Updated on 2024/05/25

写真a

 
Kozue Sakao
 
Organization
Research Field in Agriculture, Agriculture, Fisheries and Veterinary Medicine Area Faculty of Agriculture Department of Agriculture Associate Professor
Title
Associate Professor

Degree

  • 博士（農学） ( 2009.9   鹿児島大学 )

Research Interests

  • 有機合成

  • 食品化学

  • 有機化学

  • 分析化学

Research Areas

  • Nanotechnology/Materials / Structural organic chemistry and physical organic chemistry

  • Life Science / Molecular biology

  • Others / Others  / 食品機能化学

  • Life Science / Molecular biology

  • Life Science / Bioorganic chemistry

  • Life Science / Pharmaceutical chemistry and drug development sciences

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Education

  • Kagoshima University

    2004.4 - 2009.9

  • Kyushu Institute of Technology

    2004.4 - 2006.3

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    Country： Japan

  • Kyushu Institute of Technology

    2000.4 - 2004.3

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    Country： Japan

Research History

  • Kagoshima University   Associate Professor

    2024.3

  • Kagoshima University   Research Field in Agriculture, Agriculture, Fisheries and Veterinary Medicine Area Faculty of Agriculture Department of Biochemical Science and Technology   Assistant Professor

    2013.4 - 2024.2

Professional Memberships

  • （公社）日本食品科学工学会

    2023.3

  • 日本農芸化学会

    2015.10

  • 日本フードファクター学会（JSoFF）

    2015.10

  • アメリカ癌学会(AACR)

    2015.10

Studying abroad experiences

  • 2010.2 - 2013.3   ピッツバーグ大学, アメリカ合衆国   ポストドクター

 

Papers

  • Kozue Sakao, Cho SHO, Takeshi Miyata, Kensaku Takara, Rio Oda, De-Xing Hou .  Verification of In Vitro Anticancer Activity and Bioactive Compounds in Cordyceps Militaris-Infused Sweet Potato Shochu Spirits .  Molecules29 ( 9 )   2024.5Invited Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/molecules29092119

    PubMed

  • Kido M, Yoshimoto M, Sakao K, Wada K, Hou DX .  Effects of Cooking Methods on Caffeoylquinic Acids and Radical Scavenging Activity of Sweet Potato. .  Foods (Basel, Switzerland)13 ( 7 )   2024.4Effects of Cooking Methods on Caffeoylquinic Acids and Radical Scavenging Activity of Sweet Potato.Invited Reviewed

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    Language：English  

    DOI： 10.3390/foods13071101

    PubMed

  • Mukai R, Okuyama H, Uchimura M, Sakao K, Matsuhiro M, Ikeda-Imafuku M, Ishima Y, Nishikawa M, Ikushiro S, Tai A .  The binding selectivity of quercetin and its structure-related polyphenols to human serum albumin using a fluorescent dye cocktail for multiplex drug-site mapping. .  Bioorganic chemistry145   107184   2024.4The binding selectivity of quercetin and its structure-related polyphenols to human serum albumin using a fluorescent dye cocktail for multiplex drug-site mapping.Invited Reviewed

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    Language：English  

    DOI： 10.1016/j.bioorg.2024.107184

    PubMed

  • Mizuki Nakamura, Daigo Urakawa, Ziyu He, Isao Akagi, De-Xing Hou, Kozue Sakao .  Apoptosis Induction in HepG2 and HCT116 Cells by a Novel Quercetin-Zinc (II) Complex: Enhanced Absorption of Quercetin and Zinc (II) .  International Journal of Molecular Sciences24 ( 24 )   2023.12Invited Reviewed

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ijms242417457

    PubMed

  • Kozue Sakao, Hanako Saruwatari, Shohei Minami, De-Xing Hou .  Hydroxyl Group Acetylation of Quercetin Enhances Intracellular Absorption and Persistence to Upregulate Anticancer Activity in HepG2 Cells. .  International journal of molecular sciences24 ( 23 )   2023.11Hydroxyl Group Acetylation of Quercetin Enhances Intracellular Absorption and Persistence to Upregulate Anticancer Activity in HepG2 Cells.Invited Reviewed International journal

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Quercetin, a flavonoid compound widely distributed in many plants, is known to have potent antitumor effects on several cancer cells. Our previous study revealed that the acetylation of quercetin enhanced its antitumor effect. However, the mechanisms remain unknown. This study aimed to elucidate the bioavailability of acylated quercetin in the HepG2 cell model based on its antitumor effect. The positions of quercetin 3,7,3',4'-OH were acetylated as 3,7,3',4'-O-tetraacetylquercetin (4Ac-Q). The inhibitory effect of 4Ac-Q on HepG2 cell proliferation was assessed by measuring cell viability. The apoptosis was characterized by apoptotic proteins and mitochondrial membrane potential shifts, as well as mitochondrial reactive oxygen species (ROS) levels. The bioavailability of 4Ac-Q was analyzed by measuring the uptake and metabolites in HepG2 cells with high performance liquid chromatography (HPLC)-photodiode array detector (PDA) and-ultraviolet/visible detector (UV/Vis). The results revealed that 4Ac-Q enhanced the inhibitory effect on HepG2 cell proliferation and induced its apoptosis significantly higher than quercetin. Protein array analysis of apoptosis-related protein indicated that 4Ac-Q increased the activation or expression of pro-apoptotic proteins, including caspase-3, -9, as well as second mitochondria-derived activator of caspases (SMAC), and suppressed the expression of apoptosis inhibiting proteins such as cellular inhibitor of apoptosis (cIAP)-1, -2, Livin, Survivin, and X-linked inhibitor of apoptosis (XIAP). Furthermore, 4Ac-Q stimulated mitochondrial cytochrome c release into the cytosol by enhancing ROS level and depolarizing the mitochondrial membrane. Finally, the analysis of uptake and metabolites of 4Ac-Q in HpG2 cells with HPLC-PDA and -UV/Vis revealed that 4Ac-Q was metabolized to quercetin and several different acetylated quercetins which caused 2.5-fold higher quercetin present in HepG2 cells than parent quercetin. These data demonstrated that acetylation of the quercetin hydroxyl group significantly increased its intracellular absorption. Taken together, our findings provide the first evidence that acetyl modification of quercetin not only substantially augments the intracellular absorption of quercetin but also bolsters its metabolic stability to elongate its intracellular persistence. Therefore, acetylation could serve as a strategic approach to enhance the ability of quercetin and analogous flavonoids to suppress cancer cell proliferation.

    DOI： 10.3390/ijms242316652

    PubMed

  • Nakano H, Sakao K, Wada K, Hou DX .  Ameliorative Effects of Anthocyanin Metabolites on Western Diet-Induced NAFLD by Modulating Co-Occurrence Networks of Gut Microbiome. .  Microorganisms11 ( 10 )   2023.9Ameliorative Effects of Anthocyanin Metabolites on Western Diet-Induced NAFLD by Modulating Co-Occurrence Networks of Gut Microbiome.Invited Reviewed

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    Language：English  

    DOI： 10.3390/microorganisms11102408

    PubMed

  • Tsukasa Orita, Satoshi Chogahara, Mayuko Okuda, Kozue Sakao, Takeshi Miyata, De-Xing Hou .  Extraction Efficiency and Alpha-Glucosidase Inhibitory Activities of Green Tea Catechins by Different Infusion Methods. .  Foods (Basel, Switzerland)12 ( 13 )   2023.7Invited Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    Alpha-glucosidase is an important target for glycemic control with the aim of reducing the risk of type 2 diabetes (T2D). Green tea catechins have been reported to inhibit alpha-glucosidase activity as a potential beverage to control blood glucose levels. However, the effects of the daily infusion style of green tea on tea catechins and their activity remain unclear. In this study, the extraction efficiency of catechins was investigated for 12 green tea extracts (GTEs) infused with 70% ethanol (70% EtOH for 24 h, a favored solvent for catechin extraction), room temperature water infusion (RT H2O for 24 h, an easy way to drink tea), and hot water infusion (Hot H2O for 90 s, a standard way to drink tea). Eight catechins were quantified by HPLC, and the inhibitory effect of GTEs and their catechins on alpha-glucosidase was measured with both rat intestinal enzymes and human Caco-2 cells. The inhibitory mechanism was further analyzed in silico by docking catechins to human alpha-glucosidase using Molecular Operating Environment software. The results showed that total catechins and gallate catechins were efficiently extracted in the order of 70% EtOH, RT H2O, and Hot H2O, and the inhibitory activity against alpha-glucosidase also followed a similar order. Pearson correlation analysis indicated that the alpha-glucosidase inhibitory activity of GTEs was significantly positively correlated with the contents of total catechins, especially gallate catechins. Gallate catechins, such as EGCg and ECg, showed lower IC50 values than free catechins for the enzyme in both rats and humans. In silico simulation revealed that gallate catechins were bound to the different sites with free catechins, and the docking energy of gallate catechins was lower than that of free catechins. Taken together, our data indicated that the daily infusion style of green tea significantly impacted the extraction efficiency and alpha-glucosidase inhibitory activities of catechins, which will give us insight into the use of green tea catechins for glycemic control through efficient infusion.

    DOI： 10.3390/foods12132611

    PubMed

  • Hironobu Nakano, Sho Setoguchi, Kuniaki Kawano, Hiroshi Miyagawa, Kozue Sakao, De-Xing Hou .  Effects of Amazake Produced with Different Aspergillus on Gut Barrier and Microbiota. .  Foods (Basel, Switzerland)12 ( 13 )   2023.6Invited Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract. To explore the preventive effects of dietary foods on IBD, we evaluated the effects of the traditional Japanese fermented beverage "Amazake" on gut barrier function in this study. Black koji Amazake (BA) derived from Aspergillus luchuensis MEM-C strain and yellow koji Amazake (YA) derived from Aspergillus oryzae were made in this study, and their nutrients were analyzed. Mice with mild gut barrier dysfunction induced by Western diet were administered with 10% of each Amazake for two months. Mice gut microbiota were analyzed by 16S rRNA gene sequencing. BA contained a higher amount of isomaltooligosaccharides, citric acid, and ferulic acid than YA. The animal data revealed that BA significantly induced the expressions of antioxidant factors and enzymes such as NF-E2-related factor 2 (Nfr2), heme oxygenase 1 (HO1), and superoxide dismutase-2 (SOD-2). The gut barrier protein, occludin, and fecal immunoglobulin A (IgA) were also significantly enhanced by BA. Furthermore, the levels of serum endotoxin and hepatic monocyte chemotactic protein-1 (MCP-1) were decreased in both the BA and YA groups. In gut microbiota, Lachnospiraceae was increased by BA while Akkermansia muciniphilia was increased by YA. Black koji Amazake contained a higher amount of isomaltooligosaccharides, citric acid, and ferulic acid than yellow koji Amazake and contributed to protecting gut barrier function to reduce endotoxin intrusion and inflammation.

    DOI： 10.3390/foods12132568

    PubMed

  • Yi S, Chen K, Sakao K, Ikenaga M, Wang Y, Hou DX .  Assessment of Areca Nut Bioactivities in Western Diet-Induced Mice NAFLD Model. .  Nutrients15 ( 10 )   2023.5Assessment of Areca Nut Bioactivities in Western Diet-Induced Mice NAFLD Model.Invited Reviewed

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    Language：English  

    DOI： 10.3390/nu15102403

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  • Shuhan Yi, Luyan Zou, Zongjun Li, Kozue Sakao, Yuanliang Wang, De-Xing Hou .  In Vitro Antioxidant Activity of Areca Nut Polyphenol Extracts on RAW264.7 Cells .  Foods11 ( 22 ) 3607 - 3607   2022.11Invited Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Chewing areca nuts is a popular hobby in the Asian region, and areca nuts are rich in polyphenols, although some alkaloids are included. In this study, we explored the antioxidant activity of areca nut polyphenols (ANP) in lipopolysaccharides (LPS)-stimulated RAW264.7 cells. The results revealed that ANP reduced the level of reactive oxygen species (ROS) in LPS-stimulated RAW264.7 cells and enhanced the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1). RNA-seq analysis showed that ANP down-regulated the transcription of genes related to the cancer pathway at 160 μg/mL, and the inflammatory pathway as well as viral infection pathway at 320 μg/mL. The cellular signaling analysis further revealed that the expressions of these genes were regulated by the mitogen-activated protein kinase (MAPK) pathway, and ANP downregulated the activation of the MAPK signaling pathway stimulated by LPS. Collectively, our findings showed that ANP inhibited the MAPK pathway and activated the Nrf2/HO-1 antioxidant pathways to reduce ROS generation induced by LPS.

    DOI： 10.3390/foods11223607

    PubMed

  • Takeshi Miyata, Kosuke Minamihata, Koichi Kurihara, Yui Kamizuru, Mari Gotanda, Momoka Obayashi, Taiki Kitagawa, Keita Sato, Momoko Kimura, Kosuke Oyama, Yuta Ikeda, Yukihiro Tamaki, Jae Man Lee, Kozue Sakao, Daisuke Hamanaka, Takahiro Kusakabe, Mayumi Tachibana, Hisham R Ibrahim .  Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis. .  Protein expression and purification195-196   106096 - 106096   2022.8Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis.Invited Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    Plasmodium vivax ookinete surface protein, Pvs25, is a candidate for a transmission-blocking vaccine (TBV) for malaria. Pvs25 has four EGF-like domains containing 22 cysteine residues forming 11 intramolecular disulfide bonds, a structural feature that makes its recombinant protein expression difficult. In this study, we report the high expression of recombinant Pvs25 as a soluble form in silkworm, Bombyx mori. The Pvs25 protein was purified from hemolymphs of larvae and pupae by affinity chromatography. In the Pvs25 expressed by silkworm, no isoforms with inappropriate disulfide bonds were found, requiring no further purification step, which is necessary in the case of Pichia pastoris-based expression systems. The Pvs25 from silkworm was confirmed to be molecularly uniform by sodium dodecyl sulfate gel electrophoresis and size-exclusion chromatography. To examine the immunogenicity, the Pvs25 from B. mori was administered to BALB/c mice subcutaneously with oil adjuvant. The Pvs25 produced by silkworm induced potent and robust immune responses, and the induced antisera correctly recognized P. vivax ookinetes in vitro, demonstrating the potency of Pvs25 from silkworm as a candidate for a malaria TBV. To the best of our knowledge, this is the first study to construct a system for mass-producing malaria TBV antigens using silkworm.

    DOI： 10.1016/j.pep.2022.106096

    PubMed

  • Keyu Chen, Yasushi Nakasone, Shuhan Yi, Hisham R Ibrahim, Kozue Sakao, Md Amzad Hossain, De-Xing Hou .  Natural Garlic Organosulfur Compounds Prevent Metabolic Disorder of Lipid and Glucose by Increasing Gut Commensal Bacteroides acidifaciens. .  Journal of agricultural and food chemistry70 ( 19 ) 5829 - 5837   2022.5Natural Garlic Organosulfur Compounds Prevent Metabolic Disorder of Lipid and Glucose by Increasing Gut Commensal Bacteroides acidifaciens.Invited Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    A number of reports of the effects of garlic on gut microbiota revealed that the active garlic organosulfur compounds (OSCs) are destabilized by the action of alliinase during garlic preparation. In this study, garlic alliinase was deactivated to obtain stable garlic OSCs. Experiments with C57BL/6J mice fed with lipid and glucose metabolic disorder-inducing Western diet (WD) revealed that stable garlic OSCs prevented the disorder by increasing the relative abundance of gut Bacteroides acidifaciens. Molecular analysis indicated that garlic OSCs inhibited dyslipidemia and fatty liver by increasing taurine and subsequently promoting hepatic fatty acid β-oxidation. In parallel, garlic OSCs could meliorate glucose homeostasis by inhibiting dipeptidyl peptidase-4 (DPP-4) and hepatic gluconeogenesis. In vitro bacterial culture experiments revealed that garlic OSCs directly increased the growth of gut Bacteroides acidifaciens. The results of this study demonstrate that the molecular mechanism of the preventive effect of garlic OSCs on the WD-induced metabolic disorder is attributed to the enhanced growth of Bacteroides acidifaciens and the consequent increase in taurine.

    DOI： 10.1021/acs.jafc.2c00555

    PubMed

  • Xuchi Pan, Kun Xie, Keyu Chen, Ziyu He, Kozue Sakao, De-Xing Hou .  Involvement of AMP-activated Protein Kinase α/Nuclear Factor (Erythroid-derived 2) Like 2-iniatived Signaling Pathway in Cytoprotective Effects of Wasabi 6-(Methylsulfinyl) Hexyl Isothiocyanate. .  Journal of cancer prevention27 ( 1 ) 58 - 67   2022.3Involvement of AMP-activated Protein Kinase α/Nuclear Factor (Erythroid-derived 2) Like 2-iniatived Signaling Pathway in Cytoprotective Effects of Wasabi 6-(Methylsulfinyl) Hexyl Isothiocyanate.Invited Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    6-(Methylsulfinyl) hexyl isothiocyanate (6-MSITC) is an active ingredient present in Wasabi, which is a popular pungent spice used in Japanese cuisine. Our previous studies suggested that the primary antioxidant activity of 6-MSITC may link to other biological activity. This study aimed to clarify how the antioxidant activity of 6-MSITC contributes to preventing overloaded lipid stress in hepatic cell model. HepG2 cells were treated with 6-MSITC at defined concentrations and times in normal medium or in combined fatty acids (CFA) medium, and the targeted proteins were detected by Western blotting. The kinetic data revealed that 6-MSITC activated AMP-activated protein kinase α (AMPKα) and nuclear factor (erythroid-derived 2) like 2 (Nrf2), and then enhanced the protein expression of Forkhead box protein O1 (FOXO1) and Sirtuin1 as well as that of the Nrf2 target proteins, NAD(P)H:quinone oxidoreductase 1 (NQO1) and heme oxygenase (HO-1). Furthermore, lipid metabolic stress was mimicked in HepG2 cells by overloading CFA. 6-MSITC significantly alleviated CFA-induced formation of thiobarbituric acid reactive substances and fat accumulation. Signaling analysis data revealed that 6-MSITC enhanced phosphorylation of AMPKα, upregulated the expression of Nrf2, NQO1, heme oxygenase 1, FOXO1, and Siruin1, and downregulated the expression of PPARα. Taken together, our results suggested that the AMPKα/Nrf2-mediated signaling pathways might be involved in the cytoprotective effects of Wasabi 6-MSITC against metabolic lipid stress.

    DOI： 10.15430/JCP.2022.27.1.58

    PubMed

  • Nakamura, K, Shimamoto, S, Yamamoto, M, Sakao, K, Ohtsuka, A, Ijiri, D .  Effects of supplementation with thinned Tankan (Citrus tankan Hayata) on the lipid peroxidation, drip-loss, and color of the pectoralis major muscle of broiler chickens . .  Journal of Warm Regional Society of Animal Science65 ( 1 ) 17 - 24   2022.3Effects of supplementation with thinned Tankan (Citrus tankan Hayata) on the lipid peroxidation, drip-loss, and color of the pectoralis major muscle of broiler chickens .Invited Reviewed

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  • NAKAMURA Kiriko, SHIMAMOTO Saki, YAMAMOTO Masashi, SAKAO Kozue, OHTSUKA Akira, IJIRI Daichi .  Effects of Supplementation with Thinned Tankan (<i>Citrus tankan</i> Hayata) on the Lipid Peroxidation, Drip-loss, and Color of the <i>Pectoralis Major</i> Muscle of Broiler Chickens .  Nihon Danchi Chikusan Gakkaihou65 ( 1 ) 17 - 24   2022Effects of Supplementation with Thinned Tankan (<i>Citrus tankan</i> Hayata) on the Lipid Peroxidation, Drip-loss, and Color of the <i>Pectoralis Major</i> Muscle of Broiler ChickensInvited Reviewed

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    Language：English   Publisher：Warm Regional Society of Animal Science, Japan  

    <p>This study was conducted to evaluate the effects of dietary supplementation with dried thinned Tankan (<i>Citrus tankan </i>Hayata) powder on the lipid peroxidation, drip-loss, and color of the <i>pectoralis major</i> muscle of broiler chickens. Thirty male broiler chickens (Ross 308) were allocated to three groups at 14 days of age. The control group was fed a standard diet and the remaining two groups were fed the standard diet supplemented with thinned Tankan<i> </i>at concentrations of 2.0% or 4.0% until 28 days of age. The body mass, body mass gain, feed intake, and the tissue masses of the broilers did not differ among the groups, but the 4.0% thinned Tankan-supplemented diet was associated with a higher feed conversion ratio than the control group. Feeding either the 2.0% or 4.0% thinned Tankan<i>-</i>supplemented diet was associated with redder <i>pectoralis major</i> muscle and a higher muscle α-tocopherol concentration than the control chickens. Furthermore, feeding the 4.0% thinned Tankan<i>-</i>supplemented diet was associated with lower muscle malondialdehyde content and lower drip-loss than the control chickens. Thus, thinned Tankan<i> </i>supplementation changes the color, increases the α-tocopherol concentration, and reduces the lipid peroxidation and drip-loss of the meat of broiler chickens.</p>

    DOI： 10.11461/jwaras.65.17

  • 坂尾 こず枝 .  Effects of Supplementation with Thinned Tankan (Citrus tankan Hayata) on the Lipid Peroxidation, Drip-loss, and Color of the Pectoralis Major Muscle of Broiler Chickens. .  Journal of Warm Regional Society of Animal Science-   2022Effects of Supplementation with Thinned Tankan (Citrus tankan Hayata) on the Lipid Peroxidation, Drip-loss, and Color of the Pectoralis Major Muscle of Broiler Chickens.Invited Reviewed

  • Nakano H, Wu S, Sakao K, Hara T, He J, Garcia S, Shetty K, Hou DX .  Bilberry Anthocyanins Ameliorate NAFLD by Improving Dyslipidemia and Gut Microbiome Dysbiosis. .  Nutrients12 ( 11 )   2020.10Bilberry Anthocyanins Ameliorate NAFLD by Improving Dyslipidemia and Gut Microbiome Dysbiosis.

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  • Kun Xie, Xi He, Keyu Chen, Kozue Sakao, De-Xing Hou .  Ameliorative effects and molecular mechanisms of vine tea on western diet-induced NAFLD .  Food Function11 ( 7 ) 5976 - 5991   2020.7Ameliorative effects and molecular mechanisms of vine tea on western diet-induced NAFLDInvited Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d0fo00795a.

  • Xie K, He X, Chen K, Sakao K, Hou DX .  Ameliorative effects and molecular mechanisms of vine tea on western diet-induced NAFLD. .  Food & function11 ( 7 ) 5976 - 5991   2020.7

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    Language：English   Publisher：Royal Society of Chemistry (RSC)  

    <p>VTP might prevent WD-induced NAFLD by balancing fatty acid oxidation and lipogenesis, hepatic oxidative stress, and the gut microbiome.</p>

    DOI： 10.1039/d0fo00795a

    PubMed

  • Chen K, Nakasone Y, Xie K, Sakao K, Hou DX. .  Modulation of Allicin-Free Garlic on Gut Microbiome .  Molecules.   2020.2Modulation of Allicin-Free Garlic on Gut Microbiome

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  • Chen K, Nakasone Y, Xie K, Sakao K, Hou DX .  Modulation of Allicin-Free Garlic on Gut Microbiome. .  Molecules (Basel, Switzerland)25 ( 3 )   2020.2Modulation of Allicin-Free Garlic on Gut Microbiome.

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  • Xie K, He X, Chen K, Chen J, Sakao K, Hou DX. .  Antioxidant Properties of a Traditional Vine Tea, Ampelopsis grossedentata .  Antioxidants (Basel)   2019.8Antioxidant Properties of a Traditional Vine Tea, Ampelopsis grossedentataReviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Xie K, He X, Chen K, Chen J, Sakao K, Hou DX .  Antioxidant Properties of a Traditional Vine Tea, <i>Ampelopsis grossedentata</i>. .  Antioxidants (Basel, Switzerland)8 ( 8 )   2019.8Antioxidant Properties of a Traditional Vine Tea, <i>Ampelopsis grossedentata</i>.

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  • Chen K, Xie K, Liu Z, Nakasone Y, Sakao K, Hossain A, Hou DX. .  Preventive Effects and Mechanisms of Garlic on Dyslipidemia and Gut Microbiome Dysbiosis. .  Nutrients .   2019.5Preventive Effects and Mechanisms of Garlic on Dyslipidemia and Gut Microbiome Dysbiosis.Reviewed

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  • Chen K, Xie K, Liu Z, Nakasone Y, Sakao K, Hossain A, Hou DX .  Preventive Effects and Mechanisms of Garlic on Dyslipidemia and Gut Microbiome Dysbiosis. .  Nutrients11 ( 6 )   2019.5Preventive Effects and Mechanisms of Garlic on Dyslipidemia and Gut Microbiome Dysbiosis.

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  • Yano S, Wu S, Sakao K, Hou DX .  Involvement of ERK1/2-mediated ELK1/CHOP/DR5 pathway in 6-(methylsulfinyl)hexyl isothiocyanate-induced apoptosis of colorectal cancer cells. .  Bioscience, biotechnology, and biochemistry83 ( 5 ) 960 - 969   2019.5Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    <jats:title>ABSTRACT</jats:title>
    <jats:p>6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC) is a major bioactive compound in Wasabi. Although 6-MSITC is reported to have cancer chemopreventive activities in rat model, the molecular mechanism is unclear. In this study, we investigated the anticancer mechanisms using two types of human colorectal cancer cells (HCT116 p53+/+ and p53−/−). 6-MSITC caused cell cycle arrest in G2/M phase and induced apoptosis in both types of cells in the same fashion. Signaling data revealed that the activation of ERK1/2, rather than p53, is recruited for 6-MSITC-induced apoptosis. 6-MSITC stimulated ERK1/2 phosphorylation, and then activated ERK1/2 signaling including ELK1 phosphorylation, and upregulation of C/EBP homologous protein (CHOP) and death receptor 5 (DR5). The MEK1/2 inhibitor U0126 blocked all of these molecular events induced by 6-MSITC, and enhanced the cell viability in both types of cells in the same manner. These results indicated that ERK1/2-mediated ELK1/CHOP/DR5 pathway is involved in 6-MSITC-induced apoptosis in colorectal cancer cells.</jats:p>
    <jats:p>Abbreviations: CHOP: C/EBP homologous protein; DR5: death receptor 5; ELK1: ETS transcription factor; ERK1/2: extracellular signal-regulated kinase 1/2; JNK: Jun-N-terminal kinase; MAPK: mitogen-activated protein kinase; MEK1/2: MAP/ERK kinase 1/2; 6-MSITC: 6-(methylsulfinyl)hexyl isothiocyanate; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PARP: poly(ADP-ribose) polymerase.</jats:p>

    DOI： 10.1080/09168451.2019.1574206

    PubMed

  • Yano S, Wu S, Sakao K, Hou DX. .  Wasabi 6-(methylsulfinyl)hexyl isothiocyanate induces apoptosis in human colorectal cancer cells through p53-independent mitochondrial dysfunction pathway. .  BioFactors (Oxford, England)   2018.5Wasabi 6-(methylsulfinyl)hexyl isothiocyanate induces apoptosis in human colorectal cancer cells through p53-independent mitochondrial dysfunction pathway. Reviewed

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  • Yano S, Wu S, Sakao K, Hou DX .  Wasabi 6-(methylsulfinyl)hexyl isothiocyanate induces apoptosis in human colorectal cancer cells through p53-independent mitochondrial dysfunction pathway. .  BioFactors (Oxford, England)   2018.5Wasabi 6-(methylsulfinyl)hexyl isothiocyanate induces apoptosis in human colorectal cancer cells through p53-independent mitochondrial dysfunction pathway.

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  • Wu S, Yano S, Chen J, Hisanaga A, Sakao K, He X, He J, Hou DX. .  Polyphenols from Lonicera caerulea L. Berry Inhibit LPS-Induced Inflammation through Dual Modulation of Inflammatory and Antioxidant Mediators. .  Journal of Agricultural and Food Chemistry65 ( 25 ) 5133 - 5141   2017.6Polyphenols from Lonicera caerulea L. Berry Inhibit LPS-Induced Inflammation through Dual Modulation of Inflammatory and Antioxidant Mediators.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.jafc.7b01599.

  • Wu S, Yano S, Chen J, Hisanaga A, Sakao K, He X, He J, Hou DX .  Polyphenols from Lonicera caerulea L. Berry Inhibit LPS-Induced Inflammation through Dual Modulation of Inflammatory and Antioxidant Mediators. .  Journal of agricultural and food chemistry65 ( 25 ) 5133 - 5141   2017.6Polyphenols from Lonicera caerulea L. Berry Inhibit LPS-Induced Inflammation through Dual Modulation of Inflammatory and Antioxidant Mediators.

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  • Wu S, Yano S, Hisanaga A, He X, He J, Sakao K, Hou DX .  Polyphenols from Lonicera caerulea L. berry attenuate experimental nonalcoholic steatohepatitis by inhibiting proinflammatory cytokines productions and lipid peroxidation. .  Molecular nutrition & food research61 ( 4 )   2017.4Polyphenols from Lonicera caerulea L. berry attenuate experimental nonalcoholic steatohepatitis by inhibiting proinflammatory cytokines productions and lipid peroxidation.

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  • Trio PZ, Kawahara A, Tanigawa S, Sakao K, Hou DX. .  DNA Microarray Profiling Highlights Nrf2-Mediated Chemoprevention Targeted by Wasabi-Derived Isothiocyanates in HepG2 Cells. .  NUTRITION AND CANCER69 ( 1 ) 105 - 116   2017.2DNA Microarray Profiling Highlights Nrf2-Mediated Chemoprevention Targeted by Wasabi-Derived Isothiocyanates in HepG2 Cells. Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Wu S, Yano S, Hisanaga A, He X, He J, Sakao K, Hou DX. .  Polyphenols from Lonicera caerulea L. berry attenuate experimental nonalcoholic steatohepatitis by inhibiting proinflammatory cytokines productions and lipid peroxidation. .  Molecular Nutrition & Food Research61 ( 4 )   2017.2Polyphenols from Lonicera caerulea L. berry attenuate experimental nonalcoholic steatohepatitis by inhibiting proinflammatory cytokines productions and lipid peroxidation.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/mnfr.201600858.

  • Trio PZ, Kawahara A, Tanigawa S, Sakao K, Hou DX .  DNA Microarray Profiling Highlights Nrf2-Mediated Chemoprevention Targeted by Wasabi-Derived Isothiocyanates in HepG2 Cells. .  Nutrition and cancer69 ( 1 ) 105 - 116   2017.1DNA Microarray Profiling Highlights Nrf2-Mediated Chemoprevention Targeted by Wasabi-Derived Isothiocyanates in HepG2 Cells.

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  • Trio PZ, Fujisaki S, Tanigawa S, Hisanaga A, Sakao K, Hou DX .  DNA Microarray Highlights Nrf2-Mediated Neuron Protection Targeted by Wasabi-Derived Isothiocyanates in IMR-32 Cells. .  Gene Regulation and Systems Biology10   73 - 83   2016.8DNA Microarray Highlights Nrf2-Mediated Neuron Protection Targeted by Wasabi-Derived Isothiocyanates in IMR-32 Cells.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.4137/GRSB.S39440.

  • Hisanaga A, Mukai R, Sakao K, Terao J, Hou DX .  Anti-inflammatory effects and molecular mechanisms of 8-prenyl quercetin. .  Mol Nutr Food Res.   2016.5Anti-inflammatory effects and molecular mechanisms of 8-prenyl quercetin.Reviewed

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  • Sogo T, Terahara N, Hisanaga A, Kumamoto T, Yamashiro T, Shusong Wu, Sakao K, Hou DX. .  Anti-inflammatory aaaactivity and molecular mechanism of delphinidin 3-sambubioside, a Hibiscus anthocyanin. .  BioFactors41 ( 1 ) 58 - 65   2015.2Anti-inflammatory aaaactivity and molecular mechanism of delphinidin 3-sambubioside, a Hibiscus anthocyanin.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Sakao K, Vyas AR, Chinni S, Amjad AI, Parikh R, Singh SV. .  CXCR4 Is a Novel Target of Cancer Chemopreventative Isothiocyanates in Prostate Cancer Cells. .  Cancer Prev Res   2015.2CXCR4 Is a Novel Target of Cancer Chemopreventative Isothiocyanates in Prostate Cancer Cells.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Sogo T, Kumamoto T, Ishida H, Hisanaga A, Sakao K, Terahara N, Wada K, Hou DX. .  Comparison of the inhibitory effects of delphinidin and its glycosides on cell transformation. .  Planta Med. 81 ( 1 ) 26 - 31   2015.1Comparison of the inhibitory effects of delphinidin and its glycosides on cell transformation. Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Zar PP, Sakao K, Hashimoto F, Morishita A, Fujii M,Wada K, Hou DX. .  Antioxidant and Anti-inflammatory Activities of Loquat (Eriobotrya japonica) Tea. .  The Journal of Functional Foods in Health and Disease3   447 - 461   2014.11Antioxidant and Anti-inflammatory Activities of Loquat (Eriobotrya japonica) Tea.Reviewed

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  • Zar PP, Yano S, Sakao K, Hashimoto F, Nakano T, Fujii M, Hou DX. .  In vitro anticancer activity of loquat tea by inducing apoptosis in human leukemia cells. .  Biosci Biotechnol Biochem.78 ( 10 ) 1731 - 1737   2014.10In vitro anticancer activity of loquat tea by inducing apoptosis in human leukemia cells.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Sehrawat A, Sakao K, Singh SV. .  Notch2 activation is protective against anticancer effects of zerumbone in human breast cancer cells. .  Breast Cancer Res Treat. 146 ( 3 ) 543 - 555   2014.8Notch2 activation is protective against anticancer effects of zerumbone in human breast cancer cells.Reviewed

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  • Arakawa T, Tsuboi T, Sattabongkot J, Sakao K, Torii M, Miyata T .  Tricomponent complex loaded with a mosquito-stage antigen of the malaria parasite induces potent transmission-blocking immunity .  Clinical and Vaccine Immunology 21 ( 4 ) 561 - 569   2014.4Tricomponent complex loaded with a mosquito-stage antigen of the malaria parasite induces potent transmission-blocking immunityReviewed

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  • Zar PP, Morishita A, Hashimoto F, Sakao K, Fujii M, Wada K, Hou DX .  Anti-inflammatory Effects and Molecular Mechanisms of Loquat (Eriobotrya japonica) Tea. .  Journal of Functional Foods6   523 - 533   2014.1Anti-inflammatory Effects and Molecular Mechanisms of Loquat (Eriobotrya japonica) Tea.Reviewed

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  • Hahm ER, Sakao K, Singh SV. .  Honokiol activates reactive oxygen species-mediated cytoprotective autophagy in human prostate cancer cells. .  Prostate. 74 ( 12 ) 1209 - 1221   2014.1Honokiol activates reactive oxygen species-mediated cytoprotective autophagy in human prostate cancer cells.Reviewed

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  • 論文 I-9Zar PPK, Sakao K, Hashimoto F, Morishita A, Fujii M, Wada K, Hou DX .  Antioxidant and anti-inflammatory activities of loquat (Eriobotrya japonica) tea .  Functional Foods in Health and Disease3 ( 11 ) 447 - 461   2013.11Antioxidant and anti-inflammatory activities of loquat (Eriobotrya japonica) teaReviewed

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  • Singh S. V. , Kim S. H. , Sehrawat A , Arlotti J. A. , Hahm E. R. , Sakao K. , Beumer J. H. , Jankowitz R. C. , Chandra-Kuntal K. , Lee J. , Powolny A. A. , Dhir R. .  Biomarkers of phenethyl isothiocyanate-mediated mammary cancer chemoprevention in a clinically relevant mouse model .  The Journal of the National Cancer Institute104 ( 16 ) 1228 - 1239   2012.8Biomarkers of phenethyl isothiocyanate-mediated mammary cancer chemoprevention in a clinically relevant mouse modelReviewed

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  • Sakao K. , Desineni S. , Hahm E. R. , Singh S. V. .  Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture and in vivo .  The Prostate72 ( 10 ) 1104 - 1116   2012.7Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture and in vivoReviewed

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  • Sakao K. , Singh S. V. .  D,L-sulforaphane-induced apoptosis in human breast cancer cells is regulated by the adapter protein p66 Shc .  Journal of Cellular Biochemistry113 ( 2 ) 599 - 610   2012.2D,L-sulforaphane-induced apoptosis in human breast cancer cells is regulated by the adapter protein p66 Shc Reviewed

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  • Kim S. H. , Sehrawat A , Sakao K. , Hahm E. R. , Singh S.V. .  Notch activation by phenyl isothiocyanate attenuates its inhibitory effect on prostate cancer cell migration .  PLoS One6 ( 10 ) e26615   2010.10Notch activation by phenyl isothiocyanate attenuates its inhibitory effect on prostate cancer cell migration Reviewed

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  • Kozue Sakao, Makoto Fujii, De-Xing Hou .  Clarification of the role of quercetin hydroxyl groups in superoxide generation and cell apoptosis by chemical modification .  Biosci Biotechnol Biochem73 ( 9 ) 2048 - 2053   2009.9Clarification of the role of quercetin hydroxyl groups in superoxide generation and cell apoptosis by chemical modificationReviewed

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  • Kozue Sakao, Makoto Fujii, De-Xing Hou .  Acetyl derivate of quercetin increases the sensitivity of human leukemia cells toward apoptosis .  BioFactors35 ( 4 ) 399 - 405   2009.7Acetyl derivate of quercetin increases the sensitivity of human leukemia cells toward apoptosis Reviewed

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▼display all

MISC

  • Effects and Mechanisms of Acylated Flavonoid on Cancer Chemopreventive Activity Reviewed

    Kozue Sakao & De-Xing Hou

    Current Pharmacology Reports   6   286 - 305   2020.9

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)   Publisher：Springer  

  • Chemopreventive functions and molecular mechanisms of garlic organosulfur compounds. Reviewed

    Trio PZ, You S, He X, He J, Sakao K, Hou DX

    Food & Function   2014.1

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Presentations

  • 浦川大吾, 中村瑞樹, 侯徳興, 坂尾こず枝   亜鉛強化タマネギの作出ならびに基礎的機能性評価  

    （公社）日本食品科学工学会第70回記念大会  2023.8 

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    Event date： 2023.8

  • 西村啓矢, 中村瑞樹, 何子煜, 侯德興, 坂尾こず枝   ケルセチン亜鉛錯体の合成および機能性評価  

    2022年度（公社）日本食品科学工学会西日本支部および（公社）日本栄養・食糧学会九州・沖縄支部合同大会  2022.11 

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    Event date： 2022.11

    Presentation type：Oral presentation (general)  

  • 坂尾こず枝   ポリフェノールの化学修飾にともなう生理活性と代謝の変化   Invited

    2022年度（公社）日本食品科学工学会西日本支部および（公社）日本栄養・食糧学会九州・沖縄支部合同大会  2022.11 

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    Event date： 2022.11

    Presentation type：Symposium, workshop panel (nominated)  

  • 小田莉央, 井尻大地, 片渕歩美, 侯德興, 坂尾こず枝   ケルセチン及びケルセチン配糖体の似生体環境下における挙動解明  

    2022年度（公社）日本食品科学工学会西日本支部および（公社）日本栄養・食糧学会九州・沖縄支部合同大会  2022.11 

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    Event date： 2022.11

  • 内村望空, 釘丸朋樹, 山本雅史, 井尻大地, 侯徳興, 坂尾こず枝   カンキツ成分の食用油抽出による、美白効果増強油の簡易作成法  

    第76 回日本栄養・食糧学会大会  2022.6 

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    Event date： 2022.6

    Presentation type：Oral presentation (general)  

  • 中村瑞樹、侯德興、坂尾こず枝   ケルセチン亜鉛錯体の合成および生理活性評価  

    2020年度日本フードファクター学会・日本農芸化学会西日本支部合同大会（第332回講演会）  2020.11 

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    Event date： 2020.11

    Language：Japanese  

    Venue：宮崎大学  

  • 牟禮野乃華、侯德興、坂尾こず枝   ケルセチンのアセチル修飾の位置と数による生理活性の変化  

    2020年度日本フードファクター学会・日本農芸化学会西日本支部合同大会（第332回講演会）  2020.11 

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    Event date： 2020.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：宮崎大学  

  • Garlic as potential prebiotics to prevent gut microbiota dysbiosis and dyslipidemia   International conference

    2020.11 

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    Event date： 2020.11

    Language：English   Presentation type：Oral presentation (general)  

    Venue：online  

  • Keyu Chen, Kozue Sakao, De-Xing Hou   Preventive Effects and Mechanisms of Garlic on Dyslipidemia and Gut Microbiome Dysbiosis.  

    日本農芸化学会2020大会 

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    Event date： 2020.3

    Presentation type：Oral presentation (general)  

  • 牟禮野乃華, 侯徳興, 侯徳興, 坂尾こず枝, 坂尾こず枝   ケルセチンのアセチル修飾の位置と数による生理活性の変化  

    日本農芸化学会西日本支部大会およびシンポジウム講演要旨集  2020 

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    Event date： 2020

  • 山下智代, 坂尾こず枝, 賀喜, 侯徳興   脂質代謝および腸内細菌叢におけるローズマリーの影響に関する研究  

    日本栄養・食糧学会九州・沖縄支部大会講演要旨集  2020 

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    Event date： 2020

  • 中村瑞樹, 侯徳興, 侯徳興, 坂尾こず枝, 坂尾こず枝   ケルセチン亜鉛錯体の合成および生理活性評価  

    日本農芸化学会西日本支部大会およびシンポジウム講演要旨集  2020 

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    Event date： 2020

  • Kozue Sakao   Terpenes induce structure dependent autophagy and apoptosis in human colon cancer cells   Invited International conference

    7th International Conference on Food Factors(ICoFF2019) 

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    Event date： 2019.12

    Language：English   Presentation type：Oral presentation (general)  

  • Kozue Sakao   Clarifying the structure-activity relationship and cancer chemopreventive effects of terpenes in human colon cancer cells.   Invited International conference

    Food for Health International Conference2018,  

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    Event date： 2018.11

    Language：English   Presentation type：Oral presentation (general)  

  • 濱元志穂美, 侯徳興, 侯徳興, 坂尾こず枝, 坂尾こず枝   ヒト乳がん細胞においてQuercetinのアセチル化はアポトーシス誘導を増強する  

    日本フードファクター学会学術集会講演要旨集  2018 

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    Event date： 2018

  • 新村麻弥華, 木戸めぐみ, 木戸めぐみ, 坂尾こず枝, 侯徳興   調理加工における黒豆の機能性の変化に関する研究  

    日本栄養・食糧学会九州・沖縄支部大会講演要旨集  2018 

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    Event date： 2018

  • 栗田志保, 久永絢美, 伍樹松, 矢野敏史, 坂尾こず枝, 坂尾こず枝, 橋本文雄, 橋本文雄, 侯徳興, 侯徳興   茶プルプロガリンの抗炎症機能及び細胞内分子機構に関する研究  

    日本フードファクター学会学術集会講演要旨集  2018 

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    Event date： 2018

  • 楢本龍之介, 高山耕二, 坂尾こず枝, 侯徳興   メタボローム解析による薩摩黒鴨の肉質特徴の解明  

    日本栄養・食糧学会九州・沖縄支部大会講演要旨集  2018 

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    Event date： 2018

  • 猿渡春菜子, 侯徳興, 侯徳興, 坂尾こず枝, 坂尾こず枝   ヒト肝臓がん細胞におけるケルセチンとその誘導体の細胞死誘導メカニズムの解明  

    日本フードファクター学会学術集会講演要旨集  2018 

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    Event date： 2018

  • Kozue Sakao and De-Xing Hou   Synthesis and structure activity relationships of quercetin derivatives against human cancer cells.   Invited International conference

    Food for Health International Conference 2017  Food for Health International Society

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    Event date： 2017.10

    Language：English   Presentation type：Oral presentation (invited, special)  

    Venue： Hunan Agricultural University  

  • Kozue Sakao, Akimi Imamura, Taichi Hara, and De-Xing Hou   Acetylated quercetin enhanced induction of programmed cell death in human colon carcinoma   International conference

    The 8th International Conference on Polyphenols and Health  Laval University

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    Event date： 2017.10

    Language：English   Presentation type：Poster presentation  

    Venue：Quebec convention center  

  • 村上 愛, 坂尾 こず枝, 章 超, 高瀬 良和, 岩井 謙一, 侯 徳興   サツマイモ焼酎により作製したチャーガスピリッツの抗炎症能解析  

    日本栄養・食糧学会大会講演要旨集  2017.4  (公社)日本栄養・食糧学会

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    Event date： 2017.4

    Language：Japanese  

  • 中野拓宜, WU Shusong, 坂尾こず枝, 坂尾こず枝, HOU De-Xing, HOU De-Xing   ビルベリーによる非アルコール性脂肪性肝炎の予防効果及び作用機構  

    日本フードファクター学会学術集会講演要旨集  2017 

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    Event date： 2017

  • 矢野敏史, 坂尾こず枝, 坂尾こず枝, HOU De-Xing, HOU De-Xing   ワサビ機能性成分の抗癌作用および抗癌剤との併用効果の解析  

    日本農芸化学会大会講演要旨集(Web)  2017 

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    Event date： 2017

  • 坂尾こず枝, 侯徳興   アセチル化ケルセチンはヒト乳がん細胞におけるアポトーシス誘導能を増強する  

    第21回日本フードファクター学会 

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    Event date： 2016.11

    Language：English   Presentation type：Oral presentation (general)  

  • K. Sakao, D.X. Hou   Apoptosis induction of quercetin derivatives in human breast cancer cells.   International conference

    The International Conference on Biosciences 2016 

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    Event date： 2016.7

    Language：English   Presentation type：Poster presentation  

  • Kozue Sakao, Akimi Imamura, Taichi Hara, and De-Xing Hou   Quercetin derivatives induced programmed cell death in HCT116 human colon carcinoma.   International conference

    Food for Health International Conference 2016 

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    Event date： 2016.3

    Language：English   Presentation type：Poster presentation  

  • K. Sakao and D.X. Hou   Quercetin derivatives induced p53 independent apoptosis in HCT116 human colon carcinoma   International conference

    International Conference on Polyphenols and Health (ICPH 2015) 

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    Event date： 2015.10

    Language：English   Presentation type：Poster presentation  

    Venue：Tours, France  

  • 章 超, 坂尾 こず枝, 廣 眞梨子, 高瀬 良和, 岩井 謙一, 侯 徳興   芋焼酎のチャーガスピリッツの機能性解析  

    日本生物工学会大会講演要旨集  2015.9  (公社)日本生物工学会

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    Event date： 2015.9

    Language：Japanese  

  • K. Sakao and D.X. Hou   Apoptosis induction of Quercetin derivatives in human colon carcinoma cells   Invited International conference

    International Symposium on Current Advances in Radiobiology, Stem Cells and Cancer Research 

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    Event date： 2015.2

    Language：English  

    Venue：New Delhi, India  

  • Sho Cho, Sakao Kozue, Hiro Mariko, Takase Yoshikazu, Iwai Kenichi, Hou De-Xing   3P-122 The bioactive functions by the extract of Inonotus obilquus soaked in the sweetpotato shochu  

    日本生物工学会大会講演要旨集  2015  日本生物工学会

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    Event date： 2015

    Language：Japanese  

  • Kozue Sakao, Chiyomi Okuyama and De-Xing Hou   In vitro anticancer activity of quercetin derivatives by inducing apoptosis in HCT116 human colon carcinoma.   International conference

    5th Annual Conference of the American Council for Medicinally Active Plants  5th Annual Conference of the American Council for Medicinally Active Plants

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    Event date： 2014.6

    Language：English  

    Venue：Northdacota ・USA  

    国際学会

  • 矢野敏史, 坂尾こず枝, HOU De‐Xing   MEK1/2は死のシグナルとしてワサビ6‐MSITCによるアポトーシスやオートファジーに働く  

    日本フードファクター学会学術集会講演要旨集  2014 

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    Event date： 2014

    Language：Japanese  

  • 畠田優美, 橋本文雄, 坂尾こず枝, HOU De‐Xing   鹿角霊芝は抗癌剤DOXおよび5‐FUとの併用により大腸癌細胞増殖に対して異なる抑制効果を示す  

    日本フードファクター学会学術集会講演要旨集  2014 

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    Event date： 2014

    Language：Japanese  

  • 野屋靖奈, 泊奈緒子, 坂尾こず枝, HOU De‐Xing   誘導体を用いたケルセチンの細胞抗酸化機構の解析  

    日本フードファクター学会学術集会講演要旨集  2014 

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    Event date： 2014

    Language：Japanese  

  • 大脇美香, 入角順平, 坂尾こず枝, HOU De‐Xing   ビルベリーによる非アルコール性脂肪肝の予防効果解析  

    日本フードファクター学会学術集会講演要旨集  2014 

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    Event date： 2014

    Language：Japanese  

  • 山城隆明, 坂尾こず枝, HOU De‐Xing   バイカレインはERKを介したp53の活性化により癌細胞のアポトーシスを誘導する  

    日本フードファクター学会学術集会講演要旨集  2014 

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    Event date： 2014

    Language：Japanese  

  • 乗富紀久子, 後藤梨々子, 森下暁子, 坂尾こず枝, HOU De‐Xing   トリテルペン化合物によるヒト大腸癌細胞の細胞死誘導機序に関する研究  

    日本フードファクター学会学術集会講演要旨集  2014 

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    Event date： 2014

    Language：Japanese  

  • 章超, 章超, 坂尾こず枝, 坂尾こず枝, 矢野敏史, 小野友愛, 久永絢美, 高瀬良和, 岩井謙一, HOU De-Xing, HOU De-Xing   サツマイモ焼酎の冬虫夏草スピリッツの抽出物の抗癌活性  

    日本フードファクター学会学術集会講演要旨集  2014 

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    Event date： 2014

  • 久永絢美, 坂尾こず枝, HOU De‐Xing   サスペンションアレイシステムを用いたケルセチン誘導体による炎症性サイトカインの抑制効果及び分子機構  

    日本フードファクター学会学術集会講演要旨集  2014 

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    Event date： 2014

    Language：Japanese  

  • 黒江宥冶, 坂尾こず枝, HOU De‐Xing   ケルセチン及びその誘導体によるヒト大腸癌細胞のオートファジー誘導機構の解析  

    日本フードファクター学会学術集会講演要旨集  2014 

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    Event date： 2014

    Language：Japanese  

  • 宇都菜央、吉崎由美子、奥津果優、坂尾こず枝、髙峯和則、鮫島吉廣   ヒト結腸がん細胞株Caco-2に対する高温液化麹成分の機能性評価  

    日本応用糖質科学会平成２５年度大会（第６２回）  日本応用糖質科学会平成２５年度大会（第６２回）

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    Event date： 2013.9

    Language：Japanese  

    Venue：鹿児島  

    国内学会

  • 宇都菜央, 吉崎由美子, 奥津果優, 坂尾こず枝, 高峯和則, 鮫島吉廣   ヒト結腸がん細胞株Caco‐2に対する高温液化麹成分の機能性評価  

    応用糖質科学  2013.8  (一社)日本応用糖質科学会

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    Event date： 2013.8

    Language：Japanese  

  • Kozue Sakao, Eun-Ryeong Hahm, Shivendra V. Singh   Chemoprevention of mammary and prostate cancer by phenethyl isothiocyanate in vivo is associated with suppression of vimentin protein expression.  

    CANCER RESEARCH  2013.4  AMER ASSOC CANCER RESEARCH

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    Event date： 2013.4

    Language：English  

  • 入角 順平, 十合 貴之, 坂尾 こず枝, 山本 雅史, 福留 弘康, 川口 昭二, 冨永 茂人, 侯 徳興   Analysis of antioxidant capacity and anthocyanin composition among the cultivars of blueberry (Vaccinium spp.)  

    鹿児島大学農学部学術報告  2013.3  鹿児島大学

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    Event date： 2013.3

    Language：Japanese  

    ブルーベリー(Vaccinium spp.)はアントシアニンを多く含み,機能性食品として注目されている。一方,アントシアニンの組成は品種問で異なると報告されている。本研究では機能性食品利用の視点で,鹿児島大学農学部附属農場唐湊果樹園で栽培されている12品種のブルーベリーについて,溶媒のアントシアニン抽出効率やポリフェノール含量,アントシアニン組成,抗酸化能(DPPH法,ORAC法)を検討した。その結果,50%酢酸一50%エタノールは1%塩酸一99%メタノールとほぼ同等の高い効果を示した。品種間の比較では,ポリフェノール含量,アントシアニン含量,抗酸化能などではジョージアジムが低い値となったが,他の品種間に有意な差は見られなかった。また,ポリフェノール含量と抗酸化能の間には中位・高位の正の相関が認められた。Blueberry (Vaccinium spp.) contains higher amounts of anthocyanins and is reported to be benefit to human health. Onthe other hand, there are differences on amounts and compositions of anthocyanins among the cultivars of blueberry, whichconcerns the functionality. In the present study, we first examined the extract-efficiency of the anthocyanins with the solventsavailable for food utilization, and then investigated anthocyanin compositions and antioxidant capacity (DPPH andORAC) in 12 cultivars of blueberry cultivated in the Toso Orchard, Experimental Farm of the Faculty. The results revealedthat the solvent consisting of 50% acetic acid - 50% ethanol showed the same extract efficiency as that with the solvent of1% hydrochloric acid - 99% methanol, a classic solvent for anthocyanin extracts but not available for food utilization. Thereare no significant differences on the contents of polyphenols and anthocyanins„ and antioxidant capacity except Georgiagemblueberry showed the lower activity. Moreover, positive correlations of middle to high degree were observed between thepolyphenol content and the antioxidant capacity.

  • 宇都 菜央, 吉崎 由美子, 奥津 果優, 坂尾 こず枝, 高峯 和則, 鮫島 吉廣   Dp-8 ヒト結腸がん細胞株Caco-2に対する高温液化麹成分の機能性評価(糖質の生理機能,一般講演,日本応用糖質科学会平成25年度大会(第62回))  

    応用糖質科学：日本応用糖質科学会誌  2013  一般社団法人 日本応用糖質科学会

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    Event date： 2013

    Language：Japanese  

  • 山城隆明, 谷川俊祐, 隈元拓馬, 坂尾こず枝, 候徳興   クルクミンによるアポトーシス誘導はUBE1活性阻害及びp53の安定化が必要である  

    日本分子生物学会年会プログラム・要旨集(Web)  2013 

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    Event date： 2013

    Language：Japanese  

  • 久永絢美、十合貴之、坂尾こず枝、陳継華、伍樹松、侯徳興   Quercetin及びQuercetin改変体の抗炎症作用及び分子機構の解析  

    第17回日本フードファクター学会大会,  第17回日本フードファクター学会大会,

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    Event date： 2012.11

    Language：Japanese  

    Venue：静岡  

    国内学会

  • 赤川郁美、森下暁子、坂尾こず枝、向井理恵、寺尾純二、侯徳興   ヒト大腸癌細胞におけるプレニル化ケルセチンのアポトーシス誘導機構の解析  

    第17回日本フードファクター学会大会  第17回日本フードファクター学会大会

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    Event date： 2012.11

    Language：Japanese  

    Venue：静岡  

    国内学会

  • Sakao K, Desineni S, Hahm ER, Singh SV   Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture and in vivo.   International conference

    AACR 103rd ANNUAL MEETING  AACR 103rd ANNUAL MEETING

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    Event date： 2012.4

    Language：English  

    Venue：米国  

    国際学会

  • Singh SV , Kim SH , Sehrawat A , Arlotti JA , Hahm ER , Sakao K , Beumer JH , Jankowitz RC , Chandra-Kuntal K , Lee J , Powolny AA , Dhir R   Biomarkers of phenethyl isothiocyanate-mediated mammary cancer chemoprevention in a clinically relevant mouse model.   International conference

    AACR 103rd ANNUAL MEETING  AACR 103rd ANNUAL MEETING

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    Event date： 2012.4

    Language：English  

    Venue：米国  

    国際学会

  • Kim SH, Sehrawat A, Sakao K, Hahm ER, Singh SV   Notch activation by phenyl isothiocyanate attenuates its inhibitory effect on prostate cancer cell migration   International conference

    AACR 103rd ANNUAL MEETING  AACR 103rd ANNUAL MEETING

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    Event date： 2012.4

    Language：English  

    Venue：米国  

    国際学会

  • Shivendra V. Singh, Su-Hyeong Kim, Anuradha Sehrawat, Julie A. Arlotti, Kozue Sakao, Joomin Lee, Kumar Chandra-Kuntal, Eun-Ryeong Hahm   Biomarkers of phenethyl isothiocyanate-mediated mammary cancer prevention in MMTV-neu mouse model  

    CANCER RESEARCH  2012.4  AMER ASSOC CANCER RESEARCH

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    Event date： 2012.4

    Language：English  

  • Kozue Sakao, Sudhakar Desineni, Eun-Ryeong Hahm, Shivendra V. Singh   Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture and in vivo  

    CANCER RESEARCH  2012.4  AMER ASSOC CANCER RESEARCH

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    Event date： 2012.4

    Language：English  

  • Su-Hyeong Kim, Anuradha Sehrawat, Kozue Sakao, Eun-Ryeong Hahm, Shivendra V. Singh   Notch1 and Notch2 activation by phenethyl isothiocyanate hinders its inhibitory effect on prostate cancer cell migration  

    CANCER RESEARCH  2012.4  AMER ASSOC CANCER RESEARCH

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    Event date： 2012.4

    Language：English  

  • Julie A. Arlotti, Su-Hyeong Kim, Anuradha Sehrawat, Eun-Ryeong Hahm, Anna A. Powolny, Joomin Lee, Jan H. Beumer, Kozue Sakao, Shivendra V. Singh   Chemoprevention of mammary cancer in MMTV-neu transgenic mice by dietary administration of cruciferous vegetable constituent phenethyl isothiocyanate  

    CANCER RESEARCH  2012.4  AMER ASSOC CANCER RESEARCH

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    Event date： 2012.4

    Language：English  

  • 赤川郁美, 森下暁子, 坂尾こず枝, 向井理恵, 寺尾純二, 候徳興   プレニル化ケルセチンによるヒト大腸癌細胞アポトーシス誘導能の増強  

    日本農芸化学会西日本支部大会およびシンポジウム講演要旨集  2012 

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    Event date： 2012

  • Hiroko Kuwata, Kozue Sakao, Eri Mukai, Junji Terao, Tomoyuki Kawamura, Hisao Nemoto, Jihua Chen, De-Xing Hou   Prenylated quercetin enhances the inhibitory effects on inflammatory mediators   International conference

    International Conference of Food Factor 2011(ICoFF2011)  International Conference of Food Factor 2011(ICoFF2011)

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    Event date： 2011.11

    Language：English  

    Venue：台湾  

    国際学会

  • Kozue Sakao , Singh SV   D,L-Sulforaphane-induced apoptosis in human breast cancer cells is regulated by growth factor adaptor protein p66Shc   International conference

    AACR 102nd ANNUAL MEETING  AACR 102nd ANNUAL MEETING

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    Event date： 2011.4

    Language：English  

    Venue：米国  

    国際学会

  • Kozue Sakao, Shivendra V. Singh   D,L-Sulforaphane-induced apoptosis in human breast cancer cells is regulated by growth factor adaptor protein p66Shc  

    CANCER RESEARCH  2011.4  AMER ASSOC CANCER RESEARCH

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    Event date： 2011.4

    Language：English  

  • 桑田広子、坂尾こず枝、向井理恵、寺尾純二、河村知志、根本尚夫、藤井 信、侯 徳興   プレニル化ケルセチンの機能性解析：炎症性因子の抑制およびその分子機構  

    日本農芸化学会  日本農芸化学会

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    Event date： 2011.3

    Language：Japanese  

    Venue：京都  

    国内学会

  • 桑田広子, 坂尾こず枝, 向井理恵, 寺尾純二, 河村知志, 根本尚夫, 藤井信, 侯徳興   プレニル化ケルセチンの機能性解析:炎症性因子の抑制およびその分子機構  

    日本農芸化学会大会講演要旨集  2011 

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    Event date： 2011

  • 奥山 千代美, 坂尾 こずえ, 藤井 信, 侯 徳興   構造改変によるケルセチンの大腸癌細胞増殖抑制の分子機構の解析  

    第33回日本分子生物学会年会  第33回日本分子生物学会年会

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    Event date： 2010.12

    Language：Japanese  

    Venue：神戸  

    国内学会

  • 奥山 千代美, 坂尾 こず枝, 藤井 信, 侯 徳興   構造改変によるケルセチンの大腸癌細胞増殖抑制の分子機構の解析  

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2010.12  (公社)日本生化学会

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    Event date： 2010.12

    Language：Japanese  

  • 十合貴之、坂尾こず枝、藤井 信、侯 徳興   ケルセチンのヒドロキシル基がMEK1の抑制に関与する  

    第15回日本フードファクター学会  第15回日本フードファクター学会

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    Event date： 2010.10

    Language：Japanese  

    Venue：仙台  

    国内学会

  • 坂尾 こず枝、藤井 信、侯 徳興   化学修飾によるケルセチンの構造活性の解析  

    日本農芸化学会2010年大会  日本農芸化学会2010年大会

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    Event date： 2010.3

    Language：Japanese  

    Venue：東京  

    国内学会

  • Kozue Sakao , Makoto Fujii, De-Xing Hou   Clarification of Structure-Activity Relationship of Quercetin by Chemical Modification   International conference

    The 2nd Joint Meeting of Kagoshima University (Japan) Chosun University (Korea) on Relationship between Protein Activity Control and Natural Products  The 2nd Joint Meeting of Kagoshima University (Japan) Chosun University (Korea) on Relationship between Protein Activity Control and Natural Products

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    Event date： 2009.9

    Language：English  

    Venue：Gwangju, Korea  

    国際学会

  • Kozue Sakao , Makoto Fujii, De-Xing Hou   Chemical modification and the structure-activity relationships of quercetin derivatives  

    日本農芸化学会2009年大会  日本農芸化学会2009年大会

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    Event date： 2009.3

    Language：Japanese  

    Venue：福岡  

    国内学会

  • Kozue Sakao, Mayumi Kubo, Makoto, Fujii, De-Xing Hou   Ampelopsin induces apoptosis in HL-60 cell through ROS-mediated mitochondrial death pathway   International conference

    3rd International Conference on Polyphenols and Health (ICPH2007)  3rd International Conference on Polyphenols and Health (ICPH2007)

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    Event date： 2007.11

    Language：English  

    Venue：京都  

    国際学会

  • Kozue Sakao, Mamoru Hashimoto , Kazunori Sakata   Synthesis and molecular recognition of tetraaza[14]annulene nickel(II) complex dimers bearing asymmetric cavities   International conference

    PACIFICHEM 2005  PACIFICHEM 2005

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    Event date： 2005.12

    Language：English  

    Venue：米国  

    国際学会

  • 坂尾こず枝、 橋本守、 坂田一矩   ピンセット型分子及び不斉場を有するアヌレンの合成と機能評価  

    日本化学会西日本大会  日本化学会西日本大会

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    Event date： 2004.10

    Language：Japanese  

    Venue：大分  

    国内学会

  • 坂尾こず枝、 橋本守、 坂田一矩   ピンセット機能を有するテトラアザ[14]アヌレンの合成と分子認識  

    第41回化学関連支部合同九州大会  第41回化学関連支部合同九州大会

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    Event date： 2004.7

    Language：Japanese  

    Venue：福岡  

    国内学会

  • 村上 愛, 坂尾 こず枝, 章 超, 高瀬 良和, 岩井 謙一, 侯 徳興   サツマイモ焼酎により作製したチャーガスピリッツの抗炎症能解析  

    日本栄養・食糧学会大会講演要旨集  2017.4  (公社)日本栄養・食糧学会

  • 中野 拓宜, 坂尾 こず枝, 侯 徳興   非アルコール性脂肪性肝疾患(NAFLD)におけるビルベリー及びアントシアニン代謝産物による予防効果　腸内細菌叢の視点から  

    日本栄養・食糧学会大会講演要旨集  2022.5  (公社)日本栄養・食糧学会

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    Language：Japanese  

  • 内村 望空, 釘丸 朋樹, 山本 雅史, 井尻 大地, 侯 徳興, 坂尾 こず枝   カンキツ成分の食用油抽出による、美白効果増強油の簡易作成法  

    日本栄養・食糧学会大会講演要旨集  2022.5  (公社)日本栄養・食糧学会

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    Language：Japanese  

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Research Projects

  • Elucidation of the uniqueness, diversity, and usefulness of local citrus such as Shiikuwasha grown on the Ryukyu Islands

    Grant number：22K12544  2022.4 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4030000 （ Direct Cost: \3100000 、 Indirect Cost：\930000 ）

  • Challenging to resolve the paradox in anthocyanin function: Study on the interactions between anthocyanin and gut microbiome

    Grant number：20K05928  2020.4 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）

  • フラボノイド類の乳がん転移予防効果を最大限に活かすには？

    2019.4 - 2022.3

    科学研究費補助金　基盤研究(C) 

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    Authorship：Principal investigator  Grant type：Competitive

  • フラボノイド類の乳がん転移予防効果を最大限に活かすには？

    Grant number：19K05931  2019.4 - 2022.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    坂尾 こず枝, 侯 徳興

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    Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）

    前年度までに実施した、がん細胞の遊走や浸潤に対する抗転移能の各種測定の結果より、高い抗転移効果を示したケルセチンとアセチル化ケルセチンを用いて、より詳細の転移抑制のメカニズムを解明するために、がん細胞転移の重要なプロセスの一つである上皮から間葉への移行（EMT）の活性化の抑制への関与についてウェスタンブロッティング法を用いてタンパク質発現レベルでの解析を行った。結果、ケルセチンならびにアセチル化ケルセチンはMDA-MB-231細胞においてE-cadherinの発現を6時間、24時間で増加させ、Vimentinの発現を24時間で減少させた。また、E-Cadherinの発現を抑制する転写因子Snail1の減少が関与することも明らかになった。これらはPCR解析による結果と一致した。また、抗血管新生効果についてヒト臍帯静脈内皮細胞（HUVEC細胞）を用い、3次元血管新生能評価を実施した。結果、ケルセチンならびにアセチル化ケルセチンはVEGF で誘導したHUVEC の増殖を抑制し、その効果はアセチル化誘導体の方が高かった。また、内皮細胞において膜に局在するCD31がケルセチンならびにアセチル化ケルセチン処理により減少したことから、血管内皮細胞の機能を失ったことが示唆された。これらより、ケルセチンならびにアセチル化ケルセチンが血管新生を抑制することが示唆された。さらに、抗転移効果が高かった3種類のフラボノイドについては、さらにアセチル基の修飾数の異なるアセチル化フラボノイドを新規合成し、抗転移効果との構造相関の解明も試みた。結果、興味深いことに、フラボン類とフラボノール類では、アセチル基の数による抗転移効果への寄与が異なる可能性が示唆された。

  • アセチル化ケルセチンの生理活性増強の原因の解明

    2016.4 - 2018.3

    科学研究費補助金  若手研究(B)

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    Authorship：Principal investigator  Grant type：Competitive

  • ケルセチン誘導体を用いたヒトがん細胞死誘導メカニズムの解明

    2014.4 - 2016.3

    科学研究費補助金  若手研究(B)

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Teaching Experience

  • Molecular Food Science

    2013.4
    Institution：Kagoshima University

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    Level：Undergraduate (specialized)  Country：Japan

 

Social Activities

  • 甲南高校スーパーグローバルハイスクール事業

    Role(s)： Advisor

    2016.6

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    Audience： High school students

    Type：Seminar, workshop

    甲南高校課題研究における指導・助言