研究者詳細 - 榊間　春利

Matsuzaki R., Matsuoka T., Nakanishi K., Tani A., Kakimoto S., Kato Y., Kawatani T., Nakagawa S., Baba Y., Kobayashi M., Takihara T., Sakakima H. . Effects of green tea catechins and exercise on age-related muscle atrophy and satellite cell functions in a mouse model of sarcopenia . Experimental Gerontology202 112720 2025年4月

記述言語：日本語出版者・発行元：Experimental Gerontology

Sarcopenia negatively affects the quality of life and health of older individuals. Physical exercise is a standard treatment for sarcopenia. Recently, the potential benefits of green tea catechins (GTCs) in sarcopenia have gained considerable attention. In this study, we investigated the effects of a combination of GTCs and physical exercise on the symptoms and pathologies of sarcopenia using male senescence-accelerated mouse prone 8 (SAMP8). These mice were divided into four groups: control, GTCs, exercise (Ex), and GTCs + Ex. GTC-fed mice were fed a diet containing 0.33 % GTCs. The mice were subjected to exercise training (voluntary wheel running) for 12 weeks, from 5 to 8 months of age, and grip strength and gastrocnemius muscle alterations were investigated. SAMP8 mice exhibited symptoms and pathologies of sarcopenia, including loss of muscle mass, decreased grip strength, reduced mitochondrial capacity, increased oxidative stress, reduced number of satellite cells, and an increased ratio of 5-bromo-2′-deoxyuridine (BrdU)-positive nuclei located within the muscle cells in the aged muscle. The GTCs and/or Ex groups showed improved symptoms and pathologies of sarcopenia. In addition, the GTCs + Ex group exhibited enhanced mitochondrial capacity, myogenic differentiation, and maturation in aged skeletal muscle than that observed in the GTCs group. Our findings suggested that GTCs and/or Ex are effective in ameliorating several age-related changes in muscle morphology and function. Notably, GTCs intake, together with habitual exercise, may enhance the beneficial effects on the symptoms and pathologies of sarcopenia in aged muscle.

DOI： 10.1016/j.exger.2025.112720

榊間春利 . 脳を基礎から理解する：運動による脳保護効果 . 老年歯科医学39 ( 4 ) 196 - 201 2025年

松岡輝樹, 松崎凌真, 中西和毅, 榊間春利 . 運動器疾患を有した地域在住高齢者におけるTimed up and go testを用いた日中身体活動パターンの分析 . 理学療法かごしま4 ( 0 ) 1 - 8 2025年

記述言語：日本語出版者・発行元：公益社団法人鹿児島県理学療法士協会

運動器疾患を有した地域在住高齢者の在宅での身体活動量を評価することは，疾患の進行に伴う身体機能低下を予防するために重要である。本研究の目的は歩行能力の評価指標であるTimed up and go test（以下，TUG）を用いて，運動器疾患を有した地域在住高齢者の日中の身体活動量や身体活動パターンの特徴を把握することができるか調べることである。対象は運動器疾患を有して外来リハビリテーションに通院する65歳以上の地域在住高齢者73名（男性16名，女性57名，平均年齢81.2歳）とした。対象者のTUGの平均値（12.9 秒）を基準に13秒未満を歩行能力良好群（46名），13秒以上を歩行能力不良群（2名）に分類した。身体活動量の計測にはオムロン活動量計を用い，1週間当たりの身体活動量（歩数，歩行活動量，生活活動量）を計測した。全ての身体活動量は，歩行能力良好群と比較して不良群で有意に減少していた。また，両群ともに生活活動量と比較して歩行活動量が有意に低下していた。良好群の日中の身体活動パターンは，午前と午後にピークを迎える二峰性の変化を示した。しかし，不良群の歩行活動パターンは明らかな二峰性がみられず，1日を通して低下していた。重回帰分析の結果，TUGに影響する因子として日中の歩数と生活活動量が抽出された。本研究はTUGを用いて，運動器疾患を有した地域在住高齢者の在宅での身体活動量や身体活動パターンの特徴を推察できる可能性を示唆した。

DOI： 10.57357/ptkagoshima.4.0_1

Nishiwaki M., Takada S., Otsuka S., Makizako H., Sakakima H., Shiomi N., Ooba S., Matsumoto N., Kikuchi K. . Effects of agricultural or gardening physical activity on cardiovascular disease and dementia-related markers via arterial stiffness, cognitive function, and cerebral white matter status: results from cross-sectional and interventional studies . Frontiers in Public Health13 1509528 2025年

記述言語：日本語出版者・発行元：Frontiers in Public Health

Background: Agricultural or gardening physical activity (AGPA) offers potential as a simple strategy to improve cardiovascular disease and dementia-related markers; however, the actual preventive effects remain unclear. Our objective was to investigate AGPA regarding related markers of cardiovascular disease and dementia using cross-sectional and interventional approaches. Methods and results: In Study 1, community-based older individuals were assessed, and 30 individuals who performed AGPA (AG group) and 30 1:1 age-, sex-, and objective activity-matched controls were cross-sectionally compared (mean age, 75 ± 6 y). Arterial stiffness (pulse wave velocity) was lower and hand-finger dexterity (pegboard test) was higher in the AG vs. control groups. The interventional results of Study 2 (secondary analysis of a randomized controlled trial) confirmed that consistent AGPA might regress white matter hyperintensities in older individuals. Conclusion: Our findings suggest that consistent AGPA perse may improve cardiovascular disease and dementia-related markers in older healthy individuals via arterial stiffness, cognitive function, and cerebral white matter status. This information could have major implications for integrated strategies for lifelong health. Secondary analysis of clinical trials (study 2): Study for Decreasing Depressive Symptoms and Increasing Memory Performance in the older adults (Trial registration number UMIN000018547, Date of registration 2015/08/07), URL: https://center6.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000021462.

DOI： 10.3389/fpubh.2025.1509528

Sumizono M, Yoshizato Y, Imai T, Tani A, Nakanishi K, Nojima N, Kakimoto S, Sakakima H . Effects of Pain Relief Through Minimal Exercise Intervention in a Rat Model of Neuropathic Pain. . Cureus16 ( 6 ) e62897 2024年6月

Otsuka S., Matsuzaki R., Kakimoto S., Tachibe Y., Kawatani T., Takada S., Tani A., Nakanishi K., Matsuoka T., Kato Y., Inadome M., Nojima N., Sakakima H., Mizuno K., Matsubara Y., Maruyama I. . Ninjin'yoeito reduces fatigue-like conditions by alleviating inflammation of the brain and skeletal muscles in aging mice . PLoS ONE19 ( 5 May ) e0303833 2024年5月

記述言語：日本語出版者・発行元：PLoS ONE

Fatigue can lead to several health issues and is particularly prevalent among elderly individuals with chronic inflammatory conditions. Ninjin'yoeito, a traditional Japanese herbal medicine, is used to address fatigue and malaise, anorexia, and anemia. This study aimed to examine whether relieving inflammation in the brain and skeletal muscle of senescenceaccelerated mice prone 8 (SAMP8) could reduce fatigue-like conditions associated with aging. First, SAMP8 mice were divided into two groups, with and without ninjin'yoeito treatment. The ninjin'yoeito-treated group received a diet containing 3% ninjin'yoeito for a period of 4 months starting at 3 months of age. At 7 months of age, all mice underwent motor function, treadmill fatigue, and behavioral tests. They were then euthanized and the skeletal muscle weight, muscle cross-sectional area, and concentration of interleukin (IL)-1β and IL- 1 receptor antagonist (IL-1RA) in both the brain and skeletal muscle were measured. The results showed that the ninjin'yoeito-treated group had higher motor function and spontaneous locomotor activity than the untreated group dand ran for significantly longer in the treadmill fatigue test. Moreover, larger muscle cross-sectional area, lower IL-1β concentrations, and higher IL-1RA concentrations were observed in both the brain and skeletal muscle tissues of the ninjin'yoeito-treated group than in the untreated group. The results suggest that ninjin'yoeito improves age-related inflammatory conditions in both the central and peripheral tissues and reduces fatigue.

DOI： 10.1371/journal.pone.0303833

Otsuka S., Kikuchi K., Takeshita Y., Takada S., Tani A., Sakakima H., Maruyama I., Makizako H. . Relationship between physical activity and cerebral white matter hyperintensity volumes in older adults with depressive symptoms and mild memory impairment: a cross-sectional study . Frontiers in Aging Neuroscience16 1337397 2024年

記述言語：日本語出版者・発行元：Frontiers in Aging Neuroscience

Introduction: Cerebral white matter hyperintensities (WMHs) are commonly found in the aging brain and have been implicated in the initiation and severity of many central nervous system diseases. Furthermore, an increased WMH volume indicates reduced brain health in older adults. This study investigated the association between WMH volume and physical activity in older adults with depressive symptoms (DS) and mild memory impairment (MMI). Factors associated with the WMH volume were also investigated. Methods: A total of 57 individuals aged over 65 years with DS and MMI were included in this study. The participants underwent magnetic resonance imaging to quantify WMH volumes. After WMH volume was accumulated, normalized to the total intracranial volume (TIV), the percentage of WMH volume was calculated. In addition, all participants wore a triaxial accelerometer for 2 weeks, and the average daily physical activity and number of steps were measured. The levels of blood biomarkers including cortisol, interleukin-6 (IL-6), brain-derived insulin-like growth factor-1, and brain-derived neurotrophic factor were measured. Motor and cognitive functions were also assessed. Results: Faster maximum walking speed and longer time spent engaged in moderate physical activity were associated with a smaller percent of WMH volume, whereas higher serum IL-6 levels were associated with a larger percent of WMH volume. The number of steps per day, time spent engaged in low levels of physical activity, cognitive function, and all other measured biomarkers were not significantly associated with percent of WMH volume. Discussion: Higher blood inflammatory cytokine levels, shorter duration of moderate physical activity, and lower maximum walking speed were associated with a higher percent of WMH volume. Our results provide useful information for maintaining brain health in older adults at a high risk of developing dementia and may contribute to the development of preventive medicine for brain health.

DOI： 10.3389/fnagi.2024.1337397

Natsume K., Yoshida A., Sakakima H., Yonezawa H., Kawamura K., Akihiro S., Hanaya R., Shimodozono M. . Age-independent benefits of postoperative rehabilitation during chemoradiotherapy on functional outcomes and survival in patients with glioblastoma . Journal of Neuro-Oncology170 ( 1 ) 129 - 137 2024年

記述言語：日本語出版者・発行元：Journal of Neuro-Oncology

Purpose: To investigate the impact of early and continuous postoperative inpatient rehabilitation during chemoradiotherapy on functional outcomes and overall survival (OS) in patients with glioblastoma (GBM), particularly in different age groups. Methods: This retrospective cohort study at a university hospital (2011–2016) included 75 of 119 consecutive patients newly diagnosed with GBM who underwent standardized treatment and postoperative rehabilitation. Patients were divided into older (≥ 65 years, n = 45) and younger (< 65 years, n = 30) groups, engaging in a 50-day rehabilitation program. We assessed rehabilitation progress, Barthel Index (BI), Brunnstrom Recovery Stage (BRS), adverse events, and OS. BI at discharge and survival were analyzed using multivariate and Cox regression models, respectively. Results: The mean age was 72.5 ± 6.3 and 52.4 ± 7.8 years in the older and younger groups, respectively. Both groups demonstrated significant improvements in BI and BRS. Despite more adverse events in the older group, no significant difference existed in median OS (older group: 18.7 months vs. younger group: 18.3 months, p = 0.87). Early walking training, reduced fatigue during chemoradiotherapy, and high Karnofsky Performance Status at admission significantly impacted the BI at discharge. Cox regression analysis identified the BI at discharge as a significant predictor of survival (hazard ratio [HR] 0.98, 95% confidence interval [CI] 0.97–0.99, p = 0.008). Conclusion: Integrated rehabilitation improves functional outcomes, and enhanced ADL at discharge is associated with improved survival outcomes in patients with GBM, regardless of age. This highlights the need for personalized rehabilitation in treatment protocols. Further prospective studies are warranted to confirm these findings.

DOI： 10.1007/s11060-024-04785-1

Otsuka S., Itashiki Y., Tani A., Matsuoka T., Takada S., Matsuzaki R., Nakanishi K., Norimatsu K., Tachibe Y., Kitazato R., Nojima N., Kakimoto S., Kikuchi K., Maruyama I., Sakakima H. . Effects of different remote ischemia perconditioning methods on cerebral infarct volume and neurological impairment in rats . Scientific Reports13 ( 1 ) 2158 2023年12月

記述言語：日本語出版者・発行元：Scientific Reports

Remote ischemic perconditioning (RIPerC) is a novel neuroprotective method against cerebral infarction that has shown efficacy in animal studies but has not been consistently neuroprotective in clinical trials. We focused on the temporal regulation of ischemia–reperfusion by RIPerC to establish an optimal method for RIPerC. Rats were assigned to four groups: 10 min ischemia, 5 min reperfusion; 10 min ischemia, 10 min reperfusion; 5 min ischemia, 10 min reperfusion; and no RIPerC. RIPerC interventions were performed during ischemic stroke, which was induced by a 60-min left middle cerebral artery occlusion. Infarct volume, sensorimotor function, neurological deficits, and cellular expressions of brain-derived neurotrophic factor (BDNF), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and caspase 3 were evaluated 48 h after the induction of ischemia. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) was also performed. RIPerC of 10 min ischemia/10 min reperfusion, and 5 min ischemia/10 min reperfusion decreased infarct volume, improved sensorimotor function, decreased Bax, caspase 3, and TUNEL-positive cells, and increased BDNF and Bcl-2 expressions. Our findings suggest RIPerC with a reperfusion time of approximately 10 min exerts its neuroprotective effects via an anti-apoptotic mechanism. This study provides important preliminary data to establish more effective RIPerC interventions.

DOI： 10.1038/s41598-023-29475-2

Norimatsu K., Nakanishi K., Ijuin T., Otsuka S., Takada S., Tani A., Matsuzaki R., Matsuoka T., Sakakima H. . Effects of low-intensity exercise on spontaneously developed knee osteoarthritis in male senescence-accelerated mouse prone 8 . Arthritis Research and Therapy25 ( 1 ) 168 2023年12月

記述言語：日本語出版者・発行元：Arthritis Research and Therapy

Background: Osteoarthritis (OA) is a degenerative joint disease associated with aging, which often leads to joint stiffness and disability. Exercise is one of the most important non-pharmacological treatments and is prescribed as an indispensable treatment for OA. However, whether physical exercise is beneficial for preventing the progression of OA symptoms with age is poorly understood. We investigated the effects of exercise on spontaneously developed knee OA using male senescence-accelerated mouse prone 8 (SAMP8). Methods: To examine age-related changes in the knee joints of SAMP8, knee articular cartilage changes, synovitis, knee joint flexion and extension angles, swelling, walking ability, and quadriceps muscle atrophy were analyzed at 3, 5, 7, and 9 months. SAMP8 were required to run at a speed of 10 m/min for 15 min/day from 7 to 9 months of age. The knee joint pathologies and symptoms of exercising and non-exercising mice were compared by histological, immunohistochemical, and morphometrical analyses. Results: The mice presented with various histological changes, including cartilage destruction, osteocyte formation, synovitis, declined joint angles, and swelling. Notably, medial and posterior cartilage destruction was more severe than that of the lateral and anterior cartilage. Knee joint angles were significantly correlated with the histological scores (modified Mankin and OARSI, osteophyte formation and synovial lining cell layer). Exercise did not attenuate cartilage degeneration in the medial and posterior tibial plateau, although the articular cartilage of the anterior and lateral tibial plateau and its histological scores was remained and significantly improved, respectively, by exercise. Exercise suppressed the age-related decline of collagen type II-positive areas in the remaining articular cartilage and improved the OA symptoms. Exercise reduced the expression of monocyte chemoattractant protein (MCP)-1 and tumor necrosis factor (TNF)-α positive macrophages in the synovium. Conclusion: This study revealed that SAMP8 developed spontaneous knee OA with age, which resembled the disease symptoms in humans. Low-intensity exercise temporarily alleviated degeneration of the remaining cartilage, synovitis, and age-related decreases in knee flexion angle, stride length, and muscle atrophy in SAMP8. However, exercise during OA progression with age may cause mechanical stress that could be both beneficial and detrimental to joint health.

DOI： 10.1186/s13075-023-03162-z

則松貢輔, 中西和毅, 柿元翔吾, 野島菜央, 谷明, 松崎凌真, 松岡輝樹, 稲留真輝, 加藤夕貴, 立部勇汰, 高田聖也, 大塚章太郎, 角園恵, 榊間春利 . トレッドミル運動と水泳が老化促進マウスの自然発症型変形性膝関節症に及ぼす影響 . 基礎理学療法学26 ( 1 ) 1 - 10 2023年10月

記述言語：日本語出版者・発行元：(一社)日本基礎理学療法学会

本研究は自然発症型変形性膝関節症(以下,膝OA)を呈する老化促進マウス(SAMP8)を用いて,トレッドミル運動と水泳がOA病態,関節可動域,横径,歩容に及ぼす影響を調べ,運動効果の違いを明らかにすることを目的とした。マウスを非運動群,トレッドミル運動群,水泳群(各6匹)に無作為に分類した。トレッドミル運動は速度10～12m/min,水泳は水温37～38℃で,15分/日,週5日,6週間実施した。非運動群と比較して,両運動群は膝関節可動域や歩幅の改善を示した。さらにII型コラーゲン陽性軟骨細胞の増加,MMP-13陽性軟骨細胞の減少,滑膜のTNF-α陽性細胞の減少を認めた。特に水泳群の歩幅は有意な改善を認め,軟骨恒常性維持に重要なタンパク質であるII型コラーゲン陽性軟骨細胞は有意に増加していた。本研究は,運動が加齢による軟骨変性を緩和し,関節可動域や歩容を改善させることを示した。また,水泳が膝OA軟骨の恒常性維持や歩容改善に有益であることを示唆した。(著者抄録)

Nakanishi K, Norimatsu K, Tani A, Matsuoka T, Matsuzaki R, Kakimoto S, Nojima N, Tachibe Y, Kato Y, Inadome M, Kitazato R, Otsuka S, Takada S, Sumizono M, Sakakima H . Effects of early exercise intervention and exercise cessation on neuronal loss and neuroinflammation in a senescence-accelerated mouse prone 8. . Neuroscience letters808 137297 2023年5月

記述言語：英語出版者・発行元：Neuroscience Letters

Physical exercise is beneficial for preventing Alzheimer's disease (AD) and cognitive decline through several mechanisms, including suppression of neuroinflammation and neuronal loss in the hippocampus. Despite these exercise-induced benefits in AD pathology, less attention has been paid to the importance of maintaining exercise and the consequences of detraining. This study aimed to investigate the effects of early exercise intervention and detraining on age-related cognitive decline and its protective mechanisms using senescence-accelerated mouse prone 8 (SAMP8). These mice were divided to four groups: no-exercise (No-Ex, n = 9), 4 months (4 M)-detraining (n = 11), 2 months (2 M)-detraining (n = 11), and long-term exercise (LT-Ex, n = 13). Age-related cognitive decline was prevented in the LT-Ex group compared with the No-Ex group through the suppression of neuronal loss, enhanced brain-derived neurotrophic factor (BDNF), and inhibition of neuroinflammation corresponding to reduced M1 and increased M2 microglia in the hippocampus. No significant differences were observed in cognitive function between the detraining and No-Ex groups. However, the 2 M−detraining group showed increased BDNF positive area in the CA1 region and the enhancement of anti-inflammatory M2 phenotype microglia. In contrast, no statistically beneficial exercise-induced changes in the hippocampus were observed in the 4 M−detrainig group. These results showed that early exercise intervention prevented age-related cognitive deficits in AD progression by suppressing neuronal loss and neuroinflammation in the hippocampus. Exercise-induced benefits, including the anti-inflammation in the hippocampus, may be retained after exercise cessation, even if exercise-induced beneficial effects decline in a time-dependent manner.

DOI： 10.1016/j.neulet.2023.137297

宮﨑啓文, 有馬由貴, 竹下晃, 榊間春利 . Trunk Impairment Scaleを用いた痙直型脳性麻痺児の体幹機能と日常生活動作能力の関係性 . 小児理学療法学1 ( Supplement_1 ) 59 - 59 2023年3月

Tani A., Sakakima H., Otsuka S., Mizuno K., Nakanishi K., Norimatsu K., Takada S., Matsuoka T., Matsuzaki R., Nakakogawa T., Maruyama I. . Stimulation of functional recovery via neurorepair mechanisms by the traditional Japanese Kampo medicine, Ninjin'yoeito, and physical exercise in a rat ischemic stroke model . Journal of Ethnopharmacology302 ( Pt B ) 115927 2023年2月

記述言語：日本語出版者・発行元：Journal of Ethnopharmacology

Ethnopharmacological relevance: Ninjin'yoeito (NYT), a traditional Japanese Kampo medicine consisting of 12 herbs, has been reported to improve cognitive dysfunction, depression, and neurological recovery in patients with neurovascular diseases such as Alzheimer's disease and stroke. Several studies have reported that the NYT components exert neurotrophic, neurogenic, and neuroprotective effects. In addition, exercise enhances neuroprotection and functional recovery after stroke. Rehabilitative exercises and pharmacological agents induce neurophysiological plasticity, leading to functional recovery in stroke patients. These reports indicate that NYT treatment and exercise may promote functional recovery following stroke through their beneficial effects. However, no study has determined the effects of NYT and the possible mechanisms of neurorepair and functional recovery after stroke. Aim of the study: This study aimed to investigate the combined effects of NYT and exercise on neuroprotection and functional recovery and the underlying mechanisms in a rat ischemic stroke model. Materials and methods: Stroke was induced with 60-min middle cerebral artery occlusion (MCAO) followed by reperfusion in adult male Sprague-Dawley rats. After stroke, the rats were assigned to four groups: ischemia reperfusion (IR), NYT, exercise (Ex), and NYT + Ex. NYT-treated rats were fed a diet containing 1% NYT one day after stroke. Exercise was performed using a motorized treadmill for 5 days a week (8–15 m/min, 20 min/day), starting 3 days after stroke. The NYT treatment and exercise were continued for 4 weeks after the stroke. Infarct volume, neurological deficits, sensorimotor functions, expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), tropomyosin receptor kinase A (TrkA) and B (TrkB), caspase-3 activity, and the p-Akt/Akt ratio were examined by immunohistochemistry and western blotting. Results: Compared to the IR group, all treated groups indicated reduced infarct volumes. The NYT + Ex group showed significantly improved waking time and beam walking score compared with the IR group. The expression of NGF/TrkA/p-TrkA and BDNF/TrkB was significantly increased in the NYT + Ex group compared with those in the IR group, whereas the number of caspase-3 positive cells around the lesion was significantly lower in the NYT + Ex group than in the IR group. In addition, the ratio of p-Akt/Akt was significantly higher in the NYT + Ex group than in the IR group. Conclusions: This study suggests that NYT in combination with exercise provides neuroprotective effects and improves sensorimotor function by stimulating NGF/TrkA and BDNF/TrkB, and by activating the Akt pathway in ischemic stroke of rats. NYT may be an effective adjunctive agent in post-stroke rehabilitation.

DOI： 10.1016/j.jep.2022.115927

Kikuchi K., Otsuka S., Takada S., Nakanishi K., Setoyama K., Sakakima H., Tanaka E., Maruyama I. . 1,5-anhydro-D-fructose induces anti-aging effects on aging-associated brain diseases by increasing 5’-adenosine monophosphate-activated protein kinase activity via the peroxisome proliferator-activated receptor-γ co-activator-1α/brain-derived neurotrophic factor pathway . Aging15 ( 21 ) 11740 - 11763 2023年

記述言語：日本語出版者・発行元：Aging

5’-Adenosine monophosphate-activated protein kinase (AMPK) is a metabolic sensor that serves as a cellular housekeeper; it also controls energy homeostasis and stress resistance. Thus, correct regulation of this factor can enhance health and survival. AMPK signaling may have a critical role in aging-associated brain diseases. Some in vitro studies have shown that 1,5-anhydro-D-fructose (1,5-AF) induces AMPK activation. In the present study, we experimentally evaluated the effects of 1,5-AF on aging-associated brain diseases in vivo using an animal model of acute ischemic stroke (AIS), stroke-prone spontaneously hypertensive rats (SHRSPs), and the spontaneous senescence-accelerated mouse-prone 8 (SAMP8) model. In the AIS model, intraperitoneal injection of 1,5-AF reduced cerebral infarct volume, neurological deficits, and mortality. In SHRSPs, oral administration of 1,5-AF reduced blood pressure and prolonged survival. In the SAMP8 model, oral administration of 1,5-AF alleviated aging-related decline in motor cognitive function. Although aging reduced the expression levels of peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α) and brain-derived neurotrophic factor (BDNF), we found that 1,5-AF activated AMPK, which led to upregulation of the PGC-1α/BDNF pathway. Our results suggest that 1,5-AF can induce endogenous neurovascular protection, potentially preventing aging-associated brain diseases. Clinical studies are needed to determine whether 1,5-AF can prevent aging-associated brain diseases.

DOI： 10.18632/aging.205228

則松貢輔, 中西和毅, 柿元翔吾, 野島菜央, 谷明, 松崎凌真, 松岡輝樹, 稲留真輝, 加藤夕貴, 立部勇汰, 高田聖也, 大塚章太郎, 角園恵, 榊間春利 . トレッドミル運動と水泳が老化促進マウスの自然発症型変形性膝関節症に及ぼす影響 . 基礎理学療法学advpub ( 0 ) 1 - 10 2023年

記述言語：日本語出版者・発行元：一般社団法人日本基礎理学療法学会

本研究は自然発症型変形性膝関節症（以下，膝OA）を呈する老化促進マウス（SAMP8）を用いて，トレッドミル運動と水泳がOA病態，関節可動域，横径，歩容に及ぼす影響を調べ，運動効果の違いを明らかにすることを目的とした。マウスを非運動群，トレッドミル運動群，水泳群（各6匹）に無作為に分類した。トレッドミル運動は速度10～12 m/min，水泳は水温37～38℃で，15分/ 日，週5日，6週間実施した。非運動群と比較して，両運動群は膝関節可動域や歩幅の改善を示した。さらにII 型コラーゲン陽性軟骨細胞の増加，MMP-13陽性軟骨細胞の減少，滑膜のTNF-α陽性細胞の減少を認めた。特に水泳群の歩幅は有意な改善を認め，軟骨恒常性維持に重要なタンパク質であるII型コラーゲン陽性軟骨細胞は有意に増加していた。本研究は，運動が加齢による軟骨変性を緩和し，関節可動域や歩容を改善させることを示した。また，水泳が膝OA軟骨の恒常性維持や歩容改善に有益であることを示唆した。

DOI： 10.24780/jjptf.jjptf_2023-01

谷明, 宮崎雅司, 中西和毅, 榊間春利 . 遷延性術後痛の軽減やQOL向上に対してWater Walker & Spaを用いた水中運動療法が有効であった一症例 . 理学療法かごしま2 ( 0 ) 56 - 62 2023年

記述言語：日本語出版者・発行元：公益社団法人鹿児島県理学療法士協会

腰部や膝関節の遷延性術後痛によりADL能力とQOLの低下を認める症例に対し，症状緩和を目的にWater Walker & Spa steed proを用いた個別水中運動療法を実施した。症例は60歳代後半の女性で，強い腰殿部痛と膝関節痛のために活動性低下や不眠の症状を呈していた。水中運動療法（40分間/回，週2回，4週間）として，体幹・下肢のストレッチおよび自動運動，バランス練習，水中トレッドミル歩行（水温：33℃，水位：90–110 cm，1.5–2.0 km/h）を実施した。疼痛，身体機能，疾患特異的なADL・QOL，睡眠について評価し，介入前後の比較を実施した。その結果，水中運動療法後，即時的に腰殿部痛や膝痛の軽減を認め，下肢・体幹筋力が増加した。また，膝WOMACや腰部ODI・JOABPEQのスコアが改善し，ADL能力と全体的健康感が向上した。アテネ不眠尺度では不眠の改善と，日中の活動性増加を認めた。個別の水中運動療法によって即時的に疼痛や不眠が軽減し，徐々に活動性が増加しADL能力・QOLが向上した。

DOI： 10.57357/ptkagoshima.2.0_56

松岡輝樹, 松崎凌真, 中西和毅, 榊間春利 . 運動器疾患を有した地域在住高齢者におけるTimed up and go test と日中活動パターンの関係 . 九州理学療法士学術大会誌2023 ( 0 ) 173 2023年

記述言語：日本語出版者・発行元：公益社団法人日本理学療法士協会九州ブロック会

【目的】 Timed up and go test（TUG）は動的バランス能力の評価指標であり、信頼性が高く、下肢筋力、バランス、歩行能力、易転倒性といった日常生活機能との関連性が高い。高齢者の身体機能評価や転倒の予測因子として広く用いられている。Shumway-Cook（Phys Ther. 2000）らは、地域在住高齢者の転倒経験者と非経験者のTUGのcut off値を13.5秒と報告している。これらのcut-off値は調査対象者によって変化し、運動器不安定症では11秒とされている。しかしながら、TUGの値が地域在住高齢者の日中活動パターンに及ぼす影響についてはよく分かっていない。そこで、本研究は、運動器疾患を有する地域在住高齢者を対象にしてTUGと日中活動パターンの関係について検討した。【方法】対象は、運動器疾患を有する地域在住高齢者103名の内、過去1年以内の急性疼痛、認知症、神経疾患、心血管疾患、重度の併存疾患の既往をもつ者、データに欠損がある者30名を除外した73名（男性：16名女性：57名、81.2±13.2歳）とした。調査項目は、過去1年間の転倒経験、TUG、日中活動量の計測を行った。活動量の計測には3次元加速度センサ（OMRON社製、Active style pro HJA-750C）を用い、対象者には1週間装着するよう指示し、8時間/日以上装着したデータを解析に用いた。活動量の解析指標として歩数、生活EX、歩行EXに着目した。EXとは身体活動の強さと量を表す単位で、身体運動強度（METs）と活動時間の積で表される。今回AM6：00～PM23：00における3METs以上6METs未満のEXを分析した。最初に転倒経験者と非経験者において、TUGの値に違いがあるか検討した。次に、今回の対象者全員の平均値が12.9秒であったので、TUGのカットオフ値を13秒に設定し、TUG13秒未満群と13秒以上群の2群に分けて比較検討した。統計学的検定にはGraphPad Prismを用い、群間比較は対応のないt検定あるいはMann-Whitney検定、グループ解析はFisherの正確確率検定、日中活動パターンの比較は二元配置分散分析、TUGと活動量の関係はピアソン相関係数を用いて検討し、有意水準は5%とした。【結果】 TUGの値は、転倒経験者（16.1秒）が非経験者（11.3秒）と比較して有意に高かった（p＜0.01）。1日の歩数において、TUG13秒以上群（1,409歩）は13秒未満群（3,264歩）と比較して有意に減少していた（p＜0.01）。同様に、TUG13秒以上群は13秒未満群と比べて生活EX（11.2と18.6）、歩行EX（1.3と3.0）ともに有意に減少していた（p＜0.05）。1日の時間別平均歩数は12時を境に午前10時と午後2時にピークを迎える2峰性の変化を示し、TUG13秒以上群は午前と午後の歩数が減少していた。歩行EXと生活EXも同様の日内変化を示し、13秒以上群で大きく減少していた。TUG値の増加は歩数や生活EXと有意な負の相関関係を認めた（r=-0.539とr=-0.530、p＜0.05）。【考察】運動器疾患を有する地域在住高齢者は歩行活動と比較して生活活動は比較的維持されていることが報告されている（Sakakima et al., Int J Environ Res Public Health. 2020）。本研究は、TUG値が大きいと転倒リスクが高まると同時に日中の活動パターンに影響を及ぼし、特に日中の歩行活動だけでなく生活活動も減少していることを示唆した。【まとめ】運動器疾患を有する地域在住高齢者において、TUGの値は日中活動パターンと関係しており、在宅での活動性の評価指標として有益である。【倫理的配慮、説明と同意】本研究は鹿児島大学倫理審査委員会の承認を得て実施した（No180050）。ヘルシンキ宣言に基づいて実施した。対象者には十分説明を行い、同意を得た。

DOI： 10.32298/kyushupt.2023.0_173