Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Frontiers in Education  

Introduction: The Mishima Islands in Kagoshima Prefecture, Japan, are remote islands designated as medically underserved. Supporting the people living in such remote islands requires a multifaceted examination of the livelihoods of the residents, including their health, work, and living environment. Methods: Students from the faculties of nursing, veterinary science, agriculture, and engineering participated in a 3-day virtual fieldwork session held synchronously from the campus of Kagoshima University and on a remote island in the Mishima Mura island group in Kagoshima Prefecture, Japan. Coursework and post-course surveys were analyzed to determine student engagement and learning. In particular, the analysis considered learning in relation to interprofessional learning and community-based learning. Results and discussion: Students expressed being able to conduct fieldwork online, collaborate with students from multiple faculties and graduate schools, and apply their expertise. These outcomes were reflected in the five-point Likert scale evaluation responses, highlighting a sense of accomplishment and satisfaction. Virtual fieldwork can contribute to student learning by introducing new concepts and allowing them to consider development issues in remote areas from multiple perspectives.

DOI： 10.3389/feduc.2024.1421218

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DOI： 10.1111/vco.12954

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Veterinary Anesthesia and Surgery  

<p>Malignant tumors arising from thoracic wall may require extensive resection, including ribs. The major complication following rib resection is the development of a flail chest, and resection of more than six ribs is not recommended. We performed a resection with six ribs for a liposarcoma in the thoracic wall of a dog, followed by reconstruction of two cephalic ribs with a titanium plate. There was no postoperative flail chest and the patient’s respiratory status was stable. Rib reconstruction with plates was considered to allow functional reconstruction of the thoracic wall after extensive mass resection.</p>

DOI： 10.2327/jjvas.54.53

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ani13193054

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DOI： 10.21873/invivo.13415.

DOI： 10.21873/invivo.13415.

Other Link： https://click.endnote.com/viewer?doi=10.21873%2Finvivo.13415&token=WzM2ODE4MzUsIjEwLjIxODczL2ludml2by4xMzQxNSJd.eVWli9SZGYGRjlemNthfE20t13s

Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/vetsci10090533

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Various microRNAs (miRNAs) present in autologous blood products of canines have not been studied recently. We aimed to elucidate the existence of miRNAs in platelet-rich fibrin (PRF) and the stability of canine autologous blood products under various storage conditions. Total RNAs were isolated from PRF and other autologous blood products following newly adapted protocols used in commercial kits for plasma and tissue samples. Quantitative real-time polymerase chain reaction analysis (qPCR) was used to detect miRNAs in autologous blood products. The miR-16, miR-21, miR-155, and miR-146a were abundant in PRF and other autologous blood products of canines. Furthermore, we found they could maintain stability under protracted freezing temperatures of −30 °C for at least one month. Our findings revealed that PRF might be a stable resource for various canine miRNAs.

DOI： 10.3390/ijms24010770

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Language：Japanese   Publishing type：Research paper (scientific journal)  

Case summary: An 8-year-old neutered male domestic shorthair cat was presented for further investigation of anorexia, vomiting and lethargy. Abdominal ultrasonography and contrast-enhanced CT revealed choledocholithiasis with suspected bacterial peritonitis and non-visualisation of the gallbladder. During surgery, the common bile duct was noted to be perforated, and a cholelith was found in the abdominal cavity. No gallbladder was confirmed during surgery. Three months postoperatively, the cat underwent CT cholangiography and absence of the gallbladder with a vestigial duplicated gallbladder was diagnosed. Relevance and novel information: Gallbladder agenesis is extremely rare in cats, with only one previous report, but several dogs have been diagnosed based on CT cholangiography and laparoscopy. This report describes gallbladder agenesis concurrent with choledocholithiasis in an adult cat and represents the first report of CT cholangiography in a cat with gallbladder agenesis.

DOI： 10.1177/20551169221146513

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

Heart failure cause hypoperfusion-induced damage to abdominal organs due to decreased cardiac output (CO). Using a model dog with heart failure caused by rapid ventricular pacing (RVP), we have previously demonstrated that a decrease in CO reduces pancreatic blood flow (PBF). Furthermore, we have revealed that pancreatic acinar cell atrophy, which is a change in the pre-stage of pancreatitis was caused. However, the mechanism by which pancreatic acinar cell atrophy was caused in RVP dogs remains unknown. This study aimed to clarify the association between cardiac function, PBF, and histopathological changes in pancreatic acinar cells by administrating pimobendan, which increase CO, to RVP dogs. RVP dogs were divided into the control group (no medication, n = 5) and the pimobendan group (pimobendan at 0.25 mg/kg BID, n = 5). Non-invasive blood pressure measurement, echocardiography, and contrast-enhanced ultrasonography for PBF measurement were performed before initiating RVP and at 4 weeks after initiating RVP (4 weeks). At 4 weeks, the decreases in CO, mean blood pressure and PBF due to RVP were suppressed in pimobendan group. Furthermore, histopathological examination showed no changes in pancreatic acinar cells in the pimobendan group. Overall, it was clarified that the decrease in PBF due to cardiac dysfunction was a direct cause of pancreatic acinar cell atrophy. This suggests that maintaining PBF is clinically important for treating dogs with heart failure. In addition, these findings offer a reliable basis for developing new therapeutic strategies for heart failure in dogs, that is, pancreatic protection.

DOI： 10.3389/fvets.2022.925847

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Authorship：Last author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Anticancer Research USA Inc.  

Background/Aim: Canine mammary gland tumors (MGTs), as a potential model of human breast cancer, have a well-defined histological classification system. MicroRNA (miRNA) expression is a key part of the molecular signatures of both MGTs and human breast cancer, although the signatures alone do not yet provide a sufficient basis for definitive diagnosis. In this study, we investigated the association between miRNA expression patterns and histological classification. Materials and Methods: Mammary gland tissue was collected from healthy dogs (n=7) and dog patients (n=80). Further samples (n=5) were obtained from established MGT cell lines. We targeted miRNAs differentially expressed in metastatic tumor tissue versus non-metastatic and normal tissue. A subset of samples was analyzed using small RNA next generation sequencing (NGS) with subsequent qPCR. Results: Six differentially expressed miRNAs were selected from the NGS analysis and submitted for large-scale qPCR. The large-scale qPCR analysis revealed greater alternations in miRNA expression. Large-scale analysis, based on 79 samples, revealed a hierarchical clustering based on selected miRNAs that did not strikingly match the histopathological subtype classification. Conclusion: We successfully investigated the large-scale miRNA expression pattern in canine MGT and provided the whole miRNA expression. The selected miRNA demonstrated that there is no straightforward mapping between molecular signatures and histological classification of canine MGTs at the miRNA level.

DOI： 10.21873/invivo.12873

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Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons.

Pigs were allocated to summer- and winter-finishing cohorts, 12 each. The evaluations included assessment of carcass traits and internal organs’ normality carried out at the time of slaughter, and measurement of biomarkers in whole blood: derivatives of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential as markers of oxidative stress, and serum amyloid A and albumin/globulin (A/G) ratio as markers of acute and chronic inflammation, respectively.

The summer-finished pigs reared under subtropical field conditions showed lower carcass quality than the winter-finished pigs, indicating a potential adverse effect of summer temperatures on the swine industry. Marginal changes were observed in d-ROMs and the A/G ratio between the summer- and winter-finishing cohorts.

The results demonstrate that d-ROMs and the A/G ratio could be used as sensitive markers for heat stress under field conditions.

Keywords
albumin/globulin ratioderivatives of reactive oxygen metabolites (d-ROMs)biological antioxidant potential (BAP)heat stressKagoshima Berkshire pigoxidative stress

DOI： 10.2478/jvetres-2022-0024

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE  

<p>A 36-day-old Japanese Black calf exhibited wheezing associated with dyspnea from birth. Arterial blood gas analysis revealed a low oxygen partial pressure of 51 mmHg, low oxygen saturation of 83%, and high carbon dioxide partial pressure of 58.8 mmHg. Computed tomography, endoscopy, and ultrasonography showed cyst formation under the epiglottis. When the cyst was aspirated under ultrasonic guidance to secure the airway, 30 ml of viscous white turbid content was aspirated. The cyst shrank immediately after aspiration, but the wheezing and respiratory symptoms resumed 7 days after aspiration. Therefore, the cyst was surgically removed from the ventral side of the neck. No cyst remodeling was observed 30 days after surgical removal.</p>

DOI： 10.1292/jvms.22-0183

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Authorship：Last author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

Canine mammary gland tumors are very common and represent a potential model of human breast cancer, and microRNA (miRNAs) are promising biomarkers and therapeutic targets for these tumors. Accordingly, we aimed to identify miRNAs differentially expressed in canine mammary gland tumors using next generation sequencing (NGS), with subsequent confirmatory qPCR and target gene analyses. Mammary gland tissue was collected from healthy dogs (n=7) and dogs with suspected tumors (n=80). A subset of samples was analyzed with NGS to identify differentially expressed miRNAs with CLC Genome Workbench. Normal (n=10), tumor-adjacent (n=6), and tumor-bearing (n=76) mammary gland tissue samples were analyzed for the identified miRNAs using qPCR. An in silico analysis (TargetScan) was performed to predict the miRNAs’ target genes using gene ontology (GO) terms and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database (DAVID). We identified four miRNAs (cfa-miR-1-3p, cfa-miR-133a-3p, cfa-miR-133b-3p, and cfa-miR-133c-3p) as down regulated in canine mammary gland tumor tissues relative to normal and tumor adjacent tissues. KEGG analysis revealed the potential target genes of cfa-miR-1-3p are related to the Rap1 signaling pathway, adherens junction, and Ras signaling pathway, and those of the miR-133 family are related to the TGF-beta signaling pathway, synaptic vesicle cycle, and sphingolipid signaling pathway. In combination, these target genes are related to the regulation of transcription and DNA binding transcription (GO analysis), and the Hippo signaling pathway, adherens junction, and endocytosis (KEGG analysis). Accordingly, we suggest these four miRNAs are promising potential biomarker candidates for canine mammary gland tumors warranting further investigation.

DOI： 10.1016/j.gene.2022.146237

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Authorship：Last author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

Novel small non-coding RNAs (sRNAs) represent an emerging line of research in both human and canine oncology, due to their diverse regulatory and functional roles. Novel sRNAs are regarded as distinct from microRNAs, although both are part of the exosomal cargo. Recently, we reported on exosomal miRNAs as biomarkers for canine melanoma; however, it is unknown if novel sRNAs hold similar potential. Accordingly, we aimed to identify and validate novel sRNAs as potential biomarkers of canine oral melanoma, as part of our larger project on sequencing small exosomal RNA for this disease. Next generation sequencing revealed several differentially expressed novel sRNAs in exosomes from two melanoma cell lines (KMeC and LMeC) when compared with reference exosomes (from tumour-free dogs). Among these novel sRNAs, long noncoding RNA fragments, tRNA-derived fragments, snoRNAs and snRNAs were abundantly expressed. We selected four novel sRNAs upregulated in each cell line, and validated their aberrant expression with qPCR. In analysis using plasma-derived exosomes from melanoma patients, six out of the eight selected novel sRNAs showed significantly elevated expression. Receiver operating curve (ROC) analysis showed that one long non-coding RNA-derived small fragment (ENSCAFT00000069599.1) and one transfer RNA-derived small fragment (tRNA-Ala-TGC-5-1) have more than 85% sensitivity and specificity for differentiating melanoma patients from tumour-free dogs. Therefore, we consider that novel sRNAs may serve as candidate biomarkers to facilitate more accurate diagnosis of canine oral melanoma in clinical settings.

DOI： 10.1111/vco.12818

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Objectives : To characterise the clinical signs of suspected cerebrovascular disease in dogs. Materials and Methods : Medical records of one hospital were searched from November 2009 to December 2016 for dogs that suffered of cerebrovascular disease. We diagnosed cerebrovascular disease based on acute onset, clinical signs and magnetic resonance imaging findings. The medical history, clinical signs, concurrent disease, area of infarction, cerebrospinal fluid results, month at onset and outcome were investigated in the cerebrovascular disease group and in a control group (dogs with brain disorders other than cerebrovascular disease). Results : A total of 122 CVD cases were extracted from the 5312 patients that visited during the study period. Of these 122 cases, 66 (1.2%) matched the subject selection criteria of our study and were included in the analysis. Forebrain infarction was observed in 51 of 66 cases, of which 24 (47.1%) suffered from seizures. The number of dogs diagnosed with cerebrovascular disease was disproportionately high in August (nine of 59 cases) and December (13 of 59 cases). In the outcome survey, deterioration was observed in 11 of 55 cases. Clinical Significance : Seizure is an important clinical sign of cerebrovascular disease in dogs. There was a significant seasonal variation in the number of dogs diagnosed with cerebrovascular disease in Japan. Clinical features observed in this report differ from those of previous reports and highlight the need for additional research in this area.

DOI： 10.1111/jsap.13422

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Considering the importance of the canine cancer model of human disease, as well as the need for strategies for canine cancer management, the properties of exosomes are an emerging topic in canine oncology. In our study, exosomal RNA was isolated and investigated by next-generation sequencing. We identified several differentially expressed microRNAs (miRNAs/miRs) in the exosomes of two melanoma cell lines compared with non-tumor reference exosomes. We explored these potential melanoma-specific exosomal miRNAs further and found that miR-143 and let-7b increased in primary, whereas miR-210, 708, 221, and 222 increased in metastatic site originated melanoma cells. Further analysis showed miR-143 and 221 significantly increased in plasma exosomes of metastatic melanoma patients. Moreover, the sensitivity and specificity are >85% for differentiating the non-metastatic and metastatic patients. Therefore, these miRNAs can be an incredible biomarker candidate to identify metastatic melanoma and facilitate a better prognosis.

DOI： 10.1111/pcmr.13000

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

MicroRNAs (miRNAs) can rapidly respond to cellular stresses, such as hypoxia. This immediate miRNA response regulates numerous genes and influences multiple signaling pathways. Therefore, identifying hypoxia-regulated miRNAs (HRMs) is important in canine oral melanoma (COM) to investigate their clinical significance. The hypoxic and normoxic miRNA profiles of two COM cell lines were investigated by next generation sequencing. HRMs were identified by comparing miRNA expression profiles in these cell lines with that in COM tissue. The HRM profile was different between cell lines of primary and metastatic origin, except for miR-301a and miR-8884. The time course of miRNA expression determined by qRT-PCR, especially for miR-210 and miR-301a, showed that metastatic cells are more resistant to hypoxia than primary cells. Analysis of an experimentally validated human miRNA target database revealed that miR-21 and miR-301a control a complex gene regulatory network in response to hypoxia, which includes pathways of well-known oncogenes, such as VEGF, PTEN, and TGFBR2. In conclusions, we revealed the HRM of COM. Moreover, our study shows the difference in regulation and response of hypoxic miRNAs between primary and metastatic originated melanoma cells.

DOI： 10.1016/j.gene.2021.145552

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE  

In dogs, pancreatic acinar cell injury is thought to be caused by decreased pancreatic blood flow due to heart failure. In previous our report, it demonstrated that decreased heart function causes a significant decrease in pancreatic blood flow in heart failure dog model caused by rapid ventricular pacing (RVP). However, the types of histopathological changes remain unclear. We aimed to verify the types of histopathological changes occurring in the pancreatic tissue due to decreased heart function. After RVP for 4 weeks, atrophy of pancreatic acinar cells, characterized by a decrease in zymogen granules, was observed in all areas of the pancreas. In conclusion, the result of this study suggests that attention should be paid to ischemia/hypoperfusion injury in the pancreas.

DOI： 10.1292/jvms.20-0409

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1177/1040638721991862

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Anticancer Research USA Inc.  

DOI： 10.21873/INVIVO.12244

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DOI： 10.3892/br.2020.1397

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DOI： 10.1016/j.rvsc.2020.10.001

Other Link： https://www.ncbi.nlm.nih.gov/pubmed/33162110

Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

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DOI： 10.21873/invivo.11982

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers in Veterinary Science  

DOI： 10.3389/fvets.2020.00307

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/jvim.15768

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/vco.12558

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/febs.15114

Other Link： https://www.ncbi.nlm.nih.gov/pubmed/31663680

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3892/or.2019.7391

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/ijms20194832

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1177/0300985819839236

Publisher：(公社)日本実験動物学会  

微少循環系の血流の血栓形成を総合的に評価できる総合的血栓形成評価システム(T-TAS)を用いて、心房細動を模したイヌ実験モデルの急速心房ペーシング(RAP)直後の心房内と末梢領域における血液凝固を比較した。その結果、ペーシング30分後の心房血では、ARチップ(凝固系と血小板のミックスした血栓分析のためのチップ)による+10kPaまでの凝固機能評価時間(T10)と閉塞時間(OT)の両方が、ペーシング前に比べて有意に短縮した。ペーシング30分後の心房血のPLチップ(血小板血栓分析のためのチップ)によるOTは、ペーシング前に比べて有意に短縮した。一方、末梢血ではARチップとOTチップによる、T10でもOTでもRAPによって有意な変化はなかった。以上、RAP発症30分後には血液凝集の上昇が示唆されたことから、末梢血での凝集性に変化がなかったとしても、心房細動後早期に抗血栓治療を始めたほうが良いと考えられた。

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3892/ol.2018.9692

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1538/expanim.18-0100

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1186/s12917-018-1611-0

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.thromres.2018.09.040

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/978-3-319-91287-5_14

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Veterinary and Comparative Oncology  

DOI： 10.1111/vco.12379

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DOI： 10.21873/invivo.1127

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Large Animal Clinics / Large Animal Clinic Research Association  

<p>　In recent years, diagnostic imaging has advanced in the veterinary field, and digital radiography has become more common in livestock animals. This review summarizes image characteristics obtained by different imaging parameters and the pitfalls of image interpretation using actual clinical cases.</p>

DOI： 10.4190/jjlac.8.269

Publisher：(株)緑書房  

Publisher：(一社)日本動脈硬化学会  

マイクロミニピッグ(μMP)を用いて、肥満、血清脂質代謝、アテローム性動脈硬化、肝傷害、肝臓、全身マクロファージ活性化に対するコール酸(CA)の影響を評価した。μMPを高脂肪/高コレステロール食(HcD)群、HcDに0.7%CAを添加(HcD+CA)群に分け、8週間飼育した。飼育前後に腹部CTを行い、血糖値、血球数、血液化学、リポ蛋白質値、血圧を測定した。全器官を切除して組織学的および免疫組織化学検査を行った。mRNA発現をリアルタイムPCRで定量した。HcD+CA群はHcD群よりも内臓脂肪症、アテローム性動脈硬化の進行、高血清酸化ストレスマーカー値を示したが、血清脂質値は同じであった。肝臓では脂質蓄積が増加し、酸化ストレスマーカーの発現が高く、泡沫性クッパー細胞と星状細胞の活性化が促進し、肝細胞アポトーシスが増加し、非アルコール性脂肪性肝疾患(NAFLD)を示した。HcD+CA群では網内系、肺毛細血管、皮膚を含む種々の器官で泡沫性マクロファージの動員が観察された。CAは局所的および全身での酸化ストレスと炎症性サイトカインを発現亢進させ、種々の器官でのマクロファージを活性化させ、内臓型肥満、アテローム性動脈硬化、NAFLDを促進させた。

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.2527/jas.2017.1384

Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1292/jvms.16-0457

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.mvr.2017.06.001

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pone.0177182

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pone.0177305

Publisher：HINDAWI LTD  

The combination of alteplase, a recombinant tissue plasminogen activator, and edaravone, an antioxidant, reportedly enhances recanalization after acute ischemic stroke. We examined the influence of edaravone on the thrombolytic efficacy of alteplase by measuring thrombolysis using a newly developed microchip-based flow-chamber assay. Rat models of embolic cerebral ischemia were treated with either alteplase or alteplase-edaravone combination therapy. The combination therapy significantly reduced the infarct volume and improved neurological deficits. Human blood samples from healthy volunteers were exposed to edaravone, alteplase, or a combination of alteplase and edaravone or hydrogen peroxide. Whole blood was perfused over a collagen- and tbromboplastin-coated microchip; capillary occlusion was monitored with a video microscope and flow-pressure sensor. The area under the curve (extent of thrombogenesis or thrombolysis) at 30 minutes was 69.9% lower in the edaravone-alteplase- than alteplase-treated group. The thrombolytic effect of alteplase was significantly attenuated in the presence of hydrogen peroxide, suggesting that oxidative stress might hinder thrombolysis. D-dimers were measured to evaluate these effects in human platelet-poor plasma samples. Although hydrogen peroxide significantly decreased the elevation of D-dimers by alteplase, edaravone significantly inhibited the decrease. Edaravone enhances alteplase-mediated thrombolysis, likely by preventing oxidative stress, which inhibits fibrinolysis by alteplase in thrombi.

DOI： 10.1155/2017/6873281

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本農芸化学会  

マイクロミニブタに8週間腸溶性ラクトフェリン(eLF)を経口摂取させたところ、高脂肪高コレステロール食(HFCD)による血清の総コレステロール値とLDL-コレステロール値の上昇が著しく減弱したが、HDL-コレステロールは変化しなかった。血管の組織検査より、eLFにより全身の粥状動脈硬化の進行が阻害されていた。eLF投与に特徴的な肝臓の遺伝子による経路分析では、ステロイド生合成経路に有意な変化が起こり、これらの遺伝子はすべて上方制御されていた。すなわち、HFCDにより阻害されたコレステロール合成がeLFにより回復したことが示唆された。以上より、eLFはHFCDによるコレステロール代謝異常からホメオスタシスを防御することにより、高コレステロール血症と粥状動脈硬化症を防ぐことが示唆された。

Language：English   Publishing type：Research paper (scientific journal)   Publisher：(株)ファームプレス  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

In recent years, animals are increasingly being transported by vehicle. The mitigation of stress animals aresubjected to while being transported by vehicle is desirable from the perspective of animal welfare. Weinvestigated the potential of acupuncture, which has fewer side effects than drug therapy, as a means fortreating canine transportation stress. Eleven dogs which had exhibited motion sickness symptoms duringtransportation (vomiting, salivation, and a reduced amount of spontaneous activity) underwent an easy-to-applyacupuncture treatment. Short, ultra-thin circular transdermal needles were applied to locations correspondingto acupoints on the apical area of both ears, which are identified as Erjian Points 1 to 3 (“Jisen” in Japanese) intraditional oriental medicine. Motion sickness symptoms were suppressed in the treated dogs the next timethey were transported. We consider acupuncture to be a promising potential therapeutic strategy in veterinarymedicine.

DOI： 10.12935/jvma.69.143

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Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2016&ichushi_jid=J01104&link_issn=&doc_id=20160318440004&doc_link_id=10.12935%2Fjvma.69.143&url=https%3A%2F%2Fdoi.org%2F10.12935%2Fjvma.69.143&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

Mediastinal mass and hepatomegaly were detected by computed tomography in a 10-month-old Miniature Dachshund presenting with dyspnea. The dog was diagnosed as mediastinal T-cell lymphoma with liver invasion by cytological and clonality examination. Multidrug therapy (UW-25) was not effective for the dog. As a second-line therapy, a combination treatment of toceranib phosphate and CCNU was performed based on the expression profiling of multidrug resistance genes and angiogenic genes by qRT-PCR analysis, resulting in an improved condition. However, the dog died from dyspnea at 36 days after first examination. T-cell lymphoma was confirmed by necropsy.

DOI： 10.12935/jvma.69.333

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

Eight Japanese black calves (14‐65 days old) with severe respiration insufficiency due to tracheal stenosis by rib fractures at the calving were brought to Kagoshima University Veterinary Teaching Hospital. At the first visit (NOSAI), all calves had common symptoms of coughing and wheezing sounds. All calves were towed at the time of calving. In x-ray and computed-tomography (CT) examinations, the 1st to 6th right and/or left ribs were fractured and tracheal stenosis was observed. Surgical treatment was conducted in all cases to remove the 1st to 3rd fractured ribs on one side that compressed the trachea. The respiratory condition of all calves improved from the day after surgical relocation and had completely reversed by the 14th postoperative day. From the above, CT examination was found to be useful for diagnosing tracheal stenosis due to rib fracture while removing the fractured ribs on one side is effective surgical treatment.

DOI： 10.12935/jvma.69.267

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：(一社)日本動脈硬化学会  

生後3ヵ月で体重が僅か5kgの世界最小のマイクロミニブタ(MMP)を用いて脂質異常症誘発性動脈硬化の動物モデルを開発し、脂質代謝とアテローム形成に関するヒトとの類似性について調べた。MMPにコール酸以外のコレステロールを0.2%以上含む高脂肪/高コレステロール食を8週間与えたところ、高コレステロール血症となり、動脈硬化を発症した。血清のLDL-C値とLDL画分の割合は著しく増加し、LDL受容体とHMG-CoA還元酵素の肝発現は協調的に減少した。MMPの血清よりコレステロール輸送に逆の働きを及ぼすコレステリルエステル転送蛋白質活性を検出した。Niemann-Pick C1様1蛋白は肝臓と小腸で発現したが、肝性apoB mRNA編集酵素は発現しなかった。ヒトと同様、そしてマウスとは対照的に、肝性リパーゼ活性の大半は肝臓に局在していた。

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPANESE PHARMACOLOGICAL SOC  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPAN ATHEROSCLEROSIS SOC  

Aim: Experimental studies of human atherogenesis require an appropriate animal model that mimics human physiology and pathology. Because swine physiology is similar to human physiology, we developed a hyperlipidemia-induced atherosclerosis model using the recently developed world's smallest Microminipig (TM).
Methods: These animals weigh only 5 kg at 3 months of age, much smaller than any other miniature pig. We found that the administration of a high-fat/high-cholesterol diet containing at least 0.2% cholesterol without cholic acid for as little as eight weeks induces hypercholesterolemia and subsequent atherosclerosis in these animals.
Results: The serum levels of low-density lipoprotein cholesterol (LDL-C) and the percent distribution of cholesterol in the LDL fractions were markedly increased. The hepatic expression of LDL receptor and hydroxymethylglutaryl-CoA reductase was coordinately decreased. The cholesteryl ester transfer protein activity, which plays a role in reverse cholesterol transport, was detected in the serum of the Microminipigs. Niemann-Pick C1-like 1 protein was expressed in both the liver and small intestine; however, hepatic apoB mRNA editing enzyme was not expressed. As in humans, and in contrast to that observed in mice, most of the hepatic lipase activity was localized in the liver. These results suggest that the hyperlipidemia-induced gene expression profile linked to cholesterol homeostasis and atherogenesis is similar in Microminipigs and humans.
Conclusion: We conclude that the characteristics of the Microminipig, including its easy handling size, make it an appropriate model for studies of atherosclerosis and related conditions.

DOI： 10.5551/jat.21246

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

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DOI： 10.3892/br.2012.7

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN  

High residual platelet aggregability and circulating platelet-monocyte aggregates in patients administered aspirin and clopidogrel are associated with ischaemic vascular events. To determine the relevance of these factors with residual thrombogenicity, we measured platelet thrombus formation using a microchip-based flow-chamber system in cardiac patients receiving aspirin and/or clopidogrel, and evaluated its correlation with agonist-inducible platelet aggregation and platelet-monocyte aggregates. Platelet thrombus formation was analysed by measuring flow pressure changes due to the occlusion of micro-capillaries and was quantified by calculating AUC(10) (area under the flow pressure curve). The growth and stability of platelet thrombi that formed inside microchips at shear rates of 1000, 1500, and 2000 s(-1) were markedly reduced in patients receiving aspirin and/or thienopyridine compared to healthy controls (n=33). AUC(10) values of aspirin therapy patients (n=20) were significantly lower and higher than those of healthy controls and dual antiplatelet therapy patients (n=19), respectively, and showed relatively good correlations with collagen-induced platelet aggregation and platelet-monocyte aggregates at 1000 and 1500 s(-1) (r(s)&gt;0.59, p&lt;0.01). In contrast, AUC(10) in dual antiplatelet therapy patients was significantly correlated with ADP-induced platelet aggregation at all examined shear rates (r(s)&gt;0.59, p&lt;0.01), but did not correlate with collagen-induced aggregation. Aspirin monotherapy patients with high residual platelet thrombogenicity also exhibited significant elevations in both collagen-induced platelet aggregation and platelet-monocyte aggregates. Our results, although preliminary, suggest that residual platelet thrombogenicity in aspirin-treated patients is associated with either collagen-induced platelet aggregation or circulating platelet-monocyte aggregates, but it is predominantly dependent on ADP-induced platelet aggregation in patients receiving dual antiplatelet therapy.

DOI： 10.1160/TH12-06-0441

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Pet Animal Nutrition  

Previously, we reported that Kagoshima, Japan, is an endemic area of canine silica urolithiasis. Tap water in Kagoshima contains significantly greater silica concentrations than other areas of Japan. Ingestion of silica from tap water may lead to the formation of silica uroliths in dogs. We measured the concentration of silica in tap water from various areas of Japan. Concentrations were higher in the city of Takeda（Oita prefecture） and in some areas around the city of Chikusei（Ibaraki prefecture）. Fast atom bombardment mass spectrometry analysis of water from Kirishima（Kagoshima prefecture）revealed that the character of water was similar to that after silica sintering. Removal of silica from tap water may decrease the risk of silica urolithiasis in dogs. We demonstrated that silica could be successfully removed from tap water with a reverse osmosis membrane water filter but not with a typical domestic water filter. We also sent internet-based questionnaires to owners of dogs with silica uroliths to investigate other possible etiologies of silica urolithiasis. The results revealed that the specific pet food brand may be related to silica urolithiasis. However, the concentration of total silica in pet food does not appear to be related to the formation of uroliths.

DOI： 10.11266/jpan.16.61

Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

Stroke is a major cause of mortality and disability worldwide. The main cause of stroke is atherosclerosis, and the most common risk factor for atherosclerosis is hypertension. Therefore, antihypertensive treatments are recommended for the prevention of stroke. Three angiotensin receptor blockers (ARBs), telmisartan, irbesartan and candesartan, inhibit the expression of the receptor for advanced glycation end-products (RAGE), which is one of the pleiotropic effects of these drugs. High mobility group box 1 (HMGB1) is the ligand of RAGE, and has been recently identified as a lethal mediator of severe sepsis. HMGB1 is an intracellular protein, which acts as an inflammatory cytokine when released into the extracellular milieu. Extracellular HMGB1 causes multiple organ failure and contributes to the pathogenesis of hypertension, hyperlipidemia, diabetes mellitus, atherosclerosis, thrombosis, and stroke. This is the first review of the literature evaluating the potential of three ARBs for the HMGB1-RAGE axis on stroke therapy, including prevention and acute treatment. This review covers clinical and experimental studies conducted between 1976 and 2013. We propose that ARBs, which inhibit the HMGB1/RAGE axis, may offer a novel option for prevention and acute treatment of stroke. However, additional clinical studies are necessary to verify the efficacy of ARBs.

DOI： 10.3390/ijms140918899

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(株)ファームプレス  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：(一社)日本ペット栄養学会  

シリカ結石はイヌではまれな尿石とされている。今回、我々は鹿児島県を中心にシリカ尿結石について疫学調査を行った。その結果、鹿児島県内の獣医師やオーナーに対するアンケート調査において、鹿児島県を中心とする地域でシリカ尿結石が多くみられ、その発生の要因として飲料水との関係が示唆された。そこで鹿児島県内の水道水中のシリカ濃度を測定したところ、他地域と比較して著しく高値を示した。またミネラルウォーターについても同様に、鹿児島県が採水地の製品でシリカ濃度が高値のものがあった。実験的にシリカを高濃度に含む水道水の飲水により尿中シリカ濃度の上昇は見られなかったが、疫学調査から、シリカ濃度の低い水を飲水として使用することがシリカ結石発生の予防となると推測した。(著者抄録)

Language：English   Publishing type：Research paper (scientific journal)   Publisher：(株)エデュワードプレス  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Pet Animal Nutrition  

Silica calculus is unusual in urolithiasis in dogs. We conducted epidemiological study on silica calculus in dogs around Kagoshima area. Information questionnaires from veterinarians and clients indicated that silica urolithiasis was not related to food and dog's habit (eating clod), but was associated with drinking tap water. Silica concentration of tap water in Kagoshima area was obviously higher than that in another area in Japan. A brand of mineral water made in Kagoshima area also had high silica level. Though intake of high level silica in water did not affect urine silica concentration experimentally, our results indicated that it is beneficial to use low silica level water to prevent silica urolithiasis in dogs.

DOI： 10.11266/jpan.15.7

Language：English   Publishing type：Research paper (scientific journal)   Publisher：日本ペット栄養学会  

シリカ結石はイヌでは比較的まれな尿石とされている。昨年の本学会で、我々は鹿児島県ではシリカ尿結石が多くみられることを報告した。そして、鹿児島県内の水道水中のシリカ濃度は他の地域に較べ著しく高く、シリカ結石発生の要因として飲料水との関係を示唆した。しかし、新たな謎も生じた。鹿児島市内の水道水のシリカ濃度は比較的高いのになぜシリカ結石がほとんど生じないか、シリカの好発地帯ではみな同じ水を飲んでいるのにもかかわらずなぜ一部の犬でシリカ結石が生じるのか、さらに、シリカ好発地域以外では何が原因でシリカ結石が発症するのか、などである。今回はそれらの謎に答えるため各種実験を行い、シリカ発生の機序とその予防法について考察した。

DOI： 10.11266/jpan.15.Suppl_44

Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPANDIDOS PUBL LTD  

Free radicals play an important role in the pathogenesis of a variety of diseases; thus, they are an attractive target for therapeutic intervention in these diseases. Compounds capable of scavenging free radicals have been developed for this purpose and some, developed for the treatment of cerebral ischemic stroke, have progressed to clinical trials. One such scavenger, edaravone, is used to treat patients within 24 h of stroke. Edaravone, which can diffuse into many disease-affected organs, also shows protective effects in the heart, lung, intestine, liver, pancreas, kidney, bladder and testis. As well as scavenging free radicals, edaravone has antiapoptotic, anti-necrotic and anti-cytokine effects in various diseases. Here, we critically review the literature on its clinical efficacy and examine whether edaravone should be considered a candidate for worldwide development, focusing on its effects on diseases other than cerebral infarction. Edaravone has been safely used as a free radical scavenger for more than 10 years; we propose that edaravone may offer a novel treatment option for several diseases.

DOI： 10.3892/etm.2011.352

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：The Japan Society of Neurotraumatology  

DOI： 10.32187/neurotraumatology.34.2_225

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Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

An equine case of hind lameness and wobbler syndrome following an accidental forward fall during exercise was evaluated by diagnostic imaging. The myelography under general anesthesia was suggestive of a dorsally compressed spine between the sixth cervical vertebrae (C6) and C7. Histopathological findings also pointed to compression myelopathy between C6 and C7. A computed tomography scan of the cervical and thoracic (T) vertebraes following euthanasia was diagnostic of fractures of the C7 and joint space widening at the articular processes at the range from C6 to T2. Deformed alignment from C6 to T2 could induce spinal compression.

DOI： 10.12935/jvma.64.703

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Other Link： http://agriknowledge.affrc.go.jp/RN/2010814357

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：日本ペット栄養学会  

シリカ結石はイヌではまれな尿石とされている。今回、我々は鹿児島県を中心にシリカ尿結石について疫学調査を行った。その結果、鹿児島県内の獣医師やオーナーに対するアンケート調査において、鹿児島県を中心とする地域でシリカ尿結石が多くみられ、その発生の要因として飲料水との関係が示唆された。そこで鹿児島県内の水道水中のシリカ濃度を測定したところ、他地域と比較して著しく高値を示した。またミネラルウォーターについても同様に、鹿児島県が採水地の製品でシリカ濃度が高値のものがあった。実験的にシリカを高濃度に含む水道水の飲水により尿中シリカ濃度の上昇は見られなかったが、疫学調査から、シリカ濃度の低い水を飲水として使用することがシリカ結石発生の予防となると推測した。

DOI： 10.11266/jpan.14.Suppl_73

Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPANDIDOS PUBL LTD  

Nosocomial infections caused by microbial opportunistic infections or microbial biofilms may occur during hospitalization and increase patient morbidity, mortality and health care costs. Artificial antibiotic agents were initially used to prevent infection; however, the high prevalence of nosocomial infections has resulted in their excessive use, which has led to microbial resistance to these agents. The increase in microbial resistance to antibiotics and the development of antibiotic agents may be the cause of the production of other microbial resistance. Thus, natural compounds that have no adverse side effects would be a preferred treatment modality. Recently, the monosaccharide 1,5-anhydro-D-fructose (1,5-AF), a natural plant compound derived from starch, has been found to have multifunctional properties, including antioxidant, antiplatelet aggregation by thrombin and anti-inflammatory activities. The results of the present study demonstrate that 1,5-AF suppressed the growth of coagulase-negative staphylococci on the hands as well as the growth of Staphylococcus epidermidis, which is a cause of opportunistic infections. Furthermore, 1,5-AF suppressed biofilm formation by the methicillin-resistant Staphylococcus aureus. In conclusion, 1,5-AF is a natural compound that may be effective in preventing nosocomial infections, without causing adverse side effects.

DOI： 10.3892/etm.2011.245

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Edaravone was originally developed as a potent free radical scavenger and has been widely used to treat cerebral infarction in Japan since 2001. Several free radical scavengers have been developed and some of them have progressed to clinical trials for the treatment of cerebral infarction. One such scavenger, edaravone, has been approved by the regulatory authority in Japan for the treatment of patients with cerebral infarction. Of particular interest is the ability of edaravone to diffuse into the central nervous system in various neurologic diseases. Aside from its hydroxyl radical scavenging effect, edaravone has been found to have beneficial effects on inflammation, matrix metalloproteinases, nitric oxide production and apoptotic cell death. Concordantly, edaravone has been found to have neuroprotective effects in a number of animal models of disease, including stroke, spinal cord injury, traumatic brain injury, neurodegenerative diseases and brain tumors. The proven safety of edaravone following 9 years of use as a free radical scavenger suggests that it may have potential for development into an effective treatment of multiple neurologic conditions in humans.

DOI： 10.3892/etm.2011.281

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Historically, clinical outcomes following spinal cord injury (SCI) have been dismal. Severe SCI leads to devastating neurological deficits, and there is no treatment available that restores the injury-induced loss of function to a degree that an independent life can be guaranteed. To address all the issues associated with SCI, a multidisciplinary approach is required, as it is unlikely that a single approach, such as surgical intervention, pharmacotherapy or cellular transplantation, will suffice. High mobility group box 1 (HMGB1) is an inflammatory cytokine. Various studies have shown that HMGB I plays a critical role in SCI and that inhibition of HMGB1 release may be a novel therapeutic target for SCI and may support spinal cord repair. In addition, HMGB1 has been associated with graft rejection in the early phase. Therefore, HMGB1 may be a promising therapeutic target for SCI transplant patients. We hypothesize that inhibition of HMGB1 release rescues patients with SCI. Taken together, our findings suggest that anti-HMGB1 monoclonal antibodies or short hairpin RNA-mediated HMGB1 could be administered for spinal cord repair in SCI patients.

DOI： 10.3892/etm.2011.310

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPANDIDOS PUBL LTD  

Free radicals play major roles in the pathogenesis of tissue damage in many diseases and clinical conditions, and the removal of free radicals may offer a treatment option. Several modulators of free radical scavenger pathways have been developed and some have progressed to clinical trials. One such agent, edaravone, was approved in 2001 in Japan for the treatment of cerebral infarction. It has since been shown that edaravone can diffuse into many organs and, in addition to its effects on hydroxyl radical removal, edaravone modulates inflammatory processes, matrix metalloproteinase levels, nitric oxide production, apoptotic cell death, and necrotic cell death. Edaravone also exerts protective effects in a number of animal models of disease and tissue damage, including models of myocardial, lung, intestinal, liver, pancreatic and renal injury. Together with the proven safety of edaravone following 9 years of use as a modulator of free radical scavenging pathways in neurological disease, these additional effects of edaravone suggest that it may offer a novel treatment for several non-neurological diseases and clinical conditions in humans.

DOI： 10.3892/ijmm.2011.795

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：The Japan Society of Neurotraumatology  

DOI： 10.32187/neurotraumatology.33.2_241

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Estimation of the postmortem interval (PMI) is one of the most important tasks in forensic medicine Numerous methods have been proposed for the determination of the time since death by chemical means High mobility group box-1 (HMGB1), a nonhistone DNA-binding protein is released by eukaryotic cells upon necrosis Postmortem serum levels of HMGB1 of 90 male Wistar rats stored at 4 14 and 24 degrees C since death were measured by enzyme-linked immunosorbent assay The serum HMGB1 level showed a time-dependent increase up to seven days at 4 degrees C At 14 degrees C, the HMGB1 level peaked at day 3, decreased at day 4, and then plateaued At 24 degrees C, the HMGB1 level peaked at day 2, decreased at day 3, and then plateaued Our findings suggest that HMGB1 is related to the PMI in rats

DOI： 10.3892/etm_00000019

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Background: C-reactive protein (CRP) is widely used as a sensitive biomarker for inflammation. Increasing evidence suggests that CRP plays a role in inflammation. High-mobility group box-1 (HMGB1), a primarily nuclear protein, is passively released into the extracellular milieu by necrotic or damaged cells and is actively secreted by monocytes/macrophages. Extracellular HMGB1 as a potent inflammatory mediator has stimulated immense curiosity in the field of inflammation research. However, the molecular dialogue implicated between CRP and HMGB1 in delayed inflammatory processes remains to be explored. Methods and results: The levels of HMGB1 in culture supernatants were determined by Western blot analysis and enzyme-linked immunosorbent assay in macrophage RAW264.7 cells. Purified CRP induced the release of HMGB1 in a dose- and time-dependent fashion. Immunofluorescence analysis revealed nuclear translocation of HMGB1 in response to CRP. The binding of CRP to the Fc gamma receptor in RAW264.7 cells was confirmed by fluorescence-activated cell sorter analysis. Pretreatment of cells with IgG-Fc fragment, but not IgG-Fab fragment, efficiently blocked this binding. CRP triggered the activation of p38MAPK and ERK1/2, but not Jun N-terminal kinase. Moreover, both p38MAPK inhibitor SB203580 and small interfering RNA significantly suppressed the release of HMGB1, but not the MEK1/2 inhibitor U-0126. Conclusion: We demonstrated for the first time that CRP, a prominent risk marker for inflammation including atherosclerosis, could induce the active release of HMGB1 by RAW264.7 cells through Fc gamma receptor/p38MAPK signaling pathways, thus implying that CRP plays a crucial role in the induction, amplification, and prolongation of inflammatory processes, including atherosclerotic lesions. (C) 2008 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.carpath.2007.08.006

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Objective: To determine if levels of the cartilage-specific (V + C)(-) fibronectin isoform in the synovial fluid is associated with cartilage change during osteoarthritis.
Design: Synovial fluid was collected from 26 healthy dogs presenting to the Orthopedic Surgery Clinic with unilateral cranial cruciate rupture, 22 control dogs, and 13 dogs from a colony maintained for the study of canine hip dysplasia. Total fibronectin, (V + C)(-) fibronectin, and cartilage oligomeric matrix protein (COMP) were quantitated by ELISA assays. Statistical analysis used Wilcoxon's signed-rank and rank-sum tests and Spearman's rank correlation.
Results: The concentration of total fibronectin was increased in affected (P &lt; 0.0001) and contralateral (P = 0.0005) knees of the clinic population (compared to unaffected knees in colony controls). Both (V + C)(-) fibronectin and COMP concentrations were elevated in the contralateral knees in clinical patients relative to unaffected knees in the colony controls (P = 0.03 and P = 0.04, respectively), and relative to the affected knees (P = 0.003); however, corrections for joint effusions suggest elevated totals in the affected knees. (V + C)(-) fibronectin and COMP concentrations were correlated (r(sp) = 0.74; P &lt; 0.0001) in 30 unaffected knees of patients and colony controls. Total fibronectin was correlated negatively with months since the initial injury (r(sp) = -0.44; P = 0.03) in the affected joints. The intraoperative lesion severity score did not correlate with total fibronectin or (V + C)(-) fibronectin (P greater than or equal to 0.35).
Conclusions: Concentration of total fibronectin in synovial fluid might be a useful biomarker for cross-sectional studies in osteoarthritis, but only (V + C)(-) fibronectin provides information specifically about cartilage damage. Elevated concentrations of (V + C)(-) fibronectin and COMP seen in the contralateral knees of patients with cranial cruciate rupture might indicate cartilage changes early in the disease process (pre-clinical). However, the wide range of values obtained limits the diagnostic value for any one individual. Joint effusions obscure the total amount of biomarkers in affected synovial joints. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.joca.2004.07.002

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPAN SOC VET SCI  

Permanent pacemakers are commonly used in veterinary practice and can have a dramatic effect on the treatment of heart block. A Labrador Retriever dog suffering from exercise intolerance secondary to third degree atrioventricular block was treated with a new pacemaker system. A steroid-eluting screw-in type lead that has the advantage of being more fixed to the myocardial wall without increasing the pacing threshold was used. The heart rate was regulated with an acceleration sensing pacemaker generator that included several automatic modulation systems. Nineteen months after implantation, the dog has a normal level of activity. The present case suggests that this pacemaker design may offer important advantages for canine patients.

DOI： 10.1292/jvms.65.1101

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本実験動物学会  

Clawnミニブタに心筋虚血を作製した.連続虚血群の3/8例が虚血後20分以内に,虚血再灌流群の4/9例が再灌流後に不整脈で死亡した.虚血領域は再灌流群の方が狭かった.再灌流障害は血行動態に重大な影響を与えることが示唆された

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

This study was designed to determine effects of trimming on hoof growth, hoof-disease occurrence, and beef productivity. Sixteen Japanese black steers (13.0&plusmn;2.0 months of age, 308.0&plusmn;30.8 kg in body weight) were divided into 4 groups: the no-trimming group and group 2; in which trimming was performed at 3-month intervals; group 3, in which trimming was performed at 6-month intervals; and group 4, in which trimming was performed at 12month-intervals. At 19-month intervals, hooves were measured and hoof appearance recorded.After slaughter, we made pathological investigations of hoof and joint diseases and obtained scores representing the quantity and quality of beef products. When trimming intervals were longer, hoof angle was smaller, and hoof wall and bulb height increased. The occurrence of hoof diseases in the no-trimming group was significantly higher (P<0.05) than in the other groups. Scores for beef-product quantity were significantly higher (P<0.05) in group 3, in which trimming was performed at 6-month intervals, than in the other groups. These results suggest that trimming may be useful in maintaining normal hoof growth and preventing deformation and disease.

DOI： 10.12935/jvma1951.54.829

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

The purpose of this study is to determine effects of hoof trimming on transport-induced stress in beef cattle. Forty-one Japanese black steers were randomly divided into 2 groups: trimmed group (n=27) and untrimmed group (n=14). In the trimmed group, hoof trimming was repeated every 6 months, with the final trimming performed 3 months before shipment. The untrimmed group was not trimmed at all until shipment time. Before and after truck transportation to the shipping destination (57.8 km, 4 hours), the cattle were weighed, and their blood was sampled for hematological and biochemical analysis. The indices of transport-induced stress were creatine phosphokinase (CK) and cortisol. In both groups, CK and cortisolincreased after transportation. Increase in the parameters was lower in the trimmed group than in the untrimmed group. Lower CK activity in the trimmed group suggests that the physical demand for posture support during transport in the vehicle was more restricted in thetrimmed group. Plasma cortisol increased less in the trimmed group than in the untrimmed group. This could mean that periodical trimming effectively inhibits transport-induced stress in beef cattle.

DOI： 10.12935/jvma1951.54.837

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医師会  

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医師会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

A three-year-old, female Shih Tzu with a history of dysphagia persisting since weaning had been kept on a liquid diet for about two years. The dog was referred to us because of difficulty in swallowing liquid food. Clinical signs, radiographic findings, and fluoroscopic examination after ingestion of food mixed with barium confirmed a diagnosis of cricopharyngeal achalasia. Four days later, we performed cricopharyngeal myotomy. After surgery, the dog no longer had difficulty swallowing food and was discharged on the third postoperative day. The dog has been clinically normal for 17 months.

DOI： 10.12935/jvma1951.54.697

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医師会  

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

Antihypertensive effect of exposure alternating current, high-voltage, electrical potential (AC-HVEP) were investigated in Dahl salt-sensitive (Dahl-S) rats used as models of salt-induced hypertension. The animals were divided into two groups: rats exposure to AC-HVEP (AC-HVEP group) and a control group not exposed to AC-HVEP. Throughout the experimental period, water with an addition of 1.0% NaC1 was given the animals. Progressive hypertension in the AC-HVEP group was significantly less serious than in the control group. In addition, histopathological examination showed greater restriction of lesions in the AC-HVEP than in the control group. Our results suggest that AC-HVEP exposure can suppress progressive hypertension secondary to chronic renal failure.

DOI： 10.12935/jvma1951.54.472

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医学会  

実験を3群に分け,コントロール群にはウサギ用標準飼料を8週間,コレステロール投与群には標準飼料に0.5%コレステロール及び0.25%胆汁酸を添加した高コレステロール飼料を8週間(実験群1)と15週間(実験群2),1日90g/例ずつ投与した.肺動脈圧は,収縮期,拡張期共に,実験群2が最も高く,次に実験群1,コントロール群の順であった.3群間に大きな差は認められなかったが,平均肺動脈圧のコントロール群と実験群2の間に統計学的有意差が認められた.組織学的には実験群1の肺動脈は幹気管支〜細気管支レベルの肺動脈を中心に,血管内皮下に軽度の限局性の泡沫細胞集簇が認められた.実験群2では細気管支レベルの肺動脈を中心に泡沫細胞の集簇により,中等度〜高度の血管内腔狭窄像が観察され,増殖細胞の一部は中膜から外膜に達していた.しかしながら,肺全体では高度病変は部分的には認められるものの,軽度病変が主体であった.今回の結果からコレステロール投与ウサギの肺動脈にも泡沫細胞増殖を主体とする動脈硬化病変が観察され,内膜肥厚及び血管内腔の高度狭窄も認められた.一方,肺高血圧を呈する程ではなかったものの,肺動脈圧は経時的に漸増した

Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPAN SOC VET SCI  

Previous studies have proposed that sodium supplement to nonionic contrast media (CM) can decrease the risk of ventricular fibrillation (VF). This study was designed to compare the occurence of VF induced by ioxilan (containing 9 mmol/LNa+) with other nonionic CMs. After wedging a catheter in the right coronary artery, test solutions including ioxilan, ioversol, iomeprol, and iopromide were infused for 30 sec at the rate of 0.4 ml/sec or until VF occurred. Then, incidence of VF, contact time (i.e. the time required to produce VF), and QTc were measured. Also, the CMs other than ioxilan were investigated at sodium levels adjusted to 9 and 20 mmol/L Na+. The incidence of VF with ioxilan (0%) was the lowest of all. In the other CMs, the incidence decreased in accordance with increase of sodium. Iomeprol and iopromide showed significant reduction of VF incidence at the sodium level of 20 mmol/L. The higher sodium supplements also prolonged the contact times. The increase of QTc was the greatest in ioxilan. Ioxilan has the least arrythmogenic property among the current low-osmolality nonionic CMs. This property might be attributable to an optimal sodium concentration of 9 mmol/L in the CM.

DOI： 10.1292/jvms.62.421

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本獣医師会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

A male mongrel cat (age 8 years and 5 months) was referred to our clinic because of syncopal episodes. Electrocardiography revealed paroxysmal third-degree atrioventricular block. Because syncopal episodes became increasingly frequent over a period of 5 months, permanent pacemaker implantion was performed. One year after the operation, no further syncopes had occurred. To the best of our knowledge, this is the first report in Japan of long-term observation of pacemaker implantation in a cat.

DOI： 10.12935/jvma1951.53.75

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To examine acute effects of olprinone hydrochloride (CAS 1067302-54-0, Coretec®) on pulmonary hypertension, hypoxic pulmonary hypertension was produced in 6 adult Beagle dogs. Using this pulmonary hypertension model, single intravenous bolus injections of olprinone at doses of 10, 30 and 100 μg/kg were administered at 5-min intervals and hemodynamic parameters were evaluated. Heart rate increased at doses of 30 and 100 μg/kg, but did not change at a dose of 10 μg/kg. Mean aortic pressure, mean pulmonary arterial pressure, pulmonary vascular resistance and systemic vascular resistance and right ventricular stroke work index did not change at doses of 10 and 30 μg/kg, but they decreased significantly at a dose of 100 μg/kg. On the other hand, cardiac index and the first derivative value of the left ventricular pressure did not show significant change at all doses. These results indicate the vasodilating effects on peripheral and pulmonary vessels in hypoxic model at high doses of olprinone. Its application in right heart failure accompanied by pulmonary hypertension therefore is expected to yield promising results.

DOI： 10.1055/s-0031-1300240

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：BRITISH VETERINARY ASSOC  

Uterine torsion secondary to sacculation of the uterine horns was diagnosed in two non-gravid bitches which were presented with anorexia, polydipsia and an acutely swollen abdomen, On the basis of the radiological and ultrasonographic findings, which indicated the presence of an enlarged spherical or tubular structure filled with hypoechoic material in the caudal abdomen, a tentative diagnosis of pyometra was made. Exploratory laparotomy revealed unilateral uterine horn torsion along the longitudinal axis, with bilateral fluid-filled sacculations. Ovariohysterectomy was performed in both cases. Pathological examination of the uteri demonstrated haematometra in one dog and pyometra in the other.

DOI： 10.1111/j.1748-5827.2000.tb03144.x

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本実験動物学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：INT PRESS EDITING CENTRE INC  

To examine both of the target vessels and the optimal time of their endothelial denudation to study vascular restenosis after balloon injury in cholesterol-loaded rabbits, we made 36 atherosclerotic rabbits by feeding a hypercholesterol diet, and histologically examined the onset time and the development of atherosclerosis. Atheromatous changes were observed first after the 5th week in the thoracic aorta from the start of the diet, and then extended to the abdominal aorta, coronary artery with time. The atherosclerotic lesions in the thoracic aorta and the proximal portion of the coronary artery showed high-grade concentric intimal thickening with luminal stenosis. The abdominal aortic lesion mildly progressed. In the renal, carotid and femoral arteries, in contrast, slight atheroscleromatous changes developed during the diet period. These results suggest that the thoracic and abdominal aortas and the coronary artery would be suitable as target vessels to study vascular restenosis after balloon injury, and the endothelial denudation of these vessels should be performed between the 8th and 15th week in this diet protocol for an accurate analysis.

DOI： 10.1538/expanim.48.1

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：(公社)日本実験動物学会  

36例のNZWウサギに0.5%コレステロール含有飼料を4〜15週間投与し,病変形成時期と進展経過について組織学的に検索した.病変形成時期は胸大動脈が投与5週目,腹大動脈が6週目,冠動脈が7週目,腎・総頸・大腿動脈が8週目と部位によって大きな差が認められた.胸大動脈・冠動脈の病変は経時的に進展し,中等度〜高度の内腔狭窄も認められたが,腹大動脈病変の進展経過は比較的緩やかであった.腎・総頸・大腿動脈の病変進展は極めて緩徐で,15週目でも局所病変にとどまった.この実験結果から,動脈硬化病変を有する血管を用いたballoon傷害後のrestenosisに関する研究では,cholesterol投与15週迄のモデルでも,胸大動脈・冠動脈・腹大動脈が対象血管となり得,それらの内皮剥離の至適時期は投与後8〜15週の間であることが推察された

Language：English   Publishing type：Research paper (scientific journal)  

His-bundle pacing gives a more physiological ventricular contraction in comparison to right ventricular apical pacing. However the problems of lead fixation and stability of long-term His-bundle pacing are yet unsolved. We used six adult beagles, in which a screw-in lead was anchored in the His- bundle region for observation of the pacing conditions and histopathologic changes of the conduction system over the course of 2 months. In the results, a satisfactory fixation was obtained using a conventional screw-in lead and no histological influence on the conduction system was observed. The pacing threshold at the time of implantation was 1.15 ± 0.69V (3.23 ± 3.08 mA) in the pulse width of 0.5 ms. R wave amplitude, the impedance and slew rate were 7.28 ± 2.04 mV, 409 ± 102 Ohm, and 0.65 ± 0.41 V/s, respectively. Two months later, these parameters changed to 2.83 ± 1.06 V (10.4 ± 5.71 mA), 5.63 ± 1.62 mV, 310 ± 71.3 Ohm, and 0.49 ± 0.22 V/s, respectively. These results suggest the feasibility of clinical application of permanent His- bundle pacing.

DOI： 10.1111/j.1540-8159.1999.tb00497.x

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Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Veterinary Medical Association  

A 15-day-old female calf complaining urinary obstruction and hematuria, was diagnosed as bladder rupture by positive-contrast cystography and ultrasonography. Through a posterior ventral midline incision one third of bladder containing ruptured devitalized tissue was debrided, resulting in recovery after operation. The case was suspected to be due to urachal anomaly type 2, a vesicourachal diverticulum.

DOI： 10.12935/jvma1951.51.246

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：International Press Editing Centre Incorporation  

To investigate the effects of medetomidine on late pregnant goats, medetomidine induced changes in maternal or fetal circulation and acid-base balance, as well as changes in intrauterine pressure (IUP) and uterine blood flow (UBF), were studied. Intramuscular administration of medetomidine (40 μg/kg b.w.) decreased the heart rate (HR) and arterial blood pressure (ABP) of the mother, and the change in HR was significant statistically (p&lt
0.05). In the fetus, HR and ABP showed a transient decrease and increase (p&lt
0.05), respectively. A decrease in maternal arterial blood pH and oxygen partial pressure (PO2) and an increase in carbon dioxide partial pressure (PCO2) were recorded alter the injection, but none was significant. In the fetus, arterial blood PO2 decreased significantly (p&lt
0.05) after 5 min of administration, and a significant metabolic acidemia supported by a decrease in base excess was observed. Within 1 to 4 min after the administration of medetomidine, IUP began to rise and remained high for 10 to 14 min. Thereafter, the rise in IUP was frequent and periodical. After the injection, UBF significantly (p&lt
0.05) decreased, and the fall in UBF was associated with a rise in IUP. The maternal and fetal serum medetomidine concentration increased remarkably after the injection of medetomidine into the mother. These observations in late pregnant goats suggested that medetomidine induced a decrease in maternal cardiac output, a decrease in UBF arising from the induction of uterine contractions, and transplacental medetomidine can have a suppressive effect on the fetus.

DOI： 10.1538/expanim.46.67

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Language：Japanese   Publisher：動物アレルギー検査(株)  

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

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DOI： 10.12935/jvma.69.143

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Language：English   Publisher：MDPI AG  

DOI： 10.3390/ijms15045410

Web of Science

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Language：Japanese   Publisher：(公財)鈴木謙三記念医科学応用研究財団  

Publisher：(株)文光堂  

Language：Japanese   Publisher：(株)文光堂  

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Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Publisher：(株)エデュワードプレス  

Language：Japanese   Publisher：(公社)日本獣医学会  

J-GLOBAL

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Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Publisher：(株)エデュワードプレス  

Language：Japanese   Publisher：(株)緑書房  

Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Publisher：(株)エデュワードプレス  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Publisher：(株)エデュワードプレス  

Language：Japanese   Publisher：(公社)日本獣医学会  

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Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese  

J-GLOBAL

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Language：Japanese  

J-GLOBAL

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Publisher：(一社)日本脳神経外傷学会  

Language：Japanese   Publisher：Japan Veterinary Medical Association  

An equine case of hind lameness and wobbler syndrome following an accidental forward fall during exercise was evaluated by diagnostic imaging. The myelography under general anesthesia was suggestive of a dorsally compressed spine between the sixth cervical vertebrae (C6) and C7. Histopathological findings also pointed to compression myelopathy between C6 and C7. A computed tomography scan of the cervical and thoracic (T) vertebraes following euthanasia was diagnostic of fractures of the C7 and joint space widening at the articular processes at the range from C6 to T2. Deformed alignment from C6 to T2 could induce spinal compression.

DOI： 10.12935/jvma.64.703

CiNii Books

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Publisher：(株)エデュワードプレス  

Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Japanese   Publisher：(株)メディカルレビュー社  

Language：English   Publisher：(公社)日本薬理学会  

J-GLOBAL

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J-GLOBAL

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Language：Japanese  

J-GLOBAL

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Language：Japanese   Publisher：(一社)日本脳神経外傷学会  

Publisher：(株)エデュワードプレス  

Language：Japanese   Publisher：(株)エデュワードプレス  

Language：Japanese   Publisher：(株)エデュワードプレス  

Language：English   Publisher：JAPAN SOC VET SCI  

Permanent pacemakers are commonly used in veterinary practice and can have a dramatic effect on the treatment of heart block. A Labrador Retriever dog suffering from exercise intolerance secondary to third degree atrioventricular block was treated with a new pacemaker system. A steroid-eluting screw-in type lead that has the advantage of being more fixed to the myocardial wall without increasing the pacing threshold was used. The heart rate was regulated with an acceleration sensing pacemaker generator that included several automatic modulation systems. Nineteen months after implantation, the dog has a normal level of activity. The present case suggests that this pacemaker design may offer important advantages for canine patients.

DOI： 10.1292/jvms.65.1101

Web of Science

Scopus

PubMed

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Language：English   Publishing type：Research paper, summary (international conference)   Publisher：BLACKWELL PUBLISHING INC  

Web of Science

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Language：Japanese  

DOI： 10.12935/jvma1951.54.837

CiNii Books

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Language：Japanese  

DOI： 10.12935/jvma1951.54.829

CiNii Books

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Language：Japanese   Publisher：Japan Veterinary Medical Association  

A three-year-old, female Shih Tzu with a history of dysphagia persisting since weaning had been kept on a liquid diet for about two years. The dog was referred to us because of difficulty in swallowing liquid food. Clinical signs, radiographic findings, and fluoroscopic examination after ingestion of food mixed with barium confirmed a diagnosis of cricopharyngeal achalasia. Four days later, we performed cricopharyngeal myotomy. After surgery, the dog no longer had difficulty swallowing food and was discharged on the third postoperative day. The dog has been clinically normal for 17 months.

DOI： 10.12935/jvma1951.54.697

CiNii Books

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Language：Japanese  

DOI： 10.12935/jvma1951.54.472

CiNii Books

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Language：Japanese  

DOI： 10.12935/jvma1951.53.75

CiNii Books

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Language：English   Publisher：JAPAN SOC VET SCI  

Previous studies have proposed that sodium supplement to nonionic contrast media (CM) can decrease the risk of ventricular fibrillation (VF). This study was designed to compare the occurence of VF induced by ioxilan (containing 9 mmol/LNa+) with other nonionic CMs. After wedging a catheter in the right coronary artery, test solutions including ioxilan, ioversol, iomeprol, and iopromide were infused for 30 sec at the rate of 0.4 ml/sec or until VF occurred. Then, incidence of VF, contact time (i.e. the time required to produce VF), and QTc were measured. Also, the CMs other than ioxilan were investigated at sodium levels adjusted to 9 and 20 mmol/L Na+. The incidence of VF with ioxilan (0%) was the lowest of all. In the other CMs, the incidence decreased in accordance with increase of sodium. Iomeprol and iopromide showed significant reduction of VF incidence at the sodium level of 20 mmol/L. The higher sodium supplements also prolonged the contact times. The increase of QTc was the greatest in ioxilan. Ioxilan has the least arrythmogenic property among the current low-osmolality nonionic CMs. This property might be attributable to an optimal sodium concentration of 9 mmol/L in the CM.

DOI： 10.1292/jvms.62.421

Web of Science

Scopus

PubMed

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Language：Japanese  

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Language：Japanese   Publisher：Japan Veterinary Medical Association  

A 15-day-old female calf complaining urinary obstruction and hematuria, was diagnosed as bladder rupture by positive-contrast cystography and ultrasonography. Through a posterior ventral midline incision one third of bladder containing ruptured devitalized tissue was debrided, resulting in recovery after operation. The case was suspected to be due to urachal anomaly type 2, a vesicourachal diverticulum.

DOI： 10.12935/jvma1951.51.246

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Event date： 2024.9

Language：English   Presentation type：Poster presentation  

Venue：Sapporo  

国際学会

Event date： 2024.7

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Venue：ホテルニューオオタニ（東京都）  

Event date： 2024.3

Language：English   Presentation type：Poster presentation  

Venue：Hotel New Otani, Tokyo   Country：Japan  

Event date： 2024.3

Language：English   Presentation type：Poster presentation  

Venue：Hotel New Otani, Tokyo   Country：Japan  

Event date： 2024.3

Language：English   Presentation type：Poster presentation  

Venue：Hotel New Otani, Tokyo   Country：Japan  

Event date： 2024.3

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Venue：鹿児島市  

Event date： 2024.2

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Venue：MEETING SPACE AP 品川（東京都）  

Event date： 2023.12

Language：English   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Venue：Municipal Council Hall, Çiğli Municipality Main Building, Izmir, Turkey   Country：Turkey  

Event date： 2023.12

Language：English   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Venue：Dokuz Eylul University, Izmir, Turkey   Country：Turkey  

Event date： 2023.11

Language：English   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Venue：Central Mindanao University, Republic of Philippines   Country：Philippines  

Event date： 2023.9

Language：English   Presentation type：Poster presentation  

Venue：Sapporo  

国際学会

Event date： 2023.9

Language：English   Presentation type：Poster presentation  

Venue：Sapporo  

国際学会

Event date： 2023.9

Language：English   Presentation type：Oral presentation (general)  

Venue：Sapporo  

国際学会

Event date： 2023.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：鹿児島県民交流センター（鹿児島市）  

Event date： 2023.6

Language：English   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Venue：University of the Philippines Los Banos, Republic of Philippines   Country：Philippines  

Event date： 2023.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：佐賀県教育会館（佐賀市）  

Event date： 2023.5

Language：English   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Event date： 2023.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：富士ソフトアキバプラザホール（東京都）  

Event date： 2022.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡国際会議場（福岡市）  

Event date： 2022.9 - 2022.10

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Japan  

Event date： 2022.9

Language：English   Presentation type：Symposium, workshop panel (nominated)  

Venue：Web (Balikesir, Turkey)   Country：Turkey  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Web(大分県獣医師会）  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Web(大分県獣医師会）  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Web(大分県獣医師会）  

Event date： 2022.5

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：バングラディシュ農業大学 (Web)  

国際学会

Event date： 2022.4

Language：English   Presentation type：Oral presentation (invited, special)  

Country：Turkey  

Event date： 2022.2

Language：English   Presentation type：Oral presentation (invited, special)  

Country：Turkey  

Event date： 2022.2

Language：English   Presentation type：Oral presentation (general)  

Event date： 2021.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Web  

Event date： 2021.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Web  

Event date： 2021.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Web  

Event date： 2021.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Web  

Event date： 2021.9

Language：English   Presentation type：Poster presentation  

Venue：web開催  

国際学会

Event date： 2021.9

Language：English   Presentation type：Poster presentation  

Venue：web開催  

国際学会

Event date： 2021.8 - 2021.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：web開催  

Event date： 2021.8 - 2021.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：web開催  

Event date： 2021.5

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：Web  

Event date： 2021.2

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：web開催  

国際学会

Event date： 2021.2

Language：English   Presentation type：Oral presentation (general)  

Venue：web開催  

国際学会

Event date： 2021.2

Language：English   Presentation type：Oral presentation (general)  

Venue：web開催  

国際学会

Event date： 2021.2

Language：English   Presentation type：Oral presentation (general)  

Venue：web開催  

国際学会

Event date： 2020.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山口大学（web開催）  

Event date： 2020.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山口大学（web開催）  

Event date： 2020.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山口大学（web開催）  

Event date： 2020.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山口大学（web開催）  

Event date： 2020.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山口大学（web開催）  

Event date： 2020.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山口大学（web開催）  

Event date： 2020.2 - 2020.3

Language：English   Presentation type：Oral presentation (keynote)  

Venue：バングラディシュ農業大学  

国際学会

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：グランデはがくれ（佐賀県）  

Event date： 2019.2

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：パシフィコ横浜  

Event date： 2018.11

Language：English   Presentation type：Symposium, workshop panel (nominated)  

Venue：Holiday Inn Singapore Atrium  

Event date： 2018.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡国際会議場