Updated on 2024/04/11

写真a

 
SHIMIZU Keiichi
 
Organization
Research Field in Agriculture, Agriculture, Fisheries and Veterinary Medicine Area Faculty of Agriculture Department of Agriculture Associate Professor
Title
Associate Professor

Degree

  • 博士（農学） ( 1998.4   鹿児島大学 )

Research Interests

  • mutation breeding

  • plant genome instability

  • somaclonal variation

  • tissue culture

  • cell fusion

  • genetic transformation

Research Areas

  • Life Science / Applied molecular and cellular biology

  • Others / Others  / 花卉園芸学

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

  • Environmental Science/Agriculture Science / Horticultural science

Education

  • Kagoshima University

    - 1998.3

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    Country： Japan

  • University of Miyazaki

    1992.4 - 1994.3

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    Country： Japan

  • University of Miyazaki   Department of Agriculture

    1988.4 - 1992.3

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    Country： Japan

Research History

  • Kagoshima University   Associate Professor

    1998.8

  • Kagoshima University   Associate Professor

    1998.8

Professional Memberships

  • 日本育種学会

    2015.10

  • 園芸学会

    2015.10

  • Japanese Society of Plant Physiologists

    2014.1

Committee Memberships

  • 園芸学会   論文編集委員会  

    2017.4 - 2020.3   

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    Committee type：Other

  • 園芸学会   大会実行委員  

    2016.10 - 2018.10   

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    Committee type：Other

  • 日本植物生理学会   大会実行委員  

    2015.3 - 2017.3   

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    Committee type：Academic society

 

Papers

  • Yuka Takatori, Keiichi Shimizu, Daraluck Yauwapaksopon, Yu Nakamura, Hiroshi Oshima, Fumio Hashimoto .  Flavonoid 3',5'-Hydroxylase (F3'5'H) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] .  The Horticulture Journal91 ( 2 ) 229 - 239   2022.4Flavonoid 3',5'-Hydroxylase (F3'5'H) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]Reviewed International coauthorship

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    Authorship：Corresponding author   Language：English  

    DOI： https://doi.org/10.2503/hortj.UTD-320

    Other Link： https://www.jstage.jst.go.jp/article/hortj/91/2/91_UTD-320/_article/-char/ja

  • Atsushi Hoshino, Takayuki Mizuno, Keiichi Shimizu, Shoko Mori, Sachiko Fukada-Tanaka, Kazuhiko Furukawa, Kanako Ishiguro, Yoshikazu Tanaka, Shigeru Iida .  Generation of Yellow Flowers of the Japanese Morning Glory by Engineering Its Flavonoid Biosynthetic Pathway toward Aurones .  Plant and Cell Physiology28 ( 8 ) 1871 - 1879   2019.5Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press  

    Wild-type plants of the Japanese morning glory (Ipomoea nil) produce blue flowers that accumulate anthocyanin pigments, whereas its mutant cultivars show wide range flower color such as red, magenta and white. However, I. nil lacks yellow color varieties even though yellow flowers were curiously described in words and woodblocks printed in the 19th century. Such yellow flowers have been regarded as ‘phantom morning glories’, and their production has not been achieved despite efforts by breeders of I. nil. The chalcone isomerase (CHI) mutants (including line 54Y) bloom very pale yellow or cream-colored flowers conferred by the accumulation of 2′, 4′, 6′, 4-tetrahydoroxychalcone (THC) 2′-O-glucoside. To produce yellow phantom morning glories, we introduced two snapdragon (Antirrhinum majus) genes to the 54Y line by encoding aureusidin synthase (AmAS1) and chalcone 4′-O-glucosyltransferase (Am4′CGT), which are necessary for the accumulation of aureusidin 6-O-glucoside and yellow coloration in A. majus. The transgenic plants expressing both genes exhibit yellow flowers, a character sought for many years. The flower petals of the transgenic plants contained aureusidin 6-O-glucoside, as well as a reduced amount of THC 2′-O-glucoside. In addition, we identified a novel aurone compound, aureusidin 6-O-(6″-O-malonyl)-glucoside, in the yellow petals. A combination of the coexpression of AmAS1 and Am4′CGT and suppression of CHI is an effective strategy for generating yellow varieties in horticultural plants.

    DOI： 10.1093/pcp/pcz101

  • 「Yuka Takatori」「Keiichi Shimizu」「Jun Ogata」「Hiroki Endo」「Kanji Ishimaru」「Shigehisa Okamoto」 「Fumio Hashimoto」 .  Cloning of the Flavonoid 3'-Hydroxylase Gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3'H) and Complementation of an F3'H-deficient Mutant of Ipomoea nil (L.) Roth. by Heterologous Expression of EgF3'H .  The Horticulture Journal84 ( 2 ) 131 - 139   2015.4Reviewed

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Japanese Society for Horticultural Science (JSHS)  

    A full-length cDNA of a putative flavonoid 3'-hydroxylase (F3'H) gene encoding a key enzyme in the production of cyanidin was cloned from a lisianthus (Eustoma grandiflorum) petal. Lisianthus F3'H (EgF3'H) shares 75.1, 73.8, and 68.2% amino acid identity with Arabidopsis thaliana, Ipomoea nil, and Petunia hybrida, respectively. RT-PCR revealed that wild-type lisianthus flowers accumulated higher levels of F3'H mRNA during the early stages of development than in the late stages. The accumulated F3'H transcript levels in leaves were similar to those in flowers in the early stages of development. Overexpression of lisianthus F3'H cDNA altered flower color from red to blue in the I. nil cultivar ‘Violet’, which lacks a functional F3'H gene. In addition, the transgenic ‘Violet’ plants accumulated cyanidin and peonidin at similar levels to wild-type I. nil. Taking these findings together, this study demonstrates that EgF3'H functions as a flavonoid 3'-hydroxylase with a role in the synthesis of cyanidin and peonidin pigments.

    DOI： http://doi.org/10.2503/hortj.MI-029

  • Yuka Takatori, Yutaro Ichinomoto, Keiichi Shimizu, Fumio Hashimoto .  Inheritance of Hose-in-hose Flower Shape Origins in Wild-type Eustoma .  Horticultural research14 ( 4 ) 341 - 348   2015Inheritance of Hose-in-hose Flower Shape Origins in Wild-type EustomaReviewed

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：The Japanese Society for Horticultural Science (JSHS)  

    DOI： 10.2503/hrj.14.341

  • Kenichi Shibuya, Keiichi Shimizu, Tomoko Niki, Kazuo Ichimura .  Identification of a NAC transcription factor, EPHEMERAL1, that controls petal senescence in Japanese morning glory .  The plant jouranal79 ( 6 ) 1044 - 1051   2014.1Identification of a NAC transcription factor, EPHEMERAL1, that controls petal senescence in Japanese morning gloryReviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Shimizu K., Hashimoto, F. .  The Construction of Two New Activation Tagging Vectors, pKANAC2H and pKANAC2K. .  Mem. Fac. Agric. Kagoshima Univ.64   37 - 43   2014.1The Construction of Two New Activation Tagging Vectors, pKANAC2H and pKANAC2K.

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    Language：English   Publishing type：Research paper (other academic)  

  • Shimizu K., Hashimoto, F. .  A New Series of Activation Tagging Vectors, pKANACB, pKANACK and pKANACH. .  Mem. Fac. Agric. Kagoshima Univ.63   39 - 47   2013.3A New Series of Activation Tagging Vectors, pKANACB, pKANACK and pKANACH.

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Keiichi shomizu, Nanako Ohnishi, Noriyuki Morikawa, Ai Ishigami, Saeko Otake,Isselmou Ould Rabah, Yusuke Sakata, Fumio Hashimoto .  A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines ofLisianthus [Eustoma grandiflorum (Raf.) Shinn.] .  J. Japan. Soc. Hort. Sci. 80 ( 4 ) 434 - 442   2011.4A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines ofLisianthus [Eustoma grandiflorum (Raf.) Shinn.]Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • kenichi Shibuya, keiichi Shimizu, Tetsuya Yamada and Kazuo Ichimura .  Expression of autophagy-associated ATG8 genes during petal senescence in Japanese morning glory. .  Journal of Japanese Society Horticultural Science.80   89 - 95   2011.1Expression of autophagy-associated ATG8 genes during petal senescence in Japanese morning glory.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • kenichi Shibuya, Tetsuya Yamada, Tomoko Suzuki, keiichi Shimizu and kazuo Ichimura .  InPSR26, a putative membrane protein, regulates programmed cell death during petal senescence in Japanese morning glory. .  Plant Physiology. 149   816 - 824   2009.2InPSR26, a putative membrane protein, regulates programmed cell death during petal senescence in Japanese morning glory. Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Jian-Bin LI, Fumio HASHIMOTO, Keiichi SHIMIZU, Yusuke SAKATA .  Anthocyanins from Red Flowers of Camellia saluenensis LINDL. .  journal of the japanese society for horticulturel science77 ( 1 ) 75 - 79   2008.1Anthocyanins from Red Flowers of Camellia saluenensis LINDL. Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • 李 家華、石黒悦爾、根角厚司、石川大太郎、清水圭一、坂田祐介、橋本文雄 .  肥料の違いが茶ポリフェノール含量に与える影響 .  農業生産技術管理学会14 ( 2 ) 87 - 92   2007.12肥料の違いが茶ポリフェノール含量に与える影響Reviewed

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Jian-Bin LI, Fumio HASHIMOTO, Keiichi SHIMIZU, Yusuke SAKATA .  Anthocyanins from Red Flowers of Camellia reticulata LINDL. .  Bioscience, Biotechnology, and Biochemistry 71 ( 11 ) 2833 - 2836   2007.1Anthocyanins from Red Flowers of Camellia reticulata LINDL.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Keiichi Shimizu, Tetsuya Tokumura. Fumio Hashimoto, Yusuke sakata .  In Vitro Propagation of Sterile Mutant Strains in Japanese Morning Glory by Sub-culturing Embryoids Derived from an Immature Embryo. .  Journal of. Japanese . Society . Horticultural. Science74（4） ( 4 ) 311 - 317   2005.11In Vitro Propagation of Sterile Mutant Strains in Japanese Morning Glory by Sub-culturing Embryoids Derived from an Immature Embryo.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • keiichi Shimizu, T. Yogai, fumio Hashimoto and Yusuke Sakata .  Plant regeneration from suspension culture cell of Delphinium. .  Journal of. Japanese . Society . Horticultural. Science73（5） ( 5 ) 435 - 440   2004.9Plant regeneration from suspension culture cell of Delphinium.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Fumio Hashimoto, F., A. F. M. J，Uddin, Keiichi Shimizu, Yusuke Sakata .  Multiple allelism in Flavonoid hydroxylation in Eustoma grandiflorum (Raf.) Shinn. Flower .  Journal of. Japanese . Society . Horticultural. Science73（3） ( 3 ) 235 - 240   2004.5Multiple allelism in Flavonoid hydroxylation in Eustoma grandiflorum (Raf.) Shinn. FlowerReviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

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Presentations

  • 潟山 祐樹・ブスング コンスタンティン・田浦 悟・豊元 大希・穴井 豊昭・鈴木 章弘・内海 俊樹・志水 勝好・岡本 繁久・清水 圭一・一谷 勝之   Kas-II 遺伝子の非同義置換がもたらすイネ白葉枯病抵抗性   International coauthorship

    日本育種学会  2020.10  日本育種学会

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    Event date： 2020.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン開催   Country：Japan  

  • 一谷勝之・清水圭一・橋本文雄・内海俊樹・玉置尚徳・山本雅史・吉田理一郎・岡本繁久・二神泰基・尾上昌平・久保山勉・田浦悟   イオンビーム照射によるHwa1-1, Hwa2-1が引き起こすイネ雑種弱勢克服の試みと突然変異キメラ研究の可能性  

    日本育種学会　第14回九州育種談話会  2019.11  日本育種学会

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    Event date： 2019.11

    Language：Japanese  

    Venue：東海大学熊本キャンパス   Country：Japan  

  • 江上大貴・柴田有里佳・針原彩乃・小畑舞・清水圭一・橋本文雄   トルコギキョウの斑入り花系統で見つかったDNA型トランスポゾンの解析  

    園芸学会令和元年度秋季大会  2019.9  園芸学会

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    Event date： 2019.9

    Venue：島根大学   Country：Japan  

  • 柴田有里佳・江上大貴・針原彩乃・中村謙孝・清水圭一・橋本文雄   トルコギキョウの斑入り花形質の遺伝とトランスポゾンの関係  

    園芸学会令和元年度秋季大会  2019.9  園芸学会

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    Event date： 2019.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：島根大学   Country：Japan  

  • 岡本歩・星野敦・福多賢太郎・野口英樹・田中啓介・乗越亮・清水圭一・仁田坂英二   アサガオの葉における翼片形成に関わる遺伝子の解析2  

    第10回アサガオ研究集会  2019.3  アサガオ研究集会

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    Event date： 2019.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：茨城大学   Country：Japan  

  • 江上大貴・小畑舞・針原彩乃・福山達也・髙取由佳・清水圭一・橋本文雄   トルコギキョウの花弁の斑入りに関する研究  

    植物色素研究会　第29回集会  2017.10  植物色素研究会

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    Event date： 2017.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：崇城大学   Country：Japan  

  • 髙取由佳・清水圭一・橋本文雄   Anthocyanidin synthase (ANS) 遺伝子の多型がトルコギキョウ花弁のアントシアニン色素の蓄積に及ぼす影響  

    園芸学会平成29年度秋季大会  2017.9  園芸学会

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    Event date： 2017.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：北海道酪農大学   Country：Japan  

  • 安永智希・加藤文俊・鮫島理沙・田代桃子・江上大貴・清水圭一・髙取由佳・草留大陸・橋本文雄   近年のトルコギキョウ品種にみられる新奇な花冠形質の遺伝について  

    園芸学会平成29年度秋季大会  2017.9  園芸学会

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    Event date： 2017.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：北海道酪農大学   Country：Japan  

  • Keiichi Shimizu, Satoko Tabuchi Fumio Hashimoto   Activation tagging in ornamental tomato `Micro-Tom' reviled that over expression of AP2 like gene alters flower size, flowering time and plant height.  

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    Event date： 2016.9

    Language：Japanese   Presentation type：Poster presentation  

  • Takatori Yuka, Shimizu Keiichi, Hashimoto Fumio   Inheritance of yellow colored flower in Eustoma grandiflorum (Raf.) Shinn.  

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    Event date： 2016.9

    Language：Japanese   Presentation type：Poster presentation  

  • Takatori Yuka, Ichinomoto Yutaro, Shimizu keiichi, Hashimoto Fumio   Inheritance of hose-in-hose flower shape origins in wild type Eustoma.  

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    Event date： 2015.9

    Language：Japanese   Presentation type：Poster presentation  

  • 髙取 由佳, 米澤 徹, 前島 理奈, 清水 圭一, 橋本 文雄   Flavonoid 3’,5’-hydroxylase (F3’5’H) 遺伝子の多型がトルコギキョウ花弁のアントシアニジン組成に及ぼす影響  

    園芸学会  園芸学会

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    Event date： 2014.9

    Language：Japanese  

    Venue：佐賀  

    国内学会

  • 髙取由佳・清水圭一・福山達也・橋本文雄   トルコギキョウEustoma grandiflorum（Raf.）shinn.品種‘スピカロマン’の斑入りは，F3H遺伝子へのトランスポゾン挿入により引き起こされる  

    園芸学会  園芸学会

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    Event date： 2014.3

    Language：Japanese  

    Venue：茨城  

    国内学会

  • 清水圭一、平田千裕、安丸公貴、橋本文雄   観賞用トマト‘マイクロトム’(Solanum lycopersicum cv. Micro-Tom)の形質転換に影響を及ぼす諸要因の検討  

    園芸学会  園芸学会

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    Event date： 2013.9

    Language：Japanese  

    Venue：岩手  

    国内学会

  • 清水圭一、久保田翔平、橋本文雄   観賞用トマト‘マイクロトム’(Solanum lycopersicum cv. Micro-Tom)におけるactivation taggingベクターを使用した形質転換からの変異体の作出  

    園芸学会  園芸学会

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    Event date： 2012.9

    Language：Japanese  

    Venue：福井  

    国内学会

  • Keiichi Shimizu, Miho Kato, Shyouhei Kubota , Fumio Hashimoto and Yusuke Sakata   Regeneration of mutant plants from a transformation using activation tagging vector in model cultivar of tomato ‘Micro-Tom’   International conference

    SOL and ICuGI 2011 (国際ナス科ウリ科合同国際会議)  SOL and ICuGI 2011 (国際ナス科ウリ科合同国際会議)

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    Event date： 2011.11

    Language：English  

    Venue：神戸  

    国際学会

  • 大竹沙永子、清水圭一、緒方 潤、遠藤大輝、福田良絵、ウレド ラバ イセルモ、坂田祐介、橋本文雄   トルコギキョウのフラボノイド3’-水酸化酵素遺伝子のクローニングとアサガオでの異種性発現によるF3’H欠損変異の相補  

    園芸学会  園芸学会

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    Event date： 2010.9

    Language：Japanese  

    Venue：大分  

    国内学会

  • 大竹沙永子、清水圭一、大西奈々子、森川典幸、石神 愛、イセルモ ウレド ラバ、坂田祐介、橋本文雄   トルコギキョウの白色花品種で見出されたアントシアニジン合成酵素の欠失変異  

    園芸学会  園芸学会

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    Event date： 2009.9

    Language：Japanese  

    Venue：秋田  

    国内学会

  • ダララク ヤウワパクソポン・清水圭一・橋本文雄・緒方潤・イセルモ ウレド ラバ・坂田祐介   トルコギキョウの花色の多様化にはフラボノイド3’,5’-水酸化酵素遺伝子へのレトロトランスポゾンの挿入が関係していた  

    園芸学会  園芸学会

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    Event date： 2007.9

    Language：Japanese  

    Venue：香川  

    国内学会

  • Yauwapaksopon Daraluck, Keiichi Shimizu, Fumio Hashimoto, Jun Ogata, Yusuke Sakata   Insertion of a novel retrotransposon into flavonoid 3',5'-hydroxylase gene in lisianthus [Eustoma grandiflorum (Raf.) shinn.]   International conference

    アメリカ園芸学会  アメリカ園芸学会

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    Event date： 2007.7

    Language：English  

    Venue：米国  

    国際学会

  • 橋本文雄、福田祥子、松尾寛子、田中見佳、清水圭一、樗木直也、石黒悦爾、坂田祐介   青色系デルフィニウムの花色素と花色との数理的モデル解析  

    園芸学会  園芸学会

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    Event date： 2006.9

    Language：Japanese  

    Venue：長崎  

    国内学会

  • 橋本文雄、福田祥子、松尾(前田)寛子、田中見佳、清水圭一、坂田祐介   青色系デルフィニウムの新規花色素を含む交配種の作出  

    園芸学会  園芸学会

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    Event date： 2005.10

    Language：Japanese  

    Venue：宮城  

    国内学会

  • 橋本文雄、清水圭一、坂田祐介   秋咲き性ツツジの育種と花弁アントシアニジン色素の遺伝  

    園芸学会  園芸学会

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    Event date： 2004.9

    Language：Japanese  

    Venue：静岡  

    国内学会

  • 向坂佳代子、清水圭一、橋本文雄、坂田祐介   アサガオ（Ipomoea nil)懸濁培養カルスを利用した効率的な形質転換  

    園芸学会  園芸学会

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    Event date： 2004.9

    Language：Japanese  

    Venue：静岡  

    国内学会

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Awards

  • 園芸学会年間優秀論文賞

    2023.3   園芸学会  

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    Award type：Honored in official journal of a scientific society, scientific journal 

    トルコギキョウ［Eustoma grandiflorum (Raf.) Shinn.］は，アメリカ合衆国南部原産のリンドウ科の草本である．本種は戦後の日本で育種が進み，高い草丈，豊富な花色と花型，花持ちの良さ等が評価され我が国の主要な花卉の1つになっている．トルコギキョウの野生種は，デルフィニジン系アントシアニンを主要色素とする紫色の花を咲かせるが，現在では赤，白，ピンク，薄緑，薄黄，茶，藤色等の様々な花色を持った品種が作出されている．もし，これらの変異の原因となっているDNA配列がわかれば，DNA鑑定によって開花前にそれらの形質を知ることができるようになり，効率的に品種改良を行うことが可能になる．しかしながら，トルコギキョウでは花色の変異とゲノム上のDNAの変異を関連付ける研究はほとんど進んでいないのが現状であった．

    本論文では，このような花色変異の原因となるDNA配列を明らかにすることを目的に，デルフィニジン系アントシアニン生合成の鍵酵素である3′, 5′-水酸化酵素遺伝子（F3′5′H）の調査を行った．トルコギキョウの色素組成の異なる3系統，すなわち，デルフィニジンが花弁の主要色素となる紫色花系統，シアニジンが主要色素となる藤色花系統及びペラルゴニジンが主要色素となるピンク色花系統において，F3'5'H遺伝子をゲノムPCRによって増幅し電気泳動した．その結果，3系統とも異なるバンドパターンが検出された．さらに，遺伝学的解析からそれらのバンドパターンは同じ遺伝子座の変異による多型であること，さらに，この多型が花色の変異と連鎖していることが明らかになった．また，塩基配列を調べたところ，ペラルゴニジンが主要色素となる系統の多型は，レトロトランスポゾンが挿入によるものであり，この挿入によって，F3′5′H遺伝子に変異が起こり，ペラルゴニジン主要型の表現型になったと考えられる．以上の結果，本研究によって見出されたトルコギキョウのF3′5′H遺伝子の多型は，花弁の主要色素を開花前に予測するためのDNAマーカーとして利用可能であり，効率的な花色の育種につながることが期待される．

  • JSHS Excellent Paper Award

    2015.3   Japanese Society for Horticultural Science   Cloning of the Flavonoid 3'-Hydroxylase Gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3'H) and Complementation of an F3'H-deficient Mutant of Ipomoea nil (L.) Roth. by Heterologous Expression of EgF3'H

    Yuka Takatori, Keiichi Shimizu, Jun Ogata, Hiroki Endo, Kanji Ishimaru, Shigehisa Okamoto, Fumio Hashimoto

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    Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

    A full-length cDNA of a putative flavonoid 3'-hydroxylase (F3'H) gene encoding a key enzyme in the production of cyanidin was cloned from a lisianthus (Eustoma grandiflorum) petal. Lisianthus F3'H (EgF3'H) shares 75.1, 73.8, and 68.2% amino acid identity with Arabidopsis thaliana, Ipomoea nil, and Petunia hybrida, respectively. RT-PCR revealed that wild-type lisianthus flowers accumulated higher levels of F3'H mRNA during the early stages of development than in the late stages. The accumulated F3'H transcript levels in leaves were similar to those in flowers in the early stages of development. Overexpression of lisianthus F3'H cDNA altered flower color from red to blue in the I. nil cultivar `Violet', which lacks a functional F3'H gene. In addition, the transgenic `Violet' plants accumulated cyanidin and peonidin at similar levels to wild-type I. nil. Taking these findings together, this study demonstrates that EgF3'H functions as a flavonoid 3'-hydroxylase with a role in the synthesis of cyanidin and peonidin pigments.

Research Projects

  • トルコギキョウの斑入り花で見つかったトランスポゾンの解析と利用

    2016.4 - 2019.3

    科学研究費補助金  基盤研究(C)

    清水圭一、橋本　文雄

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    トルコギキョウの斑入り花で見つかったトランスポゾンの分布と転移活性を調査し、本種における突然変異育種のツールとして利用可能かどうかを検討する。

 

Media Coverage

  • 幻の黄色いアサガオ開花 Newspaper, magazine

    朝日新聞鹿児島支局  朝日新聞鹿児島版  朝日新聞鹿児島25ページ  2014.11

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    Author：Other 

  • 24時間咲くアサガオ Newspaper, magazine

    南日本新聞社  南日本新聞  29ページ  2014.7