Faculty Profiles - YABUKI Akira

Papers - YABUKI Akira

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矢吹映 . 腎生検の実際　腎臓の解剖、腎組織の採取と取り扱い . 日本獣医腎泌尿器学会誌15 ( 1 ) 4 - 9 2024.2腎生検の実際　腎臓の解剖、腎組織の採取と取り扱いReviewed

Authorship：Lead author,　Corresponding author Language：Japanese Publisher：日本獣医腎泌尿器学会

腎生検は、腎疾患、特に蛋白漏出性腎症の原因となる糸球体疾患の診断に必須の診断法である。しかしながら、腎生検は侵襲的な検査法であり安易に実施するものではない。また、診断には一般的な光学顕微鏡検査だけでなく電子顕微鏡や蛍光抗体法が必要であり、採取した腎臓組織の取り扱いにも相応の知識と準備が必要である。本稿では、腎生検を安全に実施し、良質な組織を採取するための腎臓の解剖とサンプリング法、組織の取扱法について解説する。(著者抄録)

YABUKI Akira, ANDO Takanori, TANABE Mika, KURAHARA Natsume, YAMATO Osamu . Severe glomerular fibrin thrombosis in a dog . Journal of Veterinary Medical Science86 ( 1 ) 87 - 91 2024Reviewed

Language：English Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE

<p>Here, we present a case of severe glomerular fibrin thrombosis in a dog with lymphoma. A 3-year-old neutered male Chihuahua presented with acute kidney injury, hypoalbuminemia, and transudate ascites. The dog showed symmetric enlargement of the spleen, which was diagnosed as B-cell lymphoma based on cytology and polymerase chain reaction tests. The dog died after intensive care, and the kidneys were removed for histopathological examination. Light microscopy, immunofluorescence, and electron microscopy analyses were performed for renal pathology; however, the findings did not support the evidence of protein-losing nephropathy. Instead, the endocapillary accumulation of fibrin thrombi was prominent in most glomeruli. A diagnosis of severe glomerular fibrin thrombosis was established, and hypoalbuminemia was considered the underlying cause of kidney damage.</p>

DOI： 10.1292/jvms.23-0388

Scopus

PubMed

Kurahara N., Yutsudo A., Furusawa Y., Yamato O., Miyoshi N., Hifumi T., Yabuki A. . Immunohistochemical analysis of renal oxidative damage in senior and geriatric cats with chronic kidney disease . Journal of Comparative Pathology207 14 - 17 2023.11Reviewed

Language：Japanese Publisher：Journal of Comparative Pathology

Oxidative stress is a well-known cause of chronic kidney disease (CKD). In this study, renal oxidative damage in azotaemic and non-azotaemic aged cats with naturally occurring CKD was investigated using immunohistochemistry for 8-hydroxy-2′-deoxyguanosine (8-OHdG) and 4-hydroxynonenal (4-HNE) as markers of oxidative tissue damage. Kidneys were obtained from aged (>10 years old) azotaemic (n = 13) and non-azotaemic (n = 7) cats. Immunoreactivity for 8-OHdG was found in the nuclei of glomeruli, proximal and distal tubules, loops of Henle and collecting ducts, whereas 4-HNE-positive signals were detected in the cytoplasm of distal nephrons in azotaemic and non-azotaemic cats. Quantitative analysis did not identify any significant differences between the azotaemic and non-azotaemic groups for any of the parameters examined. These results indicate that renal oxidative damage occurs in the kidneys of aged cats with CKD, regardless of whether they are azotaemic or non-azotaemic, emphasizing the importance of oxidative stress during early-stage CKD in senior and geriatric cats.

DOI： 10.1016/j.jcpa.2023.09.001

Scopus

PubMed

Noguchi A., Kurahara N., Yamato O., Ichii O., Yabuki A. . Lectin Histochemistry of the Normal Feline Kidney . Veterinary Sciences10 ( 1 ) 2023.1Reviewed

Language：Japanese Publisher：Veterinary Sciences

Lectins have a strict binding specificity to carbohydrate moieties of cellular components, and can thus indicate changes in the glycosylation of cells in diseases. However, lectin-binding patterns in nephron segments of feline kidneys have not been fully surveyed. The present study reported lectin-binding patterns in normal feline kidneys by histochemical investigations of eight commercially available lectin detection kits. Kidneys from four normal cats (intact males, 23–27 months old) were fixed in 4% paraformaldehyde, and embedded in paraffin; lectin histochemistry was performed for WGA, s-WGA, RCA-I, ConA, PNA, SBA, DBA, and UEA-I lectins. WGA, RCA, and ConA binding was observed from Bowman’s capsule to the collecting ducts, while only WGA was detected in the glomerular capillary. s-WGA was observed from the proximal tubules to the collecting ducts, showing discriminative heterogeneous binding. PNA and SBA were detected in the distal nephrons, such as the thin limbs of the loops of Henle, distal tubules, and collecting ducts. UEA-I binding was observed in the thick ascending limbs of the loops of Henle, especially in the macula densa regions. DBA lectin showed no positive labeling in nephrons. The observed binding patterns may prove beneficial for the analysis of changes in glycosylation in feline kidney diseases.

DOI： 10.3390/vetsci10010026

Scopus

PubMed

Uehara Y., Furusawa Y., Islam M.S., Yamato O., Hatai H., Ichii O., Yabuki A. . Immunohistochemical Expression of TGF-β1 in Kidneys of Cats with Chronic Kidney Disease . Veterinary Sciences9 ( 3 ) 2022.3Reviewed

Language：Japanese Publisher：Veterinary Sciences

Transforming growth factor-beta 1 (TGF-β1) plays a central role in the progression of chronic kidney disease (CKD). However, in feline CKD, renal expression of TGF-β1 and how it changes as the disease progresses have not been fully studied. In the present study, we immunohistochemically assessed the renal expression levels of TGF-β1 in cats with CKD and statistically analyzed its correlation with CKD severity. Clear immunosignals were detected in the glomerular mesangial cells, Bowman’s capsules, proximal tubules, distal nephrons, platelets, and vascular smooth muscles in the kidneys of cats with CKD. Statistically, luminal signals in the distal nephrons showed positive correlations with plasma creatinine levels and glomerulosclerosis, while those in the proximal tubules and platelets showed negative correlations with plasma urea and/or creatinine levels. Therefore, it was suggested that the changes in the renal expression of TGF-β1 could be associated with progression of feline CKD.

DOI： 10.3390/vetsci9030114

Scopus

PubMed

YABUKI Akira, FURUSAWA Yu, MIYOSHI Noriaki, TANIGUCHI Kazuyuki, YAMATO Osamu . Expression of neuronal nitric oxide synthase and renin in dysplastic kidneys of young dogs . Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 2021Reviewed

Publisher：公益社団法人日本獣医学会

<p>Renin and neuronal nitric oxide synthase in the kidney control the renin-angiotensin and tubuloglomerular feedback systems. The present study investigated the expression of renin and neuronal nitric oxide synthase in the dysplastic kidneys of three young dogs. Renin-immunoreactivity, which occurs in the juxtaglomerular and tubular cells of dysplastic kidneys, did not differ from that in the normal kidneys of young dogs. Macula densa cells in the normal kidneys showed neuronal nitric oxide synthase -immunoreactivity, but those in the dysplastic kidneys showed no apparent signals. This observation may be correlated with the pathological mechanisms of renal failure in young dogs.</p>

DOI： 10.1292/jvms.21-0074

PubMed

YABUKI Akira . Urinalysis . JOURNAL OF JAPANESE ASSOCIATION OF VETERINARY NEPHROLOGY AND UROLOGY13 ( 1 ) 3 - 6 2021

Yabuki A, Uehara Y, Ichii O, Yoshida C, Yamato O . Expression of Peroxisome Proliferator-activated Receptor-γ in the Kidneys of Cats with Chronic Kidney Disease . Journal of Comparative Pathology176 81 - 85 2020.4Expression of Peroxisome Proliferator-activated Receptor-γ in the Kidneys of Cats with Chronic Kidney DiseaseReviewed

Ohara Y. . Renal Infiltration of Macrophages in Canine and Feline Chronic Kidney Disease . Journal of Comparative Pathology170 53 - 59 2019.7Reviewed

Yabuki A, Hamamoto T, Rahman MM, Yamato O . Changes in lectin-binding patterns in the kidneys of canines with immune-complex mediated glomerulonephritis . J Vet Med Sci80 ( 10 ) 1562 - 1566 2018.11Changes in lectin-binding patterns in the kidneys of canines with immune-complex mediated glomerulonephritisReviewed

Nakamura R, Yabuki A, Ichii O, Mizukawa H, Yokoyama N, Yamato O . Changes in Renal Peritubular Capillaries in Canine and Feline Chronic Kidney Disease . J Comp Pathol160 79 - 83 2018.4Changes in Renal Peritubular Capillaries in Canine and Feline Chronic Kidney DiseaseReviewed

Yabuki A, Sawa M, Kohyama M, Hamamoto T, Yamato O . Paraffin immunofluorescence for detection of immune complexes in renal biopsies: an efficient salvage technique for diagnosis of glomerulonephritis in dogs . BMC Vet Res13 ( 1 ) 371 2017.12Paraffin immunofluorescence for detection of immune complexes in renal biopsies: an efficient salvage technique for diagnosis of glomerulonephritis in dogsReviewed

Yabuki A, Iwanaga T, Giger U, Sawa M, Kohyama M, Yamato O . Acquired Fanconi syndrome in two dogs following long-term consumption of pet jerky treats in Japan: case report . J Vet Med Sci79 818 - 821 2017.5Acquired Fanconi syndrome in two dogs following long-term consumption of pet jerky treats in Japan: case reportReviewed

Yabuki A, Miyazaki A, Ichii O, Kohyama M, Sawa M, Yamato O . Low expression of cyclooxygenase-2 in chronic kidney disease in young dogs . Res Vet Sci109 71 - 73 2016.12Low expression of cyclooxygenase-2 in chronic kidney disease in young dogsReviewed

Yabuki A, Shimokawa Miyama T, Kohyama M, Yamato O. . Canine IgA nephropathy: a case report . 2016.4Canine IgA nephropathy: a case reportReviewed

Yabuki A, Mizukami K, Tokunaga S, Yamato O . Change in intrarenal Ghrelin expression in immune complex-mediated glomerular disease in dogs . Journal of Veterinary Medical Science 2015.8Change in intrarenal Ghrelin expression in immune complex-mediated glomerular disease in dogsReviewed

矢吹映、宮豊、宮紀子、野添伸樹、大和修 . 糸球体腎炎と膵炎を併発した犬の3例－腎生検と治療経過について . 鹿児島県獣医師会会報52 23 - 25 2014.10糸球体腎炎と膵炎を併発した犬の3例－腎生検と治療経過について

Yabuki A, Yoneshige S, Tanaka S, Tsujio M, Mitani S, Yamato O. . Age-related histological changes in kidneys of Brown Norway rat. . J Vet Med Sci76 ( 2 ) 277 - 280 2014.3Age-related histological changes in kidneys of Brown Norway rat.Reviewed

Mitani S, Yabuki A, Sawa M, Chang HS, Yamato O. . Intrarenal distributions and changes of Angiotensin-converting enzyme and Angiotensin-converting enzyme 2 in feline and canine chronic kidney disease. . J Vet Med Sci76 ( 1 ) 45 - 50 2014.1Intrarenal distributions and changes of Angiotensin-converting enzyme and Angiotensin-converting enzyme 2 in feline and canine chronic kidney disease.Reviewed

Mitani S, Yabuki A, Taniguchi K, Yamato O. . Association between the Intrarenal Renin-Angiotensin System and Renal Injury in Chronic Kidney Disease of Dogs and Cats. . J Vet Med Sci75 ( 2 ) 127 - 133 2013.3Association between the Intrarenal Renin-Angiotensin System and Renal Injury in Chronic Kidney Disease of Dogs and Cats.Reviewed

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