Updated on 2022/01/12

写真a

 
MIURA Hirohito
 
Organization
Research Field in Dentistry, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Functional Biology and Pharmacology Associate Professor
Title
Associate Professor

Degree

  • 博士(理学) ( 1994.3   東北大学 )

Research Interests

  • 老化

  • 味神経

  • 細胞分化

  • 味覚

  • 細胞分化

  • 味覚

  • taste bud

Research Areas

  • Others / Others  / 感覚生物学

  • Life Science / Food sciences

  • Life Science / Cell biology

  • Life Science / Physiology

  • Life Science / Clinical pharmacy

  • Life Science / Molecular biology

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Education

  • Tohoku University   Graduate School of Science

    1989.4 - 1994.3

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    Country: Japan

  • Tohoku University   Faculty of Science

    1984.4 - 1988.3

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    Country: Japan

Research History

  • コロラド大学 医学部   Senior Research Associate

    2009.5 - 2010.5

  • Kagoshima University   Associate Professor

    2005.4

  • Kagoshima University   Associate Professor

    2005.4

  • 鹿児島大学 大学院医歯学総合研究所   准教授

    2005.4

  • National Food Research Institute   Chief Researcher

    2001.4 - 2005.3

  • 独立行政法人 食品総合研究所   主任研究官

    2000.4 - 2001.3

  • Ministry of Agriculture, Forestry and Fisheries

    1997.4 - 2001.3

  • 三菱化学生命科学研究所   特別研究員

    1994.4 - 1997.3

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Professional Memberships

  • 日本生理学会

    2015.10

  • 味と匂学会

    2015.10

  • 歯科基礎医学会

    2015.10

  • 歯科基礎医学会

  • THE PHYSIOLOGICAL SOCIETY OF JAPAN

  • 日本味と匂学会

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Committee Memberships

  • 味と匂学会   味と匂学会誌編集委員  

    2015.4 - 2021.3   

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    Committee type:Other

  • 歯科基礎医学会   評議員  

    2006.4   

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    Committee type:Other

  • 味と匂学会   評議員  

    1999.5   

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    Committee type:Other

 

Papers

  • Eriko Koyanagi-Matsumura, Hirohito Miura, Mitsuru Saito, Shuitsu Harada .  Type II/III cell composition and NCAM expression in taste buds .  Cell and Tissue Research385 ( 3 ) 557 - 570   2021Type II/III cell composition and NCAM expression in taste budsReviewed International journal

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Taste buds are localized in fungiform (FF), foliate (FL), and circumvallate (CV) papillae on the tongue, and taste buds also occur on the soft palate (SP). Mature elongate cells within taste buds are constantly renewed from stem cells and classified into three cell types, Types I, II, and III. These cell types are generally assumed to reside in respective taste buds in a particular ratio corresponding to taste regions. A variety of cell-type markers were used to analyze taste bud cells. NCAM is the first established marker for Type III cells and is still often used. However, NCAM was examined mainly in the CV, but not sufficiently in other regions. Furthermore, our previous data suggested that NCAM may be transiently expressed in the immature stage of Type II cells. To precisely assess NCAM expression as a Type III cell marker, we first examined Type II and III cell-type markers, IP3R3 and CA4, respectively, and then compared NCAM with them using whole-mount immunohistochemistry. IP3R3 and CA4 were segregated from each other, supporting the reliability of these markers. The ratio between Type II and III cells varied widely among taste buds in the respective regions (Pearson’s r = 0.442 [CV], 0.279 [SP], and − 0.011 [FF]), indicating that Type II and III cells are contained rather independently in respective taste buds. NCAM immunohistochemistry showed that a subset of taste bud cells were NCAM(+)CA4(−). While NCAM(+)CA4(−) cells were IP3R3(−) in the CV, the majority of them were IP3R3(+) in the SP and FF.

    DOI: 10.1007/s00441-021-03452-5

    Scopus

    PubMed

  • Masahiro Heima, Lin Ying-Chu, Hirohito Miura, Hiroko Indo, Yoshihiro Nishitani .  Promising effects of an online studying abroad program with cultural exchange .  Journal of Dental Education   2021Promising effects of an online studying abroad program with cultural exchange

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/jdd.12808

    Scopus

  • Eriko Koyanagi-Matsumura, Hirohito Miura, Mitsuru Saito, Shuitsu Harada .  Taste bud-associated expression of fibroblast growth factors in the circumvallate papillae in adult mice. .  Journal of South Kyushu Dental Society2 ( 1 ) 1 - 11   2020Taste bud-associated expression of fibroblast growth factors in the circumvallate papillae in adult mice.Reviewed

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • Changes of gustatory preference with age in the rat .    35 ( 35 ) 11 - 19   2015.3

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    Language:Japanese  

    The effect of aging on taste were compared between the preference and neural responses from the greater superficial petrosal nerve (GSP) innervating the soft palate and the chorda tympani nerve (CT) innervating the fungiform papillae in the young (5-12 weeks) and aged (19-22 months) Sprague Dawley rat. A two-bottle preference test revealed that younger rats significantly preferred 0.001 M 5'-inosine monophosphate (IMP), 0.01 M mono sodium glutamate (MSG), and binary mixtures of 0.001 M IMP + 0.01 M MSG than deionized water. However, aged rats showed no significant preference to these umami solutions. Among the other four basic taste stimuli, there was no significant difference in preference between young and aged rats. Regardless of the age of the rat, neural responses from the GSP and CT produced robust integrated responses to all three umami solutions the same as used in the two-bottle test. These results indicate that the lack of preference to umami in aged rats is occurred in the central nervous system, suggesting that the loss of preference to umami taste in aged rats may be caused by homeostatic changes in the brain.

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  • Ayumi Nakayama, Hirohito Miura, Makoto Ooki, Shuitsu Harada .  During development intense Sox2 expression marks not only Prox1-expressing taste bud cell but also perigemmal cell lineages .  CELL AND TISSUE RESEARCH359 ( 3 ) 743 - 753   2015.3Reviewed International journal

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    Sox2 is proposed to regulate the differentiation of bipotential progenitor cells into taste bud cells. However, detailed expression of Sox2 remains unclear. In this report, Sox2 expression during taste bud development in the fungiform (FF), circumvallate (CV) and soft palate (SP) areas is examined together with Prox1. First, we immunohistochemically checked Prox1 expression in adults and found that almost all taste bud cells are Prox1-positive. During FF development, intense Sox2 expression was restricted to taste bud primordia expressing Prox1 at E12.5. However, at E14.5, Sox2 was intensely expressed outside the developing taste buds resolving to perigemmal Sox2 expression in adults. In the SP, at E14.5, taste bud primordia emerged as Prox1-expressing cell clusters. However, intense Sox2 expression was not restricted to taste bud primordia but was detected widely in the epithelium. During development, Sox2 expression outside developing taste buds was generally down-regulated but was retained in the perigemmal region similarly to that in the FF. In the CV, the initial stage of taste bud development remained unclear because of the lack of taste bud primordia comparable to that in the FF and SP. Here, we show that Prox1-expressing cells appear in the apical epithelium at E12.5, in the inner trench wall at E17.5 and in the outer trench wall at E18.5. Sox2 was again not restricted to developing taste bud cells expressing Prox1 during CV development. The expression patterns support that Sox2 does not serve as a cell fate selector between taste bud cells and surrounding keratinocytes but rather may contribute to them both.

    DOI: 10.1007/s00441-014-2076-5

    Web of Science

  • Hirohito Miura, Jennifer K. Scott, Shuitsu Harada, Linda A. Barlow .  Sonic hedgehog-Expressing Basal Cells Are General Post-Mitotic Precursors of Functional Taste Receptor Cells .  DEVELOPMENTAL DYNAMICS243 ( 10 ) 1286 - 1297   2014.10Reviewed

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    Background: Taste buds contain similar to 60 elongate cells and several basal cells. Elongate cells comprise three functional taste cell types: I, glial cells; II, bitter/sweet/umami receptor cells; and III, sour detectors. Although taste cells are continuously renewed, lineage relationships among cell types are ill-defined. Basal cells have been proposed as taste bud stem cells, a subset of which express Sonic hedgehog (Shh). However, Shh1 basal cells turn over rapidly suggesting that Shh1 cells are post-mitotic precursors of some or all taste cell types. Results: To fate map Shh-expressing cells, mice carrying ShhCreER(T2) and a high (CAG-CAT-EGFP) or low (R26RLacZ) efficiency reporter allele were given tamoxifen to activate Cre in Shh1 cells. Using R26RLacZ, lineage-labeled cells occur singly within buds, supporting a post-mitotic state for Shh1 cells. Using either reporter, we show that Shh1 cells differentiate into all three taste cell types, in proportions reflecting cell type ratios in taste buds (I > II > III). Conclusions: Shh1 cells are not stem cells, but are post-mitotic, immediate precursors of taste cells. Shh1 cells differentiate into each of the three taste cell types, and the choice of a specific taste cell fate is regulated to maintain the proper ratio within buds. (C) 2014 Wiley Periodicals, Inc.

    DOI: 10.1002/DVDY.24121

    Web of Science

  • Hirohito Miura, Yuko Kusakabe, Kento Hashido, Akihiro Hino, Makoto Ooki, Shuitsu Harada .  The glossopharyngeal nerve controls epithelial expression of Sprr2a and Krt13 around taste buds in the circumvallate papilla .  NEUROSCIENCE LETTERS580   147 - 152   2014.9

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    Publisher:ELSEVIER IRELAND LTD  

    Tastants reach the tip of taste bud cells through taste pores which are openings in the epithelium. We found Sprr2a is selectively expressed in the upper layer of the epithelium surrounding taste buds in the circumvallate papilla (CV) where the epithelium is organized into taste pores. Sprr2a is a member of a small proline-rich protein family, which is suggested to be involved in the restitution/migration phase of epithelial wound healing. The expression of Sprr2a was restricted to the upper layer and largely segregated with Ptch1 expression that is restricted to the basal side of the epithelium around the taste buds. Denervation resulted in the gradual loss of Sprr2a-expressing cells over 10 days similarly to that of taste bud cells which is in contrast to the rapid loss of Ptch1 expression. We also found that denervation caused an increase of Keratin (Krt)13 expression around taste buds that corresponded with the disappearance of Sprr2a and Ptch1 expression. Taste buds were surrounded by Krt13-negative cells in the CV in control mice. However, at 6 days post-denervation, taste buds were tightly surrounded by Krt13-positive cells. During taste bud development, taste bud cells emerged together with Krt13-negtive cells, and Sprr2a expression was increased along with the progress of taste bud development. These results demonstrate that regional gene expression surrounding taste buds is associated with taste bud formation and controlled by the innervating taste nerve. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2014.08.012

    Web of Science

  • Hirohito Miura, Makoto Ooki, Norikazu Kanemaru, Shuitsu Harada .  Decline of umami preference in aged rats .  NEUROSCIENCE LETTERS577   56 - 60   2014.8Reviewed International journal

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER IRELAND LTD  

    The effects of aging on the umami sensation were compared between the preference and neural responses from the greater superficial petrosal nerve (GSP innervating the soft palate) and the chorda tympani nerve (CT innervating the fungiform papillae) in the Sprague Dawley rat. A two-bottle preference test revealed that younger rats (5-12 weeks) preferred significantly 0.001 M 5'-inosine monophosphate (IMP), 0.01 M mono sodium glutamate (MSG), and binary mixtures of 0.001 M IMP + 0.01 M MSG than deionized water. However, aged rats (21-22 months) showed no significant preference to these umami solutions compared to deionized water. Among the other four basic taste stimuli, there were no significant differences in preference between young and aged rats. Regardless of the age of the rat, neural responses from the GSP and CT produced robust integrated responses to all three umami solutions used in the two-bottle tests. These results indicate that the lack of preference to umami in aged rats is a central nervous system phenomenon and suggests that the loss of preference to umami taste in aged rats is caused by homeostatic changes in the brain incurred by aging. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.neulet.2014.06.018

    Web of Science

  • Hiroshi Tomonari, Hirohito Miura, Makoto Ooki, Ayumi Nakayama, Shuitsu Harada .  Diverse contributions of Tas1r2/Tas2rs within the rat and mouse soft palate to sweet and bitter neural responses. .  Neuroscience letters569   63 - 7   2014.5Reviewed International journal

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Neural responses to sweet and bitter stimuli in the rat and mouse are compared to the expression of the molecular taste receptors, Tas1r2/Tas2rs. Integrated taste responses from the greater superficial petrosal nerve (GSP) innervating the soft palate (SP) and the chorda tympani (CT) nerve innervating the fungiform papillae (FF) were recorded in C57BL mice and SD rats. The sum of the phasic and tonic response magnitudes (SRM) was calculated by summating all relative mean responses to a concentration series of QHCl (10(-6)-10(-2)M) or Suc (10(-4)-1.0M). Molecular expression was analyzed by double-colored in situ hybridization for Gα-gustducin with Tas1r2 or Tas2rs in the SP and FF. The vast majority of cells expressing Tas1r2 or Tas2rs were included in Gα-gustducin-expressing cells in the SP of both species. Unexpectedly, a comparison between species revealed that the SRM from the GSP is not positively correlated with receptor expression in the SP. In the rat SP, the percentage of Tas2rs with Gα-gustducin (Tas2rs/gust, 65%) was twice larger than that for Tas1r2/gust (33%), while the SRM to Suc in the rat GSP was 1.5 times (tonic and phasic) larger than that to QHCl. In the mouse SP, the percentage of Tas2rs/gust (46%) was less than that in the rat and similar to that of Tas1r2/gust (40%). However, the SRM to QHCl in the mouse GSP was 2.4 (phasic) and 4.7 (tonic) times larger than to Suc. On the other hand, threshold to Suc in the rat GSP was 10(-3)M, one log unit lower than in mouse, and the threshold to QHCl in the mouse GSP was 10(-6)M, one log unit lower than in rat. These results suggest that the robust GSP response to Suc in rat and to QHCl in mouse likely do not depend upon a large number of taste cells expressing the taste receptors Tas1r2 for Suc or Tas2rs for QHCl, but upon a higher density of Tas1r2/Tas2rs within the respective taste cells of the two species.

    DOI: 10.1016/j.neulet.2014.03.014

    PubMed

  • Hiroshi Tomonari, Hirohito Miura, Ayumi Nakayama, Eriko Matsumura, Makoto Ooki, Yuzo Ninomiya, Shuitsu Harada .  Gα-gustducin is extensively coexpressed with sweet and bitter taste receptors in both the soft palate and fungiform papillae but has a different functional significance. .  Chemical senses37 ( 3 ) 241 - 51   2012.3

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    To clarify the regional differences in the expression and functional significance of Gα-gustducin in soft palate (SP) and fungiform (FF) taste buds, we examined the coexpression of Gα-gustducin with taste receptors and the impact of Gα-gustducin knockout (gKO) on neural responses to several sweet and bitter compounds. Sweet responses from both the greater superficial petrosal (GSP) and chorda tympani (CT) nerves in gKO mice were markedly depleted, reflecting overlapping expression of Gα-gustducin and Tas1r2. However, although Gα-gustducin was expressed in 87% and 88% of Tas2rs cells in the SP and FF, respectively, there were no statistically significant differences in the CT responses to quinine-HCl (QHCl) and denatonium (Den) between gKO and wild-type (WT) mice. In contrast, GSP responses to these compounds were markedly reduced in gKO mice with an apparent elevation of thresholds (>10-fold). These results suggest that 1) Gα-gustducin plays a critical role in sweet transduction in both the SP and the FF, 2) other Gα subunits coexpressed with Gα-gustducin in the FF are sufficient for responses to QHCl and Den, and 3) robust GSP responses to QHCl and Den occur in the SP by a Gα-gustducin-dependent mechanism, which is absent in the FF.

    DOI: 10.1093/chemse/bjr098

    PubMed

  • 中山歩, 三浦裕仁, 大木誠, 日下部裕子, 原田秀逸 .  味蕾の発生過程における味蕾基底細胞マーカーとSox2の発現 .  日本味と匂学会誌18 ( 3 ) 223 - 224   2011.12味蕾の発生過程における味蕾基底細胞マーカーとSox2の発現Reviewed

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    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

  • 日下部裕子, 進藤由美子, 河合崇行, 川本恵子, 三浦裕仁 .  レセプター型チロシンキナーゼc-kit のマウス味蕾における発現様式 .  日本味と匂学会誌18 ( 3 ) 211 - 212   2011.12レセプター型チロシンキナーゼc-kit のマウス味蕾における発現様式Reviewed

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  • Yoichiro Shindo, Kana Morishita, Eiichi Kotake, Hirohito Miura, Piero Carninci, Jun Kawai, Yoshihide Hayashizaki, Akihiro Hino, Tomomasa Kanda, Yuko Kusakabe .  FXYD6, a Na,K-ATPase Regulator, Is Expressed in Type II Taste Cells .  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY75 ( 6 ) 1061 - 1066   2011.6Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    Taste buds contain three types of taste cells. Each type can respond to taste stimulation, and type II and III taste cells are electrically excitable. However, there are differences between the properties of type II and ill taste cells. In this study, we found that Fxyd6, an Na,K-ATPase regulator gene, is expressed in type II taste cells in the taste buds of mice. Double-labeled in situ hybridization analysis showed that Fxyd6 was coexpressed with transient receptor potential cation channel, subfamily M, member 5 (Trpm5), a critical component of the sweet, bitter, and umami taste signal transduction pathways and that it was specifically expressed in type II taste cells. We also found that taste cells frequently coexpressed Fxyd6 and Na,K-ATPase beta 1. These results indicate the presence of an inherent mechanism that regulated transmembrane Na+ dynamics in type II taste cells.

    DOI: 10.1271/bbb.100718

    Web of Science

  • OOKI Makoto, MIURA Hirohito, TOMONARI Hiroshi, NAKAYAMA Ayumi, MARGOLSKEE Robert F., NINOMIYA Yuzo, HARADA Shuitsu .  Licking of gustducin knockout mice to bitter tastants .  The Japanese journal of taste and smell research17 ( 3 ) 223 - 224   2010.12Reviewed

  • H. Miura, L. A. Barlow .  Taste bud regeneration and the search for taste progenitor cells .  ARCHIVES ITALIENNES DE BIOLOGIE148 ( 2 ) 107 - 118   2010.6

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    Publisher:UNIV PISA  

    While the taste periphery has been studied for over a century, we are only beginning to understand how this important sensory system. is maintained throughout adult life. With the advent of molecular genetics in rodent models, and the upswing in translational approaches that impact human patients, we expect the field will make significant advances in the near future.

    Web of Science

  • Yoichiro Shindo, Mi-Ryung Kim, Hirohito Miura, Toshifumi Yuuki, Tomomasa Kanda, Akihiro Hino, Yuko Kusakabe .  Lrmp/Jaw1 is Expressed in Sweet, Bitter, and Umami Receptor-Expressing Cells .  CHEMICAL SENSES35 ( 2 ) 171 - 177   2010.2

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    Publisher:OXFORD UNIV PRESS  

    Inositol 1,4,5-triphosphate-mediated calcium (IP(3)-Ca(2+)) signal cascade is an essential process in sweet, bitter, and umami taste signal transduction. Although the main components of this cascade have been identified, the candidate regulators of them in taste tissues are still unclear. In an effort to identify genes involved in taste signal transduction, we found that a gene encoding lymphoid-restricted membrane protein (Lrmp/Jaw1) was expressed in mouse taste tissues. Here we report that Lrmp/Jaw1 is specifically expressed in sweet, bitter, and umami taste receptor-expressing cells of mouse circumvallate, foliate, and fungiform papillae. In addition to this specific expression patterns, we found that Lrmp/Jaw1 is associated with type III IP(3) receptor (IP(3)R3) via its coiled-coil domain in the COS7 heterologous expression system. These results raise the possibility that Lrmp/Jaw1 interacts with IP(3)R3 in taste cells and suggest an important role for Lrmp/Jaw1 in the IP(3)-Ca(2+) signal cascade in sweet, bitter, and umami taste signal transduction.

    DOI: 10.1093/chemse/bjp097

    Web of Science

  • 日下部裕子, 進藤洋一郎, 三浦裕仁, カルニンチ ピエロ, 河合純, 林崎良英, 二ノ宮裕三, 日野明寛 .  味蕾細胞特異的に発現する遺伝子の口腔内の部位による多様な発現様式 .  味と匂学会誌16 ( 3 ) 269 - 270   2009.12味蕾細胞特異的に発現する遺伝子の口腔内の部位による多様な発現様式Reviewed

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  • 友成博, 三浦裕仁, 中山歩, 松村江梨子, 進藤洋一郎, 日下部裕子, Robert F Margolskee, 二ノ宮裕三, 原田秀逸 .  軟口蓋味蕾の味覚受容体発現パターンとgustducin KOマウスの軟口蓋味蕾の味覚応答解析 .  日本味と匂学会誌16 ( 3 ) 319 - 320   2009.12軟口蓋味蕾の味覚受容体発現パターンとgustducin KOマウスの軟口蓋味蕾の味覚応答解析Reviewed

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  • KUSAKABE Yuko, SHINDO Yoichiro, MIURA Hirohito, CARNINCI Piero, KAWAI Jun, HAYASHIZAKI Yoshihide, NINOMIYA Yuzo, HINO Akihiro .  Regional expression patterns of taste cell-specific genes in the mouse oral cavity .  The Japanese journal of taste and smell research16 ( 3 ) 269 - 270   2009.12Reviewed

  • MATSUMURA Eriko, MIURA Hirohito, NAKAYAMA Ayumi, TOMONARI Hiroshi, OOKI Makoto, HARADA Shuitsu .  Fibroblast growth factor (Fgf) and its receptor (Fgfr) expression in mouse taste buds analyzed by RT-PCR .  The Japanese journal of taste and smell research16 ( 3 ) 291 - 292   2009.12Reviewed

  • 友成博, 三浦裕仁, 中山歩, 松村江梨子, 進藤洋一郎, 日下部裕子, Robert F Margolskee, 二ノ宮裕三, 原田秀逸 .  軟口蓋味蕾の味覚受容体発現パターンとgustducinノックアウトマウスの軟口蓋味蕾の味覚応答解析 .  日本味と匂学会誌15 ( 3 ) 419 - 422   2008.12軟口蓋味蕾の味覚受容体発現パターンとgustducinノックアウトマウスの軟口蓋味蕾の味覚応答解析Reviewed

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  • 中山歩, 友成博, 三浦裕仁, 松村江梨子, 原田秀逸 .  大錐体神経と鼓索神経とをつなぎ換えて再生させた味蕾の細胞分化 .  日本味と匂学会誌15 ( 3 ) 357 - 358   2008.12大錐体神経と鼓索神経とをつなぎ換えて再生させた味蕾の細胞分化Reviewed

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Yoichiro Shindo, Hirohito Miura, Piero Carninci, Jun Kawai, Yoshihide Hayashizaki, Yuzo Ninomiya, Akihiro Hino, Tomomasa Kanda, Yuko Kusakabe .  G alpha 14 is a candidate mediator of sweet/umami signal transduction in the posterior region of the mouse tongue .  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS376 ( 3 ) 504 - 508   2008.11

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    Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Gustducin, a Got Subunit expressed in taste cells, is known as a key molecule for sweet, Umami and bitter taste signal transduction. However, previous studies demonstrated that the contribution of gustducin to the sweet/umami responses in the posterior region of the tongue is less than that ill the anterior region, implying the existence of another G alpha subunit mediating sweet/umami taste signal transductional. Here, we Propose G alpha 14, a member of G alpha q family, as the candidate mediator. G alpha 14 was found in our Subtracted full-length cDNA library derived from mouse circumvallate papillae (CV) and expressed in a subset of taste cells in CV and foliate papillae, but not in fungiform papillae and soft palate. G alpha 14 was co-expressed with T1r3, a sweet/umami taste receptor, but not with gustducin in CV. These results suggest the important roles of G alpha 14 in sweet/umami taste signal transduction in the posterior region of the tongue. (c) 2008 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2008.09.035

    Web of Science

  • Ayumi Nakayama, Hirohito Miura, Yoichiro Shindo, Yuko Kusakabe, Hiroshi Tomonari, Shuitsu Harada .  Expression of the basal cell markers of taste buds in the anterior tongue and soft palate of the mouse embryo. .  The Journal of comparative neurology509 ( 2 ) 211 - 24   2008.7Reviewed International journal

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Although embryonic expression of Shh in the fungiform papilla placodes has a critical role in fungiform papilla patterning, it remains unclear whether its appearance indicates the differentiation of the basal cells of taste buds. To examine the embryonic development of the basal cells, the expression of Shh, Prox1, and Mash1 was determined in the anterior tongue and soft palate in mouse embryos by in situ hybridization. In the anterior tongue, Prox1 was coexpressed with Shh from the beginning of Shh expression in the fungiform papilla placodes at E12.5. Shh was expressed in the soft palate in a band-like pattern in the anteriormost region and in a punctate pattern in the posterior region at E14.5. The number (21.4 +/- 4.3, at E14.5) of locations where Shh was observed (i.e., spots) rapidly increased and reached a peak level (54.8 +/- 4.0 at E15.5). Also in the soft palate, Prox1 was coexpressed with Shh from the beginning of Shh expression. These results suggest that basal cell differentiation occurs synchronously with the patterning of Shh spots both in the anterior tongue and in the soft palate. In contrast, Mash1 expression lagged behind the expression of Shh and Prox1 and began after the number of Shh spots had reached its peak level in the soft palate. Furthermore, immunohistochemistry of PGP9.5 and Shh revealed that epithelial innervation slightly preceded Mash1 expression both in the tongue and in the soft palate. This is the first report describing the time courses of the embryonic expression of basal cell markers of taste buds.

    DOI: 10.1002/cne.21738

    PubMed

  • 友成博,三浦裕仁,中山歩,Robert F Margolskee,二ノ宮裕三,原田秀逸 .  gustducinノックアウトマウスの軟口蓋味蕾の味覚応答解析 .  日本味と匂学会誌14 ( 3 ) 403 - 406   2007.12gustducinノックアウトマウスの軟口蓋味蕾の味覚応答解析Reviewed

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  • 日下部裕子、森下加奈、進藤洋一郎、三浦裕仁、Carninci Piero、河合純、林崎良英、日野明寛 .  Na-KATPase レギュレーター Fxyd6の味蕾における発現 .  日本味と匂学会誌14 ( 3 ) 269 - 270   2007.12Na-KATPase レギュレーター Fxyd6の味蕾における発現Reviewed

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  • NAKAYAMA Ayumi, MIURA Hirohito, KATO Hiromi, SHINDO Yoichiro, KUSAKABE Yuko, HINO Akihiro, TOMONARI Hiroshi, HARADA Shuitsu .  Differentiation and innervation of the basal cells of taste buds during mouse embryogenesis .  The Japanese journal of taste and smell research14 ( 3 ) 399 - 402   2007.12Reviewed

  • Hirohito Miura, Ayumi Nakayama, Yoichiro Shindo, Yuko Kusakabe, Hiroshi Tomonari, Shuitsu Harada .  Expression of gustducin overlaps with that of type III IP3 receptor in taste buds of the rat soft palate. .  Chemical senses32 ( 7 ) 689 - 96   2007.9Reviewed International journal

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    Type III IP3 receptor (IP3R3) is one of the common critical calcium-signaling molecules for sweet, umami, and bitter signal transduction in taste cells, and the total IP3R3-expressing cell population represents all cells mediating these taste modalities in the taste buds. Although gustducin, a taste cell-specific G-protein, is also involved in sweet, umami, and bitter signal transduction, the expression of gustducin is restricted to different subsets of IP3R3-expressing cells by location in the tongue. Based on the expression patterns of gustducin and taste receptors in the tongue, the function of gustducin has been implicated primarily in bitter taste in the circumvallate (CV) papillae and in sweet taste in the fungiform (FF) papillae. However, in the soft palate (SP), the expression pattern of gustducin remains unclear and little is known about its function. In the present paper, the expression patterns of gustducin and IP3R3 in taste buds of the SP and tongue papillae in the rat were examined by double-color whole-mount immunohistochemistry. Gustducin was expressed in almost all (96.7%) IP3R3-expressing cells in taste buds of the SP, whereas gustducin-positive cells were 42.4% and 60.1% of IP3R3-expressing cells in FF and CV, respectively. Our data suggest that gustducin is involved in signal transduction of all the tastes of sweet, umami, and bitter in the SP, in contrast to its limited function in the tongue.

    DOI: 10.1093/chemse/bjm036

    PubMed

  • HARADA Shuitsu, OOKI Makoto, TOMONARI Hiroshi, NAKAYAMA Ayumi, MIURA Hirohito .  Gustatory responses from the soft palate in mice .  The Japanese journal of taste and smell research13 ( 3 ) 545 - 546   2006.12

  • Hirohito Miura, Yuko Kusakabe, Shuitsu Harada .  Cell lineage and differentiation in taste buds .  ARCHIVES OF HISTOLOGY AND CYTOLOGY69 ( 4 ) 209 - 225   2006.12Invited Reviewed International journal

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    Mammalian taste buds are maintained through continuous cell renewal so that taste bud cells are constantly generated from progenitor cells throughout life. Taste bud cells are composed of basal cells and elongated cells. Elongated cells are derived from basal cells and contain taste receptor cells (TRC). Morphologically, elongated cells consist of three distinct types of cells: Types I, II and III. In contrast to the remarkable progress in understanding of the molecular basis for taste reception, the mechanisms of taste bud maintenance have remained a major area of inquiry. In this article, we review the expression of regulatory genes in taste buds and their involvement in taste bud cell differentiation. Three major topics include: 1) the Sonic hedgehog (Shh)-expressing cell in the basal cell in taste buds as a transient precursor of elongated cells and as a signal center for the proliferation of progenitor cells; 2) the Mash1-expressing cell as an immature cell state of both Type II and Type III cells and as a mature cell state of Type III cell; and 3) the nerve dependency of gene expression in taste buds. Problems in the application of NCAM for the type III cell marker are also discussed.

    DOI: 10.1679/aohc.69.209

    Web of Science

  • 三浦 裕仁 .  味覚の受容と味蕾細胞分化の分子メカニズム .  鹿児島大学歯学部紀要26   27 - 37   2006.3

  • 原田秀逸,大木誠,中山歩,三浦裕仁 .  マウス軟口蓋味蕾の味覚応答 .  日本味と匂学会誌12 ( 3 ) 317 - 318   2005.12マウス軟口蓋味蕾の味覚応答Reviewed

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  • 中山歩,三浦裕仁,加藤ひろみ,日下部裕子,日野明寛,原田秀逸 .  マウス発生過程における味蕾基底細胞マーカー遺伝子の発現解析 .  日本味と匂学会誌12 ( 3 ) 343 - 344   2005.12マウス発生過程における味蕾基底細胞マーカー遺伝子の発現解析Reviewed

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  • KUSAKABE Yuko, SHINDO Yoichiro, MIURA Hirohito, KIM Mi-Ryung, SHIMIZU Madoka, NINOMIYA Yuzo, HINO Akihiro .  Identification of novel taste bud-specific genes and their functions in gustatory sensation .  The Japanese journal of taste and smell research12 ( 3 ) 255 - 256   2005.12

  • MIURA Hirohito, KATO Hiromi, KUSAKABE Yuko, NAKAYAMA Ayumi, KARIATSUMARI Maiko, NINOMIYA Yuzo, HINO Akihiro, HARADA Shuitsu .  The expression of type III cell marker NCAM and possible cell lineage relationships in mouse taste buds .  The Japanese journal of taste and smell research12 ( 3 ) 345 - 346   2005.12

  • H Miura, H Kato, Y Kusakabe, Y Ninomiya, A Hino .  Temporal changes in NCAM immunoreactivity during taste cell differentiation and cell lineage relationships in taste buds .  CHEMICAL SENSES30 ( 4 ) 367 - 375   2005.5

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    Publisher:OXFORD UNIV PRESS  

    Neural cell adhesion molecule (NCAM) is a type III cell marker in the taste buds. In order to clarify the cell type of Mash1-expressing cells in taste buds, expression of NCAM was examined in Mash1-expressing taste cells of adult mice in comparison with gustducin- and T1r3-expressing cells, using a combination of NCAM immunohistochemistry and in situ hybridization. About 98% of Mash1-expressing cells were NCAM immunopositive (IP), suggesting that Mash1-expressing cells should be categorized as type III cells. Unexpectedly, small subsets of gustducin- and T1r3-expressing cells were also found to be NCAM-IP, contradicting previous immunohistochemical studies in rats, in which gustducin-IP cells were observed specifically in type II cells, which do not have NCAM immunoreactivity. Examinations of developing taste buds showed temporal changes in the ratio of NCAM-IP cells in gustducin- and T1r3-expressing cells; the ratio of NCAM-IP cells in these gene-expressing cells were similar to 90% at 0.5 days after birth and decreased markedly during development. In contrast, the majority of Mash1-expressing cells showed constant NCAM immunoreactivity throughout development. In addition, BrdU-labeling experiments showed that the differentiation of Mash1-expressing cells precedes those of gustducin- and T1r3-expressing cells in taste buds of adult mice. These results suggest that T1r3- and gustducin-expressing cells are NCAM-IP at the beginning of cell differentiation, and that NCAM immunoreactivity in gustducin- and T1r3-expressing cells might remain from the previous developmental stage expressing Mash1.

    DOI: 10.1093/chemse/bji031

    Web of Science

  • Y Kusakabe, MR Kim, H Miura, Y Shindo, Y Ninomiya, A Hino .  Regional expression patterns of T1r family in the mouse tongue .  CHEMICAL SENSES30   I23 - i24   2005.1

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    Publisher:OXFORD UNIV PRESS  

    DOI: 10.1093/chemse/bjh094

    Web of Science

  • H Miura, H Kato, Y Kusakabe, M Tagami, J Miura-Ohnuma, T Ookura, Y Shindo, Y Ninomiya, A Hino .  Shh signaling and regulatory gene expression in mouse taste buds .  CHEMICAL SENSES30   I50 - i51   2005.1

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    Publisher:OXFORD UNIV PRESS  

    DOI: 10.1093/chemse/bjh108

    Web of Science

  • H Miura, H Kato, Y Kusakabe, M Tagami, J Miura-Ohnuma, Y Ninomiya, A Hino .  A strong nerve dependence of Sonic hedgehog expression in basal cells in mouse taste bud and an autonomous transcriptional control of genes in differentiated taste cells .  CHEMICAL SENSES29 ( 9 ) 823 - 831   2004.11Reviewed International journal

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    The nerve-dependency of gene expression in mouse taste bud was examined through an analysis of changes in gene expression in and around the taste buds in circumvallate papillae after surgery of cranial nerve IXth (glossopharyngeal nerve). The number of cells expressing T1r3, gustducin, Mash1 and Nkx2.2 gradually decreased after denervation. However, the expression intensity of these genes was barely influenced by denervation, and strong expression was observed at 6 days after denervation. In contrast, the basal cell-specific Sonic hedgehog (Shh) expression in the taste buds was decreased markedly at 6 h after denervation. In the regeneration process of taste buds, Shh expression was observed during a very early phase before taste bud formation. These results indicate the autonomous transcriptional control of genes in differentiated taste cells and the strong nerve-dependency of Shh expression in basal cells. Furthermore, in order to reveal the mitotic activity of Shh-expressing cells in taste buds, the BrdU-labeling experiments were performed using a combination of BrdU-immunohistochemistry and in situ hybridization. BrdU-signal was very rarely observed in Shh-expressing cells immediately after BrdU injection, and the signals were noted mainly in Ptc-expressing cells. BrdU signals rapidly increased in Shh-expressing cells in following 12 h and began to decrease after 2 days post-injection. These results suggest that most Shh-expressing cells are not mitotically active, but that Shh-expressing cells may be in the early transient developmental state of taste cells in taste buds.

    DOI: 10.1093/chemse/bjh248

    Web of Science

  • N Shigemura, R Ohta, Y Kusakabe, H Miura, A Hino, K Koyano, K Nakashima, Y Ninomiya .  Leptin modulates behavioral responses to sweet substances by influencing peripheral taste structures .  ENDOCRINOLOGY145 ( 2 ) 839 - 847   2004.2

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    Publisher:ENDOCRINE SOC  

    Leptin is a hormone that regulates body weight homeostasis mainly via the hypothalamic functional leptin receptor Ob-Rb. Recently, we proposed that the taste organ is a new peripheral target for leptin. Leptin selectively inhibits mouse taste cell responses to sweet substances and thereby may act as a sweet taste modulator. The present study further investigated leptin action on the taste system by examining expression of Ob-Rb in taste cells and behavioral responses to sweet substances in leptin-deficient ob/ob, and Ob-Rb-deficient db/db mice and their normal litter mates. RT-PCR analysis showed that Ob-Rb was expressed in taste cells in all strains tested. The db/db mice, however, had a RT-PCR product containing an abnormal db insertion that leads to an impaired shorter intracellular domain. In situ hybridization analysis showed that the hybridization signals for normal Ob-Rb mRNA were detected in taste cells in lean and ob/ob mice but not in db/db mice. Two different behavioral tests, one using sweet-bitter mixtures as taste stimuli and the other a conditioned taste aversion paradigm, demonstrated that responses to sucrose and saccharin were significantly decreased after ip injection of leptin in ob/ob and normal littermates, but not in db/db mice. These results suggest that leptin suppresses behavioral responses to sweet substances through its action on Ob-Rb in taste cells. Such taste modulation by leptin may be involved in regulation for food intake.

    DOI: 10.1210/en.2003-0602

    Web of Science

  • 三浦裕仁,加藤ひろみ,日下部裕子,田上みづほ,三浦??大沼純,二ノ宮裕三,日野明寛 .  マウス味蕾におけるMash1とNkx2.2 およびProx1と .  日本味と匂学会誌10 ( 3 ) 657 - 658   2003.12マウス味蕾におけるMash1とNkx2.2 およびProx1とReviewed

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  • MR Kim, Y Kusakabe, H Miura, Y Shindo, Y Ninomiya, A Hino .  Regional expression patterns of taste receptors and gustducin in the mouse tongue .  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS312 ( 2 ) 500 - 506   2003.12

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    Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    In order to understand differences in taste sensitivities of taste bud cells between the anterior and posterior part of tongue, it is important to analyze the regional expression patterns of genes related to taste signal transduction on the tongue. Here we examined the expression pattern of a taste receptor family, the T1r family, and gustducin in circumvallate and fungiform papillae of the mouse tongue using double-labeled in situ hybridization. Each member of the T1r family was expressed in both circumvallate and fungiform papillae with some differences in their expression patterns. The most striking difference between fungiform and circumvallate papillae was observed in their co-expression patterns of T1r2, T1r3, and gustducin. T1r2-positive cells in fungiform papillae coexpressed T1r3 and gustducin, whereas T1r2 and T1r3 double-positive cells in circumivallate papillae merely expressed gustducin. These results suggested that in fungiform papillae, gustducin might play a role in the sweet taste signal transduction cascade mediated by a sweet receptor based on the T1r2 and T1r3 combination, in fungiform papillae. (C) 2003 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2003.10.137

    Web of Science

  • 日下部 裕子, 金 美令, 三浦 裕仁, 進藤 洋一郎, 岡野 正代, 二ノ宮 裕三, 日野 明寛 .  味受容体ファミリーT1Rsとgustducinの有郭乳頭及び茸状乳頭における発現様式の比較 .  日本味と匂学会誌10 ( 3 ) 415 - 416   2003.12

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  • H Miura, Y Kusakabe, H Kato, J Miura-Ohnuma, M Tagami, Y Ninomiya, A Hino .  Co-expression pattern of Shh with Prox1 and that of Nkx2.2 with Mash1 in mouse taste bud .  GENE EXPRESSION PATTERNS3 ( 4 ) 427 - 430   2003.8Reviewed International journal

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    In mammals, taste buds are maintained by continuous turnover of cells, even in adulthood. Cell proliferation and differentiation continue to produce taste cells, which express various genes related to taste reception. We found the co-expression of Sonic hedgehog (Shh) with Prox1 and that of Nkx2.2 with Mash1 in adult mouse taste buds. Whereas Prox1 was expressed strongly in cells in the basal region of mouse taste buds where Shh was co-expressed, it was expressed weakly in almost all taste bud cells lacking Shh expression. At 0.5 day after birth, when taste cells have not yet differentiated, the expressions of Shh and Prox1 completely overlapped in the epithelium of circumvallate papillae. Nkx2.2 was observed in cells expressing Mash1, but not in cells expressing genes related to taste reception, such as gustducin and TIR3. Almost all fusiform cells expressing Mash1 co-expressed Nkx2.2, while the majority of round cells expressing Mash l in the basal region of taste buds lacked Nkx2.2 expression. (C) 2003 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S1567-133X(03)00081-4

    Web of Science

  • N Shigemura, H Miura, Y Kusakabe, A Hino, Y Ninomiya .  Expression of leptin receptor (Ob-R) isoforms and signal transducers and activators of transcription (STATs) mRNAs in the mouse taste buds .  ARCHIVES OF HISTOLOGY AND CYTOLOGY66 ( 3 ) 253 - 260   2003.8Reviewed International journal

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    Leptin is a hormone that regulates food intake, energy expenditure and body weight. Our previous studies have demonstrated that the taste organ is a new peripheral target for leptin in mice. Leptin selectively inhibits the responses of taste nerves and receptor cells to sweet substances without affecting responses to sour, salty, and bitter substances. Still, there is no convincing evidence for the existence of leptin receptors (Ob-Rs) in taste receptor cells, especially the functional isoform Ob-Rb. We investigated the expression of 5 different Ob-R isoforms (a-e) and 6 STAT (signal transducers and activators of transcription) members in mouse taste cells. STATs are considered to be involved in the leptin signaling via Ob-Rb. Semiquantitative RT-PCR analysis showed that Ob-Rb was expressed in the taste buds of the fungiform and circumvallate papillae, but not so clearly in the surrounding epithelial tissue. The expression pattern among the three different tissues was similar to that of the taste cell specific G-protein, a-gustducin. The other Ob-R isoforms were widely detected in either the taste papillae or the epithelial tissue. Among 6 STAT members, STAT3 showed the highest relative abundance of mRNA in the taste buds. Consistently, in situ hybridization analysis showed that while Ob-Rb and STAT3 signals were detected in some taste bud cells, the signals were not clearly observed in the epithelial tissue cells. In conclusion, the present study provides evidence of the existence of the leptin receptor, Ob-Rb, and STAT3 in the mouse taste bud cells. This finding further confirms the involvement of leptin in the control of taste sensitivities to sweet substances in mice.

    DOI: 10.1679/aohc.66.253

    Web of Science

  • MIURA Hirohito, KUSAKABE Yuko, NINOMIYA Yuzo, MOTOYAMA Jun, KATO Hiromi, TAGAMI Mizuho, HINO Akihiro .  Shh and Ptc are associated with taste bud maintenance in the adult mouse .  The Japanese journal of taste and smell research9 ( 3 ) 605 - 606   2002.12Shh and Ptc are associated with taste bud maintenance in the adult mouse

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  • OH Seong-Hee, MIURA Hirohito, KUSAKABE Yuko, KATO Hiromi, SUZUKI Rika, YOKOYAMA Minesuke, HINO Akihiro .  Analyses to saccharin responses in gustducin-GFP mouse taste sells .  The Japanese journal of taste and smell research9 ( 3 ) 511 - 512   2002.12Analyses to saccharin responses in gustducin-GFP mouse taste sells

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  • SINDO Yoichiro, KUSAKABE Yuko, KIM Mi-Ryung, HASHIMOTO Rika, TAGAMI Mizuho, KATO Hiromi, MIURA Hirohito, HINO Akihiro .  Screening for taste bud-specific genes using DNA micro array .  The Japanese journal of taste and smell research9 ( 3 ) 319 - 320   2002.12Screening for taste bud-specific genes using DNA micro array

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    Language:Japanese   Publisher:The Japanese Association for the Study of Taste and Smell  

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  • KIM Mi-Ryung, KUSAKABE Yuko, MIURA Hirohito, SHINDO Yoichiro, NINOMIYA Yuzo, HINO Akihiro .  Expression patterns of T1R3 and various taste perception related genes .  The Japanese journal of taste and smell research9 ( 3 ) 659 - 660   2002.12Expression patterns of T1R3 and various taste perception related genes

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    Language:Japanese   Publisher:The Japanese Association for the Study of Taste and Smell  

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  • 進藤洋一郎,日下部裕子,金美令,橋本リカ,田上みづほ,加藤ひろみ,三浦裕仁,日野明寛 .  DNAマイクロアレイを用いた味受容関連遺伝子の網羅的検索 .  日本味と匂学会誌9 ( 3 ) 319 - 320   2002.12DNAマイクロアレイを用いた味受容関連遺伝子の網羅的検索Reviewed

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  • 三浦裕仁,日下部裕子,二ノ宮裕三,元山純,加藤ひろみ,田上みづほ,日野明寛 .  成体マウスにおける味神経に依存した味蕾の維持に関する解析 .  日本味と匂学会誌9 ( 3 ) 605 - 606   2002.12成体マウスにおける味神経に依存した味蕾の維持に関する解析Reviewed

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  • K Kitamura, M Yanazawa, N Sugiyama, H Miura, A Iizuka-Kogo, M Kusaka, K Omichi, R Suzuki, Y Kato-Fukui, K Kamiirisa, M Matsuo, S Kamijo, M Kasahara, H Yoshioka, T Ogata, T Fukuda, Kondo, I, M Kato, WB Dobyns, M Yokoyama, K Morohashi .  Mutation of ARX causes abnormal development of forebrain and testes in mice and X-linked lissencephaly with abnormal genitalia in humans .  NATURE GENETICS32 ( 3 ) 359 - 369   2002.11

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    Publisher:NATURE AMERICA INC  

    Male embryonic mice with mutations in the X-linked aristaless-related homeobox gene (Arx) developed with small brains due to suppressed proliferation and regional deficiencies in the forebrain. These mice also showed aberrant migration and differentiation of interneurons containing gamma-aminobutyric acid (GABAergic interneurons) in the ganglionic eminence and neocortex as well as abnormal testicular differentiation. These characteristics recapitulate some of the clinical features of X-linked lissencephaly with abnormal genitalia (XLAG) in humans. We found multiple loss-of-function mutations in ARX in individuals affected with XLAG and in some female relatives, and conclude that mutation of ARX causes XLAG. The present report is, to our knowledge, the first to use phenotypic analysis of a knockout mouse to identify a gene associated with an X-linked human brain malformation.

    DOI: 10.1038/ng1009

    Web of Science

  • Y Kusakabe, H Miura, R Hashimoto, C Sugiyama, Y Ninomiya, A Hino .  The neural differentiation gene Mash-1 has a distinct pattern of expression from the taste reception-related genes gustducin and T1R2 in the taste buds .  CHEMICAL SENSES27 ( 5 ) 445 - 451   2002.6

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    Publisher:OXFORD UNIV PRESS  

    Taste bud cells have a limited lifespan and are continuously replaced just like other epithelial cells. Although there is some evidence that taste buds may arise from the local epithelium, taste receptor cells have neuronal properties. This implies that there must be a critical stage at which the epithelial precursor cells for taste receptor cells start to exhibit neural properties during the differentiation of the taste receptor cells. The expression of the neural-specific transcription factors Mash-1 and Prox-1 in the nervous system is transient and precedes neuronal differentiation. Therefore, we examined the expression of Mash-1 and Prox-1 in the epithelium of circumvallate papillae of the tongue in order to clarify the localization of the precursor cells with neural properties and observed that both expressions are restricted to the taste buds. Two-colour in situ hybridization showed that the signals for Mash-1 did not overlap those for taste receptor cell-specific genes such as gustducin and T1R2. In the process of development and regeneration of the taste buds, the expression of Mash-1 preceded that of gustducin and T1R2. These observations suggest that Mash-1 could be a candidate for a marker of immature taste receptor cells, including the cells that express gustducin and/or T1R2 at a later stage.

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  • Matsuoka T,Kuribara H,Takubo K,Akiyama H,Miura H,Goda Y,Kusakabe Y,Isshiki K,Toyoda M,Hino A .  Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays). .  J. Agric. Food. Chem.50 ( 7 ) 2100 - 2109   2002.3Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays).Reviewed

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  • KUSAKABE Yuko, MIURA Hirohito, HASHIMOTO Rika, SUGIYAMA Chiaki, NINOMIYA Yuzo, HINO Akihiro .  Comparison of the expression of Mash-I with tast reception-related genes,gustducin and TIR2,in taste buds .  The Japanese journal of taste and smell research8 ( 3 ) 663 - 664   2001.12Comparison of the expression of Mash-I with tast reception-related genes,gustducin and TIR2,in taste budsReviewed

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  • KITAGAWA Michinori, KUSAKABE Yuko, MIURA Hirohito, NINOMIYA Yuzo, HINO Akihiro .  Molecular Genetic Identification of Novel Receptor Gene TIR3 .  The Japanese journal of taste and smell research8 ( 3 ) 303 - 304   2001.12Molecular Genetic Identification of Novel Receptor Gene TIR3Reviewed

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    Language:Japanese   Publisher:The Japanese Association for the Study of Taste and Smell  

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  • MI-RYUNG Kim, KUSAKABE Yuko, MIURA Hirohito, HASHIMOTO Rika, TAGAMI Mizuho, NINOMIYA Yuzo, HINO Akihiro .  Construction of the subtracted cDNA library for gene expression profiling of the taste bud .  The Japanese journal of taste and smell research8 ( 3 ) 651 - 652   2001.12Construction of the subtracted cDNA library for gene expression profiling of the taste budReviewed

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    Language:Japanese   Publisher:The Japanese Association for the Study of Taste and Smell  

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  • MIURA Hirohito, KUSAKABE Yuko, NINOMIYA Yuzo, MOTOYAMA Jun, HINO Akihiro .  Shh and Ptc are Assoviated with Bud Maintenance in the Adult Mouse .  The Japanese journal of taste and smell research8 ( 3 ) 665 - 666   2001.12Shh and Ptc are Assoviated with Bud Maintenance in the Adult MouseReviewed

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    Authorship:Lead author   Language:Japanese   Publisher:The Japanese Association for the Study of Taste and Smell  

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  • SHIGEMURA Noriatsu, MIURA Hirohito, HINO Akihiro, NINOMIYA YUzo .  Expression of leptin peceptors (Ob-rs) and STAT3 mRNA in the mouse taste buds .  The Japanese journal of taste and smell research8 ( 3 ) 627 - 630   2001.12Reviewed

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    Language:Japanese   Publisher:The Japanese Association for the Study of Taste and Smell  

    CiNii Books

  • S Sato, M Tanaka, H Miura, K Ikeo, T Gojobori, T Takeuchi, H Yamamoto .  Functional conservation of the promoter regions of vertebrate tyrosinase genes .  JOURNAL OF INVESTIGATIVE DERMATOLOGY SYMPOSIUM PROCEEDINGS6 ( 1 ) 10 - 18   2001.11Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BLACKWELL PUBLISHING INC  

    Tyrosinase is the key enzyme for synthesizing melanin pigments, which primarily determine mammalian skin coloration. Considering the important roles of pigments in the evolution and the adaptation of vertebrates, phylogenetic changes in the coding and flanking regulatory sequences of the tyrosinase gene are particularly intriguing. We have now cloned cDNA encoding tyrosinase from Japanese quail and snapping turtle. These nonmammalian cDNA are highly homologous to those of the mouse and human tyrosinases, whereas the 5' flanking sequences are far less conserved except for a few short sequence motifs. Nevertheless, we demonstrate that the 5' flanking sequences from the quail or turtle tyrosinase genes are capable of directing the expression of a fused mouse tyrosinase cDNA when introduced into cultured mouse albino melanocytes. This experimental method, which reveals the functional conservation of regulatory sequences in one cell type (the melanocyte), may be utilized to evaluate phylogenetic differences in mechanisms controlling specific gene expression in many other types of cells. We also provide evidence that the 5' flanking sequences from these nonmammalian genes are functional in vivo by producing transgenic mice. Phylogenetic changes of vertebrate tyrosinase promoters and the possible involvement of conserved sequence motifs in melanocyte-specific expression of tyrosinase are discussed.

    Web of Science

  • 日下部裕子、三浦裕仁、橋本リカ、杉山千秋、二ノ宮裕三、日野明寛 .  神経分化マーカーMash-1と味覚情報伝達関連遺伝子gustducin,T1R2の発現様式の比較 .  日本味と匂学会誌8 ( 2 ) 663 - 664   2001.8神経分化マーカーMash-1と味覚情報伝達関連遺伝子gustducin,T1R2の発現様式の比較Reviewed

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    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

  • 北川道憲、日下部裕子、三浦裕仁、二ノ宮裕三、日野明寛 .  甘味受容体T1R3のクローニングと発現の解析 .  日本味の匂学会誌8 ( 2 ) 303 - 304   2001.8甘味受容体T1R3のクローニングと発現の解析Reviewed

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    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

  • 金美令、日下部裕子、三浦裕仁、橋本リカ、田上みづほ、二ノ宮裕三、日野明寛 .  有郭乳頭頭上皮由来ライブラリーの部分プロファイリング .  日本味と匂学会誌8 ( 2 ) 651 - 652   2001.8有郭乳頭頭上皮由来ライブラリーの部分プロファイリングReviewed

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    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

  • H Miura, Y Kusakabe, C Sugiyama, M Kawamatsu, Y Ninomiya, J Motoyama, A Hino .  Shh and Ptc are associated with taste bud maintenance in the adult mouse .  MECHANISMS OF DEVELOPMENT106 ( 1-2 ) 143 - 145   2001.8

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    Publisher:ELSEVIER SCIENCE BV  

    In mammals, taste receptor cells are organized into taste buds on tongue. Taste buds are trophically maintained by taste neurons and under continuous renewal, even in adults. We found that the receptor for Sonic hedgehog (Shh), Patched1 (Ptc), was expressed around taste buds where cells were proliferating, and that Shh was expressed within basal cells of taste buds. Denervation caused the loss of Shh and Ptc expression before the degeneration of taste buds. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

    DOI: 10.1016/S0925-4773(01)00414-2

    Web of Science

  • M Kitagawa, Y Kusakabe, H Miura, Y Ninomiya, A Hino .  Molecular genetic identification of a candidate receptor gene for sweet taste .  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS283 ( 1 ) 236 - 242   2001.4

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    Publisher:ACADEMIC PRESS INC  

    A cDNA clone encoding a novel member of the putative taste receptor T1R family, designated T1R3, was isolated from circumvallate papillae of the mouse tongue using degenerate primers. Reverse transcription-polymerase chain reaction analysis showed predominant expression of the receptor in circumvallate papillae. In situ hybridization analysis revealed that T1R3 was expressed in a subset of taste receptor cells in taste buds and that the topographic distribution of T1R3 in various taste papillae was different from those of the other T1R members. Genetic mapping of T1R3 with a mouse/hamster radiation hybrid panel located the gene on the distal end of mouse chromosome 4 correlated with the Sac locus affecting sweet sensitivity of mice. Our results indicate that T1R3 may serve as the receptor for sweet perception in mice. (C) 2001 Academic Press.

    DOI: 10.1006/bbrc.2001.4760

    Web of Science

  • MATSUOKA Takeshi, KURIBARA Hideo, AKIYAMA Hiroshi, MIURA Hirohito, GODA Yukihiro, KUSAKABE Yuko, ISSHIKI Kenji, TOYODA Masatake, HINO Akihiro .  A Multiplex PCR Method f Detecting Recombinant DNAs from Five Lines of Genetically Modified Maize .  Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)42 ( 1 ) 24 - 32   2001.2

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    Publisher:Japanese Society for Food Hygiene and Safety  

    Seven lines of genetically modified (GM) maize have been authorized in Japan as foods and feeds imported from the USA. We improved a multiplex PCR method described in the previous report in order to distinguish the five lines of GM maize. Genomic DNA was extracted from GM maize with a silica spin column kit, which could reduce experimental time and improve safety in the laboratory and potentially in the environment. We sequenced recombinant DNA (r-DNA) introduced into GM maize, and re-designed new primer pairs to increase the specificity of PCR to distinguish five lines of GM maize by multiplex PCR. A primer pair for the maize intrinsic <i>zein</i> gene (<i>Ze1</i>) was also designed to confirm the presence of amplifiable maize DNA. The lengths of PCR products using these six primer pairs were different. The <i>Ze1</i> and the r-DNAs from the five lines of GM maize were qualitatively detected in one tube. The specific PCR bands were distinguishable from each other on the basis of the expected length. The r-DNA could be detected from maize samples containing 0.5% of each of the five lines of GM maize. The sensitivity would be acceptable to secure the verification of non-GMO materials and to monitor the reliability of the labeling system.

    DOI: 10.3358/shokueishi.42.24

    Web of Science

    CiNii Books

    Other Link: https://agriknowledge.affrc.go.jp/RN/2010621847

  • SHIGEMURA Noriatsu, MIURA Hirohito, KUSAKABE Yuko, HINO Akihiro, NAKASHIMA Kiyohito, SUGIMOTO Kumiko, NINOMIYA Yuzo .  Expression of receptors (Ob-Rb) and sweet suppressive effects of leptin in the mouse taste buds .  The Japanese journal of taste and smell research7 ( 3 ) 523 - 536   2000.12Reviewed

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    Language:Japanese   Publisher:The Japanese Association for the Study of Taste and Smell  

    CiNii Books

  • K Kawai, K Sugimoto, K Nakashima, H Miura, Y Ninomiya .  Leptin as a modulator of sweet taste sensitivities in mice .  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA97 ( 20 ) 11044 - 11049   2000.9

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    Publisher:NATL ACAD SCIENCES  

    Leptin acts as a potent inhibitory factor against obesity by regulating energy expenditure, food intake, and adiposity. The obese diabetic db/db mouse, which has defects in leptin receptor, displays enhanced neural responses and elevated behavioral preference to sweet stimuli. Here, we show the effects of leptin on the peripheral taste system. An administration of leptin into lean mice suppressed responses of peripheral taste nerves (chorda tympani and glossopharyngeal) to sweet substances (sucrose and saccharin) without affecting responses to sour, salty, and bitter substances. Whole-cell patch-clamp recordings of activities of taste receptor cells isolated from circumvallate papillae (innervated by the glossopharyngeal nerve) demonstrated that leptin activated outward K+ currents, which resulted in hyperpolarization of taste cells. The db/db mouse with impaired leptin receptors showed no such leptin suppression. Taste tissue (circumvallate papilla) of lean mice expressed leptin-receptor mRNA and some of the taste cells exhibited immunoreactivities to antibodies of the leptin receptor. Taken together, these observations suggest that the taste organ is a peripheral target for leptin, and that leptin may be a sweet-sensing modulator (suppressor) that may take part in regulation of food intake. Defects in this leptin suppression system in db/db mice may lead to their enhanced peripheral neural responses and enhanced behavioral preferences for sweet substances.

    DOI: 10.1073/pnas.190066697

    Web of Science

  • T Matsuoka, Y Kawashima, H Akiyama, H Miura, Y Goda, Y Kusakabe, K Isshiki, M Toyoda, A Hino .  A method of detecting recombinant DNAs from four lines of genetically modified maize .  JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN41 ( 2 ) 137 - 143   2000.4Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FOOD HYG SOC JPN  

    A method using polymerase chain reaction (PCR) was designed for the detection of genetically modified maize (GM-maize). There are four lines of GM-maize imported from the United States, and the presence of recombinant deoxyribonucleic acid (DNA) in the maize could be detected with four pairs of specific oligonucleotide primers designed from the sequences of the newly introduced genes. The maize zein gene was also detected as an internal control. This method allows specific detection of each of Bt11, Event176, MON810 and LIBERTY by using pairs of specific primers designed to amplify a segment including part: of the exogenously introduced sequence and part of the intrinsic maize sequence. The detection sensitivity was about 0.05% for Event176, MON810 and LIBERTY, and about 0.01% for Bt11. To distinguish among three insect-resistant GM-maize lines, we designed a multiplex PCR method. These three GM-maize lines were distinguishable on the basis of the expected lengths of their amplicons.

    DOI: 10.3358/shokueishi.41.137

    Web of Science

  • K Kitamura, H Miura, S Miyagawa-Tomita, M Yanazawa, Y Katoh-Fukui, R Suzuki, H Ohuchi, A Suehiro, Y Motegi, Y Nakahara, S Kondo, M Yokoyama .  Mouse Pitx2 deficiency leads to anomalies of the ventral body wall, heart, extra- and periocular mesoderm and right pulmonary isomerism .  DEVELOPMENT126 ( 24 ) 5749 - 5758   1999.12

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    Publisher:COMPANY OF BIOLOGISTS LTD  

    Pitx2, a bicoid-related homeobox gene, is involved in Rieger's syndrome and the left-right (L-R) asymmetrical pattern formation in body plan. In order to define the genomic structure and roles of Pitx2, we analyzed the genomic structure and generated Pitx2-deficient mice with the lacZ gene in the homeobox-containing exon of Pitx2, We were able to show that among three isoforms of Pitx2, Pitx2c shows asymmetrical expression whereas Pitx2a, Pitx2b and Pitx2c show symmetrical expression. In Pitx2(-/-) embryos there was an increase in mesodermal cells in the distal end of the left lateral body wall and an amnion continuous with the lateral body wall thickened in its mesodermal layer These changes resulted in a failure of ventral body wall closure. In lung and heart in which Pitx2 is expressed asymmetrically, right pulmonary isomerism, atrioventricular canals with prominent swelling, and juxtaposition of the atrium were detected. The hearts failed to develop tricuspid and mitral valves and a common atrioventricular valve forms. Further, dysgenesis of the Pitx2(-/-) extraocular muscle and thickening of the mesothelial layer of cornea were observed in the ocular system where Pitx2 is expressed symmetrically, and these resulted in enophthalmos. The present study shows that Pitx2 expressed in various sites participates in morphogenesis through three types of actions: the involvement of asymmetric Pitx2, expression in the entire morphogenetic process of L-R asymmetric organs; the involvement of asymmetric Pitx2 expression in the regional morphogenesis of asymmetric organs; and finally the involvement of symmetric Pitx2 expression in the regional morphogenesis of symmetric organs.

    Web of Science

  • K Kitamura, H Miura, M Yanazawa, T Miyashita, K Kato .  Expression patterns of Brx1 (Rieg gene), Sonic hedgehog, Nkx2.2, Dlx1 and Arx during zona limitans intrathalamica and embryonic ventral lateral geniculate nuclear formation .  MECHANISMS OF DEVELOPMENT67 ( 1 ) 83 - 96   1997.9Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    The Brx1 homeobox gene has been isolated and shown to be expressed in the zona limitans intrathalamica (ZLI) of the mouse embryo. Brx1 is a member of the Err gene family and comprises the genes for Brx1a and Brx1b, which differ in the sequence in the region located on the 5'-terminal side of the homeobox. The complete amino acid sequences of the open reading frame of Brx1a and Brx1b were determined and each was found to be similar to that of Rgs, the mouse homologue of the Rieger syndrome associated human RIEG gene (RGS), to the extent that the sequence of Rgs has been clarified. Brx1 was strongly expressed in the mammillary area as well as in the ZLI of the mouse embryonic brain. Homologues of Brx1a and Brx1b were isolated in chick in which the expression of Brx1 in the ventral diencephalon was well conserved. The expression of Brx1 along with that of Sonic hedgehog (Shh), Nkx2.2, Dlx1 and Arx was examined at the time of the formation of ZLI in mouse embryos. The expression of Shh was initially noted in the ventricular zone of the presumptive ZLI and was then replaced by that of Brx1 at the time of radial migration of the neuroepithelial cells. Nkx2.2 was widely expressed in the ventricular zone of presumptive ZLI and also as a narrow band in the mantle zone. The expression of Dlx1 and Arx in the presumptive ventral thalamus extended as far as ZLI and overlapped with that of Brx1. The Dlx1- and Arx-expressing cells in ZLI, which extended towards the lateral (pial) surface of the diencephalic wall, differed from those expressing Nkx2.2 and Brx1. The embryonic ventral lateral geniculate nucleus present in the visual pathway was eventually formed from these cells. Each homeobox gene was also expressed regionally in the nucleus, suggesting that the nucleus is comprised of subdivisions. (C) 1997 Elsevier Science Ireland Ltd.

    DOI: 10.1016/S0925-4773(97)00110-X

    Web of Science

  • H Miura, M Yanazawa, K Kato, K Kitamura .  Expression of a novel aristaless related homeobox gene 'Arx' in the vertebrate telencephalon, diencephalon and floor plate .  MECHANISMS OF DEVELOPMENT65 ( 1-2 ) 99 - 109   1997.7Reviewed International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    We have isolated a novel homeobox gene that is expressed in the vertebrate central nervous system and which shows striking similarity to the Drosophila al gene in the homeodomain (85% identity) and in a 17 amine acid-sequence near the carboxyl-terminus. This gene was designated Arx (aristaless related homeobox gene) in consideration of its structural similarity to the al gene. Arx was highly conserved between mouse and zebrafish. Neuromeric expression in the forebrain and longitudinal expression in the floor plate were observed in mouse and zebrafish. The expression of Air in the ganglionic eminence and ventral thalamus overlapped regionally with that of Dlx1, but the cell layer where Acv is expressed differed from that of the Dlx1. This gene was also found to be expressed in the dorsal telencephalon (presumptive cerebral cortex) of mouse embryos. The structure and expression pattern of Arx with respect to any possible relationship to al and Dlx1, as well as the function of Arx in the floor plate are discussed. (C) 1997 Elsevier Science Ireland Ltd.

    DOI: 10.1016/S0925-4773(97)00062-2

    Web of Science

  • S SATO, H MIURA, H YAMAMOTO, T TAKEUCHI .  IDENTIFICATION OF NUCLEAR FACTORS THAT BIND TO THE MOUSE TYROSINASE GENE REGULATORY REGION .  PIGMENT CELL RESEARCH7 ( 5 ) 279 - 284   1994.10

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    Publisher:MUNKSGAARD INT PUBL LTD  

    Several nuclear factors that interact with sequences in the 5' flanking region of the mouse tyrosinase gene were identified using band shift and methylation interference assays. One of these factors bind to an AT-rich sequence, TATCAATTAG, located at -183 base pairs upstream of the transcription start site. To isolate cDNA clone encoding this DNA binding protein, we have screened a lambda gtll cDNA expression library prepared from mouse melanocyte cell line with a labeled oligonucleotide probe containing its binding site. Complementary DNA clones encoding mouse high mobility group protein HMG-I and its isoform HMG-Y were obtained. HMG-I(Y) is a low molecular size, basic nuclear protein that binds specifically to AT-rich region of double-stranded DNA in vitro. In Northern blot analysis the level of HMG-I(Y) mRNA expression did not correlate with that of tyrosinase or TRP-1. Although the amount of HMG-I(Y) transcripts has no apparent influence on the mouse tyrosinase gene expression, it is possible that HMG-I(Y) binds to the 5' flanking sequence of the tyrosinase gene as an auxiliary factor, and facilitates the binding and activity of other transcription factors.

    DOI: 10.1111/j.1600-0749.1994.tb00628.x

    Web of Science

  • H YAMAMOTO, T KUDO, N MASUKO, H MIURA, S SATO, M TANAKA, S TANAKA, S TAKEUCHI, S SHIBAHARA, T TAKEUCHI .  PHYLOGENY OF REGULATORY REGIONS OF VERTEBRATE TYROSINASE GENES .  PIGMENT CELL RESEARCH5 ( 5 ) 284 - 294   1992.11

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    Publisher:MUNKSGAARD INT PUBL LTD  

    Highly homologous DNA elements were found to be shared by the upstream regions of the mouse tyrosinase and tyrosinase related protein (TRP-1) genes. Several nuclear proteins were shown to bind to both of these upstream regions. Shared homologous DNA elements were also found in the 5' flanking sequences of Japanese quail and snapping turtle tyrosinase genes. Shared homologous nucleotide sequences were found to be scattered like an archipelago in the 5' upstream regions of mouse and human tyrosinase genes. Comparisons between Japanese quail and snapping turtle tyrosinase genes gave similar results. On the contrary, mammalian (mouse and human) and nonmammalian (quail and snapping turtle) tyrosinase genes did not show significant homology in their 5' upstream regions. In contrast, coding sequences in the first exons of vertebrate tyrosinase genes and their deduced amino acid sequences were found to be highly conserved except for their putative leader sequence-coding regions.

    DOI: 10.1111/j.1600-0749.1992.tb00551.x

    Web of Science

  • Y Kusakabe, Y Shindo, MR Kim, H Miura, Y Ninomiya, A Hino .  CDNA microarray screening for taste-bud-specific genes .  CHEMICAL SENSES30   I12 - i13   2005.1Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    DOI: 10.1093/chemse/bjh088

    Web of Science

  • T Matsuoka, H Kuribara, K Takubo, H Akiyama, H Miura, Y Goda, Y Kusakabe, K Isshiki, M Toyoda, A Hino .  Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays) .  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY50 ( 7 ) 2100 - 2109   2002.3Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER CHEMICAL SOC  

    Polymerase Chain Reaction (PCR) techniques are increasingly used for the detection of genetically modified (GM) crops in foods. In this paper, recombinant DNAs introduced into the seven lines of GM maize, such as Event 176, Bt11, T25, MON810, GA21, DLL25, and MON802, are sequenced. On the basis of the obtained sequence, 14 primer pairs for the detection of the segments, such as promoter, terminator regions, and construct genes, were designed. To confirm the specificities of the designed primer pairs, PCR was performed on genomic DNAs extracted from GM and non-GM maize, GM and non-GM soy, and other cereal crops. Because the presence of the corresponding DNA segments was specifically detected in GM crops by the designed primer pairs, it was concluded that this method is useful for fast and easy screening of GM crops including unauthorized ones.

    DOI: 10.1021/jf011157t

    Web of Science

  • M TANAKA, S TANAKA, H MIURA, H YAMAMOTO, H KIKUCHI, T TAKEUCHI .  CONSERVED REGULATORY MECHANISMS OF TYROSINASE GENES IN MICE AND HUMANS .  PIGMENT CELL RESEARCH5 ( 5 ) 304 - 311   1992.11Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MUNKSGAARD INT PUBL LTD  

    In vertebrates, melanin production is restricted to pigment cells. This cell type-specific melanogenesis is considered to involve cell type-specific expression of the tyrosinase gene. Recently, there have been several reports that sequences in the 5' flanking region of the mouse tyrosinase gene are responsible for cell type-specific expression of the transgene in mice. As the first step in the study of the evolution of the regulatory mechanisms for tyrosinase gene function in vertebrates, we constructed a fused gene, hg-Tyrs-J, which includes a 1.0-kb 5' flanking sequence of the human tyrosinase gene fused with mouse tyrosinase cDNA. By introducing the fused gene into fertilized eggs of albino mice, we obtained two mice that exhibited pigmentation in the skin and eyes and established a transgenic line from one of them. Further analyses revealed that the transgene was expressed cell type-specifically in these transgenic mice. We conclude, therefore, that the 1.0 kb 5' upstream region of the human tyrosinase gene contains conserved cis-elements essential for cell type-specific expression of the tyrosinase genes in mice and humans. Results of our study may provide a clue to elucidate the evolutionary process of regulatory mechanisms of the tyrosinase gene.

    DOI: 10.1111/j.1600-0749.1992.tb00554.x

    Web of Science

  • H KIKUCHI, H MIURA, H YAMAMOTO, T TAKEUCHI, T DEI, M WATANABE .  CHARACTERISTIC SEQUENCES IN THE UPSTREAM REGION OF THE HUMAN TYROSINASE GENE .  BIOCHIMICA ET BIOPHYSICA ACTA1009 ( 3 ) 283 - 286   1989.12Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    DOI: 10.1016/0167-4781(89)90115-2

    Web of Science

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Books

  • 研究分野紹介ー口腔生理学分野ー

    三浦裕仁、中山歩、大木誠、岩松多美子、原田秀逸( Role: Joint author)

    鹿児島大学歯学部紀要  2013.3 

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    Language:Japanese Book type:Scholarly book

  • 食品大百科事典

    食品総合研究所(感覚機能成分)

    朝倉書店  2001.11  ( ISBN:4254430787

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    Total pages:xxi, 1045p  

    CiNii Books

  • 食品機能研究法

    篠原, 和毅, 鈴木, 建夫, 上野川, 修一(味蕾を含む舌上皮で発現する遺伝子のRT-PCRによる解析法)

    光琳  2000.5  ( ISBN:4771200041

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    Total pages:[8], 348p  

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MISC

  • 味覚の新たな方向性 味蕾細胞分化の調節因子

    三浦 裕仁, 小柳 江梨子, 原田 秀逸

    Journal of Oral Biosciences Supplement   2019   44 - 44   2019.10

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    Publisher:(一社)歯科基礎医学会  

  • 味神経切断が塩酸キニーネに対する忌避行動に及ぼす影響

    大木 誠, 三浦 裕仁, 齋藤 充

    Journal of Oral Biosciences Supplement   2016   331 - 331   2016.9

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    Publisher:(一社)歯科基礎医学会  

  • 巻頭言「本物を味わう」

    三浦裕仁

    日本味と匂学会誌 = The Japanese journal of taste and smell research   23 ( 1 )   1 - 2   2016.4

  • Changes of gustatory preference with age in the rat

    35 ( 35 )   11 - 19   2015.3

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    The effect of aging on taste were compared between the preference and neural responses from the greater superficial petrosal nerve (GSP) innervating the soft palate and the chorda tympani nerve (CT) innervating the fungiform papillae in the young (5-12 weeks) and aged (19-22 months) Sprague Dawley rat. A two-bottle preference test revealed that younger rats significantly preferred 0.001 M 5'-inosine monophosphate (IMP), 0.01 M mono sodium glutamate (MSG), and binary mixtures of 0.001 M IMP + 0.01 M MSG than deionized water. However, aged rats showed no significant preference to these umami solutions. Among the other four basic taste stimuli, there was no significant difference in preference between young and aged rats. Regardless of the age of the rat, neural responses from the GSP and CT produced robust integrated responses to all three umami solutions the same as used in the two-bottle test. These results indicate that the lack of preference to umami in aged rats is occurred in the central nervous system, suggesting that the loss of preference to umami taste in aged rats may be caused by homeostatic changes in the brain.

    CiNii Books

    Other Link: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2015&ichushi_jid=J00245&link_issn=&doc_id=20150522030002&doc_link_id=http%3A%2F%2Fhdl.handle.net%2F10232%2F26722&url=http%3A%2F%2Fhdl.handle.net%2F10232%2F26722&type=%8E%AD%8E%99%93%87%91%E5%8Aw%81F%8E%AD%8E%99%93%87%91%E5%8Aw%83%8A%83%7C%83W%83g%83%8A&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F80042_3.gif

  • 【味の不思議】味蕾を構成する細胞とその分化

    三浦 裕仁

    JOHNS   29 ( 1 )   22 - 25   2013.1

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    Publisher:(株)東京医学社  

  • 味覚研究の伝統の継承と次なる一歩を目指して 味の発生と伝達 味蕾の細胞分化と機能の部位特性

    三浦 裕仁, 友成 博, 中山 歩, 大木 誠, 原田 秀逸

    Journal of Oral Biosciences   53 ( Suppl. )   78 - 78   2011.9

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    Publisher:(一社)歯科基礎医学会  

  • 書評:官能評価データの分散分析 パネルを使った実験の計画から解析まで, リー・ナース・ロズボッテン著, 内田治・秋田カオリ訳, A5判, 200頁, 2,800円+税, 東京図書, 東京, 2010年11月, ISBN978-4-489-02086-5

    三浦 裕仁

    日本味と匂学会誌   18 ( 2 )   148 - 149   2011.8

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    Language:Japanese   Publishing type:Book review, literature introduction, etc.   Publisher:日本味と匂学会  

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  • 総説特集 味蕾培養系を用いた味蕾細胞分化メカニズム解明の試み-1特集にあたって Reviewed

    三浦裕仁、宮本武典

    日本味と匂学会誌   18 ( 1 )   5 - 6   2011.4

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • コロラド滞在記

    三浦 裕仁

    日本味と匂学会誌 = The Japanese journal of taste and smell research   17 ( 2 )   136 - 137   2010.8

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    Language:Japanese   Publisher:日本味と匂学会  

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  • Taste bud regeneration and the search for taste progenitor cells Reviewed

    Miura H, Barlow L

    Arch Ital Biol.   148 ( 2 )   107 - 118   2010.6

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • Functions of the Gustducin in the Soft Palate Taste Buds differs from those in the Tongue in Mammals International journal

    Hiroshi Tomonari, Hirohito Miura, Ayumi Nakayama, Eriko Matsumura, Yoichiro Shindo, Yuko Kusakabe, Robert F. Margolskee, Yuzo Ninomiya, Shuitsu Harada

    CHEMICAL SENSES   34 ( 2 )   J10 - J10   2009.2

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    Web of Science

  • 味蕾を維持する分子機構 : 2007年日本味と匂学会研究奨励賞受賞

    三浦 裕仁

    鹿児島大学歯学部紀要   ( 29 )   30 - 31   2009

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    Language:Japanese   Publisher:鹿児島大学  

    歯学部における特徴ある研究、診療活動

    CiNii Books

  • Expression of the Basal Cell Markers of Taste Buds during Mouse Embryonic Development International journal

    Hirohito Miura, Ayumi Nakayama, Yoichiro Shindo, Yuko Kusakabe, Hiroshi Tomonari, Shuitsu Harada

    CHEMICAL SENSES   33 ( 8 )   S72 - S73   2008.10

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    Web of Science

  • The Function of the Gustducin in the Soft Palate Taste Buds Differs from that in the Tongue

    Ayumi Nakayama, Hiroshi Tomonari, Hirohito Miura, Yoichiro Shindo, Yuko Kusakabe, Yuzo Ninomiya, Robert F. Margolskee, Shuitsu Harada

    CHEMICAL SENSES   33 ( 8 )   S143 - S143   2008.10

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    Publisher:OXFORD UNIV PRESS  

    Web of Science

  • ラットの大錐体神経と鼓索神経つなぎ換えで再生した味蕾

    中山 歩, 三浦 裕仁, 友成 博, 原田 秀逸

    Journal of Oral Biosciences   50 ( Suppl. )   146 - 146   2008.9

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    Publisher:(一社)歯科基礎医学会  

  • Expression of FXYD6, a member of na KATPase regulator family in taste buds International journal

    Y. Kusakabe, K. Morishita, Y. Shindo, H. Miura, P. Carninci, J. Kawai, Y. Hayashizaki, A. Hino

    CHEMICAL SENSES   33 ( 2 )   J3 - J3   2008.2

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    Web of Science

  • Timing of differentiation and innervation of the basal cells of taste buds during mouse embryogenesis

    A. Nakayama, H. Miura, H. Kato, Y. Shindo, Y. Kusakabe, A. Hino, H. Tomonari, S. Harada

    CHEMICAL SENSES   33 ( 2 )   J11 - J11   2008.2

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    Web of Science

  • マウス発生過程における味蕾基底細胞の分布パターンの形成と神経支配のタイミング

    中山 歩, 三浦 裕仁, 友成 博, 原田 秀逸

    Journal of Oral Biosciences   49 ( Suppl. )   118 - 118   2007.8

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  • ラット軟口蓋の味蕾におけるIP3R3とgustducinの共発現

    三浦 裕仁, 中山 歩, 友成 博, 原田 秀逸

    Journal of Oral Biosciences   49 ( Suppl. )   174 - 174   2007.8

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  • Cell lineage and differentiation in taste bubs. Reviewed

    Miura H,Kusakabe Y,Harada S

    Arch. Histol. Cytol.   69   209 - 225   2006.12

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  • マウスの胎生期における味蕾基底細胞マーカー遺伝子の発現解析

    中山 歩, 三浦 裕仁, 友成 博, 原田 秀逸

    Journal of Oral Biosciences   48 ( Suppl. )   133 - 133   2006.9

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  • 味覚の受容と味蕾細胞分化の分子メカニズム

    三浦 裕仁

    鹿児島大学歯学部紀要   26   27 - 37   2006.3

  • Expression of the marker genes for the basal cells of taste buds during mouse embryogenesis International journal

    A Nakayama, H Miura, H Kato, Y Kusakabe, A Hino, S Harada

    CHEMICAL SENSES   31 ( 1 )   J10 - J10   2006.1

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    Web of Science

  • Identification of novel taste bud-specific genes and their functions in gustatory sensation

    Y Kusakabe, Y Shindo, H Miura, K Mi-Ryung, M Shimizu, Y Ninomiya, A Hino

    CHEMICAL SENSES   31 ( 1 )   J2 - J2   2006.1

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    Publisher:OXFORD UNIV PRESS  

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  • The expression of type III cell marker NCAM and possible cell lineage relationships in mouse taste buds

    H Miura, H Kato, Y Kusakabe, A Nakayama, M Kariatsumari, Y Ninomiya, A Hino, S Harada

    CHEMICAL SENSES   31 ( 1 )   J10 - J10   2006.1

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    Publisher:OXFORD UNIV PRESS  

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  • マウス味蕾におけるNCAMの一過性発現と細胞系譜

    三浦 裕仁, 中山 歩, 原田 秀逸

    Journal of Oral Biosciences   47 ( Suppl. )   176 - 176   2005.9

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  • 味を感じるメカニズム--甘味・うま味・苦味:T1r,T2r受容体を中心として

    三浦 裕仁, 日野 明寛

    ニューフードインダストリー   47 ( 3 )   35 - 44   2005.3

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    Publisher:食品資材研究会  

    CiNii Books

  • わかりはじめた味覚の分子メカニズム

    三浦 裕仁

    食糧   ( 43 )   1 - 16   2005.3

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    Publisher:食品総合研究所  

    CiNii Books

  • 【分子を感じる 感覚の分子生物学】味を感じる:神経メカニズム

    安松 啓子, 三浦 裕仁, 吉田 竜介, 日野 明寛, 二ノ宮 裕三

    細胞工学   21 ( 12 )   1429 - 1433   2002.11

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    Publisher:(株)学研メディカル秀潤社  

    味を受容する味蕾は,味細胞が数十個集まってできている.味細胞の寿命は短く,約10日のサイクルで,新しく置き換わっている(ターンオーバー).味神経は味蕾の基底部にsonic hedgehog(Shh)を誘導し味細胞を増殖し,味蕾を維持するものと推定される.味神経線維は各々適合する特定の味細胞或いはその前駆細胞を選択し,シナプスを作り,ターンオーバーに際しても味の情報を変えることなく脳に伝える

    CiNii Books

  • Shh and Ptc are associated with taste bud maintenance in the adult mouse

    H Miura, Y Kusakabe, Y Ninomiya, J Motoyama, A Hino

    MOLECULAR BIOLOGY OF THE CELL   12   503A - 504A   2001.11

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    Publisher:AMER SOC CELL BIOLOGY  

    Web of Science

  • HPLCによるシキミ酸経路関連酵素の活性測定法の検討

    奥貫 晴代, 都筑 智子, 手島 玲子, 穐山 浩, 合田 幸広, 豊田 正武, 澤田 純一, 荒見 真一郎, 三浦 裕仁, 日野 明寛

    生化学   71 ( 8 )   767 - 767   1999.8

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    Publisher:(公社)日本生化学会  

  • 農作物からの組換え遺伝子の検知法について

    日野 明寛, 松岡 猛, 川島 よしみ, 三浦 裕仁, 一色 賢司, 穐山 浩, 合田 幸広, 豊田 正武

    日本食品衛生学会学術講演会講演要旨集   76回   50 - 50   1998.11

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    Publisher:(公社)日本食品衛生学会  

  • 間脳の分節境界領域とnucleusの形成

    三浦 裕仁

    日本発生生物学会大会講演要旨集   30回   48 - 48   1997.5

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    Publisher:日本発生生物学会  

  • Prosomeric Boundary of Diencephalon and Novel Homeobox Gene ”Mdx1”.

    北村邦夫, 柳沢昌子, 三浦裕仁, 加藤健太郎, 宮下俊雄

    日本発生生物学会大会発表要旨集   29th   186   1996

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  • Cloning of novel bicoid type homeobox gene and its expression in the chick embryo head region.

    柳沢昌子, 宮下俊雄, 三浦裕仁, 北村邦夫

    日本発生生物学会大会発表要旨集   28th   299   1995

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  • Development and maintenance of taste epithelium: Taste buds and surrounding cells International journal

    Hirohito Miura, Ayumi Nakayama, Yuko Kusakabe, Shuitsu Harada

    CHEMICAL SENSES   41 ( 9 )   E228 - E228   2016.11

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    Web of Science

  • Cell Differentiation in the Taste Bud after the Cross-Regeneration between the Chorda Tympani and Greater Superficial Petrosal Nerves International journal

    Ayumi Nakayama, Hiroshi Tomonari, Hirohito Miura, Eriko Matsumura, Shuitsu Harada

    CHEMICAL SENSES   34 ( 2 )   J6 - J7   2009.2

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    Web of Science

  • Cell Differentiation of the Taste Buds on the Soft Palate and Fungiform Papillae Reconnected to Different Gustatory Nerves International journal

    Shuitsu Harada, Hiroshi Tomonari, Ayumi Nakayama, Yoichiro Shindo, Yuko Kusakabe, Hirohito Miura

    CHEMICAL SENSES   33 ( 8 )   S71 - S72   2008.10

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    Web of Science

  • Distribution pattern and development of basal cells of taste buds during mouse embryogenesis International journal

    A. Nakayama, H. Miura, H. Kato, Y. Kusakabe, A. Hino, S. Harada

    CHEMICAL SENSES   32 ( 2 )   J19 - J19   2007.2

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    Web of Science

▼display all

Presentations

  • 三浦裕仁, 小栁江梨子, 原田秀逸   味蕾細胞分化の制御機構  

    第62回歯科基礎医学会 メインシンポジウム1 (味覚の新知見:発生から認知、行動表出まで)  2020.9 

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    Event date: 2020.9 - 2020.10

  • 三浦裕仁, 小栁江梨子, 原田秀逸   味蕾細胞分化の調節因子  

    日本解剖学会 第75回歯九州支部学術集会 特別講演  2019.11 

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    Event date: 2019.11

  • 三浦裕仁, 小栁江梨子, 原田秀逸   味蕾細胞分化の調節因子  

    第61回歯科基礎医学会 メインシンポジウム2 (味覚の新たな方向性)  2019.10 

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    Event date: 2019.10 - 2020.10

  • Regulatory factors of taste vell differentiation   Invited

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    Event date: 2019.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

  • Miura H, Koyanagi E, Harada H.   Regulation of taste cell maturation.   International conference

    The 17th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception 

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    Event date: 2018.12

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:福岡  

  • Hirohito Miura, Eriko Koyanagi, Shuitsu Harada   味細胞成熟分化の調節  

    第17回国際シンポジウム“味覚嗅覚の分子神経機構  2018.12 

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    Event date: 2018.11 - 2018.12

  • Miura H.   Development and maintenance of taste epithelium:Taste buds and surrounding cells.   Invited International conference

    Seoul Taste Meeting 2016 

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    Event date: 2016.11

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:韓国、ソウル  

  • Hirohito Miura   Development and maintenance of taste epithelium:Taste buds and surrounding cells   Invited International conference

    Seoul Taste Meeting 2016  2016.11 

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    Event date: 2016.11

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  • Miura H, Nakayama, A, Kusakabe Y, Harada H.   Development and maintenance of taste epithelium: Taste buds and surrounding cells.   International conference

    The 17th International Symposium on Olfaction and Taste 

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    Event date: 2016.6

    Language:English   Presentation type:Poster presentation  

    Venue:横浜  

  • 三浦裕仁、中山歩、Jennifer K. Scott、友成博、大木誠、Linda A. Barlow、原田秀逸   Development of the basal cells in taste buds and taste cell differentiation  

    第91回日本生理学会大会 シンポジウム 「口腔・脳・腸に発現する味覚センサーの部位特異性と機能連関」  第91回日本生理学会大会 シンポジウム 「口腔・脳・腸に発現する味覚センサーの部位特異性と機能連関」

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    Event date: 2014.3

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:鹿児島  

    国内学会

  • Miura H, Scott JK, Nakayama A, Harada S, Barlow LA   Shh-expressing basal cells are immediate precursors of Type I, II and III cells in taste buds.  

    The 9th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (ISMNTOP)  2012.12 

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    Event date: 2012.12

  • Hirohito Miura, Jennifer K. Scott, Ayumi Nakayama, Shuitsu Harada and Linda A. Barlow   Shh-expressing basal cells are immediate precursors of Type I, II and III cells in taste buds.   International conference

    The 10th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory perception  The 10th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory perception

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    Event date: 2012.11

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:福岡  

    国際学会

  • Hirohito Miura, Jennifer K. Scott, Ayumi Nakayama, Shuitsu Harada, Linda A. Barlow.   Shh-expressing basal cells are immediate precursors of Type I, II and III cells in taste buds   International conference

    XVI International Symposium on Olfaction and Taste (ISOT)  XVI International Symposium on Olfaction and Taste (ISOT)

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    Event date: 2012.6

    Language:English  

    Venue:スウェーデン (ストックホルム)  

    国際学会

  • Ayumi Nakayama, Hirohito Miura, Makoto Ooki, Yuko Kusakabe and Shuitsu Harada   Basal cell markers and Sox2 during taste bud development and maturation.   International conference

    XVI International Symposium on Olfaction and Taste (ISOT)  XVI International Symposium on Olfaction and Taste (ISOT)

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    Event date: 2012.6

    Language:English  

    Venue:ストックホルム, スウェーデン  

    国際学会

  • Makoto Ooki, Hiroshi Tomonari, Ayumi Nakayama, Hirohito Miura and Shuitsu Harada.   Regional differences in gustatory responses to bitter substances between the soft palate and fungiform papillae.   International conference

    XVI International Symposium on Olfaction and Taste (ISOT)  XVI International Symposium on Olfaction and Taste (ISOT)

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    Event date: 2012.6

    Language:English  

    Venue:ストックホルム, スウェーデン  

    国際学会

  • Barlow LA, Scott JK, Miura H   Shh-expressing basal cells are immediate precursors of taste receptor cells.  

    The 34 Annual Meeting of Association for Chemoreception Sciences (AChemS), Selected oral presentation  2012.4 

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    Event date: 2012.4

  • Linda A. Barlow, Jennifer K. Scott, Hirohito Miura   Shh-expressing basal cells are immediate precursors of taste receptor cells.   International conference

    Association for Chemoreception Sciences (AChemS): 35th Annual Meeting  Association for Chemoreception Sciences (AChemS): 35th Annual Meeting

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    Event date: 2012.4

    Language:English  

    Venue:ハンチントンビーチ, 米国  

    国際学会

  • Miura H, Scott JK, Barlow LA   Shh-expressing basal cells are immediate precursors of taste receptor cells.   International conference

    Gordon Research Conference, Craniofacial Morphogenesis and Tissue Regeneration  Gordon Research Conference, Craniofacial Morphogenesis and Tissue Regeneration

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    Event date: 2012.3

    Language:English  

    Venue:Ventura, CA, USA  

    国際学会

  • 中山歩、三浦裕仁、大木誠、日下部裕子、原田秀逸   味蕾分化・成熟過程における味蕾基底細胞マーカーとSox2の発現(Basal cell markers of taste buds and Sox2 during taste bud development and maturation)   International conference

    第9回国際シンポジウム「味覚・嗅覚の分子神経機構」(The 9th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception)  第9回国際シンポジウム「味覚・嗅覚の分子神経機構」(The 9th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception)

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    Event date: 2011.11

    Language:English  

    Venue:福岡  

    国際学会

  • 三浦裕仁、友成博、中山歩、松村江梨子、大木誠、原田秀逸   軟口蓋と茸状乳頭における苦味情報伝達系の部位差(Regional differences in bitter taste signal transduction between the soft palate and fungiform papillae)   International conference

    第9回国際シンポジウム「味覚・嗅覚の分子神経機構」(The 9th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception)  第9回国際シンポジウム「味覚・嗅覚の分子神経機構」(The 9th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception)

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    Event date: 2011.11

    Language:English  

    Venue:福岡  

    国際学会

  • 大木誠,三浦裕仁,中山歩,ロバート マルゴルスキー,二ノ宮裕三,原田 秀逸   苦味物質に対する応答性および受容機構の口腔内部位差  

    苦味物質に対する応答性および受容機構の口腔内部位差  苦味物質に対する応答性および受容機構の口腔内部位差

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    Event date: 2011.10

    Language:Japanese  

    Venue:石川、金沢  

    国内学会

  • 中山歩,三浦裕仁,大木誠,日下部裕子,原田秀逸   味蕾の発生・成熟過程における味蕾基底細胞マーカーとSox2の発現  

    日本味と匂学会第45会大会  日本味と匂学会第45会大会

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    Event date: 2011.10

    Language:Japanese  

    Venue:石川、金沢  

    国内学会

  • 日下部裕子,進藤由美子,河合 崇行,川本 恵子,三浦裕仁   レセプター型チロシンキナーゼc-Kitのマウス味蕾における発現様式  

    日本味と匂学会第45会大会  日本味と匂学会第45会大会

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    Event date: 2011.10

    Language:Japanese  

    Venue:石川、金沢  

    国内学会

  • 三浦裕仁、友成博、中山歩、原田秀逸   味蕾の細胞分化と機能の部位特性  

    第53回基礎歯科医学会学術大会 サテライトシンポジウム  第53回基礎歯科医学会学術大会 サテライトシンポジウム

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    Event date: 2011.9

    Language:Japanese  

    Venue:岐阜  

    国内学会

  • 三浦裕仁、中山歩、大木誠、原田秀逸   味蕾細胞の発生とターンオーバーにおけるProx1細胞の出現(Appearance of Prox1 cells during taste bud development and turnover)   International conference

    第8回国際シンポジウム「味覚・嗅覚の分子神経機構」(The 8th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception)  第8回国際シンポジウム「味覚・嗅覚の分子神経機構」(The 8th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception)

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    Event date: 2010.10

    Language:English  

    Venue:日本、福岡  

    国際学会

  • 日下部裕子, 進藤洋一郎, 三浦裕仁, カルニンチ ピエロ, 河合純, 林崎良英, 二宮裕三, 日野明寛   味蕾特異的に発現する遺伝子の口腔内の部位による多様な発現様式  

    日本味と匂学会第43回大会 シンポジウム「味覚情報の伝達機構」  2009.9 

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    Event date: 2009.9

  • 日下部裕子、進藤洋一郎、三浦裕仁、カルニンチピエロ、河合純、林崎良英、ニノ宮裕三、日野明寛   味細胞特異的に発現する遺伝子の口腔内の部位による多様な発現様式  

    日本味と匂学会第43回大会シンポジウム[「味覚情報の伝達機構」  日本味と匂学会第43回大会シンポジウム[「味覚情報の伝達機構」

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    Event date: 2009.9

    Language:Japanese  

    Venue:日本、旭川  

    国内学会

  • 三浦 裕仁, 小柳 江梨子, 原田 秀逸   味覚の新知見:発生から認知,行動表出まで 味蕾細胞分化の制御機構  

    Journal of Oral Biosciences Supplement  2020.9  (一社)歯科基礎医学会

  • 三浦 裕仁, 小柳 江梨子, 原田 秀逸   味覚の新たな方向性 味蕾細胞分化の調節因子  

    Journal of Oral Biosciences Supplement  2019.10  (一社)歯科基礎医学会

  • 三浦裕仁   正常な味覚が維持されるしくみ:味蕾細胞のターンオーバー  

    第30回日本産業衛生学会全国協議会 シンポジウム4 (産業歯科保険部会企画)  2020.11 

  • Miura H, Tomonari H, Nakayama A, Matsumura E, Ooki M, Harada S   Regional differences in bitter taste signal transduction between the soft palate and fungiform papillae  

    The 9th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (ISMNTOP)  2011.11 

  • Miura H, Tomonari H, Nakayama A, Matsumura E, Harada S   Regional difference in gustducin and IP3R3 expression in Type II taste bud cells is nerve-independent.  

    The 6th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (ISMNTOP)  2008.12 

  • Shindo Y, Miura H, Carninci P, Kawai J, Hayashizaki Y, Ninomiya Y, Hino A, Kanda T, Kusakabe Y   Gα14 is a candidate mediator of sweet/umami signal transduction in the posterior region of the mouse tongue.  

    The 6th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (ISMNTOP)  2008.12 

  • Miura H, Nakayama A, Shindo Y, Kusakabe Y, Tomonari H, Harada S   Expression of the basal cell marker genes of taste buds in the mouse embryo.   International conference

    The 5th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (ISMNTOP)  2007.11 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  • 日下部裕子, 進藤洋一郎, 三浦裕仁, 二ノ宮裕三, 日野明寛   遺伝子探索による味覚受容機構解明とその「食」への利用.  

    日本農芸化学会2007年度大会 シンポジウム「女性研究者と考える遺伝子テクノフォーラム2007」  2007.3 

  • 日下部裕子, 進藤洋一郎, 三浦裕仁, 金美令, 清水真都香, 二ノ宮裕三, 日野明寛   味覚関連遺伝子の探索とその機能解析  

    日本味と匂学会第39回大会 シンポジウム「味覚の受容から認知にいたるしくみ−最近のトピックス」  2005 

  • 三浦裕仁, 友成博, 中山歩, 原田秀逸   味蕾の細胞分化と機能の部位特性  

    第53回基礎歯科医学会学術大会 サテライトシンポジウム  2011.9 

  • 三浦裕仁, 中山歩, 進藤洋一郎, 日下部裕子, 友成博, 原田秀逸   味蕾における細胞分化と味神経の役割.  

    大阪大学大学院歯学研究科21世紀COEプログラム, COEオープンシンポジウム2006「口腔感覚−痛覚と味覚−」  2006.12 

  • 三浦裕仁, 原田秀逸, 二ノ宮裕三   マウス味蕾における細胞系譜の解析  

    第50回日本顕微鏡学会大会 シンポジウム「味覚・嗅覚受容機構の分子形態学〜顕微鏡で見えるもの」  2005.11 

  • Miura H, Kato H, Kusakabe Y, Tagami M, Miura-Ohnuma J, Ninomiya Y, Hino A   Shh/Ptc signaling and regulatory gene expression in mouse taste bud.  

    The 2nd International Workshop on molecular and Neural Mechanism of Taste Perception  2003.12 

  • Miura H, Kusakabe Y, Ookura T, Ninomiya Y, Hino A   Shh signaling and taste bud maintenance in the adult mouse.  

    The 26th Annual Meeting of the Association for Chemoreception Sciences (AChemS), Symposium [Developmental Regulatory Genes in the Taste and Olfactory Systems]  2004.4 

  • Miura H, Kato H, Kusakabe Y, Tagami M, Miura-ohnuma J, Ookura T, Shindo Y, Ninomiya Y, Hino A   Shh signaling and regulatory gene expression in the mouse taste buds.  

    The 14th International Symposium on Olfaction and Taste (ISOT), Symposium [Development and lineage of taste bud]  2004.7 

  • Kusakabe Y, Kim MR, Miura H, Shindo Y, Ninomiya Y, Hino A   Regional expression patterns of T1r family in the mouse tongue.  

    The 14th International Symposium on Olfaction and Taste (ISOT), Symposium  2004.7 

  • Hirohito Miura, Ayumi Nakayama, Hiroshi Tomonari, Makoto Ooki, Shuitsu Harada   味蕾基底細胞の発生と味細胞分化  

    2014.3 

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    Event date: 2014.3

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  • Miura H, Nakayama A, Tomonari H, Harada S   Differentiation of the basal cells of mouse taste buds.   International conference

    The 4th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (ISMNTOP)  2006.7 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  • Miura H, Nakayama A, Kato H, Kusakabe Y, Hino A, Ninomiya Y, Harada S   Cell lineage relationships and the basal cell differentiation in mouse taste buds.   International conference

    The 3rd International Symposium on Molecular and Neural Mechanism of Taste and Smell (ISMNTOP)  2005.11 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  • Kusakabe Y, Shindo Y, Kim M-R, Miura H, Ninomiya Y, Hino A   cDNA microarray screening for taste bud specific genes.   International conference

    The 14th International Symposium on Olfaction and Taste (ISOT), Symposium[Molecular aspects of mammalian taste signaling]  2004.7 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  • Miura H, Nakayama A, Ooki M, Harada S   Appearance of Prox1 cells during taste bud development and turnover.   International conference

    The 8th International Symposium on Molecular and Neural Mechanism of Taste and Olfactory Perception (ISMNTOP)  2010.11 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

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Awards

  • 日本味と匂学会研究奨励賞

    2007.7  

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    Country:Japan

Research Projects

  • 味蕾基底細胞の再定義:「細胞型分化の解明」と「味蕾オルガノイド評価系の確立」

    Grant number:20K09892  2020.4 - 2023.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    三浦 裕仁, 小柳 江梨子, 原田 秀逸, 山中 淳之

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    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

  • 味蕾細胞分化メカニズムの解明:I,II,III型の枠を越えて味覚機能を制御する転写因子

    2017.4 - 2020.3

    科学研究費補助金  基盤研究(C)

    三浦裕仁、小栁江梨子、大木誠、原田秀逸、日下部裕子

  • 味蕾細胞分化メカニズムの解明:Ⅰ,Ⅱ,Ⅲ型の枠を越えて味覚機能を制御する転写因子

    Grant number:17K11647  2017.4 - 2020.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    三浦 裕仁, 小柳 江梨子, 原田 秀逸, 大木 誠

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    味蕾は、甘味/うま味/苦味/酸味/塩味(5基本味)の味受容細胞を含む数10~100個の細胞が集まった味覚受容器である。味蕾細胞は上皮幹細胞から生み出され、平均すると10~14日という短い寿命で次々と置き換わっている(味蕾細胞のターンオーバー)。健康な状態では、味蕾細胞の新生・分化と細胞死がバランスよく進行し、味蕾に含まれる細胞の数と性質はほぼ一定で、正常な味覚機能が保たれる。味蕾のターンオーバーの異常は味覚障害を引き起こすため、その分子機構の解明は味覚障害の予防法や治療法の開発にたいへん重要である。しかし、その詳細は大部分不明のままである。私達は、これまでに味蕾が形成される最も初期の段階(胎生期の味蕾原基)から、成体の味蕾に至るまで、ほぼ全ての味蕾細胞に発現し続ける転写因子(遺伝子発現の調節因子)を明らかにした。本研究では、この転写因子の遺伝子ノックアウトマウスの解析を進めて、味蕾が小さくなっていることを明らかにした。本年度は、発生過程の解析を進めて、味蕾原基の形成には異常は認められず、味蕾細胞のターンオーバーの変化によって味蕾の形態変化が生じていることを明らかにした。また、味覚行動解析によって、苦味に対する忌避に加えて、塩味に対する忌避性の感受性が大きく低下していることを明らかにした。甘味、酸味に対する感受性の低下も確認した。また、舌先端、舌基部、軟口蓋の3つの部位の味蕾について比較解析を進めて、軟口蓋の味蕾に最も大きな変化が生じていることを明らかにした。

  • 味蕾細胞分化モデルの実証/増殖能を失った前駆細胞を増殖させる培養系の確立

    2014.4 - 2017.3

    科学研究費補助金  基盤研究(C)

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    味蕾細胞分化モデルの実証/増殖能を失った前駆細胞を増殖させる培養系の確立

  • Mechanisms of taste bud cell differentiation/ Establishment of cell culture system from postmitotic taste bud precursor cells

    Grant number:26462818  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Hirohito Miura, KUSAKABE YUKO

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    Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

    Taste bud cells are derived from bipotential progenitor cells that give rise to both taste bud cells and surrounding keratinocytes. Sox2 is reported to be expressed intensely in taste bud primordia and is proposed to regulate the differentiation of bipotential progenitor cells into taste bud cells. However, detailed expression of Sox2 remained unclear. In this study, Sox2 expression was examined together with Prox1, which marks taste bud primordia, during taste bud development in the fungiform (FF), circumvallate (CV) and soft palate (SP) areas. The intense Sox2 was restricted to taste bud primordia at the emergence of taste bud primordia only in the FF forming area but not in the other areas. The intense Sox2 was restricted to taste bud cells and surrounding cells in all areas examined as development progressed. The expression patterns support that Sox2 does not serve as a cell fate selector between taste bud cells and surrounding keratinocytes but rather contribute to them both.

  • 基底細胞から味蕾へ:コンディショナル遺伝子改変によるターンオーバーの解明

    2011.4 - 2014.3

    科学研究費補助金  基盤研究(C)

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    基底細胞から味蕾へ:コンディショナル遺伝子改変によるターンオーバーの解明

  • Cell differentiation from basal cell to taste bud: Conditional recombination approach

    Grant number:23592742  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MIURA Hirohito, HARADA Shuitsu, NAKAYAMA Ayumi, OOKI Makoto

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    Grant amount:\5460000 ( Direct Cost: \4200000 、 Indirect Cost:\1260000 )

    Taste buds, the peripheral sensory organ for gustation, are comprised of 30-100 elongate cells and a small number of ovoid cells situated in the basal region. The basal cells in taste buds selectively express Shh, an evolutionally conserved signaling molecule. In this study, the Shh-expressing basal cells were genetically labeled with the expression of LacZ or EGFP marker protein by inducible gene recombination. Analysis of marker expression revealed (1) Shh-expressing cells are postmitotic, (2) Shh-expressing basal cells give rise to all three functional cell types in taste buds, type I (glia-like), type II (sweet, umami and bitter sensing ) and type III (sour sensing) cells.

  • 味蕾細胞分化メカニズムの解明:味細胞と味神経の相互作用を再現する培養系の利用

    2008.4 - 2011.3

    科学研究費補助金  基盤研究(C)

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    味蕾細胞分化メカニズムの解明:味細胞と味神経の相互作用を再現する培養系の利用

  • Mechanisms of taste cell differentiation : interaction between taste cells and taste nerves in reconstruction system

    Grant number:20592177  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    HARADA Shuitsu, NAKAYAMA Ayumi, MIURA Hirohito

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    The involvement of taste nerves in the regional differences in taste cell differentiation, and the regional differences in taste transduction machinery were analyzed between the soft palate and fungiform papillae. Our results suggested that taste nerves may not be involved in the regional differences in taste cell differentiation, and that the difference in bitter taste transduction may be caused by the differences in not only Gα proteins but also bitter taste receptors.

  • 哺乳類軟口蓋味蕾機能の重要性と種特異性

    2006.4 - 2008.3

    文部科学省  基板研究(C) 

    原田 秀逸, 三浦裕仁, 中山歩, 友成博

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  • 呈味増強物質探索システム“AGSS”の開発と塩分摂取低減のための新規物質探索

    2004.4 - 2005.3

    農林水産省農林水産技術会議  アグリバイオ実用化・産業化研究 

    日野 明寛, 日下 部裕子, 進藤洋一郎, 大倉哲也, 結城敏文, 三浦裕仁

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  • Molecular mechanisms of formation and reformation of taste system for reception and information transmission.

    Grant number:15209061  2003 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    NINOMIYA Yuzo, SHIGEMURA Noriatsu, Miura Hirohito

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    Grant amount:\36010000 ( Direct Cost: \27700000 、 Indirect Cost:\8310000 )

    The purpose of this study is to investigate molecular basis for taste reception and information transmission. To do this, by developing new approaches and improving methods, we examined 1) molecular expression of taste cells whose responses to various taste stimuli were recorded by loose-patch recording configuration, 2) similarities and differences of response profiles of taste cells generating action potentials with taste nerve fibers, and compared 3) responses in wild type and T1r3-KO and TRPM5-KO mice. The results demonstrated that taste cells responding to sweet substances did not always express sweet-response-related molecules, such as T1R3(receptor) and Ggust (G-protein). There was only less than 5% of taste cells responding to sweet substances that expressed synapse-related molecule, SNAP25. More than' 60% of taste cells generating action potentials responded to only one of the four basic taste stimuli and groupings of taste cells based on their best-stimulus and cluster analysis were similar to those of taste nerve fibers. T1R3-KO and TRPM5-KO mice showed residual responses to sweet substances. TRPM5-KO mice lacked temperature sensitivities in response to sweet substances. These results suggest that there probably exist multiple receptor and transduction systems for sweet taste. Taste cells generating action potentials in response to taste stimuli lacked conventional synapses. However, similarity of response profiles between taste cells with action potentials and taste nerve fibers to four basic taste stimuli indicates that taste information from taste cells generating action potentials transmitted to the nerve without major modification. Thereby, they may transmit taste information to the nerve by the pathway other than conventional synapses. TRPM5 channels act as not only channels responsible for transduction for sweet taste but also temperature sensor which produce temperature enhancement of sweet responses.

  • Molecular basis of gustatory system

    1999.4 - 2004.3

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Teaching Experience

  • 生理学

 

Social Activities

  • 味を感じるしくみ

    Role(s): Lecturer

    熊本県高等学校教育研究会  平成30年度 熊本県 高等学校教育研究会 家庭科研修  2018.11

  • 熊本県 高等学校教育研究会 家庭部会 研修会 講師

    Role(s): Lecturer

    2018.11

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    Audience: Teachers

  • 味を感じるしくみ

    Role(s): Lecturer

    鹿児島県高等学校教育研究会  平成30年度 鹿児島県 高等学校家庭科実践研究会  2018.8

  • 鹿児島県 高等学校教育研究会 家庭部会 研修会 講師

    Role(s): Lecturer

    2018.8

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    Audience: Teachers

  • 味覚を科学する

    Role(s): Lecturer

    鹿児島県学校栄養士協議会  平成30年度 鹿児島県 学校栄養士協議会 講演会  2018.5

  • 鹿児島県 栄養士協議会 講演会 講師

    Role(s): Lecturer

    2018.5

  • 味を感じる仕組み

    Role(s): Lecturer

    鹿児島市健康福祉局保健予防課  平成29年度 第2回食育教室  2017.10

  • 鹿児島市 市民講座 食育教室 講師

    Role(s): Lecturer

    2017.10

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    Audience: General

  • 味を感じる仕組み

    Role(s): Lecturer

    鹿児島市健康福祉局保健予防課  鹿児島市栄養業務担当者研修会  2017.8

  • 鹿児島市 栄養関係業務担当者研究会 講師

    Role(s): Lecturer

    2017.8

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    Audience: Teachers

    Type:Lecture

  • 食育と味覚の基礎:「味を感じるしくみ」

    Role(s): Lecturer

    鹿児島大学 教員免許状更新講習  2015 - 2021

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