研究者詳細 - 小原　恭子

論文 - 小原　恭子

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Uno Y, Kamizono M, Ushirozako G, Tsukiyama-Kohara K, Murayama N, Yamazaki H . Novel cytochrome P450 2J isoforms in tree shrews are functional drug-metabolising enzymes involved in ebastine and astemizole oxidation. . Xenobiotica; the fate of foreign compounds in biological systems 1 - 10 2026年1月査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Therapeutic potential of pycnogenol: antioxidant, anti-inflammatory, immunomodulatory, antiviral, and anticancer effects. . Frontiers in pharmacology17 1755175 2026年査読国際誌

Akhter R, Kitab B, Kayesh MEH, Shimizu R, Onuma H, Yamamoto N, Ogawa S, Sugiyama M, Tanaka Y, Sato Y, Kohara M, Tsukiyama-Kohara K . Optimization of lipid nanoparticles loaded with ribonucleoprotein-oligonucleotide complexes for in vivo delivery of a CRISPR/Cas9 system targeting hepatitis B virus. . Virus research 199682 2025年12月査読国際誌

Kayesh MEH, Sanada T, Kohara M, Tsukiyama-Kohara K . Natural Hepacivirus Infection in Tree Shrews: A Call for Routine Screening in Hepatitis Virus Research. . Viruses18 ( 1 ) 2025年12月査読国際誌

Kitab B., Kohara M., Tsukiyama-Kohara K. . Ribonucleotide reductase subunit M2 mediates the mTOR pathway to recruit furin endoprotease and promote maturation of dengue virus . Iscience28 ( 12 ) 2025年12月査読国際誌

担当区分：筆頭著者,　最終著者,　責任著者記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Iscience

Dengue virus (DENV) maturation involves calcium-dependent endoprotease furin, which cleaves the precursor membrane (prM) into the M protein in the trans-Golgi network, facilitating the release of infectious virus. Here, we demonstrate that ribonucleotide reductase subunit M2 (RRM2) recruits furin to mediate the cleavage of the DENV prM protein. Silencing of RRM2 reduced furin expression, leading to the accumulation of uncleaved prM in infected cells, increased intracellular DENV RNA, and diminished infectious virus titers in the supernatant. DENV infection prompted colocalization and interaction between RRM2 and furin in hepatoma cells and liver tissues of infected mice, with enhancement of RRM2 expression and furin stability. Silencing RRM2 impeded the effects of mammalian target of rapamycin (mTOR), leading to decreased furin expression. Additionally, mTOR overexpression reduced uncleaved prM and intracellular viral RNA while increasing furin levels in DENV-infected cells. These findings highlighted a DENV maturation process, which is fine-tuned by the RRM2-mTOR-mediated pathway.

DOI： 10.1016/j.isci.2025.113998

Scopus

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Carboxy-vinyl polymer: A promising immunostimulant for vaccines targeting respiratory and internal organ viruses. . Vaccine72 128146 2025年12月査読国際誌

Akhter R, Hossain KA, Kitab B, Kayesh MEH, Tsukiyama-Kohara K . Comparison of the Regulatory Effects of Host Factors on Viral Internal Ribosomal Entry Sites. . Veterinary sciences12 ( 12 ) 2025年11月査読国際誌

Hossain KA, Kitab B, Kono R, Momohara K, Akhter R, Rashid MHO, Soliman AM, Hifumi T, Tun MMN, Kaesh MEH, Morita K, Kohara M, Tsukiyama-Kohara K . Roles of non-structural protein 1 in dengue virus replication and immunomodulation in vivo. . The Journal of veterinary medical science 2025年11月査読国際誌

Soliman AM, Mahmoud HYAH, Amer MM, Mohamed S, Hifumi T, Tsukiyama-Kohara K, Tanaka T . Molecular detection and phylogenetic characterization of Borrelia theileri and a putative novel Borrelia genotype in Hyalomma dromedarii ticks infesting camels in southern Egypt. . Comparative immunology, microbiology and infectious diseases124 102403 2025年9月査読国際誌

Soliman AM, Mahmoud HYAH, Amer MM, Mohamed S, Hifumi T, Tsukiyama-Kohara K, Tanaka T . First detection of Rickettsia japonica in Hyalomma dromedarii ticks infesting camels from Egypt: A call for enhanced surveillance of tick-borne pathogens. . Medical and veterinary entomology 2025年9月査読国際誌

Akter L, Hashem MA, Kayesh MEH, Rakib TM, Rashid MHO, Maetani F, Tsukiyama-Kohara K . Correction: Elevated oncogene expressions in koala infected with multiple koala retrovirus subtypes: a preliminary study. . Virus genes 2025年8月査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Toll-like Receptor (TLR) Response in Chikungunya Virus Infection: Mechanism of Activation, Immune Evasion, and Use of TLR Agonists in Vaccine Development. . Vaccines13 ( 8 ) 2025年8月査読国際誌

Kayesh M.E.H., Kohara M., Tsukiyama-Kohara K. . Innate Immune Response to Powassan Virus Infection: Progress Toward Infection Control . Vaccines13 ( 7 ) 2025年7月査読国際誌

担当区分：筆頭著者,　最終著者,　責任著者記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Vaccines

Powassan virus is an emerging tick-borne flavivirus that poses a significant threat to human health. The outcome of Powassan virus infection is shaped by both viral factors and the host immune response. While this review aimed to examine the innate immune response, particularly toll-like receptor-mediated immune responses to Powassan virus, data specific to the immune response to Powassan virus remain scarce. Therefore, we focused on toll-like receptor responses to related flaviviruses to infer possible mechanisms of host response. Insights from both in vivo and in vitro studies are critical for guiding the development of effective therapeutic and preventive strategies. Currently, there are no clinically approved treatments or vaccines for Powassan virus, highlighting the urgent need for their development. We also highlight recent progress in POWV vaccine development, with an emphasis on the potential use of toll-like receptor agonists as adjuvants to enhance immunogenicity and improve vaccine efficacy.

DOI： 10.3390/vaccines13070754

Scopus

PubMed

Akter L, Hashem MA, Kayesh MEH, Rakib TM, Rashid MHO, Maetani F, Kohara KT . Elevated oncogene expressions in koala infected with multiple koala retrovirus subtypes: a preliminary study. . Virus genes 2025年6月査読国際誌

Sanada T, Tsukiyama-Kohara K, Kohara M . Genomic characterization of hepaciviruses and pegivirus in the northern tree shrew (Tupaia belangeri). . Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 105778 2025年6月査読国際誌

Rashid MHO, Ezzikouri S, Soliman AM, Akter L, Momohara K, Hifumi T, Miyoshi N, Hishiki T, Abdel-Moneim AS, Kohara M, Tsukiyama-Kohara K . Drug repositioning: Identification of potent inhibitors of NS3 protease and NS5 RdRp for control of DENV infection. . Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie187 118104 2025年4月査読国際誌

Rashid MHO, Yasui F, Sanada T, Kono R, Honda T, Kitab B, Akter L, Utsunomiya M, Sato R, Yoshida O, Hiasa Y, Oda Y, Goh Y, Miyazaki T, Kohara M, Tsukiyama-Kohara K . Immunogenicity of an Intranasal Dual (Core and Surface)-Antigen Vaccine Against Hepatitis B Virus Enhanced by Carboxyl-Vinyl Polymer Excipients. . Vaccines13 ( 5 ) 2025年4月査読国際誌

Kayesh MEH, Kohara M, Tsukiyama-Kohara K . Effects of oxidative stress on viral infections: an overview. . Npj viruses3 ( 1 ) 27 2025年4月査読国際誌

Akhter R, Hossain KA, Kitab B, Sakoda Y, Tsukiyama-Kohara K . Common host factors for internal ribosomal entry site-mediated translation of viral genomic RNA: An investigation in foot-and-mouth disease and classical swine fever viruses. . Virus research 199570 2025年4月査読国際誌

Uno Y, Kawabata I, Ushirozako G, Tsukiyama-Kohara K, Ishizuka M, Mizukawa H, Murayama N, Yamazaki H . Exploration of functional cytochrome P450 4F enzymes in liver, intestine, and kidney from dogs, cats, pigs, and tree shrews and comparison of their metabolic capacities with human P450 4F2 and 4F12. . Biochemical pharmacology 116894 2025年3月査読国際誌

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