Updated on 2023/11/15

写真a

 
Masahiro Shibata
 
Organization
Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Neurology Professor
Title
Professor

Degree

  • 博士（医学） ( 2001.3   大阪大学 )

Research Areas

  • Life Science / Anatomy

  • Others / Others  / 組織細胞化学

  • Others / Others  / 肉眼解剖学

Research History

  • Kagoshima University   Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course   Professor

    2014.9

Professional Memberships

  • 日本神経科学会

    1999.4

  • 日本解剖学会

    1998.6

 

Papers

  • Chigure Suzuki, Junji Yamaguchi, Takahito Sanada, Juan Alejandro Oliva Trejo, Souichirou Kakuta, Masahiro Shibata, Isei Tanida, Yasuo Uchiyama .  Lack of Cathepsin D in the central nervous system results in microglia and astrocyte activation and the accumulation of proteinopathy-related proteins. .  Scientific reports12 ( 1 ) 11662 - 11662   2022.7Invited Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    Neuronal ceroid lipofuscinosis is one of many neurodegenerative storage diseases characterized by excessive accumulation of lipofuscins. CLN10 disease, an early infantile neuronal ceroid lipofuscinosis, is associated with a gene that encodes cathepsin D (CtsD), one of the major lysosomal proteases. Whole body CtsD-knockout mice show neurodegenerative phenotypes with the accumulation of lipofuscins in the brain and also show defects in other tissues including intestinal necrosis. To clarify the precise role of CtsD in the central nervous system (CNS), we generated a CNS-specific CtsD-knockout mouse (CtsD-CKO). CtsD-CKO mice were born normally but developed seizures and their growth stunted at around postnatal day 23 ± 1. CtsD-CKO did not exhibit apparent intestinal symptoms as those observed in whole body knockout. Histologically, autofluorescent materials were detected in several areas of the CtsD-CKO mouse's brain, including: thalamus, cerebral cortex, hippocampus, and cerebellum. Expression of ubiquitin and autophagy-associated proteins was also increased, suggesting that the autophagy-lysosome system was impaired. Microglia and astrocytes were activated in the CtsD-CKO thalamus, and inducible nitric oxide synthase (iNOS), an inflammation marker, was increased in the microglia. Interestingly, deposits of proteinopathy-related proteins, phosphorylated α-synuclein, and Tau protein were also increased in the thalamus of CtsD-CKO infant mice. Considering these results, we propose thatt the CtsD-CKO mouse is a useful mouse model to investigate the contribution of cathepsin D to the early phases of neurodegenerative diseases in relation to lipofuscins, proteinopathy-related proteins and activation of microglia and astrocytes.

    DOI： 10.1038/s41598-022-15805-3

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  • Mari Isobe, Yumika Suzuki, Hideshi Sugiura, Masahiro Shibata, Yuki Ohsaki, Satoshi Kametaka .  Novel cell-based system to assay cell-cell fusion during myotube formation .  Biomedical Research (Japan)43 ( 4 ) 107 - 114   2022Invited Reviewed International journal

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    A live assay tool has been established to uncover the precise molecular mechanisms underlying complex cell fusion events in myoblasts. The novel cell-based assay, HiMy (HiBiT-based myo-blast fusion), utilizes a recently developed split-luciferase technology. The assay successfully detected cell fusion in differentiating C2C12 myoblast cultures. This allowed us to measure mixing of the cytoplasm, which occurred several hours after the initiation of C2C12 differentiation. Un-like what was reported earlier, the fusion was detected a few hours after the initiation of differen-tiation. Thus, this assay is sensitive enough to monitor fusion events before they become detectable using conventional methods. Furthermore, a panel of laboratory compounds, including a variety of inhibitors of cellular enzymes or activities, were assayed using the HiMy assay. Lovas-tatin, a cholesterol biogenesis inhibitor, decreased HiMy activity by approximately 50%. In con-trast, mevalonolactone, a precursor for cholesterol synthesis, increased fusion activity. These results confirmed the previous finding that the amount of cellular cholesterol positively correlates with the rate of myoblast fusion during myogenesis. These results indicate that the novel cell fusion assay is a quick, accurate, and robust method to monitor intercellular fusion events.

    DOI： 10.2220/biomedres.43.107

    Scopus

  • Koga D., Kusumi S., Shibata M., Watanabe T. .  Applications of Scanning Electron Microscopy Using Secondary and Backscattered Electron Signals in Neural Structure .  Frontiers in Neuroanatomy15   2021.12Invited Reviewed International journal

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    Language：Japanese   Publisher：Frontiers in Neuroanatomy  

    Scanning electron microscopy (SEM) has contributed to elucidating the ultrastructure of bio-specimens in three dimensions. SEM imagery detects several kinds of signals, of which secondary electrons (SEs) and backscattered electrons (BSEs) are the main electrons used in biological and biomedical research. SE and BSE signals provide a three-dimensional (3D) surface topography and information on the composition of specimens, respectively. Among the various sample preparation techniques for SE-mode SEM, the osmium maceration method is the only approach for examining the subcellular structure that does not require any reconstruction processes. The 3D ultrastructure of organelles, such as the Golgi apparatus, mitochondria, and endoplasmic reticulum has been uncovered using high-resolution SEM of osmium-macerated tissues. Recent instrumental advances in scanning electron microscopes have broadened the applications of SEM for examining bio-specimens and enabled imaging of resin-embedded tissue blocks and sections using BSE-mode SEM under low-accelerating voltages; such techniques are fundamental to the 3D-SEM methods that are now known as focused ion-beam SEM, serial block-face SEM, and array tomography (i.e., serial section SEM). This technical breakthrough has allowed us to establish an innovative BSE imaging technique called section-face imaging to acquire ultrathin information from resin-embedded tissue sections. In contrast, serial section SEM is a modern 3D imaging technique for creating 3D surface rendering models of cells and organelles from tomographic BSE images of consecutive ultrathin sections embedded in resin. In this article, we introduce our related SEM techniques that use SE and BSE signals, such as the osmium maceration method, semithin section SEM (section-face imaging of resin-embedded semithin sections), section-face imaging for correlative light and SEM, and serial section SEM, to summarize their applications to neural structure and discuss the future possibilities and directions for these methods.

    DOI： 10.3389/fnana.2021.759804

    Scopus

  • Masao Horie, Nozomu Yoshioka, Satoshi Kusumi, Hiromi Sano, Masayuki Kurose, Izumi Watanabe-Iida, Ibrahim Hossain, Satomi Chiken, Manabu Abe, Kensuke Yamamura, Kenji Sakimura, Atsushi Nambu, Masahiro Shibata, Hirohide Takebayashi .  Disruption of dystonin in Schwann cells results in late-onset neuropathy and sensory ataxia .  GLIA68 ( 11 ) 2330 - 2344   2020.11Invited Reviewed International journal

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    Publisher：Wiley  

    Dystonin (Dst) is a causative gene for Dystonia musculorum (dt) mice, which is an inherited disorder exhibiting dystonia-like movement and ataxia with sensory degeneration. Dst is expressed in a variety of tissues, including the central nervous system and the peripheral nervous system (PNS), muscles, and skin. However, the Dst-expressing cell type(s) for dt phenotypes have not been well characterized. To address the questions whether the disruption of Dst in Schwann cells induces movement disorders and how much impact does it have on dt phenotypes, we generated Dst conditional knockout (cKO) mice using P0-Cre transgenic mice and Dst gene trap mice. First, we assessed the P0-Cre transgene-dependent Cre recombination using tdTomato reporter mice and then confirmed the preferential tdTomato expression in Schwann cells. In the Dst cKO mice, Dst mRNA expression was significantly decreased in Schwann cells, but it was intact in most of the sensory neurons in the dorsal root ganglion. Next, we analyzed the phenotype of Dst cKO mice. They exhibited a normal motor phenotype during juvenile periods, and thereafter, started exhibiting an ataxia. Behavioral tests and electrophysiological analyses demonstrated impaired motor abilities and slowed motor nerve conduction velocity in Dst cKO mice, but these mice did not manifest dystonic movements. Electron microscopic observation of the PNS of Dst cKO mice revealed significant numbers of hypomyelinated axons and numerous infiltrating macrophages engulfing myelin debris. These results indicate that Dst is important for normal PNS myelin organization and Dst disruption in Schwann cells induces late-onset neuropathy and sensory ataxia. Main points: Dystonin (Dst) disruption in Schwann cells results in late-onset neuropathy and sensory ataxia. Dst in Schwann cells is important for normal myelin organization in the peripheral nervous system.

    DOI： 10.1002/glia.23843

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/glia.23843

  • Kusumi Satoshi, Koga Daisuke, Shibata Masahiro, Watanabe Tsuyoshi .  Section SEM and Serial Section SEM .  KENBIKYO55 ( 1 ) 18 - 22   2020

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    Publisher：The Japanese Society of Microscopy  

    <p>Section scanning electron microscopy (SEM) is a novel SEM technique based on observation of backscattered electron (BSE) images of tissue sections embedded in resin on glass microscope slides. Using this technique, high-resolution transmission electron microscopy (TEM)-like images can be obtained without the need to use specialized techniques such as ultramicrotomy. In this paper, we introduce three techniques based on the SEM method: 1) BSE imaging of semithin sections; 2) correlation of immunofluorescence and BSE images of the semithin sections; and 3) combination of a cryosectioning method (the Tokuyasu method) with section SEM. Additionally, we describe serial section SEM, which is a novel 3D imaging method, and discuss the possibilities for use of these SEM imaging techniques for tissue sections.</p>

    DOI： 10.11410/kenbikyo.55.1_18

  • Satoshi Kusumi, Daisuke Koga, Tsuyoshi Watanabe, Masahiro Shibata .  Combination of a cryosectioning method and section scanning electron microscopy for immuno-scanning electron microscopy .  Biomedical Research (Japan)39 ( 1 ) 21 - 25   2018Invited Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Biomedical Research Foundation  

    We describe a novel immuno-scanning electron microscopy (SEM) technique that combines both Tokuyasu’s cryosectioning and section SEM methods. In this technique, semithin cryosections, cut according to the Tokuyasu method, were adhered to glass microscope slides, immunostained for bio-molecules of interest and observed by confocal laser scanning microscopy. The same sections were subsequently embedded in epoxy resin and ultrathin sections were cut on an ultramicrotome. These were then observed by SEM using a backscattered electron detector. Correlation between immunofluorescence and SEM images was performed in the same area of the cryosection. Immuno-SEM was also performed using a FluoroNanogold-labeled secondary antibody. This novel immuno-SEM method can provide ultrastructural information of cell organelles in relation to associated molecules, such as Golgi- and ER-associated proteins. This novel immuno-SEM technique has the potential to be widely used.

    DOI： 10.2220/biomedres.39.21

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  • Kentaro Takakura, Shogo Ito, Junya Sonoda, Koji Tabata, Motoko Shiozaki, Kaoru Nagai, Masahiro Shibata, Masato Koike, Yasuo Uchiyama, Takahiro Gotow .  Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functions .  Heliyon3 ( 11 ) e00462 - e00462   2017.11Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functionsInvited Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Ltd  

    The genus Cordyceps and its specific ingredient, cordycepin, have attracted much attention for multiple health benefits and expectations for lifespan extension. We analyzed whether Cordyceps militaris (CM), which contains large amounts of cordycepin, can extend the survival of Dahl salt-sensitive rats, whose survival was reduced to ∼3 months via a high-salt diet. The survival of these life-shortened rats was extended significantly when supplemented with CM, possibly due to a minimization of the effects of stroke. Next, we analyzed the effect of CM on hypertension-sensitive organs, the central nervous systems (CNS), heart, kidney and liver of these rats. We attempted to ascertain how the organs were improved by CM, and we paid particular attention to mitochondria and autophagy functions. The following results were from CM-treated rats in comparison with control rats. Microscopically, CNS neurons, cardiomyocytes, glomerular podocytes, renal epithelial cells, and hepatocytes all were improved. However, immunoblot and immunohistochemical analysis showed that the expressions of mitochondria-related proteins, ATP synthase β subunit, SIRT3 and SOD2, and autophagy-related proteins, LC3-II/LC3-I ratio and cathepsin D all were reduced significantly in the CNS neurons, but increased significantly in the cells of the other three organs, although p62 was decreased in its expression in all the organs tested. Activity of Akt and mTOR was enhanced but that of AMPK was reduced in the CNS, while such kinase activity was completely the opposite in the other organs. Together, the influence of CM may differ between mitochondria and autophagy functioned between the two organ groups, as mitochondria and autophagy seemed to be repressed and promoted, respectively, in the CNS, while both mitochondria and autophagy were activated in the others. This could possibly be related to the steady or improved cellular activity in both the organs, which might result in the life extension of these rats.

    DOI： 10.1016/j.heliyon.2017.e00462

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  • 八木沼 洋行, 松村 讓兒, 藤山 文乃, 中村 桂一郎, 網塚 憲生, 一條 裕之, 瀬藤 光利, 柴田 昌宏, 渡辺 雅彦 .  若手育成に関するアンケート調査の結果報告(2017年3月) .  解剖学雑誌92   2 - 8   2017.9若手育成に関するアンケート調査の結果報告(2017年3月)

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    Publisher：(一社)日本解剖学会  

    日本解剖学会教育・若手育成委員会は、研究医の養成に関する取組の実施状況と、若手研究者のキャリアアップを解剖学会としてどのように支援すべきかについての会員の意向を知ることを目的としてアンケート調査を行った。研究医の養成に関する取組で、この5年間の変化で特筆すべきことは、希望する学生を対象とした研究医養成コースの実施が、医学部では半数以上、歯学部でも4分の1程度までに増えており、履修者も多い状況となっていることである。多くの場合、このコースの履修によって大学院の履修期間の短縮、入学金や授業料の減免、奨学会や学会参加費の支援が行われ、さらに、大学間の連携で研修やリトリートも行われている。分野別認証評価導入の影響については、過度の臨床中心のカリキュラムを懸念する回答が多かったが、良い結果となったとする回答もあった。新専門医制度の導入については、回答者の約6割が、研究医を目指す人材がさらに少なくなることを懸念していた。今後の若手育成における学会の役割については、以下のような意見が寄せられた。(1)これまでの取組の維持・発展、(2)表彰制度の拡充、(3)経済的支援や負担軽減、(4)若手間や他分野の研究者との交流促進(若手の会の結成)、(5)研究情報交流の促進、(6)教育能力向上のための各種セミナーや合宿の実施、(7)教育能力に関する認定制度の導入、(8)キャリアアップ支援の強化、(9)その他。(著者抄録)

  • Masato Koike, Ai Kawahara, Masahiro Shibata, Yasuo Uchiyama .  Induction of Autophagy in the Hippocampus after Hypoxic Ischemic Injury to Neonatal Rats .  Archives of Histology and Cytology77 ( 1 ) 13 - 23   2017Induction of Autophagy in the Hippocampus after Hypoxic Ischemic Injury to Neonatal Rats Reviewed

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  • Masato Koike, Masahiro Shibata, Takehiko Sunabori, Junji Yamaguchi, Kenji Sakimura, Masaaki Komatsu, Keiji Tanaka, Yasuo Uchiyama .  Purkinje Cells Are More Vulnerable to the Specific Depletion of Cathepsin D Than to That of Atg7 .  American Journal of Pathology187 ( 7 ) 1586 - 1600   2017Purkinje Cells Are More Vulnerable to the Specific Depletion of Cathepsin D Than to That of Atg7Invited Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE INC  

    Neurologic phenotypes of cathepsin D (CTSD)-deficient mice, a murine model of neuronal ceroid lipofuscinoses, indicate the importance of CTSD for the maintenance of metabolism in central nervous system neurons. To further understand the role of CTSD in central nervous system neurons, we generated mice with a CTSD deficiency specifically in the Purkinje cells (PCs) (CTSD ;GRID2-Cre) and compared their phenotypes with those of PC-selective Atg7-deficient (Atg7 ;GRID2-Cre) mice. In both strains of mice, PCs underwent degeneration, but the CTSD-deficient PCs disappeared more rapidly than their Atg7-deficient counterparts. When CTSD-deficient PCs died, the neuronal cell bodies became shrunken, filled with autophagosomes and autolysosomes, and had nuclei with dispersed small chromatin fragments. The dying Atg7-deficient PCs also showed similar ultrastructures, indicating that the neuronal cell death of CTSD- and Atg7-deficient PCs was distinct from apoptosis. Immunohistochemical observations showed the formation of calbindin-positive axonal spheroids and the swelling of vesicular GABA transporter–positive presynaptic terminals that were more pronounced in Atg7-deficient PCs than in CTSD-deficient PCs. An accumulation of tubular vesicles may have derived from the smooth endoplasmic reticulum; nascent autophagosome-like structures with double membranes was a common feature in the swollen axons of these PCs. These results suggested that PCs were more vulnerable to CTSD deficiency in lysosomes than to autophagy impairment, and this vulnerability does not depend on the severity of axonal swelling. Flox/Flox Flox/Flox

    DOI： 10.1016/j.ajpath.2017.02.020

    Web of Science

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  • Masato Koike, Ai Kawahara, Masahiro Shibata, Yasuo Uchiyama .  Induction of autophagy in the hippocampus after hypoxic-ischemic injury to neonatal rats .  Archives of Histology and Cytology77 ( 1 ) 13 - 23   2017Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：International Society of Histology & Cytology  

    Neonatal hypoxic/ischemic (H/I) brain injury causes neurological impairment, including cognitive and motor dysfunction as well as seizures. Patterns of H/I injury-induced neuron death using rodent models are considered to be similar to the cases in human H/I encephalopathy. The participation of autophagy in neuron death has been a common feature in neonatal rodent models of H/I brain injury and human H/I encephalopathy when examined by immunochemical approaches for MAP1-LC3. This tendency has also been confirmed in neuronal tissue-specific Atg7 conditional knockout mice. However, while the current rat H/I model that is used for analyzing autophagy results in global damage to the ipsilateral hemisphere, it does not entirely reflect the neuropathological changes that appear in the neonatal mouse H/I model, in which the hippocampus is selectively damaged. The present study established a neonatal rat model of H/I injury with a milder ischemic insult, in which autophagy was involved in the hippocampal CA1 region after H/I injury when examined by electron microscopy, and by immunohistochemical and biochemical analyses of LC3.

    DOI： 10.1679/aohc.77.13

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  • Nagashima H, Sugahara F, Watanabe K, Shibata M, Chiba A, Sato N .  Developmental origin of the clavicle, and its implications for the evolution of the neck and the paired appendages in vertebrates .  Journal of Anatomy229 ( 4 ) 536 - 548   2016.10Developmental origin of the clavicle, and its implications for the evolution of the neck and the paired appendages in vertebratesReviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Mehanna S, Suzuki C, Shibata M, Sunabori T, Imanaka T, Araki K, Yamamura K, Uchiyama Y, Ohmuraya M .  Cathepsin D in pancreatic acinar cells is implicated in cathepsin B and L degradation, but not in autophagic activity .  Biochem Biophys Res Commun469 ( 3 ) 405 - 411   2016.1Cathepsin D in pancreatic acinar cells is implicated in cathepsin B and L degradation, but not in autophagic activityReviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Shibata M, Koike M, Kusumi S, Sato N, Uchiyama Y .  A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidney .  Arch Histol Cytol   2016A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidneyReviewed

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  • Nagashima H, Shibata M, Taniguchi M, Ueno S, Kamezaki N, Sato N .  Comparative study of the shell development of hard- and soft-shelled turtles. .  J Anat.225 ( 1 ) 60 - 70   2014Comparative study of the shell development of hard- and soft-shelled turtles.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Ohkouchi S, Shibata M, Sasaki M, Koike M, Safig P, Peters C, Nagata S, Uchiyama Y .  Biogenesis and proteolytic processing of lysosomal DNase II. .  PLoS One8 ( 3 )   2013Biogenesis and proteolytic processing of lysosomal DNase II.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Hayakawa N, Shiozaki M, Shibata M, Koike M, Uchiyama Y, Matsuura N, Gotow T .  Resveratrol affects undifferentiated and differentiated PC12 cells differently,particularly with respect to possible differences in mitochondrial and autophagicfunctions .  Eur J Cell Biol.92 ( 1 ) 30 - 43   2013Resveratrol affects undifferentiated and differentiated PC12 cells differently,particularly with respect to possible differences in mitochondrial and autophagicfunctionsReviewed

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  • Koike M, Shibata M, Ezaki J, Peters C, Saftig P, Kominami E, Uchiyama Y .  Differences in expression patterns of cathepsin C/dipeptidyl peptidase I in normal, pathological and aged mouse central nervous system .  Eur J Neurosci.37 ( 5 ) 816 - 830   2013Differences in expression patterns of cathepsin C/dipeptidyl peptidase I in normal, pathological and aged mouse central nervous systemReviewed

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  • Matsuo T, Kawasaki K, Osada T, Sawahata H, Suzuki T, Shibata M, Miyakawa N,Nakahara K, Iijima A, Sato N, Kawai K, Saito N, Hasegawa I .  Intrasulcal electrocorticography in macaque monkeys with minimally invasive neurosurgical protocols. .  Front Syst Neurosci.5   2011.5Intrasulcal electrocorticography in macaque monkeys with minimally invasive neurosurgical protocols.Reviewed

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  • Yamazaki Y, Shibata M, Ushiki T, Isokawa K, Sato N .  Bilateral, asymmetric anomalies of the anterior bellies of digastric muscles. .  J Oral Sci.53 ( 4 ) 523 - 527   2011Bilateral, asymmetric anomalies of the anterior bellies of digastric muscles.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Shiozaki M, Hayakawa N, Shibata M, Koike M, Uchiyama Y, Gotow T .  Closer association of mitochondria with lipid droplets in hepatocytes and activation of Kupffer cells in resveratrol-treated senescence-accelerated mice .  Histochem Cell Biol.136 ( 4 ) 475 - 89   2011Closer association of mitochondria with lipid droplets in hepatocytes and activation of Kupffer cells in resveratrol-treated senescence-accelerated miceReviewed

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  • Shibata M, Yoshimura K, Tamura H, Ueno T, Nishimura T, Inoue T, Sasaki M, Koike M, Arai H, Kominami E, Uchiyama Y .  LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation. .  Biochem Biophys Res Commun.393 ( 2 ) 274 - 279   2010LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Tamura H, Shibata M, Koike M, Sasaki M, Uchiyama Y .  Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brains .  J Histochem Cytochem.58 ( 5 ) 443 - 453   2010Atg9A protein, an autophagy-related membrane protein, is localized in the neurons of mouse brainsReviewed

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  • Shibata M, Yoshimura K, Furuya N, Koike M, Ueno T, Komatsu M, Arai H, Tanaka K, Kominami E, Uchiyama Y .  The MAP1-LC3 conjugation system is involved in lipid droplet formation .  Biochem Biophys Res Commun.382 ( 2 ) 419 - 423   2009The MAP1-LC3 conjugation system is involved in lipid droplet formationReviewed

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  • Uchiyama Y, Koike M, Shibata M, Sasaki M .  Autophagic neuron death .  Methods Enzymol.453   33 - 51   2009Autophagic neuron deathReviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

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MISC

  • 【「切片SEM法」の基礎と生物学・医学生物学研究への応用】切片SEM法と連続切片SEM法

    久住 聡, 甲賀 大輔, 柴田 昌宏, 渡部 剛

    顕微鏡   55 ( 1 )   18 - 22   2020.4

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    Publisher：(公社)日本顕微鏡学会  

    切片SEM法は、新たな走査電子顕微鏡(SEM)イメージング技法であり、スライドガラスに貼り付けた樹脂包埋組織切片から反射電子(BSE)像を観察する手法である。この手法では、高度なミクロトーム技術を必要とすることなく、透過電子顕微鏡のような超薄切片像を取得することができる。そこで本稿では、私たちが独自に開発した3つの切片SEM法、1)準超薄切片から超薄像をイメージする手法(準超薄切片SEM法)、2)蛍光像と準超薄切片BSE像の相関顕微鏡観察法(CLEM法)、3)凍結薄切法(徳安法)との組み合わせ法、について紹介する。さらに、新たな3Dイメージング技法である「連続切片SEM法」についても紹介し、切片のSEMイメージング技術の可能性について考察する。(著者抄録)

Research Projects

  • Lipid metabolism in the LC3 knockout mice

    Grant number：17K08515  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Shibata Masahiro

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4810000 （ Direct Cost: \3700000 、 Indirect Cost：\1110000 ）

    Autophagy is one of the bulk degradation systems in cells. LC3 is localized in the autophagosomal membrane so that it has been considered as a marker protein for autophagy. In this study, I examined the LC3 function in lipid metabolism using LC3 knockout mice. Adipose tissue mass in the mice deficient for LC3 having high fat diet was suppressed rather than in the control mice, and the lipid droplets in the cells differentiated into the adipocytes decreased rather than those derived from the control cells. Autophagy in the LC3 knockout mice crossed with the cathepsin D knockout mice, in which autophagy is increased, was not suppressed. These results indicate that LC3 would be compensated with other molecules in the course of autophagy.