Updated on 2022/07/26

写真a

 
TAKASHI Yuko
 
Organization
Research Field in Dentistry, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Oral and Maxillofacial Rehabilitation Assistant Professor
Title
Assistant Professor

Degree

  • 博士(歯学) ( 2021.2   鹿児島大学 )

  • 学士(歯学) ( 2000.3   鹿児島大学 )

Research Areas

  • Life Science / Conservative dentistry

Education

  • Kagoshima University

    - 2021.2

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    Country: Japan

Research History

  • Kagoshima University   Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Oral and Maxillofacial Rehabilitation   Assistant Professor

    2020.1

  • Kagoshima University   Medical and Dental Hospital, Medical and Dental Sciences Area Medical and Dental Hospital Clinical Center Advanced Dentistry Center   Assistant Professor

    2017.10 - 2019.12

 

Papers

  • NISHITANI Tomiko, TATSUYAMA Shoko, TAKASHI Yuko, KATSUMATA Aiichiro, HOSHIKA Tomohiro, NISHITANI Yoshihiro .  Dentin shear bond strengths and Releasing of calcium ion of experimental pulp capping materials .  Journal of the Japanese Association of Regenerative Dentistry19 ( 1 ) 1 - 9   2021

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    Publisher:Japanese Association of Regenerative Dentistry  

    <p>We prepared experimental pulp capping materials those were consist of Mineral Trioxide Aggregate (MTA) and a hydrophilic monomer in order to develop an adhesive pulp capping material. The purpose of this study was to investigate dentin shear bond strengths and releasing of calcium ions of those experimental or commercial pulp capping materials.</p><p>As a result, two experimental pulp capping materials had excellent marginal sealing property than commercial pulp capping agents, even higher shear bond strengths. In a comparison between experimental pulp capping agents having different composition ratios of monomer and MTA, the group with a relatively large amount of monomer showed higher shear bond strengths. The group with a large amount of MTA showed no penetration of dye in the dye penetration canal and releasing amount of calcium ion was higher than the group with a small amount of MTA. The composition ratio of MTA and a hydrophilic monomer may affect the basic property of the pulp capping material.</p><p>More research is required to determine the ideal composition ratio of MTA/monomer to develop an adhesive pulp capping material that has excellent dentin marginal sealing property and exhibits highest adhesive strength.</p>

    DOI: 10.11223/jard.19.1

  • Yuko Takashi, Kazuo Tomita, Yoshikazu Kuwahara, Mehryar Habibi Roudkenar, Amaneh Mohammadi Roushandeh, Kento Igarashi, Taisuke Nagasawa, Yoshihiro Nishitani, Tomoaki Sato .  Mitochondrial dysfunction promotes aquaporin expression that controls hydrogen peroxide permeability and ferroptosis .  Free Radical Biology and Medicine161   60 - 70   2020.12

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    Publisher:Free Radical Biology and Medicine  

    DOI: 10.1016/j.freeradbiomed.2020.09.027

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  • Nagasawa Taisuke, Nishiyama Nobuyoshi, Kunimasa Akihiro, Sato Tomoaki, Tomita Kazuo, Kuwahara Yoshikazu, Igarashi Kento, Takashi Yuko, Tanaka Koh-ichi, Kitanaka Junichi, Kitanaka Nobue, Takemura Motohiko .  Analysis of hydrogen peroxide resistance mechanism on hydrogen peroxide resistant cancer cells. .  Proceedings for Annual Meeting of The Japanese Pharmacological Society92 ( 0 ) 2 - P-122   2019

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    Publisher:Japanese Pharmacological Society  

    <p>&lt;purpose&gt;</p><p>Hydrogen peroxide is known as one of ROS which gives oxidative stress to cells and induces apoptosis. However, details of the mechanism for cancer cells by hydrogen peroxide is still unknown.We have established "hydrogen peroxide resistant (HR) cancer cells" that are resistant to high concentration hydrogen peroxide. The mechanism of resistance to hydrogen peroxide acquired by HR cancer cells has not yet been elucidated, however if the mechanism becomes clear, it could be applied to cancer treatment. In this study, therefore, we aimed to elucidate its hydrogen peroxide resistance mechanism and carried out the following experiment.</p><p>&lt;method&gt;</p><p>Cell lines that continued to survive against graded hydrogen peroxide treatment of HeLa (up to 70 μM) and SAS (up to 35 μM) were subjected to hydrogen peroxide at the concentrations of 0, 25, 50, 75 and 100 μM respectively, and the cell viability was examined by WST assay. Subsequently, the endogenous catalase enzymatic activity of HR cancer cells was measured using Catalase Assey Kit (SIGMA).Furthermore, lipid peroxidation of HR cancer cells was analyzed by immunofluorescence using 4-hydroxynonenal (HNE) and 5-lipoxygenase (5-LOX) antibody. HNE is typical lipid peroxidation marker and 5-LOX is known as lipid peroxidase. </p><p>&lt;results and discussion&gt;</p><p>In HeLa and SAS parental cells, they survived to the extent of 25 μM by hydrogen peroxide treatment. On the other hand, stepwise hydrogen peroxide-treated cells survived up to100 μM (HeLa) and 50 μM (SAS), showingresistance to hydrogen peroxide. Analysis of catalase enzyme activity showed significant increase in HeLa HR cells compared with the HeLa parent, but there were no significant differences in SAS cells.</p><p>Furthermore, HNE and 5-LOX expression levels in HR cells were significantly decreased compared with the parental cells byimmunofluorescent staining. </p><p>As mentioned above, the membrane lipid peroxidation is regulated by the expression of 5-LOXrather than a catalase activity. The decreased 5-LOX expression may suppress lipids peroxidation in plasma membrane and leadto retention viability to hydrogen peroxide in HR cells.</p>

    DOI: 10.1254/jpssuppl.92.0_2-P-122

  • Tomohiro HOSHIKA, Hiromasa HAYASHI, Masahiro NAGAYAMA, Shoko NAGAYAMA, Yoshihiro NISHITANI, Aiichiro KATSUMATA, Chun-Chan TING, Tomiko NISHITANI, Yuko TAKASHI, Tamaki KATSUMATA, Akira NEMOTO, Masayuki ITO, Tomoyuki IWATA .  Effects of a New Pulp-capping Material on Cell Proliferation and Shear Bond Strength to Dentin .  The Japanese Journal of Conservative Dentistry62 ( 4 ) 208 - 214   2019

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    Publisher:The Japanese Society of Conservative Dentistry  

    <p> Purpose: Calcium hydroxide preparations and mineral trioxide aggregate (MTA) are used as pulp-capping materials. However, these materials do not adhere to teeth and dental restorations. The purpose of this study was to develop a new adhesive pulp-capping material.</p><p> Methods: In this study, the new pulp-capping material (PCX-TBB, Sun Medical, Tokyo, Japan) combined with 2-hydroxypropyl methacrylate and Portland cement, TheraCal LC (BISCO Dental Products, Schaumburg, IL, USA) and Dycal (Dentsply Sirona, York, PA, USA) were used. Discs of each material were immersed in distilled water, and the pH and calcium ion concentration were measured while exchanging water every 24, 48 and 72 hours. Next, human dental pulp-derived cells (DPCs: Dental Pulp Cells, AD010-F-RA, DV Biologics) were cultured with the discs in a 24-well plate. Then, the number of cells was measured after 24, 48 and 72 hours of culture. In addition, the dentin surfaces of 30 fresh bovine mandibular anterior teeth were used as the adhesion surface, and half of the dentin specimens were used as demineralized dentin. We cured each pulp-capping material on dentin, then performed shear bond strength tests on the bonded specimens.</p><p> Results: The pH was over 10.5 in distilled water for all the pulp-capping materials. In addition, PCX-TBB and TheraCal LC showed significantly higher cumulative calcium ion release values than Dycal at all times, and after 72 hours PCX-TBB showed the highest value. As for the effect on cell proliferation, each pulp-capping material group showed a significantly lower cell number than the control group; the PCX-TBB group was second after the control group at 72 hours, and the cell numbers of the TheraCal LC and Dycal groups were significantly lower.</p><p> PCX-TBB showed significantly higher shear bond strength than other materials under normal and demineralized dentin conditions. In addition, PCX-TBB and TheraCal LC showed significantly higher values for demineralized dentin than normal dentin. On the other hand, Dycal showed lower values for normal dentin and demineralized dentin.</p><p> Conclusions: In this study, PCX-TBB had a pH as high as that of currently marketed pulp-capping materials, had a sustained release of calcium greater than that of current products, and exhibited adhesion to teeth. PCX-TBB was shown to be an adhesive pulp-capping material.</p>

    DOI: 10.11471/shikahozon.62.208

  • Tomita Kazuo, Takemura Motohiko, Nishiyama Nobuyoshi, Fukumoto Manabu, Nishitani Yoshihiro, Kurimasa Akihiro, Sato Tomoaki, Kuwahara Yoshikazu, Igarashi Kento, Takashi Yuko, Nagasawa Taisuke, Nabika Hideki, Tanaka Koh-ichi, Kitaknaka Junichi, Kitanaka Nobue .  Involvement of ALOX in resistance or sensitivity of cancer treatment via plasma membrane oxidation state. .  Proceedings for Annual Meeting of The Japanese Pharmacological Society92 ( 0 ) 2 - O-12   2019

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    <p>Although radiation therapy is one of the choices to treat cancers and is an excellent local treatment method, existence of radiation resistant cell is a major problem. We established clinically relevant radioresistant (CRR) cells that can survive exposing to 2 Gy/day X-rays for more than 30 days. However, the mechanism to obtain resistance has not been elucidated yet. We investigated the relationships between resistant mechanism to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and plasma membrane state in CRR cells. </p><p> CRR cells showed resistance to H<sub>2</sub>O<sub>2</sub>, but catalase enzyme activity was down-regulated. Plasma membrane potential were low, no internal H<sub>2</sub>O<sub>2 </sub>increase and no lipid peroxidation were seen even after 2 hours of H<sub>2</sub>O<sub>2 </sub>treatment in CRR cells. Administration of oxidized lipid led to further cell death after H<sub>2</sub>O<sub>2</sub> treatment in CRR cells. The lipoxygenase (ALOX) gene and protein expressions were down-regulated in CRR cells. We also established stress-sensitive ρ<sup>0</sup> cells that lack mitochondrial DNA. Gene and protein expressions of ALOX were up-regulated in ρ<sup>0</sup> cells. Expression of cyclooxygenase-2 does not seem to be involved in this mechanism. </p><p> These results suggest that the involvement of ALOX in resistance or sensitivity of cancer treatment.</p>

    DOI: 10.1254/jpssuppl.92.0_2-O-12

  • Takashi Y, Tomita K, Kuwahara Y, Nabika H, Igarashi K, Nagasawa T, Kurimasa A, Fukumoto M, Nishitani Y, Sato T .  Data on the aquaporin gene expression differences among ρ<sup>0</sup>, clinically relevant radioresistant, and the parental cells of human cervical cancer and human tongue squamous cell carcinoma. .  Data in brief20   402 - 410   2018.10Data on the aquaporin gene expression differences among ρ<sup>0</sup>, clinically relevant radioresistant, and the parental cells of human cervical cancer and human tongue squamous cell carcinoma.

  • Tomita K. .  Clinically relevant radioresistant cells exhibit resistance to H<inf>2</inf>O<inf>2</inf> by decreasing internal H<inf>2</inf>O<inf>2</inf> and lipid peroxidation .  Tumor Biology40 ( 9 ) 1010428318799250   2018.9

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    Publisher:Tumor Biology  

    DOI: 10.1177/1010428318799250

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  • Takashi Yuko, Nishiyama Nobuyoshi, Miyawaki Shouichi, Nishitani Yoshihiro, Sato Tomoaki, Tomita Kazuo, Kuwahara Yoshikazu, Yamanishi Sayuri, Nabika Hideki, Tanaka Koh-ich, Kitanaka Junichi, Kitanaka Nobue, Takemura Motohiko .  Sensitivity for hydrogen peroxide in ρ<sup>0</sup> cells and involvement of membrane status .  日本薬理学会年会要旨集2018 ( 0 ) PO4 - 6-14   2018

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    Publisher:公益社団法人 日本薬理学会  

    <p>Mitochondria have been recognized as ¨Powerhouse of the Cell¨ for a long time. Lately, mitochondria have also been shown as the primary site of reactive oxygen species (ROS) generation and to be involved in apoptosis. Mitochondria have its own DNA (mtDNA) and mtDNA encodes 13 mitochondrial structure protein.It has been shown that mtDNA damage by ROS causes various types of diseases such as cancer. Therefore, these damaged cells will provide valuable cell models to study oxidative stress and overcome ROS derived diseases. We established mtDNA depleted ρ<sup>0</sup> cells from HeLa and SAS cell lines and investigated the effect of ROS, especially hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) sensitivity. First, we performed H<sub>2</sub>O<sub>2</sub> treatment on HeLa and SAS ρ<sup>0</sup> cells at a concentration of 0 to 100 μM. Next, we investigated catalase gene expression and catalase enzyme activity. As a result, ρ<sup>0</sup> cells showed high sensitivity to H<sub>2</sub>O<sub>2</sub> compared with its parental cells, even though the catalase activity of ρ<sup>0</sup> cells were up-regulated. We investigated cell membrane potential by DiBAC4 (3), lipid peroxidation by HNE immunostaining, endogenous H<sub>2</sub>O<sub>2</sub> amount by HYDROP and uptake of H<sub>2</sub>O<sub>2</sub> by stable isotope-containing H<sub>2</sub>O<sub>2</sub>. The result showed that the membrane potential of ρ<sup>0</sup> cells was lower than their parental cells, the amount of HNE was elevated in ρ<sup>0</sup> cells as compared with their parental cells, internal H<sub>2</sub>O<sub>2</sub> amount was significantly increased only in ρ<sup>0</sup> cells after 50 μM H<sub>2</sub>O<sub>2</sub> treatment for 1 h and uptake of H<sub>2</sub>O<sub>2</sub> were increased in ρ<sup>0</sup> cells as compared with their parental cells. These changes in the membrane state, particularly lipid peroxidation, occurred in ρ<sup>0</sup> cells as compared with their parental cells, leads to increase membrane permeability of H<sub>2</sub>O<sub>2</sub>. And the timing of the intracellular H<sub>2</sub>O<sub>2</sub> concentration will be advanced. In conclusion, ρ<sup>0</sup> cells are considered to be more susceptible to H<sub>2</sub>O<sub>2</sub> than their parental cells because the membrane statuses change.</p>

    DOI: 10.1254/jpssuppl.WCP2018.0_PO4-6-14

  • Furukawa Minami, Nishiyama Nobuyoshi, Nishitani Yoshihiro, Miyawaki Shouichi, Sato Tomoaki, Tsukahara Takao, Yamanishi Sayuri, Tomita Kazuo, Takashi Yuko, Tanaka Koh-ich, Kitanaka Junichi, Kitanaka Nobue, Takemura Motohiko .  Neonatal maternal separation delays the GABA excitatory-to-inhibitory functional switch by inhibiting KCC2 expression and induces developmental disorders-like behaviors in mice .  日本薬理学会年会要旨集2018 ( 0 ) PO3 - 1-49   2018

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    <p>Early life stress leads to neurodevelopmental disorders. The excitatory-to-inhibitory functional switch of γ-aminobutyric acid (GABA switch) normally occurs on the up-regulation of K<sup>+</sup>-Cl<sup>-</sup> cotransporter-2 (KCC2) in the first to the second postnatal week. GABA switch is necessary for normal construction of neural circuit. Therefore, early life stress may induce behavioral abnormalities through the influence of GABA switch. </p><p> The purpose of this study is to examine the effect of maternal separation (MS) on the timing of GABA switch and on the adolescent behavior. </p><p> Neonatal C57BL/6J male mice were divided into control group (Ctl) and experimental group. Experimental (MS) group was subjected to maternal separation for 3 hours a day during postnatal day 1 (P1) to P21. Then both groups were weaned at P22. We conducted quantification of KCC2 immunoreactivity (IR) at P7, 14, 21 and 35, and analyzed the timing of GABA switch by Ca<sup>2+</sup> imaging method during P2 to P15. Behavioral analyzes such as open field, novel object recognition, elevated cross maze, attack action tests, were conducted from P35 to P38. MS resulted in a decrease in perimembrane KCC2-IR in the CA1 and CA3 regions at P7, 14, 21 and 35. NKCC1-IR in CA1 and CA3 were no significant difference between the Ctl and MS groups. Next, we analyzed the timing of GABA switch by Ca<sup>2+</sup> imaging method. 10 μM of muscimol induced Ca<sup>2+</sup> responses present in a higher percentage of neurons in the MS than in the Ctl on P8 and P11. The Ca<sup>2+</sup> responses induced by muscimol were completely inhibited by pre-treatment with 10 μM of bicuculline. Compared to mice in the Ctl group on P35, MS group had decreased amounts of perimembrane KCC2 in hippocampal CA1 and CA3. There were no significant differences in NKCC1 in both groups on P35. We also found cognition and attention defects in MS group by behavioral analyzes. </p><p> Taken together, neonatal MS inhibited the expression of perimembrane KCC2 for a long lasting period, leading to the delay timing of GABA switch in the hippocampus. The disrupted KCC2 expression could be associated with abnormal behaviors during adolescence.</p>

    DOI: 10.1254/jpssuppl.WCP2018.0_PO3-1-49

  • Tomita Kazuo, Nishiyama Nobuyoshi, Miyawaki Shouichi, Fukumoto Manabu, Nishitani Yoshihiro, Kurimasa Akihiro, Sato Tomoaki, Kuwahara Yoshikazu, Takashi Yuko, Yamanishi Sayuri, Nabika Hideki, Tanaka Koh-ich, Kitanaka Junichi, Kitanaka Nobue, Takemura Motohiko .  Analysis of oxidative stress response in clinically relevant radioresistant cells .  日本薬理学会年会要旨集2018 ( 0 ) PO3 - 7-32   2018

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    <p>Radiation therapy is one of the choices to treat malignant tumors. Although radiation therapy has been established as an excellent local treatment for malignant tumors, existence of radiation resistant cell is a major problem to overcome. To reveal radioresistant mechanism, we investigated using clinically relevant radioresistant (CRR) cells that had been obtained by exposing to 2 Gy/day X-rays for more than 30 days. CRR cells show not only radioresistance but also resistant to anticancer drug docetaxel. CRR cells also show low frequency of DNA double strand breaks, low mitochondrial membrane potential and produce little amount of reactive oxygen species (ROS). However, the molecular mechanism to obtain radio-resistant is not clear. So, we try to reveal the resistant ability to other oxidative stress such as hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). First, we investigated the resistance of CRR cells against H<sub>2</sub>O<sub>2</sub> using WST assay. As a result, these cells showed resistant to H<sub>2</sub>O<sub>2</sub>. Next, we examined gene expression and enzyme activity of catalase that is H<sub>2</sub>O<sub>2</sub> catabolic enzyme. Catalase expression was up-regulated in CRR cells. However, catalase enzyme activity was down-regulated. We also investigated mitochondrial DNA (mtDNA) copy number. It was shown that the mtDNA copy number was decreased in CRR cells. In addition, the amount of ATP, ATPase gene expressions and plasma membrane potential were investigated in CRR cells. It was shown that the amount of ATP decreased, ATPase gene expressions were up regulated and plasma membrane potential were low in CRR cells. Furthermore, increase of internal H<sub>2</sub>O<sub>2</sub> amount and lipid peroxidation was investigated. As a result, increase of internal H<sub>2</sub>O<sub>2</sub> amount and lipid peroxidation were seen in parental cells 2h after H<sub>2</sub>O<sub>2</sub> administration. On the other hand, increase of internal H<sub>2</sub>O<sub>2</sub> amount and lipid peroxidation were not seen in CRR cells 2h after H<sub>2</sub>O<sub>2</sub> administration. These results suggest that the decrease of cell response through plasma membrane component is the main factor rather than internal oxidative stress scavenging enzyme activity to obtain resistance against oxidative stress in CRR cells.</p>

    DOI: 10.1254/jpssuppl.WCP2018.0_PO3-7-32

  • Tomita K. .  Sensitivity of mitochondrial DNA depleted ρ0 cells to H<inf>2</inf>O<inf>2</inf> depends on the plasma membrane status .  Biochemical and Biophysical Research Communications490 ( 2 ) 330 - 335   2017.8

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    Publisher:Biochemical and Biophysical Research Communications  

    DOI: 10.1016/j.bbrc.2017.06.044

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  • Tatsuyama Shoko, Takashi Yuko, Katsumata Aiichiro, Kajihara Takehiro, Hoshika Tomohiro, Imai Koichi, Nishitani Yoshihiro .  Effect of Cell Growth on Adhesive Root Canal Sealer Containing Portlamd Cement .  Journal of the Japanese Association of Regenerative Dentistry15 ( 1 ) 3 - 9   2017

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    Publisher:Japanese Association of Regenerative Dentistry  

    <p>It is advantageous to use adhesive root canal sealer for prevention of reinfection in pulpectomy. Mineral trioxide aggregate (MTA) is a hydraulic cement with bactericidal action and hard tissue induction ability and it has been widely used in dental treatment. The purpose of this study was to examine the effect of cell growth on adhesive root canal sealer containing white portland cement (WPC) for development of new adhesive root canal sealer with efficacy of MTA. There was no change in the number of cells in which human periodontal ligament cells survived in the culture medium containing components eluted from the experimental sealer in case of low concentration. The pH of the culture medium and the concentration of Calcium dissolved in the culture medium were WPC concentration-dependent increase. More research is required to determine if MTA-containing adhesive root canal sealer will induce hard tissue at root apex.</p>

    DOI: 10.11223/jard.15.3

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MISC

  • 細胞膜鉄動態と酸化ストレス抵抗性 Reviewed

    富田 和男,桑原 義和, 五十嵐 健人, 高 裕子, 長澤 大成, 山西 沙祐里, 西谷 佳浩, 漆原 佑介,宮脇 正一, 栗政 明弘,福本 学, 佐藤 友昭

    東北医科薬科大学研究誌   2019.12

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)  

  • ミトコンドリア障害細胞における酸化ストレス感受性と細胞膜動態 Reviewed

    富田 和男,桑原 義和, 高 裕子, 並河 英紀, 西谷 佳浩, 漆原 佑介, 山西 沙祐里, 古川 みなみ、宮脇 正一, 栗政 明弘,福本 学, 佐藤 友昭

    東北医科薬科大学研究誌   2017.11

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Presentations

  • 髙 裕子, 達山祥子, 富田和男, 星加知宏, 佐藤友昭, 西谷佳浩   歯髄細胞へのストレスはアクアポリン9の発現を低下させる  

    第156回日本歯科保存学会学術大会  2022.6 

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    Event date: 2022.6 - 2022.7

    Language:Japanese   Presentation type:Poster presentation  

    Venue:郡山(WEB開催)   Country:Japan  

  • 髙 裕子, 宮下桂子, 達山祥子, 星加知宏, 西谷佳浩   ストレス誘導性細胞死に対するアクアポリンの関与  

    第152回日本歯科保存学会学術大会  2020.6 

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    Event date: 2020.6

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神戸 (誌上開催)  

  • 高 裕子   酸化ストレス誘導性がん細胞死はミトコンドリアによるアクアポリン発現制御を介したフェロトーシスである  

    第13回鹿児島大学大学院医歯学総合研究科口腔先端科学教育研究センター歯系研究発表会  2020.12  鹿児島大学大学院医歯学総合研究科口腔先端科学教育研究センター

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島   

  • 富田 和男, 桑原 義和, 鳥居 征司, 五十嵐 健人, 髙 裕子, 長澤 大成, 田中 康一, 北中 純一, 北中 順惠, 栗政 明弘, 西谷 佳浩, 西山 信好, 竹村 基彦, 佐藤 友昭   ALOXによる酸化ストレス抵抗性制御  

    第73回日本薬理学会西南部会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:熊本 (WEB開催)  

  • Kazuo Tomita, Yuko Takashi, Kento Igarashi, Yoshihiro Nishitani, Tomoaki Sato   Mitochondrial dysfunction enhances hydrogen peroxide-induced ferroptosis by up-regulating the expression of aquaporin3, 5, and 8  

    第62回歯科基礎医学会学術大会 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:鹿児島 (WEB開催)  

  • 富田 和男, 桑原 義和,五十嵐健人, 髙 裕子, 長澤 大成, 山西 沙祐里, 田中 康一, 北中 純一, 北中 順惠, 栗政 明弘, 宮脇 正一, 西谷 佳浩, 西山 信好, 竹村 基彦, 福本 学, 佐藤 友昭   CRR細胞の酸化ストレス抵抗性に対する細胞内遊離鉄の関与  

    第93回日本薬理学会年会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪 (誌上開催)  

  • 髙 裕子   過酸化水素処理によるがん細胞死における鉄イオンとアクアポリンの関係  

    第12回鹿児島大学大学院医歯学総合研究科口腔先端科学教育研究センター歯系研究発表会 

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    Event date: 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島  

  • 富田 和男、桑原 義和、髙 裕子、五十嵐 健人、長澤 大成、並河 英紀、田中 康一、 北中 純一、北中 順惠、栗政 明弘、西谷 佳浩、西山 信好、竹村 基彦、福本 学、 佐藤 友昭   ρ0細胞における酸化ストレス誘導死はAktシグナルを介する  

    第72回日本薬理学会西南部会 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:沖縄  

  • 髙 裕子、富田 和男、桑原 義和、五十嵐 健人、長澤 大成、田中 康一、北中 純一、北中 順惠、栗政 明弘、西谷 佳浩、西山 信好、竹村 基彦、佐藤 友昭   過酸化水素処理によるρ0細胞細胞死はAQP3と鉄イオンが関与する  

    第72回日本薬理学会西南部会 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:沖縄  

  • Kazuo Tomita, Yuko Takashi, Kent Igarashi, Yoshihiro Nishitani, Tomoaki Sato   ALOXs control lipid peroxidation and H2O2 intake in ρ0 cells  

    第61回歯科基礎医学会学術大会 

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    Event date: 2019.10

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京  

  • 富田 和男,桑原 義和,五十嵐 健人,髙 裕子,長澤 大成,山西 沙祐里,漆原 佑介,宮脇 正一,栗政 明弘,西谷 佳浩,福本 学,佐藤 友昭   miR-7-5pは細胞内Fe2+抑制を介して放射線抵抗性を制御する  

    第21回応用薬理シンポジウム 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:静岡  

  • 長澤 大成,富田 和男,桑原 義和, 五十嵐 健人,髙 裕子,佐藤 友昭   過酸化水素抵抗性がん細胞における過酸化水素抵抗メカニズムの解析  

    第92回日本薬理学会年会 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:大阪  

  • 富田 和男,桑原 義和, 五十嵐 健人,髙 裕子,長澤 大成,並河 英紀,田中 康一,北中 純一,北中 順惠,竹村 基彦, 西山 信好, 福本 学, 西谷 佳浩,栗政 明弘,佐藤 友昭   がんの治療抵抗性・感受性を制御する細胞膜酸化状態とALOXの関与  

    第92回日本薬理学会年会 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪  

  • 勝俣愛一郎,根本 章,髙 裕子,勝俣 環,西谷登美子,丁 群展,達山祥子,星加知宏,西谷佳浩   塩酸含有歯科用口腔清掃材のエナメル質表面に及ぼす影響  

    第18回美容口腔管理学会総会・学術講演会 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪  

  • 髙 裕子,富田 和男,桑原 義和,五十嵐 健人,長澤 大成,並河 英紀,田中 康一,西谷 佳浩,西山 信好,佐藤 友昭   過酸化水素処理におけるρ0細胞の膜状態変化とアクアポリン 3, 8, 9 の発現  

    第71 回日本薬理学会西南部会 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡  

  • 富田 和男,桑原 義和,髙 裕子,五十嵐 健人,長澤 大成,並河 英紀,田中 康一,北中 純一,北中 順惠,栗政 明弘,西谷 佳浩,西山 信好,竹村 基彦,福本 学, 佐藤 友昭   細胞膜酸化状態はがんの治療耐性を制御する  

    第71 回日本薬理学会西南部会 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡  

  • 星加 知宏,池田 正臣,高 裕子,西谷 登美子,勝俣 環,永山 祥子,勝俣 愛一郎,保坂 啓一,田上 順次,西谷 佳浩   ピロリドンカルボン酸を配合した新規歯磨剤の根面う蝕抑制効果に関する研究  

    第149回日本歯科保存学会学術大会 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都  

  • 勝俣 環,根本 章,髙 裕子,西谷登美子,丁 群展,永山祥子,勝俣愛一郎,星加知宏,西谷佳浩   亜鉛含有グラスアイオノマーセメントに対するStreptococcus mutans初期付着性  

    第149回日本歯科保存学会学術大会 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都  

  • Yuko Takashi, Kazuo Tomita, Kento Igarashi, Yoshihiro Nishitani, Tomoaki Sato   Sensitivity for hydrogen peroxide in ρ0 cells and involvement of membrane status  

    第60 回歯科基礎医学会学術大会 

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    Event date: 2018.9

    Language:English   Presentation type:Poster presentation  

    Venue:福岡  

  • Kazuo Tomita, Yuko Takashi, Kento Igarashi, Yoshihiro Nishitani, Tomoaki Sato   Analysis of oxidative stress response in Clinically Relevant Radioresistant cells  

    第60回歯科基礎医学会学術大会 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡  

  • 富田和男,桑原義和,髙裕子,五十嵐健人,長澤大成,山西沙祐里,大内裕也,並河英紀,田中康一,北中純一,北中順惠,漆原佑介,宮脇正一,栗政明弘,西谷佳浩,福本学,佐藤友昭   CRR細胞における過酸化水素耐性機構の解析  

    第20回応用薬理シンポジウム 

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    Event date: 2018.8

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京  

  • Yuko Takashi, Kazuo Tomita , Yoshikazu Kuwahara , Sayuri Yamanishi ,Hideki Nabika,  Koh-ichi Tanaka , Junichi Kitanaka , Nobue Kitanaka , Motohiko Takemura , Nobuyoshi Nishiyama , Shouichi Miyawaki , Yoshihiro Nishitani , Tomoaki Sato   Sensitivity for hydrogen peroxide in ρ0 cells and involvement of membrane status   International conference

    WCP2018 

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    Event date: 2018.7

    Language:English   Presentation type:Poster presentation  

    Venue:京都  

  • Minami Furukawa, Takao Tsukahara, Sayuri Yamanishi, Kazuo Tomita, Yuko Takashi, Koh-ichi Tanaka, Junichi Kitanaka, Nobue Kitanaka, Motohiko Takemura, Nobuyoshi Nishiyama, Yoshihiro Nishitani, Shouichi Miyawaki, Tomoaki Sato   Neonatal maternal separation delays the GABA excitatory-to-inhibitory functional switch by inhibiting KCC2 expression and induces developmental disorders-like behaviors in mice   International conference

    WCP2018 

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    Event date: 2018.7

    Language:English   Presentation type:Poster presentation  

    Venue:京都  

  • Kazuo Tomita , Yoshikazu Kuwahara , Yuko Takashi, Sayuri Yamanishi ,Hideki Nabika,  Koh-ichi Tanaka , Junichi Kitanaka , Nobue Kitanaka , Motohiko Takemura , Nobuyoshi Nishiyama , Shouichi Miyawaki ,Manabu Fukumoto , Yoshihiro Nishitani , Akihiro Kurimasa,Tomoaki Sato   Analysis of oxidative stress response in clinically relevant radioresistant cells   International conference

    WCP2018 

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    Event date: 2018.7

    Language:English   Presentation type:Poster presentation  

    Venue:京都  

  • 髙 裕子   CRR細胞とρ0細胞における膜状態の差異と過酸化水素に対する感受性の比較検討  

    第10回鹿児島大学大学院医歯学総合研究科口腔先端科学教育研究センター歯系研究発表会 

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    Event date: 2018.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島  

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Awards

  • 口腔先端科学優秀賞(口演発表 大学院生部門)

    2020.12   鹿児島大学大学院医歯学総合研究科口腔先端科学教育研究センター   酸化ストレス誘導性がん細胞死はミトコンドリアによるアクアポリン発現制御を介したフェロトーシスである

    高 裕子

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    Country:Japan

Research Projects

  • アクアポリンを制御するミトコンドリア移植法による新しい歯髄保存・再生療法の開 発

    2021.4 - 2024.3

    科学研究費補助金  基盤研究(C)

    髙 裕子、佐藤友昭、西谷佳浩、富田和男、達山 祥子

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    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

  • 放射線耐性は膜の状態によって規定されるか?-臨床的治療耐性細胞によるアプローチ-

    2018.4 - 2021.3

    科学研究費補助金  基盤研究(C)

    髙 裕子、桑原義和、佐藤友昭、並河英紀、西谷佳浩、富田和男

 

Teaching Experience

  • 臨床実習(保存科)

    2021.10
    Institution:鹿児島大学

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    Level:Undergraduate (specialized) 

  • 歯科保存学実習

    2021.10
    -
    2022.2
    Institution:鹿児島大学

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    Level:Undergraduate (specialized) 

  • 臨床予備実習(保存科)

    2018.4
    -
    2021.9
    Institution:鹿児島大学

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    Level:Undergraduate (specialized) 

  • 歯科保存学実習

    2017.10
    -
    2020.2
    Institution:鹿児島大学

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    Level:Undergraduate (specialized)  Country:Japan