担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Fishes  

This study investigated the influence of dietary supplementation with Bacillus subtilis natto (BSN) on various physiological parameters in red sea bream (Pagrus major). Key areas of focus included growth performance, enzymatic activity related to digestion, blood biochemical markers, immune function, and intestinal microorganisms. Fish were fed diets containing the following five levels of BSN: 0 (BN0), 1 × 107 (BN1), 1 × 108 (BN2), 1 × 109 (BN3), and 1 × 1010 (BN4) CFU kg−1 for 56 days. Fish in the BN3 and BN4 groups exhibited significantly enhanced growth performance compared to the BN0 group (p < 0.05). Additionally, the activities of amylase and protease were markedly higher in the BN3 and BN4 groups (p < 0.05), while lipase activity was significantly elevated in fish fed the BN3 diet (p < 0.05). Plasma total protein levels also showed a significant increase in the BN3 group (p < 0.05). Hematocrit values were significantly improved in the BN2, BN3, and BN4 groups (p < 0.05). The intestinal microbiota of the BN4 group revealed a significant increase in the populations of B. subtilis and Lactobacillus, along with a notable decrease in Escherichia coli, compared to all other groups (p < 0.05). The observed improvements in specific growth rate and the upregulation of growth-related gene expression further highlight the potential of BSN to enhance the growth performance of red sea bream. In conclusion, dietary supplementation with BSN at 1 × 109 and 1 × 1010 CFU kg−1 shows promise in improving the growth, health, and immune response of red sea bream.

DOI： 10.3390/fishes9110446

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担当区分：筆頭著者,　最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： doi.org/10.3390/fermentation7040306

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Marine Pollution Bulletin  

Siver nanoparticles (AgNPs) are widely used worldwide due to their diverse application history; however, concerns are raising about their potential toxicity in aquatic organisms such as fish. Therefore, the current study aims to evaluate the impact of greenly synthesized AgNPs on the health of Himalayan Mahseer ( Tor putitora ). Selected fish were exposed to different groups based on the concentrations of AgNPs (0.5 mg/L, 1 mg/L, and 2 mg/L) for two weeks, and behavioral, haematological, biochemical, oxidative stress, histopathological and genotoxic effects were assessed. Significant behavioral alterations, including abnormal operculum movement, irregular swimming patterns, convulsions, and fin tilting were observed at the highest concentration (2 mg/L). Haematological analysis showed a notable decline ( p < 0.05) in erythrocytes, hemoglobin, hematocrit, leukocytes, and mean corpuscular hemoglobin concentration, with an increase in mean corpuscular volume. Biochemical findings indicated elevated levels of enzymes, while calcium, potassium, phosphorus, and iron decreased in all treated groups. Metabolites increased, whereas triglycerides decreased. The results on oxidative stress biomarkers and antioxidant enzymes in gils, liver and kidney tissues resulted in significantly ( p < 0.05) higher quantity of oxidative stress biomarkers, while lower concentrations of antioxidant enzymes in treated fish. Histopathological analysis demonstrated the damaged gill structure with cartilage core destruction, necrosis and telangiectasia, while the liver tissues included blood coagulation, pyknotic nuclei, and melanomacrophages formation. In the intestinal tissues, abnormalities noted include serosa destruction, detached villi, villi fusion, and necrosis. Genotoxicity analysis using the comet assay revealed significant DNA damage in erythrocytes, especially at 2 mg/L. These findings demonstrate that greenly synthesized AgNPs can cause severe physiological and genetic alterations in fish, highlighting their potential risk to aquatic ecosystems.

DOI： 10.1016/j.marpolbul.2026.119858

DOI： 10.1016/j.marpolbul.2026.119858

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その他リンク： https://doi.org/10.1016/j.marpolbul.2026.119858

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Annals of Animal Science  

Most probiotic yeast supplement in fish exhibit beneficial effect at ≤1% of the dietary proportion. This study aimed at evaluating the specific effects of Lypomyces starkeyi CB1807 yeast strain supplemented at ≤1% of dietary proportion on the performance of juvenile red sea bream (Pagrus major, 1.9 ± 0.04 g). Five diets were supplemented with yeast at graded levels of 0% (control diet 'CD1'), 0.05% (D2), 0.1% (D3), 0.5% (D4), and 1.0% (D5). After 45 days of feeding trial, significant (P<0.05) improvement was detected on final body weight (FBW) and body weight gain (BWG) in fish fed D3 and D5 compared to control. Low values of total cholesterol (T-Cho) and aspartate aminotransferase (AST) were recorded in fish groups fed on D2, D4, and D5, respectively. Fish fed on D3, D4 and D5 diets showed high (P<0.05) values of serum, mucus and liver lysozyme compared to control. Fish fed on D5 showed high values of total immunoglobulin (Ig) compared to control. Fish fed on D2 showed strong correlation with biological antioxidant activity (BAP), superoxide dismutase (SOD) and catalase activity (CAT). The biological antioxidant potential (BAP) activity in fish fed on D2 was significantly higher compared to control (P<0.05). The reactive oxygen metabolites (d-ROM) were significantly lower in fish fed on D2 and D3 compared to CD1 (P<0.05). Peroxidase activity was improved significantly (P<0.05) in fish fed on D3, D4 and D5 compared to control. The tolerance ability (LT50) of fish fed on D5 against low salinity stress was significantly higher compared to control (P<0.05). It was concluded that dietary benefits of spent L. starkeyi yeast at ≤1% showed considerable improvement in antioxidant capacity in red sea bream, P. major.

DOI： 10.2478/aoas-2022-0012

DOI： 10.2478/aoas-2022-0012

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その他リンク： https://doi.org/10.2478/aoas-2022-0012

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Aquaculture Reports  

The current work assessed the inclusion impacts of oilseed camelina meal (CM) as a protein source in red sea bream (Pagrus major) diets. A 45 day assessment period with 180 juveniles (6.47 ± 0.17 g) were allocated in triplicates to 4 experimental groups and fed formulated diets in which fish meal (FM) was subrogated at graded series of 0% (T1), 20.5% soybean meal (T2), 20.5% camelina meal (T3), and 33% camelina meal (T4). No noticeable alterations were observed in specific growth rate, feed intake, survival rate, hepatosomatic index, and Fulton's condition factor among the experimental groups. Fish fed the T4 diet showed considerably reduced (P < 0.05) final weight and body protein content when compared to those fed T2, T3, and basal diets. Plasma biochemical parameters show no differences (P > 0.05) in glucose, total bilirubin, total protein, total cholesterol, triglyceride, glutamyl oxaloacetic transaminase, and glutamic pyruvate transaminase. Hematocrit levels decreased noticeably (P < 0.05) in fish groups fed camelina meal in comparison to the control group. Liver and intestinal histology showed a healthy status and an increase in villus length and goblet cell number in camelina groups. Fish fed the T4 diet displayed higher expression levels (P < 0.05) of relative mRNA interleukin 1 beta (IL-1b) and tumor necrosis factor-alpha (TNF-α) in comparison to other groups. The inclusion of camelina meal (up to 20%) in red sea bream diets produced similar outcomes to specimens fed fish meal and soybean meal.

DOI： 10.1016/j.aqrep.2022.101175

DOI： 10.1016/j.aqrep.2022.101175

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その他リンク： https://doi.org/10.1016/j.aqrep.2022.101175

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Aquaculture Nutrition  

A 50-day feeding trial was conducted to evaluate the efficiency of single and mixed strains of probiotic bacteria supplements on juvenile red sea bream (Pagrus major). The study investigated the growth, digestibility, hemato-biochemistry, antioxidant, immune, immune, and growth gene expression and stress responses of P. major. Three hundred juvenile P. major (21:56 ± 0:09 g) were randomly distributed into fifteen 200-L polyethylene tanks (20 fish per tank) in triplicate of 5 treatments designated as D1 = the basal diet; Streptococcus faecalis (SF) T − 110 5 × 106 cfu/g diet and Bacillus amyloliquefaciens (BA) TOA 5001 5 × 105 cfu/g diet in the second group (D2) at 0.2%; mix SF T − 110 1 × 106 cfu/g diet, Lactobacillus plantarum (LP) TO − A 4 × 105 cfu/g diet, Bacillus mesentericus (BM) TO − A 1 × 102 cfu/g diet, BA TOA5001 5 × 105 cfu/g diet in the third group (D3) at 1%; mix SF T − 110 1 × 106 cfu/g diet, LP TO − A 4 × 105 cfu/g diet, and BM TO − A 1 × 102 cfu/g diet in fourth group (D4) at 0.5%; and single strain BA TOA5001 5×105 cfu/g diet in the fifth group (D5) at 0.5% of dietary proportion. Results showed that D2 and D3 fish groups exhibit better performance, followed by D4, D5, and control group D1. This finding demonstrated that the use of BA in mix strain probiotic bacteria diet (D2, D3) had improved immune response, antioxidant enzymes, immunity (TNF-a, IL-1b), and growth-related (IGF-1, IGF-2) mRNA expression of juvenile P. major compared to the mix strain D4, single strain D5, and the control D1. Furthermore, single strain D5 and mix strain D4 also exhibit relatively better immune responses in P. major than in control D1. Considering the overall fish performances, mix strain SF+BA (D2) and mix strain SF+BM+LP+BA (D3) were the recommended potential mix strain probiotic bacteria supplement for P. major and may be useful also for other related aquatic species.

DOI： 10.1155/2022/8968494

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その他リンク： https://doi.org/10.1155%2F2022%2F8968494

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Animals  

A 60-day experiment was designed to assess the effect of different ratios of fish meal (FM): camelina meal plant protein (CM) on growth response and relative gene expression of growth-pro-moting factors, feed utilization potency, digestive enzymes activities, apparent digestibility (ADC), stress response, non-specific immunity of Pagrus major. Four isonitrogenous (490.7 g/kg of crude protein) and isolipidic (91.5 g/kg total lipid) experimental diets were formulated and designated as camelina meal (CM0), soyabean meal (SBM20.5), CM20.5, and CM33 based on protein contents. At the end of the feed trial, significantly higher (p < 0.05) weight gain, specific growth rate, and feed intake but lower feed conversion ratio were recorded in fish fed CM0, SBM20.5, and CM20.5 than fish fed CM33. The lowest growth, feed utilization, enzyme activity, and digestibility were recorded in fish fed CM33. Significantly higher pepsin, amylase, and protease activities were observed in fish fed CM0, SBM20.5, and CM20.5 diets than fish fed CM33. The highest ADC of protein was recorded in fish fed CM0, SBM20.5, and CM20.5 diets. Hematocrit levels were depressed CM33 while total serum protein, total cholesterol, triglyceride, blood urea nitrogen, total bilirubin, aspartate ami-notransferase, and alanine aminotransferase were not significantly changed by the inclusion of CM. Non-specific immune variables (lysozyme activity, peroxidase activity in serum and nitro blue te-trazolium) in fish fed CM0, SBM20.5, and CM20.5 were significantly higher than in fish fed CM33 diet. The superoxide dismutase of fish fed CM20.5 was not significantly different from CM0 and SBM20.5 (p > 0.05). Catalase and low salinity stress test show that CM0, SBM20.5, and CM20.5 were not significantly (p > 0.05) different, while CM33 was significantly lower than the rest of the diets. TBARs show that CM20.5 and CM33 diets were significantly different (p < 0.05), but CM20.5 was not significantly different from SBM20.5. Significantly higher hepatic IGF-1 and IGF-2 mRNA expression was found in fish-fed diet groups CM0, SBM20.5, and CM20.5 than fish fed CM33. The present study indicated that the addition of CM up 205 kg/kg to diet maintains growth, digestive enzymes, nutrient digestibility, immunity, stress resistance, and feed utilization efficiency of red sea bream.

DOI： 10.3390/ani11113118

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その他リンク： https://doi.org/10.3390/ani11113118

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Animals  

A 56-day feeding trial to evaluate the responses of red seabream (initial weight: 1.8 ± 0.02 g) to the substitution of fish oil (FO) with camelina oil (CO) at different ratios was conducted. The control diet formulated at 46% CP (6F0C) contained only FO without CO; from the second to the fifth diet, the FO was substituted with CO at rates of 5:1 (5F1C), 4:2 (4F2C), 3:3 (3F3C), 2:4 (2F4C), and 0:6 (0F6C). The results of the present study showed that up to full substitution of FO with CO showed no significant effect on growth variables BW = 26.2 g–28.3 g), body weight gain (BWG = 1275.5– 1365.3%), specific growth rate (SGR = 4.6–4.7), feed intake (FI = 25.6–27.8), feed conversion ratio (FCR = 1.0–1.1), biometric indices condition factor (CF = 2.2–2.4), hepatosomatic index (HSI = 0.9–1.1), viscerasomatic index (VSI = 7.5–9.5), and survival rates (SR = 82.2–100) with different FO substitution levels with CO. Similarly, there were no significant differences (p < 0.05) found in the whole-body composition except for the crude lipid content, and the highest value was observed in the control group (291 g/kg) compared to the other groups FO5CO1 (232 k/kg), FO4CO2 (212 g/kg), FO2CO4 (232 g/kg) and FO0CO6 (244 g/kg). Blood chemistry levels were not influenced in response to test diets: hematocrit (36–33%), glucose (Glu = 78.3–71.3 mg/dL), total protein (T-pro = 3.1–3.8 g/dL), total cholesterol (T-Chol = 196.0–241 mg/dL), blood urea nitrogen (BUN = 9.0–14.6 mg/dL), total bilirubin (T-Bil = 0.4–0.5 mg/dL), triglyceride (TG = 393.3–497.6 mg/dL), alanine aminotransferase test (ALT = 50–65.5 UL/L), aspartate aminotransferase test (AST = 38–69.3 UL/L). A remarkable modulation was observed in catalase (CAT) and superoxide dismutase (SOD) activities in the liver, as CAT and SOD values were lower with the complete FO substitution with CO (0F6C), and the highest values were observed in the control and (4F2C). This study indicates that red seabream may have the ability to maintain LC-PUFAs between tissues and diets, and CO substitution of FO could improve both lipid metabolism and oxidation resistance as well as maintain digestibility. In conclusion, dietary FO can be replaced up to 100% or 95% by CO in the diets of red seabream as long as n-3 HUFA, EPA, and DHA are incorporated at the recommended level.

DOI： 10.3390/ani11071990

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その他リンク： https://doi.org/10.3390/ani11071990

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1155/2021/9957723

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記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Annals of Animal Science  

The beneficial effects of Bacillus subtilis C-3102 and Saccharomyces cerevisiae type 1 were tested in red sea bream (Pagrus major) feeds. A basal diet (control) and two other diets were prepared by supplementation with B. subtilis C-3102 (PB) or S. cerevisiae type 1 (PY). After 60 days, both probiotic-supplemented groups exhibited significant enhancement in growth performance, the protein efficiency ratio (PER), and digestive enzyme secretion (protease and amylase) compared to the control group (P<0.05). The anterior, middle, and posterior parts of the intestines exhibited significantly increased values of intestinal fold height (hF), enterocyte height (hE), and microvillus height (hMV) in fish fed PB-or PY-supplemented diets (P<0.05). Serum peroxidase, anti-protease, and bactericidal activities were enhanced significantly in both probiotic-treated groups compared to the control group (P<0.05). Serum and mucus lysozyme activities improved significantly in the PB group compared to the control group (P<0.05). Catalase activity was also significantly decreased in both probiotic groups, with relatively lower activity observed in the PY group (P<0.05). Both probiotic groups showed considerably increased tolerance to freshwater exposure (P<0.05). In conclusion, B. subtilis C-3102 and S. cerevisiae type 1 can be used as functional probiotics to enhance the growth performance, digestion capacity, gut morphology, immune response, and stress resistance of the red sea bream with relatively higher efficiency by B. subtilis C-3102.

DOI： 10.2478/aoas-2020-0075

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その他リンク： https://doi.org/10.2478/aoas-2020-0075

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/asj.13291

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本暖地畜産学会  

<p>本研究ではブロイラー用飼料としての加熱乾燥サツマイモ加工残さ（D-SPW）の栄養評価を行った．生のサツマイモ加工残さを加熱真空乾燥（72℃，6 時間，絶対圧 15 kpa）して D-SPW を調製した．14 日齢の雄ブロイラー 16 羽を対照区および D-SPW 区に分け，トウモロコシおよび大豆粕を主原料とする前期飼料（14 〜 28 日齢；CP，20％；ME，13.0 MJ/kg）および後期飼料（29 〜42 日齢；CP，18％；ME，13.0 MJ/kg）を自由摂取させた．D-SPW 区のブロイラーには，前期および後期飼料にそれぞれ 28.4％および 25.5％の D-SPW を配合して与えた．D-SPW 区のブロイラーの栄養素の代謝能力は，脂質を除いて，対照区より低かったが，全期間の成育成績に区間差はなかった．浅胸筋および大腿筋の pH は死後 1 および 48 時間で，D-SPW 区が対照区よりも高い値を示した．浅胸筋，大腿筋および腹腔内脂肪の黄色度は D-SPW 区が対照区より低い値を示し，黄色度が最も D-SPW の影響を受けやすいことが示された．明度および赤色度に対する D-SPW の影響は限定的であった．浅胸筋および大腿筋の脂質含量は，D-SPW 区が対照区より高い値を示した．以上より D-SPW は，トウモロコシの部分的な代替に適したブロイラー用の飼料資材であるが，脂肪含量が高くて黄色度の低い鶏肉が生産されることが示された．</p>

DOI： 10.11461/jwaras.62.107

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担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.fsi.2018.05.035

担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/are.13679

担当区分：筆頭著者,　最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.4995/wrs.2018.5917

その他リンク： https://doi.org/10.1016/j.anifeedsci.2014.01.014

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10695-016-0277-4

担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.anifeedsci.2014.01.014