Updated on 2023/12/15

写真a

 
ISOBE Mari
 
Organization
Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Neurology Assistant Professor
Title
Assistant Professor
Contact information
メールアドレス
External link

Degree

  • Doctor (Rehabilitation Science) ( 2019.3   Nagoya University )

  • Master (Rehabilitation Science) ( 2016.3   Nagoya University )

  • 学士（理学療法学） ( 2014.3   北里大学 )

Research Interests

  • Sorting

  • embryo development

  • Intracellular vesicular trafficking

  • 体軸骨格筋形成

  • myoseptum

  • Clathrin adaptor

  • HiBiT

  • GGA

Research Areas

  • Life Science / Anatomy

  • Life Science / Cell biology

Education

  • Nagoya University

    - 2019.3

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    Country： Japan

  • Nagoya University   Graduate School of Medicine   Doctor of Philosophy (Rehabilitation Science), Department of Physical Therapy

    2016.4 - 2019.3

Research History

  • Kagoshima University   Graduate School of Medical and Dental Sciences, Morphological Sciences   Assistant Professor

    2019.4

  • Kagoshima University   Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Neurology   Assistant Professor

    2019.4

Professional Memberships

  • THE JAPANESE ASSOCIATION OF ANATOMISTS

  • JAPAN SOCIETY FOR CELL BIOLOGY

  • JAPAN MUSCLE SOCIETY

  • JAPANESE PHYSICAL THERAPY ASSOCIATION

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

 

Papers

  • KAMETAKA Satoshi, ISOBE Mari, KOMATA Kenshin, MORINAGA Makoto, NAGAHATA Kazuma, Lee-HOTTA Sachiko, UCHIYAMA Yasushi, SHIBATA Masahiro, SUGIURA Hideshi .  Protective effects of hachimijiogan (HJG), a Japanese Kampo medicine, on cancer cachectic muscle wasting in mice .  Biomedical Research44 ( 5 ) 199 - 207   2023.9

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    Language：English   Publisher：Biomedical Research Press  

    <p>Myogenesis is required to generate skeletal muscle tissue and to maintain skeletal muscle mass. Decreased myogenesis under various pathogenic conditions results in muscular atrophy. Through a small screening of Japanese traditional (Kampo) medicines, hachimijiogan (HJG) was shown to promote the myogenic differentiation of C2C12 myoblasts through the upregulation of myogenin. In tumor-bearing cancer-cachectic mice, HJG was also found to have a protective effect against cancer-cachectic muscle wasting. This effect was significant when HJG was administered in combination with aerobic exercise by treadmill running. Moreover, HJG ameliorated the cellular atrophy of C2C12 myotubes induced by treatment with conditioned medium derived from a colon-26 cancer cell culture. In addition, HJG suppressed H2O2-dependent myotube atrophy, suggesting that HJG could reverse the atrophic phenotypes by eliminating reactive oxygen species.</p>

    DOI： 10.2220/biomedres.44.199

    Scopus

    PubMed

  • Kametaka Satoshi, Isobe Mari, Komata Kenshin, Morinaga Makoto, Nagahata Kazuma, Lee-Hotta Sachiko, Uchiyama Yasushi, Shibata Masahiro, Sugiura Hideshi .  Protective effects of hachimijiogan(HJG), a Japanese Kampo medicine, on cancer cachectic muscle wasting in mice(タイトル和訳中) .  Biomedical Research44 ( 5 ) 199,np11 - 207,np11   2023.9Protective effects of hachimijiogan(HJG), a Japanese Kampo medicine, on cancer cachectic muscle wasting in mice(タイトル和訳中)

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    Language：English   Publisher：バイオメディカルリサーチプレス  

  • ISOBE Mari, SUZUKI Yumika, SUGIURA Hideshi, SHIBATA Masahiro, OHSAKI Yuki, KAMETAKA Satoshi .  Novel cell-based system to assay cell-cell fusion during myotube formation .  Biomedical Research43 ( 4 ) 107 - 114   2022.8

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Biomedical Research Press  

    <p>A live assay tool has been established to uncover the precise molecular mechanisms underlying complex cell fusion events in myoblasts. The novel cell-based assay, HiMy (HiBiT-based myoblast fusion), utilizes a recently developed split-luciferase technology. The assay successfully detected cell fusion in differentiating C2C12 myoblast cultures. This allowed us to measure mixing of the cytoplasm, which occurred several hours after the initiation of C2C12 differentiation. Unlike what was reported earlier, the fusion was detected a few hours after the initiation of differentiation. Thus, this assay is sensitive enough to monitor fusion events before they become detectable using conventional methods. Furthermore, a panel of laboratory compounds, including a variety of inhibitors of cellular enzymes or activities, were assayed using the HiMy assay. Lovastatin, a cholesterol biogenesis inhibitor, decreased HiMy activity by approximately 50%. In contrast, mevalonolactone, a precursor for cholesterol synthesis, increased fusion activity. These results confirmed the previous finding that the amount of cellular cholesterol positively correlates with the rate of myoblast fusion during myogenesis. These results indicate that the novel cell fusion assay is a quick, accurate, and robust method to monitor intercellular fusion events.</p>

    DOI： 10.2220/biomedres.43.107

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    PubMed

  • Isobe Mari, Suzuki Yumika, Sugiura Hideshi, Shibata Masahiro, Ohsaki Yuki, Kametaka Satoshi .  筋管形成時における細胞-細胞融合を測定する新奇細胞をベースとしたシステム(Novel cell-based system to assay cell-cell fusion during myotube formation) .  Biomedical Research43 ( 4 ) 107,np1 - 114,np1   2022.8筋管形成時における細胞-細胞融合を測定する新奇細胞をベースとしたシステム(Novel cell-based system to assay cell-cell fusion during myotube formation)

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    Language：English   Publisher：バイオメディカルリサーチプレス  

    スプリットルシフェラーゼ法を利用したHiMy(HiBiTに基づく筋芽細胞融合)によって、C2C12筋芽細胞培養における細胞融合を検出できたので報告した。これによりC2C12の分化開始の数時間後に起こる細胞質の混合を測定できた。以前に報告されていたのとは異なり、融合は分化開始の数時間後に検出された。本法は感度が高く、通常の方法で検出される以前に融合を検出できた。細胞酵素阻害剤などを含む化合物を、HiMyを用いて測定した。コレステロール合成阻害剤ロバスタチンはHiMy活性を約50%まで低下させ、コレステロール合成前駆体メバロノラクトンは融合活性を上昇させた。この新規細胞融合測定法は、細胞の融合を検出する迅速かつ正確な方法であると考えられた。

  • Miki Hieda, Taizo Matsumoto, Mari Isobe, Sadamu Kurono, Kaneko Yuka, Satoshi Kametaka, Jing-Ya Wang, Ya-Hui Chi, Kenji Kameda, Hiroshi Kimura, Nariaki Matsuura, Shuji Matsuura .  The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal. .  Scientific reports11 ( 1 ) 5358 - 5358   2021.12Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    The morphology of the Golgi complex is influenced by the cellular context, which strictly correlates with nuclear functions; however, the mechanism underlying this association remains elusive. The inner nuclear membrane SUN proteins, SUN1 and SUN2, have diverse functions together with the outer nuclear membrane nesprin proteins, which comprise the LINC complex. We found that depletion of SUN1 leads to Golgi complex dispersion with maintenance of ministacks and retained function for vesicle transport through the Golgi complex. In addition, SUN2 associates with microtubule plus-end-directed motor KIF20A, possibly via nesprin-2. KIF20A plays a role in the Golgi dispersion in conjunction with the SUN2-nesprin-2 LINC complex in SUN1-depleted cells, suggesting that SUN1 suppresses the function of the SUN2-nesprin-2 LINC complex under a steady-state condition. Further, SUN1-knockout mice, which show impaired cerebellar development and cerebellar ataxia, presented altered Golgi morphology in Purkinje cells. These findings revealed a regulation of the Golgi organization by the LINC complex.

    DOI： 10.1038/s41598-021-84750-4

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    PubMed

  • Makoto Morinaga, Naoki Sako, Mari Isobe, Sachiko Lee-Hotta, Hideshi Sugiura, Satoshi Kametaka .  Aerobic Exercise Ameliorates Cancer Cachexia-Induced Muscle Wasting through Adiponectin Signaling. .  International journal of molecular sciences22 ( 6 ) 1 - 16   2021.3Reviewed International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    Cachexia is a multifactorial syndrome characterized by muscle loss that cannot be reversed by conventional nutritional support. To uncover the molecular basis underlying the onset of cancer cachectic muscle wasting and establish an effective intervention against muscle loss, we used a cancer cachectic mouse model and examined the effects of aerobic exercise. Aerobic exercise successfully suppressed muscle atrophy and activated adiponectin signaling. Next, a cellular model for cancer cachectic muscle atrophy using C2C12 myotubes was prepared by treating myotubes with a conditioned medium from a culture of colon-26 cancer cells. Treatment of the atrophic myotubes with recombinant adiponectin was protective against the thinning of cells through the increased production of p-mTOR and suppression of LC3-II. Altogether, these findings suggest that the activation of adiponectin signaling could be part of the molecular mechanisms by which aerobic exercise ameliorates cancer cachexia-induced muscle wasting.

    DOI： 10.3390/ijms22063110

    Scopus

    PubMed

  • Isobe M, Lee S, Waguri S, Kametaka S .  Clathrin adaptor GGA1 modulated myogenesis of C2C12 myoblasts. .  PLoS One13 ( 11 ) e0207533   2018.11Clathrin adaptor GGA1 modulated myogenesis of C2C12 myoblasts.

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  • Isobe M, Lee S, Waguri S, Kametaka S .  Correction: Clathrin adaptor GGA1 modulates myogenesis of C2C12 myoblasts. .  PloS one13 ( 12 ) e0209441   2018Correction: Clathrin adaptor GGA1 modulates myogenesis of C2C12 myoblasts.

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Presentations

  • 磯部茉莉, 李佐知子, 杉浦英志, 和栗聡, 亀高諭   クラスリンアダプターGGA1による骨格筋形成制御  

    第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会合同年次大会  2019.6 

  • 磯部茉莉, 李佐知子, 和栗聡, 亀高諭   クラスリンアダプターGGA1による骨格筋分化制御機構の解析  

    第124回日本解剖学会総会・全国学術集会  2019.3 

  • 磯部茉莉, 亀高諭   HiBiTシステムを用いた筋芽細胞融合の新規検出系の開発  

    日本筋学会第4回学術集会  2018.8 

  • ○Mari Isobe, Mitsunori Fukuda, Kenshin Komata, Satoshi Kametaka   A novel cell-based assay system for monitoring the cell-cell fusion process during myotube formation  

    Tokyo 2018 Cell and Developmental Biology Meeting  2018.6 

  • 磯部茉莉, 亀高諭   クラスリンアダプターGGA1は筋管形成の成熟過程に関与する  

    第81回日本生化学会中部支部例会・シンポジウム  2017.5 

  • 磯部 茉莉, 亀高 諭   骨格筋成熟過程における細胞内分子輸送関連因子GGA1の機能  

    形態・機能  2017.8  コ・メディカル形態機能学会

  • 磯部茉莉, 亀高諭   骨格筋成熟過程における細胞内分子輸送関連因子GGA1の機能  

    コ・メディカル形態機能学会第16回学術集会  2017.9 

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    学会奨励賞受賞

  • 高垣 知輝, 磯部 茉莉, 森長 真言, 杉浦 英志, 和栗 聡, 亀高 諭   遺伝性痙性対麻痺の原因遺伝子群が構成する小胞体タンパク質ネットワーク  

    日本筋学会学術集会プログラム・抄録集  2018.8  日本筋学会

  • 磯部茉莉, 杉浦英志, 福田光則, 柴田昌宏, 亀高諭   細胞内膜交通による筋芽細胞融合の制御  

    日本筋学会第5回学術集会  2019.8 

  • 磯部 茉莉, 杉浦 英志, 福田 光則, 柴田 昌弘, 亀高 諭   細胞内膜交通による筋芽細胞融合の制御  

    日本筋学会学術集会プログラム・抄録集  2019.8  日本筋学会

  • 磯部 茉莉, 亀高 諭   筋管形成過程におけるクラスリンアダプターGGA1の分子機能  

    日本筋学会学術集会プログラム・抄録集  2017.7  日本筋学会

  • 磯部茉莉, 亀高諭   筋管形成過程におけるクラスリンアダプターGGA1の分子機能  

    第3回日本筋学会学術集会  2017.8 

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    Student's award優秀賞受賞

  • 磯部茉莉, 李佐知子, 杉浦英志, 和栗聡, 亀高諭   筋管形成におけるクラスリンアダプターGGA1の役割  

    第83回日本生化学会中部支部例会・シンポジウム  2019.5 

  • 磯部茉莉, 和栗聡, 亀高諭   筋分化過程を支える細胞内輸送システムの分子機構  

    第5回若手による骨格筋研究会  2017.11 

  • 磯部茉莉, 福田光則, 亀高諭   新規細胞融合検出系の開発と細胞融合関連因子の探索  

    第6回若手による骨格筋研究会  2018.11 

  • 磯部茉莉, 古俣謙新, 杉浦英志, 福田光則, 柴田昌宏, 亀高諭   新規細胞融合検出システムHiMy assayの開発と細胞融合関連因子の探索  

    第125回日本解剖学会総会・全国学術集会  2020.3 

  • 森長 真言, 磯部 茉莉, 迫 直輝, 李 佐知子, 杉浦 英志, 亀高 諭   有酸素運動によるがん悪液質性筋萎縮抑制効果の分子機序の解析  

    形態・機能  2021.8  コ・メディカル形態機能学会

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    Language：Japanese  

  • 亀高 諭, 高垣 知輝, 清水 貴也, 磯部 茉莉   Spg12/Reticulon2の小胞体膜局在化機構の解析  

    日本細胞生物学会大会講演要旨集  2021.6  (一社)日本細胞生物学会

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    Language：Japanese  

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Awards

  • Society Award

    2017.9   The 16th Annual Meeting of Co-Medical Research Society of Structure and Function  

    Mari Isobe

  • Outstanding Performance Award of Student's Award

    2017.8   The 3rd Annual Meeting of Japan Muscle Society  

    Mari Isobe

Research Projects

  • The mechanism of segmental breaking in the axial muscle development using a novel detection system

    Grant number：22K15358  2022.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    Mari Isobe

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    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

  • ニワトリ胚を用いた体軸骨格筋の分節性崩壊過程の形態学的解析

    Grant number：20K16110  2020.4 - 2022.3

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    磯部 茉莉

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    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

 

Teaching Experience

  • 解剖学 講義・実習

    Institution：鹿児島大学