2021/06/01 更新

写真a

ヨコヤマ セイヤ
横山 勢也
Seiya Yokoyama
所属
医歯学域医学系 医歯学総合研究科 先進治療科学専攻 腫瘍学講座 助教
職名
助教

学位

  • 博士(農学) ( 2009年9月   鹿児島大学 )

研究キーワード

  • エピジェネティック制御

  • DNA メチル化

  • 機械学習

  • 膵臓癌

学歴

  • 鹿児島大学   応用生命科学専攻 生物機能化学連合講座

    2004年4月 - 2008年3月

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    国名: 日本国

  • 鹿児島大学   生物資源化学科

    1997年4月 - 2002年3月

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    国名: 日本国

経歴

  • 鹿児島大学   医歯学域医学系 医歯学総合研究科 先進治療科学専攻 腫瘍学講座 病理学分野   助教

    2017年4月 - 現在

  • 鹿児島大学   医歯学域医学系 医歯学総合研究科 先進治療科学専攻 腫瘍学講座 人体がん病理学分野   助教

    2012年4月 - 2017年3月

  • 鹿児島大学   医歯学域医学系 医歯学総合研究科 先進治療科学専攻 腫瘍学講座 人体がん病理学分野   研究員

    2009年4月 - 2012年3月

所属学協会

  • 日本癌学会

    2009年10月 - 現在

  • 日本病理学会

    2009年10月 - 現在

留学歴

  • 2015年04月01日 - 2017年03月31日   ネブラスカ大学メディカルセンター   博士研究員

 

論文

  • Seiya Yokoyama, Taiji Hamada, Michiyo Higashi, Kei Matsuo, Kosei Maemura, Hiroshi Kurahara, Michiko Horinouchi, Tsubasa Hiraki, Tomoyuki Sugimoto, Toshiaki Akahane, Suguru Yonezawa, Marko Kornmann, Surinder K Batra, Michael A Hollingsworth, Akihide Tanimoto .  Predicted Prognosis of Patients with Pancreatic Cancer by Machine Learning .  Clinical Cancer Research 26 ( 10 ) 2411 - 2421   2020年

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AACR  

    Abstract
    Purpose: Pancreatic cancer remains a disease of high mortality despite advanced diagnostic techniques. Mucins (MUC) play crucial roles in carcinogenesis and tumor invasion in pancreatic cancers. MUC1 and MUC4 expression are related to the aggressive behavior of human neoplasms and a poor patient outcome. In contrast, MUC2 is a tumor suppressor, and we have previously reported that MUC2 is a favorable prognostic factor in pancreatic neoplasia. This study investigates whether the methylation status of three mucin genes from postoperative tissue specimens from patients with pancreatic neoplasms could serve as a predictive biomarker for outcome after surgery.

    Experimental Design: We evaluated the methylation status of MUC1, MUC2, and MUC4 promoter regions in pancreatic tissue samples from 191 patients with various pancreatic lesions using methylation-specific electrophoresis. Then, integrating these results and clinicopathologic features, we used support vector machine-, neural network-, and multinomial-based methods to develop a prognostic classifier.

    Results: Significant differences were identified between the positive- and negative-prediction classifiers of patients in 5-year overall survival (OS) in the cross-validation test. Multivariate analysis revealed that these prognostic classifiers were independent prognostic factors analyzed by not only neoplastic tissues but also nonneoplastic tissues. These classifiers had higher predictive accuracy for OS than tumor size, lymph node metastasis, distant metastasis, and age and can complement the prognostic value of the TNM staging system.

    Conclusions: Analysis of epigenetic changes in mucin genes may be of diagnostic utility and one of the prognostic predictors for patients with pancreatic ductal adenocarcinoma.

    DOI: 10.1158/1078-0432.CCR-19-1247

  • Toshiaki Akahane, Ikumi Kitazono, Shintaro Yanazume, Masaki Kamio, Shinichi Togami, Ippei Sakamoto, Sachio Nohara, Seiya Yokoyama, Hiroaki Kobayashi, Tsubasa Hiraki, Shinsuke Suzuki, Shinichi Ueno & Akihide Tanimoto .  Next-generation sequencing analysis of endometrial screening liquid-based cytology specimens: a comparative study to tissue specimens .  BioMed Central13 ( 1 ) 1 - 12   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元: BMC Medical Genomics  

    Abstract
    Background
    Liquid-based cytology (LBC) is now a widely used method for cytologic screening and cancer diagnosis. Since the cells are fixed with alcohol-based fixatives, and the specimens are stored in a liquid condition, LBC specimens are suitable for genetic analyses.

    Methods
    Here, we established a small cancer gene panel, including 60 genes and 17 microsatellite markers for next-generation sequencing, and applied to residual LBC specimens obtained by endometrial cancer screening to compare with corresponding formalin-fixed paraffin-embedded (FFPE) tissues.

    Results
    A total of 49 FFPE and LBC specimens (n = 24) were analyzed, revealing characteristic mutations for endometrial cancer, including PTEN, CTNNB1, PIK3CA, and PIK3R1 mutations. Eight cases had higher scores for both tumor mutation burden (TMB) and microsatellite instability (MSI), which agree with defective mismatch repair (MMR) protein expression. Paired endometrial LBC, and biopsied and/or resected FFPE tissues from 7 cases, presented almost identical mutations, TMB, and MSI profiles in all cases.

    Conclusion
    These findings demonstrate that our ad hoc cancer gene panel enabled the detection of therapeutically actionable gene mutations in endometrial LBC and FFPE specimens. Endometrial cancer LBC specimens offer an alternative and affordable source of molecular testing materials.

    DOI: 10.1186/s12920-020-00753-6

  • Ikumi Kitazono, Taiji Hamada, Takuya Yoshimura, Mari Kirishima, Seiya Yokoyama, Toshiaki Akahane & Akihide Tanimoto .  PCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells .  Laboratory Investigation   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nature Publishing Group  

    Abstract
    Purkinje cell protein 4/peptide 19 (PCP4/PEP19) is 7.6 kDa peptide originally found in Purkinje cells. PCP4/PEP19 is a differentiation maker of Purkinje cells, where it functions as an antiapoptotic factor. Cerebral neuronal cells also express PCP4/PEP19, which may be related to neuronal cell survival. However, evidence suggests that PCP4/PEP19 may also be involved in neuronal differentiation. Here, we investigated the effects of PCP4/PEP19 expression on neuronal differentiation by analyzing neurite outgrowth, and expression of neuronal differentiation markers in cultured human neuroblastoma M17 cells. When PCP4/PEP19 expression was reduced by siRNA-mediated knockdown, neurite outgrowth was significantly increased. Among many differentiation markers tested, expression of NeuroD1 was increased, while that of Ascl1 was decreased upon PCP4/PEP19 knockdown. Furthermore, luciferase reporter assays revealed that PCP4/PEP19 knockdown upregulated NeuroD1 and downregulated Ascl1 expression, at the transcriptional level. These results suggest a new function of PCP4/PEP19, which suppresses neurite outgrowth and neuronal differentiation through the regulation of NeuroD1 and Ascl1 expression in M17 cells. Furthermore, immunohistochemical studies showed that PCP4/PEP19 localizes in the nuclei of human neuroblastoma cells. Therefore, PCP4/PEP19 may also be an intranuclear negative regulator of neuronal differentiation and may thus be a potential therapeutic target to promote cellular differentiation in human neuroblastoma.

    DOI: 10.1038/s41374-020-0462-z

  • Guo X, Noguchi H, Ishii N, Homma T, Hamada T, Hiraki T, Zhang J, Matsuo K, Yokoyama S, Ishibashi H, Fukushige T, Kanekura T, Fujii J, Uramoto H, Tanimoto A, Yamada S .  The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular Carcinoma. .  Antioxidants & Redox Signaling   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Aims: Peroxiredoxin 4 (PRDX4) is a member of the peroxiredoxin family of antioxidant enzymes. Previously, we reported that PRDX4 can restrain the initiation and progression of non-alcoholic steatohepatitis by reducing local and systemic reactive oxygen species (ROS) levels. Oxidative stress is recognized as a key factor in hepatocarcinogenesis, and a high ROS level has also been found in hepatocellular carcinoma (HCC). Here, our aim is to investigate roles of PRDX4 in the initiation and progression of HCC. Results: In this study, for hepatocarcinogenesis, wild-type (WT), PRDX4 knockout (PRDX4-/y) and human PRDX4 transgenic (hPRDX4+/+) mice were given a weekly intraperitoneal injection of diethylnitrosamine (DEN) for 25 weeks. The HCC incidence was higher in PRDX4-/y mice than in WT or hPRDX4+/+ mice. Intrahepatic and circulating oxidative stress and inflammatory cell infiltration in the liver were obviously decreased in hPRDX4+/+ mice, compared to WT mice. Furthermore, in our cohort study, human HCC specimens with low expression of PRDX4 had higher ROS levels and a highly malignant phenotype, which was associated with a reduced overall survival, compared to those with high PRDX4 expression. However, in human HCC cell lines, PRDX4 knockdown led to a rapidly increased intracellular ROS level and suppressed cell proliferation, inducing cell death. Innovation and Conclusion: Our results clearly indicate that PRDX4 has an inhibitory effect in the initiation of HCC but a dual (inhibitory or promoting) role in the progression of HCC, suggesting the potential utility of PRDX4 activators or inhibitors as therapy for different stages and phenotypes of HCC.

    DOI: doi.org/10.1089/ars.2017.7426

  • Atsushi Matsuda, Michiyo Higashi, Tomomi Nakagawa, Seiya Yokoyama, Atsushi Kuno, Suguru Yonezawa, Hisashi Narimatsu .  Assessment of tumor characteristics based on glycoform analysis of membrane-tethered MUC1. .  Laboratory Investigation   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nature Publishing Group  

    Clinical tissue specimens are useful for pathological diagnosis, which is, in some cases, supported by visualization of biomolecule localization. In general, diagnostic specificity in molecular pathology is increased by the acquisition of a probe to distinguish the modification of isomers. Although glycosylation is one of the candidate modifications in a protein, comparative glycan analysis of disease-associated proteins derived from a single tissue section is still challenging because of the lack of analytical sensitivity. Here we demonstrate a possible method for differential glycoform analysis of an endogenous tumor-associated glycoprotein MUC1 by an antibody-overlay lectin microarray. Tissue sections (5 μm thick) of patients with cholangiocarcinoma (CCA; n=21) and pancreatic ductal adenocarcinoma (PDAC; n=50) were stained with an anti-MUC1 antibody MY.1E12 that was established as a monoclonal antibody recognizing an MUC1 glycosylation isoform with a sialyl-core 1 structure (NeuAcα2-3galactosyl β1-3-N-acetylgalactosamine). MY.1E12-positive tissue areas (2.5 mm2) were selectively dissected with a laser capture microdissection procedure. The membrane MUC1 was enriched by immunoprecipitation with MY.1E12 and subjected to lectin microarray analysis. Even though the reactivities of MY.1E12 between CCA and PDAC were similar, the lectin-binding patterns varied. We found Maackia amurensis leukoagglutinin and pokeweed lectin distinguished MY.1E12-reactive MUC1 of CCA from that of PDAC. Moreover, MUC1 with M. amurensis hemagglutinin (MAH) reactivity potentially reflected the degree of malignancy. These results were confirmed with MAH-MY.1E12 double fluorescent immunostaining. These glycan changes on MUC1 were detected with high sensitivity owing to the cluster effect of immobilized lectins on a tandem repeat peptide antigen covered with highly dense glycosylation such as mucin. Our approach provides the information to investigate novel glycodynamics in biology, for example, glycoalteration, as well as diseases related to not only MUC1 but also other membrane proteins.

    DOI: 10.1038/labinvest.2017.53

    PubMed

  • Seiya Yokoyama, Michiyo Higashi, Hideaki Tsutsumida, Jouji Wakimoto, Tomofumi Hamada, Edwin Wiest, Kei Matsuo, Ikumi Kitazono, Yuko Goto, Xin Guo, Taiji Hamada, Sohsuke Yamada, Tsubasa Hiraki, Suguru Yonezawa, Surinder K Batra, Michael A Hollingsworth, Akihide Tanimoto .  TET1-mediated DNA hypomethylation regulates the expression of MUC4 in lung cancer .  Genes Cancer8 ( 3-4 ) 517 - 527   2017年

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:impact journal  

    Lung cancer remains a disease of high mortality, despite advanced diagnostic techniques. Mucins (MUC) play crucial roles in carcinogenesis and tumor invasion in lung neoplasms. Our immunohistochemistry (IHC) studies have shown that high MUC4 expression correlates with a poor outcome. We have also shown that the expression of several mucin genes in cancer cell lines is regulated by DNA methylation. We evaluated the expression level of MUC4, mRNA and several DNA hypomethylation factors in lung tissue samples from 33 patients with various lung lesions. The results indicated that the DNA methylation status of MUC4 matched the expression level of mRNA. In addition, the TET1 (Ten-Eleven Translocation) mRNA showed a significant correlation with the status of DNA methylation of MUC4. Furthermore, the treatment of a lung cancer cell line with TET1 siRNA caused a reduction in MUC4 mRNA expression. Thus, we suggest that TET1 mediated DNA hypomethylation plays a key role in the expression of MUC4. This is the first report that TET1 mediated DNA hypomethylation regulates the expression of MUC4 in lung cancer. The analysis of these epigenetic changes may be useful for diagnosing carcinogenic risk.

    DOI: 10.18632/genesandcancer.139.

    PubMed

  • Tsubasa Hiraki, Sohsuke Yamada, Michiyo Higashi, Kazuhito Hatanaka, Seiya Yokoyama, Ikumi Kitazono, Yuko Goto, Mari Kirishima, Surinder K. Batra, Suguru Yonezawa, Akihide Tanimoto .  Immunohistochemical expression of mucin antigens in gallbladder adenocarcinoma: MUC1-positive and MUC2-negative expression Is associated with vessel invasion and shortened survival. .  Histology and histopathology   2016年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Summary. Mucins play pivotal roles in influencing cancer biology, for example affecting carcinoma invasion, aggressiveness and/or metastatic potential. Our aim is to investigate the significance of expression profiles of two mucins in particular, MUC1 and MUC2, their correlations with various clinicopathological features, and prognosis in gallbladder adenocarcinoma (GBAC). We performed immunohistochemistry from patients with surgically resected GBAC, using antibodies against mucin core proteins MUC1/DF3 and MUC2/Ccp58 in 81 paraffin-embedded tumor samples. MUC1 or MUC2 expression was considered to be high when >=20% or 10% of the GBAC cells showed positive staining, respectively. High MUC1 expression was revealed to have a significant relationship to the presence of pathologically lymphatic and vascular invasion, and regional lymph node metastasis. By contrast, high MUC2 expression showed a significant correlation with pathologically perineural invasion, T stage >=3, and post-operative recurrence. Moreover, MUC1 showed significantly positive co-expression and potentially complementary correlations with MUC2. Multivariate analyses demonstrated that the high MUC1 expression group had significantly shorter disease-specific survival times. However, the combination of both high MUC1 and MUC2 expression did not predict worse outcome in GBACs. Therefore, although each mucin has a somewhat important role in the pathogenesis of GBAC progression, MUC1 can independently predict vessel invasion and poor prognosis in patients with GBAC. The detection of MUC1 might well offer a useful parameter for providing clinical management and treatment against postsurgical GBACs.

    DOI: 10.14670/HH-11-824

  • Seiya Yokoyama, Michiyo Higashi, Sho Kitamoto, Monika Oeldorf, Uwe Knippschild, Marko Kornmann, Kosei Maemura, Hiroshi Kurahara, Edwin Wiest, Tomofumi Hamada, Ikumi Kitazono, Yuko Goto, Takashi Tasaki, Tsubasa Hiraki, Kazuhito Hatanaka, Yuko Mataki, Hiroki Taguchi, Shinichi Hashimoto, Surinder K. Batra, Akihide Tanimoto, Suguru Yonezawa, Michael A. Hollingsworth .  Aberrant methylation of MUC1 and MUC4 promoters are potential prognostic biomarkers for pancreatic ductal adenocarcinomas. .  Oncotarget   2016年6月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Pancreatic cancer is still a disease of high mortality despite availability of diagnostic techniques. Mucins (MUC) play crucial roles in carcinogenesis and tumor invasion in pancreatic neoplasms. MUC1 and MUC4 are high molecular weight transmembrane mucins. These are overexpressed in many carcinomas, and high expression of these molecules is a risk factor associated with poor prognosis. We evaluated the methylation status of MUC1 and MUC4 promoter regions in pancreatic tissue samples from 169 patients with various pancreatic lesions by the methylation specific electrophoresis (MSE) method. These results were compared with expression of MUC1 and MUC4, several DNA methylation/demethylation factors (e.g. ten-eleven translocation or TET, and activation-induced cytidine deaminase or AID) and CAIX (carbonic anhydrase IX, as a hypoxia biomarker). These results were also analyzed with clinicopathological features including time of overall survival of PDAC patients. We show that the DNA methylation status of the promoters of MUC1 and MUC4 in pancreatic tissue correlates with the expression of MUC1 and MUC4 mRNA. In addition, the expression of several DNA methylation/demethylation factors show a significant correlation with MUC1 and MUC4 methylation status. Furthermore, CAIX expression significantly correlates with the expression of MUC1 and MUC4. Interestingly, our results indicate that low methylation of MUC1 and/or MUC4 promoters correlates with decreased overall survival. This is the first report to show a relationship between MUC1 and/or MUC4 methylation status and prognosis. Analysis of epigenetic changes in mucin genes may be of diagnostic utility and one of the prognostic predictors for patients with PDAC.

    DOI: 10.18632/oncotarget.9924

    その他リンク: http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=9924&pubmed-linkout=1

  • Higashi, Michiyo; Yokoyama, Seiya; Yamamoto, Takafumi; Goto, Yuko; Kitazono, Ikumi; Hiraki, Tsubasa; Taguchi, Hiroki; Hashimoto, Shinichi; Fukukura, Yoshihiko; Koriyama, Chihaya; Mataki, Yuko; Maemura, Kosei; Shinchi, Hiroyuki; Jain, Maneesh; Batra, Surinder K.; Yonezawa, Suguru .  Mucin Expression in Endoscopic Ultrasound-Guided Fine-Needle Aspiration Specimens Is a Useful Prognostic Factor in Pancreatic Ductal Adenocarcinoma .  Pancreas44 ( 5 ) 728 - 734   2015年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  



    Objectives: The aim of this study was to further examine the utility of mucin (MUC) expression profiles as prognostic factors in pancreatic ductal adenocarcinoma (PDAC).

    Methods: Mucin expression was examined by immunohistochemistry analysis in endoscopic ultrasound-guided fine-needle aspiration specimens obtained from 114 patients with PDAC. The rate of expression of each MUC was compared with clinicopathologic features.

    Results: The expression rates of MUCs in cancer lesions were MUC1, 87.7%; MUC2, 0.8%; MUC4, 93.0%; MUC5AC, 78.9%; MUC6, 24.6%; and MUC16, 67.5%. MUC1 and MUC4 were positive, and MUC2 was negative in most PDACs. Patients with advanced stage of PDAC with MUC5AC expression had a significantly better outcome than those who were MUC5AC-negative (P = 0.002). With increasing clinical stage, total MUC6 expression decreased (P for trend = 0.001) and MUC16 cytoplasmic expression increased (P for trend = 0.02). The prognosis of patients with MUC16 cytoplasmic expression was significantly poorer than those without this expression. Multivariate survival analysis revealed that MUC16 cytoplasmic expression was a significant independent predictor of a poor prognosis after adjusting for the effects of other prognostic factors (P = 0.002).

    Conclusions: Mucin expression profiles in ultrasound-guided fine-needle aspiration specimens have excellent diagnostic utility and are useful predictors of outcome in patients with PDAC.

    DOI: 10.1097/MPA.0000000000000362

    その他リンク: http://journals.lww.com/pancreasjournal/Abstract/2015/07000/Mucin_Expression_in_Endoscopic_Ultrasound_Guided.6.aspx

  • Yokoyama S, Kitamoto S, Higashi M, Goto Y, Hara T, Ikebe D, Yamaguchi T, Arisaka Y, Niihara T, Nishimata H, Tanaka S, Takaori K, Batra SK, Yonezawa S. .  Diagnosis of pancreatic neoplasms using a novel method of DNA methylation analysis of mucin expression in pancreatic juice. .  PLoS One9 ( 4 ) e93760 - doi: 10.1371/journal.pone.0093760   2014年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Shibahara H, Higashi M, Koriyama C, Yokoyama S, Kitazono I, Kurumiya Y, Narita M, Kuze S, Kyokane T, Mita S, Arai T, Kato T, Yuasa N, Yamaguchi R, Kubota H, Suzuki H, Baba S, Rousseau K, Batra SK, Yonezawa S. .  Pathobiological Implications of Mucin (MUC) Expression in the Outcome of Small Bowel Cancer. .  PLoS One9 ( 4 ) e86111 - doi: 10.1371/journal.pone.0086111.   2014年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Hiraki T, Higashi M, Goto Y, Kitazono I, Yokoyama S, Iuchi H, Nagano H, Tanimoto A, Yonezawa S. .  A rare case of internal jugular vein aneurysm with massive hemorrhage in neurofibromatosis type 1. .  Cardiovasc Pathol.published online. ( doi: 10.1016/j.carpath.2014.02.001. )   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Kitazono I, Higashi M, Kitamoto S, Yokoyama S, Horinouchi M, Osako M, Shimizu T, Tabata M, Batra SK, Goto M, Yonezawa S. .  Expression of MUC4 mucin is observed mainly in the intestinal type of intraductal papillary mucinous neoplasm of the pancreas. .  Pancreas42 ( 7 ) 1120 - 1128   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Sho Kitamoto, Seiya Yokoyama, Michiyo Higashi, Norishige Yamada, Sonshin Takao, Suguru Yonezawa .  MUC1 enhances hypoxia-driven angiogenesis through the regulation of multiple proangiogenic factors. .  Oncogene32 ( 39 ) 4614 - 4621   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Matsuno YK, Dong W, Yokoyama S, Yonezawa S, Narimatsu H, Kameyama A. .  Identification of mucins by using a method involving a combination of on-membrane chemical deglycosylation and immunostaining. .  J. Immunol. Methods.394 ( 12 ) 125 - 130   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Seiya Yokoyama, Sho Kitamoto, Norishige Yamada, Izumi Houjou, Tamotsu Sugai, Shin-ichi Nakamura, Yoshifumi Arisaka, Kyoichi Takaori, Michiyo Higashi and Suguru Yonezawa .  The application of Methylation specific electrophoresis (MSE) to DNA methylation analysis of the 5' CpG island of mucin in cancer cells .  BMC cancer12 ( 67 )   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Higashi M, Yamada N, Yokoyama S, Kitamoto S, Tabata K, Koriyama C, Batra SK, Yonezawa S .  Pathobiological Implications of Expression of MUC16/CA125 in Intrahepatic Cholangiocarcinoma-Mass Forming type. .  Pathobiology   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Yonezawa S, Kitajima S, Higashi M, Osako M, Horinouchi M, Yokoyama S, Kitamoto S, Yamada N, Tamura K, Shimizu T, Tabata M, Goto M .  A novel anti-MUC1 antibody against the MUC1 cytoplasmic tail domain: use in sensitive identification of poorly differentiated cells in adenocarcinoma of the stomach .  Gastric Cancer   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Nagata S, Hamada T, Yamada N, Yokoyama S, Kitamoto S, Kanmura Y, Nomura M, Kamikawa Y, Yonezawa S, Sugihara K .  Aberrant DNA Methylation of Tumor-related Genes in Oral Rinse is a Promising Marker for Noninvasive Detection/Screening of Oral Squamous Cell Carcinoma. .  Cancer   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Sho Kitamoto, Seiya Yokoyama, Michiyo Higashi, Norishige Yamada, Shyuichiro Matsubara, Sonshin Takao, Surinder K. Batra, Suguru Yonezawa .  Expression of MUC17 Is Regulated by HIF1α-mediated Hypoxic Responses and Requires a Methylation-free Hypoxia Responsible Element in Pancreatic Cancer. .  PLoS ONE7 ( 9 ) e44108 - doi: 10.1371/journal.pone.0044108.   2012年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Tamura Y, Higashi M, Kitamoto S, Yokoyama S, Osako M, Horinouchi M, Shimizu T, Tabata M, Batra SK, Goto M, Yonezawa S. .  MUC4 and MUC1 expression in adenocarcinoma of the stomach correlates with vessel invasion and lymph node metastasis: an immunohistochemical study of early gastric cancer. .  PLoS One7 ( 11 ) e49251 - doi: 10.1371/journal.pone.0049251.   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Kitamoto S, Yamada N, Yokoyama S, Houjou I, Higashi M, Goto M, Batra SK, Yonezawa S. .  DNA methylation and histone H3-K9 modifications contribute to MUC17 expression. .  Glycobiology21 ( 2 ) 247 - 256   2011年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Matsuno Y, Dong W, Yokoyama S, Yonezawa S, Narimatsu H, Kameyama A .  Improved method for immunostaining of mucin separated by supported molecular matrix electrophoresis by optimizing the matrix composition and fixation procedure. .  Electrophoresis32 ( 14 ) 1829 - 1836   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Kitamoto S, Yamada N, Yokoyama S, Houjou I, Higashi M, Yonezawa S .  Promoter hypomethylation contributes to the expression of MUC3A in cancer cells .  Biochem Biophys Res Commun397 ( 2 ) 333 - 339   2010年

    査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Yamada N, Nishida Y, Yokoyama S, Tsutsumida H, Houjou I, Kitamoto S, Goto M, Higashi M, Yonezawa S .  Expression of MUC5AC, an early marker of pancreatobiliary cancer, is regulated by DNA methylation in the distal promoter region in cancer cells. .  J Hepatobiliary Pancreat Sci17 ( 6 ) 844 - 854   2010年

    査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Yokoyama S, Iida Y, Kawasaki Y, Minami Y, Watanabe K, Yagi F .  The chitin-binding capability of Cy-AMP1 from cycad is essential to antifungal activity. .  J Pept Sci15 ( 7 ) 492 - 497   2009年

    査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Yokoyama S, Kato K, Koba A, Minami Y, Watanabe K, Yagi F .  Purification, characterization, and sequencing of antimicrobial peptides, Cy-AMP1, Cy-AMP2, and Cy-AMP3, from the Cycad (Cycas revoluta) seeds. .  Peptides29 ( 12 ) 2110 - 2117   2008年

    査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

▼全件表示

書籍等出版物

  • 新規DNAメチル化解析法による新しい予後マーカーの開発 (特集 革新的ライフサイエンスの展望)

    横山勢也、東美千代( 担当: 共著)

    化學工業  2016年 

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    記述言語:日本語 著書種別:一般書・啓蒙書

  • 新規DNAメチル化解析法の開発と応用

    横山勢也、北本祥、山田宗茂、東美智代、米澤傑( 担当: 共著)

    化學工業  2015年 

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    記述言語:日本語 著書種別:一般書・啓蒙書

  • 第2部 病理診断医になじみのある疾患関連分子「MUC(腫瘍等)解説編・診断編」

    米澤 傑、山田宗茂、横山勢也、北本 祥、田畑和宏、東美智代( 担当: 共著)

    病理と臨床 2011年臨時増刊号 病理診断に役立つ分子生物学  2011年 

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    記述言語:日本語 著書種別:学術書

  • MUC2 (mucin 2, oligomeric mucus/gel-forming)

    35. Yonezawa S, Yamada N, Yokoyama S, Kitamoto S, Higashi M, Goto M( 担当: 共著)

    Atlas of Genetics and Cytogenetics in Oncology and Haematology  2010年 

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    記述言語:英語

MISC

  • 膵癌におけるメチル化によるムチン発現調節

    横山勢也、東美智代、谷本昭英、米澤傑

    肝胆膵76 ( 5 )   2018年5月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)   出版者・発行元:アークメディア  

  • 特集「COPDを改めて問う」エピジェネティクスにおけるムチン発現制御. 査読

    米澤傑, 横山勢也 , 山田宗茂 , 北本祥, 東美智代

    THE LUNG perspectives22 ( 2 ) 77 - 82   2014年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

    さまざまなヒト腫瘍において, MUC1 (汎上皮性膜結合ムチン)は予後不良因子であり, MUC2 (腸型分泌ムチン)は予後良好因子である. また, MUC4 (気管支型膜結合ムチン)高発現はさらなる予後不良に関連している. これらのムチンの遺伝子発現には, DNAメチル化とヒストン修飾というエピジェネティクスが関連している. われわれは, ヒトサンプルのような夾雑な検体の解析が可能な新規DNAメチル化解析法「methylation specific electrophoresis (MSE)」を開発し, 膵液内のMUC1・MUC2・MUC4をMSEで解析することにより, 高い感度と精度をもって, 診断の難しい膵腫瘍を早期に正確に診断するシステムを構築した. 現在, 肺癌におけるムチン発現の研究をベースとして, ヒト肺癌組織におけるMUC1とMUC4の「MSE」による解析が進んでおり, 喀痰や気管支肺胞洗浄液を用いて, ムチンのエピジェネティクスを応用した肺癌早期診断の扉が開かれようとしている.

  • 特集:粘液産生性胆道系腫瘍の再出発-エビデンスとしての画像と病理 「胆道系腫瘍における粘液産生の組織学的側面」. 査読

    東美智代、 柴原弘明、後藤優子、平木 翼、横山勢也、米澤 傑

    胆と膵.34 ( 5 ) 353 - 358   2013年5月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 特集 胆膵病理II:胆膵共通のトピックス【胆膵共通疾患とトピックス】膵胆道腫瘍におけるMUC発現. 査読

    米澤 傑、東美智代、横山勢也、後藤優子、北島信一.

    病理と臨床.31 ( 4 ) 399 - 408   2013年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 胆管内乳頭状腫瘍(IPNB):粘液,MUC発現からのアプローチ. 査読

    東美智代、横山勢也、北本 祥、米澤 傑.

    肝胆膵65 ( 3 ) 487 - 494   2012年9月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 癌におけるムチンの臨床病理学的意義および診断への応用 -難治性膵胆管癌の早期診断に向けて-. 査読

    米澤 傑、東美智代、横山勢也、北本 祥、山田宗茂.

    MEDICAL TECHNOLOGY40 ( 4 ) 419 - 425   2012年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Mucins in human neoplasms: Clinical pathology, gene expression and diagnostic application. 査読

    Yonezawa S, Higashi M, Yamada N, Yokoyama S, Kitamoto S, Kitajima S, Goto M

    Pathol Int61 ( 12 ) 697 - 716   2011年

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Epigenetic regulation of mucin genes in human cancers. 査読

    Yamada N, Kitamoto S, Yokoyama S, Hamada T, Goto M, Tsutsumida H, Higashi M, Yonezawa S

    Clinical Epigenetics2 ( 2 ) 85 - 96   2011年

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • IPMNの組織亜型と分化発育. 査読

    米澤 傑、東美智代、山田宗茂、北本 祥、横山勢也、有坂好史、高折恭一.

    肝胆膵61 ( 3 ) 343 - 358   2010年9月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 腫瘍発生と化生・粘液形質 査読

    東 美智代、田畑和宏、横山勢也、山田宗茂、米澤 傑

    肝胆膵62 ( 1 ) 29 - 38   2010年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • MUC2 (mucin 2, oligomeric mucus/gel-forming) 査読

    Yonezawa S, Yamada N, Yokoyama S, Kitamoto S, Higashi M, Goto M

    Atlas of Genetics and Cytogenetics in Oncology and Haematology   2010年

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • Significance of mucin expression in pancreatobiliary neoplasms 査読

    Yonezawa S, Higashi M, Yamada N, Yokoyama S, Goto M

    J Hepatobiliary Pancreat Sci17 ( 2 ) 108 - 124   2010年

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 分子マトリクス電気泳動法を利用するバイオマーカー探索ストラテジーの構築

    松野裕樹、董偉傑、横山勢也、米澤傑、成松久、亀山昭彦

    生物物理化学54   37   2010年

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

▼全件表示

共同研究・競争的資金等の研究

  • 高感度DNAメチル化解析法を用いた胆管がん早期診断法構築に向けた基礎的研究

    2018年04月 - 2021年03月

    科学研究費補助金  基盤研究(C)

    横山勢也、東美智代、谷本昭英

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  • 膵腫瘍悪性度診断モデルの予測力評価と早期診断への応用 研究課題

    2015年04月 - 2018年03月

    科学研究費補助金  若手研究(B)

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  • 肺がんを標的とした早期診断・治療応用を目指した基礎的研究

    2012年04月 - 2014年03月

    科学研究費補助金  若手研究(B)

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