Language：Japanese   Publisher：Research in Veterinary Science  

Dogs with osteoarthritis (OA) may benefit from stromal cell therapies, but there is a lack of such established therapies. Accordingly, we investigated the feasibility and safety of synovial fluid (SF)-derived mesenchymal stromal cell (MSC) transplantation for canine OA. MSCs were isolated from SF (n = 15) or by joint lavage (JLF; n = 12) from the knee joints of dogs undergoing orthopedic surgery (n = 25), and then cultured on a dish (passage 1) and a polyethylene terephthalate (PET) nonwoven fabric scaffold (passage 2). We obtained 2166 ± 1177 (SF) or 1640 ± 1064 (JLF) × 10⁴ primary cells and 18,673 ± 8500 (SF) or 16,870 ± 3348 (JLF) × 10⁴ passaged cells, surpassing the target number. Passaged cells were morphologically uniform, self-proliferating, CD29, CD44, CD90, and CD105-positive, and CD31-negative cells, with the ability to differentiate into osteoblasts, chondrocytes, and adipocytes. Healthy dogs (n = 3) then received a 1-mL allogenic MSC suspension (at 1×, 5×, and 10 × 10⁷ cells), harvested and prepared with the same method, by intraarticular injection (with contralateral knees as negative controls), and were evaluated for adverse clinical signs, hematologic and biochemical parameters periodically, and joint images and pathology at day 90. No abnormal findings were found in any dogs, confirming the safety of allogeneic MSC transplantation

DOI： 10.1016/j.rvsc.2025.105897

Scopus

PubMed

Language：English   Publisher：Japanese Society of Veterinary Anesthesia and Surgery  

<p>Malignant tumors arising from thoracic wall may require extensive resection, including ribs. The major complication following rib resection is the development of a flail chest, and resection of more than six ribs is not recommended. We performed a resection with six ribs for a liposarcoma in the thoracic wall of a dog, followed by reconstruction of two cephalic ribs with a titanium plate. There was no postoperative flail chest and the patient’s respiratory status was stable. Rib reconstruction with plates was considered to allow functional reconstruction of the thoracic wall after extensive mass resection.</p>

DOI： 10.2327/jjvas.54.53

CiNii Research

Language：Japanese   Publisher：(一社)日本獣医麻酔外科学会  

体壁の悪性腫瘍は肋骨を含めた広範囲な切除が必要な場合がある。肋骨切除による主な合併症はフレイルチェストの発生であり、6本以上の肋骨切除は推奨されていない。犬の胸壁に発生した脂肪肉腫に対して6本の肋骨を含めた切除を行った後、チタンプレートで頭側2本の肋骨再建を実施した。術後フレイルチェストの発生はなく、呼吸状態も安定していた。プレートによる肋骨再建により機能的な胸壁再建を可能にすると考えられた。(著者抄録)

Language：Japanese   Publisher：(公社)日本獣医師会  

本研究は,細菌性乳房炎を発症したホルスタイン種乳牛の乳汁から分離された大腸菌(E.coli)23株のバイオフィルム(BF)形成能を調査し,臨床的に用いられる抗菌薬5種類のBFに対する作用を明らかにすることを目的とした.供試細菌株23株中21株がBFを形成した.最小殺菌濃度及び最小BF形成阻害濃度は,フルオロキノロン系抗菌薬において低値を示した.最小BF撲滅濃度はすべての供試抗菌薬において高値を示した.よって,フルオロキノロン系抗菌薬は全身投与により乳房内に感染したE.coliの増殖を抑制可能であるとともに,BF形成も抑制可能であると推察された.また,いずれの抗菌薬においてもBF形成後の効果は期待できないと考えられた.(著者抄録)

Language：Japanese   Publisher：Japan Veterinary Medical Association  

<p>This study evaluated the biofilm (BF)-forming ability of <i>Escherichia coli</i> isolated from the milk of dairy cows with bacterial mastitis and clarified the effects of five clinically used antibacterial agents on biofilms. Twenty-one of the 23 test strains formed biofilms. The minimum bactericidal concentration (MBC) and minimum BF formation inhibitory concentration (MBIC) were low for fluoroquinolones. The minimum BF eradication concentration (MBEC) was high for all of the tested antimicrobial agents. From the results, systemic administration of fluoroquinolone antibiotics can suppress the growth of <i>E. coli</i> in the udder and also suppress BF formation. The inhibition of BF formation was considered impossible for CEZ and TYL. In addition, it was considered difficult to treat infections with any antibacterial drug after BF formation. From the above, fluoroquinolone antibacterial agents are considered to be effective in the treatment of mastitis caused by <i>E. coli</i>, and the importance of initial treatment with sensitive antibacterial agents before BF formation was reiterated.</p>

DOI： 10.12935/jvma.75.e191

CiNii Research

Language：Japanese  

Language：Japanese