研究者詳細 - 松尾　智英

Ijiri M., Akioka K., Kitano T., Miura H., Kono H., Terashima R., Fujimoto Y., Matsuo T., Yamato O., Kawaguchi H. . Acupuncture Treatment Improves Transport Stress in Microminipigs Through the Acupoint in Ears . In Vivo37 ( 5 ) 2100 - 2104 2023年9月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：In Vivo

Background/Aim: This study aimed to investigate the effects of acupuncture treatment through the ear acupoints on transport stress in experimental microminipigs. Materials and Methods: Experiment 1: Six animals were equally divided into two groups (Control and Treatment). In the treatment group, before transportation (6 h; vehicle and plane), short, ultrathin circular transdermal needles were applied to locations corresponding to the acupoints on the apical area of both ears. Peripheral blood samples were collected from the cranial vena cava 2 days before and immediately after transportation. Blood stress markers, biochemistry indicators, and oxidative stress levels were examined. Experiment 2 (follow-up study: diarrhea incidence after transportation): Diarrhea incidence after transportation in the control and treatment groups was investigated. Results: Experiment 1: Transport stress induced an increase in blood cortisol, serum amyloid A (SAA), glucose, non-esterified fatty acid, and derivatives of reactive oxygen metabolites (d-ROMs) and decreased the biological antioxidant potential (BAP)/d-ROMs ratio yet did not affect BAP. Acupuncture suppressed the increases in SAA and d-ROMs values and the decrease in BAP/d-ROMs ratio. Experiment 2: The total diarrhea incidence was 25% in the control group, whereas diarrhea was not observed in the treatment group. Conclusion: Acupuncture treatment suppresses hypothalamic–pituitary–adrenal function and, as a result, reduces transport stress without affecting the suppression of the central catecholaminergic system. Acupuncture treatment for transport stress can improve animal welfare.

DOI： 10.21873/invivo.13307

高橋和瑛, 松尾智英, 山岸潤也, 芝原友幸, 笹井和美, 松林誠 . 一酪農家におけるクリプトスポリジウム症の清浄化への試み . 家畜診療70 ( 7 ) 407 - 416 2023年7月

Fujimoto Y., Ijiri M., Matsuo T., Kawaguchi H. . In vitro antiviral activity of persimmon-derived tannin against avian influenza viruses . Letters in Applied Microbiology76 ( 2 ) 2023年2月国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Letters in Applied Microbiology

Tannins derived from natural plant sources are known to provide many health benefits to humans and animals. Among the various tannins, those derived from persimmon (Diospyros kaki) have exhibited strong inactivating effects against pathogens that induce diseases in humans. However, few studies have focused on the antiviral effects of persimmon tannin against pathogen-induced diseases in animals. In this study, we investigated the antiviral effects of persimmon tannin against various avian influenza viruses revealing that tannin at a concentration of 1.0 mg ml-1 reduced viral infectivity in >6.0-log scale against all tested avian influenza viruses. In addition, this persimmon tannin concentration effectively inhibited the receptor binding and membrane fusion abilities of viral hemagglutinin (HA), which play important roles in avian influenza virus infection. These results suggest that persimmon tannin inactivates the HA of avian influenza viruses and reduces their infectivity. Persimmon tannin is a safer natural substance than the currently used chemical compound related to antiviral substance. When inactivation of the viruses which are present in environmental water such as roosting water of wild birds will be needed, persimmon tannin is expected to become an antiviral resource that may prevent the spread of several avian influenza virus subtypes.

DOI： 10.1093/lambio/ovac053

高橋和瑛, 松尾智英, 山岸潤也, 芝原友幸, 笹井和美, 松林誠 . 一酪農場におけるクリプトスポリジウム症の清浄化への試み . 家畜診療70 ( 1 ) 47 - 48 2023年1月

Ijiri M., Yoshinaga K., Kawaguchi H., Miyazaki N., Kawaguchi M., Fujimoto Y., Matsuo T., Maruyama I. . In Vivo Metabolism of 1,5-Anhydro-d-fructose to 1,5-Anhydro-d-glucitol . In Vivo37 ( 3 ) 1022 - 1027 2023年国際誌

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：In Vivo

Background/Aim: 1,5-Anhydro-D-fructose (1,5-AF, saccharide) and 1,5-anhydro-D-glucitol (1,5-AG) converted from 1,5-AF via the glycemic pathway have health benefits. However, this metabolism has not been sufficiently elucidated. To clarify the in vivo metabolism of 1,5-AF to 1,5-AG, porcine (blood kinetics) and human (urinary excretion) studies were conducted. Materials and Methods: Microminipigs were administrated 1,5-AF orally or intravenously. Blood samples were obtained to analyse the kinetics of 1,5-AF and 1,5-AG. Urine samples were collected from human subjects who had orally ingested 1,5-AF, and the amounts of 1,5-AF and 1,5-AG excreted in the urine were analysed. Results: In blood kinetics analysis, the time to the maximum concentration of 1,5-AF after intravenous administration was 0.5 h, whereas 1,5-AF was not observed after oral administration. The times to the maximum concentration of 1,5-AG after intravenous and oral administration were 1.5 h and 2 h, respectively. In urinary excretion, the concentration of 1,5-AG in urine rapidly increased after the administration of 1,5-AF, peaked at 2 h, whereas 1,5-AF was not detected. Conclusion: 1,5-AF was rapidly metabolized to 1.5-AG in vivo in swine and human.

DOI： 10.21873/invivo.13176

Ijiri M., Odo K., Sato M., Kawaguchi M., Fujimoto Y., Miura N., Matsuo T., Hou D.X., Yamato O., Tanabe T., Kawaguchi H. . Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region . Journal of Veterinary Research (Poland)66 ( 2 ) 209 - 214 2022年6月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Veterinary Research (Poland)

Introduction: Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons. Material and Methods: Pigs were allocated to summer- and winter-finishing cohorts, 12 each. The evaluations included assessment of carcass traits and internal organs’ normality carried out at the time of slaughter, and measurement of biomarkers in whole blood: derivatives of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential as markers of oxidative stress, and serum amyloid A and albumin/globulin (A/G) ratio as markers of acute and chronic inflammation, respectively. Results: The summer-finished pigs reared under subtropical field conditions showed lower carcass quality than the winter-finished pigs, indicating a potential adverse effect of summer temperatures on the swine industry. Marginal changes were observed in d-ROMs and the A/G ratio between the summer- and winter-finishing cohorts. Conclusion: The results demonstrate that d-ROMs and the A/G ratio could be used as sensitive markers for heat stress under field conditions.

DOI： 10.2478/jvetres-2022-0024