研究者詳細 - 清水　圭一

論文 - 清水　圭一

分割表示 19 件中 1 - 19 件目／全件表示 >>

Xiuren Liu, Chihiro Hirata, Ton Phuc Huynh, Fumio Hashimoto and Keiichi Shimizu . Development of a Synchronized Germination System and Evaluation of Cotyledon Age and Selection Agents on the Large-Scale Transformation of Model Tomato Cultivar ‘Micro-Tom’ for Functional Genomics . The Horticulture Journalin press 2025年査読国際共著

T.P. Huynh, C. Motoyama, H. Oshima, N. Kozai, F. Hashimoto, K. Shimizu . Functional analysis of flavonoid 3’,5’-hydroxylase gene using transgenic Japanese morning glory (Ipomoea nil) . Acta Horticulturae1404 861 - 866 2024年査読

Yuka Takatori, Keiichi Shimizu, Daraluck Yauwapaksopon, Yu Nakamura, Hiroshi Oshima, Fumio Hashimoto . Flavonoid 3',5'-Hydroxylase (F3'5'H) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] . The Horticulture Journal91 ( 2 ) 229 - 239 2022年4月査読国際共著

濱田延枝，田浦一成，野村哲也，中野八伯，橋本文雄，清水圭一，朴炳宰，遠城道雄 . トルコギキョウの鹿児島大学オリジナル品種作出をめざした育成系統の生育および切り花品質調査 . 鹿児島大学農学部農場研究報告41 1 - 19 2020年

Atsushi Hoshino, Takayuki Mizuno, Keiichi Shimizu, Shoko Mori, Sachiko Fukada-Tanaka, Kazuhiko Furukawa, Kanako Ishiguro, Yoshikazu Tanaka, Shigeru Iida . Generation of Yellow Flowers of the Japanese Morning Glory by Engineering Its Flavonoid Biosynthetic Pathway toward Aurones . Plant and Cell Physiology28 ( 8 ) 1871 - 1879 2019年5月査読

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Oxford University Press

Wild-type plants of the Japanese morning glory (Ipomoea nil) produce blue flowers that accumulate anthocyanin pigments, whereas its mutant cultivars show wide range flower color such as red, magenta and white. However, I. nil lacks yellow color varieties even though yellow flowers were curiously described in words and woodblocks printed in the 19th century. Such yellow flowers have been regarded as ‘phantom morning glories’, and their production has not been achieved despite efforts by breeders of I. nil. The chalcone isomerase (CHI) mutants (including line 54Y) bloom very pale yellow or cream-colored flowers conferred by the accumulation of 2′, 4′, 6′, 4-tetrahydoroxychalcone (THC) 2′-O-glucoside. To produce yellow phantom morning glories, we introduced two snapdragon (Antirrhinum majus) genes to the 54Y line by encoding aureusidin synthase (AmAS1) and chalcone 4′-O-glucosyltransferase (Am4′CGT), which are necessary for the accumulation of aureusidin 6-O-glucoside and yellow coloration in A. majus. The transgenic plants expressing both genes exhibit yellow flowers, a character sought for many years. The flower petals of the transgenic plants contained aureusidin 6-O-glucoside, as well as a reduced amount of THC 2′-O-glucoside. In addition, we identified a novel aurone compound, aureusidin 6-O-(6″-O-malonyl)-glucoside, in the yellow petals. A combination of the coexpression of AmAS1 and Am4′CGT and suppression of CHI is an effective strategy for generating yellow varieties in horticultural plants.

DOI： 10.1093/pcp/pcz101

「Yuka Takatori」「Keiichi Shimizu」「Jun Ogata」「Hiroki Endo」「Kanji Ishimaru」「Shigehisa Okamoto」「Fumio Hashimoto」 . Cloning of the Flavonoid 3'-Hydroxylase Gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3'H) and Complementation of an F3'H-deficient Mutant of Ipomoea nil (L.) Roth. by Heterologous Expression of EgF3'H . The Horticulture Journal84 ( 2 ) 131 - 139 2015年4月査読

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：The Japanese Society for Horticultural Science (JSHS)

A full-length cDNA of a putative flavonoid 3'-hydroxylase (F3'H) gene encoding a key enzyme in the production of cyanidin was cloned from a lisianthus (Eustoma grandiflorum) petal. Lisianthus F3'H (EgF3'H) shares 75.1, 73.8, and 68.2% amino acid identity with Arabidopsis thaliana, Ipomoea nil, and Petunia hybrida, respectively. RT-PCR revealed that wild-type lisianthus flowers accumulated higher levels of F3'H mRNA during the early stages of development than in the late stages. The accumulated F3'H transcript levels in leaves were similar to those in flowers in the early stages of development. Overexpression of lisianthus F3'H cDNA altered flower color from red to blue in the I. nil cultivar ‘Violet’, which lacks a functional F3'H gene. In addition, the transgenic ‘Violet’ plants accumulated cyanidin and peonidin at similar levels to wild-type I. nil. Taking these findings together, this study demonstrates that EgF3'H functions as a flavonoid 3'-hydroxylase with a role in the synthesis of cyanidin and peonidin pigments.

DOI： http://doi.org/10.2503/hortj.MI-029

髙取　由佳，櫟本　裕太郎，清水　圭一，橋本　文雄 . 野生種トルコギキョウを起源とする二重咲き花形の遺伝様式 . 園芸学研究14 ( 4 ) 341 - 348 2015年査読

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：園芸学会

トルコギキョウの自殖と他殖後代を調査し，一重咲き，二重咲きおよび八重咲きの花形に関する花冠形質の遺伝様式について検討した．八重咲きと一重咲きの交雑後代において明解な分離が認められ，二重咲きの花形は八重咲きと野生種由来または野生種の一重咲きとヘテロ遺伝子型を形成することによってのみ遺伝されることが明らかとなった．野生種を含む固定系統の交雑後代（F1）およびその自殖後代（F2）965個体のデータの調査から，この3種の花冠形質の遺伝は複対立する遺伝子座で制御されると推測された．3種の花形の分離パターンから，トルコギキョウの花における
3つの対立遺伝子D^D，D^SおよびD^Wの存在を説明することが可能である．すなわち，花形の表現型は，D^DD^Dのホモ型およびD^DD^Sのヘテロ型が八重咲き，D^DD^Wのヘテロ型が二重咲き，D^SD^Sのホモ型，D^WD^Wのホモ型およびD^SD^Wのヘテロ型が一重咲きであり，二重咲きに関与する対立遺伝子D^Wは野生種トルコギキョウに起源すると推定した．

DOI： 10.2503/hrj.14.341

Kenichi Shibuya, Keiichi Shimizu, Tomoko Niki, Kazuo Ichimura . Identification of a NAC transcription factor, EPHEMERAL1, that controls petal senescence in Japanese morning glory . The plant jouranal79 ( 6 ) 1044 - 1051 2014年1月査読

Shimizu K., Hashimoto, F. . The Construction of Two New Activation Tagging Vectors, pKANAC2H and pKANAC2K. . Mem. Fac. Agric. Kagoshima Univ.64 37 - 43 2014年1月

Shimizu K., Hashimoto, F. . A New Series of Activation Tagging Vectors, pKANACB, pKANACK and pKANACH. . Mem. Fac. Agric. Kagoshima Univ.63 39 - 47 2013年3月

Keiichi shomizu, Nanako Ohnishi, Noriyuki Morikawa, Ai Ishigami, Saeko Otake,Isselmou Ould Rabah, Yusuke Sakata, Fumio Hashimoto . A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines ofLisianthus [Eustoma grandiflorum (Raf.) Shinn.] . J. Japan. Soc. Hort. Sci. 80 ( 4 ) 434 - 442 2011年4月査読

kenichi Shibuya, keiichi Shimizu, Tetsuya Yamada and Kazuo Ichimura . Expression of autophagy-associated ATG8 genes during petal senescence in Japanese morning glory. . Journal of Japanese Society Horticultural Science.80 89 - 95 2011年1月査読

kenichi Shibuya, Tetsuya Yamada, Tomoko Suzuki, keiichi Shimizu and kazuo Ichimura . InPSR26, a putative membrane protein, regulates programmed cell death during petal senescence in Japanese morning glory. . Plant Physiology. 149 816 - 824 2009年2月査読

Jian-Bin LI, Fumio HASHIMOTO, Keiichi SHIMIZU, Yusuke SAKATA . Anthocyanins from Red Flowers of Camellia saluenensis LINDL. . journal of the japanese society for horticulturel science77 ( 1 ) 75 - 79 2008年1月査読

李家華、石黒悦爾、根角厚司、石川大太郎、清水圭一、坂田祐介、橋本文雄 . 肥料の違いが茶ポリフェノール含量に与える影響 . 農業生産技術管理学会14 ( 2 ) 87 - 92 2007年12月査読

Jian-Bin LI, Fumio HASHIMOTO, Keiichi SHIMIZU, Yusuke SAKATA . Anthocyanins from Red Flowers of Camellia reticulata LINDL. . Bioscience, Biotechnology, and Biochemistry 71 ( 11 ) 2833 - 2836 2007年1月査読

Keiichi Shimizu, Tetsuya Tokumura. Fumio Hashimoto, Yusuke sakata . In Vitro Propagation of Sterile Mutant Strains in Japanese Morning Glory by Sub-culturing Embryoids Derived from an Immature Embryo. . Journal of. Japanese . Society . Horticultural. Science74（4） ( 4 ) 311 - 317 2005年11月査読

keiichi Shimizu, T. Yogai, fumio Hashimoto and Yusuke Sakata . Plant regeneration from suspension culture cell of Delphinium. . Journal of. Japanese . Society . Horticultural. Science73（5） ( 5 ) 435 - 440 2004年9月査読

Fumio Hashimoto, F., A. F. M. J，Uddin, Keiichi Shimizu, Yusuke Sakata . Multiple allelism in Flavonoid hydroxylation in Eustoma grandiflorum (Raf.) Shinn. Flower . Journal of. Japanese . Society . Horticultural. Science73（3） ( 3 ) 235 - 240 2004年5月査読

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