Updated on 2024/10/16

写真a

 
SAKAI Masao
 
Organization
Research Field in Agriculture, Agriculture, Fisheries and Veterinary Medicine Area Faculty of Agriculture Department of Agriculture Professor
Title
Professor
External link

Degree

  • 博士(農学) ( 1996.2   九州大学 )

Research Areas

  • Others / Others  / 環境微生物学

  • Others / Others  / 微生物生態学

  • Others / Others  / 土壌科学

  • Others / Others  / 土壌微生物学

Research History

  • Kagoshima University   Professor

    2005.12

  • Kagoshima University

    2005.12

Professional Memberships

  • 日本土壌微生物学会

    2015.10

  • 日本土壌肥料学会

    2015.10

  • 日本微生物生態学会

    2015.10

  • 日本農芸化学会

    2015.10

 

Papers

  • 竹腰 恵・池永 誠・富濵 毅・野口勝憲・境 雅夫 .  焼酎蒸留残液および化学合成殺菌剤による浸漬処理がジャガイモの種イモ共存細菌叢に与える影響 .  土と微生物76 ( 2 ) 68 - 76   2022.10焼酎蒸留残液および化学合成殺菌剤による浸漬処理がジャガイモの種イモ共存細菌叢に与える影響Reviewed

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    Authorship:Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Ikenaga Makoto, Kataoka Machi, Yin Xuan, Murouchi Aya, Sakai Masao .  Characterization and Distribution of Agar-degrading <i>Steroidobacter agaridevorans</i> sp. nov., Isolated from Rhizosphere Soils .  Microbes and Environments36 ( 1 ) n/a   2021

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    Authorship:Corresponding author   Language:English   Publisher:Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles  

    <p>The environment of plant rhizosphere soil differs from that of non-rhizosphere soil due to the secretion of mucilage polysaccharides from the roots. This environment is regarded as one of the preferential habitats for agar-degrading bacteria. In a previous study, agar-degrading <i>Steroidobacter agariperforans</i> KA5-B<sup>T</sup> was isolated from agar-enriched agricultural soil using diffusible metabolites from <i>Rhizobiales</i> bacteria. Based on the hypothesis that similar characteristic bacteria still exist in the rhizosphere, isolation was performed using rhizosphere soils. Agar-degrading SA29-B<sup>T</sup> and YU21-B were isolated from onion and soybean rhizosphere soils. The 16S rRNA genes of these strains showed ≥98.7% identities with the most closely related strain KA5-B<sup>T</sup>. However, differences were noted in polysaccharide utilization, and average nucleotide identities were <95–96% against strain KA5-B<sup>T</sup>, indicating that they are different species from <i>S. agariperforans</i> KA5-B<sup>T</sup>. To investigate the distribution of bacterial sequences affiliated with novel strains, a primer set was designed and a meta-analysis of the 16S rRNA gene was performed. Sequences were widely distributed in rhizospheres throughout Japan, but varied in plant- and region-dependent manners. Regarding phenotypic characterization, distinguishable features were observed in growth temperatures, pH, and dominant fatty acids. SA29-B<sup>T</sup> and YU21-B grew at 15–40°C and pH 6.0–12 and contained C<sub>16:0</sub> as the dominant cell fatty acid, whereas KA5-B<sup>T</sup> showed no growth at 40°C and pH 12 and contained a moderate amount of C<sub>16:0</sub>. Based on these characteristics, SA29-B<sup>T</sup> (JCM 333368<sup>T</sup>=KCTC 72223<sup>T</sup>) and YU21-B (JCM 333367=KCTC 72222) represent novel species in the genus <i>Steroidobacter</i>, for which the name <i>Steroidobacter agaridevorans</i> sp. nov. is proposed.</p>

    DOI: 10.1264/jsme2.ME20136

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  • 竹腰 恵,池永 誠,富濵 毅,野口勝憲,境 雅夫 .  焼酎蒸留残液によるジャガイモ種イモ浸漬処理が共存細菌群集およびそうか病菌に対する拮抗細菌に及ぼす影響 .  土と微生物74 ( 1 ) 32 - 41   2020.4焼酎蒸留残液によるジャガイモ種イモ浸漬処理が共存細菌群集およびそうか病菌に対する拮抗細菌に及ぼす影響Reviewed

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    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Furusawa A. .  Ralstonia solanacearum colonization of tomato roots infected by Meloidogyne incognita .  Journal of Phytopathology167 ( 6 ) 338 - 343   2019.6

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    Publisher:Journal of Phytopathology  

    DOI: 10.1111/jph.12804

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  • Makoto Ikenaga, Shohei Katsuragi, Yoshihiro Handa, Hiroshi Katsumata, Naoya Chishaki, Tomohiro Kawauchi, and Masao Sakai .  Improvements in Bacterial Primers to Enhance Selective SSU rRNA Gene Amplification of Plant-associated Bacteria by Applying the LNA Oligonucleotide-PCR Clamping Technique .  Microbes Environ.   2018Improvements in Bacterial Primers to Enhance Selective SSU rRNA Gene Amplification of Plant-associated Bacteria by Applying the LNA Oligonucleotide-PCR Clamping TechniqueReviewed

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  • Makoto Ikenaga, Masakazu Tabuchi, Tomohiro Kawauchi, Masao Sakai .  Application of Locked Nucleic Acid (LNA) Primer and PCR Clamping by LNA Oligonucleotide to Enhance the Amplification of Internal Transcribed Spacer (ITS) Regions in Investigating the Community Structures of Plant–Associated Fungi .  Microbes and Environments31 ( 3 ) 339 - 348   2016.9Application of Locked Nucleic Acid (LNA) Primer and PCR Clamping by LNA Oligonucleotide to Enhance the Amplification of Internal Transcribed Spacer (ITS) Regions in Investigating the Community Structures of Plant–Associated FungiReviewed

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  • Takashi Kunito, Yasutaka Ihyo, Hiromu Miyahara, Ryo Seta, Seishi Yoshida, Hiroyoshi Kubo, Kazunari Nagaoka, Masao Sakai, Kazutoshi Saeki .  Soil properties affecting adsorption of plasmid DNA and its transformation efficiency in Escherichia coli .  Biol Fertil Soils   2016Soil properties affecting adsorption of plasmid DNA and its transformation efficiency in Escherichia coliReviewed

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  • Tsuyoshi Tomihama, Yatsuka Nishi, Masao Sakai, Makoto Ikenaga, Takashi Okubo, Seishi Ikeda .  Draft Genome Sequences of Streptomyces scabiei S58, Streptomyces turgidiscabies T45, and Streptomyces acidiscabies a10, the Pathogens of Potato Common Scab, Isolated in Japan .  Genome Announcements4 ( 2 ) 1 - 2   2016Draft Genome Sequences of Streptomyces scabiei S58, Streptomyces turgidiscabies T45, and Streptomyces acidiscabies a10, the Pathogens of Potato Common Scab, Isolated in Japan

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  • Masao Sakai,Daigo Deguchi,Akifumi Hosoda,Tomohiro Kawauchi and Makoto Ikenaga .  Ammoniibacillus agariperforans gen. nov., sp. nov., a thermophilic, agar-degrading bacterium isolated from compost .  International Journal of Systematic and Evolutionary Microbiology65   570 - 577   2015.2Ammoniibacillus agariperforans gen. nov., sp. nov., a thermophilic, agar-degrading bacterium isolated from compostReviewed

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  • Makoto Ikenaga, Masakazu Tabuchi, Takuya Oyama, Isao Akagi and Masao Sakai .  Development of LNA oligonucleotide–PCR clamping technique in investigating the community structures of plant-associated bacteria .  Bioscience, Biotechnology, and Biochemistry79   1556 - 1566   2015Development of LNA oligonucleotide–PCR clamping technique in investigating the community structures of plant-associated bacteriaReviewed

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  • Makoto Ikenaga, Masao Sakai .  Application of Locked Nucleic Acid (LNA) Oligonucleotide - PCR Clamping Technique to Selectively PCR Amplify the SSU rRNA Genes of Bacteria in Investigating the Plant-Associated Community Structures .  Microbes and Environments29 ( 3 ) 286 - 295   2014.9Application of Locked Nucleic Acid (LNA) Oligonucleotide - PCR Clamping Technique to Selectively PCR Amplify the SSU rRNA Genes of Bacteria in Investigating the Plant-Associated Community StructuresReviewed

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  • Masao Sakai, Akifumi Hosoda, Kenjiro Ogura, Makoto Ikenaga .  The Growth of Steroidobacter agariperforans sp. nov., a Novel Agar-Degrading Bacterium Isolated from Soil, is Enhanced by the Diffusible Metabolites Produced by Bacteria Belonging to Rhizobiales .  Microbes and Environments29 ( 1 ) 89 - 95   2014.3The Growth of Steroidobacter agariperforans sp. nov., a Novel Agar-Degrading Bacterium Isolated from Soil, is Enhanced by the Diffusible Metabolites Produced by Bacteria Belonging to RhizobialesReviewed

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  • Masao Sakai, Makoto Ikenaga .  Application of peptide nucleic acid (PNA)-PCR clamping technique to investigate the community structures of rhizobacteria associated with plant roots .  Journal of Microbiological Methods92 ( 3 ) 281 - 288   2013.3Application of peptide nucleic acid (PNA)-PCR clamping technique to investigate the community structures of rhizobacteria associated with plant rootsReviewed

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  • Kazutoshi Saeki, Masao Sakai, Takashi Kunito .  Effect of α-casein on DNA adsorption by Andosols and by soil components .  Biology and Fertility of Soils48 ( 4 ) 469 - 474   2012.5Effect of α-casein on DNA adsorption by Andosols and by soil componentsReviewed

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  • Kazutoshi Saeki, Yasutaka Ihyo, Masao Sakai and Takashi Kunito .  Strong adsorption of DNA molecules on humic acids .  Environ. Chem. Lett.9 ( 4 ) 505 - 509   2011.12Strong adsorption of DNA molecules on humic acidsReviewed

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  • Kazutoshi Saeki, Takashi Kunito, Masao Sakai .  Effect of Tris-HCl buffer on DNA adsorption by a variety of soil constituents .  Microbes Environ. 26 ( 1 ) 88 - 91   2011.3Effect of Tris-HCl buffer on DNA adsorption by a variety of soil constituentsReviewed

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  • Kazutoshi Saeki, Masao Sakai, Shin-Ichiro Wada .  DNA adsorption on synthetic and natural allophanes .  Applied Clay Science50 ( 4 ) 493 - 497   2010.12DNA adsorption on synthetic and natural allophanesReviewed

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  • P.S. Sarr, T. Yamakawa, S. Asatsuma, S. Fujimoto and M. Sakai .  Investigation of endophytic and symbiotic features of Ralstonia sp. TSC1 isolated from cowpea nodules .  African Journal of Microbiology Research4 ( 19 ) 1959 - 1963   2010.10Investigation of endophytic and symbiotic features of Ralstonia sp. TSC1 isolated from cowpea nodulesReviewed

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  • Kazutoshi Saeki, Takashi Kunito, Masao Sakai .  Effects of pH, ionic strength, and solutes on DNA adsorption .  Biol. Fertil. Soils46 ( 5 ) 531 - 535   2010.6Effects of pH, ionic strength, and solutes on DNA adsorptionReviewed

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  • Masunaka, A., Nakaho, K., Sakai, M., Takahashi, H. and Takenaka, S. .  Visualization of Ralstonia solanacearum cells during biocontrol of bacterial wilt disease in tomato with Pythium oligandrum .  J. Gen. Plant Pathol.75 ( 4 ) 281 - 287   2009.8Visualization of Ralstonia solanacearum cells during biocontrol of bacterial wilt disease in tomato with Pythium oligandrumReviewed

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  • Saeki, K. and Sakai, M .  The influence of soil organic matter on DNA adsorptions on andosols .  Microbes and Environments24 ( 2 ) 175 - 179   2009.6The influence of soil organic matter on DNA adsorptions on andosolsReviewed

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  • Saeki, K., Morisaki, M. and Sakai, M. .  The contribution of soil constituents to adsorption of extracellular DNA by soils. .  Microbes and Environments23 ( 4 ) 353 - 355   2008.12The contribution of soil constituents to adsorption of extracellular DNA by soils.Reviewed

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  • Hirohito TSURUMARU, Takeo YAMAKAWA, Masayuki TANAKA and Masao SAKAI .  Tn5 mutants of Bradyrhizobium japonicum Is-1 with altered compatibility with Rj2-soybean cultivars .  Soil Science and Plant Nutrition54 ( 2 ) 197 - 203   2008.4Tn5 mutants of Bradyrhizobium japonicum Is-1 with altered compatibility with Rj2-soybean cultivarsReviewed

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  • Hirohito Tsurumaru, Takeo Yamakawa, Masayuki Tanaka, Masao Sakai .  The Efficient Strategy of Plasmid Rescue from Tn5 Mutants Derived from Bradyrhizobium japonicum Is-1, Based on Whole Genome Sequence Information of Strain USDA110 .  Journal of the Faculty of Agriculture, Kyushu University 53 ( 1 ) 27 - 31   2008.2The Efficient Strategy of Plasmid Rescue from Tn5 Mutants Derived from Bradyrhizobium japonicum Is-1, Based on Whole Genome Sequence Information of Strain USDA110Reviewed

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  • Tanaka,K., Shimizu,T., Zakria,M., Njoloma,J., Saeki,Y., Sakai,M., Yamakawa,T., Minamisawa,K., Akao,S. .  Incorporation of a DNA sequence encoding green fluorescent protein (GFP) into endophytic diazotroph from sugarcane and sweet potato and the colonizing ability of these bacteria in Brassica oleracea .  Microbes and Environments21 ( 2 ) 122 - 128   2006.6Incorporation of a DNA sequence encoding green fluorescent protein (GFP) into endophytic diazotroph from sugarcane and sweet potato and the colonizing ability of these bacteria in Brassica oleraceaReviewed

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  • Hosoda, A., Sakai, M .  Isolation of Asticcacaulis sp. SA7, a novel agar-degrading Alphaproteobacterium .  Bioscience Biotechnology and Biochemistry70 ( 3 ) 722 - 725   2006.3Isolation of Asticcacaulis sp. SA7, a novel agar-degrading AlphaproteobacteriumReviewed

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  • Urashima, Y., Sakai, M., Suga, Y., Fukunaga, A. and Hori, K. .  Gravitational water flow enhances the colonization of spinach roots in soil by plant growth-promoting Pseudomonas .  Soil Sci. Plant Nutr.50 ( 2 ) 277 - 281   2004.3Gravitational water flow enhances the colonization of spinach roots in soil by plant growth-promoting PseudomonasReviewed

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  • Sakai, M., Matsuka, A., Komura, T. and Kanazawa, S. .  Application of a new PCR primer for terminal restriction fragment length polymorphism analysis of the bacterial communities in plant roots .  J. Microbiol. Methods59 ( 1 ) 81 - 89   2004Application of a new PCR primer for terminal restriction fragment length polymorphism analysis of the bacterial communities in plant rootsReviewed

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  • Sakai, M., Saeki, M., Sakamoto, A. and Kanazawa, S. .  Biodegradability of photodegradable coating materials used for coated fertilizers in agricultural soils .  Soil Sci. Plant Nutr.49 ( 4 ) 647 - 650   2003.7Biodegradability of photodegradable coating materials used for coated fertilizers in agricultural soilsReviewed

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  • Matsuka, A., Sakai, M. and Kanazawa, S. .  Application of T-RFLP analysis for bacterial community structure of colonies grown on agar plates .  J. Fac. Agr. Kyushu Univ.48 ( 1,2 ) 107 - 112   2003Application of T-RFLP analysis for bacterial community structure of colonies grown on agar platesReviewed

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  • Sakai, M., Futamata, H. and Kanazawa, S. .  Effects of high concentrations of inorganic salts on swarming ability in Pseudomonas strains .  Biosci. Biotechnol. Biochem.67 ( 7 ) 1479 - 1484   2003Effects of high concentrations of inorganic salts on swarming ability in Pseudomonas strainsReviewed

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  • Hosoda, A., Sakai, M. and Kanazawa, S. .  Isolation and characterization of agar-degrading Paenibacillus spp. associated with the rhizosphere of spinach .  Biosci. Biotechnol. Biochem.67 ( 5 ) 1048 - 1055   2003Isolation and characterization of agar-degrading Paenibacillus spp. associated with the rhizosphere of spinachReviewed

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