Faculty Profiles - YABUKI Akira

Papers - YABUKI Akira

Division display 21 - 40 of about 150 ／ All the affair displays >>

Yabuki A, Mitani S, Sawa M, Mizukami K, Fujiki M, Yamato O. . A comparative study of chronic kidney disease in dogs and cats: Induction of cyclooxygenases. . Res Vet Sci. 93 ( 2 ) 892 - 897 2012.10A comparative study of chronic kidney disease in dogs and cats: Induction of cyclooxygenases. Reviewed

Yabuki A, Mitani S, Mizukami K, Yamato O. . Nephron segment identification in the normal canine kidney by using lectin histochemistry. . Res Vet Sci. 93 ( 2 ) 560 - 564 2012.10Nephron segment identification in the normal canine kidney by using lectin histochemistry.Reviewed

矢吹映、土岐学司、大和修 . 腎病理から逆流性腎症の進行による末期腎不全と診断された若齢犬の1例 . 日本獣医腎泌尿器学会誌 2011.11腎病理から逆流性腎症の進行による末期腎不全と診断された若齢犬の1例Reviewed

Ichii O, Yabuki A, Sasaki N, Otsuka S, Ohta H, Yamasaki M, Takiguchi M, Namiki Y, Hashimoto Y, Endoh D, Kon Y. . Pathological correlations between podocyte injuries and renal functions in canine and feline chronic kidney diseases. . Histol Histopathol. 2011 Oct;26(10):1243-55.26 ( 10 ) 1243 - 1255 2011.10Pathological correlations between podocyte injuries and renal functions in canine and feline chronic kidney diseases.Reviewed

Akira Yabuki, KeijiroMizukami,Mariko Sawa, SawaneMitani, and Osamu Yamato . Early Stage of Chronic Kidney Disease with Renal Injury Caused by Hypertension in a Dog . Case Reports in Veterinary Medicine 2011.8Early Stage of Chronic Kidney Disease with Renal Injury Caused by Hypertension in a DogReviewed

矢吹映、澤真理子 . 一次診療におけるGFRの測定 . J Vet 2011.4一次診療におけるGFRの測定

Yabuki A, Taniguchi K, Yamato O. . Immunohistochemical examination of cyclooxygenase-2 and renin in a KK-A(y) mouse model of diabetic nephropathy . Exp Anim59 ( 4 ) 479 - 486 2010.6Immunohistochemical examination of cyclooxygenase-2 and renin in a KK-A(y) mouse model of diabetic nephropathyReviewed

矢吹映 . 尿路閉塞と急性腎後性高窒素血症 . J VET280 21 - 31 2010.6尿路閉塞と急性腎後性高窒素血症

Yabuki A, Mitani A, Fujiki M, Misumi K, Endo Y, Miyoshi N, Yamato O. . Comparative study of chronic kidney disease in dogs and cats: Induction of myofibroblasts. . Res Vet Sci 88 ( 2 ) 294 - 299 2010.4Comparative study of chronic kidney disease in dogs and cats: Induction of myofibroblasts. Reviewed

矢吹映、川崎安亮、池田耕夫、大和修 . 巣状糸球体硬化症の進行による末期腎不全と診断されたイヌの１例 . 日本獣医腎泌尿器学会誌2 46 - 50 2010.2巣状糸球体硬化症の進行による末期腎不全と診断されたイヌの１例Reviewed

Fujimoto M., Takahashi M., Hifumi T., Yabuki A., Furusawa Y., Hatai H., Endo Y. . Cabot rings in a cat with myeloproliferative disease . Veterinary Clinical Pathology53 ( 2 ) 196 - 201 2024.6Reviewed

Language：Japanese Publisher：Veterinary Clinical Pathology

A 6-year-old spayed female Scottish Fold cat presented with lethargy and anorexia. A complete blood cell count indicated severe anemia and mild thrombocytopenia. Examination of peripheral blood smears revealed marked changes in the erythroid lineage, including the presence of basophilic stippling and Howell-Jolly bodies as well as an increase in nucleated erythrocytes, polychromatophils, ovalocytes, and schistocytes. Additionally, some erythrocytes contained a ring or figure-eight shaped structure known as a Cabot ring, which were especially observed in polychromatophilic erythrocytes. Hemolytic diseases (Mycoplasma infection and IMHA) were diagnostically excluded, and the cat was treated through prednisolone administration, whole blood transfusion, and administration of vitamins (K2 and B12); however, the anemia progressively worsened. Cabot rings were observed until Day 22 and subsequently disappeared as the number of nucleated RBCs increased, and the erythrocyte lineage shifted to immature population. On Day 42, peripheral blood examination revealed further left shifting and appearance of many rubriblasts. The patient died at home on Day 43. Necropsy revealed neoplastic cells infiltrating the bone marrow and other organs, which were immunopositive to CD71 which is an erythroid lineage marker. In humans, Cabot rings have been observed in megaloblastic anemia, lead poisoning, myelodysplastic syndrome, and myelofibrosis; further, they are thought to be related to stressed bone marrow and dyserythropoiesis. This is the first case report of a cat with Cabot rings, which are suggestive of defects in erythroid lineage production.

DOI： 10.1111/vcp.13340

Scopus

PubMed

Rakib T.M., Islam M.S., Uddin M.M., Rahman M.M., Yabuki A., Yamagami T., Morozumi M., Uchida K., Maki S., Faruq A.A., Yamato O. . Novel Mutation in the Feline NPC2 Gene in Cats with Niemann–Pick Disease . Animals13 ( 11 ) 2023.6

Language：Japanese Publisher：Animals

Niemann–Pick disease (NP) type C is an autosomal, recessive, and inherited neurovisceral genetic disorder characterized by the accumulation of unesterified cholesterol and glycolipids in cellular lysosomes and late endosomes, with a wide spectrum of clinical phenotypes. This study aimed to determine the molecular genetic alterations in two cases of felines with NP in Japan, a Siamese cat in 1989 and a Japanese domestic (JD) cat in 1998. Sanger sequencing was performed on 25 exons of the feline NPC1 gene and 4 exons of the feline NPC2 gene, using genomic DNA extracted from paraffin-embedded tissue specimens. The sequenced exons were compared with reference sequences retrieved from the GenBank database. The identified mutations and alterations were then analyzed using different prediction algorithms. No pathogenic mutations were found in feline NPC1; however, c.376G>A (p.V126M) was identified as a pathogenic mutation in the NPC2 gene. The Siamese cat was found to be homozygous for this mutation. The JD cat was heterozygous for the same mutation, but no other exonic NPC2 mutation was found. Furthermore, the JD cat had a homozygous splice variant (c.364-4C>T) in the NPC2 gene, which is not known to be associated with this disease. The NPC2:c.376G>A (p.V126M) mutation is the second reported pathogenic mutation in the feline NPC2 gene that may be present in the Japanese cat population.

DOI： 10.3390/ani13111744

Scopus

PubMed

Rakib T.M., Islam M.S., Tanaka S., Yabuki A., Pervin S., Maki S., Faruq A.A., Tacharina M.R., Yamato O. . Novel Mutation in the Feline GAA Gene in a Cat with Glycogen Storage Disease Type II (Pompe Disease) . Animals13 ( 8 ) 2023.4

Language：Japanese Publisher：Animals

Glycogen storage disease type II (Pompe disease: PD) is an autosomal recessively inherited fatal genetic disorder that results from the deficiency of a glycogen hydrolyzing enzyme, acid α-glucosidase encoded by the GAA gene. Here, we describe the molecular basis of genetic defects in an 8-month-old domestic short-haired cat with PD. The cat was previously diagnosed with PD based on the clinical and pathological findings of hypertrophic cardiomyopathy and excessive accumulation of glycogen in the cardiac muscles. Sanger sequencing was performed on 20 exons of the feline GAA gene using genomic DNA extracted from paraffin-embedded liver tissues. The affected cat was found to be homozygous for the GAA:c.1799G>A mutation resulting in an amino acid substitution (p.R600H) of acid α-glucosidase, a codon position of which is identical with three missense mutations (p.R600C, p.R600L, and p.R600H) causing human infantile-onset PD (IOPD). Several stability and pathogenicity predictors have also shown that the feline mutation is deleterious and severely decreases the stability of the GAA protein. The clinical, pathological, and molecular findings in the cat were similar to those of IOPD in humans. To our knowledge, this is the first report of a pathogenic mutation in a cat. Feline PD is an excellent model for human PD, especially IOPD.

DOI： 10.3390/ani13081336

Scopus

PubMed

Furusawa Y., Shima-Sawa M., Hifumi T., Miyoshi N., Yamato O., Yabuki A. . Usefulness and Limitations of Cryopreservation for Immunocytochemical Staining of Canine Cytological Specimens for Detection of Cytokeratin and Vimentin . Veterinary Sciences10 ( 2 ) 2023.2

Language：Japanese Publisher：Veterinary Sciences

Immunocytochemistry is an advanced diagnostic tool for identifying the origin of tumor cells. This study aimed to highlight the usefulness of cryopreserved, air-dried cytological samples in detecting cytokeratin and vimentin. Air-dried cytological smear samples were prepared from a total of 39 resected canine tumors and stored in a medical freezer without fixation. The duration of cryopreservation ranged from 2 to 56 months. The same tumors were processed for routine histopathological examination. Based on the morphological diagnosis, cryopreserved FNA smears from epithelial tumors were stained by enzymatic immunocytochemistry (ICC) for cytokeratin; those from mesenchymal and melanocytic tumors were stained by ICC for vimentin. To ascertain the positivity of tumor cells to the selected markers, tissue paraffin-embedded sections were also stained by immunohistochemistry (IHC) for the same markers. Immunoreactivity for cytokeratin was detected in cryopreserved cytological smears for a maximum of 46 months. Immunoreactivity for vimentin was clearly detected for 33 months. Smears stored at room temperature for 1 week did not show any signals under immunocytochemical examination. Thus, immunocytochemistry for cytokeratin and vimentin can be safely applied to air-dried smears cryopreserved in a freezer for at least 33 months.

DOI： 10.3390/vetsci10020153

Scopus

PubMed

Takahashi K., Nango H., Ushijima M., Takashima M., Nakamoto M., Matsutomo T., Jikihara H., Arakawa N., Maki S., Yabuki A., Endo Y., Yamato O. . Therapeutic effect of aged garlic extract on gingivitis in dogs . Frontiers in Veterinary Science10 1277272 2023

Language：Japanese Publisher：Frontiers in Veterinary Science

Periodontal disease is one of the most common dental health problems in dogs. Clinical studies in humans have shown that aged garlic extract (AGE), which contains stable and water-soluble sulfur-containing bioactive compounds, improves the symptoms of periodontal diseases. Our previous study demonstrated that oral administration of AGE in healthy Beagle dogs at 90 mg/kg/day for 12 weeks had no adverse effects such as hemolytic anemia, which is well known to occur as a result of ingestion of Allium species, including onions and garlic, in dogs. However, the therapeutic potential of AGE in canine periodontal disease remains unclear. Accordingly, we investigated the therapeutic effects of AGE in Beagle dogs with mild gingivitis. Feeding 18 mg/kg/day of AGE for 8 weeks resulted in the improvement of gingival index score, level of volatile sulfur compounds in exhaled air, and enzyme activity of periodontal pathogens without any adverse effects on clinical signs and hematological and serum biochemical parameters. Moreover, AGE increased the concentration of salivary cathelicidin, an antimicrobial peptide that contributes to the oral innate immune response. These results suggest that AGE could be a potential therapeutic agent for canine gingivitis.

DOI： 10.3389/fvets.2023.1277272

Scopus

PubMed

Maki S., Islam M.S., Itoh T., Nurimoto M., Yabuki A., Furusawa Y., Kamishina H., Kobatake Y., Rakib T.M., Tacharina M.R., Yamato O. . Molecular Epidemiological Survey for Degenerative Myelopathy in German Shepherd Dogs in Japan: Allele Frequency and Clinical Progression Rate . Animals12 ( 13 ) 2022.7

Language：Japanese Publisher：Animals

Canine degenerative myelopathy (DM) is an adult-onset, chronic, progressive neurode-generative disease reported in multiple canine breeds, including the German Shepherd Dog (GSD). Clinical signs include progressive motor neuron paralysis, which begins in the pelvic limbs and eventually leads to respiratory distress, which may necessitate euthanasia. A common DM-associated mutation is a single nucleotide substitution that causes an amino acid substitution (c.118G>A, p.E40K) in the canine SOD1 gene. This SOD1 mutation and the clinical progression rate of A/A risk genotype in the Japanese GSD population have not been analyzed before. Therefore, the aim of this study was to determine the frequency of the mutated allele and analyze the clinical progression rate in the Japanese GSD population. We studied 541 GSDs registered with the Japanese German Shepherd Dog Registration Society between 2000 and 2019. Genotyping was performed using real-time PCR with DNA extracted from the hair roots of each dog. The study revealed 330 G/G dogs (61%), 184 G/A dogs (34%), and 27 A/A dogs (5%), indicating a frequency of the mutant allele of 0.220, which are in Hardy–Weinberg equilibrium. We analyzed the clinical signs in A/A dogs with an age limit of 10 years based on information obtained from the dogs’ owners. Of the seven A/A dogs older than 10 years, owners reported DM-related clinical signs, indicating a clinical progression rate of 100%. These results, further genotyping, and thorough clinical examinations of SOD1 A/A risk genotype will help control and prevent DM in the Japanese GSD population.

DOI： 10.3390/ani12131647

Scopus

PubMed

Pervin S, Islam MS, Yorisada Y, Sakai A, Masamune S, Yabuki A, Rakib TM, Maki S, Tacharina MR, Yamato O . Carrier Rate and Mutant Allele Frequency of GM1 Gangliosidosis in Miniature Shiba Inus (Mame Shiba): Population Screening of Breeding Dogs in Japan. . Animals : an open access journal from MDPI12 ( 10 ) 2022.5Carrier Rate and Mutant Allele Frequency of GM1 Gangliosidosis in Miniature Shiba Inus (Mame Shiba): Population Screening of Breeding Dogs in Japan.

Pervin S., Islam M.S., Tada N., Tsutsui T., Rahman M.M., Yabuki A., Tacharina M.R., Rakib T.M., Maki S., Yamato O. . Screening and Carrier Rate of Neuronal Ceroid Lipofuscinosis in Chihuahua Dogs in Japan . Animals12 ( 9 ) 2022.5

Language：Japanese Publisher：Animals

Neuronal ceroid lipofuscinosis (NCL) is a group of rare lethal neurodegenerative lysosomal storage diseases that occur in a range of dog breeds, including Chihuahuas. Recently, a homozygous single base-pair deletion (c.846delT), which causes a frame shift generating a premature stop codon (p.Phe282Leufs13*) in the canine CLN7/MFSD8 gene, has been identified as a causative mutation for NCL in Chihuahuas. The objective of this study was to determine the frequency of the mutant allele and/or carrier rate of NCL in Chihuahuas in Japan using a newly designed real-time PCR assay. Samples of saliva were randomly collected from 1007 Chihuahua puppies during physical examinations prior to the transportation to pet shops. Screening results revealed a carrier rate of 1.29%, indicating a mutant allele frequency (0.00645) that is considered sufficiently high to warrant measures for the control and prevention of this lethal disease. The genotyping assay designed in this study could make a valuable contribution to the control and prevention of NCL.

DOI： 10.3390/ani12091210

Scopus

PubMed

Islam M.S., Takagi M., Lee K.W., Chang H.S., Okawa H., Yunus M., Lestari T.D., Tacharina M.R., Pervin S., Rakib T.M., Yabuki A., Yamato O. . Frequency of an X-Linked Maternal Variant of the Bovine FOXP3 Gene Associated with Infertility in Different Cattle Breeds: A Pilot Study . Animals12 ( 8 ) 2022.4

Language：Japanese Publisher：Animals

Immune adaptation plays an essential role in determining pregnancy, which has been shown to be dependent on sufficient immunological tolerance mediated by FOXP3+ regulatory T cells. Recently, an X-linked maternal single-nucleotide polymorphism (SNP), located 2175 base pairs upstream of the start codon in the bovine FOXP3 gene (NC_037357.1: g.87298881A>G, rs135720414), was identified in Japanese Black (JB: Bos taurus) cows in association with recurrent infertility. However, with the exception of JB cows, the frequency of this SNP has yet to be studied in other cow populations. In this study, we thus aimed to evaluate the frequency of this SNP in different cow breeds. Between 2018 and 2021, a total of 809 DNA samples were obtained from 581 JB, 73 Holstein Friesian (HF: B. taurus), 125 Korean Hanwoo (KH: B. taurus coreanae), and 30 Indonesian Madura (IM: a crossbreed between B. indicus and B. javanicus) cows, which were genotyped using a TaqMan probe-based real-time polymerase chain reaction assay designed in this study. The frequency of the G allele was found to be relatively high in local IM (0.700), moderate in dairy HF (0.466), and low in beef JB (0.250) and KH (0.112) cows, with differences in the frequencies between each group being shown to be statistically significant (p < 0.005) using Fisher’s exact test. The results obtained in this study indicate that the G allele frequencies of the identified the SNP differ markedly in different breeds of taurine and indicine cattle. Given these findings, it would thus be important to evaluate the relationships between high frequencies of the G allele and infertility in different breeds.

DOI： 10.3390/ani12081044

Scopus

PubMed

Tanaka S., Suzuki R., Koyama H., Machida N., Yabuki A., Yamato O. . Glycogen storage disease in a young cat with heart failure . Journal of Veterinary Internal Medicine36 ( 1 ) 259 - 263 2022.1

Language：Japanese Publisher：Journal of Veterinary Internal Medicine

An 8-month-old domestic short-haired female cat presented with acute tachypnea, poor growth, hypothermia, and lethargy. Thoracic radiography showed cardiomegaly with mild pleural effusion, and transthoracic echocardiography identified dilatation of both atria and left ventricular systolic dysfunction. Although clinical signs improved temporarily with treatment, the cat died of pulmonary edema 135 days after the first visit. At necropsy, the heart was grossly enlarged. Microscopic examination of the heart identified severe vacuolization of cardiac muscle cells in histologic sections stained with hematoxylin and eosin. Examination of periodic acid-Schiff stained preparations of formalin-fixed heart tissue disclosed coarse granules within vacuoles that disappeared on predigestion with diastase, indicating that they were glycogen. On the basis of these findings, a necropsy diagnosis of glycogen storage disease type II (Pompe disease) was made. This report is the first case of a young cat with clinical signs closely resembling infantile Pompe disease of humans.

DOI： 10.1111/jvim.16339

Scopus

PubMed

　 PREV - NEXT 　

<YABUKI Akira>

Papers - YABUKI Akira