Language：Japanese   Publisher：Annals of Anatomy  

Background: Alpha-synuclein (Syn), an unfolded soluble cytosolic protein, is known as a disease-associated protein in the brain. However, little is known about distribution of this protein in the peripheral nervous system. In this study, expression of Syn was investigated in the sensory ganglia of the cranial nerves V, IX and X. Methods: To analyze distribution of Syn and its co-expression with calcitonin gene-related peptide (CGRP) or the transient receptor potential cation channel subfamily V member 1 (TRPV1), immunohistochemical techniques were used in the rat cranial sensory ganglia and their peripheral tissues. Results: Syn-immunoreactive (-ir) neurons were abundant in the sensory ganglia of the petrosal (56.7%), jugular (28.3%) and nodose ganglia (82.5%). These neurons had small to medium-sized cell bodies (petrosal, mean ± S.D. = 667.4 ± 310.8 μ m2; jugular, 625.1 ± 318.4 μ m2; nodose, 708.3 ± 248.3 μ m2), and were distributed throughout the ganglia. However, the trigeminal ganglion was mostly free of Syn-ir neurons. By double and triple immunofluorescence staining, Syn-ir neurons co-expressed CGRP and TRPV1 in the petrosal and jugular ganglia. Syn-immunoreactivity was expressed by nerve fibers in the epithelium and taste bud of oral and cervical viscerae. These nerve fibers were abundant in the naso-pharynx, epiglottis and laryngeal vestibule. Some taste bud cells were also immunoreactive for Syn. In addition, Syn-ir nerve fibers were detected in the vicinity of macrophages, dendritic cells and Langerhans cells. Conclusions: Syn was abundant in the visceral sensory neurons but not in somatic sensory neurons. This protein may play a role in nociceptive and chemosensory transduction in the glossopharyngeal and vagal sensory ganglia. It is possible that Syn has a function about the immune mechanism of the upper air way.

DOI： 10.1016/j.aanat.2021.151776

Scopus

PubMed

Language：Japanese   Publisher：Japanese Association of Regenerative Dentistry  

<p>We prepared experimental pulp capping materials those were consist of Mineral Trioxide Aggregate (MTA) and a hydrophilic monomer in order to develop an adhesive pulp capping material. The purpose of this study was to investigate dentin shear bond strengths and releasing of calcium ions of those experimental or commercial pulp capping materials.</p><p>As a result, two experimental pulp capping materials had excellent marginal sealing property than commercial pulp capping agents, even higher shear bond strengths.　In a comparison between experimental pulp capping agents having different composition ratios of monomer and MTA, the group with a relatively large amount of monomer showed higher shear bond strengths. The group with a large amount of MTA showed no penetration of dye in the dye penetration canal and releasing amount of calcium ion was higher than the group with a small amount of MTA. The composition ratio of MTA and a hydrophilic monomer may affect the basic property of the pulp capping material.</p><p>More research is required to determine the ideal composition ratio of MTA/monomer to develop an adhesive pulp capping material that has excellent dentin marginal sealing property and exhibits highest adhesive strength.</p>

DOI： 10.11223/jard.19.1

Language：Japanese   Publisher：日本再生歯科医学会  

接着性を有する覆髄剤の開発を目的として、Mineral trioxide aggregate(MTA)と親水性モノマーで重合硬化させる覆髄剤を試作した。MTAとモノマーの比率が異なる2種の試作覆髄剤および市販の覆髄剤を実験に供した。材料の基本性能となる象牙質と試作覆髄剤のせん断接着強さ、象牙質辺縁封鎖性および試作覆髄剤からのカルシウムイオン徐放について検討を行った。その結果、本研究で試作した覆髄剤は、市販の覆髄剤よりも良好な象牙質辺縁封鎖性を示し、接着強さも高かった。またモノマーとMTAの配合比が異なる試作覆髄剤間での比較では、相対的にモノマーの比率が高い方が接着強さは高く、MTAの比率が高い覆髄剤では象牙質辺縁封鎖性に優れ、材料からのカルシウムイオン徐放量が多くなる可能性が示唆された。MTAとモノマーの配合比率が覆髄剤の基本性能に影響する可能性があることから、今後さらに、象牙質辺縁封鎖性に優れた高い接着強さが得られる配合比率について検討を行う予定である。(著者抄録)

Language：Japanese   Publishing type：Research paper (scientific journal)  

Publisher：日本再生歯科医学会  

コロイド状白金ナノ粒子(CPN)が象牙質接着強さに及ぼす影響を検討した。抜歯したヒト臼歯21本を対照群と2種類の実験群に分け、接着剤にはCLEARFILユニバーサルボンド(CUB)を用い、表面処理剤にはK ETCHANT syringe(ECH)とCPNを用いた。対照群はCUBのみで処理し、実験群はCUB+Ech(ECH群)またはCUB+CPN(CPN群)で処理して、樹脂複合材の形成後に37℃水中に24時間保管した後、接着面が1mm2の試料を作成して微小引張り強さ(μTBS)を試料調製の直後と6ヵ月後に測定した。その結果、24時間後の接着強さは対照群と実験群の間で差が見られなかったが、6ヵ月後に接着強さの有意な低下が見られなかったのはCPN群のみであった。以上より、CUBとCPNを併用すると長期の接着信頼性が得られると考えられた。

Event date： 2019.11

Language：English   Presentation type：Oral presentation (general)  

Event date： 2019.11

Language：Japanese   Presentation type：Poster presentation  

Event date： 2019.10

Language：English   Presentation type：Oral presentation (general)  

Event date： 2019.6

Language：Japanese   Presentation type：Poster presentation  

Event date： 2018.11

Language：Japanese   Presentation type：Oral presentation (general)