記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ceca.2014.11.003

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.13-0512

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.13-0308

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：1-2  

DOI： 10.1016/j.vetimm.2013.09.013

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1271/bbb.130327

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.12-0496

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.12-0457

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.12-0458

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.11-0517

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.brainres.2012.08.028

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1271/bbb.120503

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1271/bbb.120379

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1271/bbb.120293

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.11-0562

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jviromet.2011.07.006

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.10-0371

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1271/bbb.100044

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記述言語：英語   出版者・発行元：Elsevier B.V.  

Experimental infection of mice with Sendai virus (SeV) is frequently used as a model of viral pathogenesis of human respiratory disease. To understand the differences in host response to SeV among mice strains, we carried out genetic mapping studies in DBA/2 (D2) (susceptible) and C57BL/6 (B6) (resistant) mice. F1, F2, and N2 backcrossed mice were generated and examined for their disease resistance and susceptibility. For the determination of virulence, percentage body weight loss and survival time were used as phenotypes. We, then, carried out a genome wide scan on 108 backcrossed mice for linkage with percentage body weight loss as phenotype. A major quantitative trait locus (QTL) showing significant linkage was mapped to the distal portion of Chr4 (SeV1). In addition, two other QTLs showing suggestive statistical linkage were also detected on Chr 8 and 14. We, further, performed genome scan for interactions with least squares analysis of variance of all pairs of informative makers in backcrossed progenies. We identified a highly significant epistatic interaction between D3Mit182 and D14Mit10, then denoted as SeV2 and SeV3, respectively, and the latter was the same locus showing a suggestive level on Chr 14 in QTL analysis. Considered genotypes of these three loci, we could account for more than 90% of genetic effect on the differential response to SeV infection between B6 and D2 mice. These findings revealed a novel gene interactions controlling SeV resistance in mice and will enable the identification of resistance genes encoded within these loci.

DOI： 10.1016/j.meegid.2009.08.011

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1271/bbb.80764

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1271/bbb.70673

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.14943/jjvr.55.4.115

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00335-007-9062-0

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1210/me.2007-0040

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00335-005-0167-z

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1089/jir.2005.25.169

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.14943/jjvr.52.4.145

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s00335-004-2424-y

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.66.1523

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER WIEN  

Monoclonal antibody E5/G6 recognized a linear epitope common to hantavirus nucleocapsid proteins. Using synthetic peptides, we identified epitope E5/G6 as the 9 merYEDVNGIRK (NP 165 - 173), in which D167, G170, I171, and R172 are indispensable. Furthermore, all the peptides synthesized using various hantavirus sequences bound MAb E5/G6 consistently, despite the existence of several amino acid variations in this region. These results indicate that MAb E5/G6 is a useful tool for detecting hantavirus antigen in rodent or patient tissues using Western blotting or other immunohistochemical assays.

DOI： 10.1007/s00705-004-0393-9

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER ASSOC LABORATORY ANIMAL SCIENCE  

The purpose of the study reported here was to define strain differences in spermatogenesis in cryptorchid testes in mice. Mice of strains AM, BALB/c, CBA/N, C3H/He, C57BL/6 (136), ddY and ICR were found to be sensitive to heat stress attributable to experimentally induced cryptorchidism. In contrast, mice of strains AKR/N (AKR), MRL/MpJ-+/+ (M+) and MRL/MpJ-lpr/lpr (lpr) were resistant to heat stress. Relative increases of apoptotic cells were detected in the sensitive group, but not in the resistant group. A decrease of proliferating cell nuclear antigen-immunoreactive cells after experimentally induced cryptorchidism was observed only in the sensitive group. These results suggested that heat stress-resistant germ cells were present in MRL and AKR strains, possibly originating from the genetic background.

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記述言語：英語  

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/S0378-1119(03)00428-1

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1292/jvms.64.1085

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1023/A:1020208802791

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記述言語：英語   出版者・発行元：COLD SPRING HARBOR LAB PRESS  

The nucleotide sequence of chicken Mx cDNA was reported earlier using the White Leghorn breed in Germany, but it showed no enhanced resistance to viruses. In this study, the nucleotide sequences of chicken Mx cDNA were determined in many breeds. A total of 25 nucleotide substitutions, of which 14 were deduced to cause amino acid exchanges, were detected, Suggesting that the chicken Mx gene is very polymorphic. Transfected cell clones expressing chicken Mx mRNA were established after the Mx cDNA was constructed with an expression vector and introduced into mouse 3T3 cells, and the Mx genes from some breeds were demonstrated to confer positive antiviral responses to influenza virus and vesicular stomatitis virus. On the basis of the comparison among the antiviral activities associated with many Mx variations, a specific amino acid substitution at position 631 (Ser to Asn) was considered to determine the antivirally positive or negative Mx gene. Thus, a single amino acid Substitution influences the antiviral activity of Mx in domesticated chickens.

DOI： 10.1101/gr.210702

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-BLACKWELL  

The combination of the Kit(W) or Kit(W-n) mutant alleles and Kits from Mus spretus results in male hybrid sterility with small testes. In the present study, reproduction of the combination between Kit(W-v) and Kit(s) alleles was examined. The Kit(W-v)/Kit(s) male was fertile and the histologic structure was normal; the seminiferous tubules showed all of the normal stages of spermatogenesis. The postnatal development of the testis at 8, 12, 16 and 20 days was also studied in the fertile +(Kit)/+(Kit) and Kit(W-v)/Kit(s) males and the sterile Kit(W)/Kit(s). The results showed that at 8 days there was no noticeable difference among the three genotype combinations, while from 12 to 20 days spermatogenesis in the Kit(w)/Kit(s) male nearly stopped before the meiosis stage. The expression of Kit receptor protein from the Kit(s) allele in the sterile testis of the Kit(w)/Kit(s) male was confirmed using western blot analysis. The Kit ligand derived from M. spretus showed two amino acid changes in the extracellular domain compared with that from C57BL and it appears that the ligand-receptor interaction between C57BL and SPR may influence the male hybrid sterility of Kit(w)/Kit(s).

DOI： 10.1046/j.1440-169X.2001.00598.x

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-LISS  

During mammalian spermatogenesis, many specific molecules show the dynamics of expression and elimination, corresponding with the morphological differentiation of germ cells. We have isolated a novel cDNA designated F77 from mouse testis by cDNA subtractive hybridization between normal and sterile mice, using the C57BL/6 congenic strain for the hybrid sterility-3 (Hst-3) allele from Mus spretus. The full-length F77 mRNA was 3.4 kb and showed significant nonmatching with entries in the databases. F77 was mapped at a proximal position between D8Mit212 and D8Mit138 on mouse chromosome 8, in which no corresponding genes related to its nucleotide sequence were found. F77 mRNA was not detected in any other organs except the testis of adult fertile mice. F77 protein was only seen in normal adult testis and epididymis. in contrast to normal C57BL/6 mice, F77 mRNA and protein were not seen in germ cell-deficient Kit(W)/Kit(Wv) mice. By in situ hybridization, F77 mRNA was detected mainly at round spermatids in the sexually mature testis, and immunohistochemical analysis revealed that F77 protein was located at the tail of elongated spermatids. We are proposing the name, sperm-tail-associated protein (Stap), for the gene encoding F77 cDNA. (C) 2001 Wiley-Liss, Inc.

DOI： 10.1002/mrd.1041

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：KLUWER ACADEMIC/PLENUM PUBL  

Much is known about the antiviral activity of Mx proteins in species such as mouse and human. In the mouse, loss of resistibility to influenza virus has been shown to be due to specific polymorphisms in the Mx gene. This gene is therefore an interesting candidate gene for disease resistance in farm animals. The porcine Mx1 gene has already been identified and characterized based on its homology with mouse Mx1; however until now no evidence of polymorphisms in the porcine gene has been reported. In this study, we have found two new polymorphisms in exon 14 of porcine Mx I by DNA sequencing and confirmed their presence in different breeds, using polymerase chain reaction (PCR)-restriction fragment length polymorphisms (RFLP) with NarI and NaeI restriction enzymes. On the basis of the deduced amino acid sequence, one allele contains a deletion that may result in a frameshift to yield several amino acid substitutions and extension of the carboxyl terminal region of Mx1 protein. The deletion allele, Mx1(c), was found to be segregating in Landrace, Berkshire, Duroc, Hampshire, and Yucatan miniature pig. A second point mutation, Mx1(b), was detected in Meishan and two Vietnamese native pig breeds. All other breeds tested were fixed for the Mx1(a) allele that is identical to the sequence reported previously. It will be interesting to determine if the Mx1(c) deletion is associated with variation in resistance to the myxovirus family in the pig.

DOI： 10.1023/A:1010230715605

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：KLUWER ACADEMIC/PLENUM PUBL  

Two congenic strains, C57BL-Kit(w) and C57BL-Kit(s), were generated. The Kit(w) allele originated from strain WB-Kit(w) and the Kit(s) allele from Mus spretus. The Kit(w)/Kit(s) males showed hybrid sterility with small testes, but the females were fertile. The development of the seminiferous tubules of Kit(W)/Kit(s) males stopped before the spermatocyte stage and they were almost free of sperm. The Kit gene is located at position 42 on chromosome 5. We investigated in the C57BL-Kits congenic strain which part of the chromosomal region adjacent to the Kits allele is introduced from SPR into a C57BL background. The region between positions 42 and 44 was derived from SPR. Eleven amino acid substitutions of the Kit(s) cDNA were detected by comparison with the sequence data of the +(Kit) cDNA from C57BL; seven were in the extracellular domain, one in the transmembrane domain, two in the kinase I domain, and one in the carboxy-terminal tail. The Kit mRNA derived from both Kit(w) and Kit(s) alleles was expressed in the sterile testes of Kit(w)/Kit(s) males.

DOI： 10.1023/A:1010217924328

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：JAPAN SOC VET SCI  

Chronic stimulation of the beta3-adrenergic receptor (AR) in obese animals resulted in a reduced adiposity associated with an increased expression of thermogenic uncoupling protein (UCP)1 in adipose tissues. In this study, the mRNA expression of newly cloned UCP isoforms (UCP2 and UCP3) were examined in obese yellow KK and C57BL control mice. UCP2 mRNA was found in all tissues examined, with higher levels in adipose tissues and skeletal muscle of the obese mice. UCP3 mRNA was expressed in skeletal muscle, heart and brown adipose tissue similarly in the two mouse strains. Daily injection of a selective beta3-adrenergic agonist, CL316,243 (0.1 mg/kg), for 10 days resulted in a marked reduction of white fat pad weight and 1.8 similar to4.8-fold increase in the mRNA levels of UCP2 and UCP3 in skeletal muscle of obese mice. No noticeable change in the UCP2 and 3 mRNA levels was found in brown and white adipose tissues. It was also found that CL316,243 injection produced a marked and sustained elevation of the plasma free fatty acid level. These results, together with our previous Endings of the fatty acid-induced UCP expression in a myocyte cell line in vitro, suggest that the beta3-AR agonist-induced UCP expression in skeletal muscle may be mediated through the elevated plasma free fatty acids. It was also suggested that anti-obesity effect of beta3-AR agonists is attributable to increased thermogenesis not only by UCP1 but also by UCP2 and UCP3.

DOI： 10.1292/jvms.63.309

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER-VERLAG WIEN  

The antiviral potential of Mx2 protein remains unknown, because the Mx2 gene in commonly used strains of laboratory mice is nonfunctional. Our previous study showed that functional Mx2 protein in some feral-origin strains was induced upon interferon treatment, was localized in the cytoplasm, and inhibited vesicular stomatitis virus replication. In the present study, we have demonstrated chat the embryonic fibroblastic cells from a feral-origin strain (SPR) expressed 74 kDa Mx2 protein, which prevented the accumulation of viral transcripts and proteins of hantaviruses when the Mx2 gene was constitutively expressed in transfected Vero cells. Furthermore, the cells showed significantly lower titers of the virus than control cells. In contrast, influenza virus replication was not affected by the expression of Mx2 protein in the Vero cells.

DOI： 10.1007/s007050170189

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：GEORG THIEME VERLAG  

Wild-type or mutated human beta 3-adrenergic receptor (Trp64Arg) cDNAs were stably expressed in mouse 3T3-L1 cells. Saturation binding study using a beta-adrenergic ligand revealed that there was no significant difference in the receptor density and the equilibrium dissociation constant between the two cell lines. However, the ability of the mutant beta 3-adrenergic receptor to accumulate cyclic AMP (cAMP) in response to isoproterenol was much reduced and Kact for cAMP accumulation was lowered as compared to the wild type receptor. The amount of alpha subunit of stimulatory GTP-binding protein (GS alpha) and adenylyl cyclase activity in response to forskolin were not different in the two cell lines. The responses of the mutant receptor to epinephrine, norepinephrine and L-755,507, a highly specific agonist for human beta 3-adrenergic receptor, were also reduced, but the reduction of Kact for L-755,507 was more evident than other agonists tested. The cAMP accumulation in response to some conventional beta 3 agonists was less than 10% of that to isoproterenol even in the cells expressing the wild type receptor. These results suggest that the Trp64Arg mutant beta 3-adrenergic receptor has less ability to stimulate adenylyl cyclase, and that lipolytic activity through the beta 3-adrenergic receptor by catecholamines in subjects carrying this mutation might be suppressed.

DOI： 10.1055/s-2007-978597

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：JAPAN SOC VET SCI  

Brown adipose tissue (BAT) is the major site of non-shivering thermogenesis in rodents. Rapid angiogenesis is induced in association with adaptive hyperplasia of this tissue when the animal is exposed to cold. We demonstrated previously adrenergic activation of mRNA expression of vascular endothelial growth factor (VEGF) in rat BAT and its possible contribution to the cold-induced angiogenesis in this tissue. In the present study, we examined the effect of cold exposure on mRNA expression of other two angiogenic factors. VEGF-B and basic fibroblast growth factor (bFGF), in rats. Conventional Northern blot analysis revealed abundant mRNA expression of VEGF-B as well as VEGF, but not bFGF, in BAT. When rats were exposed to cold at 4 degrees C, the VEGF mRNA level was increased by 2.7-fold in 1-4 hr and returned to the basal level within 24 hr. In contrast, the VEGF-B mRNA level did not change throughout the course of cold exposure. A significant expression of bFGF mRNA was detected in BAT by reverse transcription polymerase chain reaction (RT-PCR). To evaluate the tissue bFGF mRNA level quantitatively, a competitive RT-PCR method was developed using a shorter RNA fragment as a competitor. The bFGF mRNA level in BAT was found to increase by 2.3-fold in 4 hr and decreased to the basal level within 24 hr after cold exposure. These results suggest that cold exposure leads to induce VEGF and bFGF rapidly and transiently in BAT, which in turn stimulate the proliferation of vascular endothelial cells in this tissue.

DOI： 10.1292/jvms.61.403

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記述言語：日本語   掲載種別：学位論文（博士）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：JAPAN ENDOCRINE SOCIETY  

The inhibitory effect of beta(3)-adrenoceptor agonists on the ob gene in brown adipose tissue (BAT) and white adipose tissue (WAT) is now well documented both in viva in lean animals and in vitro, but the reported effects of beta(3)-adrenoceptor agonists on ob gene expression in obese animals remain controversial. We investigated whether ob gene expression in BAT and WAT is reduced by acute and chronic administrations of a beta(3)-adrenoceptor agonist, CL316,243 (CL). The ob gene mRNA levels in BAT, perimetric and inguinal WAT of obese Yellow KK mice were about 4-fold higher than those of lean controls. Acute exposure (6 h) to CL decreased ob gene mRNA levels in three fat depots in both animals. Chronic exposure (10 days) to CL also decreased ob gene mRNA levels in BAT, perimetric, and inguinal WAT in both animals. We concluded that acute and chronic regulation by a beta(3)-adrenoceptor agonist suppressed ob gene expression in obese Yellow KK mice and lean cotrols.

DOI： 10.1507/endocrj.45.647

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PORTLAND PRESS  

Cold exposure produces adaptive hyperplasia and growth of brown adipose tissue (BAT), the major site of non-shivering thermogenesis in rodents, associated with increased angiogenesis in this tissue. Vascular endothelial growth factor (VEGF), one of the most potent angiogenic factors, was found to be expressed abundantly in BAT of the rat. When rats were exposed to cold at 4 degrees C, the VEGF mRNA level in BAT was increased by 2-3-fold in 1-4 h, but returned to the basal level within 24 h. VEGF expression in other tissues such as heart, kidney and lung did not change after cold exposure. The cold-induced increase in VEGF mRNA was abolished by surgical sympathetic denervation, but mimicked by administration of noradrenaline or a beta(3)-adrenoceptor agonist CL316,243, indicating the critical role of the beta-adrenergic pathway in VEGF expression in BAT. Among three isoforms of VEGF, the mRNA of a short form (VEGF120) lacking heparin-binding activity was preferentially increased after cold exposure and treatment with the adrenergic agonists. These results suggest that cold exposure activates the sympathetic nerves and leads to a rapid increase in synthesis of VEGF in BAT, which in turn stimulates the proliferation of surrounding vascular endothelial cells.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：LIPPINCOTT-RAVEN PUBL  

Possible roles of prostaglandins (PGs) in interleukin-l (Il-l)-induced activation of noradrenergic neurons were examined by assessing norepinephrine (NE) turnover in the brain and peripheral organs of rats, An intraperitoneal injection of human recombinant IL-1 beta accelerated NE turnover in the hypothalamus, spleen, lung, diaphragm, and pancreas. A similar increase in NE turnover was also observed after intracerebroventricular injection of corticotropin-releasing hormone (CRH), Pretreatment with indomethacin (cyclooxygenase inhibitor) abolished the IL-1-induced, but not the CRH-induced, increase in hypothalamic and splenic NE turnover. To elucidate which eicosanoid-cyclooxygenase product(s) is responsible for accelerating NE turnover, PGD(2), PGE(2), PGF(2 alpha), U-46619 (stable thromboxane A(2) analogue), or carbacyclin (stable prostacyclin analogue) was administered intracerebroventricularly. Among them, PGE, was the only eicosanoid effective in increasing NE turnover in spleen, whereas PGD(2) was effective in the hypothalamus. The stimulative effect of PGD(2) was abolished by pretreatment with intracerebroventricular injection of a CRH antiserum. These results suggest that the action of IL-1 is mediated through PGD, production to activate the noradrenergic neurons in the hypothalamus, and through PGE(2) production to increase sympathetic nerve activity in spleen.

DOI： 10.1046/j.1471-4159.1995.65062742.x

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