2024/11/09 更新

写真a

シライシ ミツヤ
白石 光也
SHIRAISHI Mitsuya
所属
農水産獣医学域獣医学系 共同獣医学部 共同獣医学科 教授
職名
教授
連絡先
メールアドレス

学位

  • 博士(獣医学) ( 2001年3月   山口大学 )

研究キーワード

  • MARCKSタンパク質

  • 細胞内シグナル伝達

  • 重金属

  • 血小板

  • 血管内皮細胞

  • 神経細胞

研究分野

  • ライフサイエンス / 獣医学

学歴

  • 山口大学   獣医学

    1997年4月 - 2001年3月

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    国名: 日本国

  • 宮崎大学   獣医学科

    1991年4月 - 1997年3月

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    国名: 日本国

経歴

  • 鹿児島大学   農水産獣医学域獣医学系 共同獣医学部 獣医学科 基礎獣医学講座   教授

    2019年2月 - 現在

  • 鹿児島大学   農水産獣医学域獣医学系 共同獣医学部 獣医学科 基礎獣医学講座   准教授

    2012年4月 - 2019年1月

  • 鹿児島大学   農学部獣医学科   准教授

    2007年4月 - 2012年3月

  • 鹿児島大学   農学部獣医学科   助教授

    2007年3月

  • 北里大学   薬学部   助手

    2004年4月 - 2007年2月

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    国名:日本国

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所属学協会

  • 日本毒性学会

    2016年5月 - 現在

  • 日本薬理学会

    2007年3月 - 現在

  • 日本獣医学会

    2006年12月 - 現在

  • 日本生化学会

    2006年1月 - 現在

 

論文

  • Ferdous J., Naitou K., Shiraishi M. .  Distinct In Vitro Differentiation Protocols Differentially Affect Cytotoxicity Induced by Heavy Metals in Human Neuroblastoma SH-SY5Y Cells .  Biological Trace Element Research   2024年8月査読 国際誌

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Biological Trace Element Research  

    The SH-SY5Y cell line is widely used in neurotoxicity studies. However, the effects of inducing cell differentiation on the cytotoxic effects of heavy metals are unclear. Therefore, we investigated the effects of mercuric chloride (HgCl2), cadmium chloride (CdCl2), arsenic trioxide (As2O3), and methylmercury (MeHg) on SH-SY5Y cells differentiated in the presence of insulin-like growth factor-I (IGF-I) or all-trans retinoic acid (ATRA). Neurite outgrowth with distinct changes in neuronal marker expression, phenotype, and cell cycle was induced in SH-SY5Y cells by IGF-I treatment for 1 day or ATRA treatment for up to 7 days. The cytotoxic effects of HgCl2 decreased at lower concentrations and increased at higher concentrations in both IGF-I- and ATRA-differentiated cells compared with those in undifferentiated cells. Differentiation with IGF-I, but not with ATRA, increased the cytotoxic effects of CdCl2. Decreased cytotoxic effects of As2O3 and MeHg were observed at lower concentrations in IGF-I-differentiated cells, whereas increased cytotoxic effects of As2O3 and MeHg were observed at higher concentrations in ATRA-differentiated cells. Changes in the cytotoxic effects of heavy metals were observed even after 1 day of ATRA exposure in SH-SY5Y cells. Our results demonstrate that the differentiation of SH-SY5Y cells by IGF-I and ATRA induces different cellular characteristics, resulting in diverse changes in sensitivity to heavy metals, which depend not only on the differentiation agents and treatment time but also on the heavy metal species and concentration.

    DOI: 10.1007/s12011-024-04342-x

    Scopus

    PubMed

    その他リンク: https://link.springer.com/article/10.1007/s12011-024-04342-x/fulltext.html

  • Ootawa T., Wu S., Sekio R., Smith H., Islam M.Z., Nguyen H.T.T., Uno Y., Shiraishi M., Miyamoto A. .  Habu snakes (Protobothrops flavoviridis) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands .  Journal of Veterinary Medical Science86 ( 2 ) 202 - 206   2024年査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本獣医学会  

    Habu snakes (Protobothrops flavoviridis) are pit vipers found in the geographically adjacent but ecologically divergent islands of Tokunoshima and Amami-Oshima in southwestern Japan. Abiotic factors can cause variation in animal populations between the two islands, and Habu snakes may show such intraspecific physiological variation. We therefore evaluated the vasoreactivity in aortas isolated from the Habu of both islands. Tokunoshima Habu showed significantly greater contractile responses to angiotensin (Ang) II, acetylcholine (ACh) and noradrenaline, and significantly higher affinities (pEC50) for Ang II and ACh, than Amami-Oshima Habu. ACh caused contractions in aortas from both populations, a finding previously unreported in snakes. Our findings indicate that vasoreactivity may differ between Tokunoshima and Amami-Oshima Habu.

    DOI: 10.1292/jvms.23-0361

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    PubMed

  • Ferdous J, Naitou K, Shiraishi M .  A peptide against the N-terminus of myristoylated alanine-rich C kinase substrate promotes neuronal differentiation in SH-SY5Y human neuroblastoma cells. .  Journal of Veterinary Medical Scienceadvpub ( 0 ) 1136 - 1144   2024年査読

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本獣医学会  

    DOI: 10.1292/jvms.24-0276

    PubMed

  • Ootawa T., Wu S., Sekio R., Smith H., Islam M.Z., Nguyen H.T.T., Uno Y., Shiraishi M., Miyamoto A. .  Characterization of Vasoreactivity in a Semi-Arboreal Snake, the Tokara Habu (Protobothrops tokarensis) .  Animals13 ( 23 ) 3629 - 3629   2023年11月査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Animals  

    Vasoreactivity is relatively well documented in terrestrial snakes but has previously been investigated in only one semi-arboreal snake species. Consequently, the extent to which vasoreactivity is common across snake taxa or varies by habitat is unclear. The Tokara habu (Protobothrops tokarensis) is a semi-arboreal snake endemic to only two small adjacent Japanese islands, and hence a useful species for further investigation of vasoreactivity. We evaluated responses to known vasoactive substances in thoracic aortas isolated from Tokara habu. Under resting tension, noradrenaline and angiotensin II induced concentration-dependent contraction, but acetylcholine, serotonin (5-hydroxytriptamine; 5-HT), and isoproterenol induced relaxation followed by contraction. Histamine and rattlesnake bradykinin had no effect. Experiments with receptor-specific antagonists suggest that M1 and M3 receptors are involved in the acetylcholine-induced response; 5-HT1, 5-HT2, and 5-HT7 receptors in the serotonin-induced response; and β1 and β2 adrenoceptors in isoproterenol-induced relaxation. This is the first report on such response patterns in snakes (including serotonin- and isoproterenol-induced relaxation). Nitric oxide may be involved in acetylcholine-induced relaxation but not in the responses to serotonin or isoproterenol. In contrast to the uniform vasoreactivity observed in terrestrial snakes, the vasoreactivity of semi-arboreal snakes may be governed by diverse regulatory mechanisms.

    DOI: 10.3390/ani13233629

    Scopus

    PubMed

  • Wu S., Ootawa T., Sekio R., Smith H., Islam M.Z., Nguyen H.T.T., Uno Y., Shiraishi M., Miyamoto A. .  Reduced Nitric Oxide Synthase Involvement in Aigamo Duck Basilar Arterial Relaxation .  Animals13 ( 17 )   2023年9月査読

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Animals  

    The basilar arterial endothelium mediates blood vessel relaxation partly through the release of nitric oxide (NO). Apoptosis of cerebrovascular endothelial cells is linked to a high mortality rate in chickens infected with the highly pathogenic avian influenza virus, but interestingly, ducks exhibit a greater resistance to this virus. In this study, we examined the responsiveness of duck basilar arteries (BAs) to various vasoactive substances, including 5-hydroxytryptamine (5-HT), histamine (His), angiotensin (Ang) II, noradrenaline (NA), acetylcholine (ACh), and avian bradykinin ornithokinin (OK), aiming to characterize the receptor subtypes involved and the role of endothelial NO in vitro. Our findings suggest that arterial contraction is mediated with 5-HT1 and H1 receptors, while relaxation is induced with β3-adrenergic and M3 receptors. Additionally, OK elicited a biphasic response in duck BAs, and Ang II had no effect. Endothelial NO appears to be crucial in relaxation mediated with M3 and OK receptors but not β3-adrenergic receptors in the duck BA. The reduced endothelial NO involvement in the receptor-mediated relaxation response in duck BAs represents a clear difference from the corresponding response reported in chicken BAs. This physiological difference may explain the differences in lethality between ducks and chickens when vascular endothelial cells are infected with the virus.

    DOI: 10.3390/ani13172740

    Scopus

    PubMed

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MISC

  • 【マグネシウム代謝-その新たな臨床的意義】基礎と総論 マグネシウム濃度と生命予後との関連性 血圧と飲料水を中心に

    鈴木 舞, 新居 朋昭, 松岡 由紀, 澤 佐和子, 白石 光也, 宮本 篤

    腎と透析   86 ( 2 )   163 - 166   2019年2月

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    記述言語:日本語   出版者・発行元:(株)東京医学社  

  • Platelet dysfunction in Chediak-Higashi syndrome-affected cattle 査読

    Mitsuya Shiraishi, Hiroyuki Ogawa, Masahiro Ikeda, Sachiko Kawashima, Katsuaki Ito

    Journal of veterinary medical science   64 ( 9 )   751 - 760   2002年4月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

講演・口頭発表等

  • 俣野 拓友、植田 大海、Jannatul Ferdous、髙木 隆太、藤本 佳万、白石 光也、内藤 清惟 .  ラット脊髄腰仙髄部に投与したコレシストキニンが大腸運動を活性化する .  第167回日本獣医学会学術集会 

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    開催年月日: 2024年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:帯広市   国名:日本国  

  • Jannatul FERDOUS, Kiyotada NAITOU, Mitsuya SHIRAISHI .  Comparison of in vitro differentiation protocols for heavy metal-induced cytotoxicity in human neuroblastoma SH-SY5Y cells .  第51回日本毒性学会年会 

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    開催年月日: 2024年7月

    会議種別:ポスター発表  

  • 俣野 拓友、髙木 隆太、植田 大海、藤本 佳万、白石 光也、内藤 清惟 .  脊髄排便中枢におけるコレシストキニン8の作用の検討 .  第101回日本生理学会大会 

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    開催年月日: 2024年3月

  • 宮本篤、鈴木舞、呉思遠、大田和朋紀、スミスヘンリー、白石光也、松岡由紀、澤佐和子、森真理、森英樹、家森幸男 .  鹿児島県奄美群島における百寿者の地域分布と飲料水硬度の関係 .  第42回 日本マグネシウム学会学術集会 

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    開催年月日: 2023年10月

    会議種別:口頭発表(一般)  

  • Ferdous Jannatul、内藤 清惟、白石 光也 .  MARCKS-related peptide induced neurite outgrowth in SH-SY5Y cells .  第166回 日本獣医学会学術会議 

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    開催年月日: 2023年9月

    会議種別:口頭発表(一般)  

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