研究者詳細 - 大岡　唯祐

論文 - 大岡　唯祐

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Fernandes I, Ooka T, Lira D, Martins FH, Orsi H, Jones N, Elias W, Hayashi T, Gomes TA, and Hernandes R. . TccP4: A novel effector identified in the Escherichia albertii strain 1551-2 required for attaching and effacing lesion formation on infected Nck-null cells . Microbiology Spectrum 2025年1月査読国際共著

大岡唯祐、志多田千恵、高橋元秀 . 奄美群島における破傷風菌の分布および株の特性の解析 . 奄美ミッション活動報告書2024 2024年

Mizuno S, Tsukamura Y, Nishio S, Ishida T, Hasegawa D, Kosaka Y, Ooka T, Nishi J, Kasai M. . Catheter-related bloodstream infection caused by Tsukamurella tyrosinosolvens identified by secA1sequencing in an immunocompromised child: a case report. . Annals of Clinical Microbiology and Antimicrobials22 ( 1 ) 97 2023年11月査読

大岡唯祐, 藺牟田直子, 西順一郎, 森田喜紀, 今村真理, 重久朋史, 上野伸広, 相星壮吾, 山本真実, 新川奈緒美, 川村英樹, 馬場洋一, 柿澤広美, 伊藤武, 後藤恭宏, 林哲也 . 2018年のSalmonella enterica serovar Oranienburg菌血症集積事例株と同一クローン由来株による新たな感染事例と各地の食品取り扱い従事者からの同クローンの分離 . 病原微生物検出情報44 127 - 128 2023年8月査読

Koji Ichiki, Tadasuke Ooka, Tetsuhiro Shinkawa, Sakura Inoue, Maiko Hayashida, Daisuke Nakamura, Masaki Akimoto, Makoto Yoshimitsu, Hideki Kawamura, Masatoshi Nakamura, Yuki Obama, Yasuhiro Gotoh, Tetsuya Hayashi, Junichiro Nishi, Kenji Ishitsuka . Genomic and phylogenetic characterization of Elizabethkingia anophelis strains: The first two cases of life-threatening infection in Japan . Journal of infection and chemotherapy29 ( 4 ) 376 - 383 2023年1月査読国際誌

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

OBJECTIVE: Elizabethkingia anophelis causes meningitis, bloodstream infections, and respiratory infections in immunocompromised individuals. We examined two E. anophelis strains isolated from the first life-threatening cases caused by this species in Japan to determine the phylogenetic origin and genomic features of them. METHODS: We performed whole genome-based analysis to clarify the genetic relationship for the two strains (EK0004 and EK0079) and Elizabethkingia sp. strains isolated from worldwide and to characterize the genomic features such as the prevalence of virulence- and antimicrobial resistance (AMR)-related genes. PATIENTS: A 29-year-old man with hepatosplenic T-cell lymphoma and a 52-year-old man with systemic lupus erythematosus developed fatal bacteremia and meningitis due to E. anophelis, respectively. RESULTS: Two strains, EK0004 and EK0079, were genetically different but most closely related to the strains isolated from the largest outbreak in Wisconsin, USA from 2015 to 2016, and the strain isolated from cerebrospinal fluid of a patient in Florida, USA in 1982, respectively. The two strains contained AMR-related genes such as those encoding for an extended-spectrum β-lactamase and multiple metallo-β-lactamases and several virulence-related genes such as capsular polysaccharide synthesis gene clusters. CONCLUSIONS: Although further functional analyses are required to understand the virulence of these clones, these finding suggests that enough caution of E. anophelis infection in immunocompromised patients is required since the number of infections by this species is increasing outside Japan.

DOI： 10.1016/j.jiac.2023.01.005

PubMed

Tadasuke Ooka, Yasuhiro Gotoh, Shigeki Hatanaka, Miyuki Yoshimori, Kazutaka Nishitarumizu, Kanami Kojo, Hiroki Kosakamoto, Koji Sameshima, Yoichi Kuroki, Naomi Chibana, Yuriko Doi, Shuji Yoshino, Tetsuya Harada, Kazuko Seto, Tetsuya Ikeda, Hiroaki Miyanohara, Koichiro Nakayama, Mutsuyo Gokuden, Naoko Imuta, Hideki Kawamura, Yoshitoshi Ogura, Tetsuya Hayashi, Junichiro Nishi . A Salmonella enterica serovar Oranienburg clone caused a cluster of bacteremia cases in persons with no recognizable underlying diseases in Japan. . Open Forum Infectious Diseases10 ( 1 ) ofac695 2022年12月査読国際誌

担当区分：筆頭著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: Salmonella enterica subspecies enterica serovar Oranienburg (SO) is a foodborne pathogen but rarely causes systemic infections such as bacteremia. Between July and September 2018, bacteremia cases caused by SO were identified in 12 persons without any underlying medical conditions in the southern Kyushu area of Japan. METHODS: Randomly amplified polymorphic DNA (RAPD) analysis was performed to investigate the genetic similarity of the 12 bacteremia-related strains and other Japanese isolates. Furthermore, a series of whole-genome sequence (WGS)-based phylogenetic analyses was performed with a global SO strain set (n = 1648). RESULTS: The resolution power of RAPD was insufficient to investigate the genetic similarity between the bacteremia-related strains and other strains. WGS-based phylogenetic analyses revealed that the bacteremia-related strains formed a tight cluster along with 2 strains isolated from asymptomatic carriers in 2018 in the same area, with a maximum within-cluster single-nucleotide polymorphism (SNP) distance of 11. While several strains isolated in the United States and the United Kingdom were found to be closely related to the bacteremia-related strains, 2 strains isolated in 2016 in the southern Kyushu area were most closely related, with SNP distances of 4-11 and 5-10, and had the same plasmids as the bacteremia-related strains. CONCLUSIONS: The 12 bacteremia cases identified were caused by a single SO clone. As none of the bacteremia patients had any underlying diseases, this clone may be prone to cause bacteremia. Although further analyses are required to understand its virulence, particular attention should be given to this clone and its close relatives in the surveillance of nontyphoidal salmonellae.

DOI： 10.1093/ofid/ofac695

PubMed

Sakura Arai, Tadasuke Ooka, Mizuha Shibata, Yuhki Nagai, Yuki Tokoi, Hiromi Nagaoka, Rika Maeda, Akihiko Tsuchiya, Yuka Kojima, Kenji Ohya, Takahiro Ohnishi, Noriko Konishi, Kayoko Ohtsuka, Yukiko Hara-Kudo . Development of a novel real-time polymerase chain reaction assay to detect Escherichia albertii in chicken meat . Foodborne Pathogens and Disease19 ( 12 ) 823 - 829 2022年12月査読国際誌

Koji Nakae, Tadasuke Ooka, Koichi Murakami, Yukiko Hara-Kudo, Naoko Imuta, Yasuhiro Gotoh, Yoshitoshi Ogura, Tetsuya Hayashi, Yasuhiro Okamoto, and Junichiro Nishi . Diversification of Escherichia albertii H-antigens and development of H-Genotyping PCR . Frontiers in Microbiology12 737979 - 737979 2021年11月査読国際誌

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli. As E. albertii sometimes causes outbreaks of gastroenteritis, rapid strain typing systems, such as the O- and H-serotyping systems widely used for E. coli, will be useful for outbreak investigation and surveillance. Although an O-genotyping system has recently been developed, the diversity of E. albertii H-antigens (flagellins) encoded by fliC genes remains to be systematically investigated, and no H-serotyping or genotyping system is currently available. Here, we analyzed the fliC genes of 243 genome-sequenced E. albertii strains and identified 73 sequence types, which were grouped into four clearly distinguishable types designated E. albertii H-genotypes 1-4 (EAHg1-EAHg4). Although there was a clear sign of intraspecies transfer of fliC genes in E. albertii, none of the four E. albertii H-genotypes (EAHgs) were closely related to any of the 53 known E. coli H-antigens, indicating the absence or rare occurrence of interspecies transfer of fliC genes between the two species. Although the analysis of more E. albertii strains will be required to confirm the low level of variation in their fliC genes, this finding suggests that E. albertii may exist in limited natural hosts or environments and/or that the flagella of E. albertii may function in a limited stage(s) in their life cycle. Based on the fliC sequences of the four EAHgs, we developed a multiplex PCR-based H-genotyping system for E. albertii (EAH-genotyping PCR), which will be useful for epidemiological studies of E. albertii infections.

DOI： 10.3389/fmicb.2021.737979

PubMed

八尋俊輔, 原田誠也, 小原敦美, 藺牟田直子, 大岡唯祐, 西順一郎 . 熊本県におけるβ-ラクタム系薬耐性腸内細菌目細菌の保菌状況調査（2015年4月～2020年3月） . 感染症学雑誌97 ( 5 ) 153 - 161 2023年9月査読

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：一般社団法人日本感染症学会

熊本県におけるβ-ラクタム系薬耐性腸内細菌目細菌（β-lactam antibiotics-resistant Enterobacterales；BLARE）の腸管内保菌状況を把握するため，2015年4月～2020年3月間に当所に搬入された糞便1,434検体から菌分離を試みたところ，340検体（23.7%）から354株のBLAREが分離された．このうち343株（96.9%）は大腸菌であり，ESBL産生大腸菌が305株（86.2%）を占めた．さらに，その中の133株（43.6%）は世界的な拡散が報告されているパンデミック・クローン群の大腸菌O25b-ST131と判定されたことから，このクローン群の拡大が熊本県における薬剤耐性菌腸管内保菌率増加の一因であることが示唆された．なお，分離されたESBL産生菌はすべてCTX-M型遺伝子保有株であり，CTX-M-9 groupが210株（67.7%），CTX-M-1 groupが80株（25.8%），およびCTX-M1とCTX-M-9の両group保有株が13株（4.2%）などであった．また，分離株を検査対象施設の大まかな種類別に集計したところ，乳幼児施設での分離率が35%を超え，複数の施設でESBL産生大腸菌の水平伝播が確認されたことから，乳幼児施設はBLAREのリザーバーの一つである可能性が高いと思われた．

DOI： 10.11150/kansenshogakuzasshi.e21037

Hideki Kawamura, Naoko Imuta, Tadasuke Ooka, Akari Shigemi, Masatoshi Nakamura, Kimiko Mougi, Yuki Obama, Ryuko Fukuyama, Syoko Arimura, Nao Murata, Hiroyuki Tominaga, Hiromi Sasaki, Satoshi Nagano, Noboru Taniguchi, Junichiro Nishi . Impact of control measures including decolonization and hand hygiene for orthopaedic surgical site infection caused by methicillin-resistant Staphylococcus aureus at a Japanese tertiary-care hospital: evaluation of the transmission process by epidemiological and molecular analyses. . Journal of Hospital InfectionS0195-6701(23) 00240-2 2023年7月査読

Keiji Nakamura, Kazuko Seto, Kenichi Lee, Tadasuke Ooka, Yasuhiro Gotoh, Itsuki Taniguchi, Yoshitoshi Ogura, Jacques Georges Mainil, Denis Piérard, Tetsuya Harada, Yoshiki Etoh, Saori Ueda, Mitsuhiro Hamasaki, Junko Isobe, Keiko Kimata, Hiroshi Narimatsu, Jun Yatsuyanagi, Makoto Ohnishi, Sunao Iyoda, Tetsuya Hayashi . Global population structure, genomic diversity and carbohydrate fermentation characteristics of clonal complex 119 (CC119), an understudied Shiga toxin-producing E. coli (STEC) lineage including O165:H25 and O172:H25. . Microbial Genomics9 ( 3 ) 000959 2023年3月査読国際共著国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Microbiology Society

<jats:p>Among Shiga toxin (Stx)-producing <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3093" xlink:type="simple">Escherichia coli</jats:ext-link>
</jats:named-content>
</jats:italic> (STEC) strains of various serotypes, O157:H7 and five major non-O157 STEC (O26:H11, O111:H8, O103:H2, O121:H19 and O145:H28) can be selectively isolated by using tellurite-containing media. While human infections by O165:H25 STEC strains have been reported worldwide, their detection and isolation are not easy, as they are not resistant to tellurite. Systematic whole-genome sequencing (WGS) analyses have not yet been conducted. Here, we defined O165:H25 strains and their close relatives, including O172:H25 strains, as clonal complex 119 (CC119) and performed a global WGS analysis of the major lineage of CC119, called CC119 <jats:italic>sensu stricto</jats:italic> (CC119ss), by using 202 CC119ss strains, including 90 strains sequenced in this study. Detailed comparisons of 13 closed genomes, including 7 obtained in this study, and systematic analyses of Stx phage genomes in 50 strains covering the entire CC119ss lineage, were also conducted. These analyses revealed that the Stx2a phage, the locus of enterocyte effacement (LEE) encoding a type III secretion system (T3SS), many prophages encoding T3SS effectors, and the virulence plasmid were acquired by the common ancestor of CC119ss and have been stably maintained in this lineage, while unusual exchanges of Stx1a and Stx2c phages were found at a single integration site. Although the genome sequences of Stx2a phages were highly conserved, CC119ss strains exhibited notable variation in Stx2 production levels. Further analyses revealed the lack of SpLE1-like elements carrying the tellurite resistance genes in CC119ss and defects in rhamnose, sucrose, salicin and dulcitol fermentation. The genetic backgrounds underlying these defects were also clarified.</jats:p>

DOI： 10.1099/mgen.0.000959

Yusuke Nomoto, Yuko Higashi, Youhei Uchida, Kazuyasu Fujii, Tadasuke Ooka, Takuro Kanekura . Disseminated cutaneous sporotrichosis with intravascular granuloma . The Journal of Dermatology49 ( 9 ) 301 - 302 2022年9月査読

Ruriko Nishida, Keiji Nakamura, Itsuki Taniguchi, Kazunori Murase, Tadasuke Ooka, Yoshitoshi Ogura, Yasuhiro Gotoh, Takehiko Itoh, Atsushi Toyoda, Jacques Georges Mainil, Denis Piérard, Kazuko Seto, Tetsuya Harada, Junko Isobe, Keiko Kimata, Yoshiki Etoh, Mitsuhiro Hamasaki, Hiroshi Narimatsu, Jun Yatsuyanagi, Mitsuhiro Kameyama, Yuko Matsumoto, Yuhki Nagai, Jun Kawase, Eiji Yokoyama, Kazuhiko Ishikawa, Takayuki Shiomoto, Kenichi Lee, Dongchon Kang, Koichi Akashi, Makoto Ohnishi, Sunao Iyoda and Tetsuya Hayashi . The global population structure and evolutionary history of the acquisition of major virulence factor-encoding genetic elements in Shiga toxin-producing Escherichia coli O121:H19 . Microbial Genomics7 ( 12 ) 000716 2021年12月査読国際共著国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）

Shiga toxin (Stx)-producing Escherichia coli (STEC) are foodborne pathogens causing serious diseases, such as haemorrhagic colitis and haemolytic uraemic syndrome. Although O157:H7 STEC strains have been the most prevalent, incidences of STEC infections by several other serotypes have recently increased. O121:H19 STEC is one of these major non-O157 STECs, but systematic whole genome sequence (WGS) analyses have not yet been conducted on this STEC. Here, we performed a global WGS analysis of 638 O121:H19 strains, including 143 sequenced in this study, and a detailed comparison of 11 complete genomes, including four obtained in this study. By serotype-wide WGS analysis, we found that O121:H19 strains were divided into four lineages, including major and second major lineages (named L1 and L3, respectively), and that the locus of enterocyte effacement (LEE) encoding a type III secretion system (T3SS) was acquired by the common ancestor of O121:H19. Analyses of 11 complete genomes belonging to L1 or L3 revealed remarkable interlineage differences in the prophage pool and prophage-encoded T3SS effector repertoire, independent acquisition of virulence plasmids by the two lineages, and high conservation in the prophage repertoire, including that for Stx2a phages in lineage L1. Further sequence determination of complete Stx2a phage genomes of 49 strains confirmed that Stx2a phages in lineage L1 are highly conserved short-tailed phages, while those in lineage L3 are long-tailed lambda-like phages with notable genomic diversity, suggesting that an Stx2a phage was acquired by the common ancestor of L1 and has been stably maintained. Consistent with these genomic features of Stx2a phages, most lineage L1 strains produced much higher levels of Stx2a than lineage L3 strains. Altogether, this study provides a global phylogenetic overview of O121:H19 STEC and shows the interlineage genomic differences and the highly conserved genomic features of the major lineage within this serotype of STEC.

DOI： 10.1099/mgen.0.000716

PubMed

Sakura Arai, Satoko Yamaya, Kayoko Ohtsuka, Noriko Konishi, Hiromi Obata, Tadasuke Ooka, Shouhei Hirose, Akemi Kai, Yukiko Hara-Kudo . Detection of Escherichia albertii in retail oysters . Journal of Food Protection 2021年9月査読

Nguyen Thi Thu Huong, Atsushi Iguchi, Ritsuko Ohata, Hisahiro Kawai, Tadasuke Ooka, Hiroshi Nakajima, Sunao Iyoda . Distribution of novel Og-types in Shiga toxin-producing Escherichia coli isolated from healthy cattle . Journal of Clinical Microbiology59 ( 3 ) e02624-20 2020年12月査読

Ja-Young Lee, Hidehisa Shimizu, Masahito Hagio, Satoru Fukiya, Masamichi Watanabe, Yasutake Tanaka, Ga-Hyun Joe, Hitoshi Iwaya, Reika Yoshitsugu, Keidai Kikuchi, Misaki Tsuji, Nanako Baba, Takuma Nose, Koji Tada, Taketo Hanai, Shota Hori, Akari Takeuchi, Yumiko Furukawa, Bungo Shirouchi, Masao Sato, Tadasuke Ooka, Yoshitoshi Ogura, Tetsuya Hayashi, Atsushi Yokota, and Satoshi Ishizuka . 12α-hydroxylated bile acid induces hepatic steatosis with dysbiosis in rats . BBA Molecular and Cell Biology of Lipids1865 ( 12 ) 158811 2020年12月査読

Fabiano T Romão, Fernando Henrique Martins, Rodrigo Tavanelli Hernandes, Tadasuke Ooka, Fernanda Fernandes Dos Santos, Denise Yamamoto, Alexis Bonfim-Melo, Tetsuya Hayashi, Waldir P Elias, Vanessa Sperandio, Tânia Aparecida Tardelli Gomes . Genomic properties and temporal analysis of the interaction of an invasive Escherichia albertii with epithelial cells . Frontiers Cellular and Infection Microbiology10 571088 2020年12月査読

上村晃秀, 眞鍋佳月, 山本真実, 岩切忠文, 濱田結花, 本田俊郎, 川村英樹, 藺牟田直子, 大岡唯祐, 西順一郎 . 薬剤耐性菌検索体制の整備に関する研究(第III報) . 鹿児島県環境保健センター所報 ( 21 ) 65 - 68 2020年12月

佐藤空見子，永井章子，小原準，遠藤千春，林哲也，大岡唯祐，瀬戸順次，村上光一 . 山形県内と畜場搬入豚のEscherichia albertii保菌状況及びその疫学的特徴 . 日本獣医師会雑誌73 ( 5 ) 265 - 273 2020年6月査読

Tetsuya Ikeda, Toshie Shinagawa, Takuya Ito, Yuta Ohno, Akiko Kubo, Junichiro Nishi, Yasuhiro Gotoh, Yoshitoshi Ogura, Tadasuke Ooka, Tetsuya Hayashi . Hypoosmotic stress induces flagellar biosynthesis and swimming motility in Escherichia albertii. . Communications Biology3 87 2020年2月査読

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