Faculty Profiles - Kyoko (Tsukiyama-)Kohara

Papers - Kyoko (Tsukiyama-)Kohara

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Elmessaoudi-Idrissi M, Tsukiyama-Kohara K, Nourlil J, Kettani A, Windisch MP, Kohara M, Malik YS, Dhama K, Benjelloun S, Ezzikouri S. . Structure-guided discovery approach identifies potential lead compounds targeting M pro of SARS-CoV-2 . Virusdisease 2020.11Reviewed

Sayeh Ezzikouri, Jalal Nourlil, Kyoko Tsukiyama-Kohara, Michinori Kohara, Hicham El Ossmani, Marc P Windisch, Soumaya Benjelloun . Nanobodies: an unexplored opportunity to combat COVID-19. . Journal of biomolecular structure & dynamics 1 - 3 2020.11Reviewed International journal

Language：English Publishing type：Research paper (scientific journal)

Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory coronavirus 2 (SARS-CoV-2). This virus is capable of human-to-human transmission, and is spreading rapidly round the globe, with markedly high fatality rates. Unfortunately, there are neither vaccines nor specific therapies available to combat it, and the developments of such approaches depend on pursuing multiple avenues in biomedical science. Accordingly, in this paper we highlight one such avenue-nanobodies-for potential utility in therapeutic and diagnostic interventions to combat COVID-19.Communicated by Ramaswamy H. Sarma.

DOI： 10.1080/07391102.2020.1845801

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Kayesh MEH, Hashem MA, Tsukiyama-Kohara K. . Koala retrovirus epidemiology, transmission mode, pathogenesis, and host immune response in koalas (Phascolarctos cinereus): a review. . Arch Virol. 165 ( 11 ) 2409 - 2417 2020.11Invited Reviewed

Ezzikouri S, Nourlil J, Tsukiyama-Kohara K, Kohara M, El Ossmani H, Windisch MP, Benjelloun S. . Nanobodies: an unexplored opportunity to combat COVID-19 . J Biomol Struct Dyn.10 1 - 3 2020.11Reviewed

Mohammad Enamul Hoque Kayesh, Md Abul Hashem, Kyoko Tsukiyama-Kohara . Koala retrovirus epidemiology, transmission mode, pathogenesis, and host immune response in koalas (Phascolarctos cinereus): a review. . Archives of virology165 ( 11 ) 2409 - 2417 2020.11Reviewed International journal

Language：English Publishing type：Research paper (scientific journal)

Koala retrovirus (KoRV) is a major threat to koala health and conservation. It also represents a series of challenges across the fields of virology, immunology, and epidemiology that are of great potential interest to any researcher in the field of retroviral diseases. KoRV is a gammaretrovirus that is present in both endogenous and exogenous forms in koala populations, with a still-active endogenization process. KoRV may induce immunosuppression and neoplastic conditions such as lymphoma and leukemia and play a role in chlamydiosis and other diseases in koalas. KoRV transmission modes, pathogenesis, and host immune response still remain unclear, and a clear understanding of these areas is critical for devising effective preventative and therapeutic strategies. Research on KoRV is clearly critical for koala conservation. In this review, we provide an overview of the current understanding and future challenges related to KoRV epidemiology, transmission mode, pathogenesis, and host immune response and discuss prospects for therapeutic and preventive vaccines.

DOI： 10.1007/s00705-020-04770-9

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Hashem MA, Maetani F, Kayesh MEH, Eiei T, Mochizuki K, Ito A, Sakurai H, Asai T, Tsukiyama-Kohara K. . Transmission of koala retrovirus from parent koalas to a joey in a Japanese zoo. . Journal of Virolgy94 ( 11 ) e0019-20 2020.5Reviewed

Chi Hai-Ying, Yuuki Tanaka, Tatsuro Hifumi, Ko-ichiro Shoji, Mohammad Enamul Hoque Kayesh, Md Abul Hashem, Bouchra Kitab, Takahiro Sanada, Tomoko Fujiyuki, Misako Yoneda, Hitoshi Hatai, Akira Yabuki, Noriaki Miyoshi, Chieko Kai, Michinori Kohara, Kyoko Tsukiyama-Kohara . Pathological and genetic aspects of spontaneous mammary gland tumor in Tupaia belangeri (Tree shrew) . PLoS One15 ( 5 ) e0233232 2020.5Reviewed

Sayeh Ezzikouri, Mohammad Enamul Hoque Kayesh, Soumaya Benjelloun, Michinori Kohara and Kyoko Tsukiyama-Kohara . Targeting host innate and adaptive immunity to achieve the functional cure of chronic hepatitis B . Vaccines8 ( 2 ) E216 2020.5Reviewed

Mohammad Enamul Hoque Kayesh 1,2 , Md Abul Hashem 1, Bouchra Kitab 1 and Kyoko Tsukiyama-Kohara . Pathogenesis and Immune Response Caused by Vector-Borne and Other Viral Infections in a Tupaia Model . microorganism 2019.12Invited Reviewed

Sanada T, Yamamoto N, Kayesh MEH, Tsukiyama-Kohara K, Hasegawa H, Miyazaki T, Takano JI, Shiogama Y, Yasutomi Y, Goh Y, Yoshida O, Hiasa Y, Kohara M. . Intranasal vaccination with HBs and HBc protein combined with carboxyl vinyl polymer induces strong neutralizing antibody, anti-HBs IgA, and IFNG response. . Biochem Biophys Res Commun.520 ( 1 ) 86 - 92 2019.11Reviewed

Takahiro Sanada, Naoki Yamamoto, Mohammad Enamul Hoque Kayesh, Kyoko Tsukiyama-Kohara, Hideki Hasegawa, Takashi Miyazaki, Jun-Ichiro Takano, Yumiko Shiogama, Yasuhiro Yasutomi, Yasumasa Goh, Osamu Yoshida, Yoichi Hiasa, Michinori Kohara . Intranasal vaccination with HBs and HBc protein combined with carboxyl vinyl polymer induces strong neutralizing antibody, anti-HBs IgA, and IFNG response. . Biochemical and biophysical research communications520 ( 1 ) 86 - 92 2019.11Reviewed International journal

Language：English Publishing type：Research paper (scientific journal)

Hepatitis B virus (HBV) infection causes acute and chronic hepatitis, which is a major public health concern worldwide. Immunization methods incorporating hepatitis B surface-small (HBs-S) antigen and hepatitis B core antigen (HBc) have been proposed as candidate therapeutic vaccines, but the elimination of existing HBV infection remains a challenge. To enhance the efficacy of HBs and HBc vaccination, we investigated HBs-large (HBs-L) as an immunogen, and carboxyl vinyl polymer (CVP) as an excipient. HBs-S or HBs-L, in combination with HBc antigen, was administered subcutaneously (without CVP) or intranasally (with or without CVP) for the evaluation of immune response in the tree shrew, which is considered to be a suitable small animal model of HBV infection. Immunization with HBs-L antigen by either route induced a rapid IgG response. Intranasal immunization with HBs-S or HBs-L and HBc formulated with CVP strongly induced neutralizing antibody activity, IgA response, and HBc-specific expression of the interferon gamma-encoding gene. These data indicated the potential of HBs-L and HBc intranasal immunization with CVP, not only as a therapeutic vaccine, but also as a prophylactic vaccine candidate.

DOI： 10.1016/j.bbrc.2019.09.072

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Hashem MA, Kayesh MEH, Yamato O, Maetani F, Eiei T, Mochizuki K, Sakurai H, Ito A, Kannno H, Kasahara T, Amano Y, Tsukiyama-Kohara K. . Coinfection with koala retrovirus subtypes A and B and its impact on captive koalas in Japanese zoos. . Arch Virol. 164 ( 11 ) 2735 - 2745 2019.11Reviewed

Md Abul Hashem, Mohammad Enamul Hoque Kayesh, Osamu Yamato, Fumie Maetani, Taiki Eiei, Kyoya Mochizuki, Hiroko Sakurai, Ayaka Ito, Hiroki Kannno, Tatsuya Kasahara, Yusuke Amano, Kyoko Tsukiyama-Kohara . Coinfection with koala retrovirus subtypes A and B and its impact on captive koalas in Japanese zoos. . Archives of virology164 ( 11 ) 2735 - 2745 2019.11Reviewed International journal

Language：English Publishing type：Research paper (scientific journal)

Koala retrovirus (KoRV) is unique among endogenous retroviruses because its endogenization is still active. Two major KoRV subtypes, KoRV-A and B, have been described, and KoRV-B is associated with disease and poses a health threat to koalas. Here, we investigated the co-prevalence of KoRV-A and KoRV-B, detected by type-specific PCR and sequencing, and their impact on the health of koalas in three Japanese zoos. We also investigated KoRV proviral loads and found varying amounts of genomic DNA (gDNA) in peripheral blood mononuclear cells (PBMCs). We found that 100% of the koalas examined were infected with KoRV-A and 60% (12/20) were coinfected with KoRV-B. The KoRV-A sequence was highly conserved, whereas the KoRV-B sequence varied among individuals. Interestingly, we observed possible vertical transmission of KoRV-B in one offspring in which the KoRV-B sequence was similar to that of the father but not the mother. Moreover, we characterized the KoRV growth patterns in concanavalin-A-stimulated PBMCs isolated from KoRV-B-coinfected or KoRV-B-uninfected koalas. We quantified the KoRV provirus in gDNA and the KoRV RNA copy numbers in cells and culture supernatants by real-time PCR at days 4, 7, and 14 post-seeding. As the study population is housed in captivity, a longitudinal study of these koalas may provide an opportunity to study the transmission mode of KoRV-B. In addition, we characterized KoRV isolates by infecting tupaia cells. The results suggested that tupaia may be used as an infection model for KoRV. Thus, this study may enhance our understanding of KoRV-B coinfection and transmission in the captive koalas.

DOI： 10.1007/s00705-019-04392-w

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Sanada T, Tsukiyama-Kohara K, Shin-I T, Yamamoto N, Kayesh MEH, Yamane D, Takano JI, Shiogama Y, Yasutomi Y, Ikeo K, Gojobori T, Mizokami M, Kohara M. . Construction of complete Tupaia belangeri transcriptome database by whole-genome and comprehensive RNA sequencing. . Sci Rep. 9 ( 1 ) 12372 2019.8Reviewed

Takahiro Sanada, Kyoko Tsukiyama-Kohara, Tadasu Shin-I, Naoki Yamamoto, Mohammad Enamul Hoque Kayesh, Daisuke Yamane, Jun-Ichiro Takano, Yumiko Shiogama, Yasuhiro Yasutomi, Kazuho Ikeo, Takashi Gojobori, Masashi Mizokami, Michinori Kohara . Construction of complete Tupaia belangeri transcriptome database by whole-genome and comprehensive RNA sequencing. . Scientific reports9 ( 1 ) 12372 - 12372 2019.8Reviewed International journal

Language：English Publishing type：Research paper (scientific journal)

The northern tree shrew (Tupaia belangeri) possesses high potential as an animal model of human diseases and biology, given its genetic similarity to primates. Although genetic information on the tree shrew has already been published, some of the entire coding sequences (CDSs) of tree shrew genes remained incomplete, and the reliability of these CDSs remained difficult to determine. To improve the determination of tree shrew CDSs, we performed sequencing of the whole-genome, mRNA, and total RNA and integrated the resulting data. Additionally, we established criteria for the selection of reliable CDSs and annotated these sequences by comparison to the human transcriptome, resulting in the identification of complete CDSs for 12,612 tree shrew genes and yielding a more accurate tree shrew genome database (TupaiaBase: http://tupaiabase.org ). Transcriptome profiles in hepatitis B virus infected tree shrew livers were analyzed for validation. Gene ontology analysis showed enriched transcriptional regulation at 1 day post-infection, namely in the "type I interferon signaling pathway". Moreover, a negative regulator of type I interferon, SOCS3, was induced. This work, which provides a tree shrew CDS database based on genomic DNA and RNA sequencing, is expected to serve as a powerful tool for further development of the tree shrew model.

DOI： 10.1038/s41598-019-48867-x

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Matsui T, Handa Y, Kanda T, Tsukiyama-Kohara K. . Silencing of the foot-and-mouth disease virus internal ribosomal entry site by targeting relatively conserved region among serotypes. . Virus Genes. 2019.7Reviewed

Kitab B, Satoh M, Ohmori Y, Munakata T, Sudoh M, Kohara M, Tsukiyama-Kohara K . Ribonucleotide reductase M2 promotes RNA replication of hepatitis C virus by protecting NS5B protein from hPLIC1-dependent proteasomal degradation. . J. Biol. Chem.294 ( 15 ) 5759 - 5773 2019.4Reviewed

Sanada T, Yasui F, Honda T, Kayesh MEH, Takano JI, Shiogama Y, Yasutomi Y, Tsukiyama-Kohara K, Kohara M. . Avian H5N1 influenza virus infection causes severe pneumonia in the Northern tree shrew (Tupaia belangeri). . Virology529 101 - 110 2019.3Reviewed

Kayesh MEH, Yamato O, Rahman MM, Hashem MA, Maetani F, Eiei T, Mochizuki K, Sakurai H, Tsukiyama-Kohara K. . Molecular dynamics of koala retrovirus infection in captive koalas in Japan. . Arch. Virol.164 ( 3 ) 757 - 765 2019.3Reviewed

Takahiro Sanada, Fumihiko Yasui, Tomoko Honda, Mohammad Enamul Hoque Kayesh, Jun-Ichiro Takano, Yumiko Shiogama, Yasuhiro Yasutomi, Kyoko Tsukiyama-Kohara, Michinori Kohara . Avian H5N1 influenza virus infection causes severe pneumonia in the Northern tree shrew (Tupaia belangeri). . Virology529 101 - 110 2019.3Reviewed International journal

Language：English Publishing type：Research paper (scientific journal)

Avian-origin influenza viruses like H5N1 and H7N9 often cause severe symptoms with high mortality in humans. Animal models are useful for clarification of the mechanisms of pathogenicity of these infections. In this study, to expand the potential utility of the Northern tree shrew (Tupaia belangeri) for influenza virus infection, we assessed the pathogenicity of H5N1 and H7N9 avian influenza viruses in tupaia. Infectious virus was detected continuously from nasal, oral, tracheal, and conjunctival swab samples in the animals infected with these viruses. H5N1 influenza virus infection of tupaia caused severe diffuse pneumonia with fever and weight loss. In contrast, H7N9 influenza virus infection caused focal pneumonia. The severity of pneumonia was correlated with proinflammatory cytokine transcript levels. These results indicated that tupaia can be another suitable animal model for avian influenza virus research.

DOI： 10.1016/j.virol.2019.01.015

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Papers - Kyoko (Tsukiyama-)Kohara