C. Kai. . Selective translation of the measles virus nucleocapsid mRNA by la protein. . Front. Microbiol. 2 173 2011年1月査読

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