研究者詳細 - 橋本　文雄

論文 - 橋本　文雄

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橋本文雄 . 花と健康 . 口腔衛生学会雑誌73 ( 2 ) 90 - 96 2023年

Wang Zitai, Okutsu Kayu, Yoshizaki Yumiko, Futagami Taiki, Tamaki Hisanori, Maruyama Takuro, Toume Kazufumi, Komatsu Katsuko, Hashimoto Fumio, Takamine Kazunori . Massa Medicata Fermentataの酵素活性と揮発性に対する微生物発酵の影響(Effects of microbial fermentation on enzyme activity and volatile properties of Massa Medicata Fermentata) . Traditional & Kampo Medicine9 ( 1 ) 10 - 17 2022年4月

記述言語：英語出版者・発行元：John Wiley & Sons Australia, Ltd

Massa Medicata Fermentata(MMF)はAspergillus属やRhizopus属による微生物発酵でコムギと数種のハーブから調製されるので、微生物発酵の影響について検討した。National Standard for Chinese Patent Drugsに従ってMMFの原材料を混合し、Aspergillus oryzae培養で調製(MMF-A)またはRhizopus oryzae培養で調製(MMF-R)した。MMFの調製により酵素活性は上昇した。MMF-AはMMF-Rよりもα-アミラーゼとリパーゼの活性が高かった。プロテアーゼ活性はMMF-Rがわずかに高かった。テルペノイドは発酵で低下したが、2,3-ブタンジオール、アセトイン、グアヤコールはMMFだけで検出された。1-オクテン-3-オールのC8化合物はMMF-RよりもMMF-Aで高く、4-ビニルグアヤコールとピラジンの芳香族化合物はMMF-Rで高かった。

Wang Z., Okutsu K., Yoshizaki Y., Futagami T., Tamaki H., Maruyama T., Toume K., Komatsu K., Hashimoto F., Takamine K. . Effects of microbial fermentation on enzyme activity and volatile properties of Massa Medicata Fermentata . Traditional and Kampo Medicine9 ( 1 ) 10 - 17 2022年4月

記述言語：日本語出版者・発行元：Traditional and Kampo Medicine

Aim: Massa Medicata Fementata (MMF) is a crude drug used in East Asia to treat anorexia and dyspepsia. It is prepared from wheat and several herbs through microbial fermentation using Aspergillus sp. and Rhizopus sp. There is great difference in the quality of commercial MMF, and the microbes of MMF are suggested to affect its quality. We investigated the effects of microbial fermentation on the quality of MMF. Methods: Raw materials of MMF were mixed according to the ratio listed in the National Standard for Chinese Patent Drugs, and MMF was prepared using pure cultures of Aspergillus oryzae or Rhizopus oryzae. Digestive enzyme activities (α-amylase, protease, and lipase) and volatile compounds were measured using an analytical kit and GC–MS, respectively. Results: Enzyme activity increased in MMF. MMF prepared with A. oryzae (MMF-A) showed higher α-amylase and lipase activities than that prepared with R. oryzae (MMF-R). Protease activity was marginally higher in MMF-R than in MMF-A. GC–MS analysis revealed that terpenoids decreased with fermentation; however, 2,3-butanediol, acetoin, and guaiacol were detected in MMF only. C8 compounds such as 1-octen-3-ol were higher in MMF-A than MMF-R; however, aromatic compounds such as 4-vinylguaiacol and pyrazines were higher in MMF-R than MMF-A. Conclusion: Microbial fermentation contributes to increased enzyme activity and changes in MMF volatiles. These properties of MMF were considerably affected by the microbes used, and it is proposed in this study that it is important to have microbial control in the production of commercial MMF.

DOI： 10.1002/tkm2.1303

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Takatori Y., Shimizu K., Yauwapaksopon D., Nakamura Y., Oshima H., Hashimoto F. . Flavonoid 3',5'-Hydroxylase (F3'5'H) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] . The Horticulture Journal91 ( 2 ) 229 - 239 2022年

記述言語：英語出版者・発行元：一般社団法人　園芸学会

This study investigated genetic polymorphism related to anthocyanin composition variation in lisianthus (Eustoma grandiflorum) flower petals. Three different bands were detected by genomic polymerase chain reaction using specific primers based on the reported flavonoid 3',5'-hydroxylase (F3'5'H) gene sequence. Our genetic study revealed that they were allelic. They were named F3'5'H1-1, F3'5'H1-2, and f3'5'h1-2. F3'5'H1-1 and F3'5'H1-2 differed in the length of the intron. Allele f3'5'h1-2 possessed a retrotransposon insertion in the first exon, but otherwise, its gene sequence was almost identical to that of F3'5'H1-2. This retrotransposon is a Ty1-copia type retrotransposon and was designated as the retrotransposon of Eustoma grandiflorum 1 (rTeg1). The mauve flower line ‘ME’ and red-purple flower line ‘PRP’ were homozygous for F3'5'H1-1 and mostly accumulated cyanidin, with a small amount of delphinidin, in flower petals (the Cy major phenotype). The violet flower line ‘RV’ was homozygous for F3'5'H1-2 and accumulated only delphinidin (the Dp major phenotype). The pink-red flower line ‘AK’ was homozygous for f3'5'h1-2, accumulated pelargonidin, and lacked delphinidin (the Pg major-non Dp phenotype). Segregation analysis of the F2 population from ‘ME’ × ‘RV’ revealed that F3'5'H1-2 was associated with the Dp major phenotype, and the delphinidin major trait was dominant in the Cy major phenotype. All the Cy major phenotype plants in the F2 population possessed homozygous F3'5'H1-1. Genetic analysis of the F2 population from the cross between delphinidin-containing and delphinidin-lacking strains revealed that the homozygous f3'5'h1-2 genotype co-segregated with the Pg major-non Dp phenotype, which was recessive to the delphinidin-containing phenotypes. Expression analysis of the F3'5'H gene demonstrated that an abnormal mRNA was transcribed from the F3'5'H gene in the homozygous f3'5'h1-2 line by the insertion of rTeg1. Therefore, these F3'5'H gene polymorphisms can be used as DNA markers for breeding lisianthus based on flower color.

DOI： 10.2503/hortj.utd-320

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Zitai Wang, Kayu Okutsu*, Taiki Futagami, Yumiko Yoshizaki, Hisanori Tamaki, Takuro Maruyama, Kazufumi Toume, Katsuko Komatsu, Fumio Hashimoto and Kazunori Takamine. . Microbial Community Structure and Chemical Constituents in Shinkiku, a Fermented Crude Drug Used in Kampo Medicine. . Frontiers in Nutrition115 ( 7 ) 2020年7月査読

濵田延枝, 田浦一成, 野村哲也, 中野八伯, 橋本文雄, 清水圭一, 朴炳宰, 遠城道雄 . トルコギキョウの鹿児島大学オリジナル品種作出をめざした育成系統の生育および切り花品質調査 . 鹿児島大学農場研究報告 2020年3月査読

Md. Abdur Rauf Sarkar, Wakana Otsu, Akihiro Suzuki, Fumio Hashimoto, Toyoaki Anai and Satoshi Watanabe*. . Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed. . Breeding Science70 265 - 276 2020年3月査読

Atsushi SHIMADA, Kaoru KURAMOTO, Byoung-Jae PARK, Fumio HASHIMOTO, and Masashi YAMAMOTO*. . Relationship between Temperature and Fruit Quality during Ripening Period in Passion Fruit. . Trop. Agr. Develop.64 ( 2 ) 47 - 53 2020年2月査読

Sarkar Md. Abdur Rauf, Otsu Wakana, Suzuki Akihiro, Hashimoto Fumio, Anai Toyoaki, Watanabe Satoshi . Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed . Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ70 ( 3 ) 265 - 276 2020年

出版者・発行元：日本育種学会

Novel mutant alleles related to isoflavone content are useful for breeding programs to improve the disease resistance and nutritional content of soybean. However, identification of mutant alleles from high-density mutant libraries is expensive and time-consuming because soybean has a large, complicated genome. Here, we identified the gene responsible for increased genistein-to-daidzein ratio in seed of the mutant line F333ES017D9. For this purpose, we used a time- and cost-effective approach based on selective genotyping of a small number of F2 plants showing the mutant phenotype with nearest-neighboring-nucleotide substitution–high-resolution melting analysis markers, followed by alignment of short reads obtained by next-generation sequencing analysis with the identified locus. In the mutant line, GmCHR5 harbored a single-base deletion that caused a change in the substrate flow in the isoflavone biosynthetic pathway towards genistein. Mutated GmCHR5 was expressed at a lower level during seed development than wild-type GmCHR5. Ectopic overexpression of GmCHR5 increased the production of daidzein derivatives in both the wild-type and mutant plants. The present strategy will be useful for accelerating identification of mutant alleles responsible for traits of interest in agronomically important crops.

DOI： 10.1270/jsbbs.19134

Scopus

PubMed

SHIMADA Atsushi, KURAMOTO Kaoru, PARK Byoung-Jae, HASHIMOTO Fumio, YAMAMOTO Masashi . Relationship between Temperature and Fruit Quality during Ripening Period in Passion Fruit . Tropical Agriculture and Development64 ( 2 ) 47 - 53 2020年

出版者・発行元：日本熱帯農業学会

The optimum temperature during fruit ripening period of passion fruit (Passiflora edulis) was elucidated in the present study. 'Summer Queen' (P. edulis × P. edulis f. flavicarpa) and 'Ruby Star' (P. edulis × P. edulis f. flavicarpa) showed a greenish fruit peel and low total soluble solids/titratable acidity (TSS/TA) ratio at 15°C. In 'Summer Queen', a high °Brix, low titratable acidity, and high TSS/TA ratio were observed at 25°C. In 'Ruby Star', the peel coloration was highly favorable at 25°C. In both cultivars, the peel tended to be yellowish and °Brix of the juice was low at 35°C. These results indicate that the optimal temperature for passion fruit ripening was 25°C. In addition, we elucidated the varietal differences in the ripening characteristics of fruit on and off the vine.

DOI： 10.11248/jsta.64.47

Wang Z, Okutsu K, Futagami T, Yoshizaki Y, Tamaki H, Maruyama T, Toume K, Komatsu K, Hashimoto F, Takamine K . Microbial Community Structure and Chemical Constituents in Shinkiku, a Fermented Crude Drug Used in Kampo Medicine. . Frontiers in nutrition7 115 2020年

Md. Abdur Rauf Sarkar, Satoshi Watanabe, Akihiro Suzuki, Fumio Hashimoto, Toyoaki Anai. . Identification of novel MYB transcription factors involved in the isoflavone biosynthetic pathway by using the combination screening system with agroinfiltration and hairy root transformation. . Plant Biotechnology36 241 - 251 2019年3月査読

Sarkar Md. Abdur Rauf, Watanabe Satoshi, Suzuki Akihiro, Hashimoto Fumio, Anai Toyoaki . Identification of novel MYB transcription factors involved in the isoflavone biosynthetic pathway by using the combination screening system with agroinfiltration and hairy root transformation . Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ36 ( 4 ) 241 - 251 2019年

出版者・発行元：日本植物細胞分子生物学会

Soybean isoflavones are functionally important secondary metabolites that are mainly accumulated in seeds. Their biosynthetic processes are regulated coordinately at the transcriptional level; however, screening systems for key transcription factors (TFs) are limited. Here we developed a combination screening system comprising a simple agroinfiltration assay and a robust hairy root transformation assay. First, we screened for candidate MYB TFs that could activate the promoters of the chalcone synthase (CHS) gene GmCHS8 and the isoflavone synthase (IFS) genes GmIFS1 and GmIFS2 in the isoflavone biosynthetic pathway. In the agroinfiltration assay, we co-transformed a LjUbi (Lotus japonicus polyubiquitin gene) promoter-fused MYB gene with target promoter-fused GUS (β-glucuronidase) gene constructs, and identified three genes (GmMYB102, GmMYB280, and GmMYB502) as candidate regulators of isoflavone biosynthesis. We then evaluated the functional regulatory role of identified three MYB genes in isoflavone biosynthesis using hairy roots transformation assay in soybean for the accumulation of isoflavones. Three candidate MYB genes showed an increased accumulation of total isoflavones in hairy root transgenic lines. Accumulation of total isoflavones in the three MYB-overexpressing lines was approximately 2-to 4-folds more than that in the vector control, confirming their possible role to regulate isoflavone biosynthesis. However, the significant accumulation of authentic GmCHS8, GmIFS1, and GmIFS2 transcripts could not be observed except for the GmMYB502-overexpressing line. Therefore, the analysis of isoflavone accumulation in transgenic hairy root was effective for evaluation of transactivation activity of MYB TFs for isoflavone biosynthetic genes. Our results demonstrate a simple and robust system that can potentially identify the function of orphan TFs in diverse plant metabolic pathways.

DOI： 10.5511/plantbiotechnology.19.1025a

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PubMed

Manikharda, Makoto Takahashi, Mika Arakaki, Kaoru Yonamine, Fumio Hashimoto, Kensaku Takara, Koji Wada. . Influence of Fruit Ripening on Color, Organic Acid Contents, Capsaicinoids, Aroma Compounds, and Antioxidant Capacity of Shimatogarashi (Capsicum frutescens). . J Oleo Science67 ( 1 ) 113 - 123 2018年2月査読

Manikharda, Takahashi Makoto, Arakaki Mika, Yonamine Kaoru, Hashimoto Fumio, Takara Kensaku, Wada Koji . Influence of Fruit Ripening on Color, Organic Acid Contents, Capsaicinoids, Aroma Compounds, and Antioxidant Capacity of Shimatogarashi (Capsicum frutescens) . 日本油化学会誌67 ( 1 ) 113 - 123 2018年

出版者・発行元：公益社団法人日本油化学会

Shimatogarashi (Capsicum frutescens) is a typical chili pepper domesticated in southern Japan. Important traits of Shimatogarashi peppers, such as color; proportion of organic acids, capsaicinoids, and aromatic compounds; and antioxidant activity in three stages of maturity (green (immature), orange (turning), and red (mature) stages) were characterized. The results indicated that the concentration of organic acids, including ascorbic, citric, and malic acid, increased during ripening. In addition, the amount of capsaicinoids, which are responsible for the pungent taste of chili peppers, increased as the fruit matured to the orange and red stages. The volatile compound profile of Shimatogarashi was dominated by the presence of esters, which mainly contributed to fruity notes. The total amount of volatile compounds analyzed by gas chromatography-headspace solid-phase microextraction (GC-HS-SPME), especially esters, decreased as the fruit changed in color from green to red. This was in contrast to the amount of terpenoids, especially limonene, which increased at the red stage, denoting a change in flavor from fruity to a more citrus-like aroma. Based on the total phenolic content (TPC), the oxygen radical absorbance capacity (ORAC) and the diphenylpicrylhydrazyl (DPPH) free radical method, the antioxidant capacity of Shimatogarashi showed an increase at the mature red stage. However, while the red stage showed higher pungency and antioxidant capacity as well as an attractive color, the results of aromatic compound analysis revealed that the immature green stage had the advantages of having pleasant fruity smell, making it suitable for use in condiments.

DOI： 10.5650/jos.ess17156

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PubMed

Su Xiaoqin, Zhang Gao-Ju, Ma Yan, Chen Mao, Chen Sheng-Hu, Duan Shuang-Mei, Wan Jin-Qiong, Fumio Hashimoto, Lin Zhi, Li Jia-Hua, Zhao Ming. . Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh tea and In Vitro Antioxidant Activity. . Applied Sciences6 161 2016年4月査読

髙取由佳, 櫟本裕太郎, 清水圭一, 橋本文雄 . 野生種トルコギキョウを起源とする二重咲き花形の遺伝様式 . 園芸学研究14 ( 4 ) 341 - 348 2015年10月査読

Kayu Okutsu, Yumiko Yoshizaki, Natsumi Ikeda, Tatsuro Kusano, Fumio Hashimoto, Kazunori Takamine . Antioxidants in Heat-Processed Koji and Evaluation of Antioxidant Production Mechanisms. . Food Chem.187 364 - 369 2015年5月査読

Yuka Takatori, Keiichi Shimizu, Jun Ogata, Hiroki Endo, Kanji Ishimaru, Shigehisa Okamoto, Fumio Hashimoto . Cloning of the Flavonoid 3'-Hydroxylase Gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3'H) and Complementation of an F3'H-Deficient Mutant of Ipomoea nil (L.) Roth. by Heterologous Expression of EgF3'H. . The Horticulture J.82 ( 4 ) 131 - 139 2015年4月査読

Phyu Phyu Khine Zar, Satoshi Yano, Kozue Sakao, Fumio Hashimoto, Takayuki Nakano, Makoto Fujii, De-Xing Hou. . In vitro Anticancer Activity of Loquat Tea by Inducing Apoptosis in Human Leukemia Cells. . Biosci. Biotechnol. Biochem.78 ( 10 ) 1731 - 1737 2014年10月査読

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