Updated on 2025/05/17

写真a

 
Kuramoto Eriko
 
Organization
Research Field in Dentistry, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course Neurology Assistant Professor
Title
Assistant Professor
Homepage
http://tgoto9.wixsite.com/gotolab-site
Profile
視床-大脳皮質の相互結合が高次脳機能、たとえば運動学習、認知などにおいて、果たしている機能を明らかにしたい。
External link

Degree

  • 医学博士 ( 2019.3   京都大学 )

Research Interests

  • cortex

  • thalamus

  • neuroanatomy

  • Higher order thalamic nuclei

  • Thalamocortical projection

  • Thalamus

  • 神経解剖

  • Pain

  • Frontal cortex

Research Areas

  • Life Science / Oral biological science

  • Life Science / Anatomy and histopathology of nervous system

Research History

  • Kagoshima University   Research Field in Dentistry, Medical and Dental Sciences Area Graduate School of Medical and Dental Sciences Advanced Therapeutics Course   Assistant Professor

    2014.9

  • Kagoshima University   Graduate School of Medical and Dental Sciences   Assistant Professor

    2014.9

  • 京都大学 医学(系)研究科(研究院)   研究員

    2011

Professional Memberships

  • 歯科基礎医学会

    2015.6

  • Society for Neuroscience

    2010.1

  • 解剖学会

    2005.4

  • 神経科学学会

    2004.4

  • JAPANESE ASSOCIATION FOR ORAL BIOLOGY

  • THE JAPANESE ASSOCIATION OF ANATOMISTS

  • THE JAPAN NEUROSCIENCE SOCIETY

  • Society for Neuroscience

▼display all

 

Papers

  • Tetsuya Goto, Eriko Kuramoto, Haruki Iwai, Atsushi Yamanaka .  Cytoarchitecture and intercellular interactions in the trigeminal ganglion: Associations with neuropathic pain in the orofacial region. .  Journal of oral biosciences66 ( 3 ) 485 - 490   2024.7International journal

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    Language：English   Publishing type：Research paper (scientific journal)  

    BACKGROUND: Disorders of the trigeminal nerve, a sensory nerve of the orofacial region, often lead to complications in dental practice, including neuropathic pain, allodynia, and ectopic pain. Management of these complications requires an understanding of the cytoarchitecture of the trigeminal ganglion, where the cell bodies of the trigeminal nerve are located, and the mechanisms of cell-cell interactions. HIGHLIGHTS: In the trigeminal ganglion, the ganglion, satellite, Schwann, and immune cells coexist and interact. Cell-cell interactions are complex and occur through direct contact via gap junctions or through mediators such as adenosine triphosphate, nitric oxide, peptides, and cytokines. Interactions between the nervous and immune systems within the trigeminal ganglion may have neuroprotective effects during nerve injury or may exacerbate inflammation and produce chronic pain. Under pathological conditions of the trigeminal nerve, cell-cell interactions can cause allodynia and ectopic pain. Although cell-cell interactions that occur via mediators can act at some distance, they are more effective when the cells are close together. Therefore, information on the three-dimensional topography of the trigeminal ganglion cells is essential for understanding the pathophysiology of ectopic pain. CONCLUSIONS: A three-dimensional map of the somatotopic localization of trigeminal ganglion neurons revealed that ganglion cells innervating distant orofacial regions are often apposed to each other, interacting with and potentially contributing to ectopic pain. Elucidation of the complex network of mediators and their receptors responsible for intercellular communication within the trigeminal ganglion is essential for understanding ectopic pain.

    DOI： 10.1016/j.job.2024.07.003

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  • Eriko Kuramoto, Makoto Fukushima, Ryozo Sendo, Sachi Ohno, Haruki Iwai, Atsushi Yamanaka, Mitsutaka Sugimura, Tetsuya Goto .  Three-dimensional topography of rat trigeminal ganglion neurons using a combination of retrograde labeling and tissue-clearing techniques .  Journal of Comparative Neurology532 ( 2 ) e25584   2024.2

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    The trigeminal nerve is the sensory afferent of the orofacial regions and divided into three major branches. Cell bodies of the trigeminal nerve lie in the trigeminal ganglion and are surrounded by satellite cells. There is a close interaction between ganglion cells via satellite cells, but the function is not fully understood. In the present study, we clarified the ganglion cells’ three-dimensional (3D) localization, which is essential to understand the functions of cell–cell interactions in the trigeminal ganglion. Fast blue was injected into 12 sites of the rat orofacial regions, and ganglion cells were retrogradely labeled. The labeled trigeminal ganglia were cleared by modified 3DISCO, imaged with confocal laser-scanning microscopy, and reconstructed in 3D. Histograms of the major axes of the fast blue-positive somata revealed that the peak major axes of the cells innervating the skin/mucosa were smaller than those of cells innervating the deep structures. Ganglion cells innervating the ophthalmic, maxillary, and mandibular divisions were distributed in the anterodorsal, central, and posterolateral portions of the trigeminal ganglion, respectively, with considerable overlap in the border region. The intermingling in the distribution of ganglion cells within each division was also high, in particular, within the mandibular division. Specifically, intermingling was observed in combinations of tongue and masseter/temporal muscles, maxillary/mandibular molars and masseter/temporal muscles, and tongue and mandibular molars. Double retrograde labeling confirmed that some ganglion cells innervating these combinations were closely apposed. Our data provide essential information for understanding the function of ganglion cell–cell interactions via satellite cells.

    DOI： 10.1002/cne.25584

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  • Sonoda R., Kuramoto E., Minami S., Matsumoto S.E., Ohyagi Y., Saito T., Saido T., Noguchi K., Goto T. .  Reduced Autophagy in Aged Trigeminal Neurons Causes Amyloid β Diffusion .  Journal of Dental Research102 ( 8 ) 938 - 946   2023.7

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    Language：Japanese   Publisher：Journal of Dental Research  

    The relationship between oral health and the development of Alzheimer’s disease (AD) in the elderly is not yet well understood. In this regard, the association between aging or neurodegeneration of the trigeminal nervous system and the accumulation of amyloid-β(1–42) (Aβ42) oligomers in the pathogenesis of AD is unknown. We focused on selective autophagy in the trigeminal mesencephalic nucleus (Vmes) and the diffusion of Aβ42 oligomers with respect to aging of the trigeminal nervous system and whether the degeneration of Vmes neurons affects the diffusion of Aβ42 oligomers. We used female 2- to 8-mo-old transgenic 3xTg-AD mice and AppNL-G-F knock-in mice and immunohistochemically examined aging-related changes in selective autophagy and Aβ42 oligomer processing in the Vmes, which exhibits high amyloid-β (Aβ) expression. We induced degeneration of Vmes neurons by extracting the maxillary molars and examined the changes in Aβ42 oligomer kinetics. Autophagosome-like membranes, which stained positive for Aβ, HO-1, and LC3B, were observed in Vmes neurons of 3xTg-AD mice, while there was weak immunoreactivity of the membranes for intraneuronal Aβ in AppNL-G-F mice. By contrast, there was strong immunopositivity for extracellular Aβ42 oligomers with the formation of Aβ42 oligomer clusters in AppNL-G-F mice. The expression of Rubicon, which indicates age-related deterioration of autophagy, increased the diffusion of Aβ42 oligomer with the age of Vmes neurons. Tooth extraction increased the extracellular immunopositivity for Aβ42 oligomers in AppNL-G-F mice. These results suggest that autophagy maintains homeostasis in Vmes neurons and that deterioration of autophagy due to aging or neurodegeneration leads to the diffusion of Aβ42 oligomers into the extracellular space and possibly the development of AD.

    DOI： 10.1177/00220345231156095

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  • Yukie Shirakawa, Sachi N. Ohno, Kanae A. Yamagata, Eriko Kuramoto, Yoshiaki Oda, Takahiro J. Nakamura, Wataru Nakamura, Mitsutaka Sugimura .  Circadian rhythm of PERIOD2::LUCIFERASE expression in the trigeminal ganglion of mice .  Frontiers in Neuroscience17   1142785   2023.3

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    Introduction

    The trigeminal nerve conveys delicate sensations such as warmth, pain, and tactile pressure in the oral and facial regions, and most trigeminal afferent cell bodies are located in the trigeminal ganglion. Our previous study has shown that sensations in trigeminal nerve innervated areas, specifically in the maxillofacial region, exhibit diurnal variation and that sensitivity changes time-dependently. In this study, we aimed to clarify the rhythm of expression of clock gene in the trigeminal ganglion of mice to elucidate the mechanism of circadian regulation in the same area.

    Methods

    Immunohistochemistry examined the expression of the PER2 protein in the suprachiasmatic nucleus and trigeminal ganglion of wild-type mice. To measure gene expression as bioluminescence, PERIOD2::LUCIFERASE knock-in (PER2::LUC) mice were used. Unilateral trigeminal ganglion and brain sections including the suprachiasmatic nucleus were incubated ex vivo. Bioluminescence levels were then measured using a highly sensitive photodetector. The same experiments were then conducted with Cry1 gene-deficient (Cry1<sup>−/−</sup>) or Cry2 gene-deficient (Cry2<sup>−/−</sup>) mice.

    Results

    In the trigeminal ganglion, immunohistochemistry localized PER2 protein expression within the neuronal cell body. Mouse trigeminal ganglion ex vivo tissues showed distinct circadian oscillations in PER2::LUC levels in all genotypes, wild-type, Cry1<sup>−/−</sup>, and Cry2<sup>−/−</sup>. The period was shorter in the trigeminal ganglion than in the suprachiasmatic nucleus; it was shorter in Cry1<sup>−/−</sup> and longer in Cry2<sup>−/−</sup> mice than in the wild-type mice.

    Conclusion

    The expression of Per2 in neurons of the trigeminal ganglion in ex vivo culture and the oscillation in a distinct circadian rhythm suggests that the trigeminal ganglion is responsible for the relay of sensory inputs and temporal gating through autonomous circadian oscillations.

    DOI： 10.3389/fnins.2023.1142785

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  • Eriko Kuramoto, Ayano Kitawaki, Takakazu Yagi, Hiroshi Kono, Shin-Ei Matsumoto, Hiromitsu Hara, Yasumasa Ohyagi, Haruki Iwai, Atsushi Yamanaka, Tetsuya Goto .  Development of a system to analyze oral frailty associated with Alzheimer's disease using a mouse model .  Frontiers in Aging Neuroscience14   935033   2022.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    The rapid aging of the population makes the detection and prevention of frailty increasingly important. Oral frailty has been proposed as a novel frailty phenotype and is defined as a decrease in oral function coexisting with a decline in cognitive and physical functions. Oral frailty has received particular attention in relation to Alzheimer's disease (AD). However, the pathomechanisms of oral frailty related to AD remain unknown. It is assumed that the mesencephalic trigeminal nucleus (Vmes), which controls mastication, is affected by AD pathology, and as a result, masticatory function may be impaired. To investigate this possibility, we included male 3 × Tg-AD mice and their non-transgenic counterpart (NonTg) of 3–4 months of age in the present study. Immunohistochemistry revealed amyloid-β deposition and excessive tau phosphorylation in the Vmes of 3 × Tg-AD mice. Furthermore, vesicular glutamate transporter 1-immunopositive axon varicosities, which are derived from Vmes neurons, were significantly reduced in the trigeminal motor nucleus of 3 × Tg-AD mice. To investigate whether the AD pathology observed in the Vmes affects masticatory function, we analyzed electromyography of the masseter muscle during feeding. The 3 × Tg-AD mice showed a significant delay in masticatory rhythm compared to NonTg mice. Furthermore, we developed a system to simultaneously record bite force and electromyography of masseter, and devised a new method to estimate bite force during food chewing in mice. Since the muscle activity of the masseter showed a high correlation with bite force, it could be accurately estimated from the muscle activity. The estimated bite force of 3 × Tg-AD mice eating sunflower seeds was predominantly smaller than that of NonTg mice. However, there was no difference in masseter weight or muscle fiber cross-sectional area between the two groups, suggesting that the decreased bite force and delayed mastication rhythm observed in 3 × Tg-AD mice were not due to abnormality of the masseter. In conclusion, the decreased masticatory function observed in 3 × Tg-AD mice was most likely caused by AD pathology in the Vmes. Thus, novel quantitative analyses of masticatory function using the mouse model of AD enabled a comprehensive understanding of oral frailty pathogenesis.

    DOI： 10.3389/fnagi.2022.935033

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  • Kuramoto E., Tanaka Y.R., Hioki H., Goto T., Kaneko T. .  Local Connections of Pyramidal Neurons to Parvalbumin-Producing Interneurons in Motor-Associated Cortical Areas of Mice .  eNeuro9 ( 1 )   2022.1

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    Language：Japanese   Publisher：eNeuro  

    Parvalbumin (PV)-producing neurons are the largest subpopulation of cortical GABAergic interneurons, which mediate lateral, feedforward, and feedback inhibition in local circuits and modulate the activity of pyramidal neurons. Clarifying the specific connectivity between pyramidal and PV neurons is essential for understanding the role of PV neurons in local circuits. In the present study, we visualized somas and dendrites of PV neurons using transgenic mice in which PV neurons specifically express membrane-targeted GFP, and intracellularly labeled local axons of 26 pyramidal neurons in layers 2–6 in acute slices of the motor-associated cortex from transgenic mice. We mapped morphologically distribution of inputs from a pyramidal neuron to PV neurons based on contact sites (appositions) between the axons from an intracellularly filled pyramidal neuron and the dendrites of PV neurons. Layer 6 corticothalamic (CT)-like pyramidal neurons formed appositions to PV neurons at a significantly higher rate than other pyramidal neurons. The percentage of apposed varicosities to all the labeled varicosities of layer 6 CT-like neurons was 28%, and that of the other pyramidal neurons was 12– 19%. Layer 6 CT-like neurons preferentially formed appositions with PV neurons in layers 5b–6, while other pyramidal neurons uniformly formed appositions with PV neurons in all layers. Furthermore, both layer 6 CT-like and corticocortical-like neurons more frequently formed compound appositions, where two or more appo-sitions were located on a dendritic branch, than other pyramidal neurons. Layer 6 CT neurons may contribute to intracortical information processing through preferential connections with PV neurons in layers 5b–6.

    DOI： 10.1523/ENEURO.0567-20.2021

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  • 倉本 恵梨子 .  遺伝子工学を用いた神経結合の可視化 .  臨床神経科学6   2022遺伝子工学を用いた神経結合の可視化

  • Haruki Iwai, Koji Ataka, Hajime Suzuki, Ashis Dhar, Eriko Kuramoto, Atsushi Yamanaka, Tetsuya Goto .  Tissue-resident M2 macrophages directly contact primary sensory neurons in the sensory ganglia after nerve injury. .  Journal of neuroinflammation18 ( 1 ) 227 - 227   2021.12International journal

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    BACKGROUND: Macrophages in the peripheral nervous system are key players in the repair of nerve tissue and the development of neuropathic pain due to peripheral nerve injury. However, there is a lack of information on the origin and morphological features of macrophages in sensory ganglia after peripheral nerve injury, unlike those in the brain and spinal cord. We analyzed the origin and morphological features of sensory ganglionic macrophages after nerve ligation or transection using wild-type mice and mice with bone-marrow cell transplants. METHODS: After protecting the head of C57BL/6J mice with lead caps, they were irradiated and transplanted with bone-marrow-derived cells from GFP transgenic mice. The infraorbital nerve of a branch of the trigeminal nerve of wild-type mice was ligated or the infraorbital nerve of GFP-positive bone-marrow-cell-transplanted mice was transected. After immunostaining the trigeminal ganglion, the structures of the ganglionic macrophages, neurons, and satellite glial cells were analyzed using two-dimensional or three-dimensional images. RESULTS: The number of damaged neurons in the trigeminal ganglion increased from day 1 after infraorbital nerve ligation. Ganglionic macrophages proliferated from days 3 to 5. Furthermore, the numbers of macrophages increased from days 3 to 15. Bone-marrow-derived macrophages increased on day 7 after the infraorbital nerve was transected in the trigeminal ganglion of GFP-positive bone-marrow-cell-transplanted mice but most of the ganglionic macrophages were composed of tissue-resident cells. On day 7 after infraorbital nerve ligation, ganglionic macrophages increased in volume, extended their processes between the neurons and satellite glial cells, and contacted these neurons. Most of the ganglionic macrophages showed an M2 phenotype when contact was observed, and little neuronal cell death occurred. CONCLUSION: Most of the macrophages that appear after a nerve injury are tissue-resident, and these make direct contact with damaged neurons that act in a tissue-protective manner in the M2 phenotype. These results imply that tissue-resident macrophages signal to neurons directly through physical contact.

    DOI： 10.1186/s12974-021-02283-z

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  • Eriko Kuramoto, Yasuhiro Tanaka, Hiroyuki Hioki, Tetsuya Goto, Takeshi Kaneko .  Local Connections of Pyramidal Neurons to Parvalbumin-Producing Interneurons in Motor-Associated Cortical Areas of Mice .  eNeuro   2021.12Local Connections of Pyramidal Neurons to Parvalbumin-Producing Interneurons in Motor-Associated Cortical Areas of MiceReviewed

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

  • Dhar Ashis, Kuramoto Eriko, Fukushima Makoto, Iwai Haruki, Yamanaka Atsushi, Goto Tetsuya .  The Periodontium Damage Induces Neuronal Cell Death in the Trigeminal Mesencephalic Nucleus and Neurodegeneration in the Trigeminal Motor Nucleus in C57BL/6J Mice .  ACTA HISTOCHEMICA ET CYTOCHEMICA54 ( 1 ) 11 - 19   2021.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY  

    <p>Proprioception from masticatory apparatus and periodontal ligaments comes through the trigeminal mesencephalic nucleus (Vmes). We evaluated the effects of tooth loss on neurodegeneration of the Vmes and trigeminal motor nucleus (Vmo). Bilateral maxillary molars of 2-month-old C57BL/6J mice were extracted under anesthesia. Neural projections of the Vmes to the periodontium were confirmed by injecting Fluoro-Gold (FG) retrogradely into the extraction sockets, and for the anterograde labeling adeno-associated virus encoding green fluorescent protein (AAV-GFP) was applied. For immunohistochemistry, Piezo2, ATF3, Caspase 3, ChAT and TDP-43 antibodies were used. At 1 month after tooth extraction, the number of Piezo2-immunoreactive (IR) Vmes neurons were decreased significantly. ATF3-IR neurons were detected on day 5 after tooth extraction. Dead cleaved caspase-3-IR neurons were found among Vmes neurons on days 7 and 12. In the Vmo, neuronal cytoplasmic inclusions (NCIs) formation type of TDP-43 increased at 1 and 2 months after extraction. These indicate the existence of neural projections from the Vmes to the periodontium in mice and that tooth loss induces the death of Vmes neurons followed by TDP-43 pathology in the Vmo. Therefore, tooth loss induces Vmes neuronal cell death, causing Vmo neuro­degeneration and presumably affecting masticatory function.</p>

    DOI： 10.1267/ahc.20-00036

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  • Dhar Ashis, Kuramoto Eriko, Fukushima Makoto, Iwai Haruki, Yamanaka Atsushi, Goto Tetsuya .  C57BL/6マウスにおいて歯周組織損傷は三叉神経中脳路核の神経細胞死と三叉神経運動核の神経変性を誘導する(The Periodontium Damage Induces Neuronal Cell Death in the Trigeminal Mesencephalic Nucleus and Neurodegeneration in the Trigeminal Motor Nucleus in C57BL/6J Mice) .  Acta Histochemica et Cytochemica54 ( 1 ) 11 - 19   2021.2C57BL/6マウスにおいて歯周組織損傷は三叉神経中脳路核の神経細胞死と三叉神経運動核の神経変性を誘導する(The Periodontium Damage Induces Neuronal Cell Death in the Trigeminal Mesencephalic Nucleus and Neurodegeneration in the Trigeminal Motor Nucleus in C57BL/6J Mice)

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    Language：English   Publisher：日本組織細胞化学会  

    咀嚼器官と歯根膜からの自己受容性感覚は三叉神経中脳路核(Vmes)を介する。Vmesと三叉神経運動核(Vmo)の変性に対する歯の欠損の影響を調べた。マウスの両側の上顎大臼歯を抜歯した。Vmesの歯周組織へのニューロン投射は、フルオロ-ゴールド(FG)を抜歯創に逆行性に注射して確認し、順行性標識には緑色蛍光蛋白質コードアデノ関連ウイルスを使用した。免疫組織化学検査ではPiezo2、ATF3、カスパーゼ3、ChATとTDP-43抗体を使用した。抜歯1ヵ月後に、Piezo2の免疫反応性(IR)Vmesニューロンの数は有意に低下した。ATF3-IRニューロンは抜歯の5日後に、死んだ開裂カスパーゼ3-IRニューロンは7、12日後に検出された。VmoではニューロンのTDP-43細胞質内封入体が、抜歯1、2ヵ月後に増加した。マウスでは歯の欠損はVmesニューロン細胞死とVmo神経変性を誘発し、咀嚼機能に影響すると考えられた。

  • Ayako Niiro, Sachi N Ohno, Kanae A Yamagata, Kazuaki Yamagata, Kazuo Tomita, Eriko Kuramoto, Yoshiaki Oda, Takahiro J Nakamura, Wataru Nakamura, Mitsutaka Sugimura .  Diurnal Variation in Trigeminal Pain Sensitivity in Mice. .  Frontiers in neuroscience15   703440 - 703440   2021International journal

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    Management of time and circadian disruption is an extremely important factor in basic research on pain and analgesia. Although pain is known to vary throughout the day, the mechanism underlying this circadian variation remains largely unknown. In this study, we hypothesized that the process of pain transmission to the central nervous system (after receiving nociceptive stimuli from outside the body) would show day-night differences. Ten-week-old male mice were kept under a strict 12/12-h light/dark cycle for at least 10 days. Formalin was then injected into the second branch region of the trigeminal nerve and the duration of pain-related behaviors (PRBs) was assessed. Immunohistochemical staining was then performed, and the c-Fos-immunopositive cells in the trigeminal spinal tract subnucleus caudalis (Sp5C) were counted. The results showed that the duration of PRBs was longer and the number of c-Fos immunopositive cells in the Sp5C was higher at nighttime than during the day. In addition, the trigeminal ganglia (TG) were extracted from the mice and examined by quantitative real-time PCR to evaluate the daytime and nighttime expression of nociceptive receptors. The results showed that the mRNA expression of transient receptor potential ankyrin 1 in the TG was significantly higher at night than during the day. These results suggest that pain in the trigeminal nerve region is more intense at nighttime, when rodents are active, than during the daytime, partly due to differences in nociceptor expression.

    DOI： 10.3389/fnins.2021.703440

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  • Kayo Nishimura, Masahiro Ohta, Mitsuru Saito, Yukako Morita-Isogai, Hajime Sato, Eriko Kuramoto, Dong Xu Yin, Yoshinobu Maeda, Takeshi Kaneko, Takashi Yamashiro, Kenji Takada, Seog Bae Oh, Hiroki Toyoda, Youngnam Kang .  Electrophysiological and morphological properties of α and γ motoneurons in the rat trigeminal motor nucleus .  Frontiers in Cellular Neuroscience12   9   2018.1Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media S.A.  

    The muscle contraction during voluntary movement is regulated by activities of α- and γ-motoneurons (αMNs and γMNs, respectively). The tension of jaw-closing muscles can be finely tuned over a wide range. This excellent function is likely to be achieved by the specific populations of αMNs innervating jaw-closing muscles. Indeed, we have recently demonstrated that in the rat dorsolateral trigeminal motor nucleus (dl-TMN), the size distribution of αMNs was bimodal and the population of smaller αMNs showed a size distribution similar to that of γMNs, by immunohistochemically identifying αMNs and γMNs based on the expressions of estrogen-related receptor gamma (Err3) and neuronal DNA binding protein NeuN together with ChAT. This finding suggests the presence of αMNs as small as γMNs. However, differences in the electrophysiological membrane properties between αMNs and γMNs remain unknown also in the dl-TMN. Therefore, in the present study, we studied the electrophysiological membrane properties of MNs in the dl-TMN of infant rats at postnatal days 7–12 together with their morphological properties using whole-cell current-clamp recordings followed by immunohistochemical staining with an anti-NeuN and anti-ChAT antibodies. We found that the ChAT-positive and NeuN-positive αMNs were divided into two subclasses: the first one had a larger cell body and displayed a 4-aminopyridine (4-AP)-sensitive current while the second one had a smaller cell body and displayed a less prominent 4-AP-sensitive current and a low-threshold spike, suitable for their orderly recruitment. We finally found that γMNs showing ChAT-positive and NeuN-negative immunoreactivities had smaller cell bodies and displayed an afterdepolarization mediated by flufenamate-sensitive cation current. It is suggested that these electrophysiological and morphological features of MNs in the dl-TMN are well correlated with the precise control of occlusion.

    DOI： 10.3389/fncel.2018.00009

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  • Unzai T, Kuramoto E, Kaneko T, Fujiyama F. .  Quantitative analysis of the projection of individual neurons from the midline thalamic nuclei to the striosome and matrix compartments of the rat striatum. Cerebral Cortex .  Cerebral Cortex   2016Quantitative analysis of the projection of individual neurons from the midline thalamic nuclei to the striosome and matrix compartments of the rat striatum. Cerebral CortexReviewed

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  • Eriko Kuramoto, Sachi Ohno, Takahiro Furuta, Tomo Unzai, Yasuhiro R. Tanaka, Hiroyuki Hioki, *Takeshi Kaneko. .  Ventral medial nucleus neurons send thalamocortical afferents more widely and more preferentially to layer 1 than neurons of the ventral anterior-ventral lateral nuclear complex in the rat. .  Cerebral Cortex25 ( 1 ) 221 - 235   2015Ventral medial nucleus neurons send thalamocortical afferents more widely and more preferentially to layer 1 than neurons of the ventral anterior-ventral lateral nuclear complex in the rat.Reviewed

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  • Hiroyuki Hioki, Shinichi Okamoto, Michiteru Konno, Hiroshi Kameda, Jaerin Sohn, Eriko Kuramoto, Fumino Fujiyama, Takeshi Kaneko. .  Cell-type specific inhibitory inputs to dendritic and somatic components of parvalbumin-expressing neocortical interneuron. .  Journal of Neuroscience33 ( 2 ) 544 - 555   2013.2Cell-type specific inhibitory inputs to dendritic and somatic components of parvalbumin-expressing neocortical interneuron.Reviewed

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  • Sachi Ohno, Eriko Kuramoto, Fumino Fujiyama, Hiroyuki Hioki, Yasuhiro R. Tanaka , Fumino Fujiyama, Takahiro Sonomura, Masanori Uemura, Kazuna Sugiyama, Takeshi Kaneko. .  A morphological analysis of thalamocortical axon fibers of rat posterior thalamic nuclei: A single neuron tracing study with viral vectors. .  Cerebral Cortex22 ( 12 ) 2840 - 2857   2012.12A morphological analysis of thalamocortical axon fibers of rat posterior thalamic nuclei: A single neuron tracing study with viral vectors. Reviewed

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  • Eriko Kuramoto, Fumino Fujiyama, Kouichi C. Nakamura, Yasuhiro Tanaka, Hiroyuki Hioki, Takeshi Kaneko .  Complementary distribution of glutamatergic cerebellar and GABAergic basal ganglia afferents to the rat motor thalamic nuclei. .  European Journal of Neuroscience33 ( 1 ) 95 - 109   2011Complementary distribution of glutamatergic cerebellar and GABAergic basal ganglia afferents to the rat motor thalamic nuclei.Reviewed

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  • Koji Ohira, Takahiro Furuta, Hiroyuki Hioki, Kouichi C. Nakamura, Eriko Kuramoto, Yasuyo Tanaka, Nobuo Funatsu, Keiko Shimizu, Takao Oishi, Motoharu Hayashi, Tsuyoshi Miyakawa, Takeshi Kaneko, Shun Nakamura. .  Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells. .  Nature Neuroscience13 ( 2 ) 173 - 179   2010Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells.Reviewed

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  • Hiroyuki Hioki, Eriko Kuramoto, Michiteru Konno, Hiroshi Kameda, Yasuhiro Takahashi, Takashi Nakano, Kouichi C. Nakmura, Takeshi Kaneko .  High-level transgene expression in neurons by lentivirus with Tet-Off system. .  Neuroscience Research63 ( 2 ) 149 - 154   2009High-level transgene expression in neurons by lentivirus with Tet-Off system.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Eriko Kuramoto, Takahiro Furuta, Kouichi C Nakamura, Tomo Unzai, Hiroyuki Hioki, Takeshi Kaneko .  Two types of thalamocortical projections from the motor thalamic nuclei of the rat: a single neuron tracing study using viral vectors. .  Cerebral Cortex9 ( 9 ) 2065 - 2077   2009Two types of thalamocortical projections from the motor thalamic nuclei of the rat: a single neuron tracing study using viral vectors.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Eriko Kuramoto, Fumino Fujiyama, Tomo Unzai, Kouichi C. Nakamura, Hiroyuki Hioki, Takahiro Furuta, Ryuichi Shigemoto, Francesco Ferraguti, Takeshi Kaneko. .  Metabotropic glutamate receptor 4-immunopositive terminals of medium-sized spiny neurons selectively form synapses with cholinergic interneurons in the rat neostriatum. .  Journal of Comparative Neurology500 ( 5 ) 908 - 922   2007Metabotropic glutamate receptor 4-immunopositive terminals of medium-sized spiny neurons selectively form synapses with cholinergic interneurons in the rat neostriatum.Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Fumino Fujiyama, Eriko Kuramoto, Keiko Okamoto, Hiroyuki Hioki, Takahiro Furuta, Ligang Zhou, Sakashi Nomura, Takeshi Kaneko. .  Presynaptic localization of an AMPA-type glutamate receptor in corticostriatal and thalamostriatal axon terminals. .  European Journal of Neuroscience20 ( 12 ) 3322 - 3330   2004Presynaptic localization of an AMPA-type glutamate receptor in corticostriatal and thalamostriatal axon terminals. Reviewed

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    Language：English   Publishing type：Research paper (scientific journal)  

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MISC

  • 三叉神経節にみられる細胞構築と細胞間相互作用　口腔顔面領域の神経障害性疼痛との関連性(Cytoarchitecture and intercellular interactions in the trigeminal ganglion: Associations with neuropathic pain in the orofacial region)

    Goto Tetsuya, Kuramoto Eriko, Iwai Haruki, Yamanaka Atsushi

    Journal of Oral Biosciences   66 ( 3 )   485 - 490   2024.9

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    Language：English   Publisher：(一社)歯科基礎医学会  

  • A novel system to analyze oral frailty of mice

    Goto T., Kuramoto E., Kitawaki A.

    Aging   15 ( 7 )   2362 - 2363   2023

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    Language：Japanese   Publisher：Aging  

    Oral frailty has been recently proposed as a novel frailty phenotype and defined as a decrease in oral function coexisting with a decline in cognitive and physical functions [1]. Oral frailty is currently attracting attention, especially in relation to cognitive function [2]. Recent clinical studies have found that patients with Alzheimer’s disease (AD) have impaired masticatory function from an early stage of mild cognitive impairment (MCI) [3]

    DOI： 10.18632/aging.204568

    Scopus

    PubMed

  • 【神経回路の可視化-何が見える、どこまで見える】標本で見る　遺伝子工学を用いた神経結合の可視化

    倉本 恵梨子

    Clinical Neuroscience   40 ( 6 )   721 - 725   2022.6

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    Language：Japanese   Publisher：(株)中外医学社  

  • IV. 基底核と小脳：その相違と連関. 4. 運動野での両者の差. Invited

    KURAMOTO, Eriko

    Clinical Neuroscience.   35 ( 1 )   28 - 42   2017.1

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • Novel single-neuron-tracing method using Sindbis viral vectors. Reviewed

    Eriko Kuramoto, Takahiro Furuta, Hiroyuki Hioki, Fumino Fujiyama, Takeshi Kaneko.

    顕微鏡   46 ( 2 )   125 - 131   2011

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Presentations

  • Fukushima Makoto, Kuramoto Eriko, Sendo Ryozo, Iwai Haruki, Yamanaka Atsushi, Sugimura Mitsutaka, Goto Tetsuya   逆行性トレーサーと組織透明化技術の組み合わせによるラット三叉神経節の三次元体部位局在の解明(Three-Dimensional topography of the rat trigeminal ganglion neurons with combination of retrograde labeling and tissue clearing techniques)  

    The Journal of Physiological Sciences  2021.8  (一社)日本生理学会

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    Language：English  

  • 園田 怜美, 倉本 恵梨子, 南 総一郎, 松本 信英, 原 博満, 大八木 保政, 斉藤 貴志, 西道 隆臣, 野口 和行, 後藤 哲哉   神経細胞における選択的オートファジーとAβオリゴマーの動態について  

    老年精神医学雑誌  2022.11  (株)ワールドプランニング

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    Language：Japanese  

  • 原口 みさ子, 城山 優治, 佐藤 正宏, 昌子 浩孝, 宮川 剛, 脇田 愛, 倉本 恵梨子, 奥野 浩行   活動依存的に発現する脂質修飾酵素zdhhc18のin vitro,in vivoにおける機能  

    日本生化学会大会プログラム・講演要旨集  2024.11  (公社)日本生化学会

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    Language：Japanese  

  • 園田 怜美, 倉本 恵梨子, 南 総一郎, 後藤 哲哉, 野口 和行   歯の喪失がアルツハイマー病の進行に影響を与えるメカニズムについて  

    日本歯周病学会会誌  2022.5  (NPO)日本歯周病学会

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  • 新納 彩子, 大野 幸, 富田 和男, 倉本 恵梨子, 中村 渉, 杉村 光隆   急性持続性疼痛の動物モデルを用いた、三叉神経支配領域における痛みの日内変動の解析(Diurnal variation in trigeminal pain sensitivity in mice)  

    Journal of Oral Biosciences Supplement  2021.10  (一社)歯科基礎医学会

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    Language：Japanese  

  • 倉本 恵梨子, 後藤 哲哉   三叉神経系からの入力を受ける視床ニューロンの樹状突起スパインの形態解析  

    Journal of Oral Biosciences Supplement  2024.11  (一社)歯科基礎医学会

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    Language：Japanese  

  • Goto Tetsuya, Kuramoto Eriko, Iwai Haruki, Yamanaka Atsushi   三叉神経節における神経細胞の三次元トポグラフィーとM2マクロファージによる神経保護(Neural mechanisms of pain in the orofacial area　Three-dimensional topography of the neurons and neuroprotection by M2 macrophages in the trigeminal ganglion)  

    Journal of Oral Biosciences Supplement  2023.9  (一社)歯科基礎医学会

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    Language：English  

  • 園田 怜美, 倉本 恵梨子, 松本 信英, 原 博満, 大八木 保政, 野口 和行, 後藤 哲哉   三叉神経中脳路核の神経変性によるアミロイドβオリゴマーの拡散について  

    Dementia Japan  2021.10  (一社)日本認知症学会

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    Language：Japanese  

  • 倉本 恵梨子, 福島 慎, 岩井 治樹, 山中 淳之, 杉村 光隆, 後藤 哲哉   ラット三叉神経節の三次元的な体部位局在と異所性疼痛の関連性についての研究(A study of the relationship between three-dimensional somatotopy of the rat trigeminal ganglion and ectopic pain)  

    Journal of Oral Biosciences Supplement  2021.10  (一社)歯科基礎医学会

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    Language：Japanese  

  • 倉本 恵梨子, 岩井 治樹, 山中 淳之, 後藤 哲哉   マウス三叉神経核から大脳皮質へと至る神経回路の、アデノ随伴ウイルスベクターを用いた解析  

    Journal of Oral Biosciences Supplement  2023.9  (一社)歯科基礎医学会

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    Language：Japanese  

  • 倉本 恵梨子, 後藤 哲哉   マウスモデルを用いたアルツハイマー病に伴う口腔機能低下症の解析システムの開発  

    日本老年歯科医学会総会・学術大会プログラム・抄録集  2023.6  (一社)日本老年歯科医学会

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    Language：Japanese  

  • 後藤 哲哉, 倉本 恵梨子   ブレインサイエンスが拓く、咬合咀嚼の生理学　三叉神経中脳路核の特異性から読み取る、咬合・咀嚼と脳機能の関係について(The relationship between occlusion, mastication, and brain function as interpreted from the specificity of the trigeminal mesencephalic nucleus)  

    Journal of Oral Biosciences Supplement  2022.9  (一社)歯科基礎医学会

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    Language：Japanese  

  • 後藤 哲哉, 倉本 恵梨子, 北脇 綾乃, 河野 博史, 松本 信英, 原 博満, 八木 孝和, 大八木 保政, 岩井 治樹, 山中 淳之   アルツハイマー病モデルマウスを使った口腔フレイル解析システムの開発  

    老年精神医学雑誌  2022.11  (株)ワールドプランニング

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    Language：Japanese  

  • 北脇 綾乃, 倉本 恵梨子, 齋藤 充, 山中 淳之, 岩井 治樹, 後藤 哲哉   アルツハイマー病モデルマウスの中脳路核でみられたアミロイドβとリン酸化タウの沈着と、その咀嚼機能への影響(Neuropathology in the mesencephalic trigeminal nucleus of Alzheimer's disease model mice and its effects on masticatory function)  

    Journal of Oral Biosciences Supplement  2021.10  (一社)歯科基礎医学会

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    Language：Japanese  

  • Kitawaki Ayano, Esaki Harukiyo, Oda Hinano, Katsuyama Kouki, Kinoshita Teruki, Susa Megumi, Taniguchi Masayoshi, Kuramoto Eriko, Saito Mitsuru, Kono Hiroshi, Yagi Takakazu, Matsumoto Shinei, Hara Hiromitsu, Iwai Haruki, Yamanaka Atsushi, Goto Tetsuya   アルツハイマー病モデルマウス3xTg-ADの三叉神経系における病理所見と咀嚼運動についての研究(Pathologies of the trigeminal nervous system, and delayed masticatory rhythm in triple-transgenic mouse model of Alzheimer's disease)  

    The Journal of Physiological Sciences  2021.8  (一社)日本生理学会

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  • 北脇 綾乃, 倉本 恵梨子, 齋藤 充, 河野 博史, 岩井 治樹, 山中 淳之, 後藤 哲哉   アルツハイマーモデルマウス3xTG-ADの三叉神経系における病理所見と咀嚼機能についての研究(Pathologies of the trigeminal nervous system, and delayed masticatory rhythm in triple-transgenic mouse model of Alzheimer's disease)  

    Journal of Oral Biosciences Supplement  2021.10  (一社)歯科基礎医学会

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    Language：Japanese  

  • 倉本 恵梨子, 岩井 治樹, 山中 淳之, 後藤 哲哉   アデノ随伴ウイルスベクターを用いた、マウス三叉神経脊髄路核から視床に投射するニューロンの特異的標識と光活性化(Tracing and photostimulation of projection neurons form the mouse spinal trigeminal nucleus to the thalamus usig adeno-associated virus vectors)  

    Journal of Oral Biosciences Supplement  2022.9  (一社)歯科基礎医学会

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    Language：Japanese  

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Research Projects

  • Elucidation of the mechanism by which aging-induced deterioration of oral function is involved in the development of Alzheimer's disease.

    Grant number：23H03119  2023.4 - 2027.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Grant amount：\18590000 （ Direct Cost: \14300000 、 Indirect Cost：\4290000 ）

  • 行動変容を導く皮質ー皮質下相互作用

    Grant number：22H05162  2022.6 - 2027.3

    日本学術振興会  科学研究費助成事業  学術変革領域研究(A)

    田中 康裕, 高田 美絵子, 倉本 恵梨子

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    Grant amount：\85800000 （ Direct Cost: \66000000 、 Indirect Cost：\19800000 ）

  • 痛みを増強・抑制する２つの視床-皮質回路の、青斑核によるバランシング機構の解明

    Grant number：22K09916  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    倉本 恵梨子, 大野 幸, 後藤 哲哉, 柏谷 英樹

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    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

  • Elucidation of the molecular mechanism by which neurodegeneration caused by tooth loss initiates Alzheimer's disease

    Grant number：20K10296  2020.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

  • 周術期の摂食条件が体内時計に与える影響；遺伝子改変マウスを用いた時間生物学的解析

    Grant number：19K10336  2019.4 - 2023.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    大野 幸, 倉本 恵梨子, 杉村 光隆

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

    近年、生活習慣病をはじめとする多くの疾病は、「体内時計」の不調に一因があるという知見が次々と報告され、この分野の研究が急速に進んでいる。中でも食事が「体内時計」に与える影響は大きく、その仕組みを知りうまく活用することで、人々の健康や疾病対策に大きく寄与するのではないかと期待されている。また、「腹時計」は抽象的な概念ではなく、‘経口’摂取による食事刺激が「腹時計」を介した生体リズムの維持において重要であることも古くから指摘されている。そこで申請者は、食事刺激を受け取る「腹時計」の鍵は‘口’にあるのではないかと考え、周術期の口腔内への侵襲や不規則な食事が「腹時計」機構に不調和を招き、その結果が口腔外科領域においても問題となる術後せん妄などの合併症につながるのではないか？という問いに至った。
    本研究では、時計遺伝子の１つPeriod2にホタルの発光酵素をつけ、時計遺伝子の発現に応じて細胞が光るよう遺伝子改変されたマウス（PER2::LUCノックインマウス）を用い、三叉神経節における時計遺伝子発現のリズムを計測した。次に、別の時計遺伝子であるCry１またはCry２をノックアウトしたPER2::LUCノックインマウスを用いて同様の実験を行い、時計遺伝子の役割についても考察を加えた。また、共同研究によって、三叉神経節の発光についてイメージング画像の解析も行った.さらに、イメージングによってPER2が発現してる部位には局在があることがわかったが、局在について検討するため、免疫染色を追加実験として行う方針であり、現在準備を行っている状況である。今年度は最終年度であり、成果発表に向けて準備をすすめている。

  • "痛み"認知における,高次視床核を介した大脳皮質シンクロ活動の関与と除痛への応用

    Grant number：19K10058  2019.4 - 2023.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    倉本 恵梨子, 大野 幸, 後藤 哲哉, 柏谷 英樹, 杉村 光隆

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

    ”痛み”を感じている人の脳では、視床、そして大脳皮質の体性感覚野と島皮質が、特に活発な活動を示す。体性感覚野が司る識別感覚と、島皮質で処理される不快感という、分割された2種類の情報が再び一つに統合されて、”痛み”の認知を生じると考えられているが、そのメカニズムは未だ不明である。本研究では、情報を統合するメカニズムとして、視床核の一つであるPosterior nuclei (PO, 視床後核群)の、特に尾側部のニューロンが、体性感覚野と島皮質の両方に
    同時に出力し、シンクロ活動を誘発することで、2種類の情報が統合され、”痛み”として認知されるという仮説を検証する。
    昨年度までに、HungaryのProfessor Acsadyと国際共同研究を立ち上げ、本研究で必要となる、光遺伝学・行動解析・電気生理学の技術を融合した実験技術を確立することができた。具体的には、マウスの特定の神経細胞に光活性化チャネルを発現するアデノ随伴ウイルスを感染させ、これを刺激するためのoptic-fiberと、神経活動を記録するための電極の埋入、そして、オンラインでマウスの行動を解析し、特定の行動をしたときに、自動的に光刺激が行われる、closed loop systemを完成させた。
    今年度は、三叉神経脊髄路核ニューロンを光遺伝学により活性化し、痛みの神経経路を刺激したときに、高次視床核である視床後核群が発火しているか、そして、島皮質、第一次体性感覚野、第二次体性感覚野の神経活動が同期しているかを、確認する。これらが確認できた後に、島皮質、第一次体性感覚野、第二次体性感覚野のいずれかを、光遺伝学により抑制することにより、神経活動の非同期化を行い、それにより痛覚関連行動や、不快情動を抑制できるかどうか、検証を行う予定である。

  • Effects of brain-gut peptides on the amelioration of stress-induced abnormalities of maxillofacial function.

    Grant number：18K09838  2018.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Yagi Takakazu

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    Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）

    The purpose of this study was to elucidate some of the mechanisms of the development of bruxism-like movements induced by intracerebroventricular administration of corticosteroid releasing factor (CRF), a stressor, or by physical restraints, focusing on the relationship between stress and the trigeminal nervous system. The parabrachial nucleus of the connecting arm and the locus coeruleus in the region of the trigeminal mesencephalic tract nucleus were positive for anti-c-Fos antibodies. In restraint stress, immunopositive cells were observed around the trigeminal motor nucleus and in the lateral nucleus of the hypothalamus, and double-labeled cells of c-Fos and orexin were observed. These results suggest that the connecting arm antinuclear nucleus, locus coeruleus, and orexin cells may be potentially contributing to bruxism-like behavior.

  • Cortical local circuits for multi-area interconnectivity and parallel processing

    Grant number：17H06311  2017.6 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

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    Grant amount：\113750000 （ Direct Cost: \87500000 、 Indirect Cost：\26250000 ）

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Teaching Experience

  • 神経解剖学

    Institution：京都大学

  • 組織学

    Institution：鹿児島大学