研究者詳細 - 山本　雅史

論文 - 山本　雅史

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Kotoda N., Yamamoto M., Iwasaki M. . Citrus industry in Japan . Acta Horticulturae1448 751 - 763 2026年2月

記述言語：日本語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：Acta Horticulturae

Citrus fruit production in Japan currently stands at approximately 1.022 million tons, making it the number one fruit species cultivated domestically. Shiikuwasha (Citrus depressa Hayata) and Tachibana [C. tachibana (Makino) Tanaka] have grown wild in Japan from ancient times, so the citrus fruits currently on the market have either been introduced from overseas or developed through natural or artificial hybridization among the introduced species/cultivars and the two wild species. In Japan, after the Meiji Restoration (1868), cultivation of Satsuma mandarin began nationwide. From that time onwards, sweet orange, ponkan, and lemon were also introduced from overseas. Satsuma mandarin production increased from the 1950s, but gradually declined in the late 1970s. A breeding program was launched by a national research institute in 1937, and the first hybrid cultivar, ‘Kiyomi’, was registered in 1979, which led to the development of many excellent cultivars derived from ‘Kiyomi’, including ‘Shiranuhi’, ‘Harumi’, ‘Setoka’, and ‘Beni Madonna’. Meanwhile, research on citrus cultivation, storage, and sorting systems has progressed steadily. Japan has developed unique cultivation techniques such as mulch cultivation systems and plastic house cultivation for producing high-quality citrus. Regarding storage techniques, development is also underway on low-temperature storage with cool air and high humidity, and decay prevention techniques using ozone and UV irradiation. In addition to the conventional near-infrared method of determining sugar content and acidity, non-destructive sorting systems also use artificial intelligence (AI) technology, which has still room for further development over the next ten years. In the future, the citrus industry will also be faced with the urgent task of achieving labor-saving, low-cost cultivation, stable production, and adding high value to the products, as well as securing successors with a view to corporate management. We describe here cultural environment, cultivars and rootstocks, breeding program, cultural practice, pest and diseases, and marketing system of citrus fruit to overview the current status of citrus industry in Japan for better future.

DOI： 10.17660/ActaHortic.2026.1448.92

Scopus

勘米良祥多・石畑清武・林　真人・原田和彦・遠城道雄・赤木　功・山本雅史 . アセロラ(Malpighia glabra L.)およびMalpighia属植物におけるネコブセンチュウ抵抗性系統の探索ならびに台木特性 . 熱帯農業研究18 ( 2 ) 63 - 72 2025年12月査読

Yamamoto Masashi, Maeno Kinya, Shinohara Kazutaka, Higashi Akihiro, Takada Norio, Sakaue Takami, Shimada Atsushi, Kozai Naoko, Kozaki Itaru . Long-term Preservation of Japanese Pear (Pyrus pyrifolia (Burm. f.) Nakai) Pollen . The Horticulture Journal94 ( 4 ) 438 - 443 2025年10月査読

担当区分：筆頭著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：The Japanese Society for Horticultural Science

The long-term preservation of pollen is imperative for the conservation of genetic resources and cross-breeding. In the present study, we demonstrated that preserved pollen of the Japanese pear (Pyrus pyrifolia (Burm. f.) Nakai) retains viability for a minimum of 50 years. In 1973, fresh pollen from ‘Chojuro’ was collected and immediately subjected to freeze-drying for 15 minutes at pressures ranging from 3.3 to 13 Pa. The pollen was then sealed in ampules under a vacuum and preserved at −20°C for 40 years followed by −30°C for 10 years. In 2013 and 2023, the viability of this pollen was investigated. The rate of pollen germination showed no significant differences between the preserved and fresh pollen. In 2013, flowers of ‘Kosui’ and ‘Niitaka’ were pollinated with pollen preserved for 40 years as well as fresh pollen. The growth of the pollen tubes in the pistils of preserved pollen was normal. Pollination with preserved pollen resulted in 77.1 and 86.7% fruit set, yielding 3.2 and 8.3 perfect seeds per fruit in ‘Kosui’ and ‘Niitaka’, respectively. In 2023, the fruit set and perfect seeds per fruit of ‘Kosui’ pollinated with 50-year-preserved pollen were 66.7% and 3.5, respectively. There were no significant differences between preserved and fresh either year for either trait. The results of the present study demonstrate that the viability of pear pollen can be sustained for a minimum of 50 years when preserved in a freeze-dried state at temperatures ranging from −20 to −30°C.

DOI： 10.2503/hortj.szd-064

CiNii Research

YAMAMOTO Masashi . Development of chromosome observation methods in acerola (Malpighia glabra L.) . Japan Agricultural Research Quarterly: JARQ59 ( 3 ) 227 - 231 2025年7月査読

担当区分：筆頭著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Japan International Research Center for Agricultural Sciences

A chromosome preparation method using young leaves of acerola (Malpighia glabra) was developed. The young leaves were cut into approximately 2-mm2 for enzymatic maceration and air-drying (EMA). For EMA, an enzyme mixture containing 2% Cellulase Onozuka RS, 1.5% Macerozyme R200 (Yakult), 0.3% Pectolyase Y-23 (Seishin Pharmaceutical Co., Ltd.), and 1 mM EDTA, pH 4.2, at 37°C for 30 min was optimal for chromosome preparation since good preparations, with all 20 chromosomes relatively extended and well-spread without cytoplasm, were observed. There were 11.2 preparations in large leaves (8 to 10 mm long and 3 to 4 mm wide) and 4.2 preparations in small leaves (6 to 8 mm long and 1 to 2 mm wide). Chromomycin A3 (CMA)-positive (+) bands were noted in the telomeric positions of eight chromosomes. 4′-6-diamidino-2-phenylindole (DAPI)-negative bands (−) corresponded to CMA+ bands. The numbers and positions of CMA+ bands were the same in the two cultivars examined: ‘Florida Sweet’ and ‘Sanmi-kei (Hosoba).’ The methods developed in the present study are promising for further cytogenetic studies in acerola.

DOI： 10.6090/jarq.24j12

香西直子・實石汰一・山本雅史・福留弘康・廣瀬潤・西澤優・兒玉真紀 . 花粉親が在来アーモンドの結実性および果実形質に及ぼす影響 . 鹿児島大学農場研報46 13 - 18 2025年3月査読

西澤　優・香西直子・宇都量子・福留弘康・廣瀬　潤・兒玉真紀・山本雅史・朴　炳宰・遠城道雄 . 隔年交互結実がビワ（Eriobotrya japonica (Thunb.) Lindl.）の果実重，結果枝の形状および個葉の光合成能力と葉の炭水化物蓄積に及ぼす影響 . 園芸学研究24 ( 1 ) 45 - 52 2025年1月査読

Witharana EP, Iwasaki T, San MH, Jayawardana NU, Kotoda N, Yamamoto M, Nagano Y . Subfamily evolution analysis using nuclear and chloroplast data from the same reads. . Scientific Reports15 ( 1 ) 687 2025年1月査読国際共著

Shirasawa, K., Harada, K., Haramoto, N., Aoki, H., Kammera, S., Yamamoto, M. and Nishizawa, Y. . Chromosome-scale genome assembly of acerola (Malpighia emarginata DC.) . DNA Research31 ( 6 ) dsae029 - dsae029 2024年12月査読

東　弘奈・一谷勝之・山本雅史・志水勝好 . ボタンボウフウ（Peucedanum japonicum Thumb.）の鹿児島県本土および島嶼部における分布に関する研究 . 熱帯農業研究17 ( 2 ) 45 - 50 2024年12月査読

Yamamoto, M., Kusuki, S., Furusawa, N., Ikeda, S., Fujino, Y., Kozai, N. . Tissue culture of sweet type acerola (Malpighia glabra L.) ‘Florida Sweet’ . Tropical Agriculture and Development68 ( 4 ) 82 - 85 2024年12月査読

Oe, M., Asikin, Y., Kashima, M., Yamamoto, M., Mitsube, F., Lin, S.-Y., Takara, Wada, K. . Genetic characteristics and volatile organic compounds of leaves and fruit edible parts of Citrus depressa Hayata from different geographic origins . Horticulturae10 939 - 939 2024年9月査読国際共著

山本雅史・桑原佳大・窪田瑛水・島田温史・吉﨑由美子・渡部由香・朴　炳宰・香西直子 . 鹿児島県大隅半島南部の特産カンキツである辺塚ダイダイの類縁関係と果実特性 . 熱帯農業研究17 ( 1 ) 1 - 11 2024年6月査読

Yamamoto, M., Arita. S., Kozai, N. . Simple methods for producing tetraploids in polyembryonic citrus . Japan Agricultural Research Quarterly58 ( 2 ) 107 - 113 2024年4月査読

香西直子・井山尚紀・濱田康平・山本雅史・福留弘康・廣瀬　潤・西澤　優 . 在来アーモンドの鹿児島県における休眠および開花特性 . 鹿児島大学農場研報45 1 - 6 2024年3月査読

Asikin, Y., Tamura, Y., Aono, Y., Kusano, M., Shiba, H., Yamamoto, M., Mitsube, F., Lin, S., Takara, K., Wada, K. . Multivariate profiling of metabolites and volatile organic compounds in Citrus depressa Hayata fruits from Kagoshima, Okinawa, and Taiwan . Foods ( MDPI )12 ( 15 ) 2951 - 2951 2023年8月査読国際共著

Yang, X., Kitajima, A., Nagano, Y., Yamamoto, M. . Citrus genetic resources grown in Yunnan Province, China . Tropical Agriculture and Development67 ( 2 ) 47 - 53 2023年6月査読国際共著

Yamamoto, M. . Self and cross-incompatibility of Citrus depressa Hayata (Shiikuwasha) and its relatives . The Horticulture Journal92 ( 2 ) 134 - 141 2023年4月査読

山本雅史・谷　佳那美・香西直子 . 鹿児島県黒島の在来カンキツ . 南太平洋研究43 ( 1/2 ) 1 - 10 2023年3月査読

山本雅史・伊東孝峻・川口昭二・野村哲也・福留弘康・勘米良祥多 . 多胚性カンキツの胚数およびその雑種実生出現 . 鹿児島大学農学部附属農場研究報告44 1 - 4 2023年3月査読

島田温史・香西直子・山本雅史 . 種々の方法によるアボカドの耐寒性評価 . 熱帯農業研究15 ( 2 ) 95 - 100 2022年12月査読

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