研究者詳細 - 犬童　寛子

論文 - 犬童　寛子

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Cornette R., Indo H.P., Iwata K.i., Hagiwara-Komoda Y., Nakahara Y., Gusev O., Kikawada T., Okuda T., Majima H.J. . Oxidative stress is an essential factor for the induction of anhydrobiosis in the desiccation-tolerant midge, Polypedilum vanderplanki (Diptera, Chironomidae) . Mitochondrion73 84 - 94 2023年11月査読

記述言語：日本語出版者・発行元：Mitochondrion

The sleeping chironomid (Polypedilum vanderplanki) is the only insect capable of surviving complete desiccation in an ametabolic state called anhydrobiosis. Here, we focused on the role of oxidative stress and we observed the production of reactive oxygen species (ROS) in desiccating larvae and in those exposed to salinity stress. Oxidative stress occurs to some extent in desiccating larvae, inducing carbonylation of proteins. Oxidative stress overcomes the antioxidant defenses of the larvae during the first hour following rehydration of anhydrobiotic larvae. It facilitates the oxidation of DNA and cell membrane lipids; however, these damages are quickly repaired after a few hours. In addition to its deleterious effects, we demonstrated that artificial exposure to oxidative stress could induce a response similar to desiccation stress, at the transcriptome and protein levels. Furthermore, the response of anhydrobiosis-related genes to desiccation and salinity stress was inhibited by antioxidant treatment. Thus, we conclude that oxidative stress is an essential trigger for inducing the expression of protective genes during the onset of anhydrobiosis in desiccating of P. vanderplanki larvae.

DOI： 10.1016/j.mito.2023.11.002

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PubMed

Kawashima Y., Miyakoshi M., Kawabata Y., Indo H. . Efficacy of texture analysis of ultrasonographic images in the differentiation of metastatic and non-metastatic cervical lymph nodes in patients with squamous cell carcinoma of the tongue . Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology136 ( 2 ) 247 - 254 2023年8月査読

記述言語：日本語出版者・発行元：Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

Objective: We investigated the efficacy of using texture analysis of ultrasonographic images of the cervical lymph nodes of patients with squamous cell carcinoma of the tongue to differentiate between metastatic and non-metastatic lymph nodes. Study Design: We analyzed 32 metastatic and 28 non-metastatic lymph nodes diagnosed by histopathologic examination on presurgical US images. Using the LIFEx texture analysis program, we extracted 36 texture features from the images and calculated the statistical significance of differences in texture features between metastatic and non-metastatic lymph nodes using the t test. To assess the diagnostic ability of the significantly different texture features to discriminate between metastatic and non-metastatic nodes, we performed receiver operating characteristic curve analysis and calculated the area under the curve. We set the cutoff points that maximized the sensitivity and specificity for each curve according to the Youden J statistic. Results: We found that 20 texture features significantly differed between metastatic and non-metastatic lymph nodes. Among them, only the gray-level run length matrix feature of run length non-uniformity and the gray-level zone length matrix features of gray-level non-uniformity and zone length non-uniformity showed an excellent ability to discriminate between metastatic and non-metastatic lymph nodes as indicated by the area under the curve and the sum of sensitivity and specificity. Conclusions: Analysis of the texture features of run length non-uniformity, gray-level non-uniformity, and zone length non-uniformity values allows for differentiation between metastatic and non-metastatic lymph nodes, with the use of gray-level non-uniformity appearing to be the best means of predicting metastatic lymph nodes.

DOI： 10.1016/j.oooo.2023.04.012

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PubMed

Tomita K., Indo H.P., Sato T., Tangpong J., Majima H.J. . Development of a sensitive double TaqMan Probe-based qPCR Angle-Degree method to detect mutation frequencies . Mitochondrion70 1 - 7 2023年5月査読

記述言語：日本語出版者・発行元：Mitochondrion

We designed a method to examine the mutation frequencies of the A3243G mutation of mitochondrial DNA (mtDNA) in patients with mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome. We performed a qPCR assay using the FAM and VIC TaqMan probes, which detect the 3243G (mutated) and 3243A (wild-type) sequences of mtDNA, respectively. The results obtained by “degree” in a series of differential mutation frequencies were used to plot a standard curve of the mutation frequency. The standard curve was then applied for qPCR assays of the desired samples. The standard deviation (%) of the samples calculated using the standard curve for the TaqMan probe was 2.4 ± 1.5%. This method could be used to examine mutation frequencies in the context of diabetes, aging, cancer, and neurodegenerative diseases.

DOI： 10.1016/j.mito.2023.02.010

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PubMed

Indo H.P., Masuda D., Sriburee S., Ito H., Nakanishi I., Matsumoto K.I., Mankhetkorn S., Chatatikun M., Surinkaew S., Udomwech L., Kawakami F., Ichikawa T., Matsui H., Tangpong J., Majima H.J. . Evidence of Nrf2/Keap1 Signaling Regulation by Mitochodria-Generated Reactive Oxygen Species in RGK1 Cells . Biomolecules13 ( 3 ) 2023年3月査読

記述言語：日本語出版者・発行元：Biomolecules

It has been known that reactive oxygen species (ROS) are generated from the mitochondrial electron transport chain (ETC). Majima et al. proved that mitochondrial ROS (mtROS) caused apoptosis for the first time in 1998 (Majima et al. J Biol Chem, 1998). It is speculated that mtROS can move out of the mitochondria and initiate cellular signals in the nucleus. This paper aims to prove this phenomenon by assessing the change in the amount of manganese superoxide dismutase (MnSOD) by MnSOD transfection. Two cell lines of the same genetic background, of which generation of mtROS are different, i.e., the mtROS are more produced in RGK1, than in that of RGM1, were compared to analyze the cellular signals. The results of immunocytochemistry staining showed increase of Nrf2, Keap1, HO-1 and 2, MnSOD, GCL, GST, NQO1, GATA1, GATA3, GATA4, and GATA5 in RGK1 compared to those in RGM1. Transfection of human MnSOD in RGK1 cells showed a decrease of those signal proteins, suggesting mtROS play a role in cellular signals in nucleus.

DOI： 10.3390/biom13030445

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PubMed

Kawashima Y, Miyakoshi M, Kawabata Y, Indo H. . Normal Cone beam Computed Tomography image findings of nutrient canals in 340 pediatrics. . A J Diagn imaging.9 ( 2 ) 22 - 27 2023年3月査読

Yusuke Kawashima, Masaaki Miyakoshi, Yoshihiro Kawabata, Hiroko. Indo. . Value of MDCT data of mandibular condyle cancellous bone to osteoporosis screening. . A J Diagn imaging.9 ( 1 ) 1 - 7 2023年1月査読

Yusuke Kawashima, Masaaki Miyakoshi, Yoshihiro Kawabata, Hiroko Indo. . The usefulness of intraoral ultrasonography and post-contrast three-dimensional fast field echo imaging in the evaluation of radiological depth of invasion of oral tongue squamous cell carcinoma: Comparison with pathological depth of invasion asessment. . A J Diagn imaging.8 ( 3 ) 61 - 67 2022年12月査読

Indo H., Ito H., Koriyama C., Majima H.J., Shimada K., Tamatsu Y. . Deciduous teeth development in fetuses using postmortem computed tomography image analysis . Legal Medicine54 101969 2022年2月

記述言語：日本語出版者・発行元：Legal Medicine

Fetal age is estimated widely by body length and weight and skeletal or dental development and maturation. Regarding dental development, dental development charts reported by Schour and Massler and Ubelaker are well known. We tried to calculate the calcification rate of the deciduous teeth, mandibular cortical bone, and clavicle in fetuses utilizing postmortem computed tomography (CT) image analysis. The CT values within the circumferential area of the region of interest were automatically calculated using a software, and the calcification rate was calculated by performing single regression analysis. Our results showed that deciduous tooth calcification could be detected in over 19-week-old fetuses using CT images. The calcification of bones (mandibular cortical bone and clavicle) started earlier than the calcification of deciduous teeth. However, the calcification rate of the bones was slower compared to that of the deciduous teeth. The calcification rate of the deciduous teeth in fetuses using CT value may be effective to estimate fetal age and evaluate deciduous teeth development, suggesting that our established method is effective for age estimation in forensic dentistry.

DOI： 10.1016/j.legalmed.2021.101969

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PubMed

川島雄介, 宮腰昌明, 川畑義裕, 阿部浩志, 犬童寛子, 田中達朗 . 両側下顎頭に発生した進行性下顎頭吸収の1例 . 歯科放射線61 ( 2 ) 64 - 68 2022年

川島雄介, 阿部浩志, 宮腰昌明, 川畑義裕, 犬童寛子, 田中達朗 . 高齢者にみられた舌下間隙，顎下間隙および傍咽頭間隙に連続して発生したリンパ管奇形を疑った1例 . 歯科放射線62 ( 1 ) 47 - 50 2022年

川島雄介, 阿部浩志, 宮腰昌明, 川畑義裕, 犬童寛子, 田中達朗 . 頬部に発生した結節性筋膜炎の1例 . 歯科放射線61 ( 2 ) 69 - 71 2022年

川島雄介, 阿部浩志, 宮腰昌明, 川畑義裕, 犬童寛子, 田中達朗 . 舌悪性腫瘍との鑑別を要した魚骨迷入の1例 . 歯科放射線62 ( 1 ) 43 - 46 2022年

Indo H.P., Ito H., Nakagawa K., Chaiswing L., Majima H.J. . Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation . Archives of Biochemistry and Biophysics703 108853 2021年5月

記述言語：日本語出版者・発行元：Archives of Biochemistry and Biophysics

Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.

DOI： 10.1016/j.abb.2021.108853

Scopus

PubMed

Yusuke Kawashima,Masaaki Miyakoshi, Yoshihiro Kawabata, Hiroko Indo. . Usefulness of post-contrast three-dimensional Fast Field Echo imaging compared with Dixon T2-weighted imaging for evaluation of radiological depth of invasion in oral tongue squamous cell carcinoma: Comparison with pathological depth invasion assessment. . A J Diagn imaging. 7 ( 3 ) 13 - 20 2021年1月査読

川島雄介, 原慶宜, 徳永悟士, 犬童寛子, 金田隆 . 左顎下部に発生したRosai-Dorfman diseaseの1例 . 歯科放射線60 ( 2 ) 63 - 65 2021年

Maezawa H, Indo HP, Usami N, Majima HJ, Ito H, Ohnishi K, Kobayashi K. . Enhancement of membrane lipid peroxidation in lung cancer cells irradiated with monoenergetic X-rays at the K-shell resonance absorption peak of phosphorus. . J Radiat Res.61 ( 2 ) 237 - 242 2020年査読

Matsumoto K, Ueno M, Nakanishi I, Indo HP and Majima HJ. . Effects of low-dose X-ray irradiation on melanin-devived radicals in mouse hair and skin. . J Clin Biochem Nutr. 67 ( 2 ) 1 - 5 2020年査読

3) Ito H, Kurokawa H, Suzuki H, Indo HP, Majima HJ, Matsui H. . 5-Aminolevulinic acid induced apoptosis via oxidative stress in normal gastric epithelial cells. . J Clin Biochem Nutr. 65 ( 2 ) 83 - 90 2019年査読

Ken-ichiro Matsumoto, Yukihiro Ogawa, Masato Kamibayashi, Minako Nyui, Ikuo Nakanishi, Shigeaki Suenaga, Hiromu Ito, Hirofumi Matsui, Toshihiko Ozawa, Hiroko P. Indo, Hideyuki J. Majima . Assessment of Redox Status in Commercial Bottled Mineral Water . Journal of Clinical Nutrition and Food Science1 ( 1 ) 29 - 34 2018年10月査読

Ishida T, Hijioka H, Kume K, Yoshimura T, Miyawaki A, Nozoe E, Suenaga S, Indo H, Majima HJ, Nakamura N. . A diagnosis system for detecting cervical lymph node metastasis inoral squamous cell carcinoma: Collective consideration of the results of multiple imaging modalities. . J Oral Maxillofac Surg Med Pathol29 210 - 216 2017年5月査読

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