2023/10/23 更新

写真a

シライシ ミツヤ
白石 光也
SHIRAISHI Mitsuya
所属
農水産獣医学域獣医学系 共同獣医学部 獣医学科 教授
職名
教授
連絡先
メールアドレス

学位

  • 博士(獣医学) ( 2001年3月   山口大学 )

研究キーワード

  • MARCKSタンパク質

  • 細胞内シグナル伝達

  • 重金属

  • 血小板

  • 血管内皮細胞

  • 神経細胞

研究分野

  • ライフサイエンス / 獣医学

学歴

  • 山口大学   獣医学

    1997年4月 - 2001年3月

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    国名: 日本国

  • 宮崎大学   獣医学科

    1991年4月 - 1997年3月

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    国名: 日本国

経歴

  • 鹿児島大学   農水産獣医学域獣医学系 共同獣医学部 獣医学科 基礎獣医学講座   教授

    2019年2月 - 現在

  • 鹿児島大学   農水産獣医学域獣医学系 共同獣医学部 獣医学科 基礎獣医学講座   准教授

    2012年4月 - 2019年1月

  • 鹿児島大学   農学部獣医学科   准教授

    2007年4月 - 2012年3月

  • 鹿児島大学   農学部獣医学科   助教授

    2007年3月

  • 北里大学   薬学部   助手

    2004年4月 - 2007年2月

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    国名:日本国

  • カルガリー大学   平滑筋研究グループ   研究員

    2001年4月 - 2004年3月

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    国名:カナダ

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所属学協会

  • 日本毒性学会

    2016年5月 - 現在

  • 日本マグネシウム学会

    2008年11月 - 現在

  • 日本薬理学会

    2007年3月 - 現在

  • 日本獣医学会

    2006年12月 - 現在

  • 日本生化学会

    2006年1月 - 現在

  • 日本獣医学会

  • 日本毒性学会

  • 日本マグネシウム学会

  • 日本生化学会

  • 日本薬理学会

▼全件表示

 

論文

  • Wu S., Ootawa T., Sekio R., Smith H., Islam M.Z., Nguyen H.T.T., Uno Y., Shiraishi M., Miyamoto A. .  Reduced Nitric Oxide Synthase Involvement in Aigamo Duck Basilar Arterial Relaxation .  Animals13 ( 17 )   2023年9月査読

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Animals  

    The basilar arterial endothelium mediates blood vessel relaxation partly through the release of nitric oxide (NO). Apoptosis of cerebrovascular endothelial cells is linked to a high mortality rate in chickens infected with the highly pathogenic avian influenza virus, but interestingly, ducks exhibit a greater resistance to this virus. In this study, we examined the responsiveness of duck basilar arteries (BAs) to various vasoactive substances, including 5-hydroxytryptamine (5-HT), histamine (His), angiotensin (Ang) II, noradrenaline (NA), acetylcholine (ACh), and avian bradykinin ornithokinin (OK), aiming to characterize the receptor subtypes involved and the role of endothelial NO in vitro. Our findings suggest that arterial contraction is mediated with 5-HT1 and H1 receptors, while relaxation is induced with β3-adrenergic and M3 receptors. Additionally, OK elicited a biphasic response in duck BAs, and Ang II had no effect. Endothelial NO appears to be crucial in relaxation mediated with M3 and OK receptors but not β3-adrenergic receptors in the duck BA. The reduced endothelial NO involvement in the receptor-mediated relaxation response in duck BAs represents a clear difference from the corresponding response reported in chicken BAs. This physiological difference may explain the differences in lethality between ducks and chickens when vascular endothelial cells are infected with the virus.

    DOI: 10.3390/ani13172740

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  • Wu S., Ootawa T., Sekio R., Smith H., Islam M.Z., Uno Y., Shiraishi M., Miyamoto A. .  Involvement of beta<inf>3</inf>-adrenergic receptors in relaxation mediated by nitric oxide in chicken basilar artery .  Poultry Science102 ( 6 ) 102633 - 102633   2023年6月査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Poultry Science  

    The response of basilar arteries to noradrenaline varies among many animal species, but remains little studied in poultry. Accordingly, we aimed to characterize the adrenergic receptor (AR) subtypes that modulate vascular response in basilar arteries in the chicken, with isometric recording of arterial ring tension using an organ bath. We demonstrated the presence of both alpha and beta (α and β) receptor subtypes through evaluating the response to noradrenaline, with and without a range of β-AR and α-AR antagonists. The concentration-dependent relaxations then induced by a range of β-AR agonists indicated a potency ranking of isoproterenol > noradrenaline > adrenaline > procaterol. We then investigated the effects of β-AR antagonists that attenuate the effect of isoproterenol (propranolol for β1,2,3-ARs, atenolol for β1-ARs, butoxamine for β2-ARs, and SR 59230A for β3-ARs), with Schild regression analysis, ascertaining multiple β-AR subtypes, with neither the β1-AR nor the β2-AR as the dominant subtype. SR 59230A was the only antagonist to yield a pA2 value (7.52) close to the reported equivalent for the relevant receptor subtype. Furthermore, treatment with SR 58611 (a β3-AR agonist) induced relaxation, which was inhibited (P < 0.01) by L-NNA and SR 59230A. Additionally, treating basilar arterial strips (containing endothelium) with SR 58611 induced nitric oxide (NO) production, which was inhibited (P < 0.01) by L-NNA and SR 59230A. Based on this first characterization of AR subtypes in chicken basilar arteries (to our knowledge), we suggest that α- and β-ARs are involved in contraction and relaxation, and that β3-ARs, especially those on the endothelium, may play an important role in vasodilation via NO release.

    DOI: 10.1016/j.psj.2023.102633

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  • Uno Y., Morikuni S., Shiraishi M., Asano A., Murayama N., Yamazaki H. .  Novel Cytochrome P450 2C94 Functionally Metabolizes Diclofenac and Omeprazole in Dogs .  Drug metabolism and disposition: the biological fate of chemicals51 ( 5 ) 637 - 644   2023年5月査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Drug metabolism and disposition: the biological fate of chemicals  

    Cytochromes P450 (P450s or CYPs) are important drug-metabolizing enzymes. Because dogs are frequently used in drug metabolism studies, knowledge of dog CYP2C enzymes is essential because in humans these enzymes are abundant and play major roles in liver and intestine. The present study identified and characterized novel dog CYP2C94 along with previously identified dog CYP2C21 and CYP2C41. Dog CYP2C21, CYP2C41, and CYP2C94 cDNAs, respectively, contained open reading frames of 490, 489, and 496 amino acids and shared high-sequence identities (70%, 75%, and 58%) with human CYP2Cs. Dog CYP2C94 mRNA was preferentially expressed in liver, just as dog CYP2C21 and CYP2C41 mRNAs were. In dog liver, CYP2C21 mRNA was the most abundant, followed by CYP2C94 and CYP2C41 mRNAs. Moreover, the hepatic expressions of all three dog CYP2C mRNAs varied in four individual dogs, two of which did not express CYP2C41 mRNA. The three dog CYP2C genes had similar gene structures, and CYP2C94, although located on the same chromosome, was in a genomic region far from the gene cluster containing CYP2C21 and CYP2C41 Metabolic assays with recombinant proteins showed that dog CYP2C94, along with CYP2C21 and CYP2C41, efficiently catalyzed oxidations of diclofenac, warfarin, and/or omeprazole, indicating that dog CYP2C94 is a functional enzyme. Novel dog CYP2C94 is expressed abundantly in liver and encodes a functional enzyme that metabolizes human CYP2C substrates; it is, therefore, likely responsible for drug clearances in dogs. SIGNIFICANCE STATEMENT: Novel dog cytochrome P450 2C94 (CYP2C94) was identified and characterized along with dog CYP2C21 and CYP2C41. Dog CYP2C94, isolated from liver, had 58% sequence identity and a close phylogenetic relationship with its human homologs and was expressed in liver at the mRNA level. Dog CYP2C94 (and CYP2C21 and CYP2C41) catalyzed oxidations of diclofenac and omeprazole, human CYP2C9 and CYP2C19 substrates, respectively, but CYP2C41 also hydroxylated warfarin. CYP2C94 is therefore a functional drug-metabolizing enzyme likely responsible for drug clearances in dogs.

    DOI: 10.1124/dmd.122.001236

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  • Suzuki M., Wu S., Ootawa T., Smith H., Shiraishi M., Miyamoto A., Matsuoka Y., Sawa S., Mori M., Mori H., Yamori Y. .  Relationship between Regional Distribution of Centenarians and Drinking Water Hardness in the Amami Islands, Kagoshima Prefecture, Japan .  Nutrients15 ( 7 )   2023年3月査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nutrients  

    People who drink naturally hardened water may experience longevity-enhancing effects. In this study, we investigated water hardness and longevity from both geological and epidemiological perspectives in Japan’s Amami islands, where drinking water is drawn from coralline or non-coralline bedrock. We investigated drinking water hardness, limestone bedrock occupancy, and the centenarian rate (number per 10,000 population) by municipality across four adjacent islands (Amami-Oshima (non-coralline), Tokunoshima, Okinoerabu, and Yoron (predominantly coralline)). Limestone was strongly correlated with water hardness (r = 0.99; p < 0.01), occupying more than 80% of the bedrock where the water was the hardest (Tokunoshima’s Isen municipality: 86.5%; Yoron: 82.9%) and being scarcely detectable in Amami-Oshima (0.0 to 0.2%), where the water was the least hard. The centenarian rate was also strongly correlated with water hardness (r = 0.84, p < 0.01), with the highest figures in Yoron (29.7) and Isen (29.2), and the lowest in Amami-Oshima (0.0 to 12.2). Therefore, we hypothesize a potentially beneficial effect of hard water on longevity when that water is drawn from coralline limestone. Water hardness is determined by the water content of calcium and magnesium and may plausibly influence life expectancy through a preventative effect against cardiovascular disease. Our findings are of interest to current debates about future global access to drinking water and its quality.

    DOI: 10.3390/nu15071569

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  • Uno Y, Morikuni S, Shiraishi M, Asano A, Kawaguchi H, Murayama N, Yamazaki H .  A comprehensive analysis of six forms of cytochrome P450 2C (CYP2C) in pigs. .  Xenobiotica; the fate of foreign compounds in biological systems52 ( 9-11 ) 1 - 26   2022年11月査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Xenobiotica  

    Pigs are an important species used in drug metabolism studies; however, the cytochromes P450 (P450s or CYPs) have not been fully investigated in pigs. In this study, pig CYP2C32, CYP2C33, CYP2C34, CYP2C36, CYP2C42, and CYP2C49 cDNAs were isolated and found to contain open reading frames of 490 or 494 amino acids that shared 64–82% sequence identity with human CYP2C8/9/18/19. Pig CYP2C genes formed a gene cluster in a genomic region that corresponded to that of the human CYP2C cluster; an additional gene cluster was formed by pig CYP2C33a and CYP2C33b distant from the first cluster but located in the same chromosome. Among the tissues analysed, these pig CYP2C mRNAs were preferentially expressed in liver, small intestine, and/or kidney; pig CYP2C49, CYP2C32, CYP2C34, and CYP2C33 mRNAs were the most abundant CYP2C mRNAs in liver, jejunum, ileum, and kidney, respectively. Metabolic assays showed that pig CYP2C proteins (heterologously expressed in Escherichia coli) metabolised typical human CYP2C substrates diclofenac, warfarin, and/or omeprazole. The results suggest that these pig CYP2Cs are functional enzymes able to metabolise human CYP2C substrates in liver and small intestine, just as human CYP2Cs do.

    DOI: 10.1080/00498254.2022.2148139

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  • Uno Y., Murayama N., Ijiri M., Kawaguchi H., Yamato O., Shiraishi M., Asano A., Teraoka H., Mizukawa H., Nakayama S.M.M., Ikenaka Y., Ishizuka M., Yamazaki H. .  Cytochrome P450 2J Genes Are Expressed in Dogs, Cats, and Pigs, and Encode Functional Drug-Metabolizing Enzymes .  Drug metabolism and disposition: the biological fate of chemicals50 ( 11 ) 1434 - 1441   2022年11月査読 国際誌

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Drug metabolism and disposition: the biological fate of chemicals  

    Cytochrome P450s (P450s) have been identified and analyzed in dogs and pigs, species that are often used in preclinical drug studies. Moreover, P450s are clinically important for drug therapy not only in humans, but also in species under veterinary care, including dogs and cats. In the present study, seven P450s homologous to human CYP2J2, namely, dog CYP2J2; cat CYP2J2; and pig CYP2J33, CYP2J35, CYP2J91, and CYP2J93, were newly identified and characterized, along with pig CYP2J34 previously identified. The cDNAs of these CYP2Js contain open reading frames of 502 amino acids, except for CYP2J35 (498 amino acids), and share high sequence identity (77%-80%) with human CYP2J2. Phylogenetic analysis revealed that dog and cat CYP2J2 were closely related, whereas pig CYP2Js formed a cluster. All seven CYP2J genes contain nine coding exons and are located in corresponding genomic regions, with the pig CYP2J genes forming a gene cluster. These CYP2J2 mRNAs were predominantly expressed in the small intestine with additional expression in the kidney and brain for dog CYP2J2 and pig CYP2J91 mRNAs, respectively. All seven CYP2Js metabolized human CYP2J2 substrates terfenadine, ebastine, and astemizole, indicating that they are functional enzymes. Dog CYP2J2 and pig CYP2J34 and CYP2J35 efficiently catalyzed ebastine primary hydroxylation and secondary carebastine formation at low substrate concentrations, just as human CYP2J2 does. Velocity-versus-substate plots exhibited sigmoidal relationships for dog CYP2J2, cat CYP2J2, and pig CYP2J33, indicating allosteric interactions. These results suggest that dog, cat, and pig CYP2Js have similar functional characteristics to human CYP2J2, with slight differences in ebastine and astemizole oxidations. SIGNIFICANCE STATEMENT: Dog CYP2J2; cat CYP2J2; and pig CYP2J33, CYP2J34, CYP2J35, CYP2J91, and CYP2J93, homologous to human CYP2J2, were identified and characterized by sequence, phylogenetic, and genomic structure analyses. Intestinal expression patterns of CYP2J mRNAs were characteristic in dogs, cats, and pigs. Dog, cat, and pig CYP2Js likely play roles as drug-metabolizing enzymes in the small intestine, similar to human CYP2J2.

    DOI: 10.1124/dmd.122.000930

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  • Uno Y., Uehara S., Ijiri M., Kawaguchi H., Asano A., Shiraishi M., Banju K., Murayama N., Yamazaki H. .  Molecular and Functional Characterization of N-Acetyltransferases in Common Marmosets and Pigs .  Drug metabolism and disposition: the biological fate of chemicals50 ( 11 ) 1429 - 1433   2022年11月査読 国際誌

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Drug metabolism and disposition: the biological fate of chemicals  

    Arylamine N-acetyltransferases (NATs) are drug-metabolizing enzymes that are essential for the metabolism of endogenous substrates and xenobiotics. The molecular characteristics of NATs have been extensively investigated in humans but remain to be investigated in common marmosets and pigs, animal species that are often used in drug metabolism studies. In this study, marmoset NAT1 and pig NAT1 cDNAs were isolated from liver samples and were characterized by molecular analyses and drug-metabolism assays. These NAT genes were intronless and formed gene clusters with one other NAT gene in the genome, just as human NAT genes do. Marmoset NAT1 and pig NAT1 amino acid sequences showed high sequence identities (94% and 85%, respectively) to human NAT1. Phylogenetic analysis indicated that marmoset NAT1 and pig NAT1 were more closely clustered with human NATs than with rat or mouse NATs. Marmoset NAT1 and pig NAT1 mRNAs were expressed in all the tissue types analyzed, with the expression levels being highest in the small intestine. Metabolic assays using recombinant proteins found that marmoset NAT1 and pig NAT1 metabolized human NAT substrates p-aminobenzoic acid, 2-aminofluorene, sulfamethazine, and isoniazid. Marmoset NAT1 and pig NAT1 substantially acetylated p-aminobenzoic acid and 2-aminofluorene relevant human NAT1, but their activities were lower toward sulfamethazine and isoniazid than those of the relevant human NAT2. Therefore, marmoset and pig NATs are functional enzymes with molecular similarities to human NAT1, but their substrate specificities, while similar to human NAT1, differ somewhat from human NAT2. SIGNIFICANCE STATEMENT: Marmoset N-acetyltransferase NAT1 and pig NAT1 were identified and showed high sequence identities to human NAT1. These NAT mRNAs were expressed in various tissues. Marmoset and pig NAT1s acetylated typical human NAT substrates, although their substrate specificities differed somewhat from human NAT2. Marmoset NAT1 and pig NAT1 have similarities with human NAT1 in terms of molecular and enzymatic characteristics.

    DOI: 10.1124/dmd.122.000919

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  • Nii T., Islam M.Z., Kake S., Shiraishi M., Takeuchi T., Kuwata H., Miyamoto A., Harada E. .  Direct evidence of nitric oxide production induced by lactoferrin and its enhancement by magnesium ions in cultured endothelial cells .  Journal of Veterinary Medical Science84 ( 11 ) 1499 - 1501   2022年9月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本獣医学会  

    Bovine lactoferrin (BLF) reportedly lowers blood pressure and induces vasorelaxation, but its effect on nitric oxide (NO) production has not been established. Accordingly, we aimed to determine whether BLF induces NO production in bovine aortic endothelial cells, and the effects of extracellular free magnesium (Mg) ion concentrations on this NO production. BLF induced NO production time-dependently. NO production was markedly inhibited by the NO synthase inhibitor, NG-nitro-L-arginine methyl ester, in an effect abolished by L-arginine, but not D-arginine. NO production was suppressed at low concentrations, and enhanced at high concentrations, of Mg ions in culture medium. These results suggest that BLF has an important role in hypotensive effects. Mg ions may affect BLF-induced NO production.

    DOI: 10.1292/jvms.22-0368

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  • Naitou K., Iwashita H., Ueda H.H., Shiraishi M., Fujimoto Y., Horii K., Sawamura T., Shiina T., Shimizu Y. .  Intrathecally administered substance P activated the spinal defecation center and enhanced colorectal motility in anesthetized rats .  American Journal of Physiology - Gastrointestinal and Liver Physiology323 ( 1 ) G21-G30 - G30   2022年4月査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Journal of Physiology - Gastrointestinal and Liver Physiology  

    Noxious stimuli on the colorectum cause colorectal contractions through activation of descending monoaminergic pathways projecting from the supraspinal defecation center to the spinal defecation center. Since it is known that substance P is involved in the response to peripheral noxious stimuli in the spinal cord, we investigated the effects of intrathecally administered substance P at L6-S1 levels on colorectal motility in rats that were anesthetized with a-chloralose and ketamine. Intrathecally administered substance P enhanced colorectal motility, even after transection of the thoracic spinal cord at the T4 level. Severing the pelvic nerves, but not the colonic nerves, abolished substance P enhanced colorectal motility. In the spinal cord at L6-S1 levels, expression of mRNA coding neurokinin (NK) 1-3 receptors was detected by RT-PCR. Immunohistological experiments revealed that preganglionic neurons of the pelvic nerves express NK1 receptors, whereas expression of NK2 receptors was not found. In addition, substance P-containing fibers densely innervated around the preganglionic neurons expressing NK1 receptors. An intrathecally administered NK1 receptor antagonist (spantide) attenuated capsaicin-induced colorectal contractions. These results suggest that the colokinetic action of substance P is mediated by the NK1 receptor in the spinal defecation center. Our findings indicate that substance P may function as a neurotransmitter in the spinal defecation center. NEW & NOTEWORTHY We found that intrathecally administered substance P enhanced colorectal motility in anesthetized rats. Neurokinin (NK) 1 receptors, but not NK2 receptors, were detected in preganglionic neurons of the pelvic nerves. Blockade of NK1 receptors in the spinal cord attenuated the enhanced colorectal motility in response to intracolonic noxious stimuli. The findings indicate that substance P may function as a neurotransmitter in the spinal reflex pathway controlling defecation.

    DOI: 10.1152/ajpgi.00342.2021

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  • Ozawa T., Miura N., Hasegawa H., Uemura T., Nakamoto Y., Tsujio M., Takeuchi T., Shiraishi M. .  Characteristics and outcome of suspected cerebrovascular disease in dogs: 66 cases (2009-2016) .  Journal of Small Animal Practice63 ( 1 ) 45 - 51   2022年1月査読

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of Small Animal Practice  

    Objectives : To characterise the clinical signs of suspected cerebrovascular disease in dogs. Materials and Methods : Medical records of one hospital were searched from November 2009 to December 2016 for dogs that suffered of cerebrovascular disease. We diagnosed cerebrovascular disease based on acute onset, clinical signs and magnetic resonance imaging findings. The medical history, clinical signs, concurrent disease, area of infarction, cerebrospinal fluid results, month at onset and outcome were investigated in the cerebrovascular disease group and in a control group (dogs with brain disorders other than cerebrovascular disease). Results : A total of 122 CVD cases were extracted from the 5312 patients that visited during the study period. Of these 122 cases, 66 (1.2%) matched the subject selection criteria of our study and were included in the analysis. Forebrain infarction was observed in 51 of 66 cases, of which 24 (47.1%) suffered from seizures. The number of dogs diagnosed with cerebrovascular disease was disproportionately high in August (nine of 59 cases) and December (13 of 59 cases). In the outcome survey, deterioration was observed in 11 of 55 cases. Clinical Significance : Seizure is an important clinical sign of cerebrovascular disease in dogs. There was a significant seasonal variation in the number of dogs diagnosed with cerebrovascular disease in Japan. Clinical features observed in this report differ from those of previous reports and highlight the need for additional research in this area.

    DOI: 10.1111/jsap.13422

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  • Md Zahorul Islam, Shusuke Kojima, Masamichi Sameshima, Takeshi Obi, Emi Yamazaki-Himeno, Mitsuya Shiraishi, Atsushi Miyamoto .  Vasomotor effects of noradrenaline, 5-hydroxytryptamine, angiotensin II, bradykinin, histamine, and acetylcholine on the bat (Rhinolophus ferrumequinum) basilar artery .  Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology250   109190 - 109190   2021年12月査読 国際共著 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology  

    The responsiveness of the basilar artery to intrinsic vasoactive substances is species-specific and can be a unique characteristic. We investigated the responsiveness of the bat (Rhinolophus ferrumequinum) basilar artery to noradrenaline (NA), 5-hydroxytryptamine (5-HT), angiotensin (Ang) II, bradykinin (BK), histamine (His), and acetylcholine (ACh). NA, 5-HT, Ang II, and BK induced contraction, whereas His and ACh induced relaxation, in a concentration-dependent manner. The NA cumulative concentration–response curve was shifted to the right in parallel with phentolamine (an α-antagonist). However, propranolol, a β-antagonist, had no significant effect. The 5-HT curve was shifted to the right in parallel by ketanserin (a 5-HT2 antagonist) and methiothepin (a 5-HT1 and 5-HT2 antagonist). Losartan (an AT1 antagonist) shifted the Ang II curve to the right, whereas PD123319 (an AT2 antagonist) had no significant effect. L-NA, indomethacin, and des-Arg9-[Leu8]-BK (a B1 antagonist) did not significantly affect BK-induced contractions. HOE140 (a B2 antagonist) shifted the BK concentration–response curve to the right. The His curve was shifted to the right weakly by diphenhydramine (an H1 antagonist) and strongly by cimetidine (a H2 antagonist). ACh-induced relaxation was significantly inhibited by L-NA, atropine, and pFHHSiD (a muscarinic M3 antagonist), whereas pirenzepine and methoctramine (muscarinic M1 and M2 antagonists, respectively) showed no significant effects. At a resting vascular tone, L-NA-induced contraction and indomethacin induced relaxation. These results suggest that α-adrenergic, 5-HT1, 5-HT2, AT1, and B2 receptors might be important in arterial contraction, whereas M3 and H2 (>H1) receptors might modify these contractions, inducing relaxation.

    DOI: 10.1016/j.cbpc.2021.109190

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  • OZAWA Tsuyoshi, IJICHI Takashi, SHIRAISHI Mitsuya .  Measurement of canine blood microparticles by flow cytometry: effect of anticoagulants and staining reagents .  Journal of Veterinary Medical Science83 ( 11 ) 1786 - 1789   2021年11月査読

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本獣医学会  

    <p>Microparticles (MPs) are released from budding plasma membranes into body fluids. The use of flow cytometry for the measurement of MP in canines has not been standardized. In this fundamental study, we compared the effect of anticoagulant agents, such as acid-citrate-dextrose (ACD) and heparin on the measurement of canine MPs in platelet-free plasma (PFP) using flow cytometry. In addition, we used annexin V, carboxyfluorescein succinimidyl ester (CFSE), or calcein tetraacetoxymethyl ester (calcein-AM), and explored the characteristics of the staining reagents in MP detection using flow cytometry. We were able to measure canine MPs in PFP prepared from ACD-anticoagulated blood using flow cytometry, in which the highest positive rate for fluorescent staining was observed when CFSE was used.</p>

    DOI: 10.1292/jvms.21-0448

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  • Ueda H.H., Naitou K., Nakamori H., Horii K., Shiina T., Masatani T., Shiraishi M., Shimizu Y. .  α-MSH-induced activation of spinal MC1R but not MC4R enhances colorectal motility in anaesthetised rats .  Scientific Reports11 ( 1 ) 487 - 487   2021年1月査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientific Reports  

    The central nervous system is involved in regulation of defaecation. It is generally considered that supraspinal regions control the spinal defaecation centre. However, signal transmission from supraspinal regions to the spinal defaecation centre is still unclear. In this study, we investigated the regulatory role of an anorexigenic neuropeptide, α-MSH, in the spinal defaecation centre in rats. Intrathecal administration of α-MSH to the L6-S1 spinal cord enhanced colorectal motility. The prokinetic effect of α-MSH was abolished by severing the pelvic nerves. In contrast, severing the colonic nerves or thoracic cord transection at the T4 level had no impact on the effect of α-MSH. RT-PCR analysis revealed MC1R mRNA and MC4R mRNA expression in the L6-S1 spinal cord. Intrathecally administered MC1R agonists, BMS470539 and SHU9119, mimicked the α-MSH effect, but a MC4R agonist, THIQ, had no effect. These results demonstrate that α-MSH binds to MC1R in the spinal defaecation centre and activates pelvic nerves, leading to enhancement of colorectal motility. This is, to our knowledge, the first report showing the functional role of α-MSH in the spinal cord. In conclusion, our findings suggest that α-MSH is a candidate for a neurotransmitter from supraspinal regions to the spinal defaecation centre.

    DOI: 10.1038/s41598-020-80020-x

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  • Md. Zahorul ISLAM, Yuji SAWATARI, Shusuke KOJIMA, Yusuke KIYAMA, Moe NAKAMURA, Kyouko SASAKI, Mika OTSUKA, Takeshi OBI, Mitsuya SHIRAISHI, Atsushi MIYAMOTO .  Vasomotor effects of 5-hydroxytryptamine, histamine, angiotensin II, acetylcholine, noradrenaline, and bradykinin on the cerebral artery of bottlenose dolphin (Tursiops truncatus) .  Journal of veterinary medical science82 ( 10 ) 1456 - 1463   2020年10月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本獣医学会  

    <p>From an evolutionary aspect, dolphins share a very close phylogenetic relationship with pigs. Previously, we characterized porcine cerebral artery responsiveness to intrinsic vasoactive substances. Therefore, here, we investigated dolphin (<i>Tursiops truncatus</i>) cerebral artery responsiveness to 5-hydroxytryptamine (5-HT), histamine (His), angiotensin (Ang) II, acetylcholine (ACh), noradrenaline (NA), and bradykinin (BK) to characterize their related receptor subtypes. We also compared dolphin cerebral artery responsiveness with porcine cerebral artery responsiveness. We found that 5-HT and His induced concentration-dependent contraction of the dolphin cerebral artery. Ketanserin (a 5-HT<sub>2</sub> antagonist) and methiothepin (a 5-HT<sub>1</sub> and 5-HT<sub>2</sub> antagonist) shifted the concentration-response curve for 5-HT to the right. Although diphenhydramine (an H<sub>1</sub> antagonist) shifted the concentration-response curve for His to the right, cimetidine (an H<sub>2</sub> antagonist) had no such effect. Ang II and ACh did not produce any vasomotor actions. NA induced concentration-dependent relaxation. Propranolol (a β antagonist) shifted the concentration-response curve for NA to the right, whereas phentolamine (an α antagonist) had no significant effect. BK induced relaxation followed by contraction in pre-contracted arteries with intact endothelium. HOE140 (a B<sub>2</sub> antagonist) shifted the concentration-response curve for BK to the right, whereas des-Arg<sup>9</sup>-[Leu<sup>8</sup>]-BK (a B<sub>1</sub> antagonist) had no significant effect. These results suggest that 5-HT<sub>1</sub>, 5-HT<sub>2</sub>, and H<sub>1</sub> receptor subtypes are important in arterial contraction and that β and B<sub>2</sub> receptor subtypes modify these contractions to relaxations. The responsiveness of the dolphin cerebral artery is very similar to that of porcine cerebral artery, supporting their evolutionary linkage.</p>

    DOI: 10.1292/jvms.20-0351

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  • Kasumi SUDO, Cuong VAN DAO, Atsushi MIYAMOTO, Mitsuya SHIRAISHI .  Comparative analysis of in vitro neurotoxicity of methylmercury, mercury, cadmium, and hydrogen peroxide on SH-SY5Y cells. .  Journal of veterinary medical science81 ( 6 ) 828 - 837   2019年6月査読

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:公益社団法人 日本獣医学会  

    <p>Mercury (Hg) and cadmium (Cd) are the major toxic heavy metals and are known to induce neurotoxicity. Although many studies have shown that several heavy metals have neurotoxic effects, the cellular and molecular mechanisms thereof are still not clear. Oxidative stress is reported to be a common and important mechanism in cytotoxicity induced by heavy metals. However, the assays for identifying toxic mechanisms were not performed under the same experimental conditions, making it difficult to compare toxic properties of the heavy metals. In this study, we investigated the mechanisms underlying neurotoxicity induced by heavy metals and H<sub>2</sub>O<sub>2</sub>, focusing on cell death, cell proliferation, and oxidative stress under the same experimental condition. Our results showed that MeHg caused lactate dehydrogenase (LDH) release, caspase activation and cell-cycle alteration, and ROS generation in accordance with decreased cell viability. HgCl<sub>2</sub> caused LDH release and cell-cycle alteration, but not caspase activation. CdCl<sub>2</sub> had a remarkable effect on the cell cycle profiles without induction of LDH release, caspase activation, or ROS generation. Pretreatment with N-acetyl-<span style="font-variant: small-caps;">l</span>-cysteine (NAC) prevented the decrease in cell viability induced by MeHg and HgCl<sub>2</sub>, but not CdCl<sub>2</sub>. Our results demonstrate a clear difference in neurotoxic mechanisms induced by MeHg, HgCl<sub>2</sub>, CdCl<sub>2</sub> or H<sub>2</sub>O<sub>2</sub> in SH-SY5Y cells. Elucidating the characteristics and mechanisms of each heavy metal under the same experimental conditions will be helpful to understand the effect of heavy metals on health and to develop a more effective therapy for heavy metal poisoning.</p>

    DOI: 10.1292/jvms.19-0059

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  • Cuong Van DAO, Mitsuya SHIRAISHI, Atsushi MIYAMOTO .  The MARCKS protein amount is differently regulated by calpain during toxic effects of methylmercury between SH-SY5Y and EA.hy926 cells. .  Journal of Veterinary Medical Science79 ( 12 ) 1931 - 1938   2017年12月査読

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: https://doi.org/10.1292/jvms.17-0473

  • Md.Zahorul Islam, Hiroaki Kawaguchi, Naoki Miura, Noriaki Miyoshi, Emi Yamazaki-Himeno, Mitsuya Shiraishi, Atsushi Miyamoto, AkihideTanimoto .  Hypertension alters the endothelial-dependent biphasic response of bradykinin in isolated Microminipig basilar artery .  Microvascular Research114   52 - 57   2017年11月査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microvascular Research  

    DOI: 10.1016/j.mvr.2017.06.001

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  • Md. Zahorul Islam, Cuong Van Dao, Atsushi Miyamoto, Mitsuya Shiraishi .  Rho-kinase and the nitric oxide pathway modulate basilar arterial reactivity to acetylcholine and angiotensin II in streptozotocin-induced diabetic mice. .  Naunyn-Schmiedeberg's archives of pharmacology390 ( 9 ) 929 - 938   2017年9月査読 国際誌

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Naunyn-Schmiedeberg's Archives of Pharmacology  

    DOI: 10.1007/s00210-017-1396-x

    Web of Science

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  • Cuong Van Dao, Md. Zahorul Islam, Kasumi Sudo, Mitsuya Shiraishi, Atsushi Miyamoto .  MARCKS is involved in methylmercury-induced decrease in cell viability and nitric oxide production in EA.hy926 cells. .  Journal of veterinary medical science78 ( 10 ) 1569 - 1576   2016年11月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1292/jvms.16-0249

  • Ha Thi Thanh Nguyen, Hai Thanh Nguyen, Md. Zahorul Islam, Takeshi Obi, Pitchaya Pothinuch, Thanh Van Nguyen, Tuong Manh Nguyen, Cuong Van Dao, Mitsuya Shiraishi, Atsushi Miyamoto .  Antagonistic effects of Gingko biloba and Sophora japonica on cerebral vasoconstriction in response to histamine, 5-hydroxytryptamine, U46619 and bradykinin .  American journal of chinese medicine44 ( 8 ) 1607 - 1625   2016年11月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1142/S0192415X16500907

  • Masato Ohkubo, Atsushi Miyamoto, Mitsuya Shiraishi .  Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury. .  Journal of veterinary medical science78 ( 5 ) 761 - 767   2016年6月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1292/jvms.15-0620

  • Ha Thi Thanh Nguyen, Hai Thanh Nguyen, Md. Zahorul Islam, Takeshi Obi, Pitchaya Pothinuch, Phyu Phyu Khine Zar, De Xing Hou, Thanh Van Nguyen, Tuong Manh Nguyen, Cuong Van Dao, Mitsuya Shiraishi, Atsushi Miyamoto .  Pharmacological characteristics of Artemisia vulgaris L. in isolated porcine basilar artery. .  Journal of ethnopharmacology182   16 - 26   2016年4月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jep.2016.02.009

  • Md. Zahorul Islam, Cuong Van Dao, Mitsuya Shiraishi, Atsushi Miyamoto .  Methylmercury affects cerebrovascular reactivity to angiotensin II and acetylcholine via Rho-kinase and nitric oxide pathways in mice .  Life sciences147   30 - 38   2016年2月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.lfs.2016.01.033

  • Akihiko Hatano, Mitsuya Shiraishi, Nanae Terado, Atsuhiro Tanabe, Kenji Fukuda .  Enzymatic synthesis and RNA interference of nucleosides incorporating stable isotopes into a base moiety .  Bioorganic & medicinal chemistry23 ( 20 ) 6683 - 6688   2015年10月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bmc.2015.09.011

  • Atsuhiro Tanabe, Mitsuya Shiraishi, Yasuharu Sasaki .  Myristoylated alanine-rich C kinase substrate accelerates TNF-α-induced apoptosis in SH-SY5Y cells in a caspases-6 and/or -7-dependent manner .  Advances in bioscience and biotechnology6 ( 8 ) 572 - 582   2015年8月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.4236/abb.2015.68060

  • Md. Zahorul Islam, Yutaka Watanabe, Ha Thi Thanh Nguyen, Emi Yamazaki-Himeno, Takeshi Obi, Mitsuya Shiraishi, Atsushi Miyamoto .  Vasomotor effects of acetylcholine, bradykinin, noradrenaline, 5-hydroxytryptamine, histamine and angiotensin II on the mouse basilar artery .  Journal of veterinary medical science76 ( 10 ) 1339 - 1345   2014年10月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Md. Zahorul Islam, Kaori Miyagi, Tsukasa Matsumoto, Ha Thi Thanh Nguyen, Emi Yamazaki-Himeno, Mitsuya Shiraishi, Atsushi Miyamoto. .  Bradykinin induces NO and PGF2α production via B2 receptor activation from cultured porcine basilar arterial endothelial cells. .  Naunyn-Schmiedeberg's archives of pharmacology387 ( 7 ) 697 - 702   2014年7月査読

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Mitsuya Shiraishi, Makoto Hangai, Megumi Yamamoto, Masanori Sasaki, Atsuhiro Tanabe, Yasuharu Sasaki, Atsushi Miyamoto .  Alteration in MARCKS phosphorylation and expression by methylmercury in SH-SY5Y cells and rat brain .  Environmental toxicology and pharmacology37 ( 3 ) 1256 - 1263   2014年3月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Atsuhiro Tanabe, Mitsuya Shiraishi, Yasuharu Sasaki .  Dephosphorylation of myristoylated alanine-rich C kinase substrate accelerates wound-induced migration of SH-SY5Y cells .  Advances in bioscience and biotechnology4 ( 8B ) 27 - 32   2013年8月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Mitsuya Shiraishi, Kazuya Tamura, Mina Egoshi, Atsushi Miyamoto .  Cholesterol enrichment of rabbit platelets enhances the Ca(2+) entry pathway induced by platelet-derived secondary feedback agonists .  Life sciences92 ( 14-16 ) 838 - 844   2013年5月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Megumi Yamamoto, Motohiro Takeya, Hiroko Ikeshima-Kataoka, Masato Yasui, Yasuaki Kawasaki, Mitsuya Shiraishi, Eiji Majima, Seiji Shiraishi, Yasuhito Uezono, Masanori Sasaki, Komyo Eto .  Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmoset .  Journal of toxicological sciences37 ( 4 ) 749 - 763   2012年8月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Hideyuki Yamatoya, Hiroaki Kawaguchi, Kanako Yajima, Hidetomi Kadokura, Tsuyoshi Yoshikawa, Rie Yamashita, Mitsuya Shiraishi, Atsushi Miyamoto, Noriaki Miyoshi .  Data on Wistar Hannover rats from a general toxicity study .  Experimental animals61 ( 4 ) 467 - 476   2012年7月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Fukuko Matsumoto, Yutaka Watanabe, Takeshi Obi, M. Z. Islam, Emi Yamazaki-Himeno, Mitsuya Shiraishi, Atsushi Miyamoto. .  Characterization of 5-hydroxytryptamine-induced contraction and acetylcholine-induced relaxation in isolated chicken basilar artery .  Poultry science91 ( 5 ) 1158 - 1164   2012年5月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Hideyuki Yamatoya, Hiroaki Kawaguchi, Takeshi Fukuda, Hidetomi Kadokura, Rie Yamashita, Tsuyoshi Yoshikawa, Mitsuya Shiraishi, Atsushi Miyamoto, Noriaki Miyoshi .  Data on Wistar Hannover rats from an immunotoxicity study .  Experimental animals61 ( 2 ) 171 - 175   2012年4月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Atsuhiro Tanabe, Mitsuya Shiraishi, Manabu Negishi, Naoaki Saito, Mitsuo Tanabe, Yasuharu Sasaki .  MARCKS dephosphorylation is involved in bradykinin-induced neurite outgrowth in neuroblastoma SH-SY5Y cells .  Journal of cellular physiology227   618 - 629   2012年2月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Miki Nakashima, Yukiko Shigekuni, Takeshi Obi, Mitsuya Shiraishi, Atsushi Miyamoto, Hideo Yamasaki, Takeomi Etoh, Sumio Iwai. .  Nitric oxide-dependent hypotensive effects of wax gourd juice .  Journal of ethnopharmacology138 ( 2 ) 404 - 407   2011年11月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Mitsuya Shiraishi, Eisuke Tani, Atsushi Miyamoto .  Modulation of rabbit platelet aggregation and calcium mobilization by platelet cholesterol content .  Journal of veterinary medical science72 ( 3 ) 285 - 292   2010年3月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Hiroki Yamaguchi, Mitsuya Shiraishi, Kiyoko Fukami, Atsuhiro Tanabe, Yuri Ikeda-Matsuo, Yasuhito Naito, Yasuharu Sasaki .  MARCKS regulates lamellipodia formation induced by IGF-I via association with PIP2 and beta-actin at membrane microdomains .  Journal of cellular physiology220 ( 3 ) 748 - 755   2009年9月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Daisaku Ueno, Akira Yabuki, Takeshi Obi, Mitsuya Shiraishi, Akira Nishio, Atsushi Miyamoto .  Characterization of bradykinin-induced endothelium-independent contraction in equine basilar artery .  Journal of cellular physiology32 ( 3 ) 264 - 270   2009年6月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Kosuke Takeya, Kathy Loutzenhiser, Mitsuya Shiraishi, Rodger Loutzenhiser, Michael P. Walsh .  A highly sensitive technique to measure myosin regulatory light chain phosphorylation: the first quantification in renal arterioles .  American journal of physiology. Renal physiology294 ( 6 ) F1487 - F1492   2008年6月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Tadatsune Okuno, Akira Yabuki, Mitsuya Shiraishi, Takeshi Obi, Atsushi Miyamoto .  Histamine-induced modulation of vascular tone in the isolated chicken basilar artery: A possible involvement of endothelium .  Comparative biochemistry and physiology - part C: toxicology & pharmacology 147 ( 3 ) 339 - 344   2008年4月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Narihiro Yoshinaga,Tadatune Okuno, Yutaka Watanabe, Tsukasa Matsumoto, Mitsuya Shiraishi, Takeshi Obi, Akira Yabuki, Atsushi Miyamoto .  Vasomotor effects of noradrenaline, acetylcholine, histamine, 5-hydroxytryptamine and bradykinin on snake (Trimeresurus flavoviridis) basilar arteries .  Comparative biochemistry and physiology - part C: toxicology & pharmacology 146 ( 4 ) 478 - 483   2007年11月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Mitsuya Shiraishi, Atsuhiro Tanabe, Naoaki Saito, Yasuharu Sasaki .  Unphosphorylated MARCKS is involved in neurite initiation induced by insulin-like growth factor-I in SH-SY5Y cells .  Journal of cellular physiology209 ( 3 ) 1029 - 1038   2006年12月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Ruriko Koto, Masatoshi Imamura, Chie Watanabe, Satoshi Obayashi, Mitsuya Shiraishi, Yasuharu Sasaki, Hiroshi Azuma .  Linalyl acetate as a major ingredient of lavender essential oil relaxes the rabbit vascular smooth muscle through dephosphorylation of myosin light chain .  Journal of cardiovascular pharmacology48 ( 1 ) 850 - 856   2006年7月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Greg A. Knock, Anushika S. De Silva, Vladimir A. Snetkov, Richard Siow, Gavin D. Thomas, Mitsuya Shiraishi, Michael P. Walsh, Jeremy P. T. Ward, Philip I. Aaronson .  Modulation of PGF(2 alpha)- and hypoxia-induced contraction of rat intrapulmonary artery by p38 MAPK inhibition: a nitric oxide-dependent mechanism .  American journal of physiology. Lung cellular and molecular physiology 289 ( 6 ) L1039 - L1048   2005年12月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Mitsuya Shiraishi, Rodger D. Loutzenhiser, Michael P. Walsh .  A highly sensitive method for quantification of myosin light chain phosphorylation by capillary isoelectric focusing with laser-induced fluorescence detection .  Electrophoresis26 ( 3 ) 571 - 580   2005年2月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Erika Shimomura, Mitsuya Shiraishi, Takahiro Iwanaga, Minoru Seto, Yasuharu Sasaki, Masahiro Ikeda, Katsuaki Ito .  Inhibition of protein kinase C-mediated contraction by Rho kinase inhibitor fasudil in rabbit aorta .  Naunyn-Schmiedeberg's archives of pharmacology370 ( 5 ) 414 - 422   2004年11月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Mitsuya Shiraishi, Xuemei Wang, Michael P. Walsh, Gary Kargacin, Kathy Loutzenhiser, Rodger Loutzenhiser .  Myosin heavy chain expression in renal afferent and efferent arterioles: relationship to contractile kinetics and function .  FASEB journal17 ( 13 ) 2284 - 2286   2003年10月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Katsuaki Ito, Erika Shimomura, Takahiro Iwanaga, Mitsuya Shiraishi, Kazutoshi Shindo, Junji Nakamura, Hiromitsu Nagumo, Minoru Seto, Yasuharu Sasaki, Yoh Takuwa .  Essential role of rho kinase in the Ca2+ sensitization of prostaglandin F-2 alpha-induced contraction of rabbit aortae .  Journal of physiology (London)546 ( 3 ) 823 - 836   2003年2月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Mitsuya Shiraishi, Sachiko Kawashima, Masaaki Moroi, Yangchol Shin, Takashi Morita, Yoichiro Horii, Masahiro Ikeda, Katsuaki Ito .  A defect in collagen receptor-Ca2+ signaling system in platelets from cattle with Chediak-Higashi syndrome .  Thrombosis and haemostasis87 ( 2 ) 334 - 341   2002年2月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Mitsuya Shiraishi, Masahiro Ikeda, Takeshi Fujishiro, Kiichi Fukuyama, Katsuaki Ito .  Characteristics of collagen-induced Ca2+ mobilization in bovine platelets .  Cell calcium27 ( 1 ) 53 - 60   2000年1月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Mitsuya Shiraishi, Masahiro Ikeda, Hiroyuki Ogawa, Chien-HsienTu, Katsuaki Ito .  Impaired cytosolic calcium mobilization and aggregation in response to collagen in platelets from Japanese Black cattle with Chediak-Higashi syndrome .  American journal of veterinary research59 ( 6 ) 744 - 749   1998年6月査読

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

▼全件表示

MISC

  • 【マグネシウム代謝-その新たな臨床的意義】基礎と総論 マグネシウム濃度と生命予後との関連性 血圧と飲料水を中心に

    鈴木 舞, 新居 朋昭, 松岡 由紀, 澤 佐和子, 白石 光也, 宮本 篤

    腎と透析   86 ( 2 )   163 - 166   2019年2月

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    記述言語:日本語   出版者・発行元:(株)東京医学社  

  • Platelet dysfunction in Chediak-Higashi syndrome-affected cattle 査読

    Mitsuya Shiraishi, Hiroyuki Ogawa, Masahiro Ikeda, Sachiko Kawashima, Katsuaki Ito

    Journal of veterinary medical science   64 ( 9 )   751 - 760   2002年4月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

講演・口頭発表等

  • 呉思遠、大田和朋紀、宇野泰弘、白石光也、宮本篤 .  ニワトリ脳底動脈のβ受容体を介した弛緩反応に関与する受容体サブタイプとその特性 .  第165回日本獣医学会学術集会  2022年9月 

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    開催年月日: 2022年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

  • 辻尾祐志、白石光也、松元光春 .  コロナ禍における鹿児島大学の獣医解剖・組織学教育の実施方法と今後の取り組み .  第165回日本獣医学会学術集会  2022年9月  招待

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    開催年月日: 2022年9月

    記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(指名)  

  • 大田和朋紀、呉思遠、白石光也、宇野泰広、宮本篤 .  近接する2島である奄美大島と徳之島に生息する同種のハブの胸大動脈でみられた血管反応性の違い .  第165回日本獣医学会学術集会  2022年9月 

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    開催年月日: 2022年9月

    記述言語:日本語   会議種別:口頭発表(一般)  

  • Kiyotada Naitou, Honoka Iwashita, Hiromi H. Ueda, Mitsuya Shiraishi, Yoshikazu Fujimoto, Kazuhiro Horii, Tomoya Sawamura, Takahiko Shiina and Yasutake Shimizu .  Spinal Substance P Related To Defecation Reflexes And Enhanced Colorectal Motility. .  International Society for Autonomic Neuroscience 2022  2022年9月  nternational Society of Autonomic Neuroscience国際会議

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    開催年月日: 2022年9月

    記述言語:英語   会議種別:ポスター発表  

    開催地:Cairns Convention Centre   国名:オーストラリア連邦  

  • 植田大海、内藤清惟、白石光也、児島一州、正谷達謄、 椎名貴彦、志水泰武 .  脊髄排便中枢においてα-MSH は 1 型メラノコルチン受容体 (MC1R)を介して大腸運動を促進する .  第31回日本病態生理学会大会  2022年8月 

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    開催年月日: 2022年8月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岐阜大学 柳戸キャンパス  

    その他リンク: http://31st-pathophysiology.kenkyuukai.jp/images/sys/information/20220720133221-0D6DDB2185BE343443736EA0F05E67C1AB13F8CEDA321C0D982C01327B7FD039.pdf

  • Siyuan Wu, Ryoya Sekio, Henry Smith, Mitsuya Shiraishi, Atsushi Miyamoto .  Noradrenaline-induced basilar artery vasomotor response also varies in avian species (chickens and ducks) .  第95回日本薬理学会年会  2022年3月 

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    開催年月日: 2022年3月

    記述言語:英語   会議種別:口頭発表(一般)  

    その他リンク: https://pharmacology.main.jp/jps95/download/pdf/oral_0309.pdf

  • 宮本篤、呉思遠、大田和朋紀、関尾椋哉、宇野泰弘、白石光也、Md. Zahorul Islam .  摘出脳底動脈におけるヒスタミン血管反応性の動物種差 .  第23回日本ヒスタミン学会  2022年1月 

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    開催年月日: 2022年1月

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:京都大学医学部 芝蘭会館別館  

  • 鈴木 舞, 白石 光也, 松岡 由紀, 澤 佐和子, 森 真理, 森 英樹, 家森 幸男, 宮本 篤 .  徳之島伊仙町の飲料水を用いて調製したクレブスリンゲル液の脳血管反応性に及ぼす影響 .  第37回日本マグネシウム学会  2017年11月  (一社)日本マグネシウム学会

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    開催年月日: 2017年11月

    記述言語:日本語   会議種別:口頭発表(一般)  

  • 内藤 清惟, 岩下 朋乃樺, 植田 大海, 白石 光也, 藤本 佳万, 椎名 貴彦, 志水 泰武 .  脊髄排便中枢におけるサブスタンスPの大腸運動促進作用 .  日本獣医学会学術集会講演要旨集  2021年9月  (公社)日本獣医学会

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    記述言語:日本語  

  • 植田 大海, 内藤 清惟, 白石 光也, 児島 一州, 正谷 達謄, 椎名 貴彦, 志水 泰武 .  脊髄排便中枢においてα-MSHは1型メラノコルチン受容体(MC1R)を介して大腸運動を促進する .  日本病態生理学会雑誌  2022年7月  日本病態生理学会

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    記述言語:日本語  

  • 牧野 遥, 正谷 達謄, 藤本 佳万, 内藤 清惟, 白石 光也 .  未分化型および分化型ヒト由来培養神経細胞におけるウイルス感受性の比較 .  日本獣医学会学術集会講演要旨集  2021年9月  (公社)日本獣医学会

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    記述言語:日本語  

  • 呉 思遠, 大田和 朋紀, 宇野 泰広, 白石 光也, 宮本 篤 .  ニワトリ脳底動脈のβ受容体を介した弛緩反応に関与する受容体サブタイプとその特性 .  日本獣医学会学術集会講演要旨集  2022年9月  (公社)日本獣医学会

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    記述言語:日本語  

  • 辻尾 祐志, 白石 光也, 松元 光春 .  コロナ禍及びこれからの獣医解剖学実習 コロナ禍における鹿児島大学の獣医解剖・組織学教育の実施方法と今後の取り組み .  日本獣医学会学術集会講演要旨集  2022年9月  (公社)日本獣医学会

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    記述言語:日本語  

▼全件表示

共同研究・競争的資金等の研究

  • 有害金属の発達神経毒性におけるMARCKSタンパク質の関与とその役割

    研究課題/領域番号:19K06404  2019年4月 - 2022年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    白石 光也

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    配分額:3770000円 ( 直接経費:2900000円 、 間接経費:870000円 )

    重金属による健康被害は現在でも大きな課題の一つであり、一部の重金属は特に脳や神経細胞の発育期に毒性を示すことが知られている。そこで、本研究では分化誘導刺激を行った神経細胞株を発達神経細胞モデルとして、メチル水銀暴露による毒性作用およびMARCKSタンパク質への関与を明らかにすることを目的とした。実験には、ヒト神経芽細胞腫由来の培養神経細胞株であるSH-SY5Y細胞を使用した。分化過程にある神経細胞へのメチル水銀毒性を評価するため、神経の分化を誘導するレチノイン酸を培養神経細胞に処置し、発達神経細胞モデルとして使用した。レチノイン酸およびメチル水銀によるSH-SY5Y細胞への影響を、CCK8による細胞生存率測定およびフローサイトメトリ―による細胞周期解析により検討した。また、MARCKSの発現量およびリン酸化への影響は、Western Blot 法により解析した。培地中のFBS 濃度を低濃度(1%)に調節することで、SH-SY5Y細胞へのメチル水銀処置による影響を長期間(6 日間)観察することが可能となった。レチノイン酸を処置した発達神経細胞モデルでは、未処置群と比べメチル水銀による細胞生存率の低下が増強されていた。この結果は、メチル水銀が分化過程にある神経細胞に、より強く毒性を示す特徴を反映したものと考えられた。一方、レチノイン酸の処置は細胞周期に影響しなかったことから、レチノイン酸によるメチル水銀毒性の増強に細胞周期は関与しないと考えられた。また、SH-SY5Y細胞へのレチノイン酸処置によりMARCKS発現量の減少、およびリン酸化MARCKS量の顕著な上昇が認められたことから、このMARCKSの変化がメチル水銀に対する感受性の増加に関与している可能性が示唆された。

  • 血小板,血管平滑筋および内皮細胞におけるMARCKSの役割と病的血栓形成への関与

    研究課題/領域番号:26450407  2014年4月 - 2018年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    白石 光也, 宮本 篤

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    配分額:4940000円 ( 直接経費:3800000円 、 間接経費:1140000円 )

    血管内皮細胞、平滑筋細胞および血小板における、未だその詳細が明らかとなっていないMARCKSタンパク質の役割を検討した。血管内皮細胞において、MARCKSタンパク質は細胞増殖、細胞運動、一酸化窒素産生などその重要な機能に関わることを明らかにした。また、糖尿病およびメチル水銀中毒モデル動物における異常にも関与している可能性が示された。また、MARKCSタンパク質は平滑筋細胞および血小板にも発現しており、これらの細胞における役割が考えられた。