Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1292/jvms.12-0570

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1128/IAI.00851-09

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1128/IAI.01767-06

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4049/jimmunol.179.12.8372.

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1196/annals.1355.026.

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/j.1348-0421.2004.tb03476.x.

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1128/IAI.71.8.4717-4723.2003.

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s12223-023-01071-x

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/1348-0421.13065

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

Anaplasmosis and ehrlichiosis are tick-borne rickettsial diseases that cause significant economic losses in the livestock industry worldwide. Although bovine anaplasmosis is known to be endemic in the Philippines, epidemiological data is fragmented. Moreover, little is known about bovine ehrlichiosis in the country. In this study, the prevalence of Anaplasma marginale and Ehrlichia in cattle and water buffalo from provinces in the southern part of Luzon, Philippines, was investigated through PCR. Blood samples from 620 animals comprised of 512 cattle and 108 water buffalo and 195 tick samples were subjected to nested PCR targeting the groESL gene of Anaplasmataceae. Positive samples were further subjected to another nested PCR and conventional PCR to amplify the A. marginale groEL gene and the Ehrlichia dsbA gene, respectively. Selected A. marginale-positive samples were also subjected to nested PCR targeting the msp5 gene. Regardless of the animal host, the overall prevalence in blood samples obtained was 51.9% for Anaplasmataceae, 43% for A. marginale, and 1.1% for Ehrlichia. No water buffalo were positive for Ehrlichia. Meanwhile, 15.9, 6.7, and 2% of the tick samples, all morphologically identified as Rhipicephalus (Boophilus) microplus, were positive for Anaplasmataceae, A. marginale, and Ehrlichia, respectively. Sequence analysis of selected A. marginale msp5 amplicons showed that the isolates from the region share 94-98% identity to reported A. marginale from other countries. The phylogenetic tree showed clustering of isolates in the region and a close relationship with A. marginale isolates from other countries. Sequences of Ehrlichia amplicons from cattle and ticks were 97-100% similar to reported Ehrlichia minasensis isolates. This study showed the high prevalence of A. marginale in Luzon, Philippines, and provided the first molecular evidence of E. minasensis in the country.

DOI： 10.3389/fvets.2021.746705

DOI： 10.3389/fvets.2021.746705

PubMed

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s10344-020-01424-8

Language：English   Publishing type：Research paper (international conference proceedings)  

Rickettsia and Coxiella burnetii are zoonotic, tick-borne pathogens that can cause febrile illnesses with or without other symptoms in humans, but may cause subclinical infections in animals. There are only a few reports on the occurrence of these pathogens in cattle and water buffalo in Southeast Asia, including the Philippines. In this study, molecular detection of Rickettsia and C. burnetii in the blood and in the Rhipicephalus (Boophilus) microplus ticks of cattle and water buffalo from five provinces in Luzon Island of the Philippines was done. A total of 620 blood samples of cattle and water buffalo and 206 tick samples were collected and subjected to DNA extraction. After successful amplification of control genes, nested PCR was performed to detect gltA of Rickettsia and com1 of C. burnetii. No samples were positive for Rickettsia, while 10 (cattle = 7, water buffaloes = 3), or 1.6% of blood, and five, or 1.8% of tick samples, were C. burnetii-positive. Sequence analysis of the positive amplicons showed 99-100% similarity to reported C. burnetii isolates. This molecular evidence on the occurrence of C. burnetii in Philippine ruminants and cattle ticks and its zoonotic nature should prompt further investigation and surveillance to facilitate its effective control.

DOI： 10.3390/tropicalmed5020054

PubMed

Language：Japanese   Publishing type：Research paper (bulletin of university, research institution)   Publisher：秋田県健康環境センター  

1992年4月〜2011年3月に秋田県で確認された紅斑熱群(SFG)リケッチア感染症に関連したマダニ刺咬3症例について検討した。症例1は虫体が廃棄されていたため検索できなかったものの、発病前の活動歴からベクターは雄物川支流河川敷に生息していたマダニによる媒介が推定された。症例2と3はともにduplex real-time PCRで、SFGリケッチアが検出され、conventional PCRで増幅されたgltA領域341bpにおいてR.helvetica(U59723)と100%の一致を示し、17kDa蛋白遺伝子394pbにおいてはR.helvetica(AJ427881)との相同性は99%で1塩基の置換があった。これにより、両症例を刺咬したヒトツトゲマダニはR.helveticaを保有していたことが確認された。

Language：English   Publishing type：Research paper (scientific journal)  

INTRODUCTION: Murine Eimeria spp. have been used as effective experimental models of disease instead of large mammalian hosts such as cattle. We here examine drug susceptibility of the uncharacterized murine intestinal protozoan parasite, Eimeria krijgsmanni. MATERIALS AND METHODS: The effectiveness of different treatments against infection of E. krijgsmanni was examined for suppression of oocyst shedding: ST mixture ST mixture, pyrimethamine, Ektecin and toltrazuril. RESULTS: ST mixture and pyrimethamine did not suppress oocyst shedding effectively. Although therapeutic efficacy of Ektecin was demonstrated, the dose required was larger than that for cattle and chickens. Oocyst shedding was only completely suppressed completely by continuous administration of toltrazuril. Furthermore, it was confirmed through morphological examination that early developmental stage zoites appeared in host epithelial cells during and following treatment by toltrazuril, and toltrazuril could not eliminate residual zoites in epithelial cells. CONCLUSION: E. krijgsmanni may be relatively resistant to these anti-coccidian agents and might therefore have different characteristics that differ from other coccidia with regard to drug susceptibility.

DOI： 10.2478/s11686-019-00052-w

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1038/s41598-018-37836-5

Language：Japanese   Publishing type：Research paper (bulletin of university, research institution)   Publisher：秋田県健康環境センター  

近隣県である宮城、青森、新潟では近年、紅斑熱群リケッチア症患者が確認されているが、秋田県ではこれまでに患者発生報告はない。本症は、症状がつつが虫病と酷似するため、両者は鑑別が必要な疾患に挙げられているが、これまで当センターにおいては、つつが虫病疑い例に対する紅斑熱群リケッチア症の検査は未実施であった。そこで、本県における紅斑熱群リケッチア症の潜在性を探るため、1992年4月〜2011年3月に臨床的につつが虫病が疑われながらも抗体検査において否定された123例197検体を対象に、血清抗体価測定による後方視的調査を実施した。その結果、過去に紅斑熱群リケッチア症患者が県内で発生していたことが判明した。患者は1995年4月に発病し、つつが虫病疑いとして治療され、治癒していた。これまで、秋田県を含めた東北地方におけるリケッチア症は、つつが虫病が代表的であったが、今後は紅斑熱群リケッチア症の啓発をつつが虫病と併せて実施すると共に、検査体制の整備について検討する必要があると思われる。(著者抄録)

DOI： 10.7601/mez.69.49

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1186/s13071-018-3192-y

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jip.2018.07.005

Web of Science

PubMed

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：The Japan Society of Medical Entomology and Zoology  

<p>Three human cases of tick bite were examined to investigate the occurrence of spotted fever group (SFG) rickettsia in Akita Prefecture. The first case was a 36-year-old male in 1995 with SFG rickettsiosis-like symptoms, such as fever, rash and eschar, who was strongly suspected of being bitten by an adult tick but the tick species was unidentified. Although the etiology remained undetermined at that time, spotted fever group rickettsia infection was definitely diagnosed based on serological diagnosis in 2015. The second case was a 69-year-old female in 2014, and the third one was a 75-year-old female in 2016. They were bitten by a single female tick of <i>Ixodes monospinosus</i>, with <i>Rickettsia helvetica</i> identified genetically. The ticks of both cases were removed early (within 2 days) after tick bite and the patients were administered minocycline prophylactically. They showed no SFG rickettsiosis-like symptoms. Since a patient of SFG rickettsiosis and two <i>R. helvetica</i>-gene positive ticks have been found in Akita Prefecture, the actual situation should be elucidated to take measures in the future.</p>

DOI： 10.7601/mez.69.49

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER GMBH  

Borrelia miyamotoi, recently recognized as a human pathogenic spirochete, was isolated from Ixodes persulcatus and I. ovatus in northern Mongolia and Honshu Island, a major island in Japan. Although no human B. miyamotoi infections have been reported in Mongolia, the prevalence of B. miyamotoi in ticks from Mongolia is higher than that in ticks from Hokkaido, Japan, where human cases have been reported. Moreover, the multi-locus sequence analysis of cultured isolates revealed that B. miyamotoi isolates in Mongolia belong to the Siberian type, a sequence type that was originally reported from isolates from I. persulcatus in Hokkaido. Thus, there is a possibility of unrecognized human B. miyamotoi infections in Mongolia. Moreover our data support the hypothesis of clonal expansion of the Siberian type B. miyamotoi. In contrast, although the isolates were found to belong to the Siberian type B. miyamotoi, two isolates from I. persulcatus in Honshu Island were identified to be of a different sequence type. Furthermore, B. miyamotoi isolates from I. ovatus were distinguishable from those from I. ricinus complex ticks, according to genetic analysis. In this study, we show that there may be some genetic diversity among B. miyamotoi in ticks from Honshu Island.

DOI： 10.1016/j.ttbdis.2017.06.011

Web of Science

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER GMBH, URBAN & FISCHER VERLAG  

To reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (PCR) system to detect the most common agents of tick-borne parasitic diseases, namely Babesia, Theileria, and Hepatozoon parasites. We collected host-seeking or animal-feeding ticks in Kagoshima Prefecture, the southern-most region of Kyusyu Island in southwestern Japan. Twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6 kbp) for the 18S rRNA genes in the novel nested PCR (20/776: 2.58%). These PCR products have individual sequences of Babesia spp. (from 8 ticks), Theileria spp. (from 9 ticks: one tick sample including at least two Theileria spp. sequences), and Hepatozoon spp. (from 3 ticks). Phylogenetic analyses revealed that these sequences were close to those of undescribed Babesia spp. detected in feral raccoons in Japan (5 sequences; 3 sequences being identical), Babesia gibsoni-like parasites detected in pigs in China (3 sequences; all sequences being identical), Theileria spp. detected in sika deer in Japan and China (10 sequences; 2 sequences being identical), Hepatozoon cans (one sequence), and Hepatozoon spp. detected in Japanese martens in Japan (two sequences). In summary, we showed that various tick-borne parasites exist in Kagoshima, the southern region in Japan by using the novel nested PCR system. These including undescribed species such as Babesia gibsoni-like parasites previously detected in pigs in China. Importantly, our results revealed new combinations of ticks and protozoan parasites in southern Japan. The results of this study will aid in the recognition of potential parasitic animal diseases caused by tick-borne parasites.

DOI： 10.1016/j.ttbdis.2017.03.007

Web of Science

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：OXFORD UNIV PRESS  

To demonstrate the effect of climatic elements on Campylobacter colonization in broiler chickens reared in Japan, the correlation between Campylobacter isolated from chickens (191 of 236 flocks, 80.9%) between 2008 and 2012 and climatic elements was analyzed by logistic regression. We divided the rearing process into 13 terms of 5 d each (total: 65 d). Terms were numbered backwards, wherein a 0-term lag was considered as the sampling day plus 4 d before sampling; 1-term lag was the 5-d term before the 0-term lag, and so on, until the 12-term lag. We obtained climatic data tracing back from the 0-term to the 12-term lags. For evaluation in each season, we divided chickens reared during periods of rising temperature (spring, summer) and decreasing temperature (autumn, winter). Air temperature showed a positive correlation with Campylobacter colonization from the 0-to 12-term lags in chickens reared during the period of rising temperature (odds ratio [OR], 1.069 to 1.104), and from the 0-to 4-and 6-term lags (OR, 1.079 to 1.105) in chickens reared during the period of decreasing temperature. The strong positive effect of air temperature on Campylobacter colonization, particularly during the period of rising temperature, may be associated with the effect on the Campylobacter environmental sources and/ or vectors. A positive correlation was observed between Campylobacter colonization and humidity when chicken houses were empty and new chicks were introduced (from the 9-to 12-term lags) during the period of decreasing temperature (OR, 1.076 to 1.141). Thus, high humidity would be an important factor causing carry-over of Campylobacter infection during the period of decreasing temperature. We also found that solar radiation increased Campylobacter colonization during the period of decreasing temperature, from the 2-to 8-term lags, except for the 4-and 5-term lags, in Japan. The results of this study demonstrate the effects of air temperature, humidity, and solar radiation on Campylobacter colonization in broiler chickens, and are potentially important for developing strategies to reduce the risk of Campylobacter contamination in broiler chickens.

DOI： 10.3382/ps/pew354

Web of Science

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/lam.12616

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pone.0104532

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.7601/mez.65.13

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1292/jvms.13-0162

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1017/S0950268811001762

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1292/jvms.11-0110

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1128/IAI.01141-08

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1128/IAI.01609-07

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1128/JCM.41.4.1747-1749.2003.

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/j.1348-0421.2004.tb03627.x.

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1292/jvms.66.1289.

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1128/IAI.70.8.4747-4749.2002.

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/s0168-1702(00)00166-0

Language：English  

The emergence and spread of antimicrobial-resistant bacteria and resistance genes pose serious human and animal health concerns. Therefore, to control antimicrobial-resistant bacteria in the environment, the status of antimicrobial resistance of Escherichia coli in a variety of wild mammals and their prevalence were examined using antimicrobial-containing media. In total, 750 isolates were obtained from 274/366 (74.9%) wild mammals, and antimicrobial-resistant E. coli was detected in 37/750 isolates (4.9%) from 7 animal species (26/366 [7.1%] individuals). Using antimicrobial-containing media, 14 cefotaxime (CTX)- and 35 nalidixic acid-resistant isolates were obtained from 5 (1.4%) and 17 (4.6%) individuals, respectively. CTX-resistant isolates carried blaCTX-M-27, blaCTX-M-55, blaCTX-M-1, and blaCMY-2, with multiple resistance genes. Fluoroquinolone-resistant isolates had multiple mutations in the quinolone-resistance determining regions of gyrA and parC or qnrB19. Most resistant isolates exhibited resistance to multiple antimicrobials. The prevalence of antimicrobial-resistant bacteria observed in wild mammals was low; however, it is essential to elucidate the causative factors related to the low prevalence and transmission route of antimicrobial-resistant bacteria/resistance genes released from human activities to wild animals and prevent an increase in their frequency.

DOI： 10.1292/jvms.22-0415

PubMed

Language：English  

Anaplasmosis and ehrlichiosis are tick-borne rickettsial diseases that cause significant economic losses in the livestock industry worldwide. Although bovine anaplasmosis is known to be endemic in the Philippines, epidemiological data is fragmented. Moreover, little is known about bovine ehrlichiosis in the country. In this study, the prevalence of Anaplasma marginale and Ehrlichia in cattle and water buffalo from provinces in the southern part of Luzon, Philippines, was investigated through PCR. Blood samples from 620 animals comprised of 512 cattle and 108 water buffalo and 195 tick samples were subjected to nested PCR targeting the groESL gene of Anaplasmataceae. Positive samples were further subjected to another nested PCR and conventional PCR to amplify the A. marginale groEL gene and the Ehrlichia dsbA gene, respectively. Selected A. marginale-positive samples were also subjected to nested PCR targeting the msp5 gene. Regardless of the animal host, the overall prevalence in blood samples obtained was 51.9% for Anaplasmataceae, 43% for A. marginale, and 1.1% for Ehrlichia. No water buffalo were positive for Ehrlichia. Meanwhile, 15.9, 6.7, and 2% of the tick samples, all morphologically identified as Rhipicephalus (Boophilus) microplus, were positive for Anaplasmataceae, A. marginale, and Ehrlichia, respectively. Sequence analysis of selected A. marginale msp5 amplicons showed that the isolates from the region share 94-98% identity to reported A. marginale from other countries. The phylogenetic tree showed clustering of isolates in the region and a close relationship with A. marginale isolates from other countries. Sequences of Ehrlichia amplicons from cattle and ticks were 97-100% similar to reported Ehrlichia minasensis isolates. This study showed the high prevalence of A. marginale in Luzon, Philippines, and provided the first molecular evidence of E. minasensis in the country.

DOI： 10.3389/fvets.2021.746705

PubMed

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (other)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (other)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese   Publishing type：Rapid communication, short report, research note, etc. (bulletin of university, research institution)  

Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (other)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Event date： 2021.4

Language：Japanese   Presentation type：Oral presentation (general)  

Event date： 2020.1

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：滋賀  

Event date： 2019.9 - 2019.10

Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

Venue：茨城  

Event date： 2019.9 - 2019.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：茨城  

Event date： 2019.5 - 2019.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：熊本  

Event date： 2019.4

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山口  

Event date： 2019.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京  

Event date： 2018.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京  

Event date： 2018.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京  

Event date： 2018.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京  

Event date： 2018.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：大阪  

Event date： 2018.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：茨城（つくば国際会議場）  

Event date： 2018.3

Language：English   Presentation type：Poster presentation  

Venue：福岡（福岡国際会議場）  

Event date： 2017.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京（国立感染症研究所）  

Event date： 2017.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島（県医師会館）  

Event date： 2017.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島（奄美市AiAi広場）  

Event date： 2017.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：国立感染症研究所  

Event date： 2017.9

Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

Venue：鹿児島大学  

Event date： 2017.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島大学  

Event date： 2016.12

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Venue：国立感染症研究所  

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：神奈川（日本大学生物資源学部）  

Event date： 2016.4

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：栃木（栃木県総合文化センター）  

Event date： 2015.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京（国立感染症研究所）  

Event date： 2015.2

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Venue：鹿児島市（黎明館）  

Event date： 2013.9

Language：Japanese  

Venue：岐阜  

国内学会

Language：Japanese