研究者詳細 - 二神　泰基

論文 - 二神　泰基

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Futagami T, Kadooka C, Ando Y, Okutsu K, Yoshizaki Y, Setoguchi S, Takamine K, Kawai M, Tamaki H. . Multi-gene phylogenetic analysis reveals that shochu-fermenting Saccharomyces cerevisiae strains form a distinct sub-clade of the Japanese sake cluster . Yeast 2017年7月査読

Rahayu YY, Yoshizaki Y, Yamaguchi K, Okutsu K, Futagami T, Tamaki H, Sameshima Y, Takamine K. . Key volatile compounds in red koji-shochu, a Monascus-fermented product, and their formation steps during fermentation . Food Chemistry 2017年6月査読

Rahayu YY, Yoshizaki Y, Yamaguchi K, Okutsu K, Futagami T, Tamaki H, Sameshima Y, Takamine K . Key volatile compounds in red koji-shochu, a Monascus-fermented product, and their formation steps during fermentation. . Food chemistry224 398 - 406 2017年6月

Suenaga H, Yamazoe A, Hosoyama A, Kimura N, Hirose J, Watanabe T, Fujihara H, Futagami T, Goto M, Furukawa K . Complete Genome Sequence of the Polychlorinated Biphenyl-Degrading Bacterium Pseudomonas putida KF715 (NBRC 110667) Isolated from Biphenyl-Contaminated Soil. . Genome announcements5 ( 7 ) 2017年2月

Suenaga H, Yamazoe A, Hosoyama A, Kimura N, Hirose J, Watanabe T, Fujihara H, Futagami T, Goto M, Furukawa K. . Complete genome sequence of the polychlorinated biphenyl-degrading bacterium Pseudomonas putida KF715 (NBRC 110667) isolated from biphenyl-contaminated soil . Genome Announc. 2017年2月

奥津果優、門岡千尋、小城章裕、吉﨑由美子、二神泰基、玉置尚徳、髙峯和則. . 中国及び韓国の市場品「神麹」における菌叢と含有成分の実態調査 . 生薬学雑誌 2017年2月

奥津果優, 門岡千尋, 小城章裕, 吉崎由美子, 二神泰基, 玉置尚徳, 高峯和則 . 中国及び韓国の市場品「神麹」における菌叢と含有成分の実態調査 . 生薬学雑誌71 ( 1 ) 41 - 48 2017年2月

出版者・発行元：(一社)日本生薬学会

中国および韓国で入手した市場品の神麹15種をサンプルとし、菌叢、酵素活性(アミラーゼ、リパーゼ、プロテアーゼ)およびフェルラ酸含量について調査した。Denaturing gradient gel electrophoresis(DGGE)解析の結果、神麹には乳酸菌や枯草菌など複数の微生物が存在し、製品によって微生物の種類が大きく異なることが判明した。また、半数以上のサンプルでアミラーゼ活性およびリパーゼ活性を認め、全サンプルでフェルラ酸の含有を認めた。色調により暗褐色系、白色系、黄褐色系の3群に分類して主成分分析した結果、酵素活性が検出されなかったのは全て黄褐色群で、明度が低いほどフェルラ酸含量が高い傾向であった。以上より、神麹は、色調によって酵素活性およびフェルラ酸含量の予測が可能であると考えられた。

Kojo T, Kadooka C, Komohara M, Onitsuka S, Tanimura M, Muroi Y, Kurazono S, Shiraishi Y, Oda K, Iwashita K, Onoue M, Okutsu K, Yoshizaki Y, Takamine K, Futagami T, Mori K, Tamaki H . Characterization of amylolytic enzyme overproducing mutant of Aspergillus luchuensis obtained by ion beam mutagenesis . The Journal of General and Applied Microbiology 2017年査読

奥津果優, 門岡千尋, 小城章裕, 吉﨑由美子, 二神泰基, 玉置尚徳, 髙峯和則 . 中国及び韓国の市場品「神麹」における菌叢と含有成分の実態調査 . 生薬学雑誌71 ( 1 ) 41 - 48 2017年

出版者・発行元：一般社団法人日本生薬学会

"Shinkiku" is a traditional digestive drug prepared by the fermentation of wheat and some herbs with fermentative microbes. Shinkiku is manufactured in China and Korea, and also used in Japanese Kampo medicine as a component of Hangebyakujutsutemmato. However, there are currently no quality standards for shinkiku, and thus, the quality of shinkiku has considerable variation depending on its manufacturer. Although these variations would be partially derived from the differences in fermentative microbes, there are no studies about microbial diversities or chemical constituents in commercial shinkiku. Thus, we investigated the microbial diversity and chemical constituents of 15 commercial shinkiku samples to standardize its quality. PCR-denaturing gradient gel electrophoresis of 16S rDNA and ITS1 sequences revealed that different microbes such as Lactobacillus sp. and Candida sp. were present in each shinkiku sample. On the other hand, most shinkiku samples showed amylase (12/15 samples) and lipase activities (9/15 samples) that behave as digestants. In addition, all samples commonly contained ferulic acid (>10 nmol/g), which has anti-inflammatory and anti-oxidant activities. Thus, enzyme activities and ferulic acid were suggested to be one of the candidates for use as reference standards for the quality control of shinkiku. Exceptional shinkiku samples without enzyme activities showed a baked brown color, and ferulic acid content was inversely related with the brightness color of shinkiku (R2=0.47). Therefore, it seems that color indices would be effective to predict the quality of shinkiku such as enzyme activities and ferulic acid.

DOI： 10.24684/jspharm.71.1_41

CiNii Research

Osamu Yamada, Masayuki Machida, Akira Hosoyama, Masatoshi Goto, Toru Takahashi, Taiki Futagami, Youhei Yamagata, Michio Takeuchi, Tesuo Kobayashi, Hideaki Koike, Keietsu Abe, Kiyoshi Asai, Masanori Arita, Nobuyuki Fujita, Kazurou Fukuda, Kenichi Higa, Hiroshi Horikawa, Takeaki Ishikawa, Koji Jinno, Yumiko Kato, Kohtaro Kirimura, Osamu Mizutani, Kaoru Nakasone, Motoaki Sano, Yohei Shiraishi, Masatoshi Tsukahara, and Katsuya Gomi . Genome sequence of Aspergillus luchuensis NBRC 4314 . DNA research00 ( 0 ) 1 - 9 2016年9月査読

Yohei Shiraishi, Yumiko Yoshizaki, Toshifumi Ono, Hiroaki Yamato, Kayu Okutsu, Hisanori Tamaki, Taiki Futagami, Sameshima Yoshihiro, and Kazunori Takamine . Characteristic odour compounds in shochu derived from rice koji . Journal of the Institute of Brewing122 ( 3 ) 381 - 387 2016年7月査読

Kadooka C, Onitsuka S, Uzawa M, Tashiro S, Kajiwara Y, Takashita H, Okutsu K, Yoshizaki Y, Takamine K, Goto M, Tamaki H, Futagami T. . Marker recycling system using the sC gene in the white koji mold, Aspergillus luchuensis mut. kawachii . The Journal of General and Applied Microbiology62 ( 3 ) 160 - 163 2016年5月査読

Fujihara H, Yamazoe A, Hosoyama A, Suenaga H, Kimura N, Hirose J, Watanabe T, Futagami T, Goto M, Furukawa K. . Draft Genome Sequence of Pseudomonas abietaniphila KF701 (NBRC110664), a Polychlorinated Biphenyl-Degrading Bacterium Isolated from Biphenyl-Contaminated Soil. . Genome Announc.3 ( 3 ) e00473-15 2015年5月査読

Fujihara H, Yamazoe A, Hosoyama A, Suenaga H, Kimura N, Hirose J, Watanabe T, Futagami T, Goto M, Furukawa K. . Draft Genome Sequence of Pseudomonas aeruginosa KF702 (NBRC 110665), a Polychlorinated Biphenyl-Degrading Bacterium Isolated from Biphenyl-Contaminated Soil. . Genome Announc.3 ( 3 ) e00517-15. 2015年5月査読

Kimura N, Yamazoe A, Hosoyama A, Hirose J, Watanabe T, Suenaga H, Fujihara H, Futagami T, Goto M, Furukawa K. . Draft Genome Sequence of Pseudomonas abietaniphila KF717 (NBRC 110669), Isolated from Biphenyl-Contaminated Soil in Japan. . Genome Announc. e00059-15 2015年3月査読

Watanabe T, Yamazoe A, Hosoyama A, Fujihara H, Suenaga H, Hirose J, Futagami T, Goto M, Kimura N, Furukawa K. . Draft Genome Sequence of Cupriavidus pauculus Strain KF709, a Biphenyl-Utilizing Bacterium Isolated from Biphenyl-Contaminated Soil. . Genome Announc. doi: 10.1128/genomeA.00222-15. 2015年3月査読

Suenaga H, Yamazoe A, Hosoyama A, Kimura N, Hirose J, Watanabe T, Fujihara H, Futagami T, Goto M, Furukawa K. . Draft Genome Sequence of the Polychlorinated Biphenyl-Degrading Bacterium Cupriavidus basilensis KF708 (NBRC 110671) Isolated from Biphenyl-Contaminated Soil. . Genome Announc. e00143-15 2015年3月査読

Suenaga H, Yamazoe A, Hosoyama A, Kimura N, Hirose J, Watanabe T, Fujihara H, Futagami T, Goto M, Furukawa K. . Draft Genome Sequence of the Polychlorinated Biphenyl-Degrading Bacterium Pseudomonas putida KF703 (NBRC 110666) Isolated from Biphenyl-Contaminated Soil. . Genome Announc. e00142-15. 2015年3月査読

Futagami T, Seto K, Kajiwara Y, Takashita H, Omori T, Takegawa K, Goto M. . The putative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans. . Biosci. Biotechnol. Biochem.78 2014年4月査読

Kawai M, Futagami T, Toyoda A, Takaki Y, Nishi S, Hori S, Arai W, Tsubouchi T, Morono Y, Uchiyama I, Itoh T, Fujiyama A, Inagaki F, Takami H. . High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes. . Front. Microbiol.5 80 2014年3月査読

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