研究者詳細 - 石橋　松二郎

論文 - 石橋　松二郎

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Fina Amreta Laksmi, Hikari Imamura, Hirohito Tsurumaru, Yoshitaka Nakamura, Hiroshi Hanagata, Shigeki Arai, Masao Tokunaga, Matsujiro Ishibashi . Expression, folding, and activation of halophilic alkaline phosphatase in non-halophilic Brevibacillus choshinensis. . The Protein Journal39 46 - 53 2020年査読

SHOBUDANI MIKU, AUNGZAWHTWE, YAMAKAWA TAKEO, ISHIBASHI MATSUJIRO, TSURUMARU HIROHITO . Mutants Disrupted in the Type Ⅲ Secretion System of Bradyrhizobium elkanii BLY3-8 Overcame Nodulation Restriction by Rj₃-genotype Soybean . Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ35 ( 2 ) 2020年

出版者・発行元：日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

Bradyrhizobium elkanii BLY3-8 does not form nodules on the roots of Rj3-genotype soybean (cultivar D-51). This is a cultivar-specific nodulation restriction. The genes A6X20_40975 and A6X20_41030 in strain BLY3-8 were predicted to encode the transcriptional activator and apparatus of the type III secretion system (T3SS) (the proteins TtsI and RhcJ), respectively. Mutants disrupted in these genes overcame the nodulation restriction. These results suggest that an effector injected via T3SS into Rj3-genotype soybean is involved in nodulation restriction by Rj3-genotype soybean.

DOI： 10.1264/jsme2.ME19151

Scopus

PubMed

CiNii Research

Tokunaga M. . Insoluble expression of highly soluble halophilic metal binding protein for metal ion biosorption: Application of aggregation-prone peptide from hen egg white lysozyme . Protein Expression and Purification156 50 - 57 2019年4月

ARAKAWA Tsutomu, TOKUNAGA Hiroko, ISHIBASHI Matsujiro, TOKUNAGA Masao . Metal Binding Properties of His-Asp Repeat Peptide from Halophilic His-rich Metal Binding Protein Monitored by Circular Dichroism Measurement . 日本海水学会誌73 ( 3 ) 159 - 164 2019年

出版者・発行元：日本海水学会

A His-Asp repeat peptide sequence was identified in a His-rich metal binding protein （HP） produced by a halophilic marine bacterium, Chromohalobacter salexigens DSM 3043T. A synthetic peptide, PepB20, containing 8 His-Asp repeats showed binding to an Ni-loaded metal-chelating column and elution around 150-200 mM imidazole in the presence of both low （0.15 M） and high （1.5 M） concentrations of NaCl. Addition of Ni or Zn ions at 0.1 to 1 mM resulted in changes in secondary structure of PepB20, leading to metal-induced insoluble aggregation at 1 mM. PepB20 showed different circular dichroism （CD） profiles induced by the binding of Ni or Zn ions, suggesting a formation of different secondary structures by these metal ions. We described metal binding properties and consequent structure changes of PepB20 examined by CD measurements and discuss the possible physiological importance of the His-rich metal binding insertion sequence found in HP.

DOI： 10.11457/swsj.73.3_159

CiNii Research

Fina Amreta Laksmi, Shigeki Arai, Hirohito Tsurumaru, Yoshitaka Nakamura,Budi Saksono, Masao Tokunaga, Matsujiro Ishibashi . Improved substrate specificity for D-galactose of L-arabinose isomerase for industrial application . Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics1866 ( 11 ) 1084 - 1091 2018年11月査読

Laksmi F.A. . Improved substrate specificity for D-galactose of L-arabinose isomerase for industrial application . Biochimica et Biophysica Acta - Proteins and Proteomics1866 ( 11 ) 1084 - 1091 2018年11月

Mizukami M. . Efficient production of Trastuzumab Fab antibody fragments in Brevibacillus choshinensis expression system . Protein Expression and Purification150 109 - 118 2018年10月

Ishibashi M. . Expression and characterization of the Renilla luciferase with the cumulative mutation . Protein Expression and Purification145 39 - 44 2018年5月

Matsujiro Ishibashi, Ryo Kawanabe, Norie Amaba, Shigeki Arai, Fina Amreta Laksmi, Kenta Komori, Masao Tokunaga . Expression and characterization of the Renilla luciferase with the cumulative mutation . Protein Expression and Purification145 39 - 44 2018年査読

Megumi Shigehisa, Norie Amaba, Shigeki Arai, Chisato Higashi, Ryo Kawanabe, Ayano Matsunaga, Fina Amreta Laksmi, Masao Tokunaga, Matsujiro Ishibashi . Stabilization of luciferase from Renilla reniformis using random mutations . Protein Engineering, Design and Selection30 ( 1 ) 7 - 13 2017年1月査読

Shigehisa M, Amaba N, Arai S, Higashi C, Kawanabe R, Matsunaga A, Laksmi FA, Tokunaga M, Ishibashi M . Stabilization of luciferase from Renilla reniformis using random mutations. . Protein engineering, design & selection : PEDS30 ( 1 ) 7 - 13 2017年1月

尾辻大樹, 黒木未知瑠, 徳永廣子, 山口類, 徳永雄平, 石橋松二郎, 荒川力, 徳永正雄 . 好塩性ヒスチジン高含有金属結合タンパク質の結合容量と塩環境下での重金属回収の可能性 . 日本海水学会誌71 ( 5 ) 308 - 314 2017年

出版者・発行元：日本海水学会

好塩性海洋細菌Chromohalobacter salexigens由来ヒスチジン高含有金属結合タンパク質（HP）は，Treponema pallidum由来のTroAなどの良く研究されている他の金属結合タンパク質のアミノ酸配列と比較した場合，46アミノ酸残基からなるユニークな挿入配列（多くのヒスチジン残基を含むIS46配列）を持つという特徴がある．この研究では，Zn，Cu，NiもしくはMnを含んだ緩衝液に対してHPの平衡透析を行い，HPの金属結合容量を定量した．その結果，飽和条件下で1分子のHPに最大約10分子のZn，Cu，もしくはNiイオンが結合することが分った．HPに対する金属イオンの親和性は，Zn＞Cu＞Ni＞Mnの順であった．さらに，HPを利用した塩環境下での重金属回収の可能性を試した．His-tagを付与したHP（His-tagged HP）を固定化したSepharoseカラムを作成し，金属結合量を定量したところ1分子のHis-tagged HPあたり約9.5個のNiイオンが結合した．これは塩環境下における重金属の吸着・回収にHPが利用できる可能性を示したものと考えられる．

DOI： 10.11457/swsj.71.5_308

CiNii Research

Shigehisa M. . Stabilization of luciferase from Renilla reniformis using random mutations . Protein Engineering, Design and Selection30 ( 1 ) 7 - 13 2017年

NISHIMOTO Airi, OTSUJI Daiki, TOKUNAGA Hiroko, ISHIBASHI Matsujiro, ARAKAWA Tsutomu, TOKUNAGA Masao . Characterization of Metal Binding Protein ZP and its His-rich Peptides from a Halophilic Marine Bacterium Chromohalobacter salexigens . 日本海水学会誌71 ( 4 ) 252 - 253 2017年

出版者・発行元：日本海水学会

We cloned a gene, Csal-0188, from genomic DNA of Chromohalobacter salexigens DSM 3043T. This gene was annotated to encode Zn-binding protein（ZP）, which showed high homology with the His-rich metal binding protein（HP）we reported. His-tag-ZP was successfully expressed in Escherichia coli and purified. ZP was constructed with a thrombin digestion site so that His-tag could be removed. ZP and chemically synthesized oligo-peptides from the His-rich insertion sequence of ZP showed several metal binding activities. This His-rich peptide was found to bind to Ni column in the presence of 8 M urea, showing clear binding under denaturation conditions, like conventional His-tag.

DOI： 10.11457/swsj.71.4_252

CiNii Research

TOKUNAGA Masao, TOKUNAGA Hiroko, ISHIBASHI Matsujiro, ARAKAWA Tsutomu . Detergent-tolerant Halophilic α-amylase Isolated from a Moderate Halophile Nesterenkonia halobia . 日本海水学会誌71 ( 3 ) 189 - 190 2017年

出版者・発行元：日本海水学会

We examined the effects of several detergents on the stability of halophilic Nesterenkonia halobia α-amylase（NhAmy）. NhAmy was purified to homogeneity from the culture supernatant by simple one-step dextrin affinity column chromatography. The purified enzyme was found to be highly resistant to several detergents, including sodium dodecyl sulfate, which is one of the strongest protein denaturants.

DOI： 10.11457/swsj.71.3_189

CiNii Research

石橋松二郎,大内山愉佳,井上由紀子,岩下彩香,千貫友莉,遠城道雄,徳永正雄 . Dioscorea japonica Thunb.由来DJ1 タンパク質のクローニングと大腸菌による発現と精製 . 応用糖質科学4 ( 3 ) 241 - 248 2014年9月査読

Keita OYAMA, Aya HAYASHI, Takekazu KOBAYASHI, Masao TOKUNAGA and Matsujiro ISHIBASHI . Halo-tolerant Lactic Acid Bacteria from Traditional Salted Pickle Yamagawaduke in Kagoshima. . Bull. Soc. Sea Water Sci., Jpn.68 264 - 265 2014年8月査読

石橋松二郎,大内山諭佳，坂部猛仁，田中玲子，大林朋子，遠城道雄，徳永正雄 . Dioscorea japonica Thunb.で高生産されYam Viscous Polysaccharide (YVP) 画分に含まれるDJ1・タンパク質の精製 . 応用糖質科学4 ( 2 ) 167 - 172 2014年6月査読

Ishibashi M., Arake M., Tokunaga H., Arakawa T., Tokunaga M. . Improved expression in Escherichia coli and stability of halophilic ・B-lactamase-firefly luciferase fusion protein . Bull. Soc. Sea Water Sci.68 341 - 343 2014年査読

Matsujiro Ishibashi, Tomoe Hayashi, Chiho Yoshida, Masao Tokunaga . Increase of salt dependence of halophilic nucleoside diphosphate kinase caused by a single amino acid substitution. . Extremophiles17 ( 4 ) 585 - 591 2013年7月査読

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