Updated on 2023/10/23

写真a

 
SHIRAISHI Mitsuya
 
Organization
Research Field in Veterinary Medicine, Agriculture, Fisheries and Veterinary Medicine Area Joint faculty of Veterinary Medicine Department of Veterinary Medicine Professor
Title
Professor
Contact information
メールアドレス

Degree

  • 博士（獣医学） ( 2001.3   山口大学 )

Research Interests

  • MARCKS protein

  • Intracellular signal transduction

  • Heavy metals

  • Platelet

  • Endothelial cell

  • Neuronal cell

Research Areas

  • Life Science / Veterinary medical science

Education

  • Yamaguchi University

    1997.4 - 2001.3

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    Country： Japan

  • University of Miyazaki

    1991.4 - 1997.3

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    Country： Japan

Research History

  • Kagoshima University   Research Field in Veterinary Medicine, Agriculture, Fisheries and Veterinary Medicine Area Joint faculty of Veterinary Medicine Department of Veterinary Medicine   Professor

    2019.2

  • Kagoshima University   Research Field in Veterinary Medicine, Agriculture, Fisheries and Veterinary Medicine Area, Joint faculty of Veterinary Medicine, Department of Veterinary Medicine   Associate Professor

    2012.4 - 2019.1

  • Kagoshima University   Associate Professor

    2007.4 - 2012.3

  • Kagoshima University   Associate Professor (as old post name)

    2007.3

  • Kitasato University   Research Assistant

    2004.4 - 2007.2

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    Country：Japan

  • University of Calgary   Smooth Muscle Research Group   Researcher

    2001.4 - 2004.3

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    Country：Canada

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Professional Memberships

  • 日本毒性学会

    2016.5

  • 日本マグネシウム学会

    2008.11

  • 日本薬理学会

    2007.3

  • 日本獣医学会

    2006.12

  • 日本生化学会

    2006.1

  • THE JAPANESE SOCIETY OF VETERINARY SCIENCE

  • THE JAPANESE SOCIETY OF TOXICOLOGY

  • The Japanese Society for Magnesium Research

  • THE JAPANESE BIOCHEMICAL SOCIETY

  • THE JAPANESE PHARMACOLOGICAL SOCIETY

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Papers

  • Siyuan Wu, Tomoki Ootawa, Ryoya Sekio, Henry Smith, Md Zahorul Islam, Ha Thi Thanh Nguyen, Yasuhiro Uno, Mitsuya Shiraishi, Atsushi Miyamoto .  Reduced Nitric Oxide Synthase Involvement in Aigamo Duck Basilar Arterial Relaxation .  Animals13 ( 17 )   2023.9Reviewed

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    The basilar arterial endothelium mediates blood vessel relaxation partly through the release of nitric oxide (NO). Apoptosis of cerebrovascular endothelial cells is linked to a high mortality rate in chickens infected with the highly pathogenic avian influenza virus, but interestingly, ducks exhibit a greater resistance to this virus. In this study, we examined the responsiveness of duck basilar arteries (BAs) to various vasoactive substances, including 5-hydroxytryptamine (5-HT), histamine (His), angiotensin (Ang) II, noradrenaline (NA), acetylcholine (ACh), and avian bradykinin ornithokinin (OK), aiming to characterize the receptor subtypes involved and the role of endothelial NO in vitro. Our findings suggest that arterial contraction is mediated with 5-HT1 and H1 receptors, while relaxation is induced with β3-adrenergic and M3 receptors. Additionally, OK elicited a biphasic response in duck BAs, and Ang II had no effect. Endothelial NO appears to be crucial in relaxation mediated with M3 and OK receptors but not β3-adrenergic receptors in the duck BA. The reduced endothelial NO involvement in the receptor-mediated relaxation response in duck BAs represents a clear difference from the corresponding response reported in chicken BAs. This physiological difference may explain the differences in lethality between ducks and chickens when vascular endothelial cells are infected with the virus.

    DOI： 10.3390/ani13172740

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  • Siyuan Wu, Tomoki Ootawa, Ryoya Sekio, Henry Smith, Md Zahorul Islam, Yasuhiro Uno, Mitsuya Shiraishi, Atsushi Miyamoto .  Involvement of beta3-adrenergic receptors in relaxation mediated by nitric oxide in chicken basilar artery. .  Poultry science102 ( 6 ) 102633 - 102633   2023.6Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    The response of basilar arteries to noradrenaline varies among many animal species, but remains little studied in poultry. Accordingly, we aimed to characterize the adrenergic receptor (AR) subtypes that modulate vascular response in basilar arteries in the chicken, with isometric recording of arterial ring tension using an organ bath. We demonstrated the presence of both alpha and beta (α and β) receptor subtypes through evaluating the response to noradrenaline, with and without a range of β-AR and α-AR antagonists. The concentration-dependent relaxations then induced by a range of β-AR agonists indicated a potency ranking of isoproterenol > noradrenaline > adrenaline > procaterol. We then investigated the effects of β-AR antagonists that attenuate the effect of isoproterenol (propranolol for β1,2,3-ARs, atenolol for β1-ARs, butoxamine for β2-ARs, and SR 59230A for β3-ARs), with Schild regression analysis, ascertaining multiple β-AR subtypes, with neither the β1-AR nor the β2-AR as the dominant subtype. SR 59230A was the only antagonist to yield a pA2 value (7.52) close to the reported equivalent for the relevant receptor subtype. Furthermore, treatment with SR 58611 (a β3-AR agonist) induced relaxation, which was inhibited (P < 0.01) by L-NNA and SR 59230A. Additionally, treating basilar arterial strips (containing endothelium) with SR 58611 induced nitric oxide (NO) production, which was inhibited (P < 0.01) by L-NNA and SR 59230A. Based on this first characterization of AR subtypes in chicken basilar arteries (to our knowledge), we suggest that α- and β-ARs are involved in contraction and relaxation, and that β3-ARs, especially those on the endothelium, may play an important role in vasodilation via NO release.

    DOI： 10.1016/j.psj.2023.102633

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  • Yasuhiro Uno, Saho Morikuni, Mitsuya Shiraishi, Atsushi Asano, Norie Murayama, Hiroshi Yamazaki .  Novel Cytochrome P450 2C94 Functionally Metabolizes Diclofenac and Omeprazole in Dogs. .  Drug metabolism and disposition: the biological fate of chemicals51 ( 5 ) 637 - 644   2023.5Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    Cytochromes P450 (P450s or CYPs) are important drug-metabolizing enzymes. Because dogs are frequently used in drug metabolism studies, knowledge of dog CYP2C enzymes is essential because in humans these enzymes are abundant and play major roles in liver and intestine. The present study identified and characterized novel dog CYP2C94 along with previously identified dog CYP2C21 and CYP2C41. Dog CYP2C21, CYP2C41, and CYP2C94 cDNAs, respectively, contained open reading frames of 490, 489, and 496 amino acids and shared high-sequence identities (70%, 75%, and 58%) with human CYP2Cs. Dog CYP2C94 mRNA was preferentially expressed in liver, just as dog CYP2C21 and CYP2C41 mRNAs were. In dog liver, CYP2C21 mRNA was the most abundant, followed by CYP2C94 and CYP2C41 mRNAs. Moreover, the hepatic expressions of all three dog CYP2C mRNAs varied in four individual dogs, two of which did not express CYP2C41 mRNA. The three dog CYP2C genes had similar gene structures, and CYP2C94, although located on the same chromosome, was in a genomic region far from the gene cluster containing CYP2C21 and CYP2C41 Metabolic assays with recombinant proteins showed that dog CYP2C94, along with CYP2C21 and CYP2C41, efficiently catalyzed oxidations of diclofenac, warfarin, and/or omeprazole, indicating that dog CYP2C94 is a functional enzyme. Novel dog CYP2C94 is expressed abundantly in liver and encodes a functional enzyme that metabolizes human CYP2C substrates; it is, therefore, likely responsible for drug clearances in dogs. SIGNIFICANCE STATEMENT: Novel dog cytochrome P450 2C94 (CYP2C94) was identified and characterized along with dog CYP2C21 and CYP2C41. Dog CYP2C94, isolated from liver, had 58% sequence identity and a close phylogenetic relationship with its human homologs and was expressed in liver at the mRNA level. Dog CYP2C94 (and CYP2C21 and CYP2C41) catalyzed oxidations of diclofenac and omeprazole, human CYP2C9 and CYP2C19 substrates, respectively, but CYP2C41 also hydroxylated warfarin. CYP2C94 is therefore a functional drug-metabolizing enzyme likely responsible for drug clearances in dogs.

    DOI： 10.1124/dmd.122.001236

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  • Mai Suzuki, Siyuan Wu, Tomoki Ootawa, Henry Smith, Mitsuya Shiraishi, Atsushi Miyamoto, Yuki Matsuoka, Sawako Sawa, Mari Mori, Hideki Mori, Yukio Yamori .  Relationship between Regional Distribution of Centenarians and Drinking Water Hardness in the Amami Islands, Kagoshima Prefecture, Japan. .  Nutrients15 ( 7 )   2023.3Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    People who drink naturally hardened water may experience longevity-enhancing effects. In this study, we investigated water hardness and longevity from both geological and epidemiological perspectives in Japan's Amami islands, where drinking water is drawn from coralline or non-coralline bedrock. We investigated drinking water hardness, limestone bedrock occupancy, and the centenarian rate (number per 10,000 population) by municipality across four adjacent islands (Amami-Oshima (non-coralline), Tokunoshima, Okinoerabu, and Yoron (predominantly coralline)). Limestone was strongly correlated with water hardness (r = 0.99; p < 0.01), occupying more than 80% of the bedrock where the water was the hardest (Tokunoshima's Isen municipality: 86.5%; Yoron: 82.9%) and being scarcely detectable in Amami-Oshima (0.0 to 0.2%), where the water was the least hard. The centenarian rate was also strongly correlated with water hardness (r = 0.84, p < 0.01), with the highest figures in Yoron (29.7) and Isen (29.2), and the lowest in Amami-Oshima (0.0 to 12.2). Therefore, we hypothesize a potentially beneficial effect of hard water on longevity when that water is drawn from coralline limestone. Water hardness is determined by the water content of calcium and magnesium and may plausibly influence life expectancy through a preventative effect against cardiovascular disease. Our findings are of interest to current debates about future global access to drinking water and its quality.

    DOI： 10.3390/nu15071569

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  • Yasuhiro Uno, Saho Morikuni, Mitsuya Shiraishi, Atsushi Asano, Hiroaki Kawaguchi, Norie Murayama, Hiroshi Yamazaki .  A comprehensive analysis of six forms of cytochrome P450 2C (CYP2C) in pigs. .  Xenobiotica; the fate of foreign compounds in biological systems52 ( 9-11 ) 1 - 26   2022.11Reviewed International journal

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    Pigs are an important species used in drug metabolism studies; however, the cytochromes P450 (P450s or CYPs) have not been fully investigated in pigs.In this study, pig CYP2C32, CYP2C33, CYP2C34, CYP2C36, CYP2C42, and CYP2C49 cDNAs were isolated and found to contain open reading frames of 490 or 494 amino acids that shared 64-82% sequence identity with human CYP2C8/9/18/19.Pig CYP2C genes formed a gene cluster in a genomic region that corresponded to that of the human CYP2C cluster; an additional gene cluster was formed by pig CYP2C33a and CYP2C33b distant from the first cluster but located in the same chromosome.Among the tissues analysed, these pig CYP2C mRNAs were preferentially expressed in liver, small intestine, and/or kidney; pig CYP2C49, CYP2C32, CYP2C34, and CYP2C33 mRNAs were the most abundant CYP2C mRNAs in liver, jejunum, ileum, and kidney, respectively.Metabolic assays showed that pig CYP2C proteins (heterologously expressed in Escherichia coli) metabolised typical human CYP2C substrates diclofenac, warfarin, and/or omeprazole.The results suggest that these pig CYP2Cs are functional enzymes able to metabolise human CYP2C substrates in liver and small intestine, just as human CYP2Cs do.

    DOI： 10.1080/00498254.2022.2148139

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  • Yasuhiro Uno, Shotaro Uehara, Moe Ijiri, Hiroaki Kawaguchi, Atsushi Asano, Mitsuya Shiraishi, Kaito Banju, Norie Murayama, Hiroshi Yamazaki .  Molecular and Functional Characterization of N-Acetyltransferases in Common Marmosets and Pigs. .  Drug metabolism and disposition: the biological fate of chemicals50 ( 11 ) 1429 - 1433   2022.11Reviewed International journal

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Arylamine N-acetyltransferases (NATs) are drug-metabolizing enzymes that are essential for the metabolism of endogenous substrates and xenobiotics. The molecular characteristics of NATs have been extensively investigated in humans but remain to be investigated in common marmosets and pigs, animal species that are often used in drug metabolism studies. In this study, marmoset NAT1 and pig NAT1 cDNAs were isolated from liver samples and were characterized by molecular analyses and drug-metabolism assays. These NAT genes were intronless and formed gene clusters with one other NAT gene in the genome, just as human NAT genes do. Marmoset NAT1 and pig NAT1 amino acid sequences showed high sequence identities (94% and 85%, respectively) to human NAT1. Phylogenetic analysis indicated that marmoset NAT1 and pig NAT1 were more closely clustered with human NATs than with rat or mouse NATs. Marmoset NAT1 and pig NAT1 mRNAs were expressed in all the tissue types analyzed, with the expression levels being highest in the small intestine. Metabolic assays using recombinant proteins found that marmoset NAT1 and pig NAT1 metabolized human NAT substrates p-aminobenzoic acid, 2-aminofluorene, sulfamethazine, and isoniazid. Marmoset NAT1 and pig NAT1 substantially acetylated p-aminobenzoic acid and 2-aminofluorene relevant human NAT1, but their activities were lower toward sulfamethazine and isoniazid than those of the relevant human NAT2. Therefore, marmoset and pig NATs are functional enzymes with molecular similarities to human NAT1, but their substrate specificities, while similar to human NAT1, differ somewhat from human NAT2. SIGNIFICANCE STATEMENT: Marmoset N-acetyltransferase NAT1 and pig NAT1 were identified and showed high sequence identities to human NAT1. These NAT mRNAs were expressed in various tissues. Marmoset and pig NAT1s acetylated typical human NAT substrates, although their substrate specificities differed somewhat from human NAT2. Marmoset NAT1 and pig NAT1 have similarities with human NAT1 in terms of molecular and enzymatic characteristics.

    DOI： 10.1124/dmd.122.000919

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  • Yasuhiro Uno, Norie Murayama, Moe Ijiri, Hiroaki Kawaguchi, Osamu Yamato, Mitsuya Shiraishi, Atsushi Asano, Hiroki Teraoka, Hazuki Mizukawa, Shouta M M Nakayama, Yoshinori Ikenaka, Mayumi Ishizuka, Hiroshi Yamazaki .  Cytochrome P450 2J Genes Are Expressed in Dogs, Cats, and Pigs, and Encode Functional Drug-Metabolizing Enzymes. .  Drug metabolism and disposition: the biological fate of chemicals50 ( 11 ) 1434 - 1441   2022.11Reviewed International journal

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Cytochrome P450s (P450s) have been identified and analyzed in dogs and pigs, species that are often used in preclinical drug studies. Moreover, P450s are clinically important for drug therapy not only in humans, but also in species under veterinary care, including dogs and cats. In the present study, seven P450s homologous to human CYP2J2, namely, dog CYP2J2; cat CYP2J2; and pig CYP2J33, CYP2J35, CYP2J91, and CYP2J93, were newly identified and characterized, along with pig CYP2J34 previously identified. The cDNAs of these CYP2Js contain open reading frames of 502 amino acids, except for CYP2J35 (498 amino acids), and share high sequence identity (77%-80%) with human CYP2J2. Phylogenetic analysis revealed that dog and cat CYP2J2 were closely related, whereas pig CYP2Js formed a cluster. All seven CYP2J genes contain nine coding exons and are located in corresponding genomic regions, with the pig CYP2J genes forming a gene cluster. These CYP2J2 mRNAs were predominantly expressed in the small intestine with additional expression in the kidney and brain for dog CYP2J2 and pig CYP2J91 mRNAs, respectively. All seven CYP2Js metabolized human CYP2J2 substrates terfenadine, ebastine, and astemizole, indicating that they are functional enzymes. Dog CYP2J2 and pig CYP2J34 and CYP2J35 efficiently catalyzed ebastine primary hydroxylation and secondary carebastine formation at low substrate concentrations, just as human CYP2J2 does. Velocity-versus-substate plots exhibited sigmoidal relationships for dog CYP2J2, cat CYP2J2, and pig CYP2J33, indicating allosteric interactions. These results suggest that dog, cat, and pig CYP2Js have similar functional characteristics to human CYP2J2, with slight differences in ebastine and astemizole oxidations. SIGNIFICANCE STATEMENT: Dog CYP2J2; cat CYP2J2; and pig CYP2J33, CYP2J34, CYP2J35, CYP2J91, and CYP2J93, homologous to human CYP2J2, were identified and characterized by sequence, phylogenetic, and genomic structure analyses. Intestinal expression patterns of CYP2J mRNAs were characteristic in dogs, cats, and pigs. Dog, cat, and pig CYP2Js likely play roles as drug-metabolizing enzymes in the small intestine, similar to human CYP2J2.

    DOI： 10.1124/dmd.122.000930

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  • NII Tomoaki, ISLAM Md Zahorul, KAKE Satoru, SHIRAISHI Mitsuya, TAKEUCHI Takashi, KUWATA Hidefumi, MIYAMOTO Atsushi, HARADA Etsumori .  Direct evidence of nitric oxide production induced by lactoferrin and its enhancement by magnesium ions in cultured endothelial cells .  Journal of Veterinary Medical Science84 ( 11 ) 1499 - 1501   2022.9Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE  

    <p>Bovine lactoferrin (BLF) reportedly lowers blood pressure and induces vasorelaxation, but its effect on nitric oxide (NO) production has not been established. Accordingly, we aimed to determine whether BLF induces NO production in bovine aortic endothelial cells, and the effects of extracellular free magnesium (Mg) ion concentrations on this NO production. BLF induced NO production time-dependently. NO production was markedly inhibited by the NO synthase inhibitor, <i>N<sup>G</sup></i>-nitro-L-arginine methyl ester, in an effect abolished by L-arginine, but not D-arginine. NO production was suppressed at low concentrations, and enhanced at high concentrations, of Mg ions in culture medium. These results suggest that BLF has an important role in hypotensive effects. Mg ions may affect BLF-induced NO production.</p>

    DOI： 10.1292/jvms.22-0368

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  • Kiyotada Naitou, Honoka Iwashita, Hiromi H Ueda, Mitsuya Shiraishi, Yoshikazu Fujimoto, Kazuhiro Horii, Tomoya Sawamura, Takahiko Shiina, Yasutake Shimizu .  Intrathecally administered substance P activated the spinal defecation center and enhanced colorectal motility in anesthetized rats. .  American journal of physiology. Gastrointestinal and liver physiology323 ( 1 ) G21-G30 - G30   2022.4Reviewed International journal

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    Noxious stimuli on the colorectum cause colorectal contractions through activation of descending monoaminergic pathways projecting from the supraspinal defecation center to the spinal defecation center. Since it is known that substance P is involved in the response to peripheral noxious stimuli in the spinal cord, we investigated the effects of intrathecally administered substance P at L6-S1 levels on colorectal motility in rats that were anesthetized with alpha-chloralose and ketamine. Intrathecally administered substance P enhanced colorectal motility, even after transection of the thoracic spinal cord at the T4 level. Severing the pelvic nerves, but not the colonic nerves, abolished substance P-enhanced colorectal motility. In the spinal cord at L6-S1 levels, expression of mRNA coding NK1-3 receptors was detected by RT-PCR. Immunohistochemical experiments revealed that preganglionic neurons of the pelvic nerves express NK1 receptors, while expression of NK2 receptors was not found. In addition, substance P-containing fibers densely innervated around the preganglionic neurons expressing NK1 receptors. An intrathecally administered NK1 receptor antagonist (spantide) attenuated capsaicin-induced colorectal contractions. These results suggest that the colokinetic action of substance P is mediated by the NK1 receptor in the spinal defecation center. Our findings indicate that substance P may function as a neurotransmitter in the spinal defecation center.

    DOI： 10.1152/ajpgi.00342.2021

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  • T. Ozawa, N. Miura, H. Hasegawa, T. Uemura, Y. Nakamoto, M. Tsujio, T. Takeuchi, M. Shiraishi .  Characteristics and outcome of suspected cerebrovascular disease in dogs: 66 cases (2009‐2016) .  Journal of Small Animal Practice63 ( 1 ) 45 - 51   2022.1Reviewed

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Objectives : To characterise the clinical signs of suspected cerebrovascular disease in dogs. Materials and Methods : Medical records of one hospital were searched from November 2009 to December 2016 for dogs that suffered of cerebrovascular disease. We diagnosed cerebrovascular disease based on acute onset, clinical signs and magnetic resonance imaging findings. The medical history, clinical signs, concurrent disease, area of infarction, cerebrospinal fluid results, month at onset and outcome were investigated in the cerebrovascular disease group and in a control group (dogs with brain disorders other than cerebrovascular disease). Results : A total of 122 CVD cases were extracted from the 5312 patients that visited during the study period. Of these 122 cases, 66 (1.2%) matched the subject selection criteria of our study and were included in the analysis. Forebrain infarction was observed in 51 of 66 cases, of which 24 (47.1%) suffered from seizures. The number of dogs diagnosed with cerebrovascular disease was disproportionately high in August (nine of 59 cases) and December (13 of 59 cases). In the outcome survey, deterioration was observed in 11 of 55 cases. Clinical Significance : Seizure is an important clinical sign of cerebrovascular disease in dogs. There was a significant seasonal variation in the number of dogs diagnosed with cerebrovascular disease in Japan. Clinical features observed in this report differ from those of previous reports and highlight the need for additional research in this area.

    DOI： 10.1111/jsap.13422

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  • Md Zahorul Islam, Shusuke Kojima, Masamichi Sameshima, Takeshi Obi, Emi Yamazaki-Himeno, Mitsuya Shiraishi, Atsushi Miyamoto .  Vasomotor effects of noradrenaline, 5-hydroxytryptamine, angiotensin II, bradykinin, histamine, and acetylcholine on the bat (Rhinolophus ferrumequinum) basilar artery .  Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology250   109190 - 109190   2021.12Reviewed International coauthorship International journal

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    The responsiveness of the basilar artery to intrinsic vasoactive substances is species-specific and can be a unique characteristic. We investigated the responsiveness of the bat (Rhinolophus ferrumequinum) basilar artery to noradrenaline (NA), 5-hydroxytryptamine (5-HT), angiotensin (Ang) II, bradykinin (BK), histamine (His), and acetylcholine (ACh). NA, 5-HT, Ang II, and BK induced contraction, whereas His and ACh induced relaxation, in a concentration-dependent manner. The NA cumulative concentration-response curve was shifted to the right in parallel with phentolamine (an α-antagonist). However, propranolol, a β-antagonist, had no significant effect. The 5-HT curve was shifted to the right in parallel by ketanserin (a 5-HT2 antagonist) and methiothepin (a 5-HT1 and 5-HT2 antagonist). Losartan (an AT1 antagonist) shifted the Ang II curve to the right, whereas PD123319 (an AT2 antagonist) had no significant effect. L-NA, indomethacin, and des-Arg9-[Leu8]-BK (a B1 antagonist) did not significantly affect BK-induced contractions. HOE140 (a B2 antagonist) shifted the BK concentration-response curve to the right. The His curve was shifted to the right weakly by diphenhydramine (an H1 antagonist) and strongly by cimetidine (a H2 antagonist). ACh-induced relaxation was significantly inhibited by L-NA, atropine, and pFHHSiD (a muscarinic M3 antagonist), whereas pirenzepine and methoctramine (muscarinic M1 and M2 antagonists, respectively) showed no significant effects. At a resting vascular tone, L-NA-induced contraction and indomethacin induced relaxation. These results suggest that α-adrenergic, 5-HT1, 5-HT2, AT1, and B2 receptors might be important in arterial contraction, whereas M3 and H2 (>H1) receptors might modify these contractions, inducing relaxation.

    DOI： 10.1016/j.cbpc.2021.109190

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  • OZAWA Tsuyoshi, IJICHI Takashi, SHIRAISHI Mitsuya .  Measurement of canine blood microparticles by flow cytometry: effect of anticoagulants and staining reagents .  Journal of Veterinary Medical Science83 ( 11 ) 1786 - 1789   2021.11Reviewed

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE  

    <p>Microparticles (MPs) are released from budding plasma membranes into body fluids. The use of flow cytometry for the measurement of MP in canines has not been standardized. In this fundamental study, we compared the effect of anticoagulant agents, such as acid-citrate-dextrose (ACD) and heparin on the measurement of canine MPs in platelet-free plasma (PFP) using flow cytometry. In addition, we used annexin V, carboxyfluorescein succinimidyl ester (CFSE), or calcein tetraacetoxymethyl ester (calcein-AM), and explored the characteristics of the staining reagents in MP detection using flow cytometry. We were able to measure canine MPs in PFP prepared from ACD-anticoagulated blood using flow cytometry, in which the highest positive rate for fluorescent staining was observed when CFSE was used.</p>

    DOI： 10.1292/jvms.21-0448

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  • Hiromi H Ueda, Kiyotada Naitou, Hiroyuki Nakamori, Kazuhiro Horii, Takahiko Shiina, Tatsunori Masatani, Mitsuya Shiraishi, Yasutake Shimizu .  α-MSH-induced activation of spinal MC1R but not MC4R enhances colorectal motility in anaesthetised rats .  Scientific reports11 ( 1 ) 487 - 487   2021.1Reviewed International journal

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    The central nervous system is involved in regulation of defaecation. It is generally considered that supraspinal regions control the spinal defaecation centre. However, signal transmission from supraspinal regions to the spinal defaecation centre is still unclear. In this study, we investigated the regulatory role of an anorexigenic neuropeptide, α-MSH, in the spinal defaecation centre in rats. Intrathecal administration of α-MSH to the L6-S1 spinal cord enhanced colorectal motility. The prokinetic effect of α-MSH was abolished by severing the pelvic nerves. In contrast, severing the colonic nerves or thoracic cord transection at the T4 level had no impact on the effect of α-MSH. RT-PCR analysis revealed MC1R mRNA and MC4R mRNA expression in the L6-S1 spinal cord. Intrathecally administered MC1R agonists, BMS470539 and SHU9119, mimicked the α-MSH effect, but a MC4R agonist, THIQ, had no effect. These results demonstrate that α-MSH binds to MC1R in the spinal defaecation centre and activates pelvic nerves, leading to enhancement of colorectal motility. This is, to our knowledge, the first report showing the functional role of α-MSH in the spinal cord. In conclusion, our findings suggest that α-MSH is a candidate for a neurotransmitter from supraspinal regions to the spinal defaecation centre.

    DOI： 10.1038/s41598-020-80020-x

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  • Md Zahorul Islam, Yuji Sawatari, Shusuke Kojima, Yusuke Kiyama, Moe Nakamura, Kyouko Sasaki, Mika Otsuka, Takeshi Obi, Mitsuya Shiraishi, Atsushi Miyamoto .  Vasomotor effects of 5-hydroxytryptamine, histamine, angiotensin II, acetylcholine, noradrenaline, and bradykinin on the cerebral artery of bottlenose dolphin (Tursiops truncatus). .  The Journal of veterinary medical science82 ( 10 ) 1456 - 1463   2020.10Reviewed

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    From an evolutionary aspect, dolphins share a very close phylogenetic relationship with pigs. Previously, we characterized porcine cerebral artery responsiveness to intrinsic vasoactive substances. Therefore, here, we investigated dolphin (Tursiops truncatus) cerebral artery responsiveness to 5-hydroxytryptamine (5-HT), histamine (His), angiotensin (Ang) II, acetylcholine (ACh), noradrenaline (NA), and bradykinin (BK) to characterize their related receptor subtypes. We also compared dolphin cerebral artery responsiveness with porcine cerebral artery responsiveness. We found that 5-HT and His induced concentration-dependent contraction of the dolphin cerebral artery. Ketanserin (a 5-HT2 antagonist) and methiothepin (a 5-HT1 and 5-HT2 antagonist) shifted the concentration-response curve for 5-HT to the right. Although diphenhydramine (an H1 antagonist) shifted the concentration-response curve for His to the right, cimetidine (an H2 antagonist) had no such effect. Ang II and ACh did not produce any vasomotor actions. NA induced concentration-dependent relaxation. Propranolol (a β antagonist) shifted the concentration-response curve for NA to the right, whereas phentolamine (an α antagonist) had no significant effect. BK induced relaxation followed by contraction in pre-contracted arteries with intact endothelium. HOE140 (a B2 antagonist) shifted the concentration-response curve for BK to the right, whereas des-Arg9-[Leu8]-BK (a B1 antagonist) had no significant effect. These results suggest that 5-HT1, 5-HT2, and H1 receptor subtypes are important in arterial contraction and that β and B2 receptor subtypes modify these contractions to relaxations. The responsiveness of the dolphin cerebral artery is very similar to that of porcine cerebral artery, supporting their evolutionary linkage.

    DOI： 10.1292/jvms.20-0351

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  • Kasumi Sudo, Cuong VAN Dao, Atsushi Miyamoto, Mitsuya Shiraishi .  Comparative analysis of in vitro neurotoxicity of methylmercury, mercury, cadmium, and hydrogen peroxide on SH-SY5Y cells. .  The Journal of veterinary medical science81 ( 6 ) 828 - 837   2019.6Reviewed

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Mercury (Hg) and cadmium (Cd) are the major toxic heavy metals and are known to induce neurotoxicity. Although many studies have shown that several heavy metals have neurotoxic effects, the cellular and molecular mechanisms thereof are still not clear. Oxidative stress is reported to be a common and important mechanism in cytotoxicity induced by heavy metals. However, the assays for identifying toxic mechanisms were not performed under the same experimental conditions, making it difficult to compare toxic properties of the heavy metals. In this study, we investigated the mechanisms underlying neurotoxicity induced by heavy metals and H2O2, focusing on cell death, cell proliferation, and oxidative stress under the same experimental condition. Our results showed that MeHg caused lactate dehydrogenase (LDH) release, caspase activation and cell-cycle alteration, and ROS generation in accordance with decreased cell viability. HgCl2 caused LDH release and cell-cycle alteration, but not caspase activation. CdCl2 had a remarkable effect on the cell cycle profiles without induction of LDH release, caspase activation, or ROS generation. Pretreatment with N-acetyl-l-cysteine (NAC) prevented the decrease in cell viability induced by MeHg and HgCl2, but not CdCl2. Our results demonstrate a clear difference in neurotoxic mechanisms induced by MeHg, HgCl2, CdCl2 or H2O2 in SH-SY5Y cells. Elucidating the characteristics and mechanisms of each heavy metal under the same experimental conditions will be helpful to understand the effect of heavy metals on health and to develop a more effective therapy for heavy metal poisoning.

    DOI： 10.1292/jvms.19-0059

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  • Cuong Van DAO, Mitsuya SHIRAISHI, Atsushi MIYAMOTO .  The MARCKS protein amount is differently regulated by calpain during toxic effects of methylmercury between SH-SY5Y and EA.hy926 cells. .  Journal of Veterinary Medical Science79 ( 12 ) 1931 - 1938   2017.12The MARCKS protein amount is differently regulated by calpain during toxic effects of methylmercury between SH-SY5Y and EA.hy926 cells.Reviewed

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    DOI： https://doi.org/10.1292/jvms.17-0473

  • Md Zahorul Islam, Hiroaki Kawaguchi, Naoki Miura, Noriaki Miyoshi, Emi Yamazaki-Himeno, Mitsuya Shiraishi, Atsushi Miyamoto, Akihide Tanimoto .  Hypertension alters the endothelial-dependent biphasic response of bradykinin in isolated Microminipig basilar artery. .  Microvascular research114   52 - 57   2017.11Reviewed International journal

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    Angiotensin (Ang) II is known to promote vascular disease and hypertension, partly through its effect on vascular endothelium. Bradykinin (BK) is an endothelium-dependent agonist that induces relaxation followed by contraction of the porcine basilar artery through release of NO and PGF2α, respectively. In this study, we evaluated the effect of Ang II-induced hypertension on basilar artery responsiveness to BK in the Microminipig (MMPig). Ang II (200ng/kg/min) or vehicle was infused into MMPigs for 14days using an osmotic mini-pump and blood pressure was monitored regularly. The responsiveness of subsequently isolated basilar arteries was then measured using a micro organ bath system. MMPig basilar artery endothelial cells were cultured and stimulated with Ang II or vehicle for 48h. Mean blood pressure was significantly (P<0.05; n=5) higher in Ang II-infused MMPigs than in vehicle-infused MMPigs. In vitro, BK-induced endothelium-dependent dilation of isolated basilar artery specimens was abolished and BK-induced contraction was significantly increased (Emax: 15.85±2.42% and 56.54±2.71% of 60mM KCl in control and Ang II group respectively at 10-7M concentration of BK; P<0.01; n=5) in Ang II-infused MMPigs. Ang II stimulation of the endothelial cells significantly decreased (54.15% at 24h; P<0.05; n=three independent experiment performed in triplicate) the amount of BK-elicited NO and increased (44.27% at 24h; P<0.05; n=three independent experiment performed in triplicate) the amount of BK-elicited PGF2α. These results suggest that the decrease of NO and increase of PGF2α production from endothelial cells are responsible for cerebrovascular dysfunction in hypertension, possibly causing cerebrovascular contraction and thus increasing the risk of brain infarction.

    DOI： 10.1016/j.mvr.2017.06.001

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  • Md Zahorul Islam, Cuong Van Dao, Atsushi Miyamoto, Mitsuya Shiraishi .  Rho-kinase and the nitric oxide pathway modulate basilar arterial reactivity to acetylcholine and angiotensin II in streptozotocin-induced diabetic mice. .  Naunyn-Schmiedeberg's archives of pharmacology390 ( 9 ) 929 - 938   2017.9Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER  

    Diabetes mellitus comprises a heterogeneous group of metabolic disorders with underlying hyperglycemia and secondary cardiovascular complications. Growing evidence suggests that vascular dysfunction is among the most important causes of diabetic cardiovascular disease. Therefore, we determined whether streptozotocin (STZ)-induced diabetes in mice affects blood pressure and cerebral arterial responsiveness to angiotensin (Ang) II and acetylcholine (ACh), which are important modulators of cerebrovascular autoregulation. Diabetes was induced using a single intraperitoneal injection of STZ (50 mg/kg). Blood pressure was measured in conscious mice using the indirect tail-cuff method. Functional studies of the isolated arteries' response to vasoactive substances were performed using a micro-organ-bath system at 60 days after STZ injection. Systolic, diastolic, and mean blood pressures significantly increased at days 45 and 60 in the STZ-induced diabetic mice. In the isolated basilar arteries, ACh-induced relaxation, which is dependent on nitric oxide (NO) production from endothelial cells, decreased. In contrast, Ang II-induced contraction, mediated via rho-kinase activation in the smooth muscle, increased in the diabetic mice. There was significantly greater relaxation in the precontracted isolated basilar arteries of diabetic mice that had been treated with Y27632, a rho-kinase inhibitor, than in the control mice arteries. Pretreatment with Nω-nitro-L-arginine (L-NAME), an NO synthase inhibitor, significantly enhanced Ang II-induced contraction and Y27632-induced relaxation in the control basilar arteries but not in the STZ-induced diabetic mice arteries. These results suggest that decreased NO bioavailability and enhanced rho-kinase activity in basilar arteries contribute to altered reactivity to ACh and Ang II, respectively, in STZ-induced diabetic mice.

    DOI： 10.1007/s00210-017-1396-x

    Web of Science

    Scopus

    PubMed

  • Cuong Van Dao, Md. Zahorul Islam, Kasumi Sudo, Mitsuya Shiraishi, Atsushi Miyamoto .  MARCKS is involved in methylmercury-induced decrease in cell viability and nitric oxide production in EA.hy926 cells. .  Journal of veterinary medical science78 ( 10 ) 1569 - 1576   2016.11MARCKS is involved in methylmercury-induced decrease in cell viability and nitric oxide production in EA.hy926 cells.Reviewed

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    DOI： 10.1292/jvms.16-0249

  • Ha Thi Thanh Nguyen, Hai Thanh Nguyen, Md. Zahorul Islam, Takeshi Obi, Pitchaya Pothinuch, Thanh Van Nguyen, Tuong Manh Nguyen, Cuong Van Dao, Mitsuya Shiraishi, Atsushi Miyamoto .  Antagonistic effects of Gingko biloba and Sophora japonica on cerebral vasoconstriction in response to histamine, 5-hydroxytryptamine, U46619 and bradykinin .  American journal of chinese medicine44 ( 8 ) 1607 - 1625   2016.11Antagonistic effects of Gingko biloba and Sophora japonica on cerebral vasoconstriction in response to histamine, 5-hydroxytryptamine, U46619 and bradykininReviewed

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    DOI： 10.1142/S0192415X16500907

  • Masato Ohkubo, Atsushi Miyamoto, Mitsuya Shiraishi .  Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury. .  Journal of veterinary medical science78 ( 5 ) 761 - 767   2016.6Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury.Reviewed

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    DOI： 10.1292/jvms.15-0620

  • Ha Thi Thanh Nguyen, Hai Thanh Nguyen, Md. Zahorul Islam, Takeshi Obi, Pitchaya Pothinuch, Phyu Phyu Khine Zar, De Xing Hou, Thanh Van Nguyen, Tuong Manh Nguyen, Cuong Van Dao, Mitsuya Shiraishi, Atsushi Miyamoto .  Pharmacological characteristics of Artemisia vulgaris L. in isolated porcine basilar artery. .  Journal of ethnopharmacology182   16 - 26   2016.4Pharmacological characteristics of Artemisia vulgaris L. in isolated porcine basilar artery.Reviewed

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    DOI： 10.1016/j.jep.2016.02.009

  • Md. Zahorul Islam, Cuong Van Dao, Mitsuya Shiraishi, Atsushi Miyamoto .  Methylmercury affects cerebrovascular reactivity to angiotensin II and acetylcholine via Rho-kinase and nitric oxide pathways in mice .  Life sciences147   30 - 38   2016.2Methylmercury affects cerebrovascular reactivity to angiotensin II and acetylcholine via Rho-kinase and nitric oxide pathways in miceReviewed

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    DOI： 10.1016/j.lfs.2016.01.033

  • Akihiko Hatano, Mitsuya Shiraishi, Nanae Terado, Atsuhiro Tanabe, Kenji Fukuda .  Enzymatic synthesis and RNA interference of nucleosides incorporating stable isotopes into a base moiety .  Bioorganic & medicinal chemistry23 ( 20 ) 6683 - 6688   2015.10Enzymatic synthesis and RNA interference of nucleosides incorporating stable isotopes into a base moietyReviewed

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    DOI： 10.1016/j.bmc.2015.09.011

  • Atsuhiro Tanabe, Mitsuya Shiraishi, Yasuharu Sasaki .  Myristoylated alanine-rich C kinase substrate accelerates TNF-α-induced apoptosis in SH-SY5Y cells in a caspases-6 and/or -7-dependent manner .  Advances in bioscience and biotechnology6 ( 8 ) 572 - 582   2015.8Myristoylated alanine-rich C kinase substrate accelerates TNF-α-induced apoptosis in SH-SY5Y cells in a caspases-6 and/or -7-dependent mannerReviewed

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    DOI： 10.4236/abb.2015.68060

  • Md. Zahorul Islam, Yutaka Watanabe, Ha Thi Thanh Nguyen, Emi Yamazaki-Himeno, Takeshi Obi, Mitsuya Shiraishi, Atsushi Miyamoto .  Vasomotor effects of acetylcholine, bradykinin, noradrenaline, 5-hydroxytryptamine, histamine and angiotensin II on the mouse basilar artery .  Journal of veterinary medical science76 ( 10 ) 1339 - 1345   2014.10Vasomotor effects of acetylcholine, bradykinin, noradrenaline, 5-hydroxytryptamine, histamine and angiotensin II on the mouse basilar arteryReviewed

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  • Md. Zahorul Islam, Kaori Miyagi, Tsukasa Matsumoto, Ha Thi Thanh Nguyen, Emi Yamazaki-Himeno, Mitsuya Shiraishi, Atsushi Miyamoto. .  Bradykinin induces NO and PGF2α production via B2 receptor activation from cultured porcine basilar arterial endothelial cells. .  Naunyn-Schmiedeberg's archives of pharmacology387 ( 7 ) 697 - 702   2014.7Bradykinin induces NO and PGF2α production via B2 receptor activation from cultured porcine basilar arterial endothelial cells.Reviewed

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  • Mitsuya Shiraishi, Makoto Hangai, Megumi Yamamoto, Masanori Sasaki, Atsuhiro Tanabe, Yasuharu Sasaki, Atsushi Miyamoto .  Alteration in MARCKS phosphorylation and expression by methylmercury in SH-SY5Y cells and rat brain .  Environmental toxicology and pharmacology37 ( 3 ) 1256 - 1263   2014.3Alteration in MARCKS phosphorylation and expression by methylmercury in SH-SY5Y cells and rat brainReviewed

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  • Atsuhiro Tanabe, Mitsuya Shiraishi, Yasuharu Sasaki .  Dephosphorylation of myristoylated alanine-rich C kinase substrate accelerates wound-induced migration of SH-SY5Y cells .  Advances in bioscience and biotechnology4 ( 8B ) 27 - 32   2013.8Dephosphorylation of myristoylated alanine-rich C kinase substrate accelerates wound-induced migration of SH-SY5Y cellsReviewed

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  • Mitsuya Shiraishi, Kazuya Tamura, Mina Egoshi, Atsushi Miyamoto .  Cholesterol enrichment of rabbit platelets enhances the Ca(2+) entry pathway induced by platelet-derived secondary feedback agonists .  Life sciences92 ( 14-16 ) 838 - 844   2013.5Cholesterol enrichment of rabbit platelets enhances the Ca(2+) entry pathway induced by platelet-derived secondary feedback agonistsReviewed

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  • Megumi Yamamoto, Motohiro Takeya, Hiroko Ikeshima-Kataoka, Masato Yasui, Yasuaki Kawasaki, Mitsuya Shiraishi, Eiji Majima, Seiji Shiraishi, Yasuhito Uezono, Masanori Sasaki, Komyo Eto .  Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmoset .  Journal of toxicological sciences37 ( 4 ) 749 - 763   2012.8Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmosetReviewed

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  • Hideyuki Yamatoya, Hiroaki Kawaguchi, Kanako Yajima, Hidetomi Kadokura, Tsuyoshi Yoshikawa, Rie Yamashita, Mitsuya Shiraishi, Atsushi Miyamoto, Noriaki Miyoshi .  Data on Wistar Hannover rats from a general toxicity study .  Experimental animals61 ( 4 ) 467 - 476   2012.7Data on Wistar Hannover rats from a general toxicity studyReviewed

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  • Fukuko Matsumoto, Yutaka Watanabe, Takeshi Obi, M. Z. Islam, Emi Yamazaki-Himeno, Mitsuya Shiraishi, Atsushi Miyamoto. .  Characterization of 5-hydroxytryptamine-induced contraction and acetylcholine-induced relaxation in isolated chicken basilar artery .  Poultry science91 ( 5 ) 1158 - 1164   2012.5Characterization of 5-hydroxytryptamine-induced contraction and acetylcholine-induced relaxation in isolated chicken basilar arteryReviewed

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  • Hideyuki Yamatoya, Hiroaki Kawaguchi, Takeshi Fukuda, Hidetomi Kadokura, Rie Yamashita, Tsuyoshi Yoshikawa, Mitsuya Shiraishi, Atsushi Miyamoto, Noriaki Miyoshi .  Data on Wistar Hannover rats from an immunotoxicity study .  Experimental animals61 ( 2 ) 171 - 175   2012.4Data on Wistar Hannover rats from an immunotoxicity studyReviewed

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  • Atsuhiro Tanabe, Mitsuya Shiraishi, Manabu Negishi, Naoaki Saito, Mitsuo Tanabe, Yasuharu Sasaki .  MARCKS dephosphorylation is involved in bradykinin-induced neurite outgrowth in neuroblastoma SH-SY5Y cells .  Journal of cellular physiology227   618 - 629   2012.2MARCKS dephosphorylation is involved in bradykinin-induced neurite outgrowth in neuroblastoma SH-SY5Y cellsReviewed

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  • Miki Nakashima, Yukiko Shigekuni, Takeshi Obi, Mitsuya Shiraishi, Atsushi Miyamoto, Hideo Yamasaki, Takeomi Etoh, Sumio Iwai. .  Nitric oxide-dependent hypotensive effects of wax gourd juice .  Journal of ethnopharmacology138 ( 2 ) 404 - 407   2011.11Nitric oxide-dependent hypotensive effects of wax gourd juiceReviewed

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  • Mitsuya Shiraishi, Eisuke Tani, Atsushi Miyamoto .  Modulation of rabbit platelet aggregation and calcium mobilization by platelet cholesterol content .  Journal of veterinary medical science72 ( 3 ) 285 - 292   2010.3Modulation of rabbit platelet aggregation and calcium mobilization by platelet cholesterol contentReviewed

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  • Hiroki Yamaguchi, Mitsuya Shiraishi, Kiyoko Fukami, Atsuhiro Tanabe, Yuri Ikeda-Matsuo, Yasuhito Naito, Yasuharu Sasaki .  MARCKS regulates lamellipodia formation induced by IGF-I via association with PIP2 and beta-actin at membrane microdomains .  Journal of cellular physiology220 ( 3 ) 748 - 755   2009.9MARCKS regulates lamellipodia formation induced by IGF-I via association with PIP2 and beta-actin at membrane microdomainsReviewed

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  • Daisaku Ueno, Akira Yabuki, Takeshi Obi, Mitsuya Shiraishi, Akira Nishio, Atsushi Miyamoto .  Characterization of bradykinin-induced endothelium-independent contraction in equine basilar artery .  Journal of cellular physiology32 ( 3 ) 264 - 270   2009.6Characterization of bradykinin-induced endothelium-independent contraction in equine basilar arteryReviewed

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  • Kosuke Takeya, Kathy Loutzenhiser, Mitsuya Shiraishi, Rodger Loutzenhiser, Michael P. Walsh .  A highly sensitive technique to measure myosin regulatory light chain phosphorylation: the first quantification in renal arterioles .  American journal of physiology. Renal physiology294 ( 6 ) F1487 - F1492   2008.6A highly sensitive technique to measure myosin regulatory light chain phosphorylation: the first quantification in renal arteriolesReviewed

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  • Tadatsune Okuno, Akira Yabuki, Mitsuya Shiraishi, Takeshi Obi, Atsushi Miyamoto .  Histamine-induced modulation of vascular tone in the isolated chicken basilar artery: A possible involvement of endothelium .  Comparative biochemistry and physiology - part C: toxicology & pharmacology 147 ( 3 ) 339 - 344   2008.4Histamine-induced modulation of vascular tone in the isolated chicken basilar artery: A possible involvement of endotheliumReviewed

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  • Narihiro Yoshinaga,Tadatune Okuno, Yutaka Watanabe, Tsukasa Matsumoto, Mitsuya Shiraishi, Takeshi Obi, Akira Yabuki, Atsushi Miyamoto .  Vasomotor effects of noradrenaline, acetylcholine, histamine, 5-hydroxytryptamine and bradykinin on snake (Trimeresurus flavoviridis) basilar arteries .  Comparative biochemistry and physiology - part C: toxicology & pharmacology 146 ( 4 ) 478 - 483   2007.11Vasomotor effects of noradrenaline, acetylcholine, histamine, 5-hydroxytryptamine and bradykinin on snake (Trimeresurus flavoviridis) basilar arteriesReviewed

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  • Mitsuya Shiraishi, Atsuhiro Tanabe, Naoaki Saito, Yasuharu Sasaki .  Unphosphorylated MARCKS is involved in neurite initiation induced by insulin-like growth factor-I in SH-SY5Y cells .  Journal of cellular physiology209 ( 3 ) 1029 - 1038   2006.12Unphosphorylated MARCKS is involved in neurite initiation induced by insulin-like growth factor-I in SH-SY5Y cellsReviewed

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  • Ruriko Koto, Masatoshi Imamura, Chie Watanabe, Satoshi Obayashi, Mitsuya Shiraishi, Yasuharu Sasaki, Hiroshi Azuma .  Linalyl acetate as a major ingredient of lavender essential oil relaxes the rabbit vascular smooth muscle through dephosphorylation of myosin light chain .  Journal of cardiovascular pharmacology48 ( 1 ) 850 - 856   2006.7Linalyl acetate as a major ingredient of lavender essential oil relaxes the rabbit vascular smooth muscle through dephosphorylation of myosin light chainReviewed

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  • Greg A. Knock, Anushika S. De Silva, Vladimir A. Snetkov, Richard Siow, Gavin D. Thomas, Mitsuya Shiraishi, Michael P. Walsh, Jeremy P. T. Ward, Philip I. Aaronson .  Modulation of PGF(2 alpha)- and hypoxia-induced contraction of rat intrapulmonary artery by p38 MAPK inhibition: a nitric oxide-dependent mechanism .  American journal of physiology. Lung cellular and molecular physiology 289 ( 6 ) L1039 - L1048   2005.12Modulation of PGF(2 alpha)- and hypoxia-induced contraction of rat intrapulmonary artery by p38 MAPK inhibition: a nitric oxide-dependent mechanismReviewed

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  • Mitsuya Shiraishi, Rodger D. Loutzenhiser, Michael P. Walsh .  A highly sensitive method for quantification of myosin light chain phosphorylation by capillary isoelectric focusing with laser-induced fluorescence detection .  Electrophoresis26 ( 3 ) 571 - 580   2005.2A highly sensitive method for quantification of myosin light chain phosphorylation by capillary isoelectric focusing with laser-induced fluorescence detectionReviewed

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  • Erika Shimomura, Mitsuya Shiraishi, Takahiro Iwanaga, Minoru Seto, Yasuharu Sasaki, Masahiro Ikeda, Katsuaki Ito .  Inhibition of protein kinase C-mediated contraction by Rho kinase inhibitor fasudil in rabbit aorta .  Naunyn-Schmiedeberg's archives of pharmacology370 ( 5 ) 414 - 422   2004.11Inhibition of protein kinase C-mediated contraction by Rho kinase inhibitor fasudil in rabbit aortaReviewed

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  • Mitsuya Shiraishi, Xuemei Wang, Michael P. Walsh, Gary Kargacin, Kathy Loutzenhiser, Rodger Loutzenhiser .  Myosin heavy chain expression in renal afferent and efferent arterioles: relationship to contractile kinetics and function .  FASEB journal17 ( 13 ) 2284 - 2286   2003.10Myosin heavy chain expression in renal afferent and efferent arterioles: relationship to contractile kinetics and functionReviewed

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  • Katsuaki Ito, Erika Shimomura, Takahiro Iwanaga, Mitsuya Shiraishi, Kazutoshi Shindo, Junji Nakamura, Hiromitsu Nagumo, Minoru Seto, Yasuharu Sasaki, Yoh Takuwa .  Essential role of rho kinase in the Ca2+ sensitization of prostaglandin F-2 alpha-induced contraction of rabbit aortae .  Journal of physiology (London)546 ( 3 ) 823 - 836   2003.2Essential role of rho kinase in the Ca2+ sensitization of prostaglandin F-2 alpha-induced contraction of rabbit aortaeReviewed

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  • Mitsuya Shiraishi, Sachiko Kawashima, Masaaki Moroi, Yangchol Shin, Takashi Morita, Yoichiro Horii, Masahiro Ikeda, Katsuaki Ito .  A defect in collagen receptor-Ca2+ signaling system in platelets from cattle with Chediak-Higashi syndrome .  Thrombosis and haemostasis87 ( 2 ) 334 - 341   2002.2A defect in collagen receptor-Ca2+ signaling system in platelets from cattle with Chediak-Higashi syndromeReviewed

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  • Mitsuya Shiraishi, Masahiro Ikeda, Takeshi Fujishiro, Kiichi Fukuyama, Katsuaki Ito .  Characteristics of collagen-induced Ca2+ mobilization in bovine platelets .  Cell calcium27 ( 1 ) 53 - 60   2000.1Characteristics of collagen-induced Ca2+ mobilization in bovine plateletsReviewed

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  • Mitsuya Shiraishi, Masahiro Ikeda, Hiroyuki Ogawa, Chien-HsienTu, Katsuaki Ito .  Impaired cytosolic calcium mobilization and aggregation in response to collagen in platelets from Japanese Black cattle with Chediak-Higashi syndrome .  American journal of veterinary research59 ( 6 ) 744 - 749   1998.6Impaired cytosolic calcium mobilization and aggregation in response to collagen in platelets from Japanese Black cattle with Chediak-Higashi syndromeReviewed

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MISC

  • 【マグネシウム代謝-その新たな臨床的意義】基礎と総論　マグネシウム濃度と生命予後との関連性　血圧と飲料水を中心に

    鈴木 舞, 新居 朋昭, 松岡 由紀, 澤 佐和子, 白石 光也, 宮本 篤

    腎と透析   86 ( 2 )   163 - 166   2019.2

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    Language：Japanese   Publisher：(株)東京医学社  

  • Platelet dysfunction in Chediak-Higashi syndrome-affected cattle Reviewed

    Mitsuya Shiraishi, Hiroyuki Ogawa, Masahiro Ikeda, Sachiko Kawashima, Katsuaki Ito

    Journal of veterinary medical science   64 ( 9 )   751 - 760   2002.4

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Presentations

  • 呉思遠、大田和朋紀、宇野泰弘、白石光也、宮本篤   ニワトリ脳底動脈のβ受容体を介した弛緩反応に関与する受容体サブタイプとその特性  

    第165回日本獣医学会学術集会  2022.9 

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    Event date： 2022.9

    Language：Japanese   Presentation type：Oral presentation (general)  

  • 辻尾祐志、白石光也、松元光春   コロナ禍における鹿児島大学の獣医解剖・組織学教育の実施方法と今後の取り組み   Invited

    第165回日本獣医学会学術集会  2022.9 

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    Event date： 2022.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  • 大田和朋紀、呉思遠、白石光也、宇野泰広、宮本篤   近接する2島である奄美大島と徳之島に生息する同種のハブの胸大動脈でみられた血管反応性の違い  

    第165回日本獣医学会学術集会  2022.9 

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    Event date： 2022.9

    Language：Japanese   Presentation type：Oral presentation (general)  

  • Kiyotada Naitou, Honoka Iwashita, Hiromi H. Ueda, Mitsuya Shiraishi, Yoshikazu Fujimoto, Kazuhiro Horii, Tomoya Sawamura, Takahiko Shiina and Yasutake Shimizu   Spinal Substance P Related To Defecation Reflexes And Enhanced Colorectal Motility.   International conference

    International Society for Autonomic Neuroscience 2022  2022.9  nternational Society of Autonomic Neuroscience

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    Event date： 2022.9

    Language：English   Presentation type：Poster presentation  

    Venue：Cairns Convention Centre   Country：Australia  

  • 植田大海、内藤清惟、白石光也、児島一州、正谷達謄、 椎名貴彦、志水泰武   脊髄排便中枢においてα-MSH は 1 型メラノコルチン受容体 （MC1R）を介して大腸運動を促進する  

    第31回日本病態生理学会大会  2022.8 

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    Event date： 2022.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岐阜大学 柳戸キャンパス  

    Other Link： http://31st-pathophysiology.kenkyuukai.jp/images/sys/information/20220720133221-0D6DDB2185BE343443736EA0F05E67C1AB13F8CEDA321C0D982C01327B7FD039.pdf

  • Siyuan Wu, Ryoya Sekio, Henry Smith, Mitsuya Shiraishi, Atsushi Miyamoto   Noradrenaline-induced basilar artery vasomotor response also varies in avian species (chickens and ducks)  

    第95回日本薬理学会年会  2022.3 

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    Event date： 2022.3

    Language：English   Presentation type：Oral presentation (general)  

    Other Link： https://pharmacology.main.jp/jps95/download/pdf/oral_0309.pdf

  • 宮本篤、呉思遠、大田和朋紀、関尾椋哉、宇野泰弘、白石光也、Md. Zahorul Islam   摘出脳底動脈におけるヒスタミン血管反応性の動物種差  

    第23回日本ヒスタミン学会  2022.1 

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    Event date： 2022.1

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都大学医学部　芝蘭会館別館  

  • 鈴木 舞, 白石 光也, 松岡 由紀, 澤 佐和子, 森 真理, 森 英樹, 家森 幸男, 宮本 篤   徳之島伊仙町の飲料水を用いて調製したクレブスリンゲル液の脳血管反応性に及ぼす影響  

    第37回日本マグネシウム学会  2017.11  (一社)日本マグネシウム学会

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    Event date： 2017.11

    Language：Japanese   Presentation type：Oral presentation (general)  

  • 内藤 清惟, 岩下 朋乃樺, 植田 大海, 白石 光也, 藤本 佳万, 椎名 貴彦, 志水 泰武   脊髄排便中枢におけるサブスタンスPの大腸運動促進作用  

    日本獣医学会学術集会講演要旨集  2021.9  (公社)日本獣医学会

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    Language：Japanese  

  • 植田 大海, 内藤 清惟, 白石 光也, 児島 一州, 正谷 達謄, 椎名 貴彦, 志水 泰武   脊髄排便中枢においてα-MSHは1型メラノコルチン受容体(MC1R)を介して大腸運動を促進する  

    日本病態生理学会雑誌  2022.7  日本病態生理学会

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    Language：Japanese  

  • 牧野 遥, 正谷 達謄, 藤本 佳万, 内藤 清惟, 白石 光也   未分化型および分化型ヒト由来培養神経細胞におけるウイルス感受性の比較  

    日本獣医学会学術集会講演要旨集  2021.9  (公社)日本獣医学会

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  • 呉 思遠, 大田和 朋紀, 宇野 泰広, 白石 光也, 宮本 篤   ニワトリ脳底動脈のβ受容体を介した弛緩反応に関与する受容体サブタイプとその特性  

    日本獣医学会学術集会講演要旨集  2022.9  (公社)日本獣医学会

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    Language：Japanese  

  • 辻尾 祐志, 白石 光也, 松元 光春   コロナ禍及びこれからの獣医解剖学実習　コロナ禍における鹿児島大学の獣医解剖・組織学教育の実施方法と今後の取り組み  

    日本獣医学会学術集会講演要旨集  2022.9  (公社)日本獣医学会

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    Language：Japanese  

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Research Projects

  • Role of MARCKS protein on developmental neurotoxicity of toxic metals

    Grant number：19K06404  2019.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\3770000 （ Direct Cost: \2900000 、 Indirect Cost：\870000 ）

  • Roles of MARCKS in platelets, smooth muscle and endothelial cells in haemostasis and thrombosis

    Grant number：26450407  2014.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    SHIRAISHI Mitsuya, MIYAMOTO Atsushi

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    Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）

    We studied the role of MARCKS protein in endothelial cells, smooth muscle cells, and platelets. In endothelial cells, MARCKS protein was involved in cell proliferation, cell migration, and nitric oxide production. Furthermore, participation of MARCKS in abnormal cell functions of diabetes or methylmercury intoxication model animals was suggested. In addition, MARCKS was expressed in smooth muscle cells and platelets, and the role of MARCKS in these cells was indicated.