Updated on 2023/12/05
博士（医学） ( 2000.3 東京大学 )
修士（理学） ( 1992.3 東京大学 )
Life Science / Cognitive and brain science
The University of Tokyo
- 1995.3
Country： Japan
The University of Tokyo
1986.4 - 1990.3
Country： Japan
Kagoshima University Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Functional Biology and Pharmacology Professor
2018.4
日本神経精神薬理学会
2018.9
The Japanese Society of Neuropsychopharmacology
2018.9
日本生化学会
2017.3
日本神経化学会
2005.1
Society for Neuroscience
1995.4
日本生理学会
1995.1
The Physiological Society of Japan
1995.1
日本神経科学学会
1993.6
日本分子生物学会
1990.6
Takakura M., Lam Y.H., Nakagawa R., Ng M.Y., Hu X., Bhargava P., Alia A.G., Gu Y., Wang Z., Ota T., Kimura Y., Morimoto N., Osakada F., Lee A.Y., Leung D., Miyashita T., Du J., Okuno H., Hirano Y. . Differential second messenger signaling via dopamine neurons bidirectionally regulates memory retention . Proceedings of the National Academy of Sciences of the United States of America120 ( 36 ) e2304851120 2023.9Reviewed
Roberto Pagano, Ahmad Salamian, Janusz Zielinski, Anna Beroun, Maria Nalberczak-Skóra, Edyta Skonieczna, Anna Cały, Nicole Tay, Tobias Banaschewski, Sylvane Desrivières, Antoine Grigis, Hugh Garavan, Andreas Heinz, Rüdiger Brühl, Jean-Luc Martinot, Marie-Laure Paillère Martinot, Eric Artiges, Frauke Nees, Dimitri Papadopoulos Orfanos, Luise Poustka, Sarah Hohmann, Juliane H Fröhner, Michael N Smolka, Nilakshi Vaidya, Henrik Walter, Robert Whelan, Katarzyna Kalita, Haruhiko Bito, Christian P Müller, Gunter Schumann, Hiroyuki Okuno, Kasia Radwanska . Arc controls alcohol cue relapse by a central amygdala mechanism. . Molecular psychiatry28 ( 2 ) 733 - 745 2022.11Reviewed International journal
David Mahringer, Pawel Zmarz, Hiroyuki Okuno, Haruhiko Bito, Georg B Keller . Functional correlates of immediate early gene expression in mouse visual cortex. . Peer community journal2 e45 2022.7Functional correlates of immediate early gene expression in mouse visual cortex.Reviewed International journal
Masahiro Sato, Nami Sato-Yamamoto, Ai Wakita, Misako Haraguchi, Manabu Shimonishi, Hiroyuki Okuno . Direct Injection of Recombinant AAV-Containing Solution into the Oviductal Lumen of Pregnant Mice Caused In Situ Infection of Both Preimplantation Embryos and Oviductal Epithelium. . International journal of molecular sciences23 ( 9 ) 2022.5Reviewed International journal
Misaki Niu, Atsushi Kasai, Masato Tanuma, Kaoru Seiriki, Hisato Igarashi, Takahiro Kuwaki, Kazuki Nagayasu, Keita Miyaji, Hiroki Ueno, Wataru Tanabe, Kei Seo, Rei Yokoyama, Jin Ohkubo, Yukio Ago, Misuzu Hayashida, Ken-Ichi Inoue, Masahiko Takada, Shun Yamaguchi, Takanobu Nakazawa, Shuji Kaneko, Hiroyuki Okuno, Akihiro Yamanaka, Hitoshi Hashimoto . Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses. . Science advances8 ( 11 ) eabi6375 2022.3Reviewed International journal
Mai Takakura, Reiko Nakagawa, Takeshi Ota, Yoko Kimura, Man Yung Ng, Abdalla G Alia, Hiroyuki Okuno, Yukinori Hirano . Rpd3/CoRest-mediated activity-dependent transcription regulates the flexibility in memory updating in Drosophila. . Nature communications12 ( 1 ) 628 - 628 2021.12Reviewed International journal
Attardo A, Lu J, Kawashima T, Okuno H, Fitzgerald JE, Bito H, Schnitzer MJ . Long-term consolidation of ensemble neural plasticity patterns in hippocampal area CA1 . Cell Reports25 ( 3 ) 640 - 650 2018.10Reviewed
Sami El-Boustani, Jacque P. K. Ip, Vincent Breton-Provencher, Graham W. Knott, Hiroyuki Okuno, Haruhiko Bito, Mriganka Sur . Locally coordinated synaptic plasticity of visual cortex neurons in vivo . Science360 ( 6395 ) 1349 - 1354 2018.6Reviewed
Minatohara K, Akiyoshi M, Okuno H . Role of immediate-early genes in synaptic plasticity and neuronal ensembles underlying the memory trace . Front Mol Neurosci8 78 2016.1Role of immediate-early genes in synaptic plasticity and neuronal ensembles underlying the memory traceReviewed International journal
Kawashima T, Okuno H, Bito H. . A new era for functional labeling of neurons: activity-dependent promoters have come of age . Front Neural Circuits8 37 2014.4A new era for functional labeling of neurons: activity-dependent promoters have come of ageReviewed International journal
Okuno H, Akashi K, Ishii Y, Yagishita-Kyo N, Suzuki K, Nonaka M, Kawashima T, Fujii H, Takemoto-Kimura S, Abe M, Natsume R, Chowdhury S, Sakimura K, Worley PF, Bito H. . Inverse synaptic tagging of inactive synapses via dynamic interaction of Arc/Arg3.1 with CaMKIIβ. . Cell149 ( 4 ) 886 - 898 2012.5Inverse synaptic tagging of inactive synapses via dynamic interaction of Arc/Arg3.1 with CaMKIIβ.Reviewed International journal
Tse D, Takeuchi T, Kakeyama M, Kajii Y, Okuno H, Tohyama C, Bito H, Morris RG. . Schema-dependent gene activation and memory encoding in neocortex. . Science333 ( 6044 ) 891 - 895 2011.8Schema-dependent gene activation and memory encoding in neocortex.
Okuno H. . Regulation and function of immediate-early genes in the brain: beyond neuronal activity markers. . Neuroscience Research69 ( 3 ) 175 - 186 2010.12Regulation and function of immediate-early genes in the brain: beyond neuronal activity markers.
Redondo RL, Okuno H, Spooner PA, Frenguelli BG, Bito H, Morris RG. . Synaptic tagging and capture: differential role of distinct calcium/calmodulin kinases in protein synthesis-dependent long-term potentiation. . J Neurosci30 ( 14 ) 4981 - 4989 2010.4Synaptic tagging and capture: differential role of distinct calcium/calmodulin kinases in protein synthesis-dependent long-term potentiation.
Kawashima T, Okuno H, Nonaka M, Adachi-Morishima A, Kyo N, Okamura M, Takemoto-Kimura S, Worley PF, Bito H. . Synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons. . Proc Natl Acad Sci U S A.106 ( 1 ) 316 - 321 2009.1Synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons.
Chowdhury S, Shepherd JD, Okuno H, Lyford G, Petralia RS, Plath N, Kuhl D, Huganir RL, Worley PF. . Arc/Arg3.1 interacts with the endocytic machinery to regulate AMPA receptor trafficking. . Neuron52 ( 3 ) 445 - 459 2006.11Arc/Arg3.1 interacts with the endocytic machinery to regulate AMPA receptor trafficking.
Li YX, Hashimoto T, Tokuyama W, Miyashita Y, Okuno H. . Spatiotemporal dynamics of brain-derived neurotrophic factor mRNA induction in the vestibulo-olivary network during vestibular compensation. . J Neurosci.21 ( 8 ) 2738 - 2738 2001.4Spatiotemporal dynamics of brain-derived neurotrophic factor mRNA induction in the vestibulo-olivary network during vestibular compensation.
Tokuyama W, Okuno H, Hashimoto T, Xin Li Y, Miyashita Y. . BDNF upregulation during declarative memory formation in monkey inferior temporal cortex. . Nat Neurosci.3 ( 11 ) 1134 - 1142 2000.11BDNF upregulation during declarative memory formation in monkey inferior temporal cortex.Reviewed International journal
Okuno H, Tokuyama W, Li YX, Hashimoto T, Miyashita Y. . Quantitative evaluation of neurotrophin and trk mRNA expression in visual and limbic areas along the occipito-temporo-hippocampal pathway in adult macaque monkeys. . J Comp Neurol.408 ( 3 ) 378 - 398 1999.6Quantitative evaluation of neurotrophin and trk mRNA expression in visual and limbic areas along the occipito-temporo-hippocampal pathway in adult macaque monkeys.
Okuno H, Miyashita Y. . Expression of the transcription factor Zif268 in the temporal cortex of monkeys during visual paired associate learning. . Eur J Neurosci.8 ( 10 ) 2118 - 2128 1996.10Expression of the transcription factor Zif268 in the temporal cortex of monkeys during visual paired associate learning.Reviewed International journal
Okuno H, Akahori A, Sato H, Xanthoudakis S, Curran T, Iba H. . Escape from redox regulation enhances the transforming activity of Fos. . Oncogene8 ( 3 ) 695 - 701 1993.3Escape from redox regulation enhances the transforming activity of Fos.Reviewed International journal
Itoh M, Okuno H, Yamada D, Yamashita M, Abe M, Natsume R, Kaizuka T, Sakimura K, Hoshino M, Mishina M, Wada K, Sekiguchi M, Hayashi T. . Perturbed expression pattern of the immediate early gene Arc in the dentate gyrus of GluA1 C-terminal palmitoylation-deficient mice . Neuropsychopharmacol Reports39 ( 1 ) 61 - 66 2109.3Reviewed
Roberto Pagano, Ahmad Salamian, Janusz Zielinski, Anna Beroun, Maria Nalberczak-Skóra, Edyta Skonieczna, Anna Cały, Nicole Tay, Tobias Banaschewski, Sylvane Desrivières, Antoine Grigis, Hugh Garavan, Andreas Heinz, Rüdiger Brühl, Jean-Luc Martinot, Marie-Laure Paillère Martinot, Eric Artiges, Frauke Nees, Dimitri Papadopoulos Orfanos, Luise Poustka, Sarah Hohmann, Juliane H Fröhner, Michael N Smolka, Nilakshi Vaidya, Henrik Walter, Robert Whelan, Katarzyna Kalita, Haruhiko Bito, Christian P Müller, Gunter Schumann, Hiroyuki Okuno, Kasia Radwanska . Correction: Arc controls alcohol cue relapse by a central amygdala mechanism. . Molecular psychiatry 2022.12Reviewed International journal
城山 優治, 後藤 史子, 小川 糸音, 吉田 進昭, 尾藤 晴彦, 真鍋 俊也, 奥野 浩行 . Gastrin-releasing peptideは急性ストレス下で、amygdalostriatal transition areaを通じて恐怖学習を調節する . 日本生化学会大会プログラム・講演要旨集95回 1P - 353 2022.11Gastrin-releasing peptideは急性ストレス下で、amygdalostriatal transition areaを通じて恐怖学習を調節するReviewed
Shiina Nobuyuki, Okuno Hiroyuki . Expanding evidence of fundamental regulation for biological processes by liquid–liquid phase separation . 94 ( 4 ) 483 - 484 2022.8Reviewed
Akiko Oota-Ishigaki, Keizo Takao, Daisuke Yamada, Masayuki Sekiguchi, Masayuki Itoh, Yumie Koshidata, Manabu Abe, Rie Natsume, Masaki Kaneko, Toma Adachi, Toshie Kaizuka, Nami Suzuki, Kenji Sakimura, Hiroyuki Okuno, Keiji Wada, Masayoshi Mishina, Tsuyoshi Miyakawa, Takashi Hayashi . Prolonged contextual fear memory in AMPA receptor palmitoylation-deficient mice. . Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology47 ( 12 ) 2150 - 2159 2022.5Reviewed International journal
Fumiko Goto, Yuji Kiyama, Itone Ogawa, Hiroyuki Okuno, Taeko Ichise, Hirotake Ichise, Motonobu Anai, Tatsuhiko Kodama, Nobuaki Yoshida, Haruhiko Bito, Toshiya Manabe . Gastrin-releasing peptide regulates fear learning under stressed conditions via activation of the amygdalostriatal transition area . Molecular Psychiatry27 ( 3 ) 1694 - 1703 2022Reviewed
Tetsuo Ohnishi, Yuji Kiyama, Fumiko Arima-Yoshida, Mitsutaka Kadota, Tomoe Ichikawa, Kazuyuki Yamada, Akiko Watanabe, Hisako Ohba, Kaori Tanaka, Akihiro Nakaya, Yasue Horiuchi, Yoshimi Iwayama, Manabu Toyoshima, Itone Ogawa, Chie Shimamoto-Mitsuyama, Motoko Maekawa, Shabeesh Balan, Makoto Arai, Mitsuhiro Miyashita, Kazuya Toriumi, Yayoi Nozaki, Rumi Kurokawa, Kazuhiro Suzuki, Akane Yoshikawa, Tomoko Toyota, Toshihiko Hosoya, Hiroyuki Okuno, Haruhiko Bito, Masanari Itokawa, Shigehiro Kuraku, Toshiya Manabe, Takeo Yoshikawa . Cooperation of LIM domain-binding 2 (LDB2) with EGR in the pathogenesis of schizophrenia. . EMBO molecular medicine13 ( 4 ) e12574 2021.4Reviewed International journal
Hirotaka Fujita, Ryota Oikawa, Mayu Hayakawa, Fumiaki Tomoike, Yasuaki Kimura, Hiroyuki Okuno, Yoshiki Hatashita, Carolina Fiallos Oliveros, Haruhiko Bito, Toshio Ohshima, Satoshi Tsuneda, Hiroshi Abe, Takafumi Inoue . Quantification of native mRNA dynamics in living neurons using fluorescence correlation spectroscopy and reduction-triggered fluorescent probes. . The Journal of biological chemistry295 ( 23 ) 7923 - 7940 2020.4Reviewed International journal
Koshi Murata, Tomoki Kinoshita, Yugo Fukazawa, Kenta Kobayashi, Kazuto Kobayashi, Kazunari Miyamichi, Hiroyuki Okuno, Haruhiko Bito, Yoshio Sakurai, Masahiro Yamaguchi, Kensaku Mori, Hiroyuki Manabe . GABAergic neurons in the olfactory cortex projecting to the lateral hypothalamus in mice. . Scientific reports9 ( 1 ) 7132 - 7132 2019.12Reviewed International journal
Suzuki A.Z. . A clickable caging group as a new platform for modular caged compounds with improved photochemical properties . Chemical Communications55 ( 4 ) 451 - 454 2019
Masayuki Itoh, Mariko Yamashita, Masaki Kaneko, Hiroyuki Okuno, Manabu Abe, Maya Yamazaki, Rie Natsume, Daisuke Yamada, Toshie Kaizuka, Reiko Suwa, Kenji Sakimura, Masayuki Sekiguchi, Keiji Wada, Mikio Hoshino, Masayoshi Mishina, Takashi Hayashi . Deficiency of AMPAR-Palmitoylation Aggravates Seizure Susceptibility. . The Journal of neuroscience : the official journal of the Society for Neuroscience38 ( 47 ) 10220 - 10235 2018.11Reviewed
Keietsu Kikuchi, Daisuke Ihara, Mamoru Fukuchi, Hiroki Tanabe, Yuta Ishibashi, Junya Tsujii, Masaaki Tsuda, Marisa Kaneda, Hiroyuki Sakagami, Hiroyuki Okuno, Haruhiko Bito, Yuya Yamazaki, Mitsuru Ishikawa, Akiko Tabuchi . Involvement of SRF coactivator MKL2 in BDNF-mediated activation of the synaptic activity-responsive element in the Arc gene. . Journal of neurochemistry148 ( 2 ) 204 - 218 2018.11Reviewed
Chawla MK, Gray DT, Nguyen C, Dhaliwal H, Zempare M, Okuno H, Huentelman MJ, Barnes CA. . Seizure-induced Arc mRNA expression thresholds in rat hippocampus and perirhinal cortex . Frontiers in Systems Neuroscience12 53 2018.11Seizure-induced Arc mRNA expression thresholds in rat hippocampus and perirhinal cortexReviewed
Yamada M, Suzuki Y, Nagasaki SC, Okuno H, Imayoshi I . Light Control of the Tet Gene Expression System in Mammalian Cells. . Cell reports25 ( 2 ) 487 - 500 2018.10Reviewed
Nishiguchi KM, Fujita K, Tokashiki N, Komamura H, Takemoto-Kimura S, Okuno H, Bito H, Nakazawa T . Retained Plasticity and Substantial Recovery of Rod-Mediated Visual Acuity at the Visual Cortex in Blind Adult Mice with Retinal Dystrophy. . Molecular therapy : the journal of the American Society of Gene Therapy26 ( 10 ) 2397 - 2406 2018.10Retained Plasticity and Substantial Recovery of Rod-Mediated Visual Acuity at the Visual Cortex in Blind Adult Mice with Retinal Dystrophy.Reviewed
Hiroyuki Okuno, Keiichiro Minatohara, Haruhiko Bito . Inverse synaptic tagging: An inactive synapse-specific mechanism to capture activity-induced Arc/arg3.1 and to locally regulate spatial distribution of synaptic weights. . Seminars in cell & developmental biology77 43 - 50 2018.5Inverse synaptic tagging: An inactive synapse-specific mechanism to capture activity-induced Arc/arg3.1 and to locally regulate spatial distribution of synaptic weights.Invited Reviewed
Oswald Steward, Kelly Matsudaira Yee, Shannon Farris, Patricia S. Pirbhoy, Paul Worley, Kohji Okamura, Hiroyuki Okuno, Haruhiko Bito . Delayed degradation and impaired dendritic delivery of intron-lacking EGFP-Arc/Arg3.1 mRNA in EGFP-Arc transgenic mice . Frontiers in Molecular Neuroscience10 435 - 435 2018.1Delayed degradation and impaired dendritic delivery of intron-lacking EGFP-Arc/Arg3.1 mRNA in EGFP-Arc transgenic miceReviewed
Sakiko Honjoh, Luisa de Vivo, Hiroyuki Okuno, Haruhiko Bito, Giulio Tononi, Chiara Cirelli . Higher Arc Nucleus-to-Cytoplasm Ratio during Sleep in the Superficial Layers of the Mouse Cortex . FRONTIERS IN NEURAL CIRCUITS11 60 - 60 2017.8Higher Arc Nucleus-to-Cytoplasm Ratio during Sleep in the Superficial Layers of the Mouse CortexReviewed
Jenks KR, Kim T, Pastuzyn ED, Okuno H, Taibia AV, Bito H, Bear MF, Jason SD . Arc restores juvenile plasticity in adult mouse visual cortex . Proc Natl Acad Sci USA114 9182 - 9187 2017.8Arc restores juvenile plasticity in adult mouse visual cortexReviewed International journal
Kasumi Inokuchi, Fumiaki Imamura, Haruki Takeuchi, Ryang Kim, Hiroyuki Okuno, Hirofumi Nishizumi, Haruhiko Bito, Takefumi Kikusui, Hitoshi Sakano . Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses . NATURE COMMUNICATIONS8 15977 2017.7Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses
Hirano Y, Ihara K, Masuda T, Yamamoto T, Iwata I, Takahashi A, Awata H, Nakamura N, Takakura M, Suzuki Y, Horiuchi J, Okuno H, Saitoe M . Shifting transcriptional machinery is required for long-term memory maintenance and modification in Drosophila mushroom bodies . NATURE COMMUNICATIONS7 13471 2016.11Shifting transcriptional machinery is required for long-term memory maintenance and modification in Drosophila mushroom bodiesReviewed
Fukuchi M, Nakashima F, Tabuchi A, Shimotori M, Tatsumi S, Okuno H, Bito H, Tsuda M. . Class I histone deacetylase-mediated repression of the proximal promoter of the activity-regulated cytoskeleton-associated protein gene regulates its response to brain-derived neurotrophic factor . Journal of Biological Chemistry290 6825 - 6836 2015.5Class I histone deacetylase-mediated repression of the proximal promoter of the activity-regulated cytoskeleton-associated protein gene regulates its response to brain-derived neurotrophic factor
Yamamoto K, Tanei Z, Hashimoto T, Wakabayashi T, Okuno H, Naka Y, Yizhar O, Fenno LE, Fukayama M, Bito H, Cirrito JR, Holtzman DM, Deisseroth K, Iwatsubo T . Chronic optogenetic activation augments Abeta pathology in a mouse model of Alzheimer disease . Cell Reports11 ( 6 ) 859 - 865 2015.5Chronic optogenetic activation augments Abeta pathology in a mouse model of Alzheimer diseaseReviewed International journal
Vousden DA, Epp J, Okuno H, Nieman BJ, van Eede M, Dazai J, Ragan T, Bito H, Frankland PW, Lerch JP, Henkelman RM . Whole-brain mapping of behaviourally induced neural activation in mice . Brain Struct Funct.220 ( 4 ) 2043 - 2057 2015.4Whole-brain mapping of behaviourally induced neural activation in miceReviewed International journal
Fukuchi M, Tabuchi A, Kuwana Y, Watanabe S, Inoue M, Takasaki I, Izumi H, Tanaka A, Inoue R, Mori H, Komatsu H, Takemori H, Okuno H, Bito H, Tsuda M. . Neuromodulatory effect of Gαs- or Gαq-coupled G-protein-coupled receptor on NMDA receptor selectively activates the NMDA receptor/Ca2+/calcineurin/cAMP response element-binding protein-regulated transcriptional coactivator 1 pathway to effectively induce brain-derived neurotrophic factor expression in neurons . Journal of Neuroscience35 ( 14 ) 5606 - 5624 2015.4Neuromodulatory effect of Gαs- or Gαq-coupled G-protein-coupled receptor on NMDA receptor selectively activates the NMDA receptor/Ca2+/calcineurin/cAMP response element-binding protein-regulated transcriptional coactivator 1 pathway to effectively induce brain-derived neurotrophic factor expression in neurons
Nonaka M, Kim R, Fukushima H, Sasaki K, Suzuki K, Okamura M, Ishii Y, Kawashima T, Kamijo S, Takemoto-Kimura S, Okuno H, Kida S, Bito H. . Region-specific activation of CRTC1-CREB signaling mediates long-term fear memory. . Neuron84 ( 1 ) 92 - 106 2014.10Region-specific activation of CRTC1-CREB signaling mediates long-term fear memory.
Nonaka M, Fujii H, Kim R, Kawashima T, Okuno H, Bito H . Untangling the two-way signalling route from synapses to the nucleus, and from the nucleus back to the synapses. . Philos Trans R Soc Lond B Biol Sci.369 ( 1633 ) 20130150 2013.12Untangling the two-way signalling route from synapses to the nucleus, and from the nucleus back to the synapses.Reviewed International journal
Kawashima T, Kitamura K, Suzuki K, Nonaka M, Kamijo S, Takemoto-Kimura S, Kano M, Okuno H, Ohki K, Bito H. . Functional labeling of neurons and their projections using the synthetic activity-dependent promoter E-SARE. . Nat Methods10 ( 9 ) 889 - 895 2013.7Functional labeling of neurons and their projections using the synthetic activity-dependent promoter E-SARE.
Mikuni T, Uesaka N, Okuno H, Hirai H, Deisseroth K, Bito H, Kano M. . Arc/Arg3.1 is a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum. . Neuron78 ( 6 ) 1024 - 1035 2013.6Arc/Arg3.1 is a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum.Reviewed International journal
Fujii H, Inoue M, Okuno H, Sano Y, Takemoto-Kimura S, Kitamura K, Kano M, Bito H. . Nonlinear decoding and asymmetric representation of neuronal input information by CaMKIIα and calcineurin. . Cell Reports3 ( 4 ) 978 - 987 2013.4Nonlinear decoding and asymmetric representation of neuronal input information by CaMKIIα and calcineurin.
Komatsu T, Johnsson K, Okuno H, Bito H, Inoue T, Nagano T, Urano Y. . Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method. . J Am Chem Soc133 ( 17 ) 6745 - 6751 2011.5Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method.
Sato T, Tokuyama W, Miyashita Y, Okuno H. . Temporal and spatial dissociation of expression patterns between Zif268 and c-Fos in rat inferior olive during vestibular compensation. . Neuroreport8 ( 8 ) 1891 - 1895 1997.5Temporal and spatial dissociation of expression patterns between Zif268 and c-Fos in rat inferior olive during vestibular compensation.
Okuno H, Saffen DW, Miyashita Y. . Subdivision-specific expression of ZIF268 in the hippocampal formation of the macaque monkey. . Neuroscience66 ( 4 ) 829 - 845 1995.6Subdivision-specific expression of ZIF268 in the hippocampal formation of the macaque monkey.
【「新しい分子生物学技術を用いた生体機能解析」】化学遺伝学・DREADD法と生体機能解析への応用
比嘉 なつみ, 奥野 浩行
LABIO 21 ( 89 ) 6 - 8 2023.5
Gastrin-releasing peptideはamygdalostriatal transition areaを活性化させ,ストレス負荷時の恐怖学習を調節する
後藤史子, 城山優治, 城山優治, 小川糸音, 奥野浩行, 吉田進昭, 尾藤晴彦, 真鍋俊也
日本神経化学会大会抄録集(Web) 65th 2022
Gastrin-releasing peptideは急性ストレス下で,amygdalostriatal transition areaを通じて恐怖学習を調節する
城山優治, 城山優治, 後藤史子, 小川糸音, 吉田進昭, 尾藤晴彦, 真鍋俊也, 奥野浩行, 奥野浩行
日本生化学会大会(Web) 95th 2022
湊原 圭一郎, 鈴木 穣, 菅野 純夫, 尾藤 晴彦, 奥野 浩行 認知活動により活性化される前初期遺伝子Arc発現海馬神経細胞におけるスパイン形態解析およびトランスクリプトーム解析
日本生化学会大会プログラム・講演要旨集 2018.9 (公社)日本生化学会
中原 真由美, 向原 桂香, 大江 将軍, 城山 優治, 奥野 浩行, 上村 裕一 術後認知機能障害に関与する大脳神経回路同定の試み
日本集中治療医学会雑誌 2020.9 (一社)日本集中治療医学会
奥野 浩行, 荒木 杏菜, 湊原 圭一郎 若手研究者が取り組む脳科学研究の最新技術と創薬応用 シナプス活動依存的遺伝子発現に基づく記憶関連細胞標識法の開発と応用
日本薬学会年会要旨集 2017.3 (公社)日本薬学会
田渕 明子, 福地 守, 菊池 啓悦, 久保 友喜美, 庄司 しずく, 袴田 知之, 田中 拓郎, 佐藤 夏美, 石橋 悠太, 金田 真理彩, 阪上 洋行, 尾藤 晴彦, 奥野 浩行, 大塚 稔久, 津田 正明 細胞骨格により制御される転写と細胞機能:細胞形質転換と疾患 神経細胞におけるSRFコファクターMKL(MRTF)による遺伝子発現制御と形態制御
生命科学系学会合同年次大会 2017.12 生命科学系学会合同年次大会運営事務局
金子 雅規, 伊藤 政之, 山下 真梨子, 奥野 浩行, 阿部 学, 山崎 真弥, 夏目 里恵, 貝塚 利恵, 崎村 建司, 星野 幹雄, 三品 昌美, 林 崇 大脳異常興奮の抑制に関わるAMPA受容体パルミトイル化依存的なシナプス機能制御
日本生物学的精神医学会・日本神経精神薬理学会合同年会プログラム・抄録集 2017.9 日本生物学的精神医学会・日本神経精神薬理学会
城山 優治, 中原 真由美, 大江 将軍, 坂口 茜, 黒江 那彩, 上村 裕一, 奥野 浩行 マウスにおける全身麻酔下手術による扁桃体の神経活動亢進と記憶障害
日本生化学会大会プログラム・講演要旨集 2020.9 (公社)日本生化学会
Itoh Masayuki, Yamashita Mariko, Okuno Hiroyuki, Abe Manabu, Yamazaki Maya, Natsume Rie, Kaizuka Toshie, Sakimura Kenji, Hoshino Mikio, Mishina Masayoshi, Hayashi Takashi パルミトイル化によるAMPA型グルタミン受容体の制御は発作感受性を調節する(Palmitoylation-dependent regulation of the AMPA receptor modulates seizure susceptibility)
Journal of Pharmacological Sciences 2017.3 (公社)日本薬理学会
Okuno Hiroyuki, Ishii Yuichiro, Endo Toshihiro, Minatohara Keiichiro, Bito Haruhiko シナプス生理学の解明 シナプス機能の最先端研究 ArcによるAMPA受容体の表面動力学および認知処理の調節(Illumination of Synapse Physiology: Frontier researches of synaptic functions Regulation of surface dynamics of AMPA receptors and cognitive processes by Arc)
The Journal of Physiological Sciences 2018.3 (一社)日本生理学会
Ishii Yuichiro, Okuno Hiroyuki, Fujii Hajime, Bito Haruhiko グルタミン酸シナプスの分子・細胞神経生物学 表面グルタミン酸受容体発現の長期増強の単一シナプスイメージング(Molecular and cellular neurobiology of glutamatergic synapse Imaging long-term potentiation of surface glutamate receptors at single synapses)
The Journal of Physiological Sciences 2017.3 (一社)日本生理学会
城山 優治, 奥野 浩行, 内藤 眞, 尾藤 晴彦, 児玉 龍彦, 真鍋 俊也, 穴井 元暢 てんかん発症に至るまでの海馬歯状回 APE欠損をモデルとして
日本生化学会大会プログラム・講演要旨集 2019.9 (公社)日本生化学会
Bito Haruhiko, Ishii Yuichiro, Okuno Hiroyuki Synaptic tagging and capture hypothesisに対する新しい分子的洞察 逆synaptic tagging 活動誘発性Arcを捕捉するために不活性シナプスを標的とするメカニズム(New molecular insights into the synaptic tagging and capture hypothesis Inverse synaptic tagging: an inactive synapse-targeted mechanism to capture activity-induced Arc)
The Journal of Physiological Sciences 2019.6 (一社)日本生理学会
Okuno Hiroyuki, Araki Anna, Minatohara Keiichiro, Bito Haruhiko, Imayoshi Itaru Arc-based functional labelingにより明らかとなったマウス海馬の遠隔記憶痕跡(Remote memory traces in the mouse hippocampus revealed by Arc-based functional labeling)
The Journal of Physiological Sciences 2019.6 (一社)日本生理学会
城山 優治, 大西 哲生, 有馬 史子, 門田 満隆, 尾藤 晴彦, 吉川 武男, 真鍋 俊也, 奥野 浩行 Ldb2 modulates synaptic function and fear learning by regulating Arc expression in the lateral nucleus of the amygdala(和訳中)
日本生化学会大会プログラム・講演要旨集 2021.11 (公社)日本生化学会
城山 優治, 後藤 史子, 小川 糸音, 吉田 進昭, 尾藤 晴彦, 真鍋 俊也, 奥野 浩行 Gastrin-releasing peptideは急性ストレス下で、amygdalostriatal transition areaを通じて恐怖学習を調節する
日本生化学会大会プログラム・講演要旨集 2022.11 (公社)日本生化学会
城山 優治, 大西 哲生, 有馬 史子, 門田 満隆, 尾藤 晴彦, 吉川 武男, 真鍋 俊也, 奥野 浩行 Ldb2は扁桃体側核におけるArcの発現を制御することでシナプス機能と恐怖条件づけ学習を制御する(Ldb2 modulates synaptic function and fear learning by regulating Arc expression in the lateral nucleus of the amygdala)
日本生化学会大会プログラム・講演要旨集 2021.11 (公社)日本生化学会
城山 優治, 大西 哲生, 有馬 史子, 門田 満隆, 尾藤 晴彦, 吉川 武男, 真鍋 俊也, 奥野 浩行 Ldb2は扁桃体側核におけるArcの発現を制御することでシナプス機能と恐怖条件づけ学習を制御する(Ldb2 modulates synaptic function and fear learning by regulating Arc expression in the lateral nucleus of the amygdala)
日本生化学会大会プログラム・講演要旨集 2021.11 (公社)日本生化学会
城山 優治, 大西 哲生, 有馬 史子, 門田 満隆, 尾藤 晴彦, 吉川 武男, 真鍋 俊也, 奥野 浩行 Ldb2は扁桃体側核におけるArcの発現を制御することでシナプス機能と恐怖条件づけ学習を制御する(Ldb2 modulates synaptic function and fear learning by regulating Arc expression in the lateral nucleus of the amygdala)
日本生化学会大会プログラム・講演要旨集 2021.11 (公社)日本生化学会
城山 優治, 大西 哲生, 有馬 史子, 門田 満隆, 尾藤 晴彦, 吉川 武男, 真鍋 俊也, 奥野 浩行 Ldb2は扁桃体側核におけるArcの発現を制御することでシナプス機能と恐怖条件づけ学習を制御する(Ldb2 modulates synaptic function and fear learning by regulating Arc expression in the lateral nucleus of the amygdala)
日本生化学会大会プログラム・講演要旨集 2021.11 (公社)日本生化学会
城山 優治, 大西 哲生, 有馬 史子, 門田 満隆, 尾藤 晴彦, 吉川 武男, 真鍋 俊也, 奥野 浩行 Ldb2は扁桃体側核におけるArcの発現を制御することでシナプス機能と恐怖条件づけ学習を制御する(Ldb2 modulates synaptic function and fear learning by regulating Arc expression in the lateral nucleus of the amygdala)
日本生化学会大会プログラム・講演要旨集 2021.11 (公社)日本生化学会
周術期免疫抑制が神経活動へ及ぼす影響と術後認知障害の関連の解明
Grant number：22K09088 2022.4 - 2025.3
日本学術振興会 科学研究費助成事業 基盤研究(C) 基盤研究(C)
長谷川 麻衣子, 内田 寛治, 奥野 浩行, 住谷 昌彦
Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）
雄マウス求愛発声と性的動機づけを担うドーパミン神経投射経路の同定
Grant number：21K03144 2021.4 - 2024.3
日本学術振興会 科学研究費助成事業 基盤研究(C) 基盤研究(C)
菅野 康太, 奥野 浩行
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
In vitro, in vivo functions and involvement in schizophrenia of lipid modifying enzyme that shows activity-dependent expression
Grant number：21K06375 2021.4 - 2024.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
Verification study and therapeutical utilization for implementation of real time monitoring in oral cancer
Grant number：21H03143 2021.4 - 2024.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)
Grant amount：\17290000 （ Direct Cost: \13300000 、 Indirect Cost：\3990000 ）
インビボ神経イメージングによる敗血症関連脳症に関与する大脳神経回路同定と治療戦略
Grant number：21K09077 2021.4 - 2024.3
日本学術振興会 科学研究費助成事業 基盤研究(C) 基盤研究(C)
中原 真由美, 新山 修平, 奥野 浩行, 城山 優治
Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）
神経応答-遺伝子発現変換を起点とした記憶痕跡形成過程の情報動態解析
Grant number：20H05068 2020.4 - 2022.3
日本学術振興会 科学研究費助成事業 新学術領域研究(研究領域提案型) 新学術領域研究(研究領域提案型)
奥野 浩行
Grant amount：\5720000 （ Direct Cost: \4400000 、 Indirect Cost：\1320000 ）
学習時に前初期遺伝子発現を伴う活性化神経細胞は記憶痕跡（エングラム）として機能する。しかしながら、記憶獲得時の神経活動が前初期遺伝子現を誘起する機構は不明であり、さらには、前初期遺伝子発現が記憶痕跡の性質獲得に寄与する分子機構についてもほとんど理解が進んでいない。これらの知見を得るため、本研究では学習時や記憶想起時の神経応答パターンとそれに続く前初期遺伝子発現をマウスを用いて同一個体で連続的に解析する。この目的のため、本年度はまず緑色光励起および赤色蛍光の画像取得を可能とする頭部搭載型顕微鏡の作成を行った。作成された顕微鏡は感度面では緑色蛍光取得システムには及ばないものの前初期遺伝子発現レポーターを観察するためには十分な性能を有した。また、緑色蛍光の遺伝子発現レポーターと組み合わせて使用できるように赤色蛍光のカルシウムセンサー(XCaMP-R)を研究室に導入し、これを神経細胞特異的プロモーター（CaMK2プロモーターやSynapsinIプロモーター）下で発現するコンストラクトを作成し、アデノ随伴ウイルス(AAV)ベクターにパッケージングした。同様に新たな前初期遺伝子発現レポーターを構築し、AAVベクターにパッケージング、精製を行った。さらに、前初期遺伝子発現に続く後期遺伝子発現の網羅的転写産物解析を行うにあたり、これまで未解析の時点データを得るため新たに可塑性誘発刺激後の様々なタイミングで海馬歯状回顆粒細胞を採取し、そこからRNA-Seqライブラリーを作成した。
オプトジェネティクスをもちいた麻酔薬による術後認知障害の機序の解明
Grant number：19K09355 2019.4 - 2022.3
日本学術振興会 科学研究費助成事業 基盤研究(C) 基盤研究(C)
向原 桂香, 長谷川 麻衣子, 奥野 浩行
Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）
神経活動レポーター遺伝子の脳内注入:定位脳手術により、前頭前野、海馬、扁桃体に注入することや、レンズプローブ植込み神経活動レポーターの発現評価:前頭前野および海馬、または偏桃体上にグリンレンズプローブを植込み、神経活動レポーターの局在と発現量を小型統合蛍光顕微鏡をもちいて評価することの実験系作成をおこなった。全身麻酔下手術侵襲中の神経活動評価:2%イソフルラン± NMDA antagonist (MK801 0.05 mg/kg i.p.)投与下に術後痛モデルを作成した。切開前後の前頭前野、海馬における神経活動による細胞内カルシウム応答GCaMP6f、また、活動依存的な遺伝子発現をArc-GFPレポーターによってリアルタイムに測定する。4術後記憶・認知行動評価と神経活動の相関:術前に学習をおこない、術後にマウスに音を聴かせ条件刺激 (音)のみですくみ反応が出現するか評価し、恐怖記憶への麻酔下手術侵襲の影響を明らかにする。
5脳マトリックス間の相互作用:前頭前野―海馬―扁桃体の連関はArc-Pro-Venus-Pestトランスジェニックマウスをもちいて検証した。手術前後のArc依存性Venus発現神経細胞を免疫組織染色によって評価した。オプトジェネティクスをもちいた術中Arc+neuron操作と術後恐怖関連痛み行動の検討:全身麻酔中にArc-ChR2あるいはArch-T-Arc neuronをそれぞれ青色光、緑色光を当てて活性化もしくは抑制し、この影響を条件付け恐怖行動実験によって評価する過程にある。
機能的回路多重標識と転写産物プロファイリングによる脳多領野記憶痕跡モデルの検証
2018 - 2019
文部科学省 新学術領域研究(研究領域提案型)
奥野 浩行
シナプス可塑性関連因子Arcによる認知機能調節機構の解明
2015 - 2018
文部科学省 科学研究費補助金(基盤研究(B))
奥野 浩行
