Updated on 2022/06/22

写真a

 
Yoshimatsu Masayoshi
 
Organization
Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Sensory Organology Assistant Professor
Title
Assistant Professor

Degree

  • 医学博士 ( 2021.7   京都大学 )

Research Areas

  • Life Science / Otorhinolaryngology  / Regeneration of Larynx and Trachea

Education

  • Kyoto University

    2017.4 - 2021.3

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    Country: Japan

  • Kyushu University

    2005.4 - 2011.3

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    Country: Japan

 

Papers

  • Shinji Kaba, Yoshitaka Kawai, Yuki Tanigami, Hiroe Ohnishi, Tomoko Kita, Masayoshi Yoshimatsu, Koichi Omori, Yo Kishimoto .  PPARγ Agonist Attenuates Vocal Fold Fibrosis in Rats via Regulation of Macrophage Activation. .  The American Journal of Pathology   2022.2PPARγ Agonist Attenuates Vocal Fold Fibrosis in Rats via Regulation of Macrophage Activation.Reviewed International journal

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    Language:English   Publishing type:Research paper (scientific journal)  

    Macrophages aid in wound healing by changing their phenotype and can be a key driver of fibrosis. However, the contribution of macrophage phenotype to fibrosis following vocal fold injury remains unclear. Peroxisome proliferator-activated receptor-γ (PPARγ) is expressed mainly by macrophages during early wound healing and regulates the macrophage phenotype. This study aimed to evaluate the effects of pioglitazone, a PPARγ agonist, on the macrophage phenotype and fibrosis following vocal fold injury in rats. Pioglitazone was injected into the rats' vocal folds on days 1, 3, 5, and 7 after injury, and the vocal fold lamina propria was evaluated on days 4 and 56 after injury. Moreover, THP-1-derived macrophages were treated with pioglitazone, and the expression of pro-inflammatory cytokines under lipopolysaccharide/interferon-γ stimulation was analyzed. The results revealed that pioglitazone reduced the expression of Ccl2 both in vivo and in vitro. Furthermore, pioglitazone decreased the density of inducible nitric oxide synthase+ CD68+ macrophages and inhibited the expression of fibrosis-related factors on day 4 after injury. On day 56 after injury, pioglitazone inhibited fibrosis, tissue contracture, and hyaluronic acid loss in a PPARγ-dependent manner. These results indicate that PPARγ activation could inhibit accumulation of inflammatory macrophages and improve tissue repair. Considered together, these findings imply that inflammatory macrophages play a key role in vocal fold fibrosis.

    DOI: 10.1016/j.ajpath.2022.02.002

    DOI: 10.1016/j.ajpath.2022.02.002

    PubMed

  • Fumihiko Kuwata, Hiroe Ohnishi, Norio Yamamoto, Toshiaki Takezawa, Masaru Yamashita, Hideaki Okuyama, Yasuyuki Hayashi, Masayoshi Yoshimatsu, Yuji Kitada, Takeshi Tada, Masayoshi Kobayashi, Koichi Omori .  Transplantation of human iPS cell-derived airway cells on vitrigel membrane into rat nasal cavity. .  Tissue engineering. Part A   2021.11Transplantation of human iPS cell-derived airway cells on vitrigel membrane into rat nasal cavity.International journal

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    Language:English   Publishing type:Research paper (scientific journal)  

    The nasal mucosa functions as a frontline biological defense against various foreign substances and pathogens. Maintaining homeostasis of the nasal epithelium is necessary to promote good health. Nasal epithelia are constantly replaced under normal conditions. However, hereditary diseases, including primary ciliary dyskinesia and cystic fibrosis, can result in intractable dysfunction of the nasal mucosa. Since there is no treatment for this underlying condition, extrinsic manipulation is necessary to recover and maintain nasal epithelia in cases of hereditary diseases. In this study, we explored the use of airway epithelial cells (AECs), including multi-ciliated airway cells (MCACs), derived from human induced pluripotent stem cells (hiPSCs) on porcine atelocollagen vitrigel membranes, as a candidate of a therapeutic method for irreversible nasal epithelial disorders. To confirm the regenerative capacity of iPSC-derived AECs, we transplanted them into nasal cavities of nude rats. Although the transplanted cells were found within cysts isolated from the recipient nasal respiratory epithelia, they survived in some rats. Furthermore, the surviving cells were composed of multiple cell types similar to the human airway epithelia. The results could contribute to the development of novel transplantation-related technologies for the treatment of severe irreversible nasal epithelial disorders.

    DOI: 10.1089/ten.TEA.2021.0071

    PubMed

  • Fumihiko Kuwata, Hiroe Ohnishi, Norio Yamamoto, Toshiaki Takezawa, Masaru Yamashita, Hideaki Okuyama, Yasuyuki Hayashi, Masayoshi Yoshimatsu, Yuji Kitada, Takeshi Tada, Masayoshi Kobayashi, Koichi Omori .  Transplantation of human iPS cell-derived airway cells on vitrigel membrane into rat nasal cavity. .  Tissue Engineering. Part A   2021.11Transplantation of human iPS cell-derived airway cells on vitrigel membrane into rat nasal cavity. Reviewed

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/ten.TEA.2021.0071.

  • Masayoshi Yoshimatsu, Hiroe Ohnishi, Chengzhu Zhao, Yasuyuki Hayashi, Fumihiko Kuwata, Shinji Kaba, Hideaki Okuyama, Yoshitaka Kawai, Nao Hiwatashi, Yo Kishimoto, Tatsunori Sakamoto, Makoto Ikeya, Koichi Omori .  In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells. .  Stem Cell Research52   102233 - 102233   2021.2In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells. Reviewed International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    The laryngotracheal cartilage is a cardinal framework for the maintenance of the airway for breathing, which occasionally requires reconstruction. Because hyaline cartilage has a poor intrinsic regenerative ability, various regenerative approaches have been attempted to regenerate laryngotracheal cartilage. The use of autologous mesenchymal stem cells (MSCs) for cartilage regeneration has been widely investigated. However, long-term culture may limit proliferative capacity. Human-induced pluripotent stem cell-derived MSCs (iMSCs) can circumvent this problem due to their unlimited proliferative capacity. This study aimed to investigate the efficacy of iMSCs in the regeneration of thyroid cartilage in immunodeficient rats. Herein, we induced iMSCs through neural crest cell intermediates. For the relevance to prospective future clinical application, induction was conducted under xeno-free/serum-free conditions. Then, clumps fabricated from an iMSC/extracellular matrix complex (C-iMSC) were transplanted into thyroid cartilage defects in immunodeficient rats. Histological examinations revealed cartilage-like regenerated tissue and human nuclear antigen (HNA)-positive surviving transplanted cells in the regenerated lesion. HNA-positive cells co-expressed SOX9, and type II collagen was identified around HNA-positive cells. These results indicated that the transplanted C-iMSCs promoted thyroid cartilage regeneration and some of the iMSCs differentiated into chondrogenic lineage cells. Induced MSCs may be a promising candidate cell therapy for human laryngotracheal reconstruction.

    DOI: 10.1016/j.scr.2021.102233

    DOI: 10.1016/j.scr.2021.102233

    PubMed

  • Ryosuke Nakamura, Tatsuya Katsuno, Yo Kishimoto, Shinji Kaba, Masayoshi Yoshimatsu, Morimasa Kitamura, Atsushi Suehiro, Nao Hiwatashi, Masaru Yamashita, Ichiro Tateya, Koichi Omori .  A novel method for live imaging of human airway cilia using wheat germ agglutinin. .  Scientific reports10 ( 1 ) 14417 - 14417   2020.9International journal

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    Multiciliated epithelial cells in the airway are essential for mucociliary clearance. Their function relies on coordinated, metachronal and directional ciliary beating, appropriate mucus secretion and airway surface hydration. However, current conventional methods for observing human airway ciliary movement require ciliated cells to be detached from airway tissues. Determining the directionality of cilia is difficult. We developed a novel method to stain airway epithelial cilia to observe their movement without releasing ciliated cells. Human tracheae were obtained from patients (n = 13) who underwent laryngectomies to treat malignancies or swallowing disorders. The tracheae were treated with fluorescently labeled wheat germ agglutinin, which interacts with the acidic mucopolysaccharides present on the cilia. Epithelial surfaces were observed using an epi-fluorescence microscope equipped with a water-immersion objective lens and a high-speed camera. Ciliary movement was observable at 125 fps (13/13 samples). Ciliated cells in close proximity mostly exhibited well-coordinated ciliary beats with similar directionalities. These findings indicated that wheat germ agglutinin renders ciliary beats visible, which is valuable for observing human airway ciliary movements in situ.

    DOI: 10.1038/s41598-020-71049-z

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    PubMed

  • Ryosuke Nakamura, Tatsuya Katsuno, Yo Kishimoto, Shinji Kaba, Masayoshi Yoshimatsu, Morimasa Kitamura, Atsushi Suehiro, Nao Hiwatashi, Masaru Yamashita, Ichiro Tateya, Koichi Omori .  A novel method for live imaging of human airway cilia using wheat germ agglutinin. .  Scientific Reports2020 ( 10 ) 14417 - 14417   2020.9A novel method for live imaging of human airway cilia using wheat germ agglutinin.Reviewed

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Masayoshi Yoshimatsu, Ryosuke Nakamura, Yo Kishimoto, Hirofumi Yurie, Yasuyuki Hayashi, Shinji Kaba, Hiroe Ohnishi, Masaru Yamashita, Ichiro Tateya, Koichi Omori .  Recurrent laryngeal nerve regeneration using a self-assembling peptide hydrogel. .  The Laryngoscope130 ( 10 ) 2420 - 2427   2019.12Recurrent laryngeal nerve regeneration using a self-assembling peptide hydrogel.Reviewed International journal

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    OBJECTIVES/HYPOTHESIS: To regenerate defected recurrent laryngeal nerves (RLNs), various methods have been developed. However, no consistently effective treatments are currently available because of their insufficient functional recovery. RADA16-I, a self-assembling peptide used clinically as a hemostat, reportedly supports neurite outgrowth and functional synapse formation in vitro. The purpose of this study was to investigate the effect of RADA16-I hydrogels on transected RLNs in rats. STUDY DESIGN: Animal experiments with controls. METHODS: Fifteen adult rats were divided into the following three groups: RADA16-I (+), RADA16-I (-), and neurectomy. A 6-mm gap of the left RLN was bridged using an 8-mm silicone tube in the RADA16-I (-) and RADA16-I (+) groups. Subsequently, RADA16-I hydrogel was injected into the tube in the RADA16-I (+) group. The surgical incisions were closed without any further treatment in the neurectomy group. After 8 weeks, laryngoscopy and electrophysiological and histological examinations were performed to evaluate the effect of RADA16-I on nerve regeneration and thyroarytenoid muscle atrophy. RESULTS: Although most rats in the three groups exhibited no improvements of their vocal fold movement, partial recovery was observed in one rat in the RADA16-I (+) group. The neurofilament-positive areas and the number of myelinated nerves in the RADA16-I (+) group were significantly higher than in the RADA16-I (-) group. The area of the left thyroarytenoid muscle in the RADA16-I (+) group was significantly larger than that of the neurectomy group. CONCLUSIONS: Our results suggested that RADA16-I hydrogel was effective for RLN regeneration. LEVEL OF EVIDENCE: NA Laryngoscope, 130:2420-2427, 2020.

    DOI: 10.1002/lary.28434

    PubMed

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MISC

Presentations

  • 吉松 誠芳   耳鼻咽喉科における基礎研究-再生医学研究- ヒトiPS細胞由来間葉系幹細胞を用いた喉頭軟骨再生研究  

    日本耳鼻咽喉科学会会報  2021.4  (一社)日本耳鼻咽喉科頭頸部外科学会

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    Language:Japanese