記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Aquatic Toxicology  

Polycyclic aromatic hydrocarbons (PAHs) are known to induce developmental malformations in fish embryos. However, the interaction between aryl hydrocarbon receptor (AhR) and cytochrome P450 (CYP) in PAH-induced development defects remains unclear. Therefore, we investigated the effects of the CYP1A inhibitor piperonylbutoxide (PBO) and the AhR antagonist CH223191 (CH) on the development of Japanese medaka (Oryzias Latipes) embryos exposed to different PAHs. Japanese medaka embryos were exposed to three conditions: PAH alone, PAH and PBO, and PAH and CH. Microscopic observations were performed to examine the presence of developmental defects. Although neither phenanthrene (Phe) nor fluoranthene (Flu) induced morphological malformations in larvae, benzo(a)anthracene (BaA) exposure induced craniofacial deformities in the larvae. Additionally, BaA and PBO co-exposure significantly increased the rate and severity of malformations. Pyrene (Pyr) exposure induced craniofacial defects, cardiac hypertrophy, pericardial edema, and spinal curvature, which were attenuated by exposure to either CH or PBO. Collectively, these findings suggest that structurally different PAHs exert their toxic effects via distinct mechanisms during fish development.

DOI： 10.1016/j.aquatox.2025.107267

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Chemosphere  

Diphenhydramine (DPH) is an antihistamine drug. It has been frequently detected in the environment, because it is not completely degraded in wastewater treatment plants. Recent studies have shown the adverse effects of DPH exposure to various aquatic organisms; however, its chronic effects on fish have been poorly elucidated. In this study, several pairs of mature Japanese medaka (Oryzias latipes) were exposed to DPH for a long period to determine the effects of DPH exposure on the subsequent generations, number of spawned and fertilized eggs, expression of sex-related genes, feeding behavior, embryo development, hatching rate, malformations among the hatched larvae, and mortality rate. The number of spawned eggs significantly decreased, when the parent fish were continuously exposed to 31.6 μg/L DPH for over 46 days. DPH exposure also altered the feeding behavior of medaka individuals, and increased the larval mortality rate. The effects of DPH exposure to fish may occur to some extent in the actual aquatic environment, although the risk evaluations in the field are limited.

DOI： 10.1016/j.chemosphere.2024.142163

Scopus

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

The relationship of histopathological changes and the infection of Piscine orthoreovirus 2 (PRV-2) was investigated in coho salmon that were suffering from the erythrocytic inclusion body syndrome (EIBS). Immunohistochemical observations revealed abundant σ1 protein of PRV-2 in the spongy layer of the ventricle of the heart, where severe myocarditis was observed. In the spleen, the virus protein was detected in many erythrocytes, some of which were spherical-shaped and apparently dead. The number of erythrocytes was decreased in the spleen compared to the apparently healthy fish. The virus protein was also detected in some erythrocytes in blood vessels. The viral protein was often detected in many macrophages ingesting erythrocytes or dead cell debris in the spleen or in the kidney sinusoids. Large amounts of the viral genomic segment L2 were also detected in these organs by RT-qPCR. Many necrotic foci were found in the liver, although the virus protein was not detected in the hepatocytes. These results suggest that the primary targets of PRV-2 are myocardial cells and erythrocytes and that clinical symptoms such as anaemia or jaundice and histopathological changes such as myocarditis in EIBS-affected coho salmon are caused by PRV-2 infection.

DOI： 10.1111/jfd.13939

PubMed

J-GLOBAL

記述言語：英語   掲載種別：研究論文（学術雑誌）