Updated on 2023/10/20

写真a

 
Kaoru MITSUI
 
Organization
Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Neuro-musculoskeletal Disorder Associate Professor
Title
Associate Professor
Contact information
メールアドレス

Degree

  • 博士(医学) ( 2000.3   久留米大学 )

  • 修士(学術) ( 1996.3   筑波大学 )

Research Interests

  • Regenerative Medicine

  • Embryonic Stem Cells

  • induced Pluripotent Stem Cells

  • Viral Vector

Research Areas

  • Life Science / Tumor biology  / 再生医学

  • Life Science / Tumor biology  / 遺伝子治療・再生医学

  • Life Science / Cell biology  / 幹細胞生物学

  • Life Science / Anatomy  / 組織学

  • Life Science / Developmental biology

  • Life Science / Molecular biology

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Education

  • Kurume University   Graduate School of Medicine

    1996.4 - 2000.3

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    Country: Japan

  • University of Tsukuba   Graduate School of Biosystem

    1994.4 - 1996.3

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    Country: Japan

  • University of Yamanashi   Faculty of Education

    1990.4 - 1994.3

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    Country: Japan

Research History

  • Kagoshima University   Graduate School of Medical and Dental Sciences   Associate Professor

    2019.1

  • Kagoshima University   Graduate School of Medical and Dental Sciences   Lecturer

    2009.7 - 2018.12

  • Saitama Medical University   The Research Center for Genomic Medicine   Assistant Professor

    2006.4 - 2009.6

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    Country:Japan

  • University of Yamanashi

    2005

  • National Institute of Genetics   Assistant Professor

    2003.5 - 2006.3

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    Country:Japan

  • Nara Institute of Science and Technology   Assistant Professor

    2000.4 - 2003.4

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    Country:Japan

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Professional Memberships

  • 日本解剖学会

    2013.6

  • 再生医療学会

    2000.3

  • 分子生物学会

    1996.3

 

Papers

  • Mitsui K, Takahashi T, Ide K, Matsuda E, Kosai KI .  Optimization of adenoviral gene transfer in human pluripotent stem cells. .    541   78 - 83   2021.2Reviewed International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2021.01.009

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    PubMed

  • Ide K, Mitsui K, Irie R, Matsushita Y, Ijichi N, Toyodome S, Kosai KI .  A Novel Construction of Lentiviral Vectors for Eliminating Tumorigenic Cells from Pluripotent Stem Cells. .  Stem cells (Dayton, Ohio)36 ( 2 ) 230 - 239   2018.2Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/stem.2725

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    PubMed

  • Kaoru Mitsui, Kanako Ide, Tomoyuki Takahashi, Ken-ichiro Kosai .  Viral Vector-Based Innovative Approaches to Directly Abolishing Tumorigenic Pluripotent Stem Cells for Safer Regenerative Medicine .  MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT5   51 - 58   2017.6Invited Reviewed International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:CELL PRESS  

    Human pluripotent stem cells (hPSCs) are a promising source of regenerative material for clinical applications. However, hPSC transplant therapies pose the risk of teratoma formation and malignant transformation of undifferentiated remnants. These problems underscore the importance of developing technologies that completely prevent tumorigenesis to ensure safe clinical application. Research to date has contributed to establishing safe hPSC lines, improving the efficiency of differentiation induction, and indirectly ensuring the safety of products. Despite such efforts, guaranteeing the clinical safety of regenerative medicine products remains a key challenge. Given the intrinsic genome instability of hPSCs, selective growth advantage of cancer cells, and lessons learned through failures in previous attempts at hematopoietic stem cell gene therapy, conventional strategies are unlikely to completely overcome issues related to hPSC tumorigenesis. Researchers have recently embarked on studies aimed at locating and directly treating hPSC-derived tumorigenic cells. In particular, novel approaches to directly killing tumorigenic cells by transduction of suicide genes and oncolytic viruses are expected to improve the safety of hPSC-based therapy. This article discusses the current status and future perspectives of methods aimed at directly eradicating undifferentiated tumorigenic hPSCs, with a focus on viral vector transduction.

    DOI: 10.1016/j.omtm.2017.03.002

    Web of Science

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    PubMed

  • Kaoru Mitsui, Kanako Ide, Akiko Takayama, Tadahisa Wada, Rie Irie, Ken-ichiro Kosai .  Conditionally replicating adenovirus prevents pluripotent stem cell–derived teratoma by specifically eliminating undifferentiated cells .  Molecular Therapy — Methods & Clinical Development 2   2015.8Reviewed International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/mtm.2015.26

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  • Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S. .  The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. .  Cell.113 ( 5 ) 631 - 642   2003.5Reviewed International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0092-8674(03)00393-3

  • Kiyonori Tanoue, Yuqing Wang, Minako Ikeda, Kaoru Mitsui, Rie Irie, Takao Setoguchi, Setsuro Komiya, Shoji Natsugoe, Ken-ichiro Kosai .  Survivin-responsive conditionally replicating adenovirus kills rhabdomyosarcoma stem cells more efficiently than their progeny. .  Journal of Translational Medicine12 ( 1 ) 1479 - 5876   2014.1Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/1479-5876-12-27

  • Mitsui K, Suzuki K, Aizawa E, Kawase E, Suemori H, Nakatsuji N, Mitani K .  Gene targeting in human pluripotent stem cells with adeno-associated virus vectors. .  Biochemical and Biophysical Research Communications388 ( 4 ) 711 - 717   2009.10Reviewed International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2009.08.075

  • Kawasaki T, Saito K, Mitsui K, Ikawa M, Yamashita M, Taniguchi Y, Takeda S, Mitani K, Sakai N. .  Introduction of a foreign gene into zebrafish and medaka cells using adenoviral vectors. .  Zebrafish6 ( 3 ) 253 - 258   2009.9Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/zeb.2009.0596

  • Suzuki K, Mitsui K, Aizawa E, Hasegawa K, Kawase E, Yamagishi T, Shimizu Y, Suemori H, Nakatsuji N, Mitani K. .  Highly efficient transient gene expression and gene targeting in primate embryonic stem cells with helper-dependent adenoviral vectors. .  Proceedings of the National Academy of Sciences of the United States of America105 ( 37 ) 13781 - 13786   2008.9Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.0806976105

  • Watanabe S, Mitsui K, Nakayama T. Inouye I .  Phylogenetic relationships and taxonomy of sarcinoid green algae: Chlorosarcinopsis, Desmotetra, Sarcinochlamys gen. nov., Neochlorosarcina, and Chlorosphaeropsis (Chlorophyceae, Chlorophyta) .  Journal of Phycology42 ( 3 ) 679 - 695   2006.6Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Phycology  

    We investigated the phylogenetic relationships and the taxonomy of 20 sarcinoid strains by their morphological features and using 18S rDNA sequence data. Nineteen strains of Chlorosarcinopsis (Gerneck) Herndon and allied genera were divided into four groups (A-D). Group A, comprising eight species, was concordant with the description of Chlorosarcinopsis and divided into four polyphyletic subgroups. Group B included two species of Chlorosarcinopsis and two strains of Desmotetra stigmatica (Deason) Deason et Floyd, all of which had parallel thylakoid membranes in pyrenoid matrices, and zoospores bearing apical stigma and subapical flagellar apparatus. The definition of Desmotetra Deason et Floyd was emended to include these features, and Chlorosarcinopsis delicata S. Watanabe was transferred to this genus. Group C comprised Chlorosarcina stigmatica Deason (ASIB.T105), for which we proposed Sarcinochlamys gen. nov., a genus distinct from Chlorosarcina Gerneck in having walled zoospores. Group D, comprising six species, corresponded to Neochlorosarcina and was divided into two subgroups. The presence of thin cell walls in Neochlorosarcina S. Watanabe was ascertained as a valid feature for circumscribing the sarcinoid genera. The physiological experiments on the species of Chlorosarcinopsis and Chlorosarcina by Groover and Bold (1968) were assessed based on the phylogenetic results. Groups (A, B, D) were roughly characterized by these features: algal mass color, utilization of selected nitrogen compounds and carbon sources under light/dark and aerobic/anaerobic conditions, and requirement for vitamin B12. Molecular analysis revealed that Chlorosphaeropsis alveolata Herndon had closer affinity with solitary Protosiphon Klebs and Spongiochloris Starr than with sarcinoid members. © 2005 Phycological Society of America.

    DOI: 10.1111/j.1529-8817.2006.00196.x

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  • Takahashi K, Mitsui K, Yamanaka S. .  Role of ERas in promoting tumour-like properties in mouse embryonic stem cells. .  Nature.423 ( 6939 ) 541 - 545   2003.5Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nature01646

  • Tokuzawa Y, Kaiho E, Maruyama M, Takahashi K, Mitsui K, Maeda M, Niwa H, Yamanaka S. .  Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. .  Molecular and Cellular Biology23 ( 8 ) 2699 - 2708   2003.4Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/MCB.23.8.2699-2708.2003

  • Mitsui K, Nakanishi M, Ohtsuka S, Norwood TH, Okabayashi K, Miyamoto C, Tanaka K, Yoshimura A, and Ohtsubo M. .  A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins .  Biochemical and Biophysical Research Communications266 ( 1 ) 115 - 122   1999.12Reviewed International coauthorship International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Biochemical and Biophysical Research Communications  

    Cyclin E-Cdk2 is an evolutionary conserved cyclin-dependent kinase (CDK) complex that drives the G1 to S phase transition of the cell cycle. A novel cDNA encoding a HECT family protein also containing RCC1-like repeats was isolated by a yeast two-hybrid screening using both cyclin E and its inhibitor p21. The protein product of this cDNA, Ceb1, interacts with various cyclin subunits of CDKs in mammalian cells. Expression of Ceb1 is specifically detected in testis and ovary and is highly elevated when the functions of the tumor suppressor proteins, p53 and RB, are compromised by mutations or viral oncoproteins. The present results suggest that Ceb1 may play a critical role when its expression and the CDK activity are upregulated by inactivation of p53 and RB.

    DOI: 10.1006/bbrc.1999.1777

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  • Mitsui K, Matsumoto A, Ohtsuka S, Ohtsubo M, and Yoshimura A .  Cloning and characterization of a novel p21(Cip1/Waf1)-interacting zinc finger protein, Ciz1 .    264 ( 2 ) 457 - 464   1999.10Reviewed International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/bbrc.1999.1516

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  • Tanimura S, Ohtsuka S, Mitsui K, Shirouzu K, Yoshimura A, and Ohtsubo M. .  MDM2 interacts with MDMX through their RING finger domains .  FEBS Letters447 ( 1 ) 5 - 9   1999.3Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FEBS Letters  

    The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized with MDMX through their C-terminal RING finger domains. Yeast two-hybrid analysis revealed that the hetero-oligomerization between MDMX and MDM2 was more stable than the homo-oligomerization of each protein. MDM2 has been shown to be degraded by the ubiquitin-proteasome pathway, while MDMX was a stable protein. Interaction of MDMX with MDM2 through the C-terminal RING finger domains resulted in inhibiting degradation of MDM2. These data indicate that MDMX functions as a regulator of MDM2. Copyright (C) 1999 Federation of European Biochemical Societies.

    DOI: 10.1016/S0014-5793(99)00254-9

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  • Wakioka T, Sasaki A, Mitsui K, Yokouchi M, Inoue A, Komiya S, and Yoshimura A. .  APS, an adaptor protein containing Pleckstrin homology (PH) and Src homology-2 (SH2) domains inhibits the JAK-STAT pathway in collaboration with c-Cbl .  Leukemia13 ( 5 ) 760 - 767   1999Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Leukemia  

    We cloned a novel adaptor protein, APS (adaptor molecule containing Pleckstrin homology (PH) and Src Homology-2 (SH2) domains), which was tyrosine phosphorylated in response to c-kit or B cell receptor stimulation. Here, we report that APS was tyrosine phosphorylated by Janus kinase-2 (JAK2) at its C-terminal tyrosine residue and interacted with c-Cbl. Forced expression of APS in an erythropoietin (EPO)-dependent hematopoietic cell line resulted in reduced activation of STAT5 but not cell proliferation in response to EPO. APS bound to the phosphorylated tyrosine residue, Y343 of the erythropoietin receptor cytoplasmic domain. Co-expression of APS and c-Cbl, but not expression of either alone inhibited EPO-dependent STAT5 activation in 293 cells. This required the C-terminal phosphorylation site, as well as PH and SH2 domains of APS. Therefore, one of the major functions of APS is in recruitment of c-Cbl into the receptor/JAK complex, thereby inhibiting JAK signaling activity.

    DOI: 10.1038/sj.leu.2401397

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  • Masuhara M, Sakamoto H, Matsumoto A, Suzuki R, Yasukawa H, Mitsui K, Wakioka T, Tanimura S, Sasaki A, Misawa H, Yokouchi M, Ohtsubo M, and Yoshimura A. .  Cloning and characterization of novel CIS family genes .    239 ( 2 ) 439 - 446   1997.10Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/bbrc.1997.7484

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  • Endo TA, Masuhara M, Yokouchi M, Suzuki R, Sakamoto H, Mitsui K, Matsumoto A, Tanimura S, Ohtsubo M, Misawa H, Miyazaki T, Leonor N, Taniguchi T, Fujita T, Kanakura Y, Komiya S, and Yoshimura A. .  A new protein containing an SH2 domain that inhibits JAK kinases .  Nature387 ( 6636 ) 921 - 924   1997.6Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Nature  

    The proliferation and differentiation of cells of many lineages are regulated by secreted proteins known as cytokines. Cytokines exert their biological effect through binding to cell-surface receptors that are associated with one or more members of the JAK family of cytoplasmic tyrosine kinases. Cytokine-induced receptor dimerization leads to the activation of JAKs, rapid tyrosine-phosphorylation of the cytoplasmic domains, and subsequent recruitment of various signaling proteins, including members of the STAT family of transcription factors, to the receptor complex. Using the yeast two-hybrid system, we have now isolated a new SH2-domain-containing protein, JAB, which is a JAK-binding protein that interacts with the Jak2 tyrosine-kinase JH1 domain. JAB is structurally related to CIS, a cytokine- inducible SH2 protein. Interaction of JAB with Jak1, Jak2 or Jak3 markedly reduces their tyrosine-kinase activity and suppresses the tyrosine- phosphorylation and activation of STATs. JAB and CIS appear to function as negative regulators in the JAK signalling pathway.

    DOI: 10.1038/43213

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  • Matsumoto A, Masuhara M, Mitsui K, Yokouchi M, Ohtsubo M, Misawa H, Miyajima A, and Yoshimura A. .  CIS, a cytokine inducible SH2 protein, is a target of the JAK-STAT5 pathway and modulates STAT5 activation .  Blood89 ( 9 ) 3148 - 3154   1997.5Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Blood  

    We searched for immediate early cytokine responsive genes and isolated a novel gene, CIS (Cytokine Inducible SH2 containing protein) that is induced in hematopoietic cells by a subset of cytokines including interleukin-2 (IL- 2), IL-3, and erythropoietin (EPO). The mutant IL-2 receptor that fails to activate STAT5 could not induce CIS, suggesting that STATS is involved in the cytokine-inducible expression of CIS. We cloned the 5'-flanking region of the CIS gene and found that about 200 bases upstream of the transcription- initiation site contain four potential STATS binding sites (MGF boxes). Luciferase reporter assays showed that these MGF boxes were essential for EPO-dependent promoter activity. Expression of STAT5 and the EPO receptor in HEK293 cells conferred EPO-dependent activation of the CIS promoter. These data indicate that CIS is a target of the JAK-STAT5 pathway of cytokine receptors. CIS contains an SH2 domain and binds to tyrosine-phosphorylated EPO and IL-3 receptors. In HEK293 cells expressing STAT5 and the EPO receptor, EPO-dependent tyrosine phosphorylation of STAT5, as well as EPO- dependent CIS-promoter activation, was suppressed when CIS was coexpressed. Moreover, the induction of oncostatin M, another STAT5 target, as well as the tyrosine-phosphorylation of STAT5, were partially suppressed by CIS expression in Ba/F3 cells. Thus, CIS is a feedback modulator of STAT5; its expression is induced by STAT5 and it negatively modulates STAT5 activation.

    DOI: 10.1182/blood.v89.9.3148

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  • Nakayama T, Watanabe S, Mitsui K, Uchida H, and Inouye I. .  The phylogenetic relationship between the Chlamydomonadales and Chlorococcales inferred from 18SrDNA sequence data. .  Phycological Research44 ( 1 ) 47 - 55   1996Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:John Wiley & Sons  

    DOI: 10.1111/j.1440-1835.1996.tb00037.x

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Books

  • iPS細胞の安全・高品質な作製技術

    三井 薫、小戝健一郎、他( Role: Joint author ,  第1章 iPS細胞作製・培養時における細胞のがん化メカニズムと検出・評価 【第5節 ウイルスを用いたがん化細胞(腫瘍化原因細胞)の除去技術の開発】)

    技術情報協会  2016.10 

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    Total pages:494   Responsible for pages:38-45   Language:Japanese Book type:Scholarly book

MISC

  • iPS細胞培養からがん化の恐れのある細胞を死滅させる方法

    三井 薫、小戝 健一郎

    PHARMSTAGE   15 ( 12 )   10 - 15   2016.3

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:技術情報協会  

  • A novel method using conditionally replicating adenovirus for specifically abolishing tumorigenic cells in pluripotent stem cells.

    Kaoru Mitsui, Ken-ichiro Kosai

    BIOINDUSTRY   33 ( 2 )   11 - 18   2016.2

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:CMC Publishing CO., LTD  

Presentations

  • 西川路侑耶、川上広高、小浜裕行、松田恵理子、三井薫、渡邉真季、小戝 健一郎   至適プロモーター制御下でサイトカイン遺伝子を発見する安全で効果的な腫瘍溶解性ウイルスの開発.  

    日本解剖学会第78回九州支部学術集会  2022.10  日本解剖学会第78回九州支部学術集会

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    Event date: 2022.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡  

  • 三井薫、小戝健一郎   腫瘍溶解性アデノウイルスにおける多能性幹細胞・再生医療の腫瘍化阻止技術.  

    日本解剖学会第78回九州支部学術集会  2022.10  日本解剖学会第78回九州支部学術集会

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    Event date: 2022.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡  

  • Kaoru Mitsui, Eriko Matsuda, Ken-ichiro Kosai.   Development of a method using oncolytic adenovirus to specifically eliminate tumorigenic cells in pluripotent stem cell-based regenerative medicine.   International conference

    14th International Oncolytic Virotherapy Conference(IOVC2022)  2022.10  14th International Oncolytic Virotherapy Conference(IOVC2022)

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    Event date: 2022.10

    Language:English   Presentation type:Oral presentation (general)  

  • 三井薫、伊地知暢広、井手佳菜子、松田恵理子、小戝健一郎   鹿児島大学でのCOVID-19下でのオンライン組織学実習への取り組み.   Invited

    第127回日本解剖学会総会・全国学術集会  2022.3  第127回日本解剖学会総会・全国学術集会

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    Event date: 2022.3

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:東京・オンライン  

  • 三井薫、高橋知之、井手佳菜子、松田恵理子、小戝健一郎   アデノウイルスベクターを用いたヒト多能性幹細胞への高効率遺伝子導入法.  

    日本解剖学会第77回九州支部学術集会  2021.10  日本解剖学会第77回九州支部学術集会

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大分・オンライン  

  • Optimization of Highly Efficient Gene Transfer with Adenoviral Vectors in Human Pluripotent Stem Cells.  

    2021.9 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 西川路侑耶、伊地知暢広、三井薫、小戝健一郎   次世代の腫瘍溶解性ウイルスの開発と癌と再生医学への治療応用.  

    日本解剖学会第76回九州支部学術集会  2020.10  日本解剖学会第76回九州支部学術集会

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    Event date: 2020.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島・オンライン  

  • 三井薫、伊地知暢広、井手佳菜子、小戝健一郎   COVID-19下でのオンライン組織学実習への取り組み.   Invited

    日本解剖学会第76回九州支部学術集会  2020.10  日本解剖学会第76回九州支部学術集会

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    Event date: 2020.10

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:鹿児島・オンライン  

  • 三井薫、小戝健一郎   多能性幹細胞の腫瘍化原因細胞除去技術へのゲノム編集の応用.(  

    第4回日本遺伝子細胞治療学会若手研究会セミナー  2019.11  第4回日本遺伝子細胞治療学会若手研究会セミナー

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京  

  • 井手佳菜子、三井薫、松田恵理子、小戝健一郎   独自開発のレンチウイルスベクター(TC-LV)による多能生幹細胞の腫瘍化原因細胞の同定・除去技術.  

    第75回日本解剖学会九州支部学術集会  2019.11  第75回日本解剖学会九州支部学術集会

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡  

  • 井手佳菜子、豊留宗一郎、三井薫、松田恵理子、小戝健一郎   多能生幹細胞の腫瘍化完全克服を目指した「ゲノム編集での自殺遺伝子挿入技術」の開発.(  

    第18回日本再生医療学会  2019.3  第18回日本再生医療学会

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:兵庫  

  • 井手佳菜子、三井薫、松田恵理子、小戝健一郎   多能性幹細胞での再生医療に必須となる腫瘍化克服の独自技術の開発.  

    第3回日本遺伝子細胞治療学会若手研究会セミナー   2018.12  第3回日本遺伝子細胞治療学会若手研究会セミナー

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    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京  

  • Ide K, Mitsui K, Kosai K   A novel construction of lentiviral vectors that identify and eliminate tumorigenic cells from pluripotent stem cells.   International conference

    Internatinal Society for Stem Cell Research (ISSCR) 2018 Annual Meeting  Internatinal Society for Stem Cell Research (ISSCR)

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    Event date: 2018.6

    Language:English   Presentation type:Poster presentation  

    Venue:Melbourne, Australia  

  • 三井薫、井手佳菜子、豊留宗一郎、小戝健一郎   多能性幹細胞由来腫瘍を直接殺傷除去する革新的なウイルスベクター技術の開発  

    第35回日本ヒト細胞学会学術集会  日本ヒト細胞学会

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    Event date: 2017.10

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:種子島、鹿児島県  

  • Mitsui Kaoru, Ide Kanako, Kosai Ken-ichiro   Viral Vector-based Innovative Approaches to Directly Abolishing Tumorigenic Cells: Novel Technologies for Safer Clinical Application of Pluripotent Stem Cell?based Regenerative Medicine  

    第23回日本遺伝子細胞治療学会学術集会  日本遺伝子細胞治療学会

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    Event date: 2017.7

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:岡山  

  • 三井 薫、井手 佳菜子、小戝 健一郎   独自開発の増殖制御型アデノウイルスベクターによる新たな多能性幹細胞の腫瘍化阻止技術の開発  

    第122回日本解剖学会総会全国学術集会  日本解剖学会

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎県長崎市  

  • 井手 佳菜子、三井 薫、小戝 健一郎   多能性幹細胞の腫瘍化阻止のための新規レンチウイルスベクター技術の開発  

    第122回日本解剖学会総会全国学術集会  日本解剖学会

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎県長崎市  

  • 小戝 健一郎、三井 薫、井手 佳菜子、伊地知 暢広、入江 理恵   遺伝子治療、再生医療の独自開発技術の臨床応用と発生学への展望  

    第122回日本解剖学会総会全国学術集会  日本解剖学会

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎県長崎市  

  • 井手 佳菜子、三井 薫、小戝 健一郎   多能性幹細胞の腫瘍化細胞を同定・殺傷する新規レンチウイルスベクターの効率的作製法の開発  

    第16回日本再生医療学会総会  日本再生医療学会

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮城県仙台市  

  • 三井 薫、井手 佳菜子、小戝 健一郎   独自開発の多因子制御増殖型アデノウイルスによる多能性幹細胞の腫瘍化阻止の新技術?心筋分化系での検討  

    第16回日本再生医療学会総会  日本再生医療学会

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮城県仙台市  

  • 三井 薫、井手 佳菜子、小戝 健一郎   多能性幹細胞による再生医療の腫瘍化を根絶する新戦略:腫瘍化原因細胞を直接標的治療する独自ウイルスベクター技術の開発  

    第1回 日本遺伝子治療学会若手研究会セミナー  日本遺伝子治療学会若手研究会

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    Event date: 2016.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都  

  • 井手 佳菜子、三井 薫、小戝 健一郎   多能性幹細胞の腫瘍化細胞を同定・標的殺傷する新規レンチウイルスヘ゛クターの開発   International conference

    第39回 日本分子生物学会年会  日本分子生物学会

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    Event date: 2016.11 - 2016.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神奈川県横浜市  

  • 小戝 健一郎、三井 薫、井手 佳菜子   革新的か゛ん治療と幹細胞再生医療の腫瘍化克服を実現する独自開発の遺伝子治療・ウイルスヘ゛クター技術と応用   International conference

    第39回 日本分子生物学会年会  日本分子生物学会

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    Event date: 2016.11 - 2016.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神奈川県横浜市  

  • 三井 薫、井手 佳菜子、小戝 健一郎   独自開発の増殖制御型アテ゛ノウイルスヘ゛クターによる新たな多能性幹細胞の腫瘍化阻止技術の開発   International conference

    第39回 日本分子生物学会年会  日本分子生物学会

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    Event date: 2016.11 - 2016.12

    Language:English   Presentation type:Poster presentation  

    Venue:神奈川県横浜市  

  • Shiori Waki, Hisashi Sahara, Kaoru Mitsui, Mitsuhiro Sekijima, Takahiro Murokawa, Takehiro Iwanaga, Yurika Ichinari, Kenichiro Kosai, Kazuhiko Yamada   Efficacious combination of substrate and medium for feeder-free culture of naive porcine iPS   International conference

    The 26th International Congress of The Transplantation Society  The Transplantation Society

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    Event date: 2016.8

    Language:English   Presentation type:Poster presentation  

    Venue:Hong Kong  

  • Kaoru Mitsui, Kanako Ide, Ken-ichiro Kosai   Conditionally replicating adenovirus specifically eliminates pluripotent stem cell-derived tumorigenic cells without adverse effects to differentiated cardiomyocytes  

    第22回日本遺伝子細胞治療学会  日本遺伝子細胞治療学会

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    Event date: 2016.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京  

  • Kanako Ide, Kaoru Mitsui, Ken-ichiro Kosai   A Novel System to Efficiently Construct Lentiviral Vectors that Identify and Eliminate Tumorigenic Cells in Pluripotent Stem Cells  

    第22回日本遺伝子細胞治療学会  日本遺伝子細胞治療学会

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    Event date: 2016.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京  

  • Ken-ichiro Kosai, Kanako Ide, Kaoru Mitsui   AN EFFICIENT CONSTRUCTION OF LENTIVIRAL VECTORS THAT IDENTIFY AND ELIMINATE TUMORIGENIC CELLS DERIVED FROM PLURIPOTENT STEM CELLS   International conference

    ISSCR 2016 ANNUAL MEETING  International Society for Stem Cell Research

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    Event date: 2016.6

    Language:English   Presentation type:Poster presentation  

    Venue:San Francisco  

  • Kaoru Mitsui, Kanako Ide, Ken-ichiro Kosai   A NOVEL METHOD USING CONDITIONALLY REPLICATING ADENOVIRUS FOR SPECIFICALLY KILLING TUMORIGENIC CELLS DERIVED FROM PLURIPOTENT STEM CELLS   International conference

    ISSCR 2016 ANNUAL MEETING  International Society for Stem Cell Research

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    Event date: 2016.6

    Language:English   Presentation type:Poster presentation  

    Venue:San Francisco  

  • Kanako Ide, Kaoru Mitsui, Ken-ichiro Kosai   An Efficient Construction of Lentiviral Vectors That Identify and Eliminate Tumorigenic Cells in Pluripotent Stem Cells   International conference

    ASGCT 19th Annual Meeting  The American Society of Gene & Cell Therapy

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    Event date: 2016.5

    Language:English   Presentation type:Poster presentation  

    Venue:Washington, DC  

  • 三井 薫、井手 佳菜子、小戝 健一郎   ヒトES/iPS細胞における再生医療の課題を克服する独自ウイルスベクターの開発  

    第121回日本解剖学会総会全国学術集会  日本解剖学会

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    Event date: 2016.3

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福島県郡山市  

  • 井手 佳菜子,三井 薫,小戝 健一郎   ES/iPS細胞の腫瘍化細胞を可視化・標的殺傷するレンチウイルスベクターの開発と治療効果  

    第15回日本再生医療学会総会  日本再生医療学会

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪  

  • 三井 薫、井手 佳菜子、小戝 健一郎   独自開発の増殖制御型アデノウイルスベクターによるES/iPS細胞の腫瘍化細胞治療技術の発展  

    第15回日本再生医療学会総会  日本再生医療学会

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪  

  • Anefficientconstructionoflentiviralvectorsthatidentifyand eliminate tumorigenic cells in pluripotent stem cells  

    The 74th Annual Meeting of the Japanese Cancer Association  JCA

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    Event date: 2015.10

    Language:English   Presentation type:Poster presentation  

  • Oncolytic adenovirus vector, m-CRA, eliminates tumorigenic pluripotent stem cells  

    The 74th Annual Meeting of the Japanese Cancer Association  JCA

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    Event date: 2015.10

    Language:English   Presentation type:Poster presentation  

  • Ide K., Mitsui K., Matsushita Y., Kosai K.   An efficient construction of lentiviral vectors that identify and eliminate tumorigenic cells in pluripotent stem cells.  

    第21回日本遺伝子治療学会総会  日本遺伝子治療学会

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    Event date: 2015.7

    Language:English   Presentation type:Poster presentation  

    Venue:大阪  

  • Mitsui K., Ide K., Takayama A., Wada T., Irie R., Wang Y., Kosai K.   Conditionally replicating adenovirus kills tumorigenic pluripotent stem cells.  

    第21回日本遺伝子治療学会総会  日本遺伝子治療学会

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    Event date: 2015.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪  

    Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are promising sources of material for use in cell transplantation therapy. However, the risk of formation of tumors, including teratomas and cancers originating from contaminating undifferentiated and transformed cells, represents the most critical obstacle to the safe clinical application of hPSC-based regenerative medicine. Multiple approaches have been taken to improve safety by reducing the risk of carcinogenesis. However, most previous studies focused on improving generation of hiPSCs by eliminating potential oncogenic factors, such as the oncogene c-myc, or by integrating the reprogramming transgenes into chromosomes. Although these sorts of strategies, classified here as the first safety approach, reduce the reprogramming-associated oncogenic potential of hiPSCs, they cannot completely eliminate tumorigenic potentials due to the intrinsic characteristics of hPSCs. We previously developed a novel method (adenoviral conditional targeting) that securely isolated target cells from other cell types and undifferentiated hPSCs (Mol Ther. 14(5): 673-683. 2006 ; Cover & News in the issue of the journal). This method, which can increase the efficacy and safety of hPSC-based regenerative medicine by decreasing tumorigenicity, is classified here as the second safety approach. Strategies that can directly target and kill, rather than merely inhibit, tumorigenic cells are classified here as the third safety approach (See Ide’s presentation). Conditionally replicating adenoviruses (CRAs), also called oncolytic adenoviruses, can selectively replicate in and kill cancer cells; consequently, CRAs represent attractive anticancer drugs. Previously, we developed a method for generating CRAs that can target cancers with multiple cancer-specific factors (m-CRAs); this approach further increased cancer specificity without reducing the anticancer effects. We also demonstrated that among candidate m-CRAs, survivin-responsive m-CRA (Surv.m-CRA) is one of the most promising anticancer agents, in two respects: superior cancer specificity (i.e., safety) and therapeutic efficacy relative to clinically tested telomerase reverse transcriptase (TERT)-responsive m-CRAs (Tert.m-CRAs), and strong anticancer effects against currently incurable cancer stem cells. Based on our careful and extensive preclinical studies, we have a plan to start the investigator initiated first-in-human clinical trial of Surv.m-CRA for malignant orthopedic tumors after J-IND approval, hopefully, this year (as presented in a preclinical symposium session). Here, we demonstrate that m-CRAs may also be useful as novel antitumorigenic agents in hPSC-based therapy. We show that the survivin promoter was more active in undifferentiated hPSCs than the TERT promoter, whereas both promoters were minimally active in differentiated normal cells. Accordingly, Surv.m-CRA killed undifferentiated hPSCs more efficiently than Tert.m-CRA; both m-CRAs exhibited efficient viral replication and cytotoxicity in undifferentiated hPSCs, but not in co-cultured differentiated normal cells. Pre-infection of hPSCs with Surv.m-CRA or Tert.m-CRA abolished in vivo teratoma formation in a dose- dependent manner following hPSC implantation into mice. Thus, we demonstrate that m-CRAs, in particular Surv.m-CRAs, are potentially useful as both potent anticancer drugs and as novel antitumorigenic agents in hPSC-based regenerative medicine; in the latter context, the viruses act by specifically killing contaminating undifferentiated hPSCs.

  • 井手佳菜子、三井薫、松下洋平、小戝健一郎   ES/iPS細胞の腫瘍化細胞を可視化・標的殺傷するレンチウイルスベクターの効率的作製法の開発  

    第14回日本再生医療学会総会  第14回日本再生医療学会総会

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    Event date: 2015.3

    Language:Japanese  

    Venue:神奈川  

    国内学会

  • 三井薫、井手佳菜子、高山明子、和田忠久、小戝健一郎   独自開発の増殖制御型アデノウイルスベクターによる新たなES/iPS細胞の腫瘍化細胞治療技術の開発  

    第14回日本再生医療学会総会  第14回日本再生医療学会総会

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    Event date: 2015.3

    Language:Japanese  

    Venue:神奈川  

    国内学会

  • 三井薫、井手佳菜子、王宇清、入江理恵、小戝健一郎   独自開発の増殖制御型アデノウイルスベクターを用いた新たなES/iPS細胞の腫瘍化細胞除去方法の開発  

    第8回桜ヶ丘地区(基礎系)研究発表会  第8回桜ヶ丘地区(基礎系)研究発表会

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    Event date: 2015.1

    Language:Japanese  

    Venue:鹿児島  

    研究会

  • 三井薫、高橋知之、井手佳菜子、小戝健一郎   アデノウイルスベクターでのヒト多能性幹細胞への高効率遺伝子導入技術の開発  

    第13回日本再生医療学会総会  第13回日本再生医療学会総会

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    Event date: 2014.3

    Language:Japanese  

    Venue:京都  

    国内学会

  • 松下洋平、井手佳菜子、三井薫、小戝健一郎   多能性幹細胞の再生医療応用における新規腫瘍化阻止技術の開発(2)マウス ES 細胞 での検証  

    第7回 桜ヶ丘地区基礎系研究発表会  第7回 桜ヶ丘地区基礎系研究発表会

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    Event date: 2014.1

    Language:Japanese  

    Venue:鹿児島  

    研究会

  • 井手佳菜子、三井薫、小戝健一郎   多能性幹細胞の再生医療応用における新規腫瘍化阻止技術の開発(1)ヒト ES 細胞での検証  

    第7回 桜ヶ丘地区基礎系研究発表会  第7回 桜ヶ丘地区基礎系研究発表会

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    Event date: 2014.1

    Language:Japanese  

    Venue:鹿児島  

    研究会

  • 井手佳菜子、三井薫、小戝健一郎   増殖型アデノウイルスベクターを用いた安全なヒトES/iPS細胞治療の開発  

    第6回 桜ヶ丘地区基礎系研究発表会  第6回 桜ヶ丘地区基礎系研究発表会

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    Event date: 2013.1

    Language:Japanese  

    Venue:鹿児島  

    研究会

  • Kiyonori Tanoue, Yuqing Wang, Minako Ikeda, Kaoru Mitsui, Takao Setoguchi, Setsuro Komiya, Shoji Natsugoe and Ken-ichiro Kosai.   SURVIVIN-RESPONSIVE CONDITIONALLY REPLICATING ADENOVIRUS EFFICIENTLY KILLS RHABDOMYOSARCOMA-INITIATING CELLS: APROMISING m-CRA STRATEGY FOR TREATING CANCER STEM CELLS  

    第18回日本遺伝子治療学会学術集会  第18回日本遺伝子治療学会学術集会

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    Event date: 2012.6

    Language:Japanese  

    Venue:熊本  

    国内学会

  • 田上聖徳、王宇清、池田美奈子、三井薫、瀬戸口啓夫、小宮節郎、夏越祥次、小戝健一郎   独自開発の増殖型アデノウイルスベクターで癌幹細胞は効果的に治療できる  

    第11回日本再生医療学会総会  第11回日本再生医療学会総会

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    Event date: 2012.6

    Language:Japanese  

    Venue:神奈川  

    国内学会

  • 小戝健一郎、三井薫、王宇清、高橋知之   ヒトES/iPS細胞での再生医療の課題を克服する独自のアデノウイルスベクターの発現技術の開発  

    第11回日本再生医療学会総会  第11回日本再生医療学会総会

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    Event date: 2012.6

    Language:Japanese  

    Venue:神奈川  

    国内学会

  • 三井薫、井手佳菜子、高山明子、小戝健一郎   増殖型アデノウイルスベクターを用いた安全なヒトES/iPS細胞治療の開発  

    第11回日本再生医療学会総会  第11回日本再生医療学会総会

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    Event date: 2012.6

    Language:Japanese  

    Venue:神奈川  

    国内学会

  • 尾形 彰, 三井 薫, 小戝 健一郎   同定・単離技術を中心としたヒトES 細胞/iPS 細胞による再生医学の研究  

    第5回 桜ヶ丘地区基礎系研究発表会  第5回 桜ヶ丘地区基礎系研究発表会

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    Event date: 2012.1

    Language:Japanese  

    Venue:鹿児島  

    研究会

  • 高山明子、和田忠久、田上聖徳、王宇清、三井薫、武田泰生、山田勝士、小戝健一郎   革新的な癌治療薬を目指したオリジナルの癌特異的増殖型アデノウイルスの開発  

    第27回 日本薬学会九州支部大会  第27回 日本薬学会九州支部大会

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    Event date: 2010.12

    Language:Japanese  

    Venue:長崎  

    国内学会

  • Mitani K, Mitsui K, Suzuki K, Aizawa E, Kawase E, Suemori H, Nakatsuji N.   Gene targeting in human pluripotent stem cells with adeno-associated virus vectors  

    第16回 日本遺伝子治療学会学術集会  第16回 日本遺伝子治療学会学術集会

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    Event date: 2010.7

    Language:Japanese  

    Venue:栃木  

    国内学会

  • 三谷幸之介, 鈴木啓一郎, 三井薫, 相澤絵美   ヒト多能性幹細胞におけるウイルスベクターを用いた遺伝子ターゲッテッング  

    第9回日本再生医療学会総会  第9回日本再生医療学会総会

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    Event date: 2010.3

    Language:Japanese  

    Venue:広島  

    国内学会

  • Ko Mitani, Keiichiro Suzuki, Yuka Hirabayashi, Kaoru Mitsui, Emi Aizawa   Comparison of viral veetors for gene repair therapy of inherited disorders using human induced pluripotent stem cells   International conference

    第1回アジア先天代謝異常学会会議  第1回アジア先天代謝異常学会会議

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    Event date: 2010.3

    Language:English  

    Venue:福岡  

    国際学会

  • 山本 芳丈, 三井 薫, 小戝 健一郎, 宮脇 正一   骨芽細胞へのiPS細胞分化過程におけるES細胞と比較したMsx2の影響  

    日本矯正歯科学会大会プログラム・抄録集  2017.10  (公社)日本矯正歯科学会

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Research Projects

  • 心筋分化特異的miRNAによる心筋分化転換法の確立と心筋分化調節機構の解明

    Grant number:22K08105  2022.4 - 2025.3

    科学研究費助成  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Competitive

  • miRNAを用いた効率的な心筋ダイレクトリプログラミング法の確立と分子基盤の解明

    Grant number:19K08562  2019.4 - 2023.3

    科学研究費助成  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Competitive

  • ヒトES/iPS細胞由来心筋細胞の腫瘍化克服のための革新的な高純度単離技術の開発

    Grant number:24591099  2012.4 - 2016.3

    科学研究費助成  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Competitive

  • 野生型マウス由来体細胞からの誘導性多能性幹細胞(iPS細胞)の樹立

    Grant number:20790235  2008.4 - 2010.3

    科学研究費助成  若手研究(B)

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    Authorship:Principal investigator  Grant type:Competitive

  • ウイルスベクターを用いた相同組換えによるヒトES細胞の染色体操作法の開発と研究用モデル細胞の創製

    2006

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Competitive

 

Teaching Experience

  • 解剖学I

  • 最先端医療を創出するバイオ研究

  • 幹細胞と再生医学

    Institution:鹿児島大学

  • 医学生物学

  • 再生・移植学

  • 人体の構造と機能

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Social Activities

  • 韮崎高等学校SSH 鹿児島科学研修

    Role(s): Lecturer

    韮崎高等学校SSH  2016.2

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    Audience: High school students

    Type:Visiting lecture

  • 鹿児島実業高等学校 出前授業

    Role(s): Lecturer

    鹿児島実業高等学校  鹿児島実業高校  2015.9

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    Audience: High school students

    Type:Visiting lecture

    「幹細胞と再生医学」の講義

  • 宮崎第一中学高等学校 出前授業

    Role(s): Lecturer

    宮崎第一中学高等学校  宮崎第一中学高等学校  2015.7

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