Updated on 2023/01/24

写真a

 
OKUYA Kosuke
 
Organization
Research Field in Veterinary Medicine, Agriculture, Fisheries and Veterinary Medicine Area Joint faculty of Veterinary Medicine Transboundary Animal Disease Research Center Assistant Professor
Title
Assistant Professor
External link

Degree

  • Ph.D. ( 2021.3   Hokkaido University )

Research Interests

  • 動物衛生学

  • ウイルス学

Research Areas

  • Life Science / Veterinary medical science

  • Life Science / Virology

Education

  • Hokkaido University

    2017.4 - 2021.3

  • Kagoshima University   Faculty of Agriculture   Veterinary Medicine

    2010.4 - 2017.3

Research History

  • Kagoshima University   Assistant Professor

    2022.1

  • Kagoshima University   Joint faculty of Veterinary Medicine Transboundary Animal Disease Research Center   Assistant Professor

    2021.6

  • Hokkaido University   International Institute for Zoonosis Control

    2021.4 - 2021.5

  • Japan Society for the Promotion of Science

    2018.4 - 2021.3

  • University of Wisconsin-Madison   Influenza Research Institute   Honorary Fellow

    2015.3 - 2016.2

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    Country:United States

Professional Memberships

  • 日本ウイルス学会

    2018

  • 日本獣医学会

    2013.9

Committee Memberships

  • KAICO株式会社   アドバイザー  

    2022.9   

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    Committee type:Other

Studying abroad experiences

  • 2019.5 - 2019.7   世界保健機関 (WHO) ベトナムオフィス  

  • 2015.3 - 2016.2   University of Wisconsin-Madison   Honorary fellow

Qualification acquired

  • Zoonosis Control Expert (ZCE)

  • Veterinarian

  • JGAP指導員

 

Papers

  • Matsunaga N, Ijiri M, Ishikawa K, Ozawa M, Okuya K, Khalil AM, Kojima I, Esaki M, Masatani T, Matsui T, Fujimoto Y .  Avian paramyxovirus serotype-1 isolation from migratory birds and environmental water in southern Japan: an epidemiological survey during the 2018/19-2021/2022 winter seasons. .  Microbiology and immunology   2023.1Avian paramyxovirus serotype-1 isolation from migratory birds and environmental water in southern Japan: an epidemiological survey during the 2018/19-2021/2022 winter seasons.Reviewed International coauthorship International journal

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    DOI: 10.1111/1348-0421.13053

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  • Mitarai S, Okuya K, Miyane K, Miyamoto M, Ishikawa S, Kawaguchi H, Hatazoe I, Suda Y, Arima E, Nakazato H, Hobo S, Masatani T, Ozawa M .  Genetic characterization of bovine respiratory syncytial viruses in Japan between 2017 and 2019. .  Archives of virology168 ( 2 ) 51   2023.1Reviewed International coauthorship International journal

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    Authorship:Lead author   Language:English   Publisher:Archives of Virology  

    Bovine respiratory syncytial virus (BRSV) strains that were detected in Kagoshima prefecture and isolated in Hokkaido between 2017 and 2019, together with a BRSV vaccine strain, were subjected to full-genome sequencing. The BRSV strains identified in Japan were found to be genetically close to each other but distant from the vaccine strains. The deduced amino acids at positions 206 and 208 of the glycoprotein (G protein), which form one of the major epitopes of the recent Japanese BRSV strains, were different from those of the vaccine strains. Therefore, the recent Japanese BRSV strains might be antigenically different from the BRSV vaccine strains.

    DOI: 10.1007/s00705-022-05670-w

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  • Kajiya T, Sawayama H, Arima E, Okamoto M, Baba M, Toyama M, Okuya K, Ozawa M, Atsuchi N, Nishi J, Suda Y .  Novel RT-PCR Using Sugar Chain-Immobilized Gold-Nanoparticles Correlates Patients' Symptoms: The Follow-Up Study of COVID-19 Hospitalized Patients. .  Viruses14 ( 11 )   2022.11Novel RT-PCR Using Sugar Chain-Immobilized Gold-Nanoparticles Correlates Patients' Symptoms: The Follow-Up Study of COVID-19 Hospitalized Patients.Reviewed International coauthorship International journal

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    DOI: 10.3390/v14112577

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  • Fujimoto Y, Ogasawara K, Isoda N, Hatai H, Okuya K, Watanabe Y, Takada A, Sakoda Y, Saito K, Ozawa M .  Experimental and natural infections of white-tailed sea eagles (Haliaeetus albicilla) with high pathogenicity avian influenza virus of H5 subtype .  Frontiers in Microbiology13 ( 1007350 )   2022.10Experimental and natural infections of white-tailed sea eagles (Haliaeetus albicilla) with high pathogenicity avian influenza virus of H5 subtype Reviewed

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  • Kojima I, Onomoto K, Zuo W, Ozawa M, Okuya K, Naitou K, Izumi F, Okajima M, Fujiwara T, Ito N, Yoneyama M, Yamada K, Nishizono A, Sugiyama M, Fujita T, Masatani T .  The Amino Acid at Position 95 in the Matrix Protein of Rabies Virus Is Involved in Antiviral Stress Granule Formation in Infected Cells. .  Journal of virology   e0081022   2022.9The Amino Acid at Position 95 in the Matrix Protein of Rabies Virus Is Involved in Antiviral Stress Granule Formation in Infected Cells.Reviewed International coauthorship International journal

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    DOI: 10.1128/jvi.00810-22

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  • Okuya K, Khalil AM, Esaki M, Kojima I, Nishi N, Koyamada D, Matsui T, Yoshida Y, Ozawa M .  Genetic Characterization of Avian Influenza Viruses Isolated from the Izumi Plain, Japan in 2019/20 Winter Season. .  Pathogens (Basel, Switzerland)11 ( 9 )   2022.9Genetic Characterization of Avian Influenza Viruses Isolated from the Izumi Plain, Japan in 2019/20 Winter Season.Reviewed International coauthorship International journal

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    DOI: 10.3390/pathogens11091013

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  • Hattori T, Saito T, Okuya K, Takahashi Y, Miyamoto H, Kajihara M, Igarashi M, Takada A .  Human ACE2 Genetic Polymorphism Affecting SARS-CoV and SARS-CoV-2 Entry into Cells. .  Microbiology spectrum   e0087022   2022.7Human ACE2 Genetic Polymorphism Affecting SARS-CoV and SARS-CoV-2 Entry into Cells.Reviewed International coauthorship International journal

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    DOI: 10.1128/spectrum.00870-22

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  • Okuya K, Mine J, Tokorozaki K, Kojima I, Esaki M, Miyazawa K, Tsunekuni R, Sakuma S, Kumagai A, Takadate Y, Kikutani Y, Matsui T, Uchida Y, and Ozawa M .  Genetically Diverse Highly Pathogenic Avian Influenza A(H5N1/H5N8) Viruses among Wild Waterfowl and Domestic Poultry, Japan, 2021 .  Emerging Infectious Diseases28 ( 7 ) 1451 - 1455   2022.7Reviewed International coauthorship International journal

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  • Khalil A, Kojima I, Fukunaga W, Okajima M, Mitarai S, Fujimoto Y, Matsui T, Kuwahara M, Masatani T, Okuya K and Ozawa M .  Improved method for avian influenza virus isolation from environmental water samples .  Transboundary Emerging Diseases   2022.6Reviewed International coauthorship International journal

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  • Okuya K, Hattori T, Saito T, Takadate Y, Sasaki M, Furuyama W, Marzi A, Ohiro Y, Konno S, Hattori T, Takada A .  Multiple Routes of Antibody-Dependent Enhancement of SARS-CoV-2 Infection. .  Microbiology spectrum10 ( 2 ) e0155321   2022.3Reviewed International coauthorship International journal

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    Authorship:Lead author   Language:English   Publisher:Microbiology Spectrum  

    DOI: 10.1128/spectrum.01553-21

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  • Saito T, Hattori T, Okuya K, Manzoor R, Miyamoto H, Kajihara M, Takada A .  Molecular Mechanisms Underlying the Cellular Entry and Host Range Restriction of Lujo Virus. .  mBio13 ( 1 ) e0306021   2022.2Reviewed International coauthorship International journal

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    DOI: 10.1128/mbio.03060-21

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  • Seikai T, Takada A, Hasebe A, Kajihara M, Okuya K, Sekiguchi Yamada T, Kakuguchi W, Konno S, Ohiro Y .  Gargling with povidone iodine has a short-term inhibitory effect on SARS-Cov-2 in COVID-19 patients. .  The Journal of hospital infection123   179 - 181   2022.1Reviewed International coauthorship International journal

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    Authorship:Lead author   Language:English   Publisher:Journal of Hospital Infection  

    DOI: 10.1016/j.jhin.2022.01.001

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  • Funakoshi Kimitake, Maeda Fumikazu, Okuya Kosuke, Esaki Mana .  First record of the Endo’s pipistrelle, <i>Pipistrellus endoi</i>, in the Kyushu District, Japan: cranial characteristics and systematic positioning based on mitochondrial <i>CO1</i> sequences .  Honyurui Kagaku (Mammalian Science)62 ( 2 ) 239 - 245   2022First record of the Endo’s pipistrelle, <i>Pipistrellus endoi</i>, in the Kyushu District, Japan: cranial characteristics and systematic positioning based on mitochondrial <i>CO1</i> sequencesReviewed International coauthorship International journal

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    Authorship:Lead author   Language:Japanese   Publisher:The Mammal Society of Japan  

    <p>To date, Endo’s pipistrelle (<i>Pipistrellus endoi</i>) had not been recorded, in Kyushu District. To confirm the bat fauna, particularly whether <i>P. endoi</i> occurred, we performed a capture survey using mist nets in Yamaemura, Kumamoto Prefecture, in August 2021. Two bats were captured, one of which was identified as <i>Myotis macrodactylus</i>. The other bat which could not be identified was used as a specimen, which was considered to be either <i>P. abramus</i> or <i>P. endoi</i>. Measurements showed that the tragus of the specimen was wider than that of <i>P. abramus</i>. The upper first incisor was significantly longer than the upper second incisor. The upper second premolar of the specimen was larger than that of <i>P. abramus</i>, and the lower canine was smaller than that of <i>P. abramus</i>. The skull measurements of the specimen were the same as those of <i>P. endoi</i> in Honshu. The shape of the baculum of the specimen was similar to that of <i>P. endoi</i> in Honshu, and the length of the baculum was 9 mm, which was shorter than that of <i>P. abramus</i>. On the basis of these results, the specimen was identified as <i>P. endoi</i>. The results of principal component analysis for the 15 skull measurements of both species, including the present specimen, also indicated that the specimen was within the range of <i>P. endoi</i>, and skull shapes differed between the two species. Furthermore, phylogenetic analyses based on mitochondrial <i>CO1</i> sequences showed that the specimen belonged to a different genetic lineage than <i>P. abramus</i>.</p>

    DOI: 10.11238/mammalianscience.62.239

  • Fujimoto Y, Ogasawara K, Isoda N, Hatai H, Okuya K, Watanabe Y, Takada A, Sakoda Y, Saito K, Ozawa M .  Experimental and natural infections of white-tailed sea eagles (<i>Haliaeetus albicilla</i>) with high pathogenicity avian influenza virus of H5 subtype. .  Frontiers in microbiology13   1007350   2022Experimental and natural infections of white-tailed sea eagles (<i>Haliaeetus albicilla</i>) with high pathogenicity avian influenza virus of H5 subtype.Reviewed International coauthorship International journal

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    DOI: 10.3389/fmicb.2022.1007350

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  • Yurie Kida, Kosuke Okuya, Takeshi Saito, Junya Yamagishi, Aiko Ohnuma, Takanari Hattori, Hiroko Miyamoto, Rashid Manzoor, Reiko Yoshida, Naganori Nao, Masahiro Kajihara, Tokiko Watanabe, Ayato Takada .  Structural Requirements in the Hemagglutinin Cleavage Site-Coding RNA Region for the Generation of Highly Pathogenic Avian Influenza Virus .  Pathogens10 ( 12 )   2021.12Reviewed International coauthorship International journal

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    Highly pathogenic avian influenza viruses (HPAIVs) with H5 and H7 hemagglutinin (HA) subtypes are derived from their low pathogenic counterparts following the acquisition of multiple basic amino acids in their HA cleavage site. It has been suggested that consecutive adenine residues and a stem-loop structure in the viral RNA region that encodes the cleavage site are essential for the acquisition of the polybasic cleavage site. By using a reporter assay to detect non-templated nucleotide insertions, we found that insertions more frequently occurred in the RNA region (29 nucleotide-length) encoding the cleavage site of an H5 HA gene that was predicted to have a stem-loop structure containing consecutive adenines than in a mutated corresponding RNA region that had a disrupted loop structure with fewer adenines. In virus particles generated by using reverse genetics, nucleotide insertions that created additional codons for basic amino acids were found in the RNA region encoding the cleavage site of an H5 HA gene but not in the mutated RNA region. We confirmed the presence of virus clones with the ability to replicate without trypsin in a plaque assay and to cause lethal infection in chicks. These results demonstrate that the stem-loop structure containing consecutive adenines in HA genes is a key molecular determinant for the emergence of H5 HPAIVs.

    DOI: 10.3390/pathogens10121597

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  • Hayato Harima, Kosuke Okuya, Masahiro Kajihara, Hirohito Ogawa, Edgar Simulundu, Eugene Bwalya, Yongjin Qiu, Akina Mori-Kajihara, Musso Munyeme, Yoshihiro Sakoda, Takehiko Saito, Bernard M Hang'ombe, Hirofumi Sawa, Aaron S Mweene, Ayato Takada .  Serological and molecular epidemiological study on swine influenza in Zambia. .  Transboundary and emerging diseases69 ( 4 ) e931 - e943   2021.11Reviewed International coauthorship International journal

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    Influenza A viruses (IAVs) cause highly contagious respiratory diseases in humans and animals. In 2009, a swine-origin pandemic H1N1 IAV, designated A(H1N1)pdm09 virus, spread worldwide, and has since frequently been introduced into pig populations. Since novel reassortant IAVs with pandemic potential may emerge in pigs, surveillance for IAV in pigs is therefore necessary not only for the pig industry but also for public health. However, epidemiological information on IAV infection of pigs in Africa remains sparse. In this study, we collected 246 serum and 605 nasal swab samples from pigs in Zambia during the years 2011-2018. Serological analyses revealed that 49% and 32% of the sera collected in 2011 were positive for hemagglutination-inhibition (HI) and neutralizing antibodies against A(H1N1)pdm09 virus, respectively, whereas less than 5.3% of sera collected during the following period (2012-2018) were positive in both serological tests. The positive rate and the neutralization titers to A(H1N1)pdm09 virus were higher than those to classical swine H1N1 and H1N2 IAVs. On the other hand, the positive rate for swine H3N2 IAV was very low in the pig population in Zambia in 2011-2018 (5.3% and 0% in HI and neutralization tests, respectively). From nasal swab samples, we isolated one H3N2 and eight H1N1 IAV strains with an isolation rate of 1.5%. Phylogenetic analyses of all eight gene segments revealed that the isolated IAVs were closely related to human IAV strains belonging to A(H1N1)pdm09 and seasonal H3N2 lineages. Our findings indicate that reverse zoonotic transmission from humans to pigs occurred during the study period in Zambia and highlight the need for continued surveillance to monitor the status of IAVs circulating in swine populations in Africa. This article is protected by copyright. All rights reserved.

    DOI: 10.1111/tbed.14373

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  • Hayato Harima, Tony Schountz, Michihito Sasaki, Yasuko Orba, Kosuke Okuya, Yongjin Qiu, Christida E. Wastika, Katendi Changula, Masahiro Kajihara, Edgar Simulundu, Tomoyuki Yamaguchi, Yoshiki Eto, Akina Mori-Kajihara, Akihiko Sato, Satoshi Taniguchi, Ayato Takada, Masayuki Saijo, Bernard M. Hang’ombe, Hirofumi Sawa .  Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia .  PLOS Neglected Tropical Diseases15 ( 9 ) e0009768   2021.9Reviewed International coauthorship International journal

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    BACKGROUND: Pteropine orthoreovirus (PRV) is an emerging bat-borne zoonotic virus that causes severe respiratory illness in humans. Although PRVs have been identified in fruit bats and humans in Australia and Asia, little is known about the prevalence of PRV infection in Africa. Therefore, this study performed an PRV surveillance in fruit bats in Zambia. METHODS: Egyptian fruit bats (Rousettus aegyptiacus, n = 47) and straw-colored fruit bats (Eidolon helvum, n = 33) captured in Zambia in 2017-2018 were screened for PRV infection using RT-PCR and serum neutralization tests. The complete genome sequence of an isolated PRV strain was determined by next generation sequencing and subjected to BLAST and phylogenetic analyses. Replication capacity and pathogenicity of the strain were investigated using Vero E6 cell cultures and BALB/c mice, respectively. RESULTS: An PRV strain, tentatively named Nachunsulwe-57, was isolated from one Egyptian fruit bat. Serological assays demonstrated that 98% of sera (69/70) collected from Egyptian fruit bats (n = 37) and straw-colored fruit bats (n = 33) had neutralizing antibodies against PRV. Genetic analyses revealed that all 10 genome segments of Nachunsulwe-57 were closely related to a bat-derived Kasama strain found in Uganda. Nachunsulwe-57 showed less efficiency in viral growth and lower pathogenicity in mice than another PRV strain, Miyazaki-Bali/2007, isolated from a patient. CONCLUSIONS: A high proportion of Egyptian fruit bats and straw-colored fruit bats were found to be seropositive to PRV in Zambia. Importantly, a new PRV strain (Nachunsulwe-57) was isolated from an Egyptian fruit bat in Zambia, which had relatively weak pathogenicity in mice. Taken together, our findings provide new epidemiological insights about PRV infection in bats and indicate the first isolation of an PRV strain that may have low pathogenicity to humans.

    DOI: 10.1371/journal.pntd.0009768

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  • Rashid Manzoor, Nao Eguchi, Reiko Yoshida, Hiroichi Ozaki, Tatsunari Kondoh, Kosuke Okuya, Hiroko Miyamoto, Ayato Takada, Mark T. Heise .  A Novel Mechanism Underlying Antiviral Activity of an Influenza Virus M2-Specific Antibody .  Journal of Virology95 ( 1 )   2020.12Reviewed International coauthorship International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    Protective immunity against influenza A viruses (IAVs) generally depends on antibodies to the major envelope glycoprotein, hemagglutinin (HA), whose antigenicity is distinctive among IAV subtypes. On the other hand, the matrix 2 (M2) protein is antigenically highly conserved and has been studied as an attractive vaccine antigen to confer cross-protective immunity against multiple subtypes of IAVs. However, antiviral mechanisms of M2-specific antibodies are not fully understood. Here, we report the molecular basis of antiviral activity of an M2-specific monoclonal antibody (MAb), rM2ss23. We first found that rM2ss23 inhibited A/Aichi/2/1968 (H3N2) (Aichi) but not A/PR/8/1934 (H1N1) (PR8) replication. rM2ss23 altered the cell surface distribution of M2, likely by cross-linking the molecules, and interfered with the colocalization of HA and M2, resulting in reduced budding of progeny viruses. However, these effects were not observed for another strain, PR8, despite the binding capacity of rM2ss23 to PR8 M2. Interestingly, HA was also involved in the resistance of PR8 to rM2ss23. We also found that two amino acid residues at positions 54 and 57 in the M2 cytoplasmic tail were critical for the insensitivity of PR8 to rM2ss2. These findings suggest that the disruption of the M2-HA colocalization on infected cells and subsequent reduction of virus budding is one of the principal mechanisms of antiviral activity of M2-specific antibodies and that anti-M2 antibody-sensitive and -resistant IAVs have different properties in the interaction between M2 and HA.IMPORTANCE Although the IAV HA is the major target of neutralizing antibodies, most of the antibodies are HA subtype specific, restricting the potential of HA-based vaccines. On the contrary, the IAV M2 protein has been studied as a vaccine antigen to confer cross-protective immunity against IAVs with multiple HA subtypes, since M2 is antigenically conserved. Although a number of studies highlight the protective role of anti-HA neutralizing and nonneutralizing antibodies, precise information on the molecular mechanism of action of M2-specific antibodies is still obscure. In this study, we found that an anti-M2 antibody interfered with the HA-M2 association, which is important for efficient budding of progeny virus particles from infected cells. The antiviral activity was IAV strain dependent despite the similar binding capacity of the antibody to M2, and, interestingly, HA was involved in susceptibility to the antibody. Our data provide a novel mechanism underlying antiviral activity of M2-specific antibodies.

    DOI: 10.1128/JVI.01277-20

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  • A biaryl sulfonamide derivative as a novel inhibitor of filovirus infection. .  Antiviral research183   104932 - 104932   2020.11Reviewed International coauthorship International journal

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    Ebolaviruses and marburgviruses, members of the family Filoviridae, are known to cause fatal diseases often associated with hemorrhagic fever. Recent outbreaks of Ebola virus disease in West African countries and the Democratic Republic of the Congo have made clear the urgent need for the development of therapeutics and vaccines against filoviruses. Using replication-incompetent vesicular stomatitis virus (VSV) pseudotyped with the Ebola virus (EBOV) envelope glycoprotein (GP), we screened a chemical compound library to obtain new drug candidates that inhibit filoviral entry into target cells. We discovered a biaryl sulfonamide derivative that suppressed in vitro infection mediated by GPs derived from all known human-pathogenic filoviruses. To determine the inhibitory mechanism of the compound, we monitored each entry step (attachment, internalization, and membrane fusion) using lipophilic tracer-labeled ebolavirus-like particles and found that the compound efficiently blocked fusion between the viral envelope and the endosomal membrane during cellular entry. However, the compound did not block the interaction of GP with the Niemann-Pick C1 protein, which is believed to be the receptor of filoviruses. Using replication-competent VSVs pseudotyped with EBOV GP, we selected escape mutants and identified two EBOV GP amino acid residues (positions 47 and 66) important for the interaction with this compound. Interestingly, these amino acid residues were located at the base region of the GP trimer, suggesting that the compound might interfere with the GP conformational change required for membrane fusion. These results suggest that this biaryl sulfonamide derivative is a novel fusion inhibitor and a possible drug candidate for the development of a pan-filovirus therapeutic.

    DOI: 10.1016/j.antiviral.2020.104932

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  • Hayato Harima, Michihito Sasaki, Masahiro Kajihara, Gabriel Gonzalez, Edgar Simulundu, Eugene C. Bwalya, Yongjin Qiu, Kosuke Okuya, Mao Isono, Yasuko Orba, Ayato Takada, Bernard M. Hang’ombe, Aaron S. Mweene, Hirofumi Sawa .  Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia .  Journal of General Virology101 ( 10 ) 1027 - 1036   2020.10Reviewed International coauthorship International journal

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    Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.

    DOI: 10.1099/jgv.0.001476

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  • Takeshi Saito, Junki Maruyama, Noriyo Nagata, Mao Isono, Kosuke Okuya, Yoshihiro Takadate, Yurie Kida, Hiroko Miyamoto, Akina Mori-Kajihara, Takanari Hattori, Wakako Furuyama, Shinya Ogawa, Shigeru Iida, Ayato Takada .  A Surrogate Animal Model for Screening of Ebola and Marburg Glycoprotein-Targeting Drugs Using Pseudotyped Vesicular Stomatitis Viruses .  Viruses12 ( 9 )   2020.8Reviewed International coauthorship International journal

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    © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Filoviruses, including Ebola virus (EBOV) and Marburg virus (MARV), cause severe hemorrhagic fever in humans and nonhuman primates with high mortality rates. There is no approved therapy against these deadly viruses. Antiviral drug development has been hampered by the requirement of a biosafety level (BSL)-4 facility to handle infectious EBOV and MARV because of their high pathogenicity to humans. In this study, we aimed to establish a surrogate animal model that can be used for anti-EBOV and -MARV drug screening under BSL-2 conditions by focusing on the replication-competent recombinant vesicular stomatitis virus (rVSV) pseudotyped with the envelope glycoprotein (GP) of EBOV (rVSV/EBOV) and MARV (rVSV/MARV), which has been investigated as vaccine candidates and thus widely used in BSL-2 laboratories. We first inoculated mice, rats, and hamsters intraperitoneally with rVSV/EBOV and found that only hamsters showed disease signs and succumbed within 4 days post-infection. Infection with rVSV/MARV also caused lethal infection in hamsters. Both rVSV/EBOV and rVSV/MARV were detected at high titers in multiple organs including the liver, spleen, kidney, and lungs of infected hamsters, indicating acute and systemic infection resulting in fatal outcomes. Therapeutic effects of passive immunization with an anti-EBOV neutralizing antibody were specifically observed in rVSV/EBOV-infected hamsters. Thus, this animal model is expected to be a useful tool to facilitate in vivo screening of anti-filovirus drugs targeting the GP molecule.

    DOI: 10.3390/v12090923

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  • Ulaankhuu A, Bazarragchaa E, Okamatsu M, Hiono T, Bodisaikhan K, Amartuvshin T, Tserenjav J, Urangoo T, Buyantogtokh K, Matsuno K, Hattori T, Kondoh T, Sato M, Takadate Y, Torii S, Isono M, Okuya K, Saito T, Kasajima N, Kida Y, Maruyama J, Igarashi M, Takada A, Kida H, Batchuluun D, Sakoda Y .  Genetic and antigenic characterization of H5 and H7 avian influenza viruses isolated from migratory waterfowl in Mongolia from 2017 to 2019. .  Virus genes56 ( 4 ) 472 - 479   2020.8Reviewed International coauthorship International journal

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    The circulation of highly pathogenic avian influenza viruses (HPAIVs) of various subtypes (e.g., H5N1, H5N6, H5N8, and H7N9) in poultry remains a global concern for animal and public health. Migratory waterfowls play important roles in the transmission of these viruses across countries. To monitor virus spread by wild birds, active surveillance for avian influenza in migratory waterfowl was conducted in Mongolia from 2015 to 2019. In total, 5000 fecal samples were collected from lakesides in central Mongolia, and 167 influenza A viruses were isolated. Two H5N3, four H7N3, and two H7N7 viruses were characterized in this study. The amino acid sequence at hemagglutinin (HA) cleavage site of those isolates suggested low pathogenicity in chickens. Phylogenetic analysis revealed that all H5 and H7 viruses were closely related to recent H5 and H7 low pathogenic avian influenza viruses (LPAIVs) isolated from wild birds in Asia and Europe. Antigenicity of H7Nx was similar to those of typical non-pathogenic avian influenza viruses (AIVs). While HPAIVs or A/Anhui/1/2013 (H7N9)-related LPAIVs were not detected in migratory waterfowl in Mongolia, sporadic introductions of AIVs including H5 and H7 viruses into Mongolia through the wild bird migration were identified. Thus, continued monitoring of H5 and H7 AIVs in both domestic and wild birds is needed for the early detection of HPAIVs spread into the country.

    DOI: 10.1007/s11262-020-01764-2

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  • Kosuke Okuya, Nao Eguchi, Rashid Manzoor, Reiko Yoshida, Shinji Saito, Tadaki Suzuki, Michihito Sasaki, Takeshi Saito, Yurie Kida, Akina Mori-Kajihara, Hiroko Miyamoto, Osamu Ichii, Masahiro Kajihara, Hideaki Higashi, Ayato Takada .  Comparative Analyses of the Antiviral Activities of IgG and IgA Antibodies to Influenza A Virus M2 Protein .  Viruses12 ( 7 ) 780 - 780   2020.7Reviewed International coauthorship International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:{MDPI} {AG}  

    The influenza A virus (IAV) matrix-2 (M2) protein is an antigenically conserved viral envelope protein that plays an important role in virus budding together with another envelope protein, hemagglutinin (HA). An M2-specific mouse monoclonal IgG antibody, rM2ss23, which binds to the ectodomain of the M2 protein, has been shown to be a non-neutralizing antibody, but inhibits plaque formation of IAV strains. In this study, we generated chimeric rM2ss23 (ch-rM2ss23) IgG and IgA antibodies with the same variable region and compared their antiviral activities. Using gel chromatography, ch-rM2ss23 IgA were divided into three antibody subsets: monomeric IgA (m-IgA), dimeric IgA (d-IgA), and trimeric and tetrameric IgA (t/q-IgA). We found that t/q-IgA had a significantly higher capacity to reduce the plaque size of IAVs than IgG and m-IgA, most likely due to the decreased number of progeny virus particles produced from infected cells. Interestingly, HA-M2 colocalization was remarkably reduced on the infected cell surface in the presence of ch-rM2ss23 antibodies. These results indicate that anti-M2 polymeric IgA restricts IAV budding more efficiently than IgG and suggest a role of anti-M2 IgA in cross-protective immunity to IAVs.

    DOI: 10.3390/v12070780

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  • Kosuke Okuya, Reiko Yoshida, Rashid Manzoor, Shinji Saito, Tadaki Suzuki, Michihito Sasaki, Takeshi Saito, Yurie Kida, Akina Mori-Kajihara, Tatsunari Kondoh, Masahiro Sato, Masahiro Kajihara, Hiroko Miyamoto, Osamu Ichii, Hideaki Higashi, Ayato Takada, Colin R. Parrish .  Potential Role of Nonneutralizing IgA Antibodies in Cross-Protective Immunity against Influenza A Viruses of Multiple Hemagglutinin Subtypes .  Journal of Virology94 ( 12 )   2020.6Reviewed International coauthorship International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    <jats:p>IgA antibodies on mucosal surfaces are known to play an important role in protection from influenza A virus (IAV) infection and are believed to be more potent than IgG for cross-protective immunity against IAVs of multiple hemagglutinin (HA) subtypes. However, in general, neutralizing antibodies specific to HA are principally HA subtype-specific. Here we focus on non-neutralizing but broadly cross-reactive HA-specific IgA antibodies. Recombinant IgG, monomeric IgA (mIgA), and polymeric secretory IgA (pSIgA) antibodies were generated based on the sequence of a mouse anti-HA monoclonal antibody (MAb) 5A5 that had no neutralizing activity but showed broad binding capacity to multiple HA subtypes. While confirming that there was no neutralizing activity of the recombinant MAbs against IAV strains A/Puerto Rico/8/1934 (H1N1), A/Adachi/2/1957 (H2N2), A/Hong Kong/483/1997 (H5N1), A/shearwater/South Australia/1/1972 (H6N5), A/duck/England/1/1956 (H11N6), and A/duck/Alberta/60/1976 (H12N5), we found that pSIgA, but not mIgA and IgG, significantly reduced budding and release of most of the viruses from infected cells. Electron microscopy demonstrated that pSIgA deposited newly produced virus particles on the surfaces of infected cells, most likely due to tethering of virus particles. Furthermore, we found that pSIgA showed significantly higher activity to reduce plaque sizes of the viruses than IgG and mIgA. These results suggest that non-neutralizing pSIgA reactive to multiple HA subtypes may play a role in inter-subtype cross-protective immunity against IAVs.</jats:p>
    <jats:p><jats:bold>Importance</jats:bold> Mucosal immunity represented by pSIgA plays important roles in protection from IAV infection. Furthermore, IAV HA-specific pSIgA antibodies are thought to contribute to cross-protective immunity against multiple IAV subtypes. However, the mechanisms by which pSIgA exerts such versatile antiviral activity are not fully understood. In this study, we generated broadly cross-reactive recombinant IgG and pSIgA having the same antigen-recognition site and compared their antiviral activities <jats:italic>in vitro</jats:italic>. These recombinant antibodies did not show “classical” neutralizing activity, whereas pSIgA, but not IgG, significantly inhibited the production of progeny virus particles from infected cells. Plaque formation was also significantly reduced by pSIgA, but not IgG. These effects were seen in infection with IAVs of several different HA subtypes. Based on our findings, we propose an antibody-mediated host defense mechanism by which mucosal immunity may contribute to broad cross-protection from IAVs of multiple HA subtypes, including viruses with pandemic potential.</jats:p>

    DOI: 10.1128/JVI.00408-20

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  • Genetic and Biological Diversity of Porcine Sapeloviruses Prevailing in Zambia. .  Viruses12 ( 2 )   2020.2Reviewed International coauthorship International journal

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    Porcine sapelovirus (PSV) has been detected worldwide in pig populations. Although PSV causes various symptoms such as encephalomyelitis, diarrhea, and pneumonia in pigs, the economic impact of PSV infection remains to be determined. However, information on the distribution and genetic diversity of PSV is quite limited, particularly in Africa. In this study, we investigated the prevalence of PSV infection in Zambia and characterized the isolated PSVs genetically and biologically. We screened 147 fecal samples collected in 2018 and found that the prevalences of PSV infection in suckling pigs and fattening pigs were high (36.2% and 94.0%, respectively). Phylogenetic analyses revealed that the Zambian PSVs were divided into three different lineages (Lineages 1-3) in the clade consisting of Chinese strains. The Zambian PSVs belonging to Lineages 2 and 3 replicated more efficiently than those belonging to Lineage 1 in Vero E6 and BHK cells. Bioinformatic analyses revealed that genetic recombination events had occurred and the recombination breakpoints were located in the L and 2A genes. Our results indicated that at least two biologically distinct PSVs could be circulating in the Zambian pig population and that genetic recombination played a role in the evolution of PSVs.

    DOI: 10.3390/v12020180

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  • Marburgvirus in Egyptian Fruit Bats, Zambia. .  Emerging infectious diseases25 ( 8 ) 1577 - 1580   2019.8Reviewed International coauthorship International journal

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    We detected Marburg virus genome in Egyptian fruit bats (Rousettus aegyptiacus) captured in Zambia in September 2018. The virus was closely related phylogenetically to the viruses that previously caused Marburg outbreaks in the Democratic Republic of the Congo. This finding demonstrates that Zambia is at risk for Marburg virus disease.

    DOI: 10.3201/eid2508.190268

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  • Ozawa M, Matsuu A, Khalil AMA, Nishi N, Tokorozaki K, Masatani T, Horie M, Okuya K, Ueno K, Kuwahara M, Toda S .  Phylogenetic variations of highly pathogenic H5N6 avian influenza viruses isolated from wild birds in the Izumi plain, Japan during the 2016/17 winter season. .  Transboundary and emerging diseases66 ( 2 ) 797 - 806   2019.3Reviewed International coauthorship International journal

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    During the 2016-2017 winter season, we isolated 33 highly pathogenic avian influenza viruses (HPAIVs) of H5N6 subtype and three low pathogenic avian influenza viruses (LPAIVs) from debilitated or dead wild birds, duck faeces, and environmental water samples collected in the Izumi plain, an overwintering site for migratory birds in Japan. Genetic analyses of the H5N6 HPAIV isolates revealed previously unreported phylogenetic variations in the PB2, PB1, PA, and NS gene segments and allowed us to propose two novel genotypes for the contemporary H5N6 HPAIVs. In addition, analysis of the four gene segments identified close phylogenetic relationships between our three LPAIV isolates and the contemporary H5N6 HPAIV isolates. Our results implied the co-circulation and co-evolution of HPAIVs and LPAIVs within the same wild bird populations, thereby highlighting the importance of avian influenza surveillance targeting not only for HPAIVs but also for LPAIVs.

    DOI: 10.1111/tbed.13087

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  • Okuya K, Matsuu A, Kawabata T, Koike F, Ito M, Furuya T, Taneno A, Akimoto S, Deguchi E, Ozawa M .  Distribution of gene segments of the pandemic A(H1N1) 2009 virus lineage in pig populations. .  Transboundary and emerging diseases65 ( 6 ) 1502 - 1513   2018.12Reviewed International coauthorship International journal

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    DOI: 10.1111/tbed.12887

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  • Nakagawa H, Okuya K, Kawabata T, Matsuu A, Takase K, Kuwahara M, Toda S, Ozawa M .  Genetic characterization of low-pathogenic avian influenza viruses isolated on the Izumi plain in Japan: possible association of dynamic movements of wild birds with AIV evolution. .  Archives of virology163 ( 4 ) 911 - 923   2018.4Reviewed International coauthorship International journal

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    The Izumi plain in Kagoshima Prefecture, Japan, is an overwintering site of endangered cranes (hooded cranes and white-naped cranes) and of many other migratory birds (including wild ducks) that are considered carriers of avian influenza viruses (AIVs). To assess the risks of a highly pathogenic avian influenza outbreak in the crane populations, we tested various environmental samples for AIVs in this area. In the 2014-2015 winter season, we isolated one AIV of the H6N2 subtype from the cranes' roost water and two AIVs of the H11N9 subtype from a crane fecal sample and a cloacal swab of a dead spot-billed duck. Genetic analysis of these AIV isolates indicated that our H6N2 isolate is genetically close to AIVs isolated from wild birds in Southeast Asian countries, except that the PB1 and NS genes belong to the North American virus lineage. All genes of the two H11N9 isolates are related to AIVs belonging to the Eurasian virus lineage. Notably, in our phylogenetic trees, H11 HA and N9 NA genes showing high sequence similarity to the corresponding genes of isolates from wild birds in South Africa and Spain, respectively, did not cluster in the major groups with recent wild-bird isolates from East Asia. These results suggest that AIVs with viral gene segments derived from various locations and bird species have been brought to the Izumi plain. These findings imply a possible association of dynamic movements of wild birds with AIV evolution.

    DOI: 10.1007/s00705-017-3698-1

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  • Kosuke Okuya, Norihiro Kanazawa, Takehiro Kanda, Masakazu Kuwahara, Aya Matsuu, Masayuki Horie, Tatsunori Masatani, Shigehisa Toda, Makoto Ozawa .  Genetic characterization of an avian H4N6 influenza virus isolated from the Izumi plain, Japan. .  Microbiology and immunology61 ( 11 ) 513 - 518   2017.11Reviewed International coauthorship International journal

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    An influenza A virus of H4N6 subtype was isolated from the Izumi plain, Japan, in 2013. Genetic analyses revealed that two viral genes (M and NS gene segments) of this isolate were genetically distinct from those of the H4N6 virus isolated from the same place in 2012. Furthermore, three viral genes (PB2, PB1 and M gene segments) of this isolate share high similarity with those of the North American isolates of 2014. These results suggest a high frequency of genetic reassortment of avian influenza viruses in Asian waterfowl and intercontinental movements of avian influenza viruses via migratory waterfowl.

    DOI: 10.1111/1348-0421.12545

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  • Masatani T, Ozawa M, Yamada K, Ito N, Horie M, Matsuu A, Okuya K, Tsukiyama-Kohara K, Sugiyama M, Nishizono A .  Contribution of the interaction between the rabies virus P protein and I-kappa B kinase ϵ to the inhibition of type I IFN induction signalling. .  The Journal of general virology97 ( 2 ) 316 - 326   2016.2Reviewed International coauthorship International journal

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    The P protein of rabies virus (RABV) is known to interfere with the phosphorylation of the host IFN regulatory factor 3 (IRF-3) and to consequently inhibit type I IFN induction. Previous studies, however, have only tested P proteins from laboratory-adapted fixed virus strains, and to the best of our knowledge there is no report about the effect of P proteins from street RABV strains or other lyssaviruses on the IRF-3-mediated type I IFN induction system. In this study, we evaluated the inhibitory effect of P proteins from several RABV strains, including fixed and street virus strains and other lyssaviruses (Lagos bat, Mokola and Duvenhage viruses), on IRF-3 signalling. All P proteins tested inhibited retinoic acid-inducible gene-1 (RIG-I)- and TANK binding kinase 1 (TBK1)-mediated IRF-3-dependent IFN-β promoter activities. On the other hand, the P proteins from the RABV street strains 1088 and HCM-9, but not from fixed strains Nishigahara (Ni) and CVS-11 and other lyssaviruses tested, significantly inhibited I-kappa B kinase ϵ (IKKϵ)-inducible IRF-3-dependent IFN-β promoter activity. Importantly, we revealed that the P proteins from the 1088 and HCM-9 strains, but not from the remaining viruses, interacted with IKKϵ. By using expression plasmids encoding chimeric P proteins from the 1088 strain and Ni strain, we found that the C-terminal region of the P protein is important for the interaction with IKKϵ. These findings suggest that the P protein of RABV street strains may contribute to efficient evasion of host innate immunity.

    DOI: 10.1099/jgv.0.000362

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  • Ozawa M, Kawabata T, Okuya K, Nagano K, Kanda T, Kanazawa N, Tsukiyama-Kohara K, Taneno A, Deguchi E .  Full genome sequences of torque teno sus virus strains that coinfected a pig with postweaning multisystemic wasting syndrome in Japan: implications for genetic diversity. .  Archives of virology160 ( 12 ) 3067 - 74   2015.12Reviewed International coauthorship International journal

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    We determined the complete genome sequences of torque teno sus viruses (TTSuVs) detected in pigs with postweaning multisystemic wasting syndrome (PMWS) and in healthy pigs in Japan. Unexpectedly, we found coinfection of a PMWS-affected pig in Japan with one strain of TTSuV1, five strains of TTSuV2, and one strain of PCV2. Full-genome sequencing of each of these strains, followed by phylogenetic analysis, revealed broad genetic diversity in the TTSuV2 strains infecting the PMWS-affected pig. These results suggest that the geographical bias in the available genetic information about TTSuVs has a limited impact on the evaluation of their genetic diversity.

    DOI: 10.1007/s00705-015-2593-x

    PubMed

  • Okuya K, Kawabata T, Nagano K, Tsukiyama-Kohara K, Kusumoto I, Takase K, Ozawa M .  Isolation and characterization of influenza A viruses from environmental water at an overwintering site of migratory birds in Japan. .  Archives of virology160 ( 12 ) 3037 - 52   2015.12Reviewed International coauthorship International journal

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    The Izumi plain in Kagoshima prefecture, Japan, is an overwintering site of more than 10,000 cranes. The wet paddy areas are artificially created to provide roosting sites for the cranes every winter. Since wild ducks, known to be a natural reservoir of influenza A viruses, also overwinter in this area, the cranes' roost water likely serves as a source of influenza A virus infection. To assess this potential risk, we collected 126 water samples from the cranes' roost in the 2012/2013 winter season for virus isolation. We isolated six influenza viruses of three subtypes (H3N8, H4N6, and H4N8) from the water samples collected in the months of November and December. Genetic analysis of our isolates indicated that these viruses were genetically similar to the low-pathogenic avian influenza viruses circulating among Eurasian waterfowl. These findings suggest the possibility of the cranes becoming infected with the avian influenza viruses that are present in their roost water.

    DOI: 10.1007/s00705-015-2610-0

    PubMed

  • Ozawa M, Matsuu A, Yonezawa K, Igarashi M, Okuya K, Kawabata T, Ito K, Tsukiyama-Kohara K, Taneno A, Deguchi E .  Efficient isolation of Swine influenza viruses by age-targeted specimen collection. .  Journal of clinical microbiology53 ( 4 ) 1331 - 8   2015.4Reviewed International coauthorship International journal

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    The control of swine influenza virus (SIV) infection is paramount for increasing the productivity of pig farming and minimizing the threat of pandemic outbreaks. Thus, SIV surveillance should be conducted by region and on a regular basis. Here, we established a microneutralization assay specific for SIV seroprevalence surveillance by using reporter gene-expressing recombinant influenza viruses. Growth-based SIV seroprevalence revealed that most sows and piglets were positive for neutralizing antibodies against influenza viruses. In contrast, the 90-day-old growing pigs exhibited limited neutralizing activity in their sera, suggesting that this particular age of population is most susceptible to SIV infection and thus is an ideal age group for SIV isolation. From nasal swab specimens of healthy pigs in this age population, we were able to isolate SIVs at a higher incidence (5.3%) than those of previous reports. Nucleotide sequencing and phylogenetic analysis of the hemagglutinin (HA) genes revealed that the isolated SIVs have circulated and evolved in pigs but not have been recently introduced from humans, implying that a large number of SIV lineages may remain "undiscovered" in the global porcine populations. We propose that the 90-day-old growing pig-targeted nasal swab collection presented in this study facilitates global SIV surveillance and contributes to the detection and control of SIV infection.

    DOI: 10.1128/jcm.02941-14

    PubMed

  • Ozawa M, Matsuu A, Tokorozaki K, Horie M, Masatani T, Nakagawa H, Okuya K, Kawabata T, Toda S .  Genetic diversity of highly pathogenic H5N8 avian influenza viruses at a single overwintering site of migratory birds in Japan, 2014/15. .  Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin20 ( 20 )   2015Reviewed International coauthorship International journal

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    We isolated eight highly pathogenic H5N8 avian influenza viruses (H5N8 HPAIVs) in the 2014/15 winter season at an overwintering site of migratory birds in Japan. Genetic analyses revealed that these isolates were divided into three groups, indicating the co-circulation of three genetic groups of H5N8 HPAIV among these migratory birds. These results also imply the possibility of global redistribution of the H5N8 HPAIVs via the migration of these birds next winter.

    PubMed

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Books

  • 「鶏の研究」2022年1月号

    ( Role: Contributor ,  家禽農場における高病原性鳥インフルエンザの制御)

    鶏の研究・木香書房   2022.1 

  • 「鶏の研究」2021年12月号

    ( Role: Contributor ,  野鳥における鳥インフルエンザサーベイランスの重要性)

    鶏の研究・木香書房  2021.12 

MISC

  • 野鳥渡来地の環境水から紐解く鳥インフルエンザウイルスの流行動態

    KHALIL Ahmed Magdy, 奥谷公亮, 奥谷公亮, 藤本佳万, 藤本佳万, 児島一州, 江崎真南, 李京河, 西奈津子, 正谷達謄, 松井勉, 小澤真, 小澤真, 小澤真

    日本獣医学会学術集会講演要旨集   164th (CD-ROM)   2021

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  • ザンビアのブタにおける消化管ウイルスの疫学調査

    播磨勇人, 梶原将大, 佐々木道仁, SIMULUNDU Edgar, BWALYA Eugene, 邱永晋, 奥谷公亮, 大場靖子, HANG’OMBE Bernard M, MWEENE Aaron S, 高田礼人, 澤洋文

    日本獣医学会学術集会講演要旨集   164th (CD-ROM)   2021

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  • Single nucleotide polymorphisms in human angiotensin-converting enzyme 2 receptor and cell susceptibility to SARS-CoV-2

    HATTORI Takanari, SAITO Takeshi, TAKADATE Yoshihiro, OKUYA Kosuke, KASAJIMA Nodoka, KAJIHARA Akina, MIYAMOTO Hiroko, KAJIHARA Masahiro, TAKADA Ayato

    日本ウイルス学会学術集会プログラム・予稿集(Web)   68th   2021

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  • A型インフルエンザウイルスに対する交差感染防御免疫への非中和IgA抗体の役割

    奥谷公亮, 吉田玲子, マンズール ラシッド, 齊藤慎二, 鈴木忠樹, 市居修, 東秀明, 高田礼人

    日本ウイルス学会学術集会プログラム・予稿集(Web)   67th   2019

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  • ザンビアのエジプトルーセットオオコウモリから検出されたマールブルグウイルスの遺伝学的解析

    梶原将大, CHANGULA Katendi, HANG’OMBE Bernard, 播磨勇人, 宮本洋子, 衛藤芳樹, 奥谷公亮, 磯野真央, 吉田玲子, 邱永晋, 森(梶原)亜紀奈, 大場靖子, 澤洋文, 澤洋文, 小川寛仁, SIMULUNDU Edgar, SQUARRE David, MUKONKA Victor, MWEENE Aaron, 高田礼人, 高田礼人

    日本ウイルス学会学術集会プログラム・予稿集(Web)   67th   2019

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  • エボラウイルスの細胞侵入阻害薬の探索

    磯野真央, 古山若呼, 黒田誠, 近藤達成, 五十嵐学, 梶原将大, 吉田玲子, マンズール ラシッド, 奥谷公亮, 宮本洋子, フェルドマン ハインツ, マルジ アンドレア, 堺谷政弘, 高田礼人

    日本ウイルス学会学術集会プログラム・予稿集(Web)   67th   2019

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  • 豚インフルエンザウイルスの遺伝的特性の解明

    奥谷公亮, 松鵜彩, 小澤真, 小澤真

    日本産業動物獣医学会(九州)・日本小動物獣医学会(九州)・日本獣医公衆衛生学会(九州)   2016   2016

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  • 2014‐15年冬季に鹿児島県出水平野で分離されたH5N8亜型高病原性鳥インフルエンザウイルスの解析と感染野鳥における特徴

    松鵜彩, 所崎香織, 寸田祐嗣, 森田剛仁, 川口博明, 堀江真行, 正谷達謄, 中川寛子, 奥谷公亮, 川畑淑子, 戸田重久, 小原恭子, 小原恭子, 小澤真, 小澤真

    日本獣医学会学術集会講演要旨集   158th   329 - 329   2015.8

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    Language:Japanese   Publisher:(公社)日本獣医学会  

    J-GLOBAL

  • ツルのインフルエンザウイルスに対する感受性の検討

    奥谷公亮, 川畑淑子, 永野希織, 小原恭子, 小原恭子, 楠元勇, 高瀬公三, 高瀬公三, 小澤真, 小澤真

    日本獣医学会学術集会講演要旨集   157th   2014

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  • 豚トルクテノウイルスの遺伝的多様性

    小澤真, 小澤真, 川畑淑子, 奥谷公亮, 永野希織, 神田雄大, 金澤伯弘, 小原恭子, 小原恭子, 出口栄三郎, 出口栄三郎

    日本獣医学会学術集会講演要旨集   157th   2014

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  • 豚インフルエンザウイルスの効率的なサーベイランス方法の検討

    小澤真, 小澤真, 松鵜彩, 米澤弘毅, 五十嵐学, 奥谷公亮, 川畑淑子, 伊藤公人, 小原恭子, 小原恭子, 種子野章, 出口栄三郎

    日本ウイルス学会学術集会プログラム・抄録集   62nd   2014

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Presentations

  • 齋藤健, 奥谷公亮, 服部貴成, 梶原将大, 高田礼人   Molecular mechanisms underlying the cellular entry and host range restriction of Lujo virus  

    第68回日本ウイルス学会学術集会 

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    Event date: 2021.11

    Language:English   Presentation type:Oral presentation (general)  

  • 服部貴成, 斎藤健, 高館佳弘, 奥谷公亮, 笠島和, 梶原亜紀奈, 宮本洋子, 梶原将大, 高田礼人   Single nucleotide polymorphisms in human angiotensin-converting enzyme 2 receptor and cell susceptibility to SARS-CoV-2  

    第68回日本ウイルス学会学術集会 

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    Event date: 2021.11

  • 児島一州, 尾野本浩司, 内藤清惟, 奧谷公亮, 岡島美鈴, 伊藤直人, 杉山誠, 小澤真, 米山光俊, 藤田尚志, 正谷達謄   Stress granule formation mechanism related to amino acid substitution at position 95 in rabies virus matrix protein  

    第68回日本ウイルス学会学術集会  2021.11 

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    Event date: 2021.11

  • 児島一州、尾野本浩司、内藤清惟、岡島美鈴、奧谷公亮、伊藤直人、杉山誠、小澤真、米山光俊、藤田尚志、正谷達謄   狂犬病ウイルスM蛋白質95位のアミノ酸はストレス顆粒形成に関与する  

    第164回日本獣医学会学術集会 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 播磨 勇人、梶原 将大、佐々木 道仁、Simulundu Edgar、Bwalya Eugene、邱 永晋、奥谷 公亮、大場 靖子、Hang'ombe Bernard M、Mweene Aaron S、高田 礼人、澤 洋文   ザンビアのブタにおける消化管ウイルスの疫学調査  

    第164回日本獣医学会学術集会 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • Ahmed Magdy Khali、奥谷 公亮、藤本 佳万、児島 一州、江崎 真南、李 京河、西 奈津子、正谷 達謄、松井 勉、小澤 真   野鳥渡来地の環境水から紐解く鳥インフルエンザウイルスの流行動態  

    第164回日本獣医学会学術集会 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 奥谷公亮、吉田玲子、マンズールラシッド、齊藤慎二、鈴木忠樹、市居修、東秀明、高田礼人   A型インフルエンザウイルスの亜型間交差感染防御における非中和IgA抗体の役割  

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    Event date: 2020.1

    Language:Japanese   Presentation type:Oral presentation (general)  

  • A potential role of non-neutralizing IgA antibody for cross-protective immunity against influenza A viruses  

    第67回日本ウイルス学会学術集会 

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    Event date: 2019.10

    Language:English   Presentation type:Oral presentation (general)  

  • Okuya K, Eguchi N, Manzoor R, Higashi H, Suzuki T, Yoshida R, Takada A   Comparative analyses of the antiviral activity between M2-specific IgG and IgA antibodies against influenza A viruses   International conference

    the 6th Sapporo Symposium for One Health 

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    Event date: 2018.9

    Language:English   Presentation type:Poster presentation  

  • 8. Okuya K, Eguchi N, Manzoor R, Higashi H, Suzuki T, Yoshida R, Takada A   Comparative analyses of the antiviral activity between M2-specific IgG and IgA antibodies against influenza A viruses  

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    Event date: 2018.7

    Language:English   Presentation type:Oral presentation (general)  

  • 奥谷公亮、江口直、マンズール ラシッド、東秀明、鈴木忠樹、吉田玲子、高田礼人   A型インフルエンザウイルスにおける抗M2タンパク質IgGおよびIgA 抗体による抗ウイルス活性の比較解析  

    第15回日本ウイルス学キャンプin湯河原 

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    Event date: 2018.5

    Language:Japanese   Presentation type:Oral presentation (general)  

  • Makoto Ozawa,Aya Matsuu,Natsuko Nishi,Kaori Tokorozaki,Tatsunori Masatani, Masayuki Horie,Kosuke Okuya,Kosei Ueno,Shigehisa Toda   Genetic composition of highly pathogenic H5N6 avian influenza viruses isolated at the Izumi plain, Kagoshima, Japan  

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    Event date: 2017.10

    Language:English   Presentation type:Oral presentation (general)  

  • 奥谷公亮、 松鵜彩、小澤真   豚インフルエンザウイルスの遺伝的特性の解明  

    平成28年度獣医学術九州地区学会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 奥谷公亮、 八田正人、小澤真、Gabriele Neumann、河岡義裕   A型インフルエンザウイルスにおけるH5亜型の抗原性決定部位の探索  

    日本生化学学会九州支部会 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 松鵜 彩、所崎 香識、寸田 祐嗣、森田 剛仁、川口 博明、堀江 真行、正谷 達謄、中川 寛子、奥谷 公亮、川畑 淑子、戸田 重久、小原 恭子、小澤 真   2014-15年冬季に鹿児島県出水平野で分離されたH5N8亜型高病原性鳥インフルエンザウイルスの解析と感染野鳥における特徴  

    第158回日本獣医学会学術集会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 小澤 真、松鵜 彩、米澤 弘毅、五十嵐 学、奥谷 公亮、川畑 淑子、伊藤 公人、小原 恭子、種子野 章、出口 栄三郎   豚インフルエンザウイルスの効率的なサーベイランス方法の検討  

    第62回日本ウイルス学会学術集会 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

  • Development of Tupaia belangeri for HBV persistent infection  

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    Event date: 2014.11

    Language:English   Presentation type:Oral presentation (general)  

  • 奥谷公亮、川畑淑子、永野希織、小原恭子、楠元勇、高瀬公三、小澤真   ツルのインフルエンザウイルスに対する感受性の検討  

    第157回日本獣医学会学術集会 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 小澤 真、川畑 淑子、奥谷 公亮、永野 希織、神田 雄大、金澤 伯弘、小原 恭子、出口 栄三郎   豚トルクテノウイルスの遺伝的多様性  

    第157回日本獣医学会学術集会 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 池 海英、Ezzikouri, S.、真田 崇弘、永野 希織、山口 千穂、神田 雄大、金澤 伯弘、奥谷 公亮、上野 晃聖、中川 寛子、Nkogue, C.N.、小澤 真、小原 道法、小原 恭子   ツパイを用いたHCV感染・発症モデル系並びに治療評価系の開発  

    第51回日本ウイルス学会九州支部総会 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 小澤 真、奥谷 公亮、川畑 淑子、永野 希織、 楠元 勇、高瀬 公三、小原 恭子   ツル越冬地の水からのインフルエンザウイルスの分離  

    第61回日本ウイルス学会学術集会 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 奥谷公亮、川畑淑子、永野希織、小原恭子、楠元勇、高瀬公三、小澤真   ツルのねぐらにおけるインフルエンザウイルスの分離  

    第156回日本獣医学会学術集会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

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Research Projects

  • 養豚場における豚インフルエンザウイルスの感染流行動態の解明

    Grant number:22K14995  2022.4 - 2024.3

    日本学術振興会  科学研究費助成事業(学術研究助成基金助成金)  若手研究

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    Authorship:Principal investigator  Grant type:Competitive

  • 日本国内のコウモリにおける新規ウイルスの性状解析

    2022.4 - 2023.3

    北海道大学人獣共通感染症国際共同研究所   共同研究拠点事業 

  • 豚サーコウイルス3型のウイルス学的性状の解明

    2022.3 - 2024.3

    公益財団法人清川秋夫育英奨学財団  研究助成事業 

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    Authorship:Principal investigator 

  • 鳥インフルエンザウイルスの迅速な塩基配列解析法の開発 International coauthorship

    Grant number:21K20610  2021.10 - 2023.3

    日本学術振興会  科学研究費助成事業(学術研究助成基金助成金)  研究活動スタート支援

    奥谷 公亮

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    Authorship:Principal investigator  Grant type:Competitive

  • A型インフルエンザウイルスの亜型間交差感染防御免疫に携わるIgA抗体の機能解析 International coauthorship

    Grant number:18J20917  2018.4 - 2021.3

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    奥谷 公亮

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    Authorship:Principal investigator  Grant type:Competitive

    ウイルス特異的IgA抗体が従来の中和活性とは異なるメカニズム (ウイルス出芽阻害) によって、A型インフルエンザウイルスの亜型間交差感染防御免疫に寄与することがこれまでの研究から示唆されてきた。本研究は、HA亜型間で交差反応性を有するが、中和活性を持たない (非中和) IgA抗体に着目し、その抗ウイルス活性を検証している。
    複数の異なる亜型のHAに結合能を有する非中和抗体の可変領域の遺伝子配列を元に構築した抗体発現プラスミドを用いて、同じ可変領域をもつIgGおよびIgA抗体を作出した。IgA抗体はさらに単量体と多量体に分画した。IgGとIgA抗体との間で、6株の異なるHA亜型 (H1, H2, H5, H6, H11, H12) のA型インフルエンザウイルスに対するウイルス出芽阻害活性を比較した。その結果、多量体IgA抗体を加えた場合に、H2亜型ウイルス以外のウイルス株の出芽量がIgG抗体を加えた場合と比較して有意に減少した。多量体IgA抗体存在下ではウイルス粒子が感染細胞表面に集積している像が電子顕微鏡で確認された。作出した抗体はいずれのウイルス株に対してもNA阻害活性を有さなかったことから、多量体IgA抗体は細胞表面上とウイルス粒子表面上のHAに結合することで、ウイルス粒子の放出を阻害することが示唆された。さらに、ウイルス増殖の指標であるプラークサイズを比較したところ、多量体IgA抗体存在下では、IgG抗体存在下と比較して、H2亜型ウイルス以外のウイルス株のプラークサイズが有意に小さくなった。以上から、非中和多量体IgA抗体はウイルスの放出を阻害することでウイルスの増殖を阻害することが示された。