Updated on 2022/07/29

写真a

 
Kazuhiro Shiozaki
 
Organization
Research Field in Fisheries, Agriculture, Fisheries and Veterinary Medicine Area Faculty of Fisheries Department of Fisheries Science and Technology Associate Professor
Title
Associate Professor
External link

Degree

  • 博士(農学) ( 2005.3   東北大学 )

Research Interests

  • 小型魚類

  • シアリダーゼ

  • 糖鎖

  • 健康機能性

  • Hypertension

  • Glycoconjugate

  • 胃潰瘍

  • Glycolipid

  • 糖尿病

  • Glycoprotein

  • 水産化学

  • 天然物

  • 大腸

  • Medaka

  • Histamine

  • ティラピア

  • Sialic acid

  • Sialidase

  • Cancer

Research Areas

  • Life Science / Food sciences

  • Life Science / Cell biology

  • Life Science / Aquatic life science

  • Life Science / Functional biochemistry

  • Life Science / Molecular biology

Research History

  • Kagoshima University   Associate Professor

    2015.4

  • Kagoshima University   Faculty of Fisheries, Department of Fisheries   Assistant Professor

    2010.4 - 2015.3

Professional Memberships

  • 日本糖質学会

    2015.10

  • 日本水産学会

    2015.10

 

Papers

  • Ikeda A, Yamasaki C, Kubo Y, Doi Y, Komamizu M, Komatsu M, Shiozaki K .  Alteration of the neuronal and glial cell profiles in Neu1-deficient zebrafish. .  Glycoconjugate journal   2022.7Alteration of the neuronal and glial cell profiles in Neu1-deficient zebrafish.

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    DOI: 10.1007/s10719-022-10074-8

    PubMed

  • Daichi Sahashi, Yurina Kubo, Mika Ishii, Asami Ikeda, Chiharu Yamasaki, Masaharu Komatsu, Kazuhiro Shiozaki .  Neu1 deficiency increases the susceptibility of zebrafish to Edwardsiella piscicida infection via lysosomal dysfunction. .  Gene836   146667 - 146667   2022.6Neu1 deficiency increases the susceptibility of zebrafish to Edwardsiella piscicida infection via lysosomal dysfunction.International journal

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    Neu1 is a lysosomal glycosidase that catalyzes the removal of sialic acids from glycoconjugates. Although Neu1 sialidase is highly conserved among vertebrates, the role of fish Neu1 is not fully understood because of its unique aquatic living situation. Compared to land animals, fish have a higher chance of bacterial infection, and to understand the role of fish Neu1, the susceptibility of Neu1 knockout zebrafish (Neu1-KO) was evaluated using Edwardsiella piscicida, a fish pathogen. Neu1-KO larvae showed high susceptibility to E. piscicida, despite the activation of macrophages, and presented increased lysosomal signals induced by the accumulation of Sia α2-3 linked oligosaccharides. The accumulation coincided with the signal of the macrophage marker, suggesting that the dysfunction of lysosomes in macrophages would result in a high susceptibility of Neu1-KO to E. piscicida. Chloroquine, an inhibitor of lysosomal degradation, induced high mortality of wild type zebrafish with E. piscicida infection accompanied by increased lysosomal accumulation, similar to Neu1-KO zebrafish. This study revealed that Neu1 sialidase plays a crucial role in the lysosomal degradation of macrophages with a bacterial infection.

    DOI: 10.1016/j.gene.2022.146667

    PubMed

  • Linh Khanh Vo, Nhung Thi Tran, Yurina Kubo, Daichi Sahashi, Masaharu Komatsu, Kazuhiro Shiozaki .  Enhancement of Edwardsiella piscicida infection, biofilm formation, and motility caused by N-acetylneuraminate lyase. .  Glycoconjugate journal39 ( 3 ) 429 - 442   2022.6Enhancement of Edwardsiella piscicida infection, biofilm formation, and motility caused by N-acetylneuraminate lyase.International journal

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    Sialic acid and its catabolism are involved in bacterial pathogenicity. N-acetylneuraminate lyase (NAL), which catalyzes the reversible aldol cleavage of sialic acid to form N-acetyl-D-mannosamine in the first step of sialic acid degradation, has been recently investigated to elucidate whether NAL enhances bacterial virulence; however, the role of NAL in bacterial pathogenicity remains unclear. In the present study, we demonstrated that the existence of two enzymes in Edwardsiella piscicida, referred to as dihydrodipicolinate synthase (DHDPS) and NAL, induced the cleavage/condensation activity toward sialic acids such as N-acetylneuraminic acid, N-glycolylneuraminic acid and 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid. NAL enhanced cellular infection in vitro and suppressed the survival rate in zebrafish larvae in bath-infection in vivo, whereas DHDPS did not. Furthermore, NAL strongly activated the expression of E. piscicida phenotypes such as biofilm formation and motility, whereas DHDPS did not. Besides, the gene expression level of nanK, nanE, and glmU were up-regulated in the NAL-overexpressing strain, along with an increase in the total amount of N-acetylglucosamine.

    DOI: 10.1007/s10719-022-10045-z

    PubMed

  • Nhung Thi Tran, Linh Khanh Vo, Masaharu Komatsu, Kazuhiro Shiozaki .  Involvement of N-acetylneuraminate cytidylyltransferase in Edwardsiella piscicida pathogenicity. .  Fish & shellfish immunology124   534 - 542   2022.5Involvement of N-acetylneuraminate cytidylyltransferase in Edwardsiella piscicida pathogenicity.International journal

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    Edwardsiella piscicida is a gram-negative bacterium that causes Edwardsiellosis in cultured fish. Edwardsiellosis is accompanied by symptoms such as skin lesions, hemorrhage, and necrosis in fish organs, which leads to significant economic losses in the aquaculture industry. Recently, we found that bacterial sialoglycoconjugates may be involved in the infectivity of E. piscicida. The more infectious strains of E. piscicida contain more sialic acid in the bacterial body, and the mRNA level of putative CMP-Neu5Ac synthase (css) is upregulated compared to that in the non-pathogenic strain. However, this putative css gene is yet to be cloned, and the involvement of CSS in E. piscicida pathogenicity remains unclear. Here, we cloned and transferred the css gene from E. piscicida into the FPC498 strain. CSS promoted infection in cultured cells originating from different fish species, and enhanced the mortality of E. piscicida-infected zebrafish larvae. CSS enhanced cell attachment and motility in E. piscicida, which differs from the decreased bacterial growth observed with the sialic acid-supplemented M9 medium. Both fractions (chloroform-methanol)-soluble and -insoluble fraction) prepared from E. piscicida pellet exhibited the increment of sialo-conjugates induced by CSS. Further, lectin blotting revealed the increment of Sia α2-3- and α2-6-, but not α2-8-, -linked glycoprotein in CSS-overexpressing E. piscicida. Overall, these findings indicate the physiological significance of CSS and the role of sialylation in E. piscicida pathogenicity.

    DOI: 10.1016/j.fsi.2022.04.033

    PubMed

  • 河辺 ももこ, 堀田 千穂子, 西田 拓未, 久保 友理奈, 小松 正治, 塩﨑 一弘 .  疾患モデルゼブラフィッシュを用いた糖転移ヘスペリジンの抗不安活性解析 .  応用糖質科学:日本応用糖質科学会誌12 ( 1 ) 43 - 44   2022.2疾患モデルゼブラフィッシュを用いた糖転移ヘスペリジンの抗不安活性解析

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    Language:Japanese   Publisher:一般社団法人 日本応用糖質科学会  

    DOI: 10.5458/bag.12.1_43

  • Asami Ikeda, Mayu Komamizu, Akito Hayashi, Chiharu Yamasaki, Keiji Okada, Momoko Kawabe, Masaharu Komatsu, Kazuhiro Shiozaki .  Neu1 deficiency induces abnormal emotional behavior in zebrafish. .  Scientific reports11 ( 1 ) 13477 - 13477   2021.6Neu1 deficiency induces abnormal emotional behavior in zebrafish.International journal

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    NEU1 sialidase hydrolyzes sialic acids from glycoconjugates in lysosomes. Deficiency of NEU1 causes sialidosis with symptoms including facial dysmorphism, bone dysplasia, and neurodegeneration. However, the effects of NEU1 deficiency on emotional activity have not been explored. Here, we conducted the behavioral analysis using Neu1-knockout zebrafish (Neu1-KO). Neu1-KO zebrafish showed normal swimming similar to wild-type zebrafish (WT), whereas shoaling was decreased and accompanied by greater inter-fish distance than WT zebrafish. The aggression test showed a reduced aggressive behavior in Neu1-KO zebrafish than in WT zebrafish. In the mirror and 3-chambers test, Neu1-KO zebrafish showed more interest toward the opponent in the mirror and multiple unfamiliar zebrafish, respectively, than WT zebrafish. Furthermore, Neu1-KO zebrafish also showed increased interaction with different fish species, whereas WT zebrafish avoided them. In the black-white preference test, Neu1-KO zebrafish showed an abnormal preference for the white region, whereas WT zebrafish preferred the black region. Neu1-KO zebrafish were characterized by a downregulation of the anxiety-related genes of the hypothalamic-pituitary-adrenal axis and upregulation of lamp1a, an activator of lysosomal exocytosis, with their brains accumulating several sphingoglycolipids. This study revealed that Neu1 deficiency caused abnormal emotional behavior in zebrafish, possibly due to neuronal dysfunction induced by lysosomal exocytosis.

    DOI: 10.1038/s41598-021-92778-9

    PubMed

  • Kazuki Oishi, Moeri Morise, Linh Khanh Vo, Nhung Thi Tran, Daichi Sahashi, Rena Ueda-Wakamatsu, Wataru Nishimura, Masaharu Komatsu, Kazuhiro Shiozaki .  Host lactosylceramide enhances Edwardsiella tarda infection. .  Cellular microbiology23 ( 9 ) e13365   2021.5Host lactosylceramide enhances Edwardsiella tarda infection.International journal

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    Edwardsiella tarda is a Gram-negative bacterium causing economic damage in aquaculture. The interaction of E. tarda with microdomains is an important step in the invasion, but the target molecules in microdomains remain undefined. Here, we found that intraperitoneal injection of E. tarda altered splenic glycosphingolipid patterns in the model host medaka (Oryzias latipes) accompanied by alteration of glycosphingolipid metabolism-related gene expressions, suggesting that glycosphingolipid levels are involved in E. tarda infection. To ascertain the significance of glycosphingolipids in the infection, fish cell lines, DIT29 cells with a high amount of lactosylceramide (LacCer) and glucosylceramide (GlcCer), and GAKS cells with a low amount of these lipids, were treated with methyl-β-cyclodextrin to disrupt the microdomain. E. tarda infection was suppressed in DIT29 cells, but not in GAKS cells, suggesting the involvement of microdomain LacCer and GlcCer in the infection. DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol, an inhibitor of glycosphingolipid-synthesis, attenuated the infection in DIT29 cells, while Neu3-overexpressing GAKS cells, which accumulated LacCer, enhanced the infection. E. tarda possessed binding ability towards LacCer, but not GlcCer, and LacCer preincubation declined the infection towards fish cells, possibly due to the masking of binding sites. The present study suggests that LacCer may be a positive regulator of E. tarda invasion.

    DOI: 10.1111/cmi.13365

    PubMed

  • Akinobu Honda, Oki Hayasaka, Kenjiro Mio, Koji Fujimura, Tomonari Kotani, Masaharu Komatsu, Kazuhiro Shiozaki .  The involvement of Nile tilapia (Oreochromis niloticus) Neu4 sialidase in neural differentiation during early ontogenesis. .  Biochimie185   105 - 116   2021.3The involvement of Nile tilapia (Oreochromis niloticus) Neu4 sialidase in neural differentiation during early ontogenesis.International journal

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    Neurogenesis is an important process for the formation of the central nervous system during ontogenesis. Mammalian sialidases are involved in neurogenesis through desialylation of sialo-glycoconjugates. However, the significance of fish sialidases, unlike that of mammals, in neurogenesis has not been investigated. The present study focuses on Nile tilapia (Oreochromis niloticus) because of its unique profiles of sialidases related to enzymatic properties, subcellular localization, and tissue-specific gene expression. First, the fish were cultured under aphotic condition, which is known to cause the delayed development of the retina and brain in various fish. Next, we investigate the effect of aphotic condition on the levels of tilapia sialidases. Our results revealed that the tilapia showed a decrease in the number of ganglion cell in the retina. The expression level of neu4 mRNA is up-regulated in the eyes from tilapia reared in Dark accompanied by the increase of retinal differentiation markers. These results indicated that tilapia Neu4 is involved in retinal development in Nile tilapia. Furthermore, we tried to clarify the function of tilapia Neu4 in the neuronal cells using two neuroblast cell lines (SH-SY5Y and Neuro2a cell lines). Tilapia Neu4 decreased sialic acid level of both nuclear glycoproteins as well as glycolipids. Moreover, tilapia Neu4 accelerated neurite formation in both two neural cell lines and, increased the acetylcholinesterase activity, but it did not affect cell proliferation. Collectively, these results suggest that Neu4 accelerates neurite differentiation during ontogenesis in tilapia.

    DOI: 10.1016/j.biochi.2021.03.008

    PubMed

  • Momoko Kawabe, Akito Hayashi, Masaharu Komatsu, Akio Inui, Kazuhiro Shiozaki .  Ninjinyoeito improves anxiety behavior in neuropeptide Y deficient zebrafish. .  Neuropeptides87   102136 - 102136   2021.3Ninjinyoeito improves anxiety behavior in neuropeptide Y deficient zebrafish.International journal

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    Anxiety induced by excess mental or physical stress is deeply involved in the onset of human psychiatric diseases such as depression, bipolar disorder, and panic disorder. Recently, Kampo medicines have received focus as antidepressant drugs for clinical use because of their synergistic and additive effects. Thus, we evaluated the anxiolytic activity of Ninjinyoeito (NYT) using neuropeptide Y-knockout (NPY-KO) zebrafish that exhibit severe anxiety responses to acute stress. Adult NPY-KO zebrafish were fed either a 3% NYT-supplemented or normal diet (i.e., the control diet) for four days and were then examined via behavioral tests. After short-term cold stress (10 °C, 2 s) was applied, control-fed NPY-KO zebrafish exhibited anxiety behaviors such as freezing, erratic movement, and increased swimming time along the tank wall. On the other hand, NYT-fed NPY-KO zebrafish significantly suppressed these anxiety behaviors, accompanied by a downregulation of tyrosine hydroxylase levels and phosphorylation of extracellular signal-regulated kinases in the brain. To understand the responsible component(s) in NYT, twelve kinds of herbal medicines that composed NYT were tested in behavioral trials with the zebrafish. Among them, nine significantly reduced freezing behavior in NPY-KO zebrafish. In particular, Schisandra fruit induced the most potent effect on abnormal zebrafish behavior, even in the lower amount (0.3% equivalent to NYT), followed by Atractylodes rhizome and Cinnamon bark. Subsequently, four lignans uniquely found in Schisandra fruit (i.e., gomisin A, gomisin N, schizandrin, and schizandrin B) were investigated for their anxiolytic activity in NPY-KO zebrafish. As a result, schizandrin was identified as a responsible compound in the anxiolytic effect of NYT. These results suggest that NYT has a positive effect on mental stress-induced anxiety and may be a promising therapeutic for psychiatric diseases.

    DOI: 10.1016/j.npep.2021.102136

    PubMed

  • Tran Nguyen Duy Khoa, Viliame Waqalevu, Akinobu Honda, Hideaki Matsui, Nguyen Xuan Truong, Kenzo Sakaguchi, Hirofumi Kawaji, Manabu Ishikawa, Kazuhiro Shiozaki, Tomonari Kotani .  Enrichment effects of fermented by-product of Shochu distillery on Brachionus plicatilis sp. rotifer and larviculture performance in Japanese flounder (Paralichthys olivaceus) .  AQUACULTURE535   2021.3Enrichment effects of fermented by-product of Shochu distillery on Brachionus plicatilis sp. rotifer and larviculture performance in Japanese flounder (Paralichthys olivaceus)

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    The effective use of distillery by-products of the shochu sweet potato has long been considered as a rich source of protein, fiber, amino acids, fatty acids, probiotics with a great potential as a functional feed for animals. This study applied fermented by-product of Shochu distillery (FBPSD) and salmon roe emulsion oil for rotifer enrichment to evaluate its effects on the: (i) nutritional composition of rotifers and (ii) feeding responses of Paralichthys olivaceus larvae when fed with enriched rotifers. The salmon roe oil enrichment was set as a control diet and FBPSD was supplemented at different doses (1, 5 and 10 % based on the salmon roe oil amount). The results showed that the rotifers supplemented with FBPSD at 5 and 10 % significantly enhanced protein, carbohydrate, and HUFA content. Similarly, the activity and expression profiles of targeted enzymes including trypsin, chymotrypsin, lipase and amylase effectively responded at early and middle-metamorphosis of the larvae to 5 and 10 % enrichment regiment. These two treatments were also found to be better supported in energy metabolism evidenced by Cytochrome-C-Oxidase (cox) and ATPase-pk (atp) gene expression in the larvae. Moreover, the expression levels of immune-related genes (IL-10, IL-1 beta, TNF-alpha, P2Y(6)) and antioxidant-related genes (SOD, CAT) were significantly improved at 5 and 10 % of FBPSD. Cessation of feeding and delay in growth during the metamorphic process was also molecularly demonstrated. Larvae suffered low survival (24.5-25.3 %) in control and 1 % supplementation of FBPSD, but significantly improved at 5 and 10 % (36.4-38.7%). However, the growth performance of larval fish was not remarkable after 40 days of culture. The results of this study revealed that FBPSD could be considered as a potential supplementary enrichment for live feed in fish larviculture, especially at 5 and 10 % rotifer enrichment dosage.

    DOI: 10.1016/j.aquaculture.2021.736352

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  • Tran Nguyen Duy Khoa, Viliame Waqalevu, Akinobu Honda, Kazuhiro Shiozaki, Tomonari Kotani .  An integrative description of the digestive system morphology and function of Japanese flounder (Paralichthys olivaceus) during early ontogenetic development .  AQUACULTURE531   2021.1An integrative description of the digestive system morphology and function of Japanese flounder (Paralichthys olivaceus) during early ontogenetic development

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    A good understanding of the morphological and functional changes during early larval fish ontogeny is essential for the enhancement of larviculture techniques. This study applied an integrative approach to describe the ontogenetic development of the Japanese flounder (Paralichthys olivaceus) emphasizing the changes in histology and related functions of its digestive system. Changes in histological structures of the digestive tract, expression and activity of digestive enzymes (trypsin, chymotrypsin, lipase, pepsin, amylase), expression of energy metabolism (ATP synthase (atp), cytochrome-c-oxidase (cox), regulatory factors (neuropeptide Y (npy), cholecystokinin (cck)) and growth (insulin like growth factor 1 (igf1), growth hormone (GH)) were investigated and linked to larval biometrics. The larvae absorbed nutrients from its yolk upon hatching until mouth opening (3 DAH), before beginning its exogenous nutritional phase. The digestive tract differentiations were histologically observed at 5 DAH. The results showed that the targeted enzymes were genetically programmed and activated from hatching, except pepsin which was detected at 25 DAH coinciding with visible development of the gastric glands. The larval stages (3-5 DAH) were marked with high mortality at exogenous feeding transition and also at the onset of metamorphosis (20-30 DAH). Energy metabolism, growth and peptide regulatory factor related genes were transcribed and translated at hatching to assist upon the onset of exogenous feeding. Less feeding and growth delay were noticed between 20 and 30 DAH. The digestive system was fully developed by 35 DAH and larvae transited to settlement life.

    DOI: 10.1016/j.aquaculture.2020.735855

    Web of Science

  • Tran Nguyen Duy Khoa, Oki Hayasaka, Hideaki Matsui, Viliame Waqalevu, Akinobu Honda, Kentaro Nakajima, Hirofumi Yamashita, Manabu Ishikawa, Kazuhiro Shiozaki, Tomonari Kotani .  Changes in early digestive tract morphology, enzyme expression and activity of Kawakawa tuna (Euthynnus affinis) .  AQUACULTURE530   2021.1Changes in early digestive tract morphology, enzyme expression and activity of Kawakawa tuna (Euthynnus affinis)

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    Kawakawa tuna (Euthynnus affinis) is a novel target species for aquaculture in Japan and currently there is very limited knowledge on its early ontogenetic development in captivity. The ontogeny of E. affinis larvae from hatching to 20 days after hatching (DAH) was illustrated in the present study through an integrated approach of histology, biochemistry and molecular approaches. Larvae were fed enriched L-type rotifer Brachionus plicatilis species complex from 2 to 10 DAH, followed by enriched Artemia nauplii. Target digestive enzymes; trypsin, pepsin, lipase and amylase were assessed in order to evaluate nutritional condition. The larvae began exogenous feeding from 2 DAH, coinciding with the opening of the oral capacity and anus. The yolk sac was rapidly consumed and completely resorbed at 3 DAH, while the oil globule disappeared by 5 DAH. Enzymatic activity and expression of the target enzymes were detectable at hatching, except pepsin. With the development of the gastric glands, pepsin activity was then detected at 10 DAH, which was followed by rapid increase in pepsin activity at 15 DAH. The larvae showed rapid growth, however, high mortality also occurred at 6-10 DAH which has been highlighted as a critical stage in the larviculture of E. affinis. From 15 to 20 DAH, the histological structures of the digestive tract were completely segmented with the digestive tract resembling that of its juvenile stages. The activity of trypsin, pepsin, and lipase tended to increase sharply, while amylase dramatically dropped, possibly indicating a change in dietary preference. Findings from the present study provides a preliminary insight into the early development of the digestive system and associated digestive enzymes of E. affinis larvae and establishes an initial step towards the rearing and feeding protocol optimization of this novel target species.

    DOI: 10.1016/j.aquaculture.2020.735935

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  • Tran, NDK., Waqalevu, V., Honda, A., Shiozaki, K., Kotani, T. .  An integrative description of the digestive system morphology and function of Japanese flounder (Paralichthys olivaceus) during early ontogenetic development .  Aquaculture531   735855   2021An integrative description of the digestive system morphology and function of Japanese flounder (Paralichthys olivaceus) during early ontogenetic developmentReviewed

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  • Shiozaki, K., Uezono, K., Hirai, G., Honda, A., Minoda, M., Wakata R .  Identification of novel fish sialidase genes responsible for KDN-cleaving activity. .  Glycoconjugate Journal   2021 Identification of novel fish sialidase genes responsible for KDN-cleaving activity.Reviewed

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  • Tran, NDK., Hayasaka, O., Matsui, H., Waqalevu, V., Honda, A., Nakajima。K., Yamashita, H., Ishikawa, M., Shiozaki, K., Kotani, T. .  Changes in early digestive tract morphology, enzyme expression and activity of Kawakawa tuna (Euthynnus affinis) .  Aquaculture   2021 Changes in early digestive tract morphology, enzyme expression and activity of Kawakawa tuna (Euthynnus affinis)Reviewed

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  • Kazuhiro Shiozaki, Keiya Uezono, Go Hirai, Akinobu Honda, Masaya Minoda, Ryuta Wakata .  Identification of novel fish sialidase genes responsible for KDN-cleaving activity. .  Glycoconjugate journal37 ( 6 ) 745 - 753   2020.9Identification of novel fish sialidase genes responsible for KDN-cleaving activity.Reviewed International journal

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    2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) is a minor component of sialic acids detected in vertebrates, such as human cancer cells, rat liver, and fish tissues. Although the enzyme activity of KDN-cleaving sialidase (KDN-sialidase) has been detected in rainbow trout, the gene responsible for its expression has not been identified in vertebrates. We evaluated sialidases in human and various fish for their KDN-cleaving activity using an artificial substrate, methylumbelliferyl-KDN (MU-KDN). Four of the human sialidases tested (NEU1, NEU2, NEU3, and NEU4) did not hydrolyze MU-KDN. Although most fish Neu1s showed negligible KDN-sialidase activity, two Neu1b sialidases from Oreochromis niloticus and Astyanax mexicanus, a paralog of Neu1, exhibited a potent KDN-sialidase activity. Further, O. niloticus and Oryzias latipes Neu3a exhibited a drastically high KDN-sialidase activity, while Danio rerio Neu3.1 showed moderate activities and other Neu3 proteins exhibited little activity. All the Neu4 sialidases tested in fish cleaved KDN and Neu5Ac from MU-KDN and MU-Neu5Ac, respectively, with equivalent potential. To our knowledge, this is the first report to identify KDN-sialidase genes in vertebrates and we believe that KDN-sialidase activity could be conserved among fish Neu4s.

    DOI: 10.1007/s10719-020-09948-6

    PubMed

  • Keiji Okada, Ryo Takase, Yurie Hamaoka, Akinobu Honda, Asami Ikeda, Yoichiro Hokazono, Yutaro Maeda, Oki Hayasaka, Tomonari Kotani, Masaharu Komatsu, Kazuhiro Shiozaki .  Establishment and characterization of Neu1-knockout zebrafish and its abnormal clinical phenotypes. .  The Biochemical journal477 ( 15 ) 2841 - 2857   2020.8Reviewed International journal

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    Mammalian sialidase Neu1 is involved in various physiological functions, including cell adhesion, differentiation, cancer metastasis, and diabetes through lysosomal catabolism and desialylation of glycoproteins at the plasma membrane. Various animal models have been established to further explore the functions of vertebrate Neu1. The present study focused on zebrafish (Danio rerio) belonging to Cypriniformes as an experimental animal model with neu1 gene deficiency. The results revealed that the zebrafish Neu1 desialyzed both α2-3 and α2-6 sialic acid linkages from oligosaccharides and glycoproteins at pH 4.5, and it is highly conserved with other fish species and mammalian Neu1. Furthermore, Neu1-knockout zebrafish (Neu1-KO) was established through CRISPR/Cas9 genome editing. Neu1-KO fish exhibited slight abnormal embryogenesis with the accumulation of pleural effusion; however, no embryonic lethality was observed. Although Neu1-KO fish were able to be maintained as homozygous, they showed smaller body length and weight than the wild-type (WT) fish, and muscle atrophy and curvature of the vertebra were observed in adult Neu1-KO fish (8 months). The expression patterns of myod and myog transcription factors regulating muscle differentiation varied between Neu1-KO and WT fish embryo. Expression of lysosomal-related genes, including ctsa, lamp1a, and tfeb were up-regulated in adult Neu1-KO muscle as compared with WT. Furthermore, the expression pattern of genes involved in bone remodeling (runx2a, runx2b, and mmp9) was decreased in Neu1-KO fish. These phenotypes were quite similar to those of Neu1-KO mice and human sialidosis patients, indicating the effectiveness of the established Neu1-KO zebrafish for the study of vertebrate Neu1 sialidase.

    DOI: 10.1042/BCJ20200348

    PubMed

  • Hideaki Matsui, Kazuhiro Shiozaki, Yutaka Okumura, Manabu Ishikawa, Viliame Waqalevu, Oki Hayasaka, Akinobu Honda, Tomonari Kotani .  Effects of phosphorous deficiency of a microalga Nannochloropsis oculata on its fatty acid profiles and intracellular structure and the effectiveness in rotifer nutrition .  ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS49   2020.8Effects of phosphorous deficiency of a microalga Nannochloropsis oculata on its fatty acid profiles and intracellular structure and the effectiveness in rotifer nutrition

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    DOI: 10.1016/j.algal.2020.101905

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  • Akinobu Honda, Petros Kingstone Chigwechokha, Ryo Takase, Oki Hayasaka, Koji Fujimura, Tomonari Kotani, Masaharu Komatsu, Kazuhiro Shiozaki .  Novel Nile tilapia Neu1 sialidases: Molecular cloning and biochemical characterization of the sialidases Neu1a and Neu1b .  GENE742   144538 - 144538   2020.6Novel Nile tilapia Neu1 sialidases: Molecular cloning and biochemical characterization of the sialidases Neu1a and Neu1bReviewed International journal

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    Lysosomal desialylation is the initial step in the degradation of sialo-glycopeptides that is essential for regenerating sialo-glycoconjugates. Neu1 sialidase is the enzyme responsible for the removal of sialic acid in the mammalian lysosome. Although Neu1 sialidases are conserved in fish similar to mammals, their physiological functions remain to be fully understood. Nile tilapia (Oreochromis niloticus) is known to possess two putative Neu1 sialidases (Neu1a and Neu1b) in the genome that may have arisen by gene duplication (specifically in cichlidae family members). This suggests that understanding the Neu1 sialidase in fish, particularly cichlids, could provide insights into the (novel) physiological functions of these genes. Moreover, characterization of the tilapia Neu1 sialidase is paramount to ensure clarity of the desialylation reaction performed by the fish sialidases (like the characterized tilapia sialidases Neu3 and Neu4). Therefore, this study focused on the characterization of the tilapia Neu1 sialidases. Neu1b exhibited narrow substrate specificity when compared with Neu1a, whereas the properties of these two Neu1 sialidases, such as cathepsin A-induced activation, optimal pH, and lysosomal localization, were conserved. Neu1a mRNA levels were detected in various tissues of tilapia as compared to the mRNA levels of Neu1b. Although the cloned construct of Neu1b contained an extra exon unlike tilapia Neu1a, the exon did not affect the enzymatic properties of Neu1b. This study suggests that tilapia Neu1a profiles were highly conserved with other vertebrate Neu1 isoforms, while Neu1b probably evolved independently in other members of the cichlidae family. Moreover, the expression of sialidase genes (neu1a, neu1b, neu3a, and neu4) were determined in various stages of tilapia embryogenesis using real-time PCR; sialidase gene expression is reported to be drastically and individually altered during embryogenesis in Japanese medaka (Oryzias latipes). The mRNA levels of neu1a drastically increased between 72 and 84 hpf and mildly decreased from 84 to 144 hpf. In contrast, the transcript levels of neu1b did not change between 84 and 144 hpf and the expression of neu3a gradually increased between 84 and 120 hpf and drastically decreased at 144 hpf. The highest level of the neu4 transcripts was detected at 84 hpf. These expression patterns were different from those in Japanese medaka, possibly due to the different developmental program found in the tilapia embryo accompanied with the unique profiles of the tilapia sialidases.

    DOI: 10.1016/j.gene.2020.144538

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  • Kazuhiro Shiozaki, Momoko Kawabe, Kiwako Karasuyama, Takayoshi Kurachi, Akito Hayashi, Koji Ataka, Haruki Iwai, Hinako Takeno, Oki Hayasaka, Tomonari Kotani, Masaharu Komatsu, Akio Inui .  Neuropeptide Y deficiency induces anxiety-like behaviours in zebrafish (Danio rerio) .  SCIENTIFIC REPORTS10 ( 1 ) 5913 - 5913   2020.4Neuropeptide Y deficiency induces anxiety-like behaviours in zebrafish (Danio rerio)Reviewed International journal

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    NeuropeptideY (NPY) controls energy homeostasis including orexigenic actions in mammalians and non-mammalians. Recently, NPY has attracted attention as a mediator of emotional behaviour and psychosomatic diseases. However, its functions are not fully understood. We established npy gene-deficient (NPY-KO) zebrafish (Danio rerio) to assess the relationship between NPY and emotional behaviours. The NPY-KO zebrafish exhibited similar growth, but pomc and avp mRNA levels in the brain were higher as compared to wild-type fish. NPY-KO zebrafish exhibited several anxiety-like behaviours, such as a decrease in social interaction in mirror test and decreased locomotion in black-white test. The acute cold stress-treated NPY-KO zebrafish exhibited anxiety-like behaviours such as remaining stationary and swimming along the side of the tank in the mirror test. Moreover, expression levels of anxiety-associated genes (orx and cck) and catecholamine production (gr, mr, th1 and th2) were significantly higher in NPY-KO zebrafish than in wild-type fish. We demonstrated that NPY-KO zebrafish have an anxiety phenotype and a stress-vulnerability like NPY-KO mice, whereby orx and/or catecholamine signalling may be involved in the mechanism actions.

    DOI: 10.1038/s41598-020-62699-0

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  • Kazuki Oishi, Mina Miyazaki, Ryo Takase, Petros Kingstone Chigwechokha, Masaharu Komatsu, Kazuhiro Shiozaki .  Regulation of triglyceride metabolism in medaka (Oryzias latipes) hepatocytes by Neu3a sialidase .  FISH PHYSIOLOGY AND BIOCHEMISTRY46 ( 2 ) 563 - 574   2020.4Regulation of triglyceride metabolism in medaka (Oryzias latipes) hepatocytes by Neu3a sialidaseReviewed International journal

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    Fish store triglycerides (TGs) in the liver, muscle, and adipose tissue and TGs constitute an energy source upon metabolic demand. The liver generally plays important roles in lipid metabolism. Recent studies have suggested the possibility of hepatic lipid metabolic regulation by ganglioside in mammals; however, ganglioside-mediated regulation of lipid metabolism is unclear in fish. This study aimed to clarify the role of ganglioside in fish TG metabolism, with particular reference to Neu3a, a ganglioside-specific sialidase expressed in the fish liver. Under fasting conditions, there was a decrease in hepatic TG contents, and neu3a mRNA level was significantly up-regulated in the medaka liver. To determine the role of Neu3a in hepatic lipid metabolism, Neu3a stable transfectants were generated using fish liver Hepa-T1 cells. After treating Neu3a cells with oleic acid, reduction of TG was detected in comparison with the mock cells. Furthermore, lipase activity was greater in Neu3a cells than in mock cells. To examine which ganglioside regulates these events, alterations of ganglioside composition in Neu3a cells were analyzed. Neu3a cells exhibited increased level of lactosylceramide (LacCer), a Neu3 enzymatic product originating from GM3. In addition, exposure of LacCer toward Hepa-T1 cells resulted in an increase of neutral lipase activity. The present results suggest that Neu3a up-regulation in medaka under fasting condition accelerates hepatic TG degradation for energy production via GM3 desialylation.

    DOI: 10.1007/s10695-019-00730-6

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  • Tran Nguyen Duy Khoa, Viliame Waqalevu, Akinobu Honda, Kazuhiro Shiozaki, Tomonari Kotani .  Comparative study on early digestive enzyme activity and expression in red sea bream (Pagrus major) fed on live feed and micro-diet .  AQUACULTURE519   2020.3Comparative study on early digestive enzyme activity and expression in red sea bream (Pagrus major) fed on live feed and micro-diet

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    The use of live feed to meet larval nutritional requirements has caused a bottleneck in marine fish larviculture whilst the use of micro-diets has had limited success in completely replacing live feeds. In this study, the variations in enzyme activity and its related gene expressions during early ontogenetic development of red sea bream (Pagrus major) were investigated. The experiment included four treatments: (i) using live feed (enriched L-type rotifers of the Brachionus plicatilis sp. complex) until 15 days after hatching (DAH) and gradually switching to Anemia; (ii) using micro-diet only; (iii) co-feeding diet (using both live feed and micro-diet) and, (iv) starvation (no food supplied). This study lasted 40 days and the activity and expression of digestive enzymes (trypsin (try), chymotrypsin (ctrb), pepsin (pep), amylase (amy2 alpha), pancreatic lipase (pl), expression of energy metabolism (ATP synthase (atp), cytochrome-c-oxidase (cox1), cholecystokinin (cck) and growth (insulin like growth factor 1 (igf1)) were linked to larval biometrics. After 40 DAH, no significant difference was observed in growth (igf1), energy metabolism (cox1, atp) and survival rate between live feed and co-feeding diet treatments. However, there were significantly higher differences observed when these were compared to the starvation and micro-diet only treatments. The pancreatic enzymes and cck were regulated by dietary compositions and detectable from hatching and fluctuated throughout larval ontogeny. While pep was initially observed only after 15 DAH. Limits of larval survival and low enzyme activity were observed when the larvae were subjected to starvation for 10 days and were able to adapt to micro-diet only after 15 DAH accompanied with a 5-day co-feeding transitionary period.

    DOI: 10.1016/j.aquaculture.2019.734721

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  • Oishi, K., Miyazaki, M., Takase, R., Chigwechokha, KP., Komatsu, M., Shiozaki, K .  Regulation of triglyceride metabolism in medaka (Oryzias latipes) hepatocytes by Neu3a sialidase. .  Fish Physiol. Biochem46   563 - 574   2020Regulation of triglyceride metabolism in medaka (Oryzias latipes) hepatocytes by Neu3a sialidase.Reviewed

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  • Matsui, H., Shiozaki, K., Okumura, Y., Ishikawa, M., Waqalevu, V., Hayasaka, V., Honda, A., Kotani, T. .  Effects of phosphorous deficiency of a microalga Nannochloropsis oculata on its fatty acid profiles and intracellular structure and the effectiveness in rotifer nutrition. .  Algal Res   2020Effects of phosphorous deficiency of a microalga Nannochloropsis oculata on its fatty acid profiles and intracellular structure and the effectiveness in rotifer nutrition.Reviewed

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  • Shiozaki, K, Kawabe, M., Karasuyama, K., Kurachi, T., Hayashi, A., Ataka, K., Iwai, H., Takeno, H., Hayasaka, O., Kotani, T., Komatsu, M., Inui, A .  Neuropeptide Y deficiency induces anxiety-like behaviours in zebrafish (Danio rerio). .  Sci. Rep10   NO:5913   2020 Neuropeptide Y deficiency induces anxiety-like behaviours in zebrafish (Danio rerio).

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  • Honda, A., Chigwechikha, PK., Takase, R., Hayasaka, O., Fujimura, K., Kotani, T., Komatsu, M., Shiozaki, K., .  Novel Nile tilapia Neu1 sialidases: Molecular cloning and biochemical characterization of the sialidases Neu1a and Neu1b .  Gene742   Article:144538   2020Novel Nile tilapia Neu1 sialidases: Molecular cloning and biochemical characterization of the sialidases Neu1a and Neu1bReviewed

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  • Okada, K., Takase, R., Hamaoka, Y., Honda, A., Ikeda, A., Hokazono, Y., Maeda, Y., Hayasaka, O., Kotani, T., Komatsu, M., Shiozaki, K .  Establishment and characterization of Neu1-knockout zebrafish and its abnormal clinical phenotypes .  Biochemical Journal477   2841 - 2857   2020Establishment and characterization of Neu1-knockout zebrafish and its abnormal clinical phenotypesReviewed

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  • Linh Khanh Vo, Toshiharu Tsuzuki, Yuko Kamada-Futagami, Petros Kingstone Chigwechokha, Akinobu Honda, Kazuki Oishi, Masaharu Komatsu, Kazuhiro Shiozaki .  Desialylation by Edwardsiella tarda is the initial step in the regulation of its invasiveness .  BIOCHEMICAL JOURNAL476 ( 21 ) 3183 - 3196   2019.11Reviewed International journal

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    Edwardsiella tarda is a gram-negative bacterium causing significant economic losses to aquaculture. E. tarda possesses NanA sialidase which removes sialic acids from alpha 2-3 sialo-glycoprotein of host cells. However, the relationship between NanA sialidase activity and E. tarda invasiveness remains poorly understood. Furthermore, the pathway of sialic acid metabolism in E. tarda remains to be elucidated. We studied sialidase activity in several E. tarda strains and found that the pathogenic strains exhibited higher sialidase activity and greater up-regulation of the NanA mRNA level than non-pathogenic strain. Pathogenic strains also showed higher rates of infection in GAKS cells, and the infection was drastically suppressed by sialidase inhibitor. Additionally, NanA gene overexpression significantly increased infection and treatment of E. tarda with free sialic acid enhanced the rate of infection in GAKS cells. Sialic acid treatment enhanced mRNA levels of two N-acetylneuraminate lyases and one N-acetylneuraminate cytidylyltransferase. E. tarda uses sialic acid as a carbon source for growth via N-acetylneuraminate lyases. The strains with high N-acetylneuraminate cytidylyltransferase level showed greater sialylation of the lipopolysaccharides and glycoproteins. Our study establishes the significance of desialylation by E. tarda sialidase in the regulation of its invasiveness.

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  • Megumi Matsumoto, Taisei Kubota, Sinsuke Fujita, Kazuhiro Shiozaki, Shosei Kishida, Atsushi Yamamoto .  Elucidation of the Interleukin 12 Production Mechanism during Intracellular Bacterial Infection in Amberjack, Seriola dumerili .  INFECTION AND IMMUNITY87 ( 11 )   2019.11Elucidation of the Interleukin 12 Production Mechanism during Intracellular Bacterial Infection in Amberjack, Seriola dumeriliReviewed International journal

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    Intracellular bacterial infections affect all vertebrates. Cultured fish are particularly vulnerable because no effective protection measures have been established since such infections emerged approximately 50 years ago. As in other vertebrates, the induction of cell-mediated immunity (CMI) plays an important role in protecting fish against infection. However, details of the mechanism of CMI induction in fish have not been clarified. In the present study, we focused on the production of interleukin 12 (IL-12), an important factor in CMI induction in fish. Using several different approaches, we investigated IL-12 regulation in amberjack (Seriola dumerili), the species most vulnerable to intracellular bacterial disease. The results of promoter assays and transcription factor gene expression analyses showed that the expression of interferon regulatory factor-1 (IRF-1) and activator protein-1 (AP-1) is necessary for IL-12 production. Phagocytosis of living cells (LCs) of Nocardia seriolae bacteria induced IL-12 production in neutrophils, accompanied by IRF-1 and AP-1 gene expression. Bacteria in which the exported repetitive protein (Erp)-like gene was deleted (Delta erp-L) could not establish intracellular parasitism or induce IRF-1 and AP-1 expression or IL-12 production, despite being phagocytosed by neutrophils. These data suggest that IL-12 production is regulated by (i) two transcription factors, IRF-1 and AP-1, (ii) phagocytosis of LCs by neutrophils, and (iii) one or more cell components of LCs. Our results enhance the understanding of the immune response to intracellular bacterial infections in vertebrates and could facilitate the discovery of new agents to prevent intracellular bacterial disease.

    DOI: 10.1128/IAI.00459-19

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  • Tran Nguyen Duy Khoa, Viliame Waqalevu, Akinobu Honda, Kazuhiro Shiozaki, Tomonari Kotani .  Early ontogenetic development, digestive enzymatic activity and gene expression in red sea bream (Pagrus major) .  AQUACULTURE512   2019.10Early ontogenetic development, digestive enzymatic activity and gene expression in red sea bream (Pagrus major)

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    Red sea bream (Pagrus major) is an important aquaculture species in Japan. This study aimed to determine the ontogenetic development of the digestive system, enzyme activity and related gene expressions of red sea bream larvae from hatching to post-metamorphosis (40 days after hatching, DAH). The larvae were fed with the euryhaline rotifer Brachionus plicatilis species complex L-type enriched with Chlorella vulgaris containing DHA from 3 to 15 DAH followed by Anemia nauplii from 16 to 40 DAH. Post culture analysis included, histological analysis of the larval gut, enzyme assays (trypsin, chymotrypsin, lipase, pepsin) and real time PCR with larvae sampled from 1 to 40 DAH. M hatching, the larval digestive tract was undifferentiated and resembled a straight tube. There was low detection of enzyme activity and gene expression of trypsin, chymotrypsin, lipase, and amylase during yolk-sac stage, before mouth opened. At 3 DAH, the mouth opened and the larvae began to exogenous feeding followed by the differentiation of the digestive tract into buccopharyngeal capacity and intestine at 5 DAH. The pancreas, liver, and gallbladder were functional indicating the transition from endogenous to exogenous feeding. The enzyme activity and gene expression of trypsin, chymotrypsin, lipase, and amylase increased and fluctuated throughout the larval development, a pattern that is common among marine fish species. Pepsin activity and gene expression were not detected until 15 DAH and strongly increased from 20 DAH. Metamorphosis was complete by 40 DAH following the regular arranging of microvilli and the abundance of vacuoles in intestine.

    DOI: 10.1016/j.aquaculture.2019.734283

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  • Viliame Waqalevu, Akinobu Honda, Serge Dossou, Tran Nguyen Duy Khoa, Hideaki Matsui, Kumbukani Mzengereza, Hanlin Liu, Manabu Ishikawa, Kazuhiro Shiozaki, Tomonari Kotani .  Effect of oil enrichment on Brachionus plicatilis rotifer and first feeding red sea bream (Pagrus major) and Japanese flounder (Paralichthys olivaceus) .  AQUACULTURE510   73 - 83   2019.8Effect of oil enrichment on Brachionus plicatilis rotifer and first feeding red sea bream (Pagrus major) and Japanese flounder (Paralichthys olivaceus)

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    Euryhaline rotifer belonging to the genera Brachionus are often used as live feed for first feeding marine finfish larviculture. Two common rotifer enrichments, a single usage of docosahexaenoic acid (DHA)-enriched Chlorella vulgaris and a combination of DHA-enriched C. vulgaris and salmon roe emulsion oil were used to assess: (i) nutritional status of rotifers and, (ii) performance when rotifers were fed to Pagrus major and Paralichthys olivaceus larvae from 2 to 15 days after hatching (DAH). Both rotifer enrichment treatments were found to be nutritionally effective with high unsaturated fatty acid (HUFA) content, especially in the combined treatment with significantly higher eicosapentaenoic acid (EPA). Despite deficient levels of EPA, DHA-enriched C. vulgaris was found to be a suitable enrichment for rotifer and also had positive response in survival, growth and trypsin activity in both P. major and P. olivaceus. DHA-enriched C. vulgaris only treatment had higher rotifer population growth (13% higher), egg bearing capacity (7% higher), almost double the soluble protein content and generally higher free amino acid content. Oxidation of lipids in the combined diet due to its high-saturated fatty acid content could be a possible cause to the inferior performance of the combined treatment. Both P. major (20.1 +/- 10.1%) and P. olivaceus (12.3 +/- 3.87%) suffered low survival and lower gut content in the combined treatment especially during periods of significant growth in both species (7-11 DAH). Despite high HUFA content in the combined treatment, this study revealed that consideration of free amino acids and soluble protein content as parameters of nutritional indicators for first feeding P. major and P. olivaceus should not be discounted.

    DOI: 10.1016/j.aquaculture.2019.05.039

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  • Oki Hayasaka, Yutaka Takeuchi, Kazuhiro Shiozaki, Kazuhiko Anraku, Tomonari Kotani .  Green light irradiation during sex differentiation induces female-to-male sex reversal in the medaka Oryzias latipes .  SCIENTIFIC REPORTS9 ( 1 ) 2383 - 2383   2019.2Green light irradiation during sex differentiation induces female-to-male sex reversal in the medaka Oryzias latipesReviewed International journal

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    This study investigated whether irradiation of a specific light wavelength could affect the sex differentiation of fish. We first found that the photoreceptor genes responsible for receiving red, green, and ultraviolet light were expressed in the eyes of medaka during the sex differentiation period. Second, we revealed that testes developed in 15.9% of genotypic females reared under green light irradiation. These female-to-male sex-reversed fish (i.e. neo-males) showed male-specific secondary sexual characteristics and produced motile sperm. Finally, progeny tests using the sperm of neo-males (XX) and eggs of normal females (XX) revealed that all F1 offspring were female, indicating for the first time in animals that irradiation with light of a specific wavelength can trigger sex reversal.

    DOI: 10.1038/s41598-019-38908-w

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  • Hayasaka, O., Takeuchi, Y., Shiozaki, K., Anraku, K., Kotani, T .  Green light irradiation during sex differentiation induces female-to-male sex reversal in the medaka Oryzias latipes .  Sci. Rep9   2383   2019Green light irradiation during sex differentiation induces female-to-male sex reversal in the medaka Oryzias latipesReviewed

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  • Tran, NDK., Waqalevu, V., Honda, A., Shiozaki, K., Kotani, T .  Comparative study on early digestive enzyme activity and expression in red sea bream (Pagrus major) fed on live feed and micro-diet. .  Aquaculture519   734721   2019Comparative study on early digestive enzyme activity and expression in red sea bream (Pagrus major) fed on live feed and micro-diet.Reviewed

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  • Vo, KL., Tsuzuki, T., Kamada-Futagami, Y., Chigwechokha KP., Honda, A., Oishi, K., Komatsu, M., Shiozaki, K .  Desialylation by Edwardsiella tarda is the initial step in the regulation of its invasiveness. .  Biochem. J476   3183 - 3196   2019Desialylation by Edwardsiella tarda is the initial step in the regulation of its invasiveness. Reviewed

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  • Matsumoto, M., Kubota, T., Fujita, S., Shiozaki, K., Kishida, S., Yamamoto., A .  Elucidation of the Interleukin 12 production mechanism during intracellular bacterial infection in amberjack, Seriola dumerili. .  Infect. Immun87   e00459-19,   2019Elucidation of the Interleukin 12 production mechanism during intracellular bacterial infection in amberjack, Seriola dumerili.Reviewed

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  • Tran, NDK., Waqalevu, V., Honda, A., Shiozaki, K., Kotani, T .  Early ontogenetic development, digestive enzymatic activity and gene expression in redseabream (Pagrus major) .  Aquaculture512   734283   2019Early ontogenetic development, digestive enzymatic activity and gene expression in redseabream (Pagrus major)Reviewed

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  • Waqalevu, V., Honda, A., Dossou, S., Tran, NDK., Matsui, H.,Mzengereza, K., Hanlin, L., Ishikawa, M., Shiozaki, K., Kotani, T .  Effect of oil enrichment on Brachionus plicatilis rotifer and first feeding red seabream (Pagrus major) and Japanese flounder (Paralichthys olivaceus) .  Aquaculture510   73 - 83   2019Effect of oil enrichment on Brachionus plicatilis rotifer and first feeding red seabream (Pagrus major) and Japanese flounder (Paralichthys olivaceus)Reviewed

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  • Waqalevu, VP., Matusi, H., Honda, A., Dossou, S., Yamamoto, A., Shiozaki, K., Kotani, T., .  Population growth, fatty acid composition and protein content of two types of Brachionus plicatilis sp. Complex rotifers fed four common Japanese nutritional enrichment diets. .  Aquacult. Sci67   139 - 156   2019Population growth, fatty acid composition and protein content of two types of Brachionus plicatilis sp. Complex rotifers fed four common Japanese nutritional enrichment diets.Reviewed

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  • Waqalevu Viliame, Matsui Hideaki, Honda Akinobu, Dossou Serge, Yamamoto Atsushi, Shiozaki Kazuhiro, Kotani Tomonari .  Enrichment effect on two <i>Brachionus plicatilis</i> sp. complex morphotypes fed three nutritional enrichment diets .  Aquaculture Science67 ( 2 ) 139 - 155   2019

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    <p>Information on effects of different enrichment on different morphotypes within the <i>Brachionus plicatilis</i> sp. complex is limited. This study investigated the effects of different enrichment diets on population growth, fatty acid content and protein content of L and SS types <i>B. plicatilis</i> sp. complex. Three commercial rotifer enrichment formulations were tested: docosahexaenoic acid (DHA) enriched <i>Chlorella vulgaris</i>, frozen <i>Nannochloropsis oculata</i>, salmon roe emulsion oil, against a control diet of normal <i>C. vulgaris</i>. DHA-enriched C. vulgaris and Frozen <i>N. oculata</i> had significantly higher population growth, fatty acid composition and soluble protein in both morphotypes. However, population growth under salmon roe emulsion treatment was affected negatively despite having high essential fatty acid content in both morphotypes. Variations in fatty acid assimilation in non-polar and polar lipids between morphotypes were observed highlighting enrichment effects and possible dissimilar biochemical roles of these compounds in tissue metabolism in each morphotype. Significantly higher levels of soluble protein were detected in DHA-enriched <i>C. vulgaris</i> and control treatment. SS-type had significantly higher soluble protein content except under the frozen <i>N. oculata</i> treatment. Culture method, enrichment type and dosage are important considerations as different rotifer morphotypes respond differently to different enrichment.</p>

    DOI: 10.11233/aquaculturesci.67.139

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    Other Link: https://agriknowledge.affrc.go.jp/RN/2010928154

  • Shiozaki Kazuhiro, Oishi Kazuki, Honda Akinobu .  Functional Characterization of Fish Sialidases and Their Diversity among Different Orders .  Trends in Glycoscience and Glycotechnology31 ( 178 ) J7 - J14   2019

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    Language:Japanese   Publisher:FCCA(Forum: Carbohydrates Coming of Age)  

    <p>Sialidase catalyzes the removal of sialic acids from glycoconjugates. While the physiological functions of mammalian sialidases have been elucidated, sialidases in other vertebrates, including those in fish, are not well understood. Recently, increased genomic resources in fish have become available, which will facilitate research on fish sialidase.</p><p>Here, three fish orders (Cypriniformes, Beloniformes and Perciformes) have been selected to compare the properties of their sialidases. A Neu1 knockdown in medaka by a morpholino oligo resulted in the accumulation of Sia α2-3 linked sialoglycoproteins, leading to malfunctions in heart beating, embryo growth, hatching, and swimming, indicating that lysosomal Neu1 is a crucial factor for embryogenesis.</p><p>Fish Neu3 and Neu4 function varies between fish species. Medaka and tilapia Neu3a localize at the plasma membrane, as is seen in humans. Zebrafish Neu3.1 is an ER sialidase, with an optimal pH (pH 2.6). Neu4 shows high diversity in subcellular localization: medaka and zebrafish Neu4 are detected at the lysosome and ER, respectively. Surprisingly, tilapia Neu4 is detected at nucleus, which makes it the first nuclear sialidase to be identified. Moreover, importin has been identified as a regulator of tilapia Neu4 functions. These results demonstrate the diversity of sialidase functions among fish species and the requirement of further fish research.</p>

    DOI: 10.4052/tigg.1518.1J

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  • Viliame Waqalevu, Akinobu Honda, Hideaki Matsui, Kazuhiro Shiozaki, Tomonari Kotani .  Proteolytic digestive enzyme response in Japanese flounder larvae Paralichthys olivaceus fed two types of rotifers Brachionus plicatilis species complex .  FISHERIES SCIENCE84 ( 6 ) 1037 - 1049   2018.11Proteolytic digestive enzyme response in Japanese flounder larvae Paralichthys olivaceus fed two types of rotifers Brachionus plicatilis species complex

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    Two common live feeds, the Brachionus plicatilis species complex SS-type and L-type were used to assess whether there were any differences in protein hydrolysis and digestive trypsin activity in first feeding Japanese flounder. There were no significant differences in hydrolysis activity at 2, 3 and 7 days after hatching (DAH). At 5 DAH, hydrolysis activity was significantly higher in larvae fed SS-type (p < 0.05) at 50 kDa in 1.5- and 3-h incubation whereas L-type treatment had not completely hydrolyzed the proteins after 3 h at the same molecular weight. Larvae fed SS-type had significantly higher (p < 0.05) trypsin activity at 3, 5, 6, 7 DAH. Contribution of live prey to trypsin fraction in larvae showed significantly higher (p < 0.05) fraction for SS-type at 5 DAH (2.18 +/- 0.44%) and 6 DAH (2.04 +/- 0.29%) and the effect of exogenous trypsin from live prey was relatively low when compared to the total trypsin activity in larvae. This study discusses the differences in ability to digest proteins in Japanese flounder when fed different rotifer morphotypes and highlights the adaptability of this species to alternative rotifer morphotypes during its early developmental stages.

    DOI: 10.1007/s12562-018-1241-2

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  • Megumi Matsumoto, Mahumoud Tanekey Amer, Kyosuke Araki, Atsushi Nishitani, Kazuma Hayashi, Yutaka Takeuchi, Kazuhiro Shiozaki, Atsushi Yamamoto .  Amberjack Seriola dumerili interleukin-10 negatively suppresses host cell-mediated immunity .  FISHERIES SCIENCE84 ( 5 ) 857 - 867   2018.9Amberjack Seriola dumerili interleukin-10 negatively suppresses host cell-mediated immunity

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    Interleukin-10 (IL-10) is an anti-inflammatory cytokine and negatively regulates cell-mediated immunity (CMI) induction by inhibiting cytokine production in type 1 T helper cells. IL-10 genes have been isolated from several fish, and inflammatory cytokine inhibition by IL-10 has been well examined. However, a CMI regulator of IL-10 in fish has not yet been identified. In this study, we cloned the IL-10 gene in amberjack Seriola dumerili and analyzed its function using its recombinant protein (rIL-10). In an in vitro culture experiment, gene expression of inflammatory cytokines was suppressed in leukocytes incubated with rIL-10 compared with cells that only received Nocardia seriolae stimulation. This result suggests amberjack IL-10 has conserved function as an inflammatory cytokine inhibitor. Bactericidal activity of amberjack cells against intracellular pathogen stimulation was decreased in a rIL-10 dose-dependent manner. Furthermore, a significant reduction in the T-bet/GATA-3 ratio was observed in N. seriolae living cell (LC) + rIL-10-injected fish. Taken together, these results suggest amberjack rIL-10 suppresses CMI induction both in vitro and in vivo. In addition, the number of IgM(+) cells among spleen leukocytes in N. seriolae + rIL-10-injected fish was higher than in only N. seriolae LC, suggesting that Th2-dominant immunity was induced by adding rIL-10.

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  • Megumi Matsumoto, Kyosuke Araki, Sayaka Nishimura, Hideki Kuriyama, Teruyuki Nakanishi, Kazuhiro Shiozaki, Yutaka Takeuchi, Atsushi Yamamoto .  Adjuvant effect in aquaculture fish of cell-wall glycolipids isolated from acid-fast bacteria .  DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY85   142 - 149   2018.8Adjuvant effect in aquaculture fish of cell-wall glycolipids isolated from acid-fast bacteria

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    Mycobacteriosis and nocardiosis in cultured fish caused by infections with acid-fast bacteria, are responsible for large economic losses globally. In this study, we suggest a novel adjuvant using glycolipids that activates host immune systems. The immune response to glycolipids stimulation was investigated using ginbuna crucian carp. Ginbuna vaccinated with FKC (formalin-killed cells) + glycolipids isolated from Mycobacterium sp., upregulated inflammatory- and Th1-related cytokines, and a DTH (delayed-type hypersensitivity) response was confirmed only in ginbuna vaccinated with FKC + glycolipids. These observations suggest that glycolipids activated host innate and cell-mediated immunity. Subsequently, we evaluated the adjuvant effect of glycolipids against amberjack nocardiosis. In a challenge test, a higher survival rate was observed in amberjack vaccinated with FKC + glycolipids emulsified with conventional oil adjuvant than in fish vaccinated with FKC + oil adjuvant without glycolipids. Therefore, glycolipids potentially could be used as a practical, economical and safe adjuvant for aquaculture fish. (C) 2018 Published by Elsevier Ltd.

    DOI: 10.1016/j.dci.2018.04.012

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  • Akinobu Honda, Petros Kingstone Chigwechokha, Yuko Kamada-Futagami, Masaharu Komatsu, Kazuhiro Shiozaki .  Unique nuclear localization of Nile tilapia (Oreochromis niloticus) Neu4 sialidase is regulated by nuclear transport receptor importin alpha/beta .  BIOCHIMIE149   92 - 104   2018.6Unique nuclear localization of Nile tilapia (Oreochromis niloticus) Neu4 sialidase is regulated by nuclear transport receptor importin alpha/beta

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    Sialidase catalyzes the removal of sialic acids from glycoconjugates. Different from Neu1 and Neu3 sialidases, Neu4 enzymatic properties such as substrate specificity and subcellular localization are not well-conserved among vertebrates. In fish only zebrafish and medaka neu4 genes have been cloned and their polypeptides have been characterized so far. Thus, characterization of Neu4 from other fish species is necessary to evaluate Neu4 physiological functions. Here, Nile tilapia was chosen for the characterization of Neu4 polypeptide considering that it is one of the major cultured fish all over the world and that its genomic sequences are now available. Coding DNA sequence of tilapia Neu4 was identified as 1,497 bp and its recombinant protein showed broad substrate specificity and optimal sialidase enzyme activity pH at 4.0. Neu4 activity was sustained even in neutral and alkali pH. Interestingly, immunofluorescence analysis revealed that major subcellular localization of tilapia Neu4 was nuclear, quite distinct from zebrafish (ER) and medaka Neu4 (lysosome). Bioinformatic analysis showed the existence of putative nuclear localization signal (NLS) in tilapia Neu4. In general, it is known that importin families bind to several proteins via NLS and transfer them into nucleus. Therefore, to determine the involvement of putative NLS in Neu4 nuclear localization, Neu4 mutant deleting NLS was constructed and expressed in cultured cells. As a result, NLS deletion significantly diminished the nuclear localization. Furthermore, treatment of importazole, interrupter of binding importin beta and RanGTP, significantly suppressed Neu4 nuclear localization. In summary, tilapia Neu4 is a unique sialidase localized at nucleus and its transport system into nucleus is regulated by importin. (C) 2018 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.

    DOI: 10.1016/j.biochi.2018.04.003

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  • Miyagi, T., Takahashi, K., Yamamoto, K., Shiozaki, K., Yamaguchi, K .  Biological and Pathological Roles of Ganglioside Sialidases. .  Prog. Mol. Biol. Transl. Sci156   121 - 150   2018Biological and Pathological Roles of Ganglioside Sialidases.Reviewed

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  • Waqalevu, VP., Honda, A., Matsui, H., Shiozaki, K., Kotani, T .  Proteolytic digestive enzyme response in Japanese flounder larve Paralichthys olivaceus red two types of rotifers Brachionus plicatilis species complex. .  Fish Sci84   1037 - 1049   2018Proteolytic digestive enzyme response in Japanese flounder larve Paralichthys olivaceus red two types of rotifers Brachionus plicatilis species complex.Reviewed

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  • Matsumoto, M., Amer, MT., Araki, K., Nishitani, A., Hayashi, K., Takeuchi, Y., Shiozaki, K., Yamamoto, A .  Amberjack Seriola dumerili interleukin-10 negatively suppresses host cell-mediated immunity .  Fish Sci84   857 - 867   2018Amberjack Seriola dumerili interleukin-10 negatively suppresses host cell-mediated immunityReviewed

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  • Matsumoto, M., Araki, K., Nishimura, S., Kuriyama, H., Nakanishi, T., Shiozaki, K., Takeuchi, Y., Yamamoto, A. .  Adjuvant effect in aquaculture fish of cell-wall glycolipids isolated from acid-fast bacteria. .  Dev. Comp. Immunol.85   142 - 149   2018Adjuvant effect in aquaculture fish of cell-wall glycolipids isolated from acid-fast bacteria.Reviewed

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  • . Honda, A., Chigwechokha, P., Kamada-Futagami, Y., Komatsu, M., Shiozaki, K .  Unique nuclear localization of Nile tilapia (Oreochromis niloticus) Neu4 sialidase is regulated by nuclear transport receptor importin alpha/beta. .  Biochimie142   94 - 104   2018Unique nuclear localization of Nile tilapia (Oreochromis niloticus) Neu4 sialidase is regulated by nuclear transport receptor importin alpha/beta.Reviewed

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  • Taeko Miyagi, Kohta Takahashi, Koji Yamamoto, Kazuhiro Shiozaki, Kazunori Yamaguchi .  Biological and Pathological Roles of Ganglioside Sialidases .  GANGLIOSIDES IN HEALTH AND DISEASE156   121 - 150   2018Biological and Pathological Roles of Ganglioside Sialidases

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    Sialidases are glycosidases responsible for the removal of alpha-glycosidically linked sialic acid residues from carbohydrate portions of glycoproteins and glycolipids, this process being the initial step in the degradation of such glycoconjugates. Sialic acids are considered to play important roles in various biological processes largely in two ways, one related to their hydrophilic and acidic properties exerting physicochemical effects on the glycoconjugates to which they are attached, and the other as recognition sites or in an opposing fashion as masking sites. The removal of sialic acids catalyzed by a sialidase, therefore greatly influences many biological processes through changing the conformation of glycoproteins and through recognition and masking of biological sites of functional molecules. Sialidases are found widely distributed in metazoan animals, from echinoderms to mammals, and are also present in viruses and other microorganisms, including fungi, protozoa, and bacteria even mostly lacking sialic acids. In mammals, there are four forms of sialidase (Neu1, Neu2, Neu3, and Neu4), differing in their major subcellular localization and enzymatic properties. They have been implicated in regulation of various cellular activities, such as cell differentiation, cell growth, and cell adhesion and motility, depending on their particular properties. In contrast, in microorganisms the enzymes appear to play roles limited to nutrition and pathogenesis. In this chapter, the focus is on mammalian sialidases preferentially hydrolyzing gangliosides, mostly Neu3 and Neu4, with an attempt to provide a brief overview of their physiological and pathological roles.

    DOI: 10.1016/bs.pmbts.2017.12.005

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  • Matsumoto, M., Araki, K., Hayashi, K., Takeuchi, Y., Shiozaki, K., Suetake, H., Yamamoto, A. .  Adjuvant effect of recombinant interleukin-12 in the Nocardiosis formalin-killed vaccine of the amberjack Seriola dumerili .  Fish Shellfish Immunol67   263 - 269   2017.8Adjuvant effect of recombinant interleukin-12 in the Nocardiosis formalin-killed vaccine of the amberjack Seriola dumerili

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  • Megumi Matsumoto, Kyosuke Araki, Kazuma Hayashi, Yutaka Takeuchi, Kazuhiro Shiozaki, Hiroaki Suetake, Atsushi Yamamoto .  Adjuvant effect of recombinant interleukin-12 in the Nocardiosis formalin-killed vaccine of the amberjack Seriola dumerili .  FISH & SHELLFISH IMMUNOLOGY67   263 - 269   2017.8Adjuvant effect of recombinant interleukin-12 in the Nocardiosis formalin-killed vaccine of the amberjack Seriola dumerili

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    Nocardiosis causes serious economic damage in the fish farming of Japanese yellowtail fish. Nocardia seriolae identified as pathogenic bacterium is an intracellular-pathogen. In general, induction of cell mediated immunity (CMI) is effective in infection defense against intracellular-pathogen. However, the conventional formalin-killed N. seriolae (FKC) vaccine induces humoral immunity. Interleukin-12 (IL-12) is Th1 type heterodimeric cytokine and induces cell differentiation in mammals. Our previous study showed that recombinant amberjack IL-12 has a role in CMI induction in vitro and could be a possible CMI inducing adjuvant. However, its adjuvant effect of fish IL-12 was not studied. In the present study, six types of amberjack recombinant IL-12 (rIL-12) were mixed and injected into amberjack with FKC. Firstly, we analyzed Th1- and Th2-related gene expression and monitored Th1/Th2 status followed by investigation of antibody titer. As a result, Th1-type immunity was induced in FKC + rIL-12 vaccinated fish. Secondly, we checked Th1/Th2 status of vaccinated fish after 10 days of N. seriolae infection using the expression of related genes. High T-bet/GATA-3 ratio was observed in FKC + rIL-12 vaccinated fish, suggesting that Thl cells possesing antigen memory were induced against N. seriolae infection. Finally, the survival rate in challenge test showed that 88% of FKC + rIL-12 vaccinated fish was survived at 34 days after N. seriolae injection whereas PBS (control) and FKC only were exterminated. These result suggest that i) rIL-12 is viable CMI inducible adjuvant and ii) production of Thl cells having antigen memory resulting from activation of IL-12 signaling pathway is important for defense against N. seriolae infection. (C) 2017 Published by Elsevier Ltd.

    DOI: 10.1016/j.fsi.2017.06.025

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  • akumi, S., Shimono, T., Ikema, S., Hotta, Y., Chigwechokha, P., Shiozaki, K., Sugiyama, Y., Hashimoto, M., Furukawa T., Komatsu, M .  Overexpression of Carboxylesterase Contributes to the Attenuation of Cyanotoxin Microcystin-LR .  oxicity. Comp. Biochem. Physiol. C Toxicol. Pharmacol194   22 - 27   2017.4Overexpression of Carboxylesterase Contributes to the Attenuation of Cyanotoxin Microcystin-LR Reviewed

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  • Shota Takumi, Tai Shimono, Satoshi Ikema, Yuki Hotta, Petros K. Chigwechokha, Kazuhiro Shiozaki, Yasumasa Sugiyama, Mitsuru Hashimoto, Tatsuhiko Furukawa, Masaharu Komatsu .  Overexpression of carboxylesterase contributes to the attenuation of cyanotoxin microcystin-LR toxicity .  COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY194   22 - 27   2017.4Overexpression of carboxylesterase contributes to the attenuation of cyanotoxin microcystin-LR toxicity

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    Microcystin-LR is a hepatotoxin produced by several cyanobacteria. Its toxicity is mainly due to a inhibition of protein phosphatase, PP1 and PP2A. Previously, we used a cell line stably expressing uptake transporter for microcystin-LR, OATPIB3 (HEK293-OATP1B3 cells). In this study, to determine whether overexpression of carboxylesterase (CES), which degrades ester-group and amide-group, attenuates the cytotoxicity of microcystin-LR, we generated the HEK293-OATPIB3/CES2 double-transfected cells. HEK293-OATP1B3/CES2 cells showed high hydrolysis activity of p-nitrophenyl acetate (PNPA), which is an authentic substrate for esterase. CES activity in HEK293-OATP1B3/CES2 cells was approximately 3-fold higher than that in the HEK293-OATP1B3 cells. HEK293-0ATP1B3/CES2 cells (IC50: 25.4 +/- 7.7 nM) showed approximately 2.1-fold resistance to microcystin-LR than HEK293-OATP1B3 cells (IC50: 12.0 +/- 1.5 nM). Moreover, the CES inhibition assay and microcystin-agarose pull down assay showed the possibility of the interaction between CES2 and microcystin-LR. Our results indicated that the overexpression of CES2 attenuates the cytotoxicity of microcystin-LR via interaction with microcystin-LR. (C) 2017 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cbpc.2017.01.008

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  • Sena Ryuzono, Ryo Takase, Yuko Kamada, Takanori Ikenaga, Petros Kingstone Chigwechokha, Masaharu Komatsu, Kazuhiro Shiozaki .  Suppression of Neu1 sialidase delays the absorption of yolk sac in medaka (Oryzias latipes) accompanied with the accumulation of alpha 2-3 sialo-glycoproteins .  BIOCHIMIE135   63 - 71   2017.4Suppression of Neu1 sialidase delays the absorption of yolk sac in medaka (Oryzias latipes) accompanied with the accumulation of alpha 2-3 sialo-glycoproteins

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    Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neul physiological functions in fish are still unclear. Here, to understand Neu1 significance in medaka embryogenesis, sialidase translation knockdown was carried out with one-cell. stage fertilized egg using morpholino oligo injection.Neul exhibited desialylation of alpha 2-3 sialic acid linkage in vitro and lysosomal localization in medaka caudal fin primary cells. Chloroquine treatment, inhibitor of lysosomal enzymes, caused an accumulation of alpha 2-3 sialo-glycoproteins in the primary cells. During the embryogenesis neu1 mRNA level was elevated until 3.5 day post fertilization (dpf) while an initial decrease of alpha 2-3 sialo-glycoprotein was observed around the same developmental stage. Neu1 knockdown by morpholino oligo induced some abnormal phenotypes such as delay of yolk sac absorption and small embryos. Sialidase-knockdown embryos also showed increase of heart rate in 5.5 and 6.5 dpf. Furthermore, about 37% decrease of hatching rate was observed in Neu1-MO treated embryos compared with control MO. Embryos showing severe phenotypes stopped embryogenesis at the late stage of development. Alteration of embryonic sialo-glycoproteins induced by morpholino injection was examined by lectin blotting to clarify the mechanism of abnormal development. As a result, degradation of several alpha 2-3 sialo-glycoproteins was suppressed in Neu1-MO embryo, possibly induced by the interruption of lysosomal desialylation toward yolk glycoprotein. Our results suggest that medaka Neu1 could be crucial for embryonic development through the degradation of yolk sac nutrition. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.

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  • Kazuhiro Shiozaki, Minori Yoshikawa, Saori Kiguchiya, Asami Ikeda, Yuko Kamada, Petros Kingstone Chigwechokha, Masaharu Komatsu .  Matrix metalloproteinase-7 inhibitory activity of lipid extract from dwarf gulper shark (Centrophorus atromarginatus) through down-regulation of gene transcription .  JOURNAL OF FUNCTIONAL FOODS30   90 - 96   2017.3Matrix metalloproteinase-7 inhibitory activity of lipid extract from dwarf gulper shark (Centrophorus atromarginatus) through down-regulation of gene transcription

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    Deep-sea living dwarf gulper shark (Centrophorus atromarginatus) is industrially important resource with lots of squalene in the liver, used as the resource for cosmetic ingredients, while consumption of shark muscle is limited because of ammonia smell caused by bacterial urease. Here, effect of shark muscle lipid on matrix metalloproteinase-7 (MMP-7) activity was estimated to develop the new application of shark muscle. Treatment of shark lipid toward HT-29 cells attenuated MMP-7 activity in conditioned medium accompanied with its decreased extracellular secretion. Ganglioside types in shark lipid were investigated by thin layer chromatography. Among shark gangliosides, GDIa and GM1 attenuated MMP-7 activity. GDla negatively regulated MMP-7 polypeptide and its gene expression in HT-29 cells, whereas MAPKs signaling pathways were not altered. GDIa suppressed c-jun expression in HT-29 cells, which could negatively regulate mmp-7 transcription. This study indicated the possibility of dwarf gulper shark muscle as a material of functional food. (C) 2017 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jff.2017.01.013

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  • Sayaka Shinyoshi, Yuko Kamada, Koki Matsusaki, Petros Kingstone Chigwechokha, Supawan Tepparin, Kyosuke Araki, Masaharu Komatsu, Kazuhiro Shiozaki .  Naringenin suppresses Edwardsiella tarda infection in GAKS cells by NanA sialidase inhibition .  FISH & SHELLFISH IMMUNOLOGY61   86 - 92   2017.2Naringenin suppresses Edwardsiella tarda infection in GAKS cells by NanA sialidase inhibitionInvited Reviewed

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    Edwardsiella tarda (E. tarda) is a gram-negative bacterium, which causes Edwardsiellosis in aquaculture. Previous studies indicate that E. tarda NanA sialidase plays crucial roles in infection through the desialylation of glycoproteins in fish cells. On the other hand, 2-deoxy-2,3-dehydro-N-acetylneuraminic acid, classic sialidase inhibitor, negatively regulates E. tarda infection of goldfish scale GAKS cells. Here, to development the suppression model of E. tarda infection for aquaculture application, the possibility of NanA inhibitory activities in citrus phytochemicals was evaluated as citrus extracts have widely been used as a supplement in fish diets for the improvement of meat quality. Some flavanones such as naringenin, hesperetin, hesperidin and naringin showed sialidase inhibitory activity toward recombinant NanA in vitro. Among them, naringenin showed the most potent inhibitory activity and its inhibitory pattern was non-competitive. Naringenin significantly suppressed E. tarda infection in GAKS cells at 200 and 400 mu M without bactericidal effect on E. tarda. On the other hand, naringin, glycosylation form of naringenin, showed slight suppression of E. tarda infection toward GAKS cells, suggesting the glycosides on flavanone could be important for NanA inhibition. Fluorescence microscopy analysis verified that number of invading E. tarda in GAKS cells was declined by naringenin treatment. The present study exhibited the possibility of naringenin as an effective ingredient in fish diet for the inhibition of E. tarda infection. (C) 2016 Elsevier Ltd. All rights reserved.

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  • Shiozaki, K., Yoshikawa, M., Kiguchiya, S., Ikeda, A., Kamada, Y., Chigwechokha, P., Komatsu, M .  Matrix metalloproteinase-7 inhibitory activity of lipid extract from dwarf gulper shark (Centrophorus atromarginatus) through down-regulation of gene transcription. .  J. Funct. Food30   90 - 96   2017.1Matrix metalloproteinase-7 inhibitory activity of lipid extract from dwarf gulper shark (Centrophorus atromarginatus) through down-regulation of gene transcription.Reviewed

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  • Ryuzono, S., Takase, R., Kamada, Y., Ikenaga, T., Chigwechokha, P., Komatsu, M., Shiozaki. K .  Suppression of Neu1 sialidase delays the absorption of yolk sac in medaka (Oryzias latipes) accompanied with the accumulation of alpha 2-3 sialo-glycoproteins .  Biochimie   2017.1Suppression of Neu1 sialidase delays the absorption of yolk sac in medaka (Oryzias latipes) accompanied with the accumulation of alpha 2-3 sialo-glycoproteinsReviewed

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  • Yoshinaga, A., Kajiya, M., Oishi, K., Kamada, Y., Ikeda, A., Chigwechokha, P., Kibe, T., Kishida, M., Kishida, S., Komatsu, M., Shiozaki, K .  NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulation .  European Journal of Pharmacology782   21 - 29   2016.4NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulationReviewed

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  • Kanouchi. H., Kakimoto. T., Nakano. H., Nakai. Y., Shiozaki. K., Akikoka. K., Otomaru. K., Matsumoto. M., Nagano. M .  The brewed rice vinegar Kurozu increases HSPA1A expression and ameliorates cognitive dysfunction in aged P8 mice .  PLOS ONE4   e0150769   2016.3The brewed rice vinegar Kurozu increases HSPA1A expression and ameliorates cognitive dysfunction in aged P8 miceReviewed

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  • 10.1016/j.biochi.2016.01.010 .    123   65 - 72   2016.110.1016/j.biochi.2016.01.010 Reviewed

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  • Funahashi, A., Komatsu, M., Furukawa, T., Yoshizono, Y., Yoshizono, H., Orikawa, Y., Takumi, S., Shiozaki, K., Hayashi, S., Kaminishi, Y., Itakura, T .  Eel green fluorescent protein is associated with resistance to oxidative stress .  Comp. Biochem. Physiol. C181-182   35 - 39   2015.12Eel green fluorescent protein is associated with resistance to oxidative stressReviewed

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  • Ryuzono, S., Takase, R., Oishi, K., Ikeda, A., Chigwechokha, P., Funahashi, A., Komatsu, M., Miyagi, T., Shiozaki, K .  Lysosomal localization of Japanese medaka (Oryzias latipes) Neu1 sialidase and its highly conserved enzymatic profiles with human. .  Gene575   513 - 523   2015.10Lysosomal localization of Japanese medaka (Oryzias latipes) Neu1 sialidase and its highly conserved enzymatic profiles with human.Reviewed

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  • Chigwechokha, P., Tabata, M., Shinyoshi, S., Oishi, K., Araki, K., Komatsu, M., Itakura, T., Shiozaki, K .  Recombinant sialidase NanA (rNanA) cleaves alpha 2-3-linked sialic acid of host cell surface N-linked glycoprotein to promote Edwardsiella tarda infection .  Fish and Shellfish Immunology47   34 - 45   2015.8Recombinant sialidase NanA (rNanA) cleaves alpha 2-3-linked sialic acid of host cell surface N-linked glycoprotein to promote Edwardsiella tarda infectionReviewed

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  • Ikema, S., Takumi, S., Maeda, Y., Kurimoto, T., Bohda, S., Chigwechokha, P., Sugiyama, Y., Shiozaki, K., Furukawa, T., Komatsu M., .  Okadaic acid is taken-up into the cells mediated by human hepatocytes transporter OATP1B3 .  Food Chem Toxicol83   229 - 236   2015.6Okadaic acid is taken-up into the cells mediated by human hepatocytes transporter OATP1B3Reviewed

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  • Asami Ikeda, Hayato Ichino, Saori Kiguchiya, Petros Chigwechokha, Masaharu Komatsu, Kazuhiro Shiozaki .  Evaluation and identification of potent angiotensin-I converting enzyme inhibitory peptide derived from dwarf gulper shark (Centrophorus atromarginatus). .  J Food Proc Pres39   107   2015.4 Evaluation and identification of potent angiotensin-I converting enzyme inhibitory peptide derived from dwarf gulper shark (Centrophorus atromarginatus). Reviewed

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  • Koji Yamamoto, Kohta Takahashi, Kazuhiro Shiozaki, Kazunori Yamaguchi, Setsuko Moriya, Masahiro Hosono, Hiroshi Shima, Taeko Miyagi .  Potentiation of epidermal growth factor -mediated oncogenic transformation by sialidase NEU3 leading to Src activation. .  PloS ONE10   e0120578   2015.3Potentiation of epidermal growth factor -mediated oncogenic transformation by sialidase NEU3 leading to Src activation.Reviewed

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  • Shota Takumi, Satoshi Ikema, Tamami Hanyu, Yusuke Shima, Takashi Kurimoto, Kazuhiro Shiozaki, Yasumasa Sugiyama, Ho-Dong Park, Seiichi Ando, Tatsuhiko Furukawa, Masaharu Komatsu .  Naringin attenuates the cytotoxicty of hepatotoxin microcystin-LR by the curious mechanisms to OATP1B1- and OATP1B3-expressing cells .  Environ Toxicol Pharmacol39   974 - 981   2015.3Naringin attenuates the cytotoxicty of hepatotoxin microcystin-LR by the curious mechanisms to OATP1B1- and OATP1B3-expressing cellsReviewed

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  • Kazuhiro Shiozaki, Kohta Takahashi, Masahiro Hosono, Kazunori Yamaguchi, Keiko Hata, Momo Shiozaki, Rosaria Bassi, Alessandro Prinetti, Sandro Sonnino, Kazuo Nitta and Taeko Miyagi .  Phosphatidic acid-mediated activation and translocation to the cell surface of sialidase NEU3, promoting signaling for cell migration .  FASEB Jin press   2015.2Phosphatidic acid-mediated activation and translocation to the cell surface of sialidase NEU3, promoting signaling for cell migration Reviewed

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  • Keiko Hata, Tatsuo Tochigi, Ikuro Sato, Sadafumi Kawamura, Kazuhiro Shiozaki, Tadashi Wada, Kohta Takahashi, Setsuko Moriya, Kazunori Yamaguchi, Masahiro Hosono and Taeko Miyagi .  Increased sialidase activity in serum of cancer patients: identification of sialidase and inhibitor activities in human serum .  Cancer Sciin press   2015.1Increased sialidase activity in serum of cancer patients: identification of sialidase and inhibitor activities in human serumReviewed

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  • Petros Chigwechokha, Masaharu Komatsu, Takao Itakura, Kazuhiro Shiozaki .  Nile Tilapia Neu3 sialidases: molecular cloning, functional characterization and expression in Oreochromis niloticus .  Gene552   155 - 164   2014.11Nile Tilapia Neu3 sialidases: molecular cloning, functional characterization and expression in Oreochromis niloticusReviewed

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  • Hiroyuki Takano, Shota Takumi, Satoshi Ikema, Nozomi Mizoue, Yuki Hotta, Kazuhiro Shiozaki, Yasumasa Sugiyama, Tasuhiko Furukawa, Masaharu Komatsu .  Microcystin-LR Induces Anoikis Resistance to the Hepatocyte Uptake Transporter OATP1B3-expressing Cell Lines .  Toxicology326   53 - 61   2014.10Microcystin-LR Induces Anoikis Resistance to the Hepatocyte Uptake Transporter OATP1B3-expressing Cell LinesReviewed

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  • Kazuhiro Shiozaki, Sena Ryuzono, Naoto Matsushita, Asami Ikeda, Kazuki Takeshita, Petros Chigwechokha, Masaharu Komatsu, Taeko Miyagi .  Molecular cloning and biochemical characterization of medaka (Oryzias latipes) lysosomal neu4 sialidase .  Fish Physiol Biochem40   1461 - 1472   2014.4Molecular cloning and biochemical characterization of medaka (Oryzias latipes) lysosomal neu4 sialidase Reviewed

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  • Masaharu Komatsu, Naoki Ichiyama, Takashi Kurimoto, Shota Takumi, Kazuhiro Shiozaki, Yasumasa Sugiyama, Tatsuhiko Furukawa, Seiichi Ando, Saki Itonori, Hiroaki Saito .  Ceramide aminoethylphosphonate from jumbo flying squid Dosidicus gigas attenuates the toxicity of cyanotoxin microcystin-LR .  Fish Sci79   313 - 320   2013.3Ceramide aminoethylphosphonate from jumbo flying squid Dosidicus gigas attenuates the toxicity of cyanotoxin microcystin-LRReviewed

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  • Kazuhiro Shiozaki, Kazuki Takeshita, Mako Ikeda, Asami Ikeda, Yusuke Harasaki, Masaharu Komatsu, Shoji Yamada, Kazunori Yamaguchi, Taeko Miyagi .  Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes) .  Biochimie95   280 - 289   2013.2Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes)Reviewed

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  • Kazunori Yamaguchi, Kazuhiro Shiozaki, Setsuko Moriya, Koichi Koseki, Tadashi Wada, Hiroo Tateno, Ikuro Sato, Masahide Asano, Yoichiro Iwakura, Taeko Miyagi .  Reduced susceptibility to colitis-associated colon carcinogenesis in mice lacking plasma membrane-associated sialidase .  PLoS One7 ( 7 ) e41132   2012.7Reduced susceptibility to colitis-associated colon carcinogenesis in mice lacking plasma membrane-associated sialidaseReviewed

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  • Kohta Takahashi, Junya Mitoma, Masahiro Hosono, Kazuhiro Shiozaki, Chihiro Sato, Kazunori Yamaguchi, Ken Kitajima, Hideyoshi Higashi, Kazuo Nitta, Hiroshi Shima, Taeko Miyagi1 .  Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neurons .  J Biol Chem287   14816 - 14826   2012.3Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neuronsReviewed

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  • Takahiro Oku, Seiichi Ando, Hsin-Chun Tsai, Yusuke Yamashita, Hiroshi Ueno, Kazuhiro Shiozaki, Ryuichiro Nishi, Shoji Yamada .  Purification and identification of two carnosine-cleaving enzymes,carnosine dipeptidase I and Xaa-methyl-His dipeptidase, from Japanese eel(Anguilla japonica) .  Biochimie94   1281 - 1290   2012.2Purification and identification of two carnosine-cleaving enzymes,carnosine dipeptidase I and Xaa-methyl-His dipeptidase, from Japanese eel(Anguilla japonica)Reviewed

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  • Takahiro Oku, Seiichi Ando, Takehiko Hayakawa, Kyoko Baba, Ryuichiro Nishi,Kazuhiro Shiozaki, Shoji Yamada .  Purification and identification of a novel primitive secretory enzyme catalyzingthe hydrolysis of imidazole-related dipeptides in the jawless vertebrateLethenteron reissneri .  Peptides32   648 - 655   2011.6Purification and identification of a novel primitive secretory enzyme catalyzingthe hydrolysis of imidazole-related dipeptides in the jawless vertebrateLethenteron reissneriReviewed

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  • Kazuhiro Shiozaki, Kazunori Yamaguchi, Kohta Takahashi, Setsuko Moriya, Taeko Miyagi .  Regulation of sialyl lewis antigen expression in colon cancer cells by sialidase NEU4 .  J Biol Chem286 ( 24 ) 21052 - 21061   2011.6Regulation of sialyl lewis antigen expression in colon cancer cells by sialidase NEU4Reviewed

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  • Kazuhiro Shiozaki, Momo Shiozaki, Junko Masuda, Akiko Yamauchi, Shuichi Ohwada, Toshiki Nakano, Toshiyasu Yamaguchi, Tadao Saito, Koji Muramoto, Minoru Sato .  Identification of oyster-derived hypotensive peptide acting as angiotensin I converting enzyme inhibitor .  Fish Sci76 ( 5 ) 865 - 872   2010.6Identification of oyster-derived hypotensive peptide acting as angiotensin I converting enzyme inhibitorReviewed

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  • Kazuhiro Shiozaki, Koichi Koseki, Kazunori Yamaguchi, Momo Shiozaki, Hisashi Narimatsu, Taeko Miyagi .  Developmental change of sialidase Neu4 expression in murine brain and its involvement in the regulation of neuronal cell differentiation. .  J Biol Chem284   21157 - 21164   2009.6Developmental change of sialidase Neu4 expression in murine brain and its involvement in the regulation of neuronal cell differentiation.Reviewed

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  • Kazuhiro Shiozaki, Kazunori Yamaguchi, Ikuro Sato, Taeko Miyagi .  Plasma membrane-associated sialidase (NEU3) promotes formation of colonic aberrant crypt foci in azoxymethane-treated transgenic mice. .  Cancer Sci100   588 - 594   2009.2Plasma membrane-associated sialidase (NEU3) promotes formation of colonic aberrant crypt foci in azoxymethane-treated transgenic mice.Reviewed

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  • Takuji Uemura, Kazuhiro Shiozaki, Kazunori Yamaguchi, Kaoru Miyazaki, Susumu Satomi, Kengo Kato, Taeko Miyagi .  Contribution of sialidase NEU1 to suppression of metastasis of human colon cancer cells through desialylation of integrin beta 4. .  Oncogene28   1218 - 1229   2009.1Contribution of sialidase NEU1 to suppression of metastasis of human colon cancer cells through desialylation of integrin beta 4.Reviewed

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  • Taeko Miyagi, Tadashi Wada, Kazunori Yamaguchi, Kazuhiro Shiozaki, Ikuro Sato, Yoichiro Kakugawa, Hideaki Yamanami, Tsuneaki Fujiya .  Human sialidase as a cancer marker .  Proteomics8   3303 - 3311   2008.7Human sialidase as a cancer markerReviewed

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  • Taeko Miyagi, Tadashi Wada, Kazunori Yamaguchi, Keiko Hata, Kazuhiro Shiozaki .  Plasma membrane- associated sialidase as a crucial regulator of transmembrane signaling. .  J Biochem144   279 - 285   2008.6Plasma membrane- associated sialidase as a crucial regulator of transmembrane signaling.Reviewed

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  • Kazuhiro Shiozaki, Hideaki Yananami, Kazunori Yamaguchi, Tadashi Wada, Taeko Miyagi .  Down-regulation of Sialidase NEU4 in colon cancer and its involvement in expression of malignant properties .  Glycoconjugate J24   381   2007.7Down-regulation of Sialidase NEU4 in colon cancer and its involvement in expression of malignant propertiesReviewed

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  • Tadashi Wada, Keiko Hata, Kazunori Yamaguchi, Kazuhiro Shiozaki, Koichi Koseki, Setsuko Moriya, Taeko Miyagi .  A crucial role of plasma membrane-associated sialidase in the survival of human cancer cells .  Oncogene26   2483 - 2490   2007.3A crucial role of plasma membrane-associated sialidase in the survival of human cancer cellsReviewed

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  • Hideaki Yamanami, Kazuhiro Shiozaki, Tadashi Wada, Kazunori Yamaguchi, Takuji Uemura, Yoichiro Kakugawa, Tsuneaki Fujiya, Taeko Miyagi .  Down-regulation of sialidase NEU4 may contribute to invasive properties of human colon cancers .  Cancer Sci98   299 - 307   2007.3Down-regulation of sialidase NEU4 may contribute to invasive properties of human colon cancersReviewed

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  • Kazuhiro Shiozaki, Koichi Koseki, Kazunori Yamaguchi, Momo Shiozaki, Taeko Miyagi .  Developmental change of Neu4 expression in mouse brain and its involvement in neuronal cell differentiation .  Trends Glycosci Glycotech18   S43   2006.8Developmental change of Neu4 expression in mouse brain and its involvement in neuronal cell differentiationReviewed

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  • Minoru Sato, Zhihua Tao, Kazuhiro Shiozaki, Toshiki Nakano, Toshiyasu Yamaguchi, Takehiko Yokoyama, Nobuhiro Kanno, Eizou Nagahisa .  A simple and rapid method for the analysis of fish histamine by paper electrophoresis .  Fish Sci72   889 - 892   2006.7A simple and rapid method for the analysis of fish histamine by paper electrophoresisReviewed

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  • Kazuhiro Shiozaki, Akiko Fujii, Toshiki Nakano, Toshiyasu Yamaguchi, Minoru Sato .  Inhibitory effects of hot water extract of the stevia stem on the contractile response of the smooth muscle of the guinea pig ileum. .  Biosci Biotechnol Biochem70   489 - 494   2006.2Inhibitory effects of hot water extract of the stevia stem on the contractile response of the smooth muscle of the guinea pig ileum.Reviewed

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  • Kazunori Yamaguchi, Keiko Hata, Koichi Koseki, Kazuhiro Shiozaki, Hirotoshi Akita, Tadashi Wada, Setsuko Moriya, Taeko Miyagi .  Evidence for mitochondrial localization of a novel human sialidase (NEU4) .  Biochem J390   85 - 93   2005.4Evidence for mitochondrial localization of a novel human sialidase (NEU4)Reviewed

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  • Kazuhiro Shiozaki, Toshiki Nakano, Toshiyasu Yamaguchi, Minoru Sato, Naohiko Sato .  The protective effect of stevia extract on the gastric mucosa of rainbow trout Oncorhynchus mykiss (Walbaum) fed dietary histamine. .  Aquacult Res35   1421 - 1428   2004.12The protective effect of stevia extract on the gastric mucosa of rainbow trout Oncorhynchus mykiss (Walbaum) fed dietary histamine.Reviewed

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  • Kazuhiro Shiozaki, Toshiki Nakano, Toshiyasu Yamagushi, Minoru Sato .  Metabolism of exogenous histamine in rainbow trout (Oncorhynchus mykiss) .  Fish Physiol Biochem29   289 - 295   2003.7Metabolism of exogenous histamine in rainbow trout (Oncorhynchus mykiss)Reviewed

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  • Minoru Sato, Zhihua Tao, Kazuhiro Shiozaki, Toshiki Nakano, Toshiyasu Yamaguchi, Tsutomu Kumagai, Takehiko Yokoyama, Nobuhiro Kanno, Eizou Nagahisa .  A rapid method for histamine analysis of seafoods by paper electrophoresis .  ITE lett Batt New technol Med3   68 - 70   2002.7A rapid method for histamine analysis of seafoods by paper electrophoresisReviewed

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Books

  • Mammalian Sialidase and Tumor Development

    Taeko Miyagi, Kohta Takahashi, Kazuhiro Shiozaki, Kazunori Yamaguchi( Role: Joint author)

    Sugar Chains  2015.1 

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    Language:English Book type:Scholarly book

  • Mammalian Sialidase Assays

    Taeko Miyagi, Kohta Takahashi, Kazuhiro Shiozaki, Kazunori Yamaguchi( Role: Joint author)

    Glycoscience:Biology and Medicine  2014.5 

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    Language:English Book type:Scholarly book

MISC

  • 魚類シアリダーゼの生理機能とその魚種間における多様性 Reviewed

    塩崎一弘, 大石一樹, 本田晃伸

    Trend. Glycosci. Glycotech.   31   j7 - j14   2020

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 令和元年度日本水産学会九州支部大会,例会および若手交流会

    大富 潤, 小谷 知也, 石川 学, 塩﨑 一弘

    日本水産学会誌   86 ( 3 )   227 - 229   2020

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    Publisher:公益社団法人 日本水産学会  

    DOI: 10.2331/suisan.WA2718

    CiNii Books

  • Functional characterization of fish sialidases and their diversity among different orders. Reviewed

    Trend. Glycosci. Glycotech

    Trend. Glycosci. Glycotech   31   E7 - E13   2019

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  • Development of preventive method for Edwardsiellosis : Significance of sialoglycoconjugates in Edwardsiella tarda infection

    ( 29 )   33 - 40   2019

  • Enrichment effect on two <i>Brachionus plicatilis</i> sp. complex morphotypes fed three nutritional enrichment diets

    Waqalevu Viliame, Matsui Hideaki, Honda Akinobu, Dossou Serge, Yamamoto Atsushi, Shiozaki Kazuhiro, Kotani Tomonari

    Aquaculture Science   67 ( 2 )   139 - 155   2019

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    Publisher:Japanese Society for Aquaculture Science  

    <p>Information on effects of different enrichment on different morphotypes within the <i>Brachionus plicatilis</i> sp. complex is limited. This study investigated the effects of different enrichment diets on population growth, fatty acid content and protein content of L and SS types <i>B. plicatilis</i> sp. complex. Three commercial rotifer enrichment formulations were tested: docosahexaenoic acid (DHA) enriched <i>Chlorella vulgaris</i>, frozen <i>Nannochloropsis oculata</i>, salmon roe emulsion oil, against a control diet of normal <i>C. vulgaris</i>. DHA-enriched C. vulgaris and Frozen <i>N. oculata</i> had significantly higher population growth, fatty acid composition and soluble protein in both morphotypes. However, population growth under salmon roe emulsion treatment was affected negatively despite having high essential fatty acid content in both morphotypes. Variations in fatty acid assimilation in non-polar and polar lipids between morphotypes were observed highlighting enrichment effects and possible dissimilar biochemical roles of these compounds in tissue metabolism in each morphotype. Significantly higher levels of soluble protein were detected in DHA-enriched <i>C. vulgaris</i> and control treatment. SS-type had significantly higher soluble protein content except under the frozen <i>N. oculata</i> treatment. Culture method, enrichment type and dosage are important considerations as different rotifer morphotypes respond differently to different enrichment.</p>

    DOI: 10.11233/aquaculturesci.67.139

    CiNii Books

    Other Link: https://agriknowledge.affrc.go.jp/RN/2010928154

  • NodularinはOATP1B3発現細胞に対して上皮間葉転換様の形質転換を誘導する

    富岡優, 内匠正太, 杉山靖正, 塩崎一弘, 古川龍彦, 小松正治

    日本生化学会大会(Web)   90th   ROMBUNNO.1P‐0567 (WEB ONLY)   2017

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  • Plasma membrane-associated sialidase confers cancer initiation, promotion and progression

    Taeko Miyagi, Kohta Takahashi, Kazuhiro Shiozaki, Kazunori Yamaguchi, M Hosono

    Adv Exp Med Biol   842   139 - 145   2014.12

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  • シアリダーゼによる細胞接着の制御

    高橋耕太、塩崎一弘、宮城妙子

    生体の科学   64 ( 3 )   273 - 278   2013.6

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  • Sialidase significance for cancer progression

    Taeko Miyagi, Kohta Takahashi, Keiko Hata, Kazuhiro Shiozaki, Kazunori Yamaguchi

    Glycoconj J   29   567 - 577   2012.12

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  • Altered Expression of Sialidases in Human Cancer

    Taeko Miyagi, Kohta Takahashi, Setsuko Moriya, Keiko Hata, Kazunori Yamamoto, Tadashi Wada, Kazunori Yamaguchi, Kazuhiro Shiozaki

    Adv Exp Med Biol   749   257 - 267   2012.1

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  • 癌の浸潤・転移と糖鎖

    宮城妙子、上村卓嗣、 塩崎一弘、 山口壹範

    実験医学   25   1042 - 1048   2007.5

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Presentations

  • 高瀬 諒、本田晃伸、小松正治、塩﨑一弘   脊椎動物モデルへの応用を目指した魚類オートファジー・リソソーム分解系における脱シアリル化機構の解明  

    第39回日本分子生物学会年会 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Poster presentation  

  • 大石一樹・宮崎未奈・小松正治・塩﨑一弘   肥満の発症におよぼすガングリオシド脱シアリル化の意義  

    39回日本分子生物学会年会 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Poster presentation  

  • 堀田 夕貴、内匠 正太、橋本 海理、塩崎 一弘、池田 龍二、古川 龍彦、小松 正治   マイクロシスチンLRが誘導するアノイキス抵抗性細胞の低酸素応答  

    第39回日本分子生物学会年会 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Poster presentation  

  • 橋本 海理、青木 俊二、池田 龍二、堀田 夕貴、塩崎 一弘、古川 龍彦、内匠 正太、小松 正治   アクテオシドを用いたマイクロシスチンLRおよびファロイジンの細胞毒性の抑制  

    第39回日本分子生物学会年会 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Poster presentation  

  • 塩崎一弘   柑橘が持つがん・感染症予防機能の検証と鹿児島での展開   Invited

    第六回食と健康に関するシンポジウム 健康長寿をかなえる 鹿児島の食と健康 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  • 本田晃伸、Petros Chigwechokha、鎌田裕子、小松正治、塩崎一弘   ティラピアNeu4は核に局在するシアル酸水解酵素である  

    平成28年度日本水産学会秋季大会, 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 塩崎一弘、龍薗せな、高瀬 諒、鎌田裕子、大石一樹、宮崎未奈、小松正治、池永隆徳   胚発生におけるリソソームシアリダーゼNeu1、Neu4の重要性  

    平成28年度日本水産学会秋季大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 塩崎一弘、新吉沙弥香、松崎光輝、鎌田裕子、荒木亨介、小松正治   柑橘由来フラボノイドのEdwardsiella tarda感染抑制効果  

    平成28年度日本水産学会秋季大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 山本晃司、高橋耕太、塩崎一弘、山口和範、島礼、宮城妙子   形質膜シアリダーゼNEU3のEGFR活性化機構  

    第89回日本生化学会大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (keynote)  

  • 松本 萌、西村沙也香、早志和真、塩崎一弘、中西照幸、山本 淳、荒木亨介   魚類ミコバクテリア症原因菌が合成する糖脂質の細胞性免疫誘導型アジュバント としての有効性  

     平成28年度魚病学会秋季大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • Kanouchi, H., Suzuki, M., Kakimoto, T., Nakano, H., Nakai, Y., Shiozaki, K., Nagano, M., Matsumoto. M   Kurozu, brewed rice vinegar, induce heat shock protein 70 and ameliorates congnitive dysfunction in aged SAM P8 mice   International conference

    Food for Health International Conference (FOHIC2016) 

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    Event date: 2016.3

    Language:English   Presentation type:Poster presentation  

  • Yoshinaga, A., Oishi, K., Kamada, Y., Komatsu, M., Shiozaki, K   Naringin suppresses cancer cell growth through the up-regulation of GM3 ganglioside   International conference

    Food for Health International Conference (FOHIC2016) 

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    Event date: 2016.3

    Language:English   Presentation type:Poster presentation  

  • 塩崎 一弘   シアル酸分解阻害を介したカンキツ由来フラバノンの機能性   Invited

    第2回鹿児島の黒膳 機能性食と健康シンポジウム 

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    Event date: 2016.1

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 新吉沙弥香、若松麗菜、Petros Kingstone Chigwechokha、松崎光輝、荒木亨介、小松正治、塩崎一弘   E.tardaの感染予防を目的としたシアリダーゼNanA阻害物質の探索  

    平成27年度日本水産学会九州支部会 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Poster presentation  

  • 吉永綾奈、大石一樹、小松正治、塩崎一弘   シアリダーゼNEU3を標的としたフラボノイドの新規機能性  

    平成27年度日本水産学会九州支部会 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Poster presentation  

  • 高瀬 諒、龍薗せな、若松麗菜、Petros Kingstone Chigwechokha、本田晃伸、荒木亨介、小松正治、塩崎一弘   魚類肝臓におけるシアリダーゼNeu1の意義  

    平成27年度日本水産学会九州支部会 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Poster presentation  

  • 龍薗せな、高瀬 諒、大石一樹、小松正治、塩崎一弘   魚類オートファジーにおける糖タンパク質分解の活性化機構とその重要性  

    平成27年度日本水産学会九州支部会 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Poster presentation  

  • Kanouchi, H., Suzuki, M., Kakimoto, T., Nakano, H., Nakai, Y., Shiozaki, K., Nagano, M., Matsumoto. M   Kurozu, brewed rice vinegar, feeding ameliorates cognitive dysfunction in aged SAM P8 mice   International conference

    The 6th International Conference on Food Factors – ICoF 

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    Event date: 2015.11

    Language:English   Presentation type:Oral presentation (general)  

  • 大石一樹、小松正治、塩崎一弘   魚類ガングリオシドシアリダーゼNeu3aの形質膜局在機構  

    平成27年度日本水産学会秋季大会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 龍薗せな、高瀬諒、大石一樹、池田麻美、舟橋亜希、小松正治、塩崎一弘   魚類リソソームにおける脱シアリル化機構の解明とその意義  

    平成27年度日本水産学会秋季大会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 塩崎一弘、Petros Chigwechokha、新吉沙弥香、大木麗菜、荒木亨介、小松正治   Edwardsiella tarda感染における宿主細胞のN型糖タンパク糖鎖構造変化の重要性  

    平成27年度日本水産学会秋季大会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 塩崎 一弘   水棲生物を利用した健康機能性研究   Invited

    第3回日本統合医療学会鹿児島支部学術大会 

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    Event date: 2015.8

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  • Petros Chigwechokha、新吉沙弥香、本田晃伸、大木麗菜、荒木亨介、小松正治、宮城妙子、塩崎一弘   Edwardsiella tarda感染による脱シアリル化機構とその意義  

    第34回日本糖質学会年会 

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    Event date: 2015.7

    Language:English   Presentation type:Poster presentation  

  • 池間 智、内匠正太、Petros Chigwechokha、杉山靖正、塩崎一弘、古川龍彦、小松正治   下痢性解読オカダ酸はOATP1B1およびOATP1B3の輸送基質として細胞毒性を発現する   International conference

    第8回トランスポーター研究会 

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    Event date: 2015.7

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 池間 智、内匠正太、塩崎一弘、杉山靖正、古川龍彦、小松正治   ナリンジンは肝臓毒マイクロシスチンLRのOATP1B1およびOATP1B3を介した毒性発現を様々なメカニズムで減弱する  

    第8回トランスポーター研究会 

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    Event date: 2015.7

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 橋本海理、池間 智、内匠正太、青木俊二、池田龍二、杉山靖正、塩崎一弘、古川龍彦、小松正治   生薬を用いたマイクロシスチンLRおよびオカダ酸中毒の発現抑制   International conference

    第8回トランスポーター研究会 

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    Event date: 2015.7

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 堀田夕貴、高野博之、池間 智、内匠正太、杉山靖正、塩崎一弘、古川龍彦、小松正治   肝臓毒マイクロシスチンLRはOATP1B3発現細胞にアノイキス抵抗性を誘導する  

    第8回トランスポーター研究会 

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    Event date: 2015.7

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 塩﨑一弘、龍薗せな、大石一樹、高瀬諒、吉永綾奈、小松正治、宮城妙子   ニホンメダカにおける脱シアリル化機構の解明  

    第34回日本糖質学会年会 

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    Event date: 2015.7

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 龍薗せな、高瀬諒、大石一樹、小松正治、宮城妙子、塩崎一弘   メダカリソソームシアリダーゼの胚発生における意義  

    第34回日本糖質学会年会 

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    Event date: 2015.7

    Language:Japanese   Presentation type:Poster presentation  

  • Kakimoto, K., Nakano, H., Nakai, Y., Shiozaki, K., Akikoka, K., Otomaru, K., Nakamura, T., Matsumoto, M., Nagano, M., Sugimoto, Y., Kanouchi, H   Kurozu increases HSPA1A expression and ameliorates cognitive dysfunction in aged SAM P8 mice   International conference

    The 29th Annual Symposium of The Protein Soc 

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    Event date: 2015.7

    Language:English   Presentation type:Oral presentation (general)  

  • 塩崎 一弘   鹿児島県特産品を利用した機能性食品の開発   Invited

    機能性食と健康シンポジウム 

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    Event date: 2015.1

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 塩崎 一弘   鹿児島県近海の未利用資源をターゲットにした機能性研究  

    第19回フードファクター学会  第19回フードファクター学会

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    Event date: 2014.11

    Language:Japanese  

    Venue:鹿児島  

    国内学会

  • 吉永綾奈、原崎裕介、小松正治、塩崎一弘   がん糖脂質を標的にしたカンキツ類の細胞増殖抑制効果  

    第19回フードファクター学会  第19回フードファクター学会

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    Event date: 2014.11

    Language:Japanese  

    Venue:鹿児島  

    国内学会

  • Chigwechokha, P., Itakura T., Komatsu, M., Shiozaki, K   Molecular cloning and characterization of gaglioside specific sialidase in tilapia  

    平成26年度日本水産学会秋季大会  平成26年度日本水産学会秋季大会

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    Event date: 2014.9

    Language:Japanese  

    Venue:福岡  

    国内学会

  • 原崎裕介、池田麻美、龍薗せな、吉川みのり、大石一樹、小松正治、塩崎一弘   筋分化におけるメダカシアリダーゼNeu3bの機能解析  

    平成26年度日本水産学会秋季大会  平成26年度日本水産学会秋季大会

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    Event date: 2014.9

    Language:Japanese  

    Venue:福岡  

    国内学会

  • 池間 智、橋本海里、堀田夕貴、杉山靖正、塩崎一弘、内匠正太、小松正治   生薬ウコンによるマイクロシスチンLRおよびオカダ酸の毒性抑制  

    平成26年度日本水産学会秋季大会  平成26年度日本水産学会秋季大会

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    Event date: 2014.9

    Language:Japanese  

    Venue:福岡  

    国内学会

  • 龍薗せな、池田麻美、原崎裕介、大石一樹、小松正治、宮城妙子、塩崎一弘   ニホンメダカOryzias latipesのシアリダーゼ研究におけるモデル動物としての有用性  

    第33回日本糖質学会年会  第33回日本糖質学会年会

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    Event date: 2014.8

    Language:Japanese  

    Venue:愛知  

    国内学会

  • 塩崎一弘、龍薗せな、池田麻美、原崎裕介、福田みどり、小松正治、宮城妙子   メダカシアリダーゼneu4の遺伝子クローニングおよび胚発生における発現解析  

    第32回日本糖質学会年会  第32回日本糖質学会年会

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    Event date: 2013.8

    Language:Japanese  

    Venue:大阪  

    国内学会

  • 宮城妙子、高橋耕太、細野雅祐、山本晃司、和田 正、秦 敬子、森谷節子、塩崎一弘、山口壹範、仁田一雄   シアリダーゼNEU3によるがん化能の制御  

    第32回日本糖質学会年会  第32回日本糖質学会年会

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    Event date: 2013.8

    Language:Japanese  

    Venue:大阪  

    国内学会

  • 塩崎一弘   鹿児島近海に生息するサメ類の有効利用法の検討  

    研究コアプロジェクト「島嶼」、「環境」、「食と健康」 ~その現状と将来への展望~  研究コアプロジェクト「島嶼」、「環境」、「食と健康」 ~その現状と将来への展望~

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    Event date: 2013.3

    Language:Japanese  

    Venue:鹿児島  

    研究会

  • 塩崎一弘、竹下一輝、池田麻美、原崎裕介、小松正治、山田章二、宮城妙子   メダカシアリダーゼneu3a、neu3bの性状解析とその生理的役割の検討  

    第31回日本糖質学会年会  第31回日本糖質学会年会

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    Event date: 2012.9

    Language:Japanese  

    Venue:鹿児島  

    国内学会

  • 前川継徳、池田麻美、安部洋平、加治屋夏希、小松正治、山田章二、塩崎一弘   サメ筋肉に由来する健康機能性ペプチドの探索  

    平成24年度日本水産学会秋季大会  平成24年度日本水産学会秋季大会

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    Event date: 2012.9

    Language:Japanese  

    Venue:山口  

    国内学会

  • 堂免康人、森山史、高野博之、直江昌宏、塩崎一弘、山田章二、杉山靖正、林征一、小松正治   魚類血清を用いた代替細胞培養法の検討  

    平成24年度日本水産学会秋季大会  平成24年度日本水産学会秋季大会

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    Event date: 2012.9

    Language:Japanese  

    Venue:山口  

    国内学会

  • 秦 敬子, 森谷節子, 細野雅祐, 和田 正, 塩崎一弘, 仁田一雄, 宮城妙子   ヒト担がんにおけるシアリダーゼ活性の同定  

    第31回日本糖質学会年会  第31回日本糖質学会年会

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    Event date: 2012.9

    Language:Japanese  

    Venue:鹿児島  

    国内学会

  • 竹下一輝、池田真子、池田麻美、小松正治、山田章二、塩崎一弘   新規メダカシアリダーゼolNeu3a、olNeu3bのクローニングと性状解析  

    平成23年度日本水産学会九州支部会大会  平成23年度日本水産学会九州支部会大会

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    Event date: 2012.1

    Language:Japanese  

    Venue:鹿児島  

    国内学会

  • 塩崎一弘、前川継徳、市野隼人、中村好成,、波江野皆樹、小松正治、山田章二   鹿児島近海に生息するサメ類由来の健康機能性ペプチド探索  

    平成23年度日本水産学会九州支部大会  平成23年度日本水産学会九州支部大会

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    Event date: 2012.1

    Language:Japanese  

    Venue:鹿児島  

    国内学会

  • 高橋耕太、三苫純也、塩崎一弘、山口壹範、細野雅佑、佐藤ちひろ、北島健、東秀好、仁田一雄、宮城妙子   シアリダーゼNeu4はポリシアル酸分解を触媒し、海馬ニューロンの伸長を抑制する  

    第84回日本生化学会大会  第84回日本生化学会大会

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    Event date: 2011.9

    Language:Japanese  

    Venue:京都  

    国内学会

  • 塩崎一弘、竹下一輝、池田真子、小松正治、山田章二、山口壹範、宮城妙子   メダカシアリダーゼNeu3のクローニングおよび性状解析  

    第84回日本生化学会大会  第84回日本生化学会大会

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    Event date: 2011.9

    Language:Japanese  

    Venue:京都  

    国内学会

  • 秦敬子、和田正、横井則貴、川崎ナナ、塩崎一弘、山口壹範、高橋耕太、森谷節子、細野雅佑、仁田一雄、宮城妙子   形質膜シアリダーゼNEU3の膜トポロジーに関する研究  

    第84回日本生化学会大会  第84回日本生化学会大会

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    Event date: 2011.9

    Language:Japanese  

    Venue:京都  

    国内学会

  • 宮城妙子, 和田 正, 山口壹範, 秦 敬子, 森谷節子, 塩崎一弘, 高橋耕太, 山本晃司   がんで過剰発現する形質膜シアリダーゼNEU3 の悪性化進展機構  

    第30回日本糖質学会年会  第30回日本糖質学会年会

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    Event date: 2011.7

    Language:Japanese  

    Venue:新潟  

    国内学会

  • 塩崎一弘   未利用資源の利用を目指した機能性食品の開発  

    ミニシンポジウム「与論島における水産食品資源活用ならびに藻場造成」  ミニシンポジウム「与論島における水産食品資源活用ならびに藻場造成」

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    Event date: 2011.3

    Language:Japanese  

    Venue:鹿児島  

    研究会

  • 秦敬子、和田正、山口壹範、高橋耕太、森谷節子、塩崎一弘、宮城妙子   シアリダーゼNEU3によるEGFRシグナリング活性化機構の解析  

    第83回日本生化学会大会  第83回日本生化学会大会

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    Event date: 2010.12

    Language:Japanese  

    Venue:兵庫  

    国内学会

  • 高橋耕太、塩崎一弘、山口壹範、森谷節子、宮城妙子   シアリダーゼNeu4による神経接着分子NCAMポリシアル酸分解の制御  

    第83回日本生化学会大会  第83回日本生化学会大会

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    Event date: 2010.12

    Language:Japanese  

    Venue:兵庫  

    国内学会

  • 塩崎一弘、前川継徳、佐藤實   水産物由来の降圧ペプチドの開発と今後の展開  

    平成22年度日本水産学会九州支部会  平成22年度日本水産学会九州支部会

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    Event date: 2010.10

    Language:Japanese  

    Venue:鹿児島  

    国内学会

  • Kazuhiro Shiozaki, Kazunori Yamaguchi, Kohta Takahashi, Taeko Miyagi   Sialidase NEU4 attenuates E-selectin derived signaling in colon cancer cells   International conference

    Sialoglyco meeting.  Sialoglyco meeting.

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    Event date: 2010.8

    Language:English  

    Venue:ドイツ  

    国際学会

  • 塩崎一弘、山口壹範、宮城妙子   シアリダーゼNEU3はフォスファチジン酸により活性化される  

    生理学研究所研究会 糖鎖機能研究会  生理学研究所研究会 糖鎖機能研究会

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    Event date: 2010.7

    Language:Japanese  

    Venue:岡崎  

    研究会

  • Kazuhiro Shiozaki, Keiko Hata, Kazunori Yamaguchi, Taeko Miyagi   Activation of plasma membrane-associated sialidase NEU3 by phospholipid   International conference

    The 28th Naito Conference on Glycan Expression and Regulation [I] Functions and Disease Mechanisms  The 28th Naito Conference on Glycan Expression and Regulation [I] Functions and Disease Mechanisms

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    Event date: 2010.7

    Language:English  

    Venue:神奈川  

    国際学会

  • 山口壹範、塩崎一弘、森谷節子、秦 敬子、和田 正、宮城妙子   形質膜シアリダーゼノックアウトマウスにおける大腸がん発症の抑制  

    第3回東北糖鎖研究会  第3回東北糖鎖研究会

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    Event date: 2009.11

    Language:Japanese  

    Venue:新潟  

    研究会

  • 秦敬子、 塩崎桃、 山口壹範、 塩崎一弘、 和田正、 森谷節子、 宮城妙子   形質膜シアリダーゼによるEGF レセプターシグナリング活性化の分子機構  

    第82回日本生化学会大会  第82回日本生化学会大会

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    Event date: 2009.10

    Language:Japanese  

    Venue:兵庫  

    国内学会

  • 塩崎一弘、秦敬子、和田正、塩崎桃、山口壹範、宮城妙子   NEU3とリン脂質の相互作用による細胞運動亢進の分子機構  

    第82回日本生化学会大会  第82回日本生化学会大会

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    Event date: 2009.10

    Language:Japanese  

    Venue:兵庫  

    国内学会

  • 高橋耕太、山口壹範、塩崎一弘、森谷節子、宮城妙子   シアリダーゼによるCD44 の機能制御  

    第82回日本生化学会大会  第82回日本生化学会大会

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    Event date: 2009.10

    Language:Japanese  

    Venue:兵庫  

    国内学会

  • 塩崎一弘、山口壹範、和田正、秦敬子、森谷節子、宮城妙子   神経細胞の分化におけるシアリダーゼの役割  

    第29回日本糖質学会年会  第29回日本糖質学会年会

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    Event date: 2009.9

    Language:Japanese  

    Venue:岐阜  

    国内学会

  • 塩崎一弘、秦敬子、山口壹範、宮城妙子   リン脂質によるシアリダーゼの活性化機構  

    第75回日本生化学会東北支部会  第75回日本生化学会東北支部会

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    Event date: 2009.5

    Language:Japanese  

    Venue:宮城  

    国内学会

  • Momo Shiozaki, Kazuhiro Shiozaki, Setsuko Moriya, Kazunori Yamaguchi, Taeko Miyagi   NEU3 possibly interacts with EGFR at cytoplasmic side of plasma membrane  

    第81回日本生化学会大会  第81回日本生化学会大会

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    Event date: 2008.12

    Language:Japanese  

    Venue:兵庫  

    国内学会

  • Kazuhiro Shiozaki, Keiko Hata, Momo Shiozaki, Kazunori Yamaguchi, Taeko Miyagi   A possible mechanism of NEU3 activation at plasma membrane  

    第81回日本生化学会大会  第81回日本生化学会大会

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    Event date: 2008.12

    Language:Japanese  

    Venue:兵庫  

    国内学会

  • 塩崎一弘、山口壹範、宮城妙子   Activation of Met receptor-derived signaling by plasma membrane-associated sialidase (NEU3)  

    第67回日本癌学会学術総会  第67回日本癌学会学術総会

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    Event date: 2008.10

    Language:Japanese  

    Venue:愛知  

    国内学会

  • 塩崎一弘、上村卓嗣、山口壹範、宮城妙子   シアリダーゼNEU1によるがん細胞の浸潤・転移抑制機構  

    第28回日本糖質学会年会  第28回日本糖質学会年会

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    Event date: 2008.8

    Language:Japanese  

    Venue:茨城  

    国内学会

  • Kazuhiro Shiozaki, Kazunori Yamaguchi, Ikuro Sato, Taeko Miyagi   Increased incidence of aberrant crypt foci (ACF) in mice overexpressing plasma membrane-associated sialidase NEU3  

    第80回日本生化学会大会  第80回日本生化学会大会

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    Event date: 2007.12

    Language:Japanese  

    Venue:神奈川  

    国内学会

  • 山口壹範、塩崎一弘、小関弘恵知、宮城妙子   がんで高発現するシアリダーゼNEU3の発現調節機構の解析  

    第1回東北糖鎖研究会  第1回東北糖鎖研究会

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    Event date: 2007.12

    Language:Japanese  

    Venue:宮城  

    研究会

  • Momo Shiozaki, Kazunori Yamaguchi, Keiko Hata, Kazuhiro Shiozaki, Taeko Miyagi   Plasma membrane-asociated sialidase NEU3 interacts with EGF receptor in specific domains  

    第80回日本生化学会大会  第80回日本生化学会大会

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    Event date: 2007.12

    Language:Japanese  

    Venue:神奈川  

    国内学会

  • Kazuhiro Shiozaki, Hidaki Yamanami, Tadashi Wada, Kazunori Yamaguchi, Taeko Miyagi   Down-regulation of sialidase NEU4 in colon cancer and its involvement of malignant phenotypes   International conference

    XIX International Symposium on Glycoconjugates  XIX International Symposium on Glycoconjugates

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    Event date: 2007.7

    Language:English  

    Venue:オーストラリア  

    国際学会

  • 塩崎一弘、小関弘恵知、山口壹範、宮城妙子   シアリダーゼNeu4は神経細胞の分化を抑制する  

    第72回日本生化学会東北支部会  第72回日本生化学会東北支部会

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    Event date: 2007.5

    Language:Japanese  

    Venue:宮城  

    国内学会

  • 塩崎一弘、山並秀章、山口壹範、宮城妙子   Sialidase NEU4は大腸がんの悪性形質を制御する  

    生理学研究所研究会 病態糖鎖研究会  生理学研究所研究会 病態糖鎖研究会

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    Event date: 2006.9

    Language:Japanese  

    Venue:愛知  

    研究会

  • Kazuhiro Shiozaki, Hideaki Yamanami, Takuji Uemura, Tadashi Wada, Kazunori Yamaguchi, Taeko Miyagi   Novel sialidase NEU4 suppresses cell motility of colon cancer cells through attenuation of E-selectin-derived signaling   International conference

    The 11th International Congress of Metastasis Research Society Jointed with the 15th Annual Meeting of Japanese Association for Metastasis Research  The 11th International Congress of Metastasis Research Society Jointed with the 15th Annual Meeting of Japanese Association for Metastasis Research

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    Event date: 2006.9

    Language:English  

    Venue:徳島  

    国際学会

  • Kazuhiro Shiozaki, Koichi Koseki, Kazunori Yamaguchi, Momo Shiozaki, Taeko Miyagi   Developmental change of Neu4 expression in mouse brain and its involvement in neuronal cell differentiation   International conference

    Sialoglycoscience 2006  Sialoglycoscience 2006

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    Event date: 2006.8

    Language:English  

    Venue:静岡  

    国際学会

  • 塩崎一弘、山並秀章、上村卓嗣、和田正、山口壹範、宮城妙子   新規シアリダーゼ(NEU4)による大腸がん細胞の接着・運動能の抑制とその機構  

    第26回日本糖質学会年会  第26回日本糖質学会年会

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    Event date: 2006.8

    Language:Japanese  

    Venue:宮城  

    国内学会

  • Kazuhiro Shiozaki, Koichi Koseki, Kazunori Yamaguchi, Momo Shiozaki, Taeko Miyagi   Expression of sialidase Neu4 in mouse brain development and its functional significance   International conference

    20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress  20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress

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    Event date: 2006.6

    Language:English  

    Venue:京都  

    国際学会

  • 塩崎一弘、山並秀章、和田正、山口壹範、宮城妙子   新規シアリダーゼ(NEU4)によるヒト大腸がん細胞の接着・運動能の制御  

    第71回日本生化学会東北支部会  第71回日本生化学会東北支部会

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    Event date: 2006.5

    Language:Japanese  

    Venue:青森  

    国内学会

  • 塩崎一弘、中野俊樹、山口敏康、佐藤 実   ニジマスに対するヒスタミンの影響とステビア成分の解毒機構  

    平成17年度日本水産学会東北支部会  平成17年度日本水産学会東北支部会

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    Event date: 2005.11

    Language:Japanese  

    Venue:宮城  

    国内学会

  • Kazuhiro Shiozaki, Hideaki Yamanami, Tadashi Wada, Kazunori Yamaguchi, Tsuneaki Fujiya, Taeko Miyagi   Down- regulation of sialidase NEU4 in human colon cancer and its pathological significance  

    第78回日本生化学会大会  第78回日本生化学会大会

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    Event date: 2005.10

    Language:Japanese  

    Venue:兵庫  

    国内学会

  • 塩崎一弘、中野俊樹、山口敏康、佐藤 実   キク科植物ステビア抽出物の養殖魚におけるヒスタミン解毒作用  

    財)漢方医薬研究振興財団 漢方医薬研究報告会  財)漢方医薬研究振興財団 漢方医薬研究報告会

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    Event date: 2004.10

    Language:Japanese  

    Venue:東京  

    研究会

  • Kazunori Yamaguchi, Tadashi Wada, Keiko Hata, Setsuko Moriya, Kazuhiro Shiozaki, Koichi Koseki, Taeko Miyagi   Characterization of the NEU4 sialidase  

    第77回日本生化学会大会  第77回日本生化学会大会

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    Event date: 2004.10

    Language:Japanese  

    Venue:神奈川  

    国内学会

  • 塩崎一弘、中野俊樹、山口敏康、佐藤実、佐藤直彦   ニジマスのヒスタミン代謝に及ぼすステビア抽出物の添加  

    平成16年度日本水産学会大会  平成16年度日本水産学会大会

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    Event date: 2004.4

    Language:Japanese  

    Venue:鹿児島  

    国内学会

  • 塩崎一弘、中野俊樹、山口敏康、佐藤実、佐藤直彦   ステビア抽出物のニジマスにおけるヒスタミン代謝酵素に与える影響について  

    平成15年度日本水産学会大会  平成15年度日本水産学会大会

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    Event date: 2003.4

    Language:Japanese  

    Venue:東京  

    国内学会

  • 塩崎一弘、藤井晃子、高橋計介、中野俊樹、山口敏康、佐藤実、竹内昌昭、渡辺和夫、佐藤直彦   ニジマスにおけるステビア抽出物のヒスタミン解毒有効画分とその作用  

    平成12年度日本水産学会大会  平成12年度日本水産学会大会

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    Event date: 2000.9

    Language:Japanese  

    Venue:福井  

    国内学会

  • 佐藤實、滝浪哲郎、塩崎一弘、山口敏康、熊谷勉、奚印慈、佐藤直彦   ステビア抽出物の抗酸化性分  

    第15回和漢医薬学会大会  第15回和漢医薬学会大会

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    Event date: 1998.8

    Language:Japanese  

    Venue:千葉  

    国内学会

  • 塩崎一弘、藤井晃子、佐藤実、高橋計介、渡辺和夫、佐藤直彦   ニジマスにおけるステビア抽出物のヒスタミン解毒有効画分とその作用  

    第15回和漢医薬学会大会  第15回和漢医薬学会大会

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    Event date: 1998.8

    Language:Japanese  

    Venue:千葉  

    国内学会

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Research Projects

  • E. tarda感染における魚類細胞の糖鎖リモデリングの意義解明

    2019.4 - 2022.3

    科学研究費補助金  基盤研究(C)

  • E. tarda感染における魚類細胞の糖鎖リモデリングの意義解明

    Grant number:19K06223  2019.4 - 2022.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    塩崎 一弘

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    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    本年度は、Edwardsiella tardaのNanAシアリダーゼとシアル酸代謝に注目して解析を行った。病原性の異なる菌株を用い、その感染能とNanAシアリダーゼ活性を比較したところ、病原性の高い株ほどNanA活性が高いことが明らかとなった。また、E. tardaゲノムをin silicoで解析したところ、2種のシアル酸代謝経路が予想された。ゲノム上では2つのN-neuraminate lyaseが存在しており、そのうち1つはNanA活性が低い株で高い発現が認められた。そこでシアル酸を培地に添加してE. tardaの増殖を検討したところ、グルコースの約半分の効率で栄養源として利用できることが分かり、Lyaseによるシアル酸分解が増殖に重要であることが明らかとなった。
    また、NanAにより遊離したシアル酸をCMPと結合させるCMP-NANA合成酵素の遺伝子発現を解析したところ、病原性の高い株で発現が亢進していた。そこで、E. tardaの菌体をリポ多糖(LPS)、糖タンパク質、糖脂質に分画し、そのシアル酸量を測定したところ、CMP-NANA合成酵素の発現パターンと同じく、高病原性株で高いシアル酸量を示した。興味深いことに、E. tardaのLPSにはかなりのシアル酸が含まれていることが判明し、他のバクテリアの報告と合わせて考えると、このシアル酸は宿主の免疫系細胞からの逃避に関与していることが推察された。本研究により、E. tardaのシアル酸を介した病原性発現のメカニズムが明らかになったことで、シアル酸代謝を標的としたE. tardaの感染抑制、治療薬の開発において有効な知見となると考えられる。

  • エドワジエラ症感染阻害物質を見つけだす迅速簡便な評価方法の開発

    Grant number:19H00295  2019

    日本学術振興会  科学研究費助成事業 奨励研究  奨励研究

    二神 裕子, 塩崎 一弘

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    Grant amount:\540000 ( Direct Cost: \540000 )

    E. tarda感染能評価のため1)GFP遺伝子導入E. tardaをGAKS細胞に感染2)E. tardaをGAKS細胞に感染後、DAPIでゲノムDNAを染色3)NanAシアリダーゼ活性染色の3つを比検討した。in vitroでは1)が感評価に適しており、in vivoではreal-time PCRでGFP遺伝子の検出を行う方法が適していた。またゼブラフィッシュ仔魚で同じ実験を行ったところ、体が透明なためGFP導入E. tardaの観察が容易で、迅速簡便な評価方法としてより適していた。

  • 糖鎖関連分子に注目した養殖魚エドワジエラ症の新規予防・治療薬開発に関する研究

    2018.4 - 2019.3

    サンケイ科学振興財団  研究助成 

    塩崎 一弘

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  • 魚類の摂餌行動に関与するpH感受性の分子細胞基盤の解明

    2017.4 - 2020.3

    文部科学省  科学研究費補助金:基盤(C) 

    池永隆徳

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  • 魚類の摂餌行動に関与するpH感受性の分子細胞基盤の解明

    Grant number:17K07943  2017.4 - 2020.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    池永 隆徳, 清原 貞夫, 塩崎 一弘

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    底棲性の海産真骨魚類であるゴンズイは海水の僅か0.1のpHの変化を感知し、これをゴカイなどの底質中の餌の探索に利用している。しかしながら、この鋭敏なpH感受性を担う受容体や細胞の種類は不明である。本研究では、この鋭敏なpH感受性を担う細胞や神経、および受容体を明らかにすることを目的としている。感覚刺激に対する細胞の応答は、生きた標本を用いた生理学的手法の他に、固定した標本において最初期遺伝子の一種であるc-fos遺伝子の発現を指標とする方法が利用されている。本年度は昨年度決定したゴンズイのc-fos遺伝子のcDNA配列をもとに作成したプローブを用いたin situ hybridization法を実施した。今回作成したプローブを用いたin situ hybridization法によって、神経細胞の活動依存的に発現するc-fos遺伝子の検出が可能であるかを検討するために、ペンチレンテトラゾール(PTZ) で処理したゴンズイ脳におけるc-fos遺伝子の発現をin situ hybridization法で検出することを試みた。また、未処理のゴンズイ脳に対しても同様にin situ hybridization法を実施し、これをPTZ処理したものと比較した。その結果、PTZで処理したゴンズイでは終脳や間脳を始め、脳全体でc-fos遺伝子を発現する細胞の数が増えていた。このことから、ゴンズイにおいてもc-fosの発現を指標に脳内の神経細胞の活動を検出することが可能であることが明らかとなった。また、触鬚より精製したmRNAを用いたトランスクリプトーム解析を開始した。

  • ゲノム編集技術を用いた魚類リソソームシアリダーゼの機能解析

    2016.4 - 2019.3

    科学研究費補助金  基盤研究(C)

  • ゲノム編集技術を用いた魚類リソソームシアリダーゼの機能解析

    2016.4 - 2019.3

    文部科学省  科学研究費補助金:基盤(C) 

    塩崎 一弘

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  • Establishment of Neu1 knockout zebrafish by genome-editing

    Grant number:16K07876  2016.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Shiozaki Kazuhiro

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    Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

    Mammalian sialidases have been studied so far. Among them, lysosomal sialidases are known to be involved in immunity, autophasy and sialidosis. On the other hand, the functions of fish lysosomal sialidase is not clear. Here, we tried to clarify the significance of fish lysosomal sialidase using gene-modified fish.
    First, Neu1 polypeptide knockdown using morpholino-oligo was conducted in medaka embryo. Knockdown embryo exhibited the accumulation of sialoglycoprotein and abnormal phenotypes such as higher heart rate. Enzymatic properties of fish Neu1 are found to be highly conserved by gene cloning and polypeptide analysis. Next, we tried to establish Neu1 knockout zebrafish by CRISPR/Cas9 method. Neu1 knockout zebrafish showed alteration of muscle- and bone-related gene expressions in embryo accompanied with the decrease of sialidase activity. Adult fish showed the abnormal bone shape. As a result, fish Neu1 would be crucial in bone formations.

  • 魚類における複合糖鎖および関連酵素の生理機能解析

    2012.4 - 2016.3

    科学研究費補助金  若手研究(B)

  • Expression of sialidase in cancer and its significance

    JST Basic Research Programs (Core Research for Evolutional Science and Technology :CREST) 

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  • シアリダーゼ制御による新規がん治療法・診断法の開発

    厚生労働省  厚生労働省科学研究費補助金 

    塩崎 一弘

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Teaching Experience

  • 高度分析・調査実習BCH

  • 食品科学基礎実験

  • 食品化学

  • 食品・資源利用学特別指導

  • 資源利用学演習

  • 生物化学実験

  • 水産食品科学

  • 水産学とキャリア

  • 情報活用

  • 応用生命科学特論

  • 大学と地域

  • 初年次セミナーI

  • 分子生物学

  • Latest Analytical and Experimental Method (Biochemistry)

    Institution:Faculty of Fisheries, Kagoshima University

  • Fisheries Products Utilization

    Institution:Faculty of Fisheries, Kagoshima University

  • Fisheries Production Science

    Institution:Faculty of Fisheries, Kagoshima University

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